WO2000071425A1 - Procede et dispositif d'ouverture de recipients fermes par un film d'etancheite - Google Patents

Procede et dispositif d'ouverture de recipients fermes par un film d'etancheite Download PDF

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Publication number
WO2000071425A1
WO2000071425A1 PCT/EP2000/004513 EP0004513W WO0071425A1 WO 2000071425 A1 WO2000071425 A1 WO 2000071425A1 EP 0004513 W EP0004513 W EP 0004513W WO 0071425 A1 WO0071425 A1 WO 0071425A1
Authority
WO
WIPO (PCT)
Prior art keywords
film
chamber
heat source
heating
sealed
Prior art date
Application number
PCT/EP2000/004513
Other languages
German (de)
English (en)
Inventor
Igor Ivanov
Original Assignee
Gpc Biotech Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gpc Biotech Ag filed Critical Gpc Biotech Ag
Priority to AU53952/00A priority Critical patent/AU5395200A/en
Priority to EP00938653A priority patent/EP1183185A1/fr
Publication of WO2000071425A1 publication Critical patent/WO2000071425A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
    • B65B69/00—Unpacking of articles or materials, not otherwise provided for

Definitions

  • the present invention relates to a method and a device for opening containers sealed with a sealing film.
  • thermoplastic material usually in the form of a thin film, with which the object to be sealed is sealed or closed so that two surfaces made of thermoplastic material come into contact at all remaining openings. These are then briefly heated above the melting point of the thermoplastic material in such a way that the surfaces fuse at the heated points and a sealed, continuous container or a hermetic seal results (see, for example, US 3765990, US 3648428, US 3792567, US 4001075).
  • Thermoplastic sealing is also used, for example, in micro- and molecular biology or genetics on microtiter plates, partly for storing samples at low temperatures or for preventing water ingress when polymerase chain reactions (PCR) are carried out in parallel on a large scale which the microplates are immersed in water baths.
  • Devices for heat sealing microtiter plates are offered, for example, by Advanced Biotechnologies, Epsom, UK or Genetix Ltd., Wales, UK.
  • a permanent seal is not always the goal.
  • the seal is intended to protect the goods, for example, from damage during transport or from the entry or exit of water, oxygen or microorganisms during transport and storage or sterilization (cf. US 5679392), but must be removed before the goods are used again become. While the seal can be removed from insensitive goods by tearing or cutting the thermoplastic material (eg WO 98/52861, US 5613346), such methods cannot be used in all cases.
  • Another proposal is to briefly reheat the plates sealed with polypropylene film after use in the same device used for the sealing (temperature approx. 170 ° C.) and then to remove the film.
  • the microtiter plates need to be sealed at 170 ° C for approx. 3-4 s (Q-Sealer Heat Sealing Machine: Users Guide, Genetix Ltd.).
  • Q-Sealer Heat Sealing Machine Users Guide, Genetix Ltd.
  • the hot seal must be removed from the microtiter plate after heating within such a short time that many users tend to tear quickly. This in turn can lead to sample loss and cross-contamination.
  • microtapes TW Astle; MicroTape - A 384-Well Ultra High Throughput Screening System; LabAutomation '99, Final Conference Program, p. 122; Association for Laboratory Automation, Charlottesville, USA; 1999.
  • These are strips of an elastic film which have been embossed in such a way that cavities similar to a microtiter plate are made available, but which, after filling and sealing, can be rolled up for storage. They are particularly suitable for space-saving storage of a large number of samples as well as for a continuous working procedure when sampling by unrolling the tape. Especially with a continuous way of working but the problem, the seal as shortly before the
  • microtapes originally came from the electronics industry, where they were used for packaging components.
  • Badarf provides an inexpensive, fast method for unpacking the components, which does not endanger the packaged goods, and a device for carrying it out.
  • the method according to the invention and the device according to the invention can also be used here.
  • the present invention has for its object to provide a method and an apparatus for opening containers closed with a film.
  • the invention provides a method for removing seals which have been produced in particular using thermoplastic materials by means of the method of heat sealing, the seal being burst open and / or at least partially by means of a heat source which is arranged at a distance from the seal burns, making the sealed goods accessible. Furthermore, the invention relates to a device which is suitable for carrying out such a method.
  • the invention enables simple and gentle opening or removal of seals.
  • the film and an underlying gas layer in the container by briefly approaching a heat source strongly heated.
  • the temperature of the heat source and the duration of the action are preferably chosen so that
  • the duration of exposure and the temperature of the heat source must also be matched to the thermoplastic material of the seal.
  • the gases generated during the partial combustion of the thermoplastic material can be removed in a gas stream. It is advantageous if the temperature of the heat source is above the ignition point of the thermoplastic material, so that the residues remaining after the seal has burst open at least partially burn off or melt away and expose the cavity.
  • Films with particularly good absorption properties for the emitted energy are preferably used.
  • a dark-colored polypropylene film absorbs energy in the frequency spectrum of the visible light better than a non-colored film.
  • the container and preferably the material contained therein is cooled. It becomes the bottom of the container or the container is completely cooled.
  • the cooling takes place with a cooling device or in which the container is placed on dry ice.
  • the invention has several advantages over the previously used methods.
  • seals can be removed much faster and more reliably than with the methods previously used.
  • the sealed material is further heated significantly less in the embodiment according to the invention and is therefore protected. Since no further manual removal of the seal is necessary, this process is simpler and more user-friendly, while at the same time minimizing the risk of loss of sealed goods and the undesired entry of foreign bodies or substances.
  • it offers the possibility of working under sterile conditions, especially in the described applications in microbiology and molecular biology or genetics.
  • the invention enables a new method for storing biological material. Above all, it avoids cross-contamination of the samples contained in a microtiter plate or microtape.
  • the high temperature of the top layer when heat sealed leads to sterilization.
  • the time when sealing and opening the film is so short that the samples contained are not heated.
  • the microtiter plate or microtape is cooled, only the top layer of the microtiter plate or microtape is heated by the hot gas stream. It opens in a few seconds.
  • the heat transfer that occurs both from plastic and from water-based solutions is low.
  • the three factors mentioned above open up the possibility of using polypropylene plates for storing biological material such as bacteria, viruses, cells, compounds, etc.
  • Another possible application is to use heat-sealed microtiter plates or microtapes for the lysis of bacteria or yeast by heating.
  • An advantageous use is for further analysis, e.g. B. by PCR.
  • the method can also be used to open plates in which banks of connections are stored. So far, the main problem of contamination has been that which can be avoided with the present invention.
  • An additional advantage is the use of DMSO (dimethyl sulfoxide), a chemical compound that is used to store biological material.
  • DMSO dimethyl sulfoxide
  • the melting point of DMSO is about 12 ° C. This facilitates the handling of solutions and mixtures that are to be kept frozen or liquid.
  • Microtiter plates with 96 wells are usually used to store connections.
  • the present invention enables the handling of microtiter plates that have a higher number of wells.
  • the method of opening microtiter plates using a gas burner opens up the possibility of storing compounds in microtiter plates which have 384 or more wells up to 1536 wells.
  • the method is also suitable for opening the seal in microtapes for transport, storage and use of biological samples as well as electronic components.
  • a device according to the invention can be integrated into the process in such a way that exactly the number of sample chambers / component chambers are opened which are sampled in the next sampling cycle. This, and the high speed when removing the seal, in particular Especially with biological and chemical samples, material losses due to evaporation are minimized and variances between samples due to different times between removal of the seal and sampling avoided. Electronic components can be removed quickly and without endangering the components, for example due to the edges of cutting tools.
  • the method according to the invention is also particularly suitable for discontinuous work with microtapes, since it makes it possible to remove the sealing of those sample chambers / component chambers from which material is to be removed, specifically and individually, regardless of their position on the belt.
  • the method is carried out as follows.
  • samples are placed in the wells of a microtiter plate or microtape.
  • the wells in the microtiter plate or microtape are sealed with a sealing film, for example by heat sealing.
  • the microtiter plate or microtape with the samples is transported to another location and stored there, for example at cool temperatures.
  • one or more of the plurality of wells in the microtiter plate or microtape are exposed by opening the corresponding seal using the method described above.
  • one or two or more rows of the rows on one of the microtiter plates or on a microtape can be opened in this fourth step.
  • a fifth step the samples are taken from the exposed wells, for example for a subsequent examination. Then steps 3 - 5 can be repeated one or more times. This means that the remaining samples can be stored again and, in a further step, some or more of the samples exposed from individual wells or from one or more rows for a subsequent examination. For example, two rows of a microtiter plate, which has a total of 12 rows, can be opened in six successive processes.
  • the system which can be sealed with a sealing film and has a large number of recesses, enables the use of automated systems.
  • Heat sealing herein can also be understood to mean the contactless heating of the sealing film by radiation.
  • a sealing film can be applied to seal a recess in a microtiter plate or a microtape by gluing.
  • the opening or exposure of the recess is carried out in the same way as described above.
  • the present system is not restricted to a specific material, in particular the microtiter plates or microtapes can consist of plastic such as polypropylene, polycarbonate and polystyrene.
  • the sealing films can be made of plastic, e.g. B. made of polypropylene, polystyrene or polyethylene or EVA. Multi-layer films and laminates can also be used, e.g. metal coated foils, e.g. foils coated with aluminum.
  • the present invention enables the use of systems in which the recess is closed with a film, e.g. Polypropylene sheets for storing biological and chemical samples.
  • the biological samples can be eukaryotic or procariotic cells, viruses, proteins or nucleic acids (genomes, RNA, cDNA, PCR fragments).
  • the present invention has the particular advantage that the individual samples can be safely stored in a microtiter plate or microtape and when individual contamination of the samples with one another is reliably avoided.
  • FIGS 1A, B and C plan views of microtiter plates after the
  • FIGS. 2A, B, C and D are top views of microtiter plates from which the sealing film has been at least partially removed, the sealing being carried out according to a known method by heating by means of a heating plate after different heating times, and
  • FIGS. 3A and B show the top view of a microtiter plate with a seal or after the seals have been opened using the method according to the invention
  • Figure 4 is a side view of a preferred embodiment of a device according to the invention for opening seals and
  • Figure 5 is a front view of the embodiment of Figure 4.
  • Polypropylene PP microtiter plates with 384 wells (Cat. # X5005, Genetix) and polypropylene film (Heat Seal Film, cat # X5151, Genetix) were made using a heat sealing device from Genetix (Cat. # X5161), consisting of a heat-dissipating base plate and a electrically heated top plate, to which a handle is attached for exerting pressure on the surface to be sealed, sealed with a film.
  • the heat sealing device was preheated for at least 15 minutes and at a temperature of
  • the plates sealed in this way were immersed three times in a water bath at 85 ° C. for 10 minutes and immediately cooled in an ice bath for 5 minutes. Finally, the plates were washed under running water, transferred to a transilluminator and evaluated using a Herolab E.A.S.Y system.
  • Cresol red is a pH indicator and changes color from an intense red at pH> 8.8 to pale yellow at pH ⁇ 7.2.
  • the quality of the seal could therefore be recognized by the disappearance of the red color in the wells of the microtiter plate.
  • intactly sealed depressions can be recognized as dark points, while light or colorless points represent depressions whose contents could be washed out due to an incomplete seal or a breach of the seal.
  • FIGS. 1A, B and C show typical results for the sealing periods of 2x1 s, 2x2 s and 2x4 s used. As Figure IC shows, only the plates heated for 2x4 s gave a reliably tight seal sealing. After heating for 2x1 s, 15 + 8 wells per plate and after 2x2 s 5 ⁇ 6 wells remained unsealed.
  • the plates were placed in the sealing device, three plates were heated under pressure as described above for 10, 20, 40 or 80 s each, and the seal was then removed in one go. After removing the seal, the plates were washed under running water, transferred to a transilluminator and evaluated using a Herolab E.A.S.Y system.
  • FIGS. 2A, B, C and D show typical examples of the results obtained with the heating times examined. Only when the plates are 40 s (Fig. 2C) or preferably 80 s (Fig. 2D) were heated, the seal was reliably removed.
  • the seals were removed using a Bunsen burner.
  • the plates were placed on a flat surface and a Bunsen burner was moved over the plate in a uniform movement at a distance of 5-10 cm so that the flame did not sweep over any point on the plate for more than 1 s.
  • the opening of the seal could be observed as bursting and rapid melting and burning of the polypropylene film.
  • the plates were washed under running water, transferred to a transilluminator and evaluated using a Herolab E.A.S.Y system.
  • Figure 3 shows a typical example of the results obtained: Figure 3A shows a microtiter plate after sealing and washing, Figure 3B the same plate after treatment with the Bunsen burner. All wells were reliably opened in less than 3 s.
  • FIGS. 4 and 5 show a basic illustration of a device according to the invention for opening seals on microtiter plates.
  • a microtiter plate 1 is arranged on a conveyor belt 2 and is transported through a housing 5 with the aid of the conveyor belt.
  • a gas burner device 3 is arranged above the conveyor belt and serves as a heat source in this preferred embodiment.
  • a plurality of gas and air supply connections 4 are provided. In the example shown, a total of five gas flames are shown, which are arranged transversely to the transport direction of the conveyor belt and point in the direction of the microtiter plate 1.
  • the heat source 3 is arranged pivotably, as a result of which the direction of the gas flames pointing to the microtiter plate can be adjusted.
  • the angle relative to the perpendicular to the conveyor belt can preferably be pivoted by an angle within a range of +/- 45 °.
  • a hot air gun can be used to direct a hot air stream onto the microtiter plate or microtape using the device 3.
  • the temperature and the rate of heating of the sealing film can be achieved by regulating the gas / air supply accordingly.
  • the distance and / or the angle of the gas burner device to the position of the microtiter plate or the conveyor belt can be set become.
  • the duration of exposure of the heat source to the microtiter plate can be determined by adjusting the transport speed. Another possibility is to set the duration of the heat supply, for example by only supplying the gas / air during a certain heating period.
  • the invention is not limited to the prescribed exemplary embodiment, rather the method and the device according to the invention can be used in a variety of ways for opening containers sealed with foils, wherein different foils or seals can be opened and removed.
  • a hot air gun can also be used, in particular a hot air gun that works at temperatures up to 600 ° C.
  • not all seals are opened at the same time by a microtiter plate or a microtape, but only certain desired depressions are uncovered in each case.
  • the wells of a single row or several rows of such a microtiter plate can be opened in a first process.
  • the samples of the exposed wells can be examined, for example with the aid of an automatic system.
  • the microtiter plate or microtape with the samples remaining therein can then be stored again, for example overnight or for a week or for a long time.
  • storage can take place at low temperatures, for example - 20 ° C to - 70 ° C.
  • the seals are removed from further wells, for example the next or more of the next rows are opened by a microtiter plate or a microtape.
  • the samples can be taken again and transferred to agar, for example.
  • the process, ie storing the remaining Samples and subsequent opening of certain of the existing wells can be repeated several times if necessary.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un procédé pour l'ouverture d'au moins un compartiment fermé par un film, caractérisé par un chauffage de courte durée du film et/ou un gaz se trouvant à l'intérieur du compartiment, de façon que le film soit amené à se déchirer et/ou soit au moins partiellement fondu ou brûlé.
PCT/EP2000/004513 1999-05-19 2000-05-18 Procede et dispositif d'ouverture de recipients fermes par un film d'etancheite WO2000071425A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU53952/00A AU5395200A (en) 1999-05-19 2000-05-18 Method and device for opening containers that have been sealed with a sealing film
EP00938653A EP1183185A1 (fr) 1999-05-19 2000-05-18 Procede et dispositif d'ouverture de recipients fermes par un film d'etancheite

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1999122902 DE19922902A1 (de) 1999-05-19 1999-05-19 Verfahren und Vorrichtung zum Öffnen von mit einer Siegelfolie verschlossenen Behältern
DE19922902.3 1999-05-19

Publications (1)

Publication Number Publication Date
WO2000071425A1 true WO2000071425A1 (fr) 2000-11-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/004513 WO2000071425A1 (fr) 1999-05-19 2000-05-18 Procede et dispositif d'ouverture de recipients fermes par un film d'etancheite

Country Status (4)

Country Link
EP (1) EP1183185A1 (fr)
AU (1) AU5395200A (fr)
DE (1) DE19922902A1 (fr)
WO (1) WO2000071425A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10252572B4 (de) * 2002-11-12 2006-06-01 Bausch + Ströbel Maschinenfabrik Ilshofen GmbH + Co. Verfahren und Vorrichtung zum Entfernen eines die Öffnung eines Spritzen-Magazinkastens verschließenden Abdeckblattes
DE102004003232A1 (de) * 2004-01-22 2005-08-11 Groninger & Co Gmbh Verfahren und Einrichtung zum Entfernen einer Abdeckung von einem Magazinkasten
DE102016007783A1 (de) 2016-06-24 2017-12-28 Giesecke+Devrient Currency Technology Gmbh Verfahren, Vorrichtung und System zum Öffnen von Wertdokumentverpackungen

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3648428A (en) 1968-02-20 1972-03-14 Dow Chemical Co Film-to-film skin packaging
US3765990A (en) 1971-08-12 1973-10-16 Gerrard Co Ltd Apparatus for side sealing two layers of heat sealable film to complete a package
US3792567A (en) 1971-12-07 1974-02-19 Dixie Packaging Co Inc Heat sealing machine
US4001075A (en) 1974-11-20 1977-01-04 Marathon Cheese Corporation Seal for packages capable of thermoplastic sealing and means for effecting such sealing
US5613346A (en) 1993-06-21 1997-03-25 Kirin Beer Kabushiki Kaisha Apparatus for removing packing material from packed article
US5622471A (en) * 1991-11-08 1997-04-22 National Recovery Technologies, Inc. Method and apparatus for opening and emptying bags containing recyclable materials
US5679392A (en) 1995-06-07 1997-10-21 Schegan; John Heat treatment of raw molluscan shellfish
WO1998052861A1 (fr) 1997-05-23 1998-11-26 Deltagraph A/S Appareil et procede permettant d'ouvrir et de vider un contenant, et utilisation de cet appareil

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1511847A1 (de) * 1965-04-23 1970-01-08 Lars Wiking Einrichtung zum OEffnen von Verpackungen aus einem hitzeempfindlichen Material
DE4126854A1 (de) * 1991-06-12 1992-12-17 Franz Edwin Ratzky Verfahren und vorrichtung zum aufbringen eines klebers

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3648428A (en) 1968-02-20 1972-03-14 Dow Chemical Co Film-to-film skin packaging
US3765990A (en) 1971-08-12 1973-10-16 Gerrard Co Ltd Apparatus for side sealing two layers of heat sealable film to complete a package
US3792567A (en) 1971-12-07 1974-02-19 Dixie Packaging Co Inc Heat sealing machine
US4001075A (en) 1974-11-20 1977-01-04 Marathon Cheese Corporation Seal for packages capable of thermoplastic sealing and means for effecting such sealing
US5622471A (en) * 1991-11-08 1997-04-22 National Recovery Technologies, Inc. Method and apparatus for opening and emptying bags containing recyclable materials
US5613346A (en) 1993-06-21 1997-03-25 Kirin Beer Kabushiki Kaisha Apparatus for removing packing material from packed article
US5679392A (en) 1995-06-07 1997-10-21 Schegan; John Heat treatment of raw molluscan shellfish
WO1998052861A1 (fr) 1997-05-23 1998-11-26 Deltagraph A/S Appareil et procede permettant d'ouvrir et de vider un contenant, et utilisation de cet appareil

Also Published As

Publication number Publication date
EP1183185A1 (fr) 2002-03-06
AU5395200A (en) 2000-12-12
DE19922902A1 (de) 2000-11-30

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