WO2000053034A1 - Agent annihilant l'oxygene actif - Google Patents
Agent annihilant l'oxygene actif Download PDFInfo
- Publication number
- WO2000053034A1 WO2000053034A1 PCT/JP1999/001397 JP9901397W WO0053034A1 WO 2000053034 A1 WO2000053034 A1 WO 2000053034A1 JP 9901397 W JP9901397 W JP 9901397W WO 0053034 A1 WO0053034 A1 WO 0053034A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- active oxygen
- syrup
- solvent
- locust bean
- annihilating
- Prior art date
Links
- 239000001301 oxygen Substances 0.000 title claims abstract description 41
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 41
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 40
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 26
- 235000013912 Ceratonia siliqua Nutrition 0.000 claims abstract description 43
- 240000008886 Ceratonia siliqua Species 0.000 claims abstract description 43
- 239000002904 solvent Substances 0.000 claims abstract description 29
- 239000000843 powder Substances 0.000 claims abstract description 23
- 239000006188 syrup Substances 0.000 claims abstract description 23
- 235000020357 syrup Nutrition 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 235000019441 ethanol Nutrition 0.000 claims abstract description 7
- 230000000694 effects Effects 0.000 claims description 18
- 238000002360 preparation method Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 4
- 102000019197 Superoxide Dismutase Human genes 0.000 claims description 2
- 108010012715 Superoxide dismutase Proteins 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 125000005909 ethyl alcohol group Chemical group 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 13
- 239000000463 material Substances 0.000 abstract description 9
- 239000000654 additive Substances 0.000 abstract description 4
- 230000000996 additive effect Effects 0.000 abstract description 4
- 239000002154 agricultural waste Substances 0.000 abstract description 4
- 235000008429 bread Nutrition 0.000 abstract description 3
- 238000001704 evaporation Methods 0.000 abstract description 3
- 235000013402 health food Nutrition 0.000 abstract description 3
- 238000003892 spreading Methods 0.000 abstract description 2
- 230000007480 spreading Effects 0.000 abstract description 2
- 230000001473 noxious effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 13
- 150000001720 carbohydrates Chemical class 0.000 description 11
- 239000000126 substance Substances 0.000 description 9
- 239000013543 active substance Substances 0.000 description 5
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 235000011837 pasties Nutrition 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 3
- DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical compound CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 description 3
- 244000299461 Theobroma cacao Species 0.000 description 3
- 230000002411 adverse Effects 0.000 description 3
- 230000001476 alcoholic effect Effects 0.000 description 3
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- -1 superoxide anions Chemical class 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 238000004435 EPR spectroscopy Methods 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 235000009470 Theobroma cacao Nutrition 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- BTANRVKWQNVYAZ-UHFFFAOYSA-N butan-2-ol Chemical compound CCC(C)O BTANRVKWQNVYAZ-UHFFFAOYSA-N 0.000 description 2
- 229940090949 docosahexaenoic acid Drugs 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 150000005846 sugar alcohols Chemical class 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- DVSZKTAMJJTWFG-SKCDLICFSA-N (2e,4e,6e,8e,10e,12e)-docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C=C\C=C\C=C\C(O)=O DVSZKTAMJJTWFG-SKCDLICFSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- GZJLLYHBALOKEX-UHFFFAOYSA-N 6-Ketone, O18-Me-Ussuriedine Natural products CC=CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O GZJLLYHBALOKEX-UHFFFAOYSA-N 0.000 description 1
- 241000208140 Acer Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229920000161 Locust bean gum Polymers 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 235000005764 Theobroma cacao ssp. cacao Nutrition 0.000 description 1
- 235000005767 Theobroma cacao ssp. sphaerocarpum Nutrition 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000001046 cacaotero Nutrition 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- KAUVQQXNCKESLC-UHFFFAOYSA-N docosahexaenoic acid (DHA) Natural products COC(=O)C(C)NOCC1=CC=CC=C1 KAUVQQXNCKESLC-UHFFFAOYSA-N 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229940035429 isobutyl alcohol Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000711 locust bean gum Substances 0.000 description 1
- 235000010420 locust bean gum Nutrition 0.000 description 1
- 239000000845 maltitol Substances 0.000 description 1
- 235000010449 maltitol Nutrition 0.000 description 1
- VQHSOMBJVWLPSR-WUJBLJFYSA-N maltitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-WUJBLJFYSA-N 0.000 description 1
- 229940035436 maltitol Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 235000012771 pancakes Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000020095 red wine Nutrition 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 235000021101 unsweetened yogurt Nutrition 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/10—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
- A23L29/238—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin from seeds, e.g. locust bean gum or guar gum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- the present invention relates to an active oxygen annihilating agent as a foodstuff additive and a method for the preparation thereof by utilizing an agricultural waste material as well as to a foodstuff containing the active oxygen annihilating agent.
- Active oxygen is a conceptual term which generally means several oxidative species including superoxide anions, oxygen radicals, hydroxyl radicals, hydrogen peroxide and the like having, when taken up from outside into or generated inside of a human body, adverse influences on the health condition of the human body such as peroxidation of lipids and mutation of DNAs to give increasing damages on living cells slowly but steadily disturbing normal activities to maintain the living body and finally leading to formation of malignant tumors and senescence acceleration. Accordingly, it is an important problem to reduce take-up amounts of active oxygen into human body.
- living bodies in general have a mechanism to render the active oxygen innoxious which suppresses appearance of the above mentioned unsound or diseased condition of the living body.
- This suppressing activity against the adverse effects of active oxygen is largely by virtue of the superoxide dismutase, referred to as SOD hereinafter, as a kind of enzymes, of which abundance level in the living body widely differs among individuals depending on the ages, races, living environments and other factors.
- SOD superoxide dismutase
- the overly active oxygen can be annihilated by factitiously supplying a substance which exhibits the same activity as the SOD from outside at least temporarily.
- Such a substance is usually referred to as an SOD-like substance.
- SOD-like substances including polyphenol compounds in red wine and cacao beans and catechms in green tea.
- the content of the SOD-like substance in these foodstuffs is so low that they are practically inapplicable to an acute symptom due to excess of active oxygen requiring an unduly large amount of the foodstuff intake.
- the SOD-like substances in foodstuffs cannot be concentrated without adversely affecting the tastmess or flavo ⁇ ness necessarily to degrade the quality of the foodstuff. Accordingly, the inventors have conducted extensive screening tests of a variety of natural products with an object to uncover a material exhibiting an SOD-like activity to direct their attention to locust bean pods.
- Locust bean is a leguminous plant having a scientific name of Ceratonia siliqua L. and growing in the Mediterranean coastal districts such as Italy, Spain and Portugal.
- the major application of locust beans is as a food additive including a powder obtained by pulverizing the seed albumen of locust beans to serve as a thickening and stabilizing agent called a locust bean gum.
- locust beans production of locust beans is necessarily accompanied by a corresponding amount of locust been pods as an agricultural waste material.
- locust bean pods as a by-product of locust beans, only a very small portion of the annual production of locust bean pods is utilized, for example in the United States, as a cocoa substitute or a coating material on confectionery and almost all of the locust bean pod production is merely discarded or, at best, utilized as a livestock feed.
- value-added utilization of locust bean pods is an important issue heretofore in the agricultural industry.
- the inventors have directed their attention to locust bean pods as one of the raw materials in their screening work to uncover an efficient and inexpensive source material of an SOD-like substance.
- the present invention accordingly provides a novel active oxygen annihilating agent having SOD-like activity which is an extract from locust bean pods in the form of a syrup or powder.
- the invention also provides a foodstuff admixed with the active oxygen annihilating agent.
- the above mentioned active oxygen annihilating agent having SOD-like activity is prepared from locust bean pods by a method which comprises the steps of: (a) crushing locust bean pods into particulates;
- the active oxygen annihilating agent in the form of a syrup or powder prepared in the above described manner contains, in addition to the active oxygen annihilating ingredient, a good amount of saccharides so that the syrup or powder can be added to a foodstuff of any types as a sweetening material besides the effect of active oxygen annihilation .
- the inventors conducted, as an outset, a chemical analysis of locust bean pods to determine various saccharide compounds including monosaccharides, disacchar ides and sugar alcohols.
- the results were as follows, where the saccharide contents per 100 g of dried locust bean pods are given in five ratings with the respective symbols of: (*) for less than 0.05 g; (**) for less than 0.3 g; (+) for 0.05 to 1 g; (++) for 1 to 10 g; and (+++) for more than 10 g.
- fructose (++) glucose ( ++ ) ; arabmose ( + ) ; xylose (+) ; mannose (+ ) ; and galactose (*)
- the saccharide constituent of locust bean pods consists mostly of sucrose, fructose and glucose so that the extract from locust bean pods has little problem on the health of human bodies even when taken in a relatively large amount. It is a quite unexpected discovery that, while absolutely no SOD-like activity can be noted in the germs and seed albumens of locust beans per se, very strong active oxygen annihilating activity is exhibited by the extract from locust bean pods. In the preparation of the inventive active oxygen annihilating agent, locust bean pods are subjected to solvent extraction by using an extractant solvent to give an extract solution.
- the extract solution as such exhibits a certain level of active oxygen annihilating activity
- the syrupy liquid can be freeze-dried or spray-dried to give an active oxygen annihilating agent in the form of a powder.
- locust bean pods are dried and crushed into particulates of a 0.01 to 1.5 mm or, preferably, smaller than 1.0 mm particle diameter.
- the particulates of locust bean pods are then brought into contact with an extractant solvent to effect extraction of the active substances to give an extract solution.
- Water is preferable as the extractant solvent although some organic solvents can be used for the purpose including alcoholic solvents such as methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, n-butyl alcohol, isobutyl alcohol, sec-butyl alcohol and tert-butyl alcohol, acetone, chloroform and n-hexane. These solvents can be used either singly or as a mixture of two kinds or more, if miscible. If necessary to shorten the extraction time, it is optional to conduct the extraction treatment at an elevated temperature.
- alcoholic solvents such as methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, n-butyl alcohol, isobutyl alcohol, sec-butyl alcohol and tert-butyl alcohol, acetone, chloroform and n-hexane. These solvents can be used either singly or as a mixture of two kinds or more, if miscible
- the amount of water is in the range from 5 to 50 times by weight of the particulate pods.
- the thus formed extraction mixture is kept under agitation at a temperature of 80 to 100 °C or, preferably, at about 100 °C for 1 to 60 minutes or, in most cases, for 5 to 20 minutes followed by filtration of the mixture to give a clear extract solution by removing the solid remnant.
- the extraction treatment is undertaken at room temperature, the extraction mixture is agitated for 3 to 24 hours followed by filtra-
- the extract solution obtained in the above described manner is then subjected to a concentrating treatment by evaporating at least a part of water as the solvent at a temperature of 60 to 100 °C or, preferably, at about 70 °C or under a reduced pressure to give a syrupy liquid which exhibits strong active oxygen annihilating activity.
- This syrupy liquid can be further subjected to a f reeze-drymg treatment or spray-drying treatment into a powdery form by completely removing the solvent.
- an alcoholic solvent has an advantage as compared with water that extraction of the saccharide materials from the locust bean pods can be decreased relative to the SOD-like active substances so that the active oxygen annihilating agent contains the SOD-like substances in an increased concentration relative to the saccharides. It is of course possible to control relative extraction of the saccharides and the SOD-like active substances by modifying the polarity of the extractant solvent with addition of water to an alcoholic solvent depending on the active oxygen annihilating agent as desired.
- particulates of locust bean pods are added to 5 to 20 times by weight of an extractant solvent, which may be absolute alcohol or a mixture of water and ethyl alcohol containing 60% by weight or less or, preferably, 30% by weight or less of water, and the extraction mixture is agitated at room temperature for 15 minutes to 48 hours or, preferably for at least 24 hours followed by filtration to give a clear extract solution by removing the solid remnant.
- the extract solution is subjected to a concentrating treatment by evaporating ethyl alcohol under reduced pressure to give a syrupy liquid which can be freeze-dried or spray-dried into a powder.
- the inventive active oxygen annihilating agent is prepared in the form of a dry powder by completely removing the solvent in the concentrating treatment in consideration of the physiological effects of the solvent on the human body.
- the preparation form of the inventive active oxygen annihilating agent which can be a syrupy liquid or a powder, naturally depends on the particularly intended application.
- a syrupy preparation can be diluted as such with cold or hot water or with an alcoholic drink to give a health beverage.
- a powder preparation can be sprinkled as such over a food or can be used as an additive ingredient in food processing.
- a 50 g portion of particulates of dried locust bean pods was added to 250 g of hot water to give an extraction mixture which was boiled for 15 minutes and then filtered by using filter paper to give a filtrate.
- This aqueous solution was subjected to the test of active oxygen annihilating activity by the method of electron spin resonance (ESR) spectrometry to find an activity of 1.7 x 10 3 U/g.
- ESR electron spin resonance
- the aqueous solution was concentrated by heating to evaporate water into the form of a thick syrup of pasty consistency having a saccharide content of about 80%, which was again subjected to the ESR measurement to obtain a very high activity value of 1.0 x 10 4 U/g.
- This syrup had a good taste with sweetness and flavor resembling black raw sugar and was delicious for eating in a manner like a conventional jam spread over bread or in place of a maple syrup on pan-cakes.
- a tasty drink with a flavor like cocoa could be obtained by dissolving the above prepared syrup in an appropriate volume of hot water under agitation.
- Example 2 A tasty drink with a flavor like cocoa could be obtained by dissolving the above prepared syrup in an appropriate volume of hot water under agitation.
- the extract syrup of locust bean pods prepared in Example 1 was diluted with hot water to make a diluted syrup having a final saccharide content of 50% by weight, from which a pasty sweet having a jam-like consistency was prepared in a formulation including 45% by weight of the syrup calculated as the saccharide content, 1% by weight of xanthan gum and 0.1% by weight of docosahexaenoic acid (DHA) , the balance being water.
- DHA docosahexaenoic acid
- This pasty sweet had a consistency suitable for spreading over bread and had a masking effect on the offensive taste inherent in DHA. Accordingly, the pasty sweet could be evaluated as a valuable health food having active oxygen annihilating activity derived from locust bean pods without distastef ulness due to DHA.
- Example 3 Example 3
- the locust bean pods extract in the form of a syrup of about 80% saccharide concentration prepared in the same manner as in Example 1 was diluted with hot water to give a diluted extract solution of 10% saccharide concentration.
- a 1000 g portion of the diluted extract solution was taken in a dish tray and frozen at -85 °C and subjected to freeze- drying for 24 hours in a vacuum freeze-dry apparatus to find that the syrup was completely dried into the form of a cake which could easily be disintegrated into a light and fluffy powder of very fine particles.
- a 100 g portion of an unsweetened yogurt was admixed with 3 g of the thus freeze-dried powder to find that the powder could readily be dissolved in the yogurt and had moderate sweetness.
- the extract from locust bean pods serves not only as an active oxygen annihilating agent but also as a sweetening agent.
- An active oxygen annihilating agent suitable as an additive in health food products can be produced from locust bean pods which hitherto had almost no way of utilization but to be discarded as an agricultural waste material.
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Abstract
L'invention concerne un nouvel agent annihilant l'oxygène actif se présentant sous la forme d'un sirop ou d'une poudre adapté en tant qu'additif à des aliments diététiques pour réduire les influences nocives de l'oxygène actif sur le corps humain. L'agent annihilant l'oxygène actif est préparé à partir de gousses de caroube ayant présenté jusqu'à maintenant peu d'utilisabilité et rejetées comme matière résiduelle agricole. Ainsi, les particules broyées de gousses de caroube sont soumises à un traitement d'extraction à l'aide d'eau et d'alcool éthylique et la solution extraite est concentrée par évaporation d'au moins une partie du solvant pour obtenir un sirop pouvant être lyophilisé ou séché par pulvérisation en une poudre. Le sirop peut être transformé, par exemple, en une confiture savoureuse que l'on peut tartiner sur du pain.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11/63940 | 1999-03-10 | ||
JP11063940A JP2000253852A (ja) | 1999-03-10 | 1999-03-10 | 食品用活性酸素消去剤及びそれを含む飲食物 |
Publications (1)
Publication Number | Publication Date |
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WO2000053034A1 true WO2000053034A1 (fr) | 2000-09-14 |
Family
ID=13243860
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/JP1999/001397 WO2000053034A1 (fr) | 1999-03-10 | 1999-03-19 | Agent annihilant l'oxygene actif |
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JP (1) | JP2000253852A (fr) |
WO (1) | WO2000053034A1 (fr) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004057979A2 (fr) * | 2002-12-24 | 2004-07-15 | Nutrinova Nutrition Specialties & Food Ingedients Gmbh | Aliment dietetique pour influencer positivement la sante cardio-vasculaire |
FR2905857A1 (fr) * | 2006-09-20 | 2008-03-21 | Chanel Parfums Beaute Soc Par | Utilisation d'un extrait de pulpe de caroube pour hydrate la peau |
WO2008034821A1 (fr) | 2006-09-20 | 2008-03-27 | Chanel Parfums Beaute | Utilisation cosmétique d'agents actifs qui stimulent l'expression de la matriptase |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS4843628B1 (fr) * | 1970-08-03 | 1973-12-19 | ||
US4427707A (en) * | 1981-04-11 | 1984-01-24 | Henkel Kgaa | Method for the production of food additives with improved taste |
US5776756A (en) * | 1995-08-31 | 1998-07-07 | Toyo Hakko Co., Ltd. | Fermentation compositions having superoxide dismutating activity and an antihypertensive agent for treatment of constipation each having the superoxide dismutating activity |
-
1999
- 1999-03-10 JP JP11063940A patent/JP2000253852A/ja active Pending
- 1999-03-19 WO PCT/JP1999/001397 patent/WO2000053034A1/fr active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS4843628B1 (fr) * | 1970-08-03 | 1973-12-19 | ||
US4427707A (en) * | 1981-04-11 | 1984-01-24 | Henkel Kgaa | Method for the production of food additives with improved taste |
US5776756A (en) * | 1995-08-31 | 1998-07-07 | Toyo Hakko Co., Ltd. | Fermentation compositions having superoxide dismutating activity and an antihypertensive agent for treatment of constipation each having the superoxide dismutating activity |
Non-Patent Citations (2)
Title |
---|
DATABASE EPODOC European Patent Office; 29 September 1993 (1993-09-29), DONGHUA INDUSTRY CORP SHENYANG, XP002123526 * |
DATABASE WPI Section Ch Derwent World Patents Index; Class D13, AN 1973-81324U, XP002123527 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004057979A2 (fr) * | 2002-12-24 | 2004-07-15 | Nutrinova Nutrition Specialties & Food Ingedients Gmbh | Aliment dietetique pour influencer positivement la sante cardio-vasculaire |
WO2004057979A3 (fr) * | 2002-12-24 | 2005-09-01 | Nutrinova Gmbh | Aliment dietetique pour influencer positivement la sante cardio-vasculaire |
FR2905857A1 (fr) * | 2006-09-20 | 2008-03-21 | Chanel Parfums Beaute Soc Par | Utilisation d'un extrait de pulpe de caroube pour hydrate la peau |
WO2008034821A1 (fr) | 2006-09-20 | 2008-03-27 | Chanel Parfums Beaute | Utilisation cosmétique d'agents actifs qui stimulent l'expression de la matriptase |
Also Published As
Publication number | Publication date |
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JP2000253852A (ja) | 2000-09-19 |
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