WO1997037039A3 - Verwendung extrem thermophiler dna-polymerasen - Google Patents

Verwendung extrem thermophiler dna-polymerasen Download PDF

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Publication number
WO1997037039A3
WO1997037039A3 PCT/EP1997/001589 EP9701589W WO9737039A3 WO 1997037039 A3 WO1997037039 A3 WO 1997037039A3 EP 9701589 W EP9701589 W EP 9701589W WO 9737039 A3 WO9737039 A3 WO 9737039A3
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WO
WIPO (PCT)
Prior art keywords
polymerases
extremely thermophilic
thermophilic dna
dna
extremely
Prior art date
Application number
PCT/EP1997/001589
Other languages
English (en)
French (fr)
Other versions
WO1997037039A2 (de
Inventor
Juergen Blusch
Original Assignee
Gsf Forschungszentrum Umwelt
Juergen Blusch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gsf Forschungszentrum Umwelt, Juergen Blusch filed Critical Gsf Forschungszentrum Umwelt
Priority to EP97915455A priority Critical patent/EP0904404A2/de
Priority to US09/155,512 priority patent/US6251637B1/en
Priority to JP53492797A priority patent/JP3378255B2/ja
Publication of WO1997037039A2 publication Critical patent/WO1997037039A2/de
Publication of WO1997037039A3 publication Critical patent/WO1997037039A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Erfindungsgemäß wird ein Verfahren zur Zyklus-Sequenzierung von DNA mit Dye-Terminatoren offenbart, wobei extrem thermophile DNA-Polymerasen bei einer Kettenverlängerungstemperatur im Bereich von > 60 °C bis 80 °C verwendet werden. Weiterhin wird eine Verwendung extrem thermophiler DNA-Polymerasen bei Temperatur im Bereich von > 60 °C bis 80 °C offenbart.
PCT/EP1997/001589 1996-03-29 1997-03-27 Verwendung extrem thermophiler dna-polymerasen WO1997037039A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP97915455A EP0904404A2 (de) 1996-03-29 1997-03-27 Verwendung extrem thermophiler dna-polymerasen
US09/155,512 US6251637B1 (en) 1996-03-29 1997-03-27 Use of extremely thermophilic DNA-polymerases
JP53492797A JP3378255B2 (ja) 1996-03-29 1997-03-27 超好熱性ｄｎａポリメラーゼの使用

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19612684.3 1996-03-29
DE19612684A DE19612684A1 (de) 1996-03-29 1996-03-29 Neue Verwendung extrem thermophiler DNA-Polymerasen

Publications (2)

Publication Number Publication Date
WO1997037039A2 WO1997037039A2 (de) 1997-10-09
WO1997037039A3 true WO1997037039A3 (de) 1997-11-27

Family

ID=7789948

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1997/001589 WO1997037039A2 (de) 1996-03-29 1997-03-27 Verwendung extrem thermophiler dna-polymerasen

Country Status (5)

Country Link
US (1) US6251637B1 (de)
EP (1) EP0904404A2 (de)
JP (1) JP3378255B2 (de)
DE (1) DE19612684A1 (de)
WO (1) WO1997037039A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9807045D0 (en) * 1998-04-01 1998-06-03 Rudi Knut Nucleic acid detection method
EP1343371A4 (de) * 2000-09-08 2004-08-04 Invitrogen Corp Zusammensetzungen und verfahren für die verbesserung der empfindlichkeit und spezifität bei der nukleinsäuresynthese
WO2005024053A1 (en) 2003-09-04 2005-03-17 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
US7833942B2 (en) * 2004-12-03 2010-11-16 Human Genetic Signatures Pty. Ltd. Methods for simplifying microbial nucleic acids by chemical modification of cytosines
JP2008541705A (ja) * 2005-05-26 2008-11-27 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 非標準塩基を含むプライマーを使用する等温鎖置換増幅
US8343738B2 (en) * 2005-09-14 2013-01-01 Human Genetic Signatures Pty. Ltd. Assay for screening for potential cervical cancer
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
JP2010521142A (ja) * 2007-03-16 2010-06-24 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 遺伝子発現アッセイ
WO2009067743A1 (en) * 2007-11-27 2009-06-04 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
EP2222850A4 (de) * 2007-12-20 2011-12-07 Human Genetic Signatures Pty Beseitigung von kontaminanten in zusammenhang mit nukleinsäureamplifikation
EP2333109B1 (de) * 2008-09-03 2013-08-07 Takara Bio, Inc. Zusammensetzung für den nachweis von rna
MX350658B (es) 2011-09-07 2017-09-13 Human Genetic Signatures Pty Ltd Ensayo de detección molecular.

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0409078A2 (de) * 1989-07-19 1991-01-23 BASF Aktiengesellschaft Verfahren zur Sequenzierung von Desoxyribonukleinsäuren
EP0655506A1 (de) * 1994-10-17 1995-05-31 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4782137A (en) * 1984-01-24 1988-11-01 Immunex Corporation Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same
US5489523A (en) * 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0409078A2 (de) * 1989-07-19 1991-01-23 BASF Aktiengesellschaft Verfahren zur Sequenzierung von Desoxyribonukleinsäuren
EP0655506A1 (de) * 1994-10-17 1995-05-31 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ELLINGBOE AND GYLLENSTEN: "PCR Technique: DNA Sequencing", 1992, EATON PUBLISHING, NATICK, US, XP002041635, 200190 *
KRETZ K ET AL: "CYCLE SEQUENCING", PCR METHODS & APPLICATIONS, vol. 3, no. 5, April 1994 (1994-04-01), pages S107 - S112, XP000606764 *
MULLIS ET AL.: "The Polymerase Chain Reaction", 1994, BIRKHAUSER, BOSTON, US, XP002041636, 206150 *
SAMBROOK ET AL.: "Molecular cloning", 1987, COLD SPRING HARBOUR PRESS, NY, US, XP002041637, 166120 *

Also Published As

Publication number Publication date
WO1997037039A2 (de) 1997-10-09
JP2000503214A (ja) 2000-03-21
JP3378255B2 (ja) 2003-02-17
EP0904404A2 (de) 1999-03-31
US6251637B1 (en) 2001-06-26
DE19612684A1 (de) 1997-10-02

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