WO2001068900A3 - Verfahren zum spezifischen nachweis von mikroorganismen durch polymerase-kettenreaktion - Google Patents

Verfahren zum spezifischen nachweis von mikroorganismen durch polymerase-kettenreaktion Download PDF

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Publication number
WO2001068900A3
WO2001068900A3 PCT/EP2001/002949 EP0102949W WO0168900A3 WO 2001068900 A3 WO2001068900 A3 WO 2001068900A3 EP 0102949 W EP0102949 W EP 0102949W WO 0168900 A3 WO0168900 A3 WO 0168900A3
Authority
WO
WIPO (PCT)
Prior art keywords
specifically detecting
chain reaction
polymerase chain
detecting microorganisms
microorganisms
Prior art date
Application number
PCT/EP2001/002949
Other languages
English (en)
French (fr)
Other versions
WO2001068900A2 (de
Inventor
Marion Walcher
Michael Wagner
Jiri Snaidr
Original Assignee
Vermicon Ag
Marion Walcher
Michael Wagner
Jiri Snaidr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vermicon Ag, Marion Walcher, Michael Wagner, Jiri Snaidr filed Critical Vermicon Ag
Priority to AU2001262096A priority Critical patent/AU2001262096A1/en
Publication of WO2001068900A2 publication Critical patent/WO2001068900A2/de
Publication of WO2001068900A3 publication Critical patent/WO2001068900A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zum spezifischen Nachweis von Mikroorganismen und zu deren Unterscheidung von nahe verwandten Mikroorganismen in einer Probe durch Polymerase-Kettenreaktion (PCR). Insbesondere betrifft die Erfindung ein Verfahren zum spezifischen Nachweis von Mikroorganismen der Gattung Listeria unterhalb der Artebene sowie Primer, die bei diesem Verfahren eingesetzt werden können.
PCT/EP2001/002949 2000-03-15 2001-03-15 Verfahren zum spezifischen nachweis von mikroorganismen durch polymerase-kettenreaktion WO2001068900A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001262096A AU2001262096A1 (en) 2000-03-15 2001-03-15 Method for specifically detecting microorganisms by polymerase chain reaction

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10012540.9 2000-03-15
DE10012540A DE10012540B4 (de) 2000-03-15 2000-03-15 Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion

Publications (2)

Publication Number Publication Date
WO2001068900A2 WO2001068900A2 (de) 2001-09-20
WO2001068900A3 true WO2001068900A3 (de) 2002-08-22

Family

ID=7634767

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/002949 WO2001068900A2 (de) 2000-03-15 2001-03-15 Verfahren zum spezifischen nachweis von mikroorganismen durch polymerase-kettenreaktion

Country Status (3)

Country Link
AU (1) AU2001262096A1 (de)
DE (1) DE10012540B4 (de)
WO (1) WO2001068900A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005516627A (ja) * 2002-02-04 2005-06-09 バーミコン　アクチェンゲゼルシャフト 食品に関連する病原性細菌を特異的に迅速に検出する方法
DE102007021387A1 (de) 2007-05-04 2008-11-06 Eads Deutschland Gmbh Detektionsvorrichtung zur Detektion von biologischen Mikropartikeln wie Bakterien, Viren, Sporen, Pollen oder biologische Toxine, sowie Detektionsverfahren
WO2011112534A1 (en) 2010-03-08 2011-09-15 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence
CN102906106B (zh) * 2010-06-02 2015-11-25 默克专利股份公司 单核细胞增生李斯特菌物种的遗传修饰细菌
AU2012223438A1 (en) * 2011-02-28 2013-09-26 Transgenomic, Inc Kit and method for sequencing a target DNA in a mixed population
EP3333269B1 (de) 2011-03-31 2021-05-05 Dana-Farber Cancer Institute, Inc. Verfahren zur ermöglichung einer multiplex-kalt-pcr
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
CN105821123A (zh) * 2016-04-01 2016-08-03 刘二龙 基于单增李斯特菌毒力基因的三重实时荧光定量pcr检测用引物、mgb探针及检测方法
EP3551756A4 (de) 2016-12-12 2020-07-15 Dana Farber Cancer Institute, Inc. Zusammensetzungen und verfahren zum molekularen barcoding von dna-molekülen vor der mutationsanreicherung und/oder der mutationsdetektion
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH682156A5 (en) * 1990-06-28 1993-07-30 Urs Candrian Listeria monocytogenes detection by enzymatic nucleic acid amplification - using oligo-nucleotide(s) derived from alpha-haemolysin and/or beta-haemo-lysin virulence factors in polymerase chain reactions
EP0576842A2 (de) * 1992-06-11 1994-01-05 MERCK PATENT GmbH Verfahren und Mittel zum Nachweis von Listerien
GB2293238A (en) * 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
EP0725148A2 (de) * 1994-11-30 1996-08-07 Becton, Dickinson and Company Amplifikation und Nachweis von mycobacteriellen Nukleinsäuren
EP0799888A1 (de) * 1994-12-09 1997-10-08 Wakunaga Seiyaku Kabushiki Kaisha Verfahren zur hemmung von nichtspezifischer hybridisierung in primerverlängerungsreaktionen
WO1998044153A1 (en) * 1997-03-27 1998-10-08 Institute Of Environmental Science & Research Limited DETECTION OF LISTERIA MONOCYTOGENES, LISTERIA SPP., AND $i(RHODOCOCCUS COPROPHILUS)
FR2779154A1 (fr) * 1998-05-27 1999-12-03 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5948618A (en) * 1995-01-27 1999-09-07 Wakunaga Seiyaku Kabushiki Kaisha Primer for gene amplification, method for nucleic acid discrimination with the use of the same, and nucleic acid discrimination kit
US5627054A (en) * 1996-04-05 1997-05-06 The United States Of America As Represented By The Secretary Of The Army Competitor primer asymmetric polymerase chain reaction
GB9807045D0 (en) * 1998-04-01 1998-06-03 Rudi Knut Nucleic acid detection method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH682156A5 (en) * 1990-06-28 1993-07-30 Urs Candrian Listeria monocytogenes detection by enzymatic nucleic acid amplification - using oligo-nucleotide(s) derived from alpha-haemolysin and/or beta-haemo-lysin virulence factors in polymerase chain reactions
EP0576842A2 (de) * 1992-06-11 1994-01-05 MERCK PATENT GmbH Verfahren und Mittel zum Nachweis von Listerien
GB2293238A (en) * 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
EP0725148A2 (de) * 1994-11-30 1996-08-07 Becton, Dickinson and Company Amplifikation und Nachweis von mycobacteriellen Nukleinsäuren
EP0799888A1 (de) * 1994-12-09 1997-10-08 Wakunaga Seiyaku Kabushiki Kaisha Verfahren zur hemmung von nichtspezifischer hybridisierung in primerverlängerungsreaktionen
WO1998044153A1 (en) * 1997-03-27 1998-10-08 Institute Of Environmental Science & Research Limited DETECTION OF LISTERIA MONOCYTOGENES, LISTERIA SPP., AND $i(RHODOCOCCUS COPROPHILUS)
FR2779154A1 (fr) * 1998-05-27 1999-12-03 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre

Also Published As

Publication number Publication date
DE10012540B4 (de) 2004-09-23
DE10012540A1 (de) 2001-09-27
WO2001068900A2 (de) 2001-09-20
AU2001262096A1 (en) 2001-09-24

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