WO2001046408A2 - Molecular clones with mutated hiv gag/pol, siv gag and siv env genes - Google Patents

Molecular clones with mutated hiv gag/pol, siv gag and siv env genes Download PDF

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Publication number
WO2001046408A2
WO2001046408A2 PCT/US2000/034985 US0034985W WO0146408A2 WO 2001046408 A2 WO2001046408 A2 WO 2001046408A2 US 0034985 W US0034985 W US 0034985W WO 0146408 A2 WO0146408 A2 WO 0146408A2
Authority
WO
WIPO (PCT)
Prior art keywords
hiv
gag
nucleic acid
mammal
acid construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/034985
Other languages
English (en)
French (fr)
Other versions
WO2001046408A3 (en
WO2001046408A9 (en
Inventor
George N. Pavlakis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Health and Human Services
Original Assignee
US Department of Health and Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2001546906A priority Critical patent/JP4627950B2/ja
Application filed by US Department of Health and Human Services filed Critical US Department of Health and Human Services
Priority to AU25923/01A priority patent/AU785133B2/en
Priority to EP00989415A priority patent/EP1246913B1/en
Priority to AT00989415T priority patent/ATE438714T1/de
Priority to DE60042702T priority patent/DE60042702D1/de
Priority to CA2395269A priority patent/CA2395269C/en
Priority to US09/872,733 priority patent/US6656706B2/en
Publication of WO2001046408A2 publication Critical patent/WO2001046408A2/en
Publication of WO2001046408A3 publication Critical patent/WO2001046408A3/en
Anticipated expiration legal-status Critical
Priority to US10/263,020 priority patent/US8076100B2/en
Publication of WO2001046408A9 publication Critical patent/WO2001046408A9/en
Priority to US10/644,027 priority patent/US7608422B2/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/21—Retroviridae, e.g. equine infectious anemia virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Communicable Diseases (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
PCT/US2000/034985 1999-12-23 2000-12-22 Molecular clones with mutated hiv gag/pol, siv gag and siv env genes Ceased WO2001046408A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
CA2395269A CA2395269C (en) 1999-12-23 2000-12-22 Molecular clones with mutated hiv gag/pol, siv gag and siv env genes
AU25923/01A AU785133B2 (en) 1999-12-23 2000-12-22 Molecular clones with mutated HIV GAG/POL, SIV GAG and SIV ENV genes
EP00989415A EP1246913B1 (en) 1999-12-23 2000-12-22 Molecular clones with mutated hiv gag/pol, siv gag and siv env genes
AT00989415T ATE438714T1 (de) 1999-12-23 2000-12-22 Molekulare klone mit mutierten hiv gag/pol, siv gag und siv env gene
DE60042702T DE60042702D1 (de) 1999-12-23 2000-12-22 Molekulare klone mit mutierten hiv gag/pol, siv gag und siv env gene
JP2001546906A JP4627950B2 (ja) 1999-12-23 2000-12-22 変異ＨＩＶｇａｇ／ｐｏｌ、ＳＩＶｇａｇおよびＳＩＶｅｎｖ遺伝子を持つ分子クローン
US09/872,733 US6656706B2 (en) 1999-12-23 2001-06-01 Molecular clones with mutated HIV gag/pol, SIV gag and SIV env genes
US10/263,020 US8076100B2 (en) 1999-12-23 2002-10-02 Molecular clones with mutated HIV gag/pol, SIV gag and SIV env genes
US10/644,027 US7608422B2 (en) 1999-12-23 2003-08-19 Simian immunodeficiency virus (SIV) molecular clone encoding mutant gag gene lacking inhibitory/instability regions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17303699P 1999-12-23 1999-12-23
US60/173,036 1999-12-23

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/872,733 Continuation-In-Part US6656706B2 (en) 1999-12-23 2001-06-01 Molecular clones with mutated HIV gag/pol, SIV gag and SIV env genes

Publications (3)

Publication Number Publication Date
WO2001046408A2 true WO2001046408A2 (en) 2001-06-28
WO2001046408A3 WO2001046408A3 (en) 2001-12-13
WO2001046408A9 WO2001046408A9 (en) 2002-11-07

Family

ID=22630264

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/034985 Ceased WO2001046408A2 (en) 1999-12-23 2000-12-22 Molecular clones with mutated hiv gag/pol, siv gag and siv env genes

Country Status (8)

Country Link
EP (2) EP1246913B1 (enExample)
JP (1) JP4627950B2 (enExample)
AT (1) ATE438714T1 (enExample)
AU (1) AU785133B2 (enExample)
CA (1) CA2395269C (enExample)
DE (1) DE60042702D1 (enExample)
ES (2) ES2329648T3 (enExample)
WO (1) WO2001046408A2 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003095605A3 (en) * 2002-05-08 2004-02-19 Pharmacia Italia Spa A chimeric reverse transcriptase and methods for identifying telomerase inhibitors
EP2116605A2 (en) 2004-06-17 2009-11-11 Wyeth Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV
US7943375B2 (en) 1998-12-31 2011-05-17 Novartis Vaccines & Diagnostics, Inc Polynucleotides encoding antigenic HIV type C polypeptides, polypeptides and uses thereof
US8133494B2 (en) 2001-07-05 2012-03-13 Novartis Vaccine & Diagnostics Inc Expression cassettes endcoding HIV-1 south african subtype C modified ENV proteins with deletions in V1 and V2

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1980617A1 (en) 1998-12-31 2008-10-15 Novartis Vaccines and Diagnostics, Inc. Improved expression of HIV polypeptides and production of virus-like particles
US6656706B2 (en) * 1999-12-23 2003-12-02 The United States Of America As Represented By The Department Of Health And Human Services Molecular clones with mutated HIV gag/pol, SIV gag and SIV env genes
US20030170614A1 (en) 2001-08-31 2003-09-11 Megede Jan Zur Polynucleotides encoding antigenic HIV type B polypeptides, polypeptides and uses thereof
GB201501523D0 (en) * 2015-01-30 2015-03-18 Univ Plymouth Differential immune response modulation
US11820999B2 (en) * 2017-04-03 2023-11-21 American Gene Technologies International Inc. Compositions and methods for treating phenylketonuria

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6174666B1 (en) * 1992-03-27 2001-01-16 The United States Of America As Represented By The Department Of Health And Human Services Method of eliminating inhibitory/instability regions from mRNA
EP1080201B1 (en) * 1998-05-22 2008-01-23 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Novel post-transcriptional regulatory elements and uses thereof
EP1980617A1 (en) * 1998-12-31 2008-10-15 Novartis Vaccines and Diagnostics, Inc. Improved expression of HIV polypeptides and production of virus-like particles
IL146090A0 (en) * 1999-04-26 2002-07-25 Leuven K U Res & Dev A synthetic gene for expression of a retroviral protein with wild type activity in eukaryotic cells

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7943375B2 (en) 1998-12-31 2011-05-17 Novartis Vaccines & Diagnostics, Inc Polynucleotides encoding antigenic HIV type C polypeptides, polypeptides and uses thereof
US8133494B2 (en) 2001-07-05 2012-03-13 Novartis Vaccine & Diagnostics Inc Expression cassettes endcoding HIV-1 south african subtype C modified ENV proteins with deletions in V1 and V2
WO2003095605A3 (en) * 2002-05-08 2004-02-19 Pharmacia Italia Spa A chimeric reverse transcriptase and methods for identifying telomerase inhibitors
EP2116605A2 (en) 2004-06-17 2009-11-11 Wyeth Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV

Also Published As

Publication number Publication date
JP2003517839A (ja) 2003-06-03
EP2128256A3 (en) 2009-12-23
ATE438714T1 (de) 2009-08-15
ES2329648T3 (es) 2009-11-30
EP1246913B1 (en) 2009-08-05
EP1246913A2 (en) 2002-10-09
CA2395269A1 (en) 2001-06-28
CA2395269C (en) 2015-12-08
DE60042702D1 (de) 2009-09-17
WO2001046408A3 (en) 2001-12-13
JP4627950B2 (ja) 2011-02-09
ES2420842T3 (es) 2013-08-27
EP2128256A2 (en) 2009-12-02
AU785133B2 (en) 2006-10-05
WO2001046408A9 (en) 2002-11-07
EP2128256B1 (en) 2013-06-26
AU2592301A (en) 2001-07-03

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