WO1998006267A1 - Procede et immobilisat pour lutter contre les phytoparasites - Google Patents

Procede et immobilisat pour lutter contre les phytoparasites Download PDF

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Publication number
WO1998006267A1
WO1998006267A1 PCT/EP1997/004080 EP9704080W WO9806267A1 WO 1998006267 A1 WO1998006267 A1 WO 1998006267A1 EP 9704080 W EP9704080 W EP 9704080W WO 9806267 A1 WO9806267 A1 WO 9806267A1
Authority
WO
WIPO (PCT)
Prior art keywords
microorganisms
outer shell
seed
nutrient medium
immobilisat
Prior art date
Application number
PCT/EP1997/004080
Other languages
German (de)
English (en)
Other versions
WO1998006267A9 (fr
Inventor
Jürgen Engelhardt
Wolfgang Koch
Jörn-Bernd PANNEK
Klaus-Dieter Vorlop
Arant Vallabhbha Patel
Original Assignee
Wolff Walsrode Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wolff Walsrode Ag filed Critical Wolff Walsrode Ag
Priority to AU43779/97A priority Critical patent/AU4377997A/en
Priority to JP50933398A priority patent/JP2001504445A/ja
Priority to EP97941898A priority patent/EP0934001A1/fr
Publication of WO1998006267A1 publication Critical patent/WO1998006267A1/fr
Publication of WO1998006267A9 publication Critical patent/WO1998006267A9/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27—Pseudomonas

Definitions

  • the invention relates to a method for controlling plant pests by introducing microorganisms which destroy the plant pests into a networked support structure in contact with a plant or plant seeds.
  • the invention further relates to an immobilizate for biological pest control
  • Enzymes have been known for a long time.
  • a wide variety of polyelectrolyte systems have been proposed for immobilization.
  • With low molecular weight crosslinking partners, such as Ca ions for example, completely crosslinked spheres can be produced from alginate, which immobilize the microorganisms in the crosslinked structure.
  • Hollow spheres are also known To produce, for example, surround a liquid core, which may contain the crosslinking partner. It is also known to manufacture a membrane by interfacial polymerization of an oil-in-water or water-in-oil emulsion, in which the aqueous phase with a first polymerization partner and the oil phase is provided with a second polymerization partner, so that a membrane is formed at the interfaces
  • the invention is based on the problem of finding a way for a practical application of biological pesticides despite the existing problems.
  • a method of the type mentioned at the outset is characterized according to the invention in that the microorganisms are enclosed within a crosslinked outer shell together with a nutrient medium and in that the outer shell is designed such that the microorganisms first grow inside the outer shell and then escape to the outside.
  • the microorganisms are thus immobilized in a hollow structure, preferably a hollow sphere, a nutrient medium for the microorganisms being enclosed within the hollow structure with the microorganisms.
  • the immobilisate acts as a reactor for the multiplication of the microorganisms.
  • the microorganisms can form a stable and viable culture before they have formed so much biomass that they emerge from the outer shell, for example by destroying the outer shell by the pressure generated by the increasing biomass, or preferably by growing out through the Outer shell through.
  • only a small initial concentration of microorganisms is enclosed with the nutrient medium and the outer shell is designed in such a way that it emerges from the outer shell only after the microorganisms have multiplied by at least a factor of 100.
  • the method according to the invention is therefore based on the fact that the introduction of sufficient biomass for pest control purposes, which was hitherto regarded as critical, does not take place in that the microorganisms are at a higher level
  • the microorganisms immobilized in numerous very small beads may be expedient to enclose the microorganisms immobilized in numerous very small beads to form a cross-linked carrier structure within the outer shell.
  • This is particularly advantageous if at least one seed is also enclosed in the outer casing.
  • a system is created that prevents the infestation of seeds with pests.
  • the beads can be attached to the seed and then enclosed together with the nutrient medium in the outer shell.
  • Beads are formed so small (of the order of 50 ⁇ m in diameter) that the interaction with the nutrient medium is not hindered.
  • the seed can preferably be pearlized with the outer shell by soaking the seed with a first, preferably low molecular weight crosslinking partner for the outer shell and then surrounding it together with the microorganisms with a second crosslinking partner
  • moisturizer in the immobilisate in addition to the microorganisms and the nutrient medium
  • an immobilizate for biological pest control consists, according to the invention, of a cross-linked outer shell in which microorganisms are enclosed together with a nutrient medium
  • the immobilizate can additionally contain at least one seed
  • the microorganisms can be enclosed within the crosslinked outer shell within a multiplicity of spheres from a crosslinked carrier structure.
  • the spheres can adhere to the seed
  • the outer shell can be formed directly on the seed with the microorganisms adhering to it and an adhering nutrient medium
  • the outer shell is preferably formed from at least one polyelectrolyte
  • Particularly suitable polyelectrolytes are alginates because of the mild crosslinking conditions, in particular with Ca ions, preferably in the form of CaCl2 or celluloses, for example carboxyalkyl celluloses, in particular carboxymethyl celluloses, or cellulose ethers containing sulfoalkyl groups, in particular a sulfoalkyl cellulose, preferably sulfoethyl cellulose
  • SEC Sulfoethyl ether
  • SPC sulfopropyl ether
  • mixed celluloses such as hydropropylsulfoalkyl celluloses (e.g. HPSEC, HPSPC), hydroxyethyl alkyl celluloses (e.g. HESEC, HESPC) and carboxymethylsulfoalkyl celluloses (e.g. CMSEC, CMSPC)
  • HPSEC hydropropylsulfoalkyl celluloses
  • HPSEC HPSEC
  • HPSPC hydroxyethyl alkyl celluloses
  • HESEC hydroxyethyl alkyl celluloses
  • HESPC hydroxyethyl alkyl celluloses
  • CMSEC carboxymethylsulfoalkyl celluloses
  • PDMDAAC polydimethyldiallylammonium chloride
  • Alginate is preferably used for the formation of the small spheres enclosing the microorganisms within the crosslinked outer shell.
  • immobilization systems such as carrageenan, polyvinyl alcohol, etc., can also be used
  • alginate or sulfoethyl cellulose has the advantage of being at least largely biodegradable
  • the included nutrient medium naturally depends on the included microorganisms.
  • fungal cultures such as Hirsutella rhossi ensis
  • maize flour or corn glue is particularly suitable, preferably with the addition of a small amount of yeast concentrate
  • Diatomaceous earth, bentonite, polyacrylate, Seramis or the like can be used as moisture stabilizers
  • the dropping time was 1 hour and 32 minutes.
  • the balls were left in the solution for 15 minutes for post-crosslinking.
  • the balls could then be sieved and washed with deionized autoclaved water.
  • the balls can be dried under a sterile bench and stored in a dry state, but also several in the fresh state Stored for days in autoclaved tap water at 6 ° C.
  • the diameter of the balls was 1.3 to 1.4 mm
  • the spheres formed are hollow spheres, since the PDMDAAC solution can only penetrate the SEC solution to a certain degree and the further penetration is prevented by the networked SEC outer shell
  • Figures IA, 1B a first embodiment of an arrangement of microorganisms with a nutrient medium and a seed in a cross-linked outer shell in the state of manufacture and after growing out by propagation
  • FIG. 1 2A 2A representations according to FIG. 1 for a second exemplary embodiment of an arrangement according to FIG. 1
  • Figures 3A 3B representations according to Figure 1 for an arrangement of microorganisms with a nutrient medium within a shell free of microorganisms
  • FIGS. 4A, 4B representations according to FIG. 3 for an arrangement in which microorganisms are located within the networked envelope surrounding a nutrient medium.
  • FIG. 1A shows an immobilizate with a crosslinked outer shell in the form of a thin membrane 1.
  • Microorganisms 2 which move in a nutrient medium 3 are enclosed in the membrane 1.
  • a seed 4 which is protected against pests by the microorganisms 2.
  • the microorganisms can be present in the nutrient medium 3 as such or in a pre-immobilized form.
  • the pre-immobilized form can be carried out by enclosing the microorganisms in a small bead, the inclusion in a Ca-alginate solid sphere preferably being considered.
  • FIG. 1B shows the multiplication of the microorganisms 2 which are stimulated to strong growth due to the nutrient medium 3 and which are removed by the membrane 1. grow, so that considerable amounts of biomass also arise outside the membrane 1
  • FIG. 2A shows an arrangement in which an outer shell 1 ′ is formed by a networked medium in which the microorganisms 2 are located in the core area of the
  • Outer shell 1 'contains the seed 4 and the nutrient medium 3 which surrounds the seed 4 as a thin layer
  • the microorganisms 2 also grow out of the outer casing 1 'in the manner shown in FIG. 2B
  • Figure 3A shows an arrangement with a stable outer shell 1 ", which is free of microorganisms in the illustrated embodiment. Inside the outer shell 1" is the nutrient medium 3 with the microorganisms 2. Here, too, the microorganisms 2 grow out through the outer shell 1 ", as this shows Figure 3B
  • the microorganisms 2 are immobilized within the networked outer shell 1 ", which otherwise corresponds to the outer shell 1" according to FIG. 3A.
  • the nutrient medium 3 In the core of the outer casing 1 ′′ is the nutrient medium 3, by which the microorganisms 2 are stimulated to grow strongly and grow out of the outer casing 1 ′′ according to FIG. 4B
  • a strong growth of the microorganisms 2 is initiated within the immobilizate, so that a large amount of biomass is already present before the microorganisms grow out of the immobilisate, so that the microorganisms 2 are far more sensitive to toxic or mechanical influences from the outside are better protected than would be the case with microorganisms freely applied to a plant or a seed

Abstract

Afin de lutter par voie biologique contre les phytoparasites, au moyen de micro-organismes détruisant les phytoparasites, les micro-organismes sont enfermés dans une gaine extérieure réticulée, avec un milieu nutritif, ladite gaine extérieure étant conçue de façon à pouvoir être détruite ou rendue perméable sous l'effet de la pression des micro-organismes se multipliant à l'intérieur de cette gaine.
PCT/EP1997/004080 1996-08-08 1997-07-28 Procede et immobilisat pour lutter contre les phytoparasites WO1998006267A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU43779/97A AU4377997A (en) 1996-08-08 1997-07-28 Method and immobilizate for the control of plant pests
JP50933398A JP2001504445A (ja) 1996-08-08 1997-07-28 植物有害生物の抑制方法および固定化物
EP97941898A EP0934001A1 (fr) 1996-08-08 1997-07-28 Procede et immobilisat pour lutter contre les phytoparasites

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19632031.3 1996-08-08
DE19632031A DE19632031C1 (de) 1996-08-08 1996-08-08 Verfahren und Immobilisat zur Bekämpfung von Pflanzenschädlingen

Publications (2)

Publication Number Publication Date
WO1998006267A1 true WO1998006267A1 (fr) 1998-02-19
WO1998006267A9 WO1998006267A9 (fr) 1998-05-07

Family

ID=7802138

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1997/004080 WO1998006267A1 (fr) 1996-08-08 1997-07-28 Procede et immobilisat pour lutter contre les phytoparasites

Country Status (6)

Country Link
EP (1) EP0934001A1 (fr)
JP (1) JP2001504445A (fr)
AU (1) AU4377997A (fr)
DE (1) DE19632031C1 (fr)
TR (1) TR199900258T2 (fr)
WO (1) WO1998006267A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3827078A4 (fr) * 2018-07-25 2022-05-11 Lavie Bio Ltd. Micro-organismes encapsulés et leurs procédés d'utilisation

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668512A (en) * 1985-03-20 1987-05-26 The United States Of America As Represented By The Secretary Of Agriculture Preparation of pellets containing fungi and nutrient for control of soilborne plant pathogens
EP0494802A1 (fr) * 1991-01-03 1992-07-15 Lipha, Lyonnaise Industrielle Pharmaceutique Graines revêtues de préparations déshydratées de microorganismes et leur procédé d'obtention
WO1992020229A1 (fr) * 1991-05-23 1992-11-26 Korea Research Institute Of Chemical Technology Procede ameliore de preparation de pesticides microbiens enrobes et pesticides obtenus selon ce procede
EP0526800A1 (fr) * 1991-08-03 1993-02-10 BASF Aktiengesellschaft Polymères de composés contenant de l'azote polymérisés en présence de monosaccharides, oligosaccharides et polysaccharides et leurs dérivés
WO1994028725A1 (fr) * 1993-06-16 1994-12-22 Incitec Ltd. Composition de champignons nematophages

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5358863A (en) * 1993-03-30 1994-10-25 The United States Of America As Represented By The Secretary Of The Agriculture Oil and absorbent coated granules containing encapsulated living organisms for controlling agricultural pests

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668512A (en) * 1985-03-20 1987-05-26 The United States Of America As Represented By The Secretary Of Agriculture Preparation of pellets containing fungi and nutrient for control of soilborne plant pathogens
EP0494802A1 (fr) * 1991-01-03 1992-07-15 Lipha, Lyonnaise Industrielle Pharmaceutique Graines revêtues de préparations déshydratées de microorganismes et leur procédé d'obtention
WO1992020229A1 (fr) * 1991-05-23 1992-11-26 Korea Research Institute Of Chemical Technology Procede ameliore de preparation de pesticides microbiens enrobes et pesticides obtenus selon ce procede
EP0526800A1 (fr) * 1991-08-03 1993-02-10 BASF Aktiengesellschaft Polymères de composés contenant de l'azote polymérisés en présence de monosaccharides, oligosaccharides et polysaccharides et leurs dérivés
WO1994028725A1 (fr) * 1993-06-16 1994-12-22 Incitec Ltd. Composition de champignons nematophages

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3827078A4 (fr) * 2018-07-25 2022-05-11 Lavie Bio Ltd. Micro-organismes encapsulés et leurs procédés d'utilisation
US11718842B2 (en) 2018-07-25 2023-08-08 Lavie Bio Ltd. Encapsulated microorganisms and methods of using same

Also Published As

Publication number Publication date
AU4377997A (en) 1998-03-06
TR199900258T2 (xx) 1999-06-21
JP2001504445A (ja) 2001-04-03
DE19632031C1 (de) 1998-01-08
EP0934001A1 (fr) 1999-08-11

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