WO2001062781A3 - Homogentisatphytyltransferase - Google Patents

Homogentisatphytyltransferase Download PDF

Info

Publication number
WO2001062781A3
WO2001062781A3 PCT/EP2001/001720 EP0101720W WO0162781A3 WO 2001062781 A3 WO2001062781 A3 WO 2001062781A3 EP 0101720 W EP0101720 W EP 0101720W WO 0162781 A3 WO0162781 A3 WO 0162781A3
Authority
WO
WIPO (PCT)
Prior art keywords
phytyl transferase
homogentisate phytyl
nucleic acid
homogentisate
acid sequences
Prior art date
Application number
PCT/EP2001/001720
Other languages
English (en)
French (fr)
Other versions
WO2001062781A2 (de
Inventor
Karin Herbers
Ralf Badur
Irene Kunze
Susanne Sommer
Rainer Lemke
Michael Geiger
Original Assignee
Sungene Gmbh & Co Kgaa
Karin Herbers
Ralf Badur
Irene Kunze
Susanne Sommer
Rainer Lemke
Michael Geiger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungene Gmbh & Co Kgaa, Karin Herbers, Ralf Badur, Irene Kunze, Susanne Sommer, Rainer Lemke, Michael Geiger filed Critical Sungene Gmbh & Co Kgaa
Priority to EP01923581A priority Critical patent/EP1299413A2/de
Priority to AU2001250321A priority patent/AU2001250321A1/en
Priority to CA002400783A priority patent/CA2400783A1/en
Priority to BR0108649-9A priority patent/BR0108649A/pt
Publication of WO2001062781A2 publication Critical patent/WO2001062781A2/de
Publication of WO2001062781A3 publication Critical patent/WO2001062781A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
    • C07D311/70—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with two hydrocarbon radicals attached in position 2 and elements other than carbon and hydrogen in position 6
    • C07D311/72—3,4-Dihydro derivatives having in position 2 at least one methyl radical and in position 6 one oxygen atom, e.g. tocopherols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Die Erfindung betrifft Nukleinsäuresequenzen kodierend ein Protein mit Homogentisatphytyltransferase-Aktivität, die Verwendung der Nukleinsäuren zur Herstellung von transgenen Organismen, wie beispielsweisetransgenen Pflanzen mit erhöhtem Gehalt an Tocopherolen und Tocotrienolen, ein Verfahren zur Herstellung von Pflanzen mit erhöhtem Gehalt an Tocopherolen und/oder Tocotrienolen, sowie die transgenen Pflanzen selbst.
PCT/EP2001/001720 2000-02-25 2001-02-16 Homogentisatphytyltransferase WO2001062781A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP01923581A EP1299413A2 (de) 2000-02-25 2001-02-16 Homogentisatphytyltransferase
AU2001250321A AU2001250321A1 (en) 2000-02-25 2001-02-16 Homogentisate phytyl transferase
CA002400783A CA2400783A1 (en) 2000-02-25 2001-02-16 Homogentisate phytyl transferase
BR0108649-9A BR0108649A (pt) 2000-02-25 2001-02-16 Construção de ácido nucléico, organismo geneticamente modificado, uso de um organismo geneticamente modificado, método para a geração de organismos geneticamente modificados, uso da construção do ácido nucléico, e, processo para a produção de vitamina e

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10009002A DE10009002A1 (de) 2000-02-25 2000-02-25 Homogentisatphytyltransferase
DE10009002.8 2000-02-25

Publications (2)

Publication Number Publication Date
WO2001062781A2 WO2001062781A2 (de) 2001-08-30
WO2001062781A3 true WO2001062781A3 (de) 2002-01-24

Family

ID=7632465

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/001720 WO2001062781A2 (de) 2000-02-25 2001-02-16 Homogentisatphytyltransferase

Country Status (7)

Country Link
US (1) US20030084479A1 (de)
EP (1) EP1299413A2 (de)
AU (1) AU2001250321A1 (de)
BR (1) BR0108649A (de)
CA (1) CA2400783A1 (de)
DE (1) DE10009002A1 (de)
WO (1) WO2001062781A2 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002541851A (ja) 1999-04-15 2002-12-10 カルジーン エルエルシー イソプレノイド合成に関与するタンパク質の核酸配列
US6787683B1 (en) 1999-05-07 2004-09-07 Pioneer Hi-Bred International, Inc. Phytyl/prenyltransferase nucleic acids, polypeptides and uses thereof
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
CA2418436C (en) 2000-08-07 2017-07-11 Monsanto Technology, Llc Methyl-d-erythritol phosphate pathway genes
DE10046462A1 (de) * 2000-09-19 2002-05-29 Sungene Gmbh & Co Kgaa Verbesserte Verfahren zur Vitamin E Biosynthese
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
ATE419366T1 (de) 2001-05-09 2009-01-15 Monsanto Technology Llc Tyra-gene und ihre verwendung
US7244877B2 (en) 2001-08-17 2007-07-17 Monsanto Technology Llc Methyltransferase from cotton and uses thereof
AR036962A1 (es) 2001-10-25 2004-10-13 Monsanto Technology Llc Metiltransferasas aromaticas y usos de las mismas
BRPI0308740B1 (pt) 2002-03-19 2018-11-21 Monsanto Technology Llc molécula de ácido nucléico codificando uma homogentisato prenil transferase (“hpt”) e método de produzir planta tendo semente com nível de tocoferol aumentado
DE10212703A1 (de) * 2002-03-21 2003-10-02 Sungene Gmbh & Co Kgaa Erhöhung Vitamin-E-Gehalts in Organismen durch Erhöhung der 2-Methyl-6-phytythydrochinon-Methyltransferase-Aktivität
DE10231587A1 (de) * 2002-07-11 2004-01-29 Sungene Gmbh & Co. Kgaa Transgene Expressionskonstrukte und Verfahren zum Erhöhen des Vitamin E-Gehaltes in pflanzlichen Organismen
DE10232483A1 (de) * 2002-07-17 2004-02-05 Sungene Gmbh & Co. Kgaa Transgene Expressionskonstrukte und Verfahren zum Erhöhen des Vitamin E-Gehaltes in pflanzlichen Organismen
EP1546334A4 (de) 2002-08-05 2007-01-03 Monsanto Technology Llc Gene in verbindung mit tocopherol-biosynthese und verwendungen davon
EP1871883A1 (de) * 2005-03-02 2008-01-02 Metanomics GmbH Verfahren zur produktion von feinchemikalien

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000063391A2 (en) * 1999-04-15 2000-10-26 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
WO2000068393A1 (en) * 1999-05-07 2000-11-16 Pioneer Hi-Bred International, Inc. Phytyl/prenyltransferase nucleic acids, polypeptides and uses thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618988A (en) * 1990-03-02 1997-04-08 Amoco Corporation Enhanced carotenoid accumulation in storage organs of genetically engineered plants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000063391A2 (en) * 1999-04-15 2000-10-26 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
WO2000068393A1 (en) * 1999-05-07 2000-11-16 Pioneer Hi-Bred International, Inc. Phytyl/prenyltransferase nucleic acids, polypeptides and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 1 February 1997 (1997-02-01), KANEKO T ET AL.: "Hypothetical 34.4 kDa Protein", XP002173642 *
KANEKO T ET AL.: "Sequence analysis of the genome of teh unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignement of potential protein-coding regions", DNE RESEARCH, vol. 3, no. 3, 1996, pages 109 - 136, XP002084893 *
KELLER YVES ET AL: "Metabolic compartmentation of plastid prenyllipid biosynthesis: Evidence for the involvement of a multifunctional geranylgeranyl reductase.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 251, no. 1-2, January 1998 (1998-01-01), pages 413 - 417, XP002173641, ISSN: 0014-2956 *
NORRIS SUSAN R ET AL: "Complementation of the arabidopsis pds1 mutation with the gene encoding p-hydroxyphenylpyruvate dioxygenase", PLANT PHYSIOLOGY,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD,US, vol. 117, no. 4, August 1998 (1998-08-01), pages 1317 - 1323, XP002146400, ISSN: 0032-0889 *
SCHLEDZ M ET AL: "A novel phytyltransferase from Synechocystis sp. PCC 6803 involved in tocopherol biosynthesis", FEBS LETTERS,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM,NL, vol. 499, no. 1-2, 15 June 2001 (2001-06-15), pages 15 - 20, XP004246978, ISSN: 0014-5793 *
SHINTANI D ET AL: "ELEVATING THE VITAMIN E CONTENT OF PLANTS THROUGH METABOLIC ENGINEERING", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 282, no. 282, 1998, pages 2098 - 2100, XP000887122, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
US20030084479A1 (en) 2003-05-01
CA2400783A1 (en) 2001-08-30
EP1299413A2 (de) 2003-04-09
AU2001250321A1 (en) 2001-09-03
DE10009002A1 (de) 2001-08-30
WO2001062781A2 (de) 2001-08-30
BR0108649A (pt) 2003-04-29

Similar Documents

Publication Publication Date Title
WO2001062781A3 (de) Homogentisatphytyltransferase
WO2003004659A8 (de) Rekombinationssysteme und verfahren zum entfernen von nukleinsäuresequenzen aus dem genom eukaryotischer organismen
ATE280832T1 (de) Methode zur herstellung einer dürre-resistenten pflanze
WO2001039599A3 (en) Biological control by conditional dominant lethal genetic system
WO2005002359A3 (en) Modified starch, uses, methods for production thereof
ATE551352T1 (de) Peptidakzeptorbindungsverfahren
WO2002076205A3 (en) Compositions and methods of zearalenone detoxification
WO2003014307A3 (en) Antisense modulation of apolipoprotein(a) expression
WO2003004614A3 (en) Identification and use of conserved noncoding sequences
WO2003054229A3 (fr) Polymorphismes du gene ccoaomt2 de mais, et leurs utilisations pour ameliorer la digestibilite des plantes.
ATE430803T1 (de) Gene, welche für flavon-synthasen kodieren
WO2004087755A3 (en) Plant haemoglobin
ATE263161T1 (de) Verfahren zur herstellung des alpha-tocopherol acetats
WO2004074442A3 (en) Plant regulatory sequences for selective control of gene expression
WO2001016303A3 (de) Nucleinsäuren, die für enzymaktivitäten der spinosyn-biosynthese codieren
AU2001270244A1 (en) Plant regulatory sequences for selective control of gene expression
WO2002101064A3 (en) Process for the preparation of d-pantothenic acid and/or salts thereof
WO2002068682A3 (de) Verfahren zur identifizierung biologisch aktiver strukturen mikrobieller erreger
WO2003000930A3 (en) Nucleotide sequences involved in plant disease resistance
WO2002048321A3 (de) Verwendung von acetyl-coa carboxylase zum identifizieren von insektizid wirksamen verbindungen
WO2000065073A3 (de) Genetische sequenz, die für flavonsynthase ii enzyme kodiert, und deren verwendung
AU2001257385A1 (en) Plant regulatory sequences for selective control of gene expression
WO2002006487A3 (de) Regulatorische sequenzen aus meristematischen zellen
WO2003008588A3 (de) Verfahren zur herstellung von 2-keto-l-gulonsäure und vitamin c unter benutzung von l-sorbose-und l-sorbosondehydrogenase
WO2002050293A3 (de) Pflanzen mit verbesserter widerstandskraft

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 2001923581

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2400783

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 10204700

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 2001923581

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001923581

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP