WO2000019196A3 - Verfahren und testbesteck zur beurteilung der oxidativen modifikation proteinhaltiger stoffe mittels messung ihrer antiradikalen aktivität - Google Patents

Verfahren und testbesteck zur beurteilung der oxidativen modifikation proteinhaltiger stoffe mittels messung ihrer antiradikalen aktivität Download PDF

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Publication number
WO2000019196A3
WO2000019196A3 PCT/DE1999/003234 DE9903234W WO0019196A3 WO 2000019196 A3 WO2000019196 A3 WO 2000019196A3 DE 9903234 W DE9903234 W DE 9903234W WO 0019196 A3 WO0019196 A3 WO 0019196A3
Authority
WO
WIPO (PCT)
Prior art keywords
measuring
test kit
substances containing
containing protein
oxidative stress
Prior art date
Application number
PCT/DE1999/003234
Other languages
English (en)
French (fr)
Other versions
WO2000019196A2 (de
Inventor
Igor Popov
Gudrun Lewin
Original Assignee
Igor Popov
Gudrun Lewin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Igor Popov, Gudrun Lewin filed Critical Igor Popov
Priority to EP99969789A priority Critical patent/EP1036325B1/de
Priority to DE59914830T priority patent/DE59914830D1/de
Priority to JP2000572651A priority patent/JP2002525629A/ja
Publication of WO2000019196A2 publication Critical patent/WO2000019196A2/de
Publication of WO2000019196A3 publication Critical patent/WO2000019196A3/de
Priority to US09/584,712 priority patent/US6607919B1/en
Priority to US10/289,346 priority patent/US7125723B2/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Die Erfindung betrifft ein Verfahren und ein Testbesteck zur quantitativen Beurteilung der oxidativen Modifikation proteinhaltiger Stoffe bzw. des oxidativen Stresses in lebenden Organismen mit Hilfe der Messung der antiradikalen Eigenschaften ihrer proteinhaltigen Komponenten, z.B. Körperflüssigkeiten bzw. Gewebe. Das Wesen der Erfindung besteht darin, daß die Proben nach einer Abtrennung von niedermolekularen Antioxidantien, z.B. mittels der Gelchromatographie, in einem freiradikalgenerierenden System untersucht werden und die Meßergebnisse mit denen einer Probe gleicher Zusammensetzung von Aminosäuren, jedoch ohne oxidative Streßeinwirkung (Normwert), verglichen werden. Das Verfahren der Messung der antiradikalen Aktivität von Proteinen basiert auf dem erfindungsgemäßen Merkmal, daß einige nicht oxidativ modifizierte Aminosäuren als Bestandteile der Proteine diese Eigenschaft nicht besitzen, sie aber während der oxidativen Belastung erwerben.
PCT/DE1999/003234 1998-10-01 1999-09-30 Verfahren und testbesteck zur beurteilung der oxidativen modifikation proteinhaltiger stoffe mittels messung ihrer antiradikalen aktivität WO2000019196A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP99969789A EP1036325B1 (de) 1998-10-01 1999-09-30 Verfahren und testbesteck zur beurteilung der oxidativen modifikation proteinhaltiger stoffe mittels messung ihrer antiradikalen aktivität
DE59914830T DE59914830D1 (de) 1998-10-01 1999-09-30 Verfahren und testbesteck zur beurteilung der oxid messung ihrer antiradikalen aktivität
JP2000572651A JP2002525629A (ja) 1998-10-01 1999-09-30 タンパク質含有物質の抗酸化活性を測定することによる前記物質の酸化修飾評価の方法及び試験キット
US09/584,712 US6607919B1 (en) 1998-10-01 2000-06-01 Method and assay kit for evaluation of the oxidative modification of protein-containing substances
US10/289,346 US7125723B2 (en) 1998-10-01 2002-11-07 Method and assay kit for evaluation of the oxidative modification of protein-containing substances

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19846148.8 1998-10-01
DE19846148A DE19846148C2 (de) 1998-10-01 1998-10-01 Verfahren zur Beurteilung der oxidativen Modifikation proteinhaltiger Stoffe mittels Messung ihrer antiradikalen Aktivität

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/584,712 Continuation US6607919B1 (en) 1998-10-01 2000-06-01 Method and assay kit for evaluation of the oxidative modification of protein-containing substances

Publications (2)

Publication Number Publication Date
WO2000019196A2 WO2000019196A2 (de) 2000-04-06
WO2000019196A3 true WO2000019196A3 (de) 2000-05-25

Family

ID=7883670

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/003234 WO2000019196A2 (de) 1998-10-01 1999-09-30 Verfahren und testbesteck zur beurteilung der oxidativen modifikation proteinhaltiger stoffe mittels messung ihrer antiradikalen aktivität

Country Status (6)

Country Link
US (2) US6607919B1 (de)
EP (1) EP1036325B1 (de)
JP (1) JP2002525629A (de)
AT (1) ATE404867T1 (de)
DE (2) DE19846148C2 (de)
WO (1) WO2000019196A2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19846148C2 (de) * 1998-10-01 2002-10-10 Igor Popov Verfahren zur Beurteilung der oxidativen Modifikation proteinhaltiger Stoffe mittels Messung ihrer antiradikalen Aktivität
US7563433B2 (en) 2007-01-11 2009-07-21 Immunomedics, Inc. Methods and compositions for F-18 labeling of proteins, peptides and other molecules
US7993626B2 (en) 2007-01-11 2011-08-09 Immunomedics, Inc. Methods and compositions for F-18 labeling of proteins, peptides and other molecules
US7597876B2 (en) * 2007-01-11 2009-10-06 Immunomedics, Inc. Methods and compositions for improved F-18 labeling of proteins, peptides and other molecules
DE10259885B4 (de) * 2002-12-20 2007-12-06 Igor Dr. Popov Verfahren zur quantitativen Beurteilung des Schädigungsgrades von reinen Nukleotiden oder reinen nukleotidhaltigen Verbindungen
US7999003B2 (en) * 2003-08-26 2011-08-16 Mannatech, Incorporated Antioxidant compositions and methods thereto
US20050214413A1 (en) * 2003-08-26 2005-09-29 Mannatech, Inc. Methods and compositions for modified release of nutritional supplements
US20080227134A9 (en) * 2005-09-16 2008-09-18 Unigene Laboratories Inc. Glyoxylate assays and their use of inden tifying natural amidated compounds
US8153100B2 (en) * 2007-01-11 2012-04-10 Immunomedics, Inc. Methods and compositions for F-18 labeling of proteins, peptides and other molecules
US8889100B2 (en) 2007-01-11 2014-11-18 Immunomedics, Inc. Methods and compositions for improved F-18 labeling of proteins, peptides and other molecules
US8398956B2 (en) 2007-01-11 2013-03-19 Immunomedics, Inc. In vivo copper-free click chemistry for delivery of therapeutic and/or diagnostic agents
US8709382B2 (en) 2007-01-11 2014-04-29 Immunomedics, Inc. Methods and compositions for improved F-18 labeling of proteins, peptides and other molecules
US8545809B2 (en) 2007-01-11 2013-10-01 Immunomedics, Inc. Methods and compositions for improved 18F labeling of proteins, peptides and other molecules
US8709709B2 (en) 2007-05-18 2014-04-29 Luoxis Diagnostics, Inc. Measurement and uses of oxidative status
WO2008144481A1 (en) 2007-05-18 2008-11-27 Institute For Molecular Medicine, Inc. Measurement and uses of oxidative status
IN2012DN03177A (de) 2009-12-04 2015-09-25 Immunomedics Inc
DE102009059026A1 (de) 2009-12-18 2011-06-22 Gudrun Dr. 10115 Lewin Auswertungsverfahren zur Beurteilung des oxidativen Stress in Menschen und Tieren: Zweidimensionales Diagramm des oxidativen Stress (ZOS)
MX2013004852A (es) 2011-02-28 2013-10-01 Luoxis Diagnostics Inc Metodo y aparato para medir el potencial de oxidacion-reduccion.
DE102012003480A1 (de) 2012-02-21 2013-08-22 Gudrun Lewin Temperierte Meßkammer für photo/luminometrische Untersuchungen
EA201491808A1 (ru) 2012-04-19 2015-03-31 Луоксис Дайэгностикс, Инк. Многослойный гель
KR20150079550A (ko) 2012-10-23 2015-07-08 로익스 다이어그노스틱스, 아이엔씨. 생물학적 시료의 산화-환원 전위의 측정 및 사용하는 방법 및 시스템

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019979A1 (en) * 1990-06-12 1991-12-26 British Technology Group Ltd Antioxidant assay

Family Cites Families (3)

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FR2602592B1 (fr) * 1986-08-06 1989-06-30 Alain Baret Utilisation du systeme enzymatique xanthine-oxydase en immuno-analyse, procedes de dosage correspondants et coffrets de reactifs necessaires pour la mise en oeuvre de ces procedes.
JP3498960B2 (ja) * 1993-09-03 2004-02-23 デイド・ベーリング・マルブルク・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング 蛍光酸素チャンネリングイムノアッセイ
DE19846148C2 (de) * 1998-10-01 2002-10-10 Igor Popov Verfahren zur Beurteilung der oxidativen Modifikation proteinhaltiger Stoffe mittels Messung ihrer antiradikalen Aktivität

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019979A1 (en) * 1990-06-12 1991-12-26 British Technology Group Ltd Antioxidant assay

Non-Patent Citations (3)

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Title
AHOTUPA, M. ET AL.: "Simple methods of quantifying oxidation products and antioxidant potential of low density lipoproteins.", CLINICAL BIOCHEMISTRY, vol. 29, no. 2, 1996, pages 139 - 144, XP000879280 *
PACIFICI, R.E. AND DAVIES, K.J.A.: "Protein degradation as an index of oxidative stress", METHODS IN ENZYMOLOGY, vol. 186, 1990, pages 485 - 502, XP000879263 *
TWIGG, J. ET AL.: "Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: protective significance of seminal plasma", MOLECULAR HUMAN REPRODUCTION, vol. 4, no. 5, May 1998 (1998-05-01), pages 439 - 445, XP000879291 *

Also Published As

Publication number Publication date
WO2000019196A2 (de) 2000-04-06
DE19846148A1 (de) 2000-04-20
US6607919B1 (en) 2003-08-19
EP1036325A2 (de) 2000-09-20
US20030064523A1 (en) 2003-04-03
ATE404867T1 (de) 2008-08-15
DE19846148C2 (de) 2002-10-10
US7125723B2 (en) 2006-10-24
EP1036325B1 (de) 2008-08-13
JP2002525629A (ja) 2002-08-13
DE59914830D1 (de) 2008-09-25

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