WO2003095605A3 - A chimeric reverse transcriptase and methods for identifying telomerase inhibitors - Google Patents

A chimeric reverse transcriptase and methods for identifying telomerase inhibitors Download PDF

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Publication number
WO2003095605A3
WO2003095605A3 PCT/EP2003/003874 EP0303874W WO03095605A3 WO 2003095605 A3 WO2003095605 A3 WO 2003095605A3 EP 0303874 W EP0303874 W EP 0303874W WO 03095605 A3 WO03095605 A3 WO 03095605A3
Authority
WO
WIPO (PCT)
Prior art keywords
reverse transcriptase
hiv
htert
methods
telomerase inhibitors
Prior art date
Application number
PCT/EP2003/003874
Other languages
French (fr)
Other versions
WO2003095605A2 (en
Inventor
Juergen Moll
Arndt Schnuchel
Pieter Stouten
Original Assignee
Pharmacia Italia Spa
Juergen Moll
Arndt Schnuchel
Pieter Stouten
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Italia Spa, Juergen Moll, Arndt Schnuchel, Pieter Stouten filed Critical Pharmacia Italia Spa
Priority to EP03722473A priority Critical patent/EP1504090A2/en
Priority to JP2004503599A priority patent/JP2005524409A/en
Priority to AU2003229666A priority patent/AU2003229666A1/en
Publication of WO2003095605A2 publication Critical patent/WO2003095605A2/en
Publication of WO2003095605A3 publication Critical patent/WO2003095605A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention provides nucleic acid molecules encoding a chimeric molecule, HIV-RT/hTERT, is based on HIV-1 Reverse Transcriptase (HIV-RT) and human telomerase reverse transcriptase (hTERT) catalytic site. HIV-RT/hTERT polypeptides and assays for screening compounds that bind to HIV-RT/hTERT and/or inhibit the reverse transcriptase activity of hTERT are also disclosed.
PCT/EP2003/003874 2002-05-08 2003-04-14 A chimeric reverse transcriptase and methods for identifying telomerase inhibitors WO2003095605A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP03722473A EP1504090A2 (en) 2002-05-08 2003-04-14 A chimeric reverse transcriptase and methods for identifying telomerase inhibitors
JP2004503599A JP2005524409A (en) 2002-05-08 2003-04-14 Methods for identifying chimeric reverse transcriptase and telomerase inhibitors
AU2003229666A AU2003229666A1 (en) 2002-05-08 2003-04-14 A chimeric reverse transcriptase and methods for identifying telomerase inhibitors

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37882002P 2002-05-08 2002-05-08
US60/378,820 2002-05-08

Publications (2)

Publication Number Publication Date
WO2003095605A2 WO2003095605A2 (en) 2003-11-20
WO2003095605A3 true WO2003095605A3 (en) 2004-02-19

Family

ID=29420443

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/003874 WO2003095605A2 (en) 2002-05-08 2003-04-14 A chimeric reverse transcriptase and methods for identifying telomerase inhibitors

Country Status (4)

Country Link
EP (1) EP1504090A2 (en)
JP (1) JP2005524409A (en)
AU (1) AU2003229666A1 (en)
WO (1) WO2003095605A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2536830T3 (en) 2010-02-16 2019-10-21 Ultimovacs As POLYPEPTIDES

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0185444A2 (en) * 1984-10-10 1986-06-25 Centocor, Inc. Cloning and expression of HTLV-III DNA
WO1990010230A1 (en) * 1989-02-23 1990-09-07 University Of Ottawa Polypeptide having immunological activity for use as diagnostic reagent and/or vaccine
WO2001007661A2 (en) * 1999-07-23 2001-02-01 Gen-Probe Incorporated Polynucleotide amplification method
WO2001046408A2 (en) * 1999-12-23 2001-06-28 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Molecular clones with mutated hiv gag/pol, siv gag and siv env genes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0185444A2 (en) * 1984-10-10 1986-06-25 Centocor, Inc. Cloning and expression of HTLV-III DNA
WO1990010230A1 (en) * 1989-02-23 1990-09-07 University Of Ottawa Polypeptide having immunological activity for use as diagnostic reagent and/or vaccine
WO2001007661A2 (en) * 1999-07-23 2001-02-01 Gen-Probe Incorporated Polynucleotide amplification method
WO2001046408A2 (en) * 1999-12-23 2001-06-28 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Molecular clones with mutated hiv gag/pol, siv gag and siv env genes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BOSOY DIMITRY ET AL: "Functional analysis of conserved residues in the putative "finger" domain of telomerase reverse transcriptase", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 49, 7 December 2001 (2001-12-07), pages 46305 - 46312, XP002264364, ISSN: 0021-9258 *
KAUR H ET AL: "Preparation of monoclonal antibodies against human telomerase", HYBRIDOMA, vol. 20, no. 3, June 2001 (2001-06-01), pages 183 - 188, XP009022718, ISSN: 0272-457X *
MITCHELL JAMES R ET AL: "Human telomerase activation requires two independent interactions between telomerase RNA and telomerase reverse transcriptase", MOLECULAR CELL, vol. 6, no. 2, August 2000 (2000-08-01), pages 361 - 371, XP002264363, ISSN: 1097-2765 *
PENG YUN ET AL: "Analysis of telomerase processivity: Mechanistic similarity to HIV-1 reverse transcriptase and role in telomere maintenance", MOLECULAR CELL, vol. 7, no. 6, June 2001 (2001-06-01), pages 1201 - 1211, XP002264362, ISSN: 1097-2765 *
ZHU HAIYAN ET AL: "The catalytic subunit of telomerase protects neurons against amyloid beta-peptide-induced apoptosis", JOURNAL OF NEUROCHEMISTRY, vol. 75, no. 1, July 2000 (2000-07-01), pages 117 - 124, XP002264365, ISSN: 0022-3042 *

Also Published As

Publication number Publication date
AU2003229666A1 (en) 2003-11-11
EP1504090A2 (en) 2005-02-09
JP2005524409A (en) 2005-08-18
WO2003095605A2 (en) 2003-11-20
AU2003229666A8 (en) 2003-11-11

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