WO2001000805A3 - Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport - Google Patents

Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport Download PDF

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Publication number
WO2001000805A3
WO2001000805A3 PCT/IB2000/000926 IB0000926W WO0100805A3 WO 2001000805 A3 WO2001000805 A3 WO 2001000805A3 IB 0000926 W IB0000926 W IB 0000926W WO 0100805 A3 WO0100805 A3 WO 0100805A3
Authority
WO
WIPO (PCT)
Prior art keywords
mct
membrane
nucleic acid
acid molecules
corynebacterium glutamicum
Prior art date
Application number
PCT/IB2000/000926
Other languages
French (fr)
Other versions
WO2001000805A2 (en
Inventor
Markus Pompejus
Burkhard Kroeger
Hartwig Schroeder
Oskar Zelder
Gregor Haberhauer
Original Assignee
Basf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag filed Critical Basf Ag
Priority to BR0011810-9A priority Critical patent/BR0011810A/en
Priority to CA002380863A priority patent/CA2380863A1/en
Priority to SK1891-2001A priority patent/SK18912001A3/en
Priority to ES00939001T priority patent/ES2177476T1/en
Priority to MXPA01012845A priority patent/MXPA01012845A/en
Priority to AU54216/00A priority patent/AU783707B2/en
Priority to EP00939001A priority patent/EP1255839A2/en
Priority to JP2001506799A priority patent/JP2004513603A/en
Publication of WO2001000805A2 publication Critical patent/WO2001000805A2/en
Publication of WO2001000805A3 publication Critical patent/WO2001000805A3/en
Priority to AU2006200795A priority patent/AU2006200795A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Isolated nucleic acid molecules, designated MCT nucleic acid molecules, which encode novel MCT proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MCT nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated MCT proteins, mutated MCT proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of MCT genes in this organism.
PCT/IB2000/000926 1999-06-25 2000-06-23 Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport WO2001000805A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
BR0011810-9A BR0011810A (en) 1999-06-25 2000-06-23 Isolated nucleic acid molecule, vector, host cell, polypeptide and methods for producing a polypeptide and a fine chemical and for diagnosing the presence or activity of corynebacterium glutamicum in a patient
CA002380863A CA2380863A1 (en) 1999-06-25 2000-06-23 Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
SK1891-2001A SK18912001A3 (en) 1999-06-25 2000-06-23 Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
ES00939001T ES2177476T1 (en) 1999-06-25 2000-06-23 CORYNEBACTERIUM GLUTAMICUM GENES THAT CODIFY PROTEINS INVOLVED IN THE SYNTHESIS OF MEMBRANE AND TRANSPORTATION THROUGH MEMBRANE.
MXPA01012845A MXPA01012845A (en) 1999-06-25 2000-06-23 Corynebacterium glutamicum.
AU54216/00A AU783707B2 (en) 1999-06-25 2000-06-23 Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
EP00939001A EP1255839A2 (en) 1999-06-25 2000-06-23 Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
JP2001506799A JP2004513603A (en) 1999-06-25 2000-06-23 Corynebacterium glutamicum gene encoding a protein involved in membrane synthesis and transport
AU2006200795A AU2006200795A1 (en) 1999-06-25 2006-02-24 Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport

Applications Claiming Priority (70)

Application Number Priority Date Filing Date Title
US14103199P 1999-06-25 1999-06-25
US60/141,031 1999-06-25
DE19931478 1999-07-08
DE19931563 1999-07-08
DE19931478.0 1999-07-08
DE19931563.9 1999-07-08
DE19931454 1999-07-08
DE19931454.3 1999-07-08
DE19932190.6 1999-07-09
DE19932209 1999-07-09
DE19932209.0 1999-07-09
DE19932191.4 1999-07-09
DE19932230 1999-07-09
DE19932229.5 1999-07-09
DE19932227.9 1999-07-09
DE19932125 1999-07-09
DE19932128 1999-07-09
DE19932124.8 1999-07-09
DE19932124 1999-07-09
DE19932180.9 1999-07-09
DE19932128.0 1999-07-09
DE19932212.0 1999-07-09
DE19932230.9 1999-07-09
DE19932191 1999-07-09
DE19932180 1999-07-09
DE19932227 1999-07-09
DE19932212 1999-07-09
DE19932122.1 1999-07-09
DE19932182 1999-07-09
DE19932125.6 1999-07-09
DE19932122 1999-07-09
DE19932190 1999-07-09
DE19932182.5 1999-07-09
DE19932228 1999-07-09
DE19932228.7 1999-07-09
DE19932229 1999-07-09
DE19933005 1999-07-14
DE19933006.9 1999-07-14
DE19932927 1999-07-14
DE19933005.0 1999-07-14
DE19932927.3 1999-07-14
DE19933006 1999-07-14
DE19940764 1999-08-27
DE19940833 1999-08-27
DE19940830.0 1999-08-27
DE19940765 1999-08-27
DE19940831 1999-08-27
DE19940831.9 1999-08-27
DE19940830 1999-08-27
DE19940766.5 1999-08-27
DE19940766 1999-08-27
DE19940833.5 1999-08-27
DE19940765.7 1999-08-27
DE19940764.9 1999-08-27
DE19940832 1999-08-27
DE19940832.7 1999-08-27
DE19941395 1999-08-31
DE19941378.9 1999-08-31
DE19941395.9 1999-08-31
DE19941379 1999-08-31
DE19941379.7 1999-08-31
DE19941378 1999-08-31
DE19942078.5 1999-09-03
DE19942088 1999-09-03
DE19942079 1999-09-03
DE19942077.7 1999-09-03
DE19942077 1999-09-03
DE19942079.3 1999-09-03
DE19942078 1999-09-03
DE19942088.2 1999-09-03

Publications (2)

Publication Number Publication Date
WO2001000805A2 WO2001000805A2 (en) 2001-01-04
WO2001000805A3 true WO2001000805A3 (en) 2001-10-25

Family

ID=27586932

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2000/000926 WO2001000805A2 (en) 1999-06-25 2000-06-23 Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport

Country Status (10)

Country Link
EP (1) EP1255839A2 (en)
JP (2) JP2004513603A (en)
KR (3) KR100834989B1 (en)
CA (1) CA2380863A1 (en)
CZ (1) CZ20014660A3 (en)
ES (1) ES2177476T1 (en)
MX (1) MXPA01012845A (en)
SK (1) SK18912001A3 (en)
TR (4) TR200103708T2 (en)
WO (1) WO2001000805A2 (en)

Families Citing this family (29)

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CN1298854C (en) 1999-07-02 2007-02-07 味之素株式会社 DNA encoding sucrose PTS enzyme II
DE19941478A1 (en) 1999-09-01 2001-03-08 Degussa New nucleotide sequences coding for the thrE gene and process for the fermentative production of L-threonine with coryneform bacteria
US6562607B2 (en) 2000-05-04 2003-05-13 Degussa-Huls Aktiengesellschaft Nucleotide sequences coding for the cls gene
DE10021829A1 (en) * 2000-05-04 2001-11-08 Degussa New nucleotide sequences coding for the pgsA2 gene
DE10021826A1 (en) * 2000-05-04 2001-11-08 Degussa New mutant coryneform bacterium, useful for production of amino acids, especially glutamate, has increased activity of cardiolipin synthase
DE10021828A1 (en) * 2000-05-04 2001-11-08 Degussa New nucleotide sequences coding for the cdsA gene
DE10021831A1 (en) * 2000-05-04 2001-11-08 Degussa New nucleotide sequences coding for the fadD15 gene
DE10021832A1 (en) * 2000-05-04 2001-11-08 Degussa New nucleotide sequences coding for the cma gene
DE10023400A1 (en) 2000-05-12 2001-11-15 Degussa New acyl-carrier protein gene, acp, from coryneform bacteria, useful for increasing fermentative production of amino acids, especially lysine
US20020081673A1 (en) * 2000-06-02 2002-06-27 Bettina Mockel Novel nucleotide sequences coding for the glbO gene
DE10032173A1 (en) * 2000-07-01 2002-01-17 Degussa New nucleotide sequences encoding the plsC gene
DE10045579A1 (en) * 2000-09-15 2002-04-11 Degussa New nucleotide sequences coding for the atr61 gene
AU2001293731A1 (en) * 2000-09-19 2002-04-02 Degussa A.G. Nucleotide sequences coding for the dcta gene
DE10057801A1 (en) * 2000-11-22 2002-05-23 Degussa New polynucleotide encoding the CysQ transporter of coryneform bacteria, useful, when over expressed, for increasing fermentative production of amino acids
DE10154179A1 (en) * 2001-11-05 2003-05-08 Basf Ag Genes coding for membrane synthesis and membrane transport proteins
DE10154270A1 (en) 2001-11-05 2003-05-15 Basf Ag Genes that code for carbon metabolism and energy production proteins
DE10163167A1 (en) * 2001-12-21 2003-07-03 Degussa Process for the fermentative production of L-amino acids and use of coryneform bacteria
EP2243837A1 (en) * 2002-05-14 2010-10-27 Martek Biosciences Corporation Carotene synthase gene and uses therefor
US7468262B2 (en) 2003-05-16 2008-12-23 Ajinomoto Co., Inc. Polynucleotides encoding useful polypeptides in corynebacterium glutamicum ssp. lactofermentum
DE102004009454A1 (en) * 2004-02-27 2005-09-15 Degussa Ag Fermentative production of L-amino acids, especially methionine, useful e.g. in animal nutrition, by culturing bacteria in which components of the methionine uptake system are suppressed
WO2008126896A1 (en) * 2007-04-10 2008-10-23 Ajinomoto Co., Inc. Method for production of organic acid
KR101694850B1 (en) * 2014-10-08 2017-01-10 씨제이제일제당 주식회사 Microorganism producing L-glutamine and process for preparing L-glutamine using the same
WO2018187324A1 (en) * 2017-04-04 2018-10-11 NNB Nutrition USA, LLC Preparation of (r)-3-hydroxybutyric acid or its salts by one-step fermentation
KR101968317B1 (en) * 2018-02-23 2019-04-11 씨제이제일제당 주식회사 Novel L-tryptophan export protein and the method of producing L-tryptophan usingthe same
WO2019228937A1 (en) * 2018-05-28 2019-12-05 Universitaet Des Saarlandes Means and methods for the production of glutarate
EP3670525A1 (en) * 2018-12-18 2020-06-24 Evonik Operations GmbH Method for the fermentative production of l-lysine using c. glutamicum strains with a mutated kup transporter
CN112143676B (en) * 2020-09-21 2022-03-18 山西大学 Bacillus halodurans for producing alkaline protease and method and application of bacillus halodurans for producing alkaline protease
CN114763372A (en) * 2021-01-13 2022-07-19 中国科学院天津工业生物技术研究所 Protein with L-proline efflux function and application thereof
KR102261851B1 (en) * 2021-01-15 2021-06-04 씨제이제일제당 (주) Novel ABC transporter ATP-binding protein variant and a method for producing L-lysine using the same

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EP0252558A2 (en) * 1986-07-04 1988-01-13 SCLAVO S.p.A. Element of dna of corynebacterium diphteriae with typical properties of an is insertion element
EP0752472A1 (en) * 1994-02-24 1997-01-08 Ajinomoto Co., Inc. Novel gene originating in corynebacterium and use thereof
EP0786519A2 (en) * 1996-01-05 1997-07-30 Human Genome Sciences, Inc. Staphylococcus aureus polynucleotides and sequences

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JP2967996B2 (en) * 1989-06-06 1999-10-25 協和醗酵工業株式会社 Method for producing L-tryptophan

Patent Citations (3)

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EP0252558A2 (en) * 1986-07-04 1988-01-13 SCLAVO S.p.A. Element of dna of corynebacterium diphteriae with typical properties of an is insertion element
EP0752472A1 (en) * 1994-02-24 1997-01-08 Ajinomoto Co., Inc. Novel gene originating in corynebacterium and use thereof
EP0786519A2 (en) * 1996-01-05 1997-07-30 Human Genome Sciences, Inc. Staphylococcus aureus polynucleotides and sequences

Non-Patent Citations (3)

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EGGELING L ET AL: "Transport mutants and transport genes of Corynebacterium glutamicum.", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 782, 1996, pages 191 - 201, XP000971888, ISBN: 0-89766-962-2 *
SIEWE RUTH M ET AL: "Functional and genetic characterization of the (methyl)ammonium uptake carrier of Corynebacterium glutamicum.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 10, 1996, pages 5398 - 5403, XP002157003, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
JP2006271389A (en) 2006-10-12
TR200103708T2 (en) 2002-08-21
TR200607443T1 (en) 2007-04-24
KR100834989B1 (en) 2008-06-03
TR200607443T2 (en) 2007-10-22
KR20030002295A (en) 2003-01-08
TR200607442T2 (en) 2007-02-21
TR200607441T2 (en) 2007-02-21
CA2380863A1 (en) 2001-01-04
JP2004513603A (en) 2004-05-13
KR20070087032A (en) 2007-08-27
CZ20014660A3 (en) 2002-09-11
SK18912001A3 (en) 2002-10-08
MXPA01012845A (en) 2002-07-09
EP1255839A2 (en) 2002-11-13
KR100878332B1 (en) 2009-01-14
WO2001000805A2 (en) 2001-01-04
KR20060118626A (en) 2006-11-23
ES2177476T1 (en) 2002-12-16

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