WO1999047649A3 - Polymerasenchimären - Google Patents

Polymerasenchimären Download PDF

Info

Publication number
WO1999047649A3
WO1999047649A3 PCT/EP1999/001674 EP9901674W WO9947649A3 WO 1999047649 A3 WO1999047649 A3 WO 1999047649A3 EP 9901674 W EP9901674 W EP 9901674W WO 9947649 A3 WO9947649 A3 WO 9947649A3
Authority
WO
WIPO (PCT)
Prior art keywords
chimeras
polymerase
relates
domains
polymerase chimeras
Prior art date
Application number
PCT/EP1999/001674
Other languages
English (en)
French (fr)
Other versions
WO1999047649A2 (de
WO1999047649A9 (de
Inventor
Bruno Frey
Britta Villbrandt
Dietmar Schomburg
Harald Sobek
Waltraud Ankenbauer
Original Assignee
Roche Diagnostics Gmbh
Bruno Frey
Britta Villbrandt
Dietmar Schomburg
Harald Sobek
Waltraud Ankenbauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Bruno Frey, Britta Villbrandt, Dietmar Schomburg, Harald Sobek, Waltraud Ankenbauer filed Critical Roche Diagnostics Gmbh
Priority to EP99915607A priority Critical patent/EP1112351B1/de
Priority to DE59914254T priority patent/DE59914254D1/de
Priority to US09/623,326 priority patent/US6607883B1/en
Priority to JP2000536832A priority patent/JP3553018B2/ja
Publication of WO1999047649A2 publication Critical patent/WO1999047649A2/de
Publication of WO1999047649A9 publication Critical patent/WO1999047649A9/de
Publication of WO1999047649A3 publication Critical patent/WO1999047649A3/de
Priority to US10/456,129 priority patent/US7244602B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Solid State Image Pick-Up Elements (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Gegenstand der vorliegenden Erfindung sind Polymerasenchimären, die aus Aminosäurefragmenten, die Domänen repräsentieren, bestehen und die - im Hinblick auf eine bestimmte Verwendung - vorteilhafte Eigenschaften von natürlich vorkommenden Polymerasen in sich vereinen. Überraschenderweise konnte gezeigt werden, daß die Domänen aus den unterschiedlichen Enzymen in der Chimäre aktiv sind und ein kooperatives Verhalten zeigen. Weiterhin ist Gegenstand der vorliegenden Erfindung ein Verfahren zur Herstellung der erfindungsgemäßen Chimären sowie die Verwendung dieser Chimären bei der Synthese von Nukleinsäuren z.B. während der Polymerase-Ketten-Reaktion. Ein weiterer Gegenstand der vorliegenden Erfindung ist ein Kit, der die erfindungsgemäßen Polymerasenchimären enthält.
PCT/EP1999/001674 1998-03-13 1999-03-15 Polymerasenchimären WO1999047649A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP99915607A EP1112351B1 (de) 1998-03-13 1999-03-15 Polymerasenchimären
DE59914254T DE59914254D1 (de) 1998-03-13 1999-03-15 Polymerasenchimären
US09/623,326 US6607883B1 (en) 1998-03-13 1999-03-15 Polymerase chimeras
JP2000536832A JP3553018B2 (ja) 1998-03-13 1999-03-15 ポリメラーゼキメラ
US10/456,129 US7244602B2 (en) 1998-03-13 2003-06-06 Polymerase chimeras

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19810879A DE19810879A1 (de) 1998-03-13 1998-03-13 Polymerasenchimären
DE19810879.6 1998-03-13

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09623326 A-371-Of-International 1999-03-15
US09/623,326 A-371-Of-International US6607883B1 (en) 1998-03-13 1999-03-15 Polymerase chimeras
US10/456,129 Division US7244602B2 (en) 1998-03-13 2003-06-06 Polymerase chimeras

Publications (3)

Publication Number Publication Date
WO1999047649A2 WO1999047649A2 (de) 1999-09-23
WO1999047649A9 WO1999047649A9 (de) 1999-11-18
WO1999047649A3 true WO1999047649A3 (de) 1999-12-23

Family

ID=7860740

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/001674 WO1999047649A2 (de) 1998-03-13 1999-03-15 Polymerasenchimären

Country Status (6)

Country Link
US (2) US6607883B1 (de)
EP (1) EP1112351B1 (de)
JP (2) JP3553018B2 (de)
AT (1) ATE356866T1 (de)
DE (2) DE19810879A1 (de)
WO (1) WO1999047649A2 (de)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9921318D0 (en) * 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
EP1088891B1 (de) 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation
DE60133992D1 (de) 2000-02-17 2008-06-26 Qiagen Gmbh Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
EP1301205A1 (de) * 2000-07-12 2003-04-16 Invitrogen Corporation Hochempfindliche polymerasen und ihre verwendungen
DE10049211A1 (de) 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
WO2003025132A2 (en) * 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
US20030228611A1 (en) * 2002-05-01 2003-12-11 President And Fellows Of Harvard College Nucleic acid memory device
US20060234227A1 (en) * 2002-05-14 2006-10-19 Alexei Slesarev Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes
US8283148B2 (en) * 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
GB2416538B (en) * 2004-07-26 2008-07-30 Bioline Ltd Chimeric DNA polymerase
EP1963501A4 (de) * 2005-12-22 2009-04-22 Pacific Biosciences California Proteinherstellungsstrategien zur optimierung der aktivität von proteinen mit oberflächenhaftung
ATE518010T1 (de) * 2005-12-22 2011-08-15 Pacific Biosciences California Aktive oberflächengekoppelte polymerasen
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US8889348B2 (en) 2006-06-07 2014-11-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by nanopore using modified nucleotides
AU2007309504B2 (en) * 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
JP5809059B2 (ja) 2008-11-03 2015-11-10 カパ バイオシステムズ， インコーポレイテッド 改変ａ型ｄｎａポリメラーゼ
WO2010062776A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
US8603792B2 (en) 2009-03-27 2013-12-10 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8845880B2 (en) 2010-12-22 2014-09-30 Genia Technologies, Inc. Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
JP2015525077A (ja) 2012-06-15 2015-09-03 ジェニア・テクノロジーズ・インコーポレイテッド チップの構成および高精度な核酸配列決定
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
EP3640349A3 (de) 2013-10-23 2020-07-29 Roche Sequencing Solutions, Inc. Schnelle molekulare erfassung mit nanoporen
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
JP6573899B2 (ja) 2013-11-17 2019-09-11 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ 分子をプローブし、検出し、及び分析するための、外部光源を備えた集積デバイス
WO2015085230A1 (en) * 2013-12-06 2015-06-11 Bio-Rad Laboratories, Inc. Fusion polymerases
JP6812341B2 (ja) 2014-08-08 2021-01-13 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ 分子の探索、検出及び解析のための光学システム及びアッセイチップ
CN106796176B (zh) 2014-08-08 2021-02-05 宽腾矽公司 用于对分子进行探测、检测和分析的带外部光源的集成装置
KR102384909B1 (ko) 2014-08-08 2022-04-11 퀀텀-에스아이 인코포레이티드 수신된 광자들의 시간 비닝을 위한 집적 디바이스
US9447445B2 (en) 2014-08-27 2016-09-20 New England Biolabs, Inc. Synthon formation
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
US10364420B2 (en) 2015-12-07 2019-07-30 Bio-Rad Laboratories, Inc Dimeric reverse transcriptase
AU2017219894B2 (en) 2016-02-17 2021-12-09 Tesseract Health, Inc. Sensor and device for lifetime imaging and detection applications
KR20230052995A (ko) 2016-12-19 2023-04-20 퀀텀-에스아이 인코포레이티드 시퀀싱 반응을 위한 중합 효소
BR112019012540A2 (pt) 2016-12-22 2019-11-12 Quantum-Si Incorporated fotodetector integrado com pixel de acondicionamento direto
CN111684064A (zh) 2018-01-19 2020-09-18 生物辐射实验室股份有限公司 突变dna聚合酶
WO2019246328A1 (en) 2018-06-22 2019-12-26 Quantum-Si Incorporated Integrated photodetector with charge storage bin of varied detection time
CN109825589B (zh) * 2019-03-29 2022-09-06 深圳市亚辉龙生物科技股份有限公司 Loc112268236基因的应用、检测甲基化的核酸组合物及其试剂盒和检测方法
JP2022539560A (ja) 2019-06-28 2022-09-12 クアンタム－エスアイ インコーポレイテッド 配列決定反応のための重合酵素
CN113755465A (zh) * 2021-09-23 2021-12-07 武汉爱博泰克生物科技有限公司 嵌合体dna聚合酶及其制备方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0482714A1 (de) * 1990-10-26 1992-04-29 Johnson & Johnson Clinical Diagnostics, Inc. Erhöhte Produktion von thermus aquaticus DNA Polymerase in E. coli
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
WO1997029209A1 (en) * 1996-02-09 1997-08-14 President And Fellows Of Harvard College Dna polymerase with modified processivity
EP0892058A2 (de) * 1997-07-09 1999-01-20 F. Hoffmann-La Roche Ag Mutierte chimäre DNS-Polymerasen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6077664A (en) 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
EP0482714A1 (de) * 1990-10-26 1992-04-29 Johnson & Johnson Clinical Diagnostics, Inc. Erhöhte Produktion von thermus aquaticus DNA Polymerase in E. coli
WO1997029209A1 (en) * 1996-02-09 1997-08-14 President And Fellows Of Harvard College Dna polymerase with modified processivity
EP0892058A2 (de) * 1997-07-09 1999-01-20 F. Hoffmann-La Roche Ag Mutierte chimäre DNS-Polymerasen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RUI SOUSA ET AL: "SINGLE CRYSTALS OF A CHIMERIC T7/T3 RNA POLYMERASE WITH T3 PROMOTER SPECIFICITY", JOURNAL OF CRYSTAL GROWTH, vol. 122, no. 1 / 04, 2 August 1992 (1992-08-02), pages 366 - 374, XP000306506 *

Also Published As

Publication number Publication date
US7244602B2 (en) 2007-07-17
DE19810879A1 (de) 1999-09-16
JP2002506637A (ja) 2002-03-05
ATE356866T1 (de) 2007-04-15
US6607883B1 (en) 2003-08-19
JP3553018B2 (ja) 2004-08-11
EP1112351B1 (de) 2007-03-14
JP2004180690A (ja) 2004-07-02
WO1999047649A2 (de) 1999-09-23
US20040058362A1 (en) 2004-03-25
EP1112351A2 (de) 2001-07-04
WO1999047649A9 (de) 1999-11-18
DE59914254D1 (de) 2007-04-26

Similar Documents

Publication Publication Date Title
WO1999047649A3 (de) Polymerasenchimären
EP1700915A3 (de) Enzymatische DNS-Moleküle
WO2003016475A3 (en) Nucleic acid and amino acid sequences involved in pain
WO2000008196A3 (en) Urate oxidase
WO2001079465A3 (en) Nucleic acids encoding polypeptides having haloperoxidase activity
WO2001051621A3 (en) Dna polymerase mutant having one or more mutations in the active site
WO2002010358A3 (en) Nucleotide incorporating enzymes
EP2275546A3 (de) Enzymaktive DNS-moleküle
WO2001079462A3 (en) Nucleic acids encoding polypeptides having haloperoxidase activity
WO2002077165A3 (en) Glycoconjugate synthesis using a pathway-engineered organism
WO2001047944A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
CA2295999A1 (en) Nik proteins, nucleic acids and methods
WO2002102825A3 (en) Fluorescently labelled locked nucleic acids
CA2281895A1 (en) Ikb kinases
WO2000029623A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
WO1999051727A3 (de) Menschliche nukleinsäuresequenzen aus ovarnormalgewebe
CA2294476A1 (en) Semaphorin receptors
WO1997027276A3 (en) Nucleic acids encoding polypeptides having absidia lipase activity
WO2002016617A3 (en) Myrosinase from brevicoryne brassicae
WO1999065938A3 (en) Fy7 polymerase
WO1999055915A3 (en) IDENTIFICATION OF POLYMORPHISMS IN THE PCTG4 REGION OF Xq13
WO1999047655A3 (de) Menschliche nukleinsaeurefragmente, deren expression in brustnormalgewebe erhöht ist
WO2000000593A3 (de) Sphingolipid-desaturase
WO2001048245A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
WO2001040521A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: C2

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

COP Corrected version of pamphlet

Free format text: PAGES 13/31-14/31 AND 16/31-20/31, DRAWINGS, REPLACED BY NEW PAGES 13/31-14/31 AND 16/31-20/31

AK Designated states

Kind code of ref document: A3

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

WWE Wipo information: entry into national phase

Ref document number: 1999915607

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 09623326

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 1999915607

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 1999915607

Country of ref document: EP