WO2000075657A3 - Screening von target-ligand-wechselwirkungen - Google Patents

Screening von target-ligand-wechselwirkungen Download PDF

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Publication number
WO2000075657A3
WO2000075657A3 PCT/EP2000/004948 EP0004948W WO0075657A3 WO 2000075657 A3 WO2000075657 A3 WO 2000075657A3 EP 0004948 W EP0004948 W EP 0004948W WO 0075657 A3 WO0075657 A3 WO 0075657A3
Authority
WO
WIPO (PCT)
Prior art keywords
chemical library
ligand
ligands
solid phase
target
Prior art date
Application number
PCT/EP2000/004948
Other languages
English (en)
French (fr)
Other versions
WO2000075657A2 (de
Inventor
Richard Ansell
Stefan Seeger
Original Assignee
Molecular Machines & Ind Gmbh
Richard Ansell
Stefan Seeger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Machines & Ind Gmbh, Richard Ansell, Stefan Seeger filed Critical Molecular Machines & Ind Gmbh
Priority to JP2001501881A priority Critical patent/JP2003501660A/ja
Priority to EP00931274A priority patent/EP1194780A2/de
Publication of WO2000075657A2 publication Critical patent/WO2000075657A2/de
Publication of WO2000075657A3 publication Critical patent/WO2000075657A3/de
Priority to US09/996,571 priority patent/US20020106692A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zum Screening von Target-Ligand-Wechselwirkungen unter Verwendung einer chemischen Bibliothek von Liganden, die chemische Bibliothek von Liganden als solche, ein Verfahren zur Herstellung der chemischen Bibliothek sowie die Verwendung der chemischen Bibliothek für die Wirkstoffentwicklung und die Entwicklung molekularer Sensoren. Das Screening-Verfahren umfasst die Schritte: (a) Messung zumindest einer Fluoreszenzeigenschaft einer an einer Festphase immobilisierten, ortsadressierbaren chemischen Bibliothek von Liganden, wobei an jeden Liganden ein molekularer Fluoreszenzsensor gebunden ist, (b) Zugabe des Targets, und (c) Messung der gleichen Fluoreszenzeigenschaft(en) der chemischen Bibliothek wie in Schritt (a). Die an einer Festphase immobilisierte, ortsadressierbare chemische Bibliothek von Liganden ist dadurch gekennzeichnet, dass jeder Ligand an einen molekularen Fluoreszenzsensor gebunden ist, vorzugsweise derart, dass der molekulare Fluoreszenzsensor zwischen dem Liganden und der Festphase und/oder an dem der Festphase gegenüberliegenden Ende des Liganden gebunden ist.
PCT/EP2000/004948 1999-06-02 2000-05-30 Screening von target-ligand-wechselwirkungen WO2000075657A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2001501881A JP2003501660A (ja) 1999-06-02 2000-05-30 ターゲット−リガンド相互作用のスクリーニング
EP00931274A EP1194780A2 (de) 1999-06-02 2000-05-30 Screening von target-ligand-wechselwirkungen
US09/996,571 US20020106692A1 (en) 1999-06-02 2001-11-30 Screening of target-ligand interactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19925402A DE19925402C2 (de) 1999-06-02 1999-06-02 Screening von Target-Ligand-Wechselwirkungen
DE19925402.8 1999-06-02

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/996,571 Continuation US20020106692A1 (en) 1999-06-02 2001-11-30 Screening of target-ligand interactions

Publications (2)

Publication Number Publication Date
WO2000075657A2 WO2000075657A2 (de) 2000-12-14
WO2000075657A3 true WO2000075657A3 (de) 2001-09-27

Family

ID=7910105

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/004948 WO2000075657A2 (de) 1999-06-02 2000-05-30 Screening von target-ligand-wechselwirkungen

Country Status (5)

Country Link
US (1) US20020106692A1 (de)
EP (1) EP1194780A2 (de)
JP (1) JP2003501660A (de)
DE (1) DE19925402C2 (de)
WO (1) WO2000075657A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6019441A (en) * 1997-10-09 2000-02-01 General Motors Corporation Current control method for a solenoid operated fluid control valve of an antilock braking system
DE10109777A1 (de) * 2001-03-01 2002-09-19 Infineon Technologies Ag Verfahren zum Erfassen von makromolekularen Biopolymeren mittels mindestens einer Einheit zum Immobilisieren von makromolekularen Biopolymeren
DE10109779A1 (de) * 2001-03-01 2002-09-19 Infineon Technologies Ag Vorrichtung und Verfahren zum Erfassen von makromolekularen Biopolymeren mittels mindestens einer Einheit zum Immobilisieren von makromolekularen Biopolymeren
DE10314579A1 (de) * 2003-03-31 2004-10-14 Carl Zeiss Jena Gmbh Verfahren und Vorrichtung zur Sequenzanalyse von Nukleinsäuren
WO2005043137A1 (de) * 2003-10-18 2005-05-12 Bayer Healthcare Ag Direkte beobachtung molekularer veränderungen in biologischen testsystemen mittels messungen der fluoreszenz-lebensdauer
DE102004050032A1 (de) * 2004-10-13 2006-04-27 Micronas Gmbh Verfahren zum Nachweisen und/oder zum Bestimmen der Konzentration mindestens eines Liganden
CN100483112C (zh) * 2006-05-19 2009-04-29 湖南大学 一种检测有机溶剂中水含量的荧光化学传感器及其应用
JP5219144B2 (ja) * 2007-12-28 2013-06-26 公益財団法人新産業創造研究機構 新規アフィニティーラベル化方法及びラベル化方法を用いたスクリーニング方法
KR101847264B1 (ko) * 2016-03-29 2018-04-10 국립암센터 항원 반응형 항체-형광염료 결합체 및 이를 이용한 표적 세포의 형광영상 검출방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040065A2 (en) * 1996-04-24 1997-10-30 Peptide Therapeutics Limited Substrates and inhibitors of proteolytic enzymes
US5786219A (en) * 1996-10-28 1998-07-28 Molecular Probes, Inc. Microspheres with fluorescent spherical zones
WO1998053093A1 (en) * 1997-05-23 1998-11-26 Bioarray Solutions Llc Color-encoding and in-situ interrogation of matrix-coupled chemical compounds
WO1999011655A1 (en) * 1997-09-04 1999-03-11 Gryphon Sciences Modular protein libraries and methods of preparation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215641D0 (en) * 1992-07-23 1992-09-09 Sharma Ashutosh Nadh detection
US5837475A (en) * 1997-01-30 1998-11-17 Hewlett-Packard Co. Apparatus and method for scanning a chemical array

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040065A2 (en) * 1996-04-24 1997-10-30 Peptide Therapeutics Limited Substrates and inhibitors of proteolytic enzymes
US5786219A (en) * 1996-10-28 1998-07-28 Molecular Probes, Inc. Microspheres with fluorescent spherical zones
WO1998053093A1 (en) * 1997-05-23 1998-11-26 Bioarray Solutions Llc Color-encoding and in-situ interrogation of matrix-coupled chemical compounds
WO1999011655A1 (en) * 1997-09-04 1999-03-11 Gryphon Sciences Modular protein libraries and methods of preparation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GORDON E M ET AL: "APPLICATIONS OF COMBINATORIAL TECHNOLOGIES TO DRUG DISCOVERY. 2. COMBINATORIAL ORGANIC SYNTHESIS, LIBRARY SCREENING STRATEGIES, AND FUTURE DIRECTIONS", JOURNAL OF MEDICINAL CHEMISTRY,AMERICAN CHEMICAL SOCIETY. WASHINGTON,US, vol. 37, no. 10, 13 May 1994 (1994-05-13), pages 1384 - 1401, XP000195953, ISSN: 0022-2623 *
RANO ET AL: "A combinatorial approach for determining protease specificities: application to intereukin-1beta converting enzyme (ICE)", CHEM. BIOL., vol. 4, 1997, pages 149 - 55, XP000995163 *

Also Published As

Publication number Publication date
US20020106692A1 (en) 2002-08-08
EP1194780A2 (de) 2002-04-10
JP2003501660A (ja) 2003-01-14
DE19925402C2 (de) 2001-12-20
WO2000075657A2 (de) 2000-12-14
DE19925402A1 (de) 2000-12-14

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