WO2000026355A3 - Konstruktion von produktionsstämmen für die herstellung von substituierten phenolen durch gezielte inaktivierungen von genen des eugenol- und ferulasäure-katabolismus - Google Patents

Konstruktion von produktionsstämmen für die herstellung von substituierten phenolen durch gezielte inaktivierungen von genen des eugenol- und ferulasäure-katabolismus Download PDF

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Publication number
WO2000026355A3
WO2000026355A3 PCT/EP1999/007952 EP9907952W WO0026355A3 WO 2000026355 A3 WO2000026355 A3 WO 2000026355A3 EP 9907952 W EP9907952 W EP 9907952W WO 0026355 A3 WO0026355 A3 WO 0026355A3
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WO
WIPO (PCT)
Prior art keywords
eugenol
ferulic acid
construction
substituted phenols
acid catabolism
Prior art date
Application number
PCT/EP1999/007952
Other languages
English (en)
French (fr)
Other versions
WO2000026355A2 (de
Inventor
Juergen Rabenhorst
Alexander Steinbuechel
Horst Priefert
Joerg Overhage
Original Assignee
Haarmann & Reimer Gmbh
Juergen Rabenhorst
Alexander Steinbuechel
Horst Priefert
Joerg Overhage
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU10413/00A priority Critical patent/AU761093B2/en
Priority to JP2000579727A priority patent/JP2003533166A/ja
Priority to EP99953892A priority patent/EP1124947A2/de
Priority to BR9914930-3A priority patent/BR9914930A/pt
Priority to SK574-2001A priority patent/SK5742001A3/sk
Priority to CA002348962A priority patent/CA2348962A1/en
Application filed by Haarmann & Reimer Gmbh, Juergen Rabenhorst, Alexander Steinbuechel, Horst Priefert, Joerg Overhage filed Critical Haarmann & Reimer Gmbh
Priority to KR1020017005493A priority patent/KR20020022045A/ko
Priority to IL14227299A priority patent/IL142272A0/xx
Priority to HU0104772A priority patent/HUP0104772A3/hu
Publication of WO2000026355A2 publication Critical patent/WO2000026355A2/de
Publication of WO2000026355A3 publication Critical patent/WO2000026355A3/de
Priority to HK02103793.1A priority patent/HK1041902A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01016—Acetyl-CoA C-acyltransferase (2.3.1.16)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Die vorliegende Erfindung betrifft einen transformierten und/oder mutagenisierten ein- oder mehrzelligen Organismus, der dadurch gekennzeichnet ist, daß Enzyme des Eugenol- und/oder Ferulasäure-Katabolismus derart inaktiviert sind, daß eine Akkumulation der Intermediate Coniferylalkohol, Coniferylaldehyd, Ferulasäure, Vanillin und/oder Vanillinsäure erfolgt.
PCT/EP1999/007952 1998-10-31 1999-10-20 Konstruktion von produktionsstämmen für die herstellung von substituierten phenolen durch gezielte inaktivierungen von genen des eugenol- und ferulasäure-katabolismus WO2000026355A2 (de)

Priority Applications (10)

Application Number Priority Date Filing Date Title
JP2000579727A JP2003533166A (ja) 1998-10-31 1999-10-20 オイゲノールおよびフェルラ酸の異化作用の特異的不活性化遺伝子による置換フェノール生産のための生産株の構築
EP99953892A EP1124947A2 (de) 1998-10-31 1999-10-20 Konstruktion von produktionsstämmen für die herstellung von substituierten phenolen durch gezielte inaktivierungen von genen des eugenol- und ferulasäure-katabolismus
BR9914930-3A BR9914930A (pt) 1998-10-31 1999-10-20 Construção de estirpes de produção para a preparação de fenóis substituìdos por inativação almejada de genes do catabolismo dos ácidos eugenólico e ferúlico
SK574-2001A SK5742001A3 (en) 1998-10-31 1999-10-20 Construction of production strains for producing substituted phenols by specifically inactivating genes of the eugenol and ferulic acid catabolism
CA002348962A CA2348962A1 (en) 1998-10-31 1999-10-20 Construction of production strains for producing substituted phenols by specifically inactivating genes of the eugenol and ferulic acid catabolism
AU10413/00A AU761093B2 (en) 1998-10-31 1999-10-20 Construction of production strains for producing substituted phenols by specifically inactivating genes of the eugenol and ferulic acid catabolism
KR1020017005493A KR20020022045A (ko) 1998-10-31 1999-10-20 유게놀 및 페룰라산 분해대사의 유전자를 특이적으로불활성화함으로써 치환된 페놀을 생성하기 위한 생산균주의 구축
IL14227299A IL142272A0 (en) 1998-10-31 1999-10-20 Construction of production strains for producing substituted phenols by specifically inactivating genes of the eugenol and ferulic acid catabolism
HU0104772A HUP0104772A3 (en) 1998-10-31 1999-10-20 Construction of production strains for producing substituted phenols by specifically inactivating genes of the eugenol and ferulic acid catabolism
HK02103793.1A HK1041902A1 (zh) 1998-10-31 2002-05-21 經過特異性滅活丁子香酚和阿魏酸分解代謝的基因構建製備取代酚的生產菌株

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19850242A DE19850242A1 (de) 1998-10-31 1998-10-31 Konstruktion von Produktionsstämmen für die Herstellung von substituierten Phenolen durch gezielte Inaktivierung von Genen des Eugenol- und Ferulasäure-Katabolismus
DE19850242.7 1998-10-31

Publications (2)

Publication Number Publication Date
WO2000026355A2 WO2000026355A2 (de) 2000-05-11
WO2000026355A3 true WO2000026355A3 (de) 2000-11-09

Family

ID=7886266

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/007952 WO2000026355A2 (de) 1998-10-31 1999-10-20 Konstruktion von produktionsstämmen für die herstellung von substituierten phenolen durch gezielte inaktivierungen von genen des eugenol- und ferulasäure-katabolismus

Country Status (14)

Country Link
EP (1) EP1124947A2 (de)
JP (1) JP2003533166A (de)
KR (1) KR20020022045A (de)
CN (1) CN1325444A (de)
AU (1) AU761093B2 (de)
BR (1) BR9914930A (de)
CA (1) CA2348962A1 (de)
DE (1) DE19850242A1 (de)
HK (1) HK1041902A1 (de)
HU (1) HUP0104772A3 (de)
IL (1) IL142272A0 (de)
PL (1) PL348647A1 (de)
SK (1) SK5742001A3 (de)
WO (1) WO2000026355A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100830691B1 (ko) * 2006-11-21 2008-05-20 광주과학기술원 이소유제놀과 유제놀로부터 천연바닐린과 바닐린 산으로 생전환하는 신규 미생물
EP2721148B1 (de) 2011-06-17 2018-09-12 Symrise AG Mikroorganismen und verfahren zur herstellung substituierter phenole
US10036044B2 (en) 2013-07-22 2018-07-31 Basf Se Genetic engineering of Pseudomonas putida KT2440 for rapid and high yield production of vanillin from ferulic acid
CN103805640B (zh) * 2014-01-26 2016-04-06 东华大学 一种利用细菌氧化松伯醛制备阿魏酸的方法
EP3000888B1 (de) * 2014-09-29 2018-12-05 Symrise AG Verfahren zur Umwandlung von Ferulasäure in Vanillin
FR3041655B1 (fr) * 2015-09-29 2017-11-24 Lesaffre & Cie Nouvelles souches bacteriennes pour la production de vanilline
CN111019995B (zh) 2019-12-31 2021-04-27 厦门欧米克生物科技有限公司 一种以丁香酚为底物发酵生成香兰素的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05227980A (ja) * 1992-02-21 1993-09-07 Takasago Internatl Corp 発酵法によるバニリンおよびその関連化合物の製造法
EP0583687A2 (de) * 1992-08-17 1994-02-23 Haarmann & Reimer Gmbh Verfahren zur Herstellung substituierter Methoxyphenole und dafür geeignete Mikroorganismen
WO1997035999A2 (en) * 1996-03-23 1997-10-02 Institute Of Food Research Production of vanillin
EP0845532A2 (de) * 1996-11-29 1998-06-03 Haarmann & Reimer Gmbh Syntheseenzyme für die Herstellung von Coniferylalkohol, Coniferylaldehyd, Ferulasäure, Vanillin und Vanillinsäure und deren Verwendung

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05227980A (ja) * 1992-02-21 1993-09-07 Takasago Internatl Corp 発酵法によるバニリンおよびその関連化合物の製造法
EP0583687A2 (de) * 1992-08-17 1994-02-23 Haarmann & Reimer Gmbh Verfahren zur Herstellung substituierter Methoxyphenole und dafür geeignete Mikroorganismen
WO1997035999A2 (en) * 1996-03-23 1997-10-02 Institute Of Food Research Production of vanillin
EP0845532A2 (de) * 1996-11-29 1998-06-03 Haarmann & Reimer Gmbh Syntheseenzyme für die Herstellung von Coniferylalkohol, Coniferylaldehyd, Ferulasäure, Vanillin und Vanillinsäure und deren Verwendung

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 199340, Derwent World Patents Index; Class B05, AN 1993-316614, XP002139586 *
GASSON M J ET AL.: "Metabolism of ferulic acid to vanillin", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 7, 13 February 1998 (1998-02-13), pages 4163 - 4170, XP002139585 *

Also Published As

Publication number Publication date
KR20020022045A (ko) 2002-03-23
SK5742001A3 (en) 2001-12-03
HUP0104772A3 (en) 2003-10-28
CN1325444A (zh) 2001-12-05
DE19850242A1 (de) 2000-05-04
HUP0104772A2 (hu) 2002-03-28
AU1041300A (en) 2000-05-22
HK1041902A1 (zh) 2002-07-26
WO2000026355A2 (de) 2000-05-11
IL142272A0 (en) 2002-03-10
EP1124947A2 (de) 2001-08-22
CA2348962A1 (en) 2000-05-11
BR9914930A (pt) 2001-07-10
PL348647A1 (en) 2002-06-03
JP2003533166A (ja) 2003-11-11
AU761093B2 (en) 2003-05-29

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