WO2000008208A3 - Transcription inverse, amplification, et leurs amorces - Google Patents

Transcription inverse, amplification, et leurs amorces Download PDF

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Publication number
WO2000008208A3
WO2000008208A3 PCT/GB1999/002579 GB9902579W WO0008208A3 WO 2000008208 A3 WO2000008208 A3 WO 2000008208A3 GB 9902579 W GB9902579 W GB 9902579W WO 0008208 A3 WO0008208 A3 WO 0008208A3
Authority
WO
WIPO (PCT)
Prior art keywords
heeled
reverse transcription
primers
amplification processes
primers therefor
Prior art date
Application number
PCT/GB1999/002579
Other languages
English (en)
Other versions
WO2000008208A2 (fr
Inventor
Thomas Charles Freeman
Peter John Richardson
Alistair K Dixon
Original Assignee
Medical Res Council
Thomas Charles Freeman
Peter John Richardson
Alistair K Dixon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council, Thomas Charles Freeman, Peter John Richardson, Alistair K Dixon filed Critical Medical Res Council
Priority to AU52943/99A priority Critical patent/AU5294399A/en
Publication of WO2000008208A2 publication Critical patent/WO2000008208A2/fr
Publication of WO2000008208A3 publication Critical patent/WO2000008208A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Abstract

L'invention concerne un procédé permettant -'établir le profil d'expression de cellules isolées. Le procédé utilise une première amorce à « talon » (heeled) pour la transcription inverse d'ARNm dans un échantillon afin de produire des espèces d'ADNc à premier brin, et puis une deuxième population d'amorces à « talon » pour générer des ADNc à second brin. La partie « non-talon » (non-heeled) des secondes amorces « à talon » peuvent s'hybrider aux premiers brins transcrits inverses d'espèces d'ADNc, à raison d'au moins un brin sur toutes les longueurs correspondantes. La présence de séquences aléatoires et de séquences présélectionnées dans les secondes amorces permet de créer, à partir d'ARNm cellulaires, un profil d'ADNc qualitativement plus uniforme et donc plus représentatif. --
PCT/GB1999/002579 1998-08-05 1999-08-05 Transcription inverse, amplification, et leurs amorces WO2000008208A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU52943/99A AU5294399A (en) 1998-08-05 1999-08-05 Reverse transcription and amplification processes and primers therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9817055.8 1998-08-05
GBGB9817055.8A GB9817055D0 (en) 1998-08-05 1998-08-05 Reverse transcription and amplification processes and primers therefore

Publications (2)

Publication Number Publication Date
WO2000008208A2 WO2000008208A2 (fr) 2000-02-17
WO2000008208A3 true WO2000008208A3 (fr) 2000-05-11

Family

ID=10836764

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/002579 WO2000008208A2 (fr) 1998-08-05 1999-08-05 Transcription inverse, amplification, et leurs amorces

Country Status (3)

Country Link
AU (1) AU5294399A (fr)
GB (1) GB9817055D0 (fr)
WO (1) WO2000008208A2 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7771946B2 (en) 2001-03-09 2010-08-10 Nugen Technologies, Inc. Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences
US7771934B2 (en) 2000-12-13 2010-08-10 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US8143001B2 (en) 2003-12-29 2012-03-27 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US8465950B2 (en) 2003-04-14 2013-06-18 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001006004A2 (fr) * 1999-07-19 2001-01-25 Cambridge University Technical Services Ltd. Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2002048352A1 (fr) * 2000-12-12 2002-06-20 Aisin Seiki Kabushiki Kaisha Methode d'analyse d'expression genique
EP1275738A1 (fr) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Procédé pour la synthèse aléatoire et l'amplification d'ADNc
AU2002341752B2 (en) 2001-09-19 2008-04-03 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
US7414111B2 (en) 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
JP2005519621A (ja) 2002-03-11 2005-07-07 ニューゲン テクノロジーズ， インコーポレイテッド ３’一本鎖部分を含む二本鎖ｄｎａを生成する方法および組換えのためのこれらの複合体の使用
GB0216026D0 (en) * 2002-07-10 2002-08-21 Cambridge Biotechnology Ltd Nucleic acid amplification method
GB2513793B (en) 2012-01-26 2016-11-02 Nugen Tech Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP2861787B1 (fr) 2012-06-18 2017-09-20 Nugen Technologies, Inc. Compositions et procédés pour la sélection négative de séquences d'acide nucléique indésirable
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
WO2015131107A1 (fr) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Séquençage au bisulfite de représentation réduite avec adaptateurs de diversité
JP6803327B2 (ja) 2014-08-06 2020-12-23 ニューゲン テクノロジーズ， インコーポレイテッド 標的化されたシークエンシングからのデジタル測定値
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991015601A1 (fr) * 1990-04-05 1991-10-17 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Reaction en chaine de polymerase specifique de reproduction d'a.r.n. modifiee
WO1998008973A1 (fr) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Amplification globale d'acides nucleiques

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991015601A1 (fr) * 1990-04-05 1991-10-17 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Reaction en chaine de polymerase specifique de reproduction d'a.r.n. modifiee
WO1998008973A1 (fr) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Amplification globale d'acides nucleiques

Non-Patent Citations (6)

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Title
CHEUNG AND NELSON: "WHOLE GENOME AMPLIFICATION USING A DEGENERATE OLIGONUCLEOTIDE PRIMER ALLOWS HUNDREDS OF GENOTYPES TO BE PERFORMED ON LESS THAN ONE NANOGRAM OF GENOMIC DNA", PNAS, vol. 93, 1996, pages 14676 - 14679, XP002128316 *
DIXON ET AL.: "EXPRESSION PROFILING OF SINGLE CELLS USING 3 PRIME END AMPLIFICATION (TPEA) PCR", NUCLEIC ACIDS RESEARCH, vol. 26, no. 19, October 1998 (1998-10-01), pages 4426 - 4431, XP002128317 *
FROUSSARD: "A RANDOM-PCR METHOD (rPCR) TO CONSTRUCT WHOLE cDNA LIBRARY FROM LOW AMOUNTS OF RNA", NUCLEIC ACIDS RESEARCH, vol. 20, no. 11, 1992, pages 2900, XP002128315 *
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS,US,ACADEMIC PRESS, SAN DIEGO, vol. 13, 1992, pages 718-725, XP000199116, ISSN: 0888-7543 *
ZHAO ET AL.: "3'-END cDNA POOL SUITABLE FOR DIFFERENTIAL DISPLAY FROM A SMALL NUMBER OF CELLS", BIOTECHNIQUES, vol. 24, May 1998 (1998-05-01), pages 842 - 852, XP002128314 *
ZHAO ET AL.: "NEW PRIMER STRATEGY IMPROVES PRECISION OF DIFFERENTIAL DISPLAY", BIOTECHNIQUES, vol. 18, no. 5, 1995, pages 842 - 850, XP002128267 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7771934B2 (en) 2000-12-13 2010-08-10 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US8334116B2 (en) 2000-12-13 2012-12-18 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US8071311B2 (en) 2001-03-09 2011-12-06 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
US9181582B2 (en) 2001-03-09 2015-11-10 Nugen Technologies, Inc. Compositions for amplification of RNA sequences using composite primers
US7771946B2 (en) 2001-03-09 2010-08-10 Nugen Technologies, Inc. Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences
US8465950B2 (en) 2003-04-14 2013-06-18 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US9175325B2 (en) 2003-04-14 2015-11-03 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US8143001B2 (en) 2003-12-29 2012-03-27 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8852867B2 (en) 2005-09-07 2014-10-07 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing

Also Published As

Publication number Publication date
AU5294399A (en) 2000-02-28
WO2000008208A2 (fr) 2000-02-17
GB9817055D0 (en) 1998-09-30

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