WO2001025779A3 - Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques - Google Patents

Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques Download PDF

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Publication number
WO2001025779A3
WO2001025779A3 PCT/EP2000/009777 EP0009777W WO0125779A3 WO 2001025779 A3 WO2001025779 A3 WO 2001025779A3 EP 0009777 W EP0009777 W EP 0009777W WO 0125779 A3 WO0125779 A3 WO 0125779A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
another
analysing
fluorescent markers
balanced
Prior art date
Application number
PCT/EP2000/009777
Other languages
German (de)
English (en)
Other versions
WO2001025779A2 (fr
Inventor
Wilhelm Ansorge
Josef Stegemann
Robert Ventzki
Christian Schwager
Original Assignee
Europ Lab Molekularbiolog
Wilhelm Ansorge
Josef Stegemann
Robert Ventzki
Christian Schwager
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europ Lab Molekularbiolog, Wilhelm Ansorge, Josef Stegemann, Robert Ventzki, Christian Schwager filed Critical Europ Lab Molekularbiolog
Priority to AU10219/01A priority Critical patent/AU1021901A/en
Publication of WO2001025779A2 publication Critical patent/WO2001025779A2/fr
Publication of WO2001025779A3 publication Critical patent/WO2001025779A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules

Abstract

Dispositif d'électrophorèse (10) pour l'analyse de molécules marquées à l'aide d'un marqueur fluorescent, en particulier de molécules biologiques, qui, selon un aspect de la présente invention, est approprié pour détecter par résolution au moins quatre marqueurs fluorescents différents indépendamment les uns des autres à l'aide d'un champ détecteur attribué, selon la piste d'électrophorèse sur gel correspondante et le marqueur fluorescent correspondant. A cet effet, des filtres spectraux, relativement à leur sélectivité spectrale, les marqueurs fluorescents, relativement à leur sélectivité d'absorption et d'émission spectrale, et les longueurs d'onde des rayons laser (60, 62, 64, 66) utilisés pour l'excitation des marqueurs fluorescents sont accordés les uns par rapport aux autres de manière correspondante.
PCT/EP2000/009777 1999-10-07 2000-10-05 Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques WO2001025779A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU10219/01A AU1021901A (en) 1999-10-07 2000-10-05 Electrophoresis device for analysing labelled molecules, in particular, biological molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19948391.4 1999-10-07
DE1999148391 DE19948391A1 (de) 1999-10-07 1999-10-07 Elektrophorese-Einrichtung zum Analysieren von markierten Molekülen, insbesondere biologischen Molekülen

Publications (2)

Publication Number Publication Date
WO2001025779A2 WO2001025779A2 (fr) 2001-04-12
WO2001025779A3 true WO2001025779A3 (fr) 2002-02-28

Family

ID=7924860

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/009777 WO2001025779A2 (fr) 1999-10-07 2000-10-05 Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques

Country Status (3)

Country Link
AU (1) AU1021901A (fr)
DE (1) DE19948391A1 (fr)
WO (1) WO2001025779A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004015488B4 (de) 2004-03-26 2008-01-31 Lavision Biotec Gmbh Verfahren zum Auslesen durch streifenweises Abtasten von flächigen Objekten mit Substanzen, die Fluoreszenzstrahlung abgeben
DE102005022880B4 (de) * 2005-05-18 2010-12-30 Olympus Soft Imaging Solutions Gmbh Trennung spektral oder farblich überlagerter Bildbeiträge in einem Mehrfarbbild, insbesondere in transmissionsmikroskopischen Mehrfarbbildern
DE102008034008B4 (de) 2008-07-21 2010-07-01 Carl Zeiss Surgical Gmbh Filtersatz zur Beobachtung von Fluoreszenzstrahlung in biologischem Gewebe
DE102008052223A1 (de) * 2008-10-17 2010-04-22 Albert-Ludwigs-Universität Freiburg Lumineszenzscanner sowie Verfahren zur Detektion von Lumineszenz in Halbleiterbauteilen
DE102016118898B4 (de) * 2016-10-05 2022-03-17 Ferdinand-Braun-Institut gGmbH, Leibniz- Institut für Höchstfrequenztechnik Optisches System und Verfahren zur Spektroskopie
CN109270004B (zh) * 2018-10-11 2023-09-12 南京信息工程大学 基于doas和libs技术的大气分析检测系统和检测方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3046729A1 (de) * 1980-12-11 1982-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg Thermostatische platte fuer eine elektrophoreseeinrichtung
EP0251306A1 (fr) * 1986-07-04 1988-01-07 DIAGEN Institut für molekularbiologische Diagnostik GmbH Procédé et dispositif pour la préparation d'un gradient de température réglable et reproduisable ainsi que son application
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
DE19500638A1 (de) * 1994-01-14 1995-07-20 Hitachi Ltd Elektrophoresegerät
WO1996012948A1 (fr) * 1994-10-20 1996-05-02 Pharmacia Biotech Ab Procede et agencement destines a un dispositif d'electrophorese sur gel
EP0723149A2 (fr) * 1995-01-19 1996-07-24 Hitachi, Ltd. Système d'électrophorèse à réseau de capillaires
WO1996023213A1 (fr) * 1995-01-23 1996-08-01 Murray Anthony J Analyse de molecules biologiques
EP0911630A1 (fr) * 1997-08-07 1999-04-28 Hitachi Electronics Engineering Co., Ltd. Détecteur de fluorescence

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3046729A1 (de) * 1980-12-11 1982-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg Thermostatische platte fuer eine elektrophoreseeinrichtung
EP0251306A1 (fr) * 1986-07-04 1988-01-07 DIAGEN Institut für molekularbiologische Diagnostik GmbH Procédé et dispositif pour la préparation d'un gradient de température réglable et reproduisable ainsi que son application
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
DE19500638A1 (de) * 1994-01-14 1995-07-20 Hitachi Ltd Elektrophoresegerät
WO1996012948A1 (fr) * 1994-10-20 1996-05-02 Pharmacia Biotech Ab Procede et agencement destines a un dispositif d'electrophorese sur gel
EP0723149A2 (fr) * 1995-01-19 1996-07-24 Hitachi, Ltd. Système d'électrophorèse à réseau de capillaires
WO1996023213A1 (fr) * 1995-01-23 1996-08-01 Murray Anthony J Analyse de molecules biologiques
EP0911630A1 (fr) * 1997-08-07 1999-04-28 Hitachi Electronics Engineering Co., Ltd. Détecteur de fluorescence

Also Published As

Publication number Publication date
DE19948391A1 (de) 2001-04-12
WO2001025779A2 (fr) 2001-04-12
AU1021901A (en) 2001-05-10

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