WO1999022023A3 - Procede de caracterisation de microorganismes - Google Patents

Procede de caracterisation de microorganismes Download PDF

Info

Publication number
WO1999022023A3
WO1999022023A3 PCT/EP1998/006863 EP9806863W WO9922023A3 WO 1999022023 A3 WO1999022023 A3 WO 1999022023A3 EP 9806863 W EP9806863 W EP 9806863W WO 9922023 A3 WO9922023 A3 WO 9922023A3
Authority
WO
WIPO (PCT)
Prior art keywords
organisms
micro
identifying micro
taxes
various
Prior art date
Application number
PCT/EP1998/006863
Other languages
German (de)
English (en)
Other versions
WO1999022023A2 (fr
Inventor
Matthias Leiser
Bernd Epping
Original Assignee
Mira Diagnostica Gmbh
Matthias Leiser
Bernd Epping
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mira Diagnostica Gmbh, Matthias Leiser, Bernd Epping filed Critical Mira Diagnostica Gmbh
Priority to AU13370/99A priority Critical patent/AU1337099A/en
Publication of WO1999022023A2 publication Critical patent/WO1999022023A2/fr
Publication of WO1999022023A3 publication Critical patent/WO1999022023A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé de caractérisation de microorganismes de différentes taxies, spécifiés au tableau 1, dans un échantillon pouvant contenir une pluralité de différents microorganismes de ces taxies, au moyen de techniques d'hybridation d'acides nucléiques, en utilisant des oligonucléotides de Séq. ID. No 1 à 62 comme sondes, tout en conservant un résultat d'hybridation, procédé dans lequel, pour chaque microorganismes à caractériser, au moins un résultat d'hybridation est maintenu.
PCT/EP1998/006863 1997-10-29 1998-10-29 Procede de caracterisation de microorganismes WO1999022023A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU13370/99A AU1337099A (en) 1997-10-29 1998-10-29 Method for identifying micro-organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19747731 1997-10-29
DE19747731.3 1997-10-29

Publications (2)

Publication Number Publication Date
WO1999022023A2 WO1999022023A2 (fr) 1999-05-06
WO1999022023A3 true WO1999022023A3 (fr) 1999-09-16

Family

ID=7846971

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/006863 WO1999022023A2 (fr) 1997-10-29 1998-10-29 Procede de caracterisation de microorganismes

Country Status (2)

Country Link
AU (1) AU1337099A (fr)
WO (1) WO1999022023A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19941359C2 (de) * 1999-08-31 2002-12-05 Gsf Forschungszentrum Umwelt Schneller, hochspezifischer Nachweis von Pseudomonas aeruginosa durch Mehrfachsondendetektion
DE19945964A1 (de) 1999-09-24 2001-04-05 Biotecon Diagnostics Gmbh Verfahren und Nukleinsäuren zum Nachweis von brauereirelevanten Mikroorganismen
EP1255867A2 (fr) 2000-02-18 2002-11-13 Science Applications International Corporation Methode permettant de detecter une entite biologique dans un echantillon
EP1307587A2 (fr) 2000-03-03 2003-05-07 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES SYSTEME D'HYBRIDATION MULTIPLEX DESTINE A L'IDENTIFICATION DE i MYCOBACTERIUM /i PATHOGENE ET PROCEDE D'UTILISATION
US7455966B1 (en) 2000-05-01 2008-11-25 Science Applications International Corporation System and method for detecting a biological entity in a water sample
JP2004504068A (ja) * 2000-07-27 2004-02-12 ザ　オーストラリアン　ナショナル　ユニバーシティー コンビナトリアル・プローブ及びそのための用途
DE10124342A1 (de) * 2001-05-18 2002-11-28 Biotecon Diagnostics Gmbh Verfahren zum Nachweis von Mikroorganismen der Spezies Yersinia pestis/Yersinia pseudotuberculosis und/oder zur Differzierung zwischen Yersinia pestis und Yersinia pseudotuberculosis
JP2004313181A (ja) * 2003-04-02 2004-11-11 Canon Inc 感染症起炎菌検出用プローブ及びプローブセット、ならびに担体及び遺伝子検査方法
WO2008130394A2 (fr) 2006-11-30 2008-10-30 The Regents Of The University Of California Réseau pour détecter des microbes
CA2766312C (fr) * 2009-06-26 2020-04-14 Gary L. Andersen Procedes et systemes d'analyse phylogenetique
US9725770B2 (en) 2012-03-06 2017-08-08 The Regents Of The University Of California Methods and compositions for identification of source of microbial contamination in a sample

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0318245A2 (fr) * 1987-11-24 1989-05-31 Gen-Probe Incorporated Moyen et méthode pour augmenter l'hybridation d'acide nucléique
JPH05100A (ja) * 1990-11-09 1993-01-08 Nippon Flour Mills Co Ltd 細菌の薬剤感受性試験法
WO1993004199A2 (fr) * 1991-08-20 1993-03-04 Scientific Generics Limited Procedes de detection et de quantification d'acides nucleiques, et de production d'acides nucleiques marques, immobilises
WO1995034574A1 (fr) * 1994-06-16 1995-12-21 Microprobe Corporation Detection rapide et de haute precision de mycobacteries resistant aux antibiotiques a l'aide de sondes oligonucleotidiques specifiques contre les precurseurs d'adn ribosomique
WO1996000298A1 (fr) * 1994-06-24 1996-01-04 Innogenetics N.V. Detection, identification et differentiation simultanees de taxa d'eubacteriales a l'aide d'une technique d'hybridation
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
WO1997041253A1 (fr) * 1996-04-26 1997-11-06 Mira Diagnostica Gmbh Procede de depistage de micro-organismes dans des melanges par amplification modulaire par reaction en chaine de la polymerase

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
EP0318245A2 (fr) * 1987-11-24 1989-05-31 Gen-Probe Incorporated Moyen et méthode pour augmenter l'hybridation d'acide nucléique
JPH05100A (ja) * 1990-11-09 1993-01-08 Nippon Flour Mills Co Ltd 細菌の薬剤感受性試験法
WO1993004199A2 (fr) * 1991-08-20 1993-03-04 Scientific Generics Limited Procedes de detection et de quantification d'acides nucleiques, et de production d'acides nucleiques marques, immobilises
WO1995034574A1 (fr) * 1994-06-16 1995-12-21 Microprobe Corporation Detection rapide et de haute precision de mycobacteries resistant aux antibiotiques a l'aide de sondes oligonucleotidiques specifiques contre les precurseurs d'adn ribosomique
WO1996000298A1 (fr) * 1994-06-24 1996-01-04 Innogenetics N.V. Detection, identification et differentiation simultanees de taxa d'eubacteriales a l'aide d'une technique d'hybridation
WO1997041253A1 (fr) * 1996-04-26 1997-11-06 Mira Diagnostica Gmbh Procede de depistage de micro-organismes dans des melanges par amplification modulaire par reaction en chaine de la polymerase

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BRAUN-HOWLAND E B ET AL: "USE OF A SIMPLIFIED CELL BLOT TECHNIQUE AND 16S RRNA-DIRECTED PROBES FOR IDENTIFICATION OF COMMON ENVIRONMENTAL ISOLATES", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 59, no. 10, October 1993 (1993-10-01), pages 3219 - 3224, XP000604178 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002108049, Database accession no. E04712 *
EHRMANN M ET AL: "REVERSE DOT BLOT HYBRIDIZATION: A USEFUL METHOD FOR THE DIRECT IDENTIFICATION OF LACTIC ACID BACTERIA IN FERMENTED FOOD", FEMS MICROBIOLOGY LETTERS, 1994, pages 143 - 149, XP000612834 *
ENRIGHT M C ET AL: "PHYLOGENETIC RELATIONSHIPS BETWEEN SOME MEMBERS OF THE GENERA NEISSERIA, ACINETOBACTER, MORAXELLA, AND KINGELLA BASED ON PARTIAL 16S RIBOSOMAL DNA SEQUENCE ANALYSIS", INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, vol. 44, no. 3, July 1994 (1994-07-01), pages 387 - 391, XP000605280 *
GUSCHIN D Y ET AL: "OLIGONUCLEOTIDE MICROCHIPS AS GENOSENSORS FOR DETERMINATIVE AND ENVIRONMENTAL STUDIES IN MICROBIOLOGY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 6, June 1997 (1997-06-01), pages 2397 - 2402, XP002064989 *
IBRAHIM A ET AL: "Phylogenetic relationship of the twenty-one DNA groups of the genus Acinetobacter as revealed by 16S ribosomal DNA sequence analysis.", INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, (1997 JUL) 47 (3) 837-41., XP002096199 *
RAINEY F A ET AL: "The phylogenetic structure of the genus Acinetobacter", FEMS MICROBIOLOGY LETTERS, (1994 DEC 15) 124 (3) 349-53., XP002096200 *
WAGNER M ET AL: "Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, (1994 MAR) 60 (3) 792-800., XP002096198 *

Also Published As

Publication number Publication date
WO1999022023A2 (fr) 1999-05-06
AU1337099A (en) 1999-05-17

Similar Documents

Publication Publication Date Title
DE60034878T2 (de) Verfahren zur Amplifizierung einer Nukleinsäuresequenz unter Verwendung eines chimären Primers
PL322611A1 (en) Methods of obtaining l-amini acid by fermentation, dna as well as micro-organism
CA2320666A1 (fr) Nucleases 5' derivees d'adn polymerase thermostable
CA2219891A1 (fr) Procedes et reactifs pour combiner une amplification par reaction en chaine de la polymerase et une technique de sondage par hybridation
WO2001092572A1 (fr) Ensemble et procede de determination du type de hla
CA2172248A1 (fr) Detection et quantification d'une sequence d'acide nucleique par marquage electrochimioluminescent
ATE386139T1 (de) Verfahren, basierend auf einer polynukleotidmatrix, zur identifizierung von mikroorganismen
FR2685346B1 (fr) Procede de preparation d'arn double-brin, et ses applications.
EP0800587A4 (fr) Hybridation comparative a fluorescence a des ensembles d'acides nucleiques
EP0822256A3 (fr) Polymérase d'ADN thermostable modifiée et composition ayant une polymérase d'ADN pour l'amplification d'acides nucléiques
DE69609345D1 (de) Verfahren zur herstellen einer trägerplatte für eine zweidimensionales netwerk von mikrohohlen ins besonderes für biologische tests oder kulturen
CA2310854A1 (fr) Isolement et identification de nouvelles polymerases
GB9210176D0 (en) Chemical method
WO1999022023A3 (fr) Procede de caracterisation de microorganismes
DE59310281D1 (de) Verfahren zur präparierung und hybridisierung von spezifischen proben
RU98105786A (ru) Модифицированные праймеры
CA2230967A1 (fr) Dosages ameliores des acides nucleiques
WO1993016094A3 (fr) Utilisation de n-nucleosides fluorescents et de leurs analogues structurels fluorescents
PL335860A1 (en) Method of in vitro constructing a dna library
DE69941441D1 (de) Methode für DNA synthese
EP0705905A3 (fr) Procédé pour la détection sensible d'acides nucléiques
MXPA03001879A (es) Metodo y sistema para detectar acidos nucleicos.
AU587188B2 (en) Nucleic acid hybridazation assay employing detectable anti- hybrid antibodies
NZ538852A (en) Selection and isolation of living cells using mRNA-binding probes
CA2282704A1 (fr) Dosage de nucleotides dans une solution a l'aide de sondes d'acides nucleiques peptidiques

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AU BA BB BG BR BY CA CN CU CZ DE EE GD GE HR HU ID IL IS JP KP KR LC LK LR LT LV MG MK MN MX NO NZ PL RO RU SG SI SK SL TR TT UA US UZ VN YU

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AU BA BB BG BR BY CA CN CU CZ DE EE GD GE HR HU ID IL IS JP KP KR LC LK LR LT LV MG MK MN MX NO NZ PL RO RU SG SI SK SL TR TT UA US UZ VN YU

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: KR

NENP Non-entry into the national phase

Ref country code: CA

122 Ep: pct application non-entry in european phase