WO1997014310A1 - Preparations fongicides a base d'hydrates de carbone - Google Patents

Preparations fongicides a base d'hydrates de carbone Download PDF

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Publication number
WO1997014310A1
WO1997014310A1 PCT/DK1996/000440 DK9600440W WO9714310A1 WO 1997014310 A1 WO1997014310 A1 WO 1997014310A1 DK 9600440 W DK9600440 W DK 9600440W WO 9714310 A1 WO9714310 A1 WO 9714310A1
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Prior art keywords
composition according
examples
enzyme
fungi
saccharide
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PCT/DK1996/000440
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English (en)
Inventor
Robertus Fleuren
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Novo Nordisk A/S
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Priority to AU72794/96A priority Critical patent/AU7279496A/en
Publication of WO1997014310A1 publication Critical patent/WO1997014310A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

Definitions

  • the present invention relates to the use of saccharides as enhancers of biocidal agents, compositions comprising said saccharides, and methods for combating pests by applying said compositions.
  • enzymes may enhance the biocidal effect of synthetic chemical biocides in a synergistic way. This includes enzymes such as proteases, esterases, cellulases, hemicellulases, lipases, pectinases, and endo chitinases.
  • enzymes such as proteases, esterases, cellulases, hemicellulases, lipases, pectinases, and endo chitinases.
  • Target Sites of Fungicide Action CRC Press, Boca Raton, Florida, 1992, at pages 313-314 reviews number of disclosures directed to synergistic interaction of different lytic enzymes produced by a variety of microorganisms with a small number of antifungal compounds including amphotericin B, benomyl, polyoxin B, kitazin P and nikkomycin.
  • EP 197622 Korean University
  • EP 272002 Korean University
  • EP 272002 Korean University
  • EP 272002 Korean University
  • WO 94/13784 (Cornell University)
  • WO 94/13784 discloses the antifungal synergistic combination of an enzyme fungicide and a non-enzymatic fungicide and the use thereof.
  • the disclosed enzymes are glucanolytic enzymes and cellulases (EC 3.2.1.4) from Trichoderma harzianum.
  • the disclosed non-enzymatic fungicides are selected from the group consisting of sterol synthesis inhibiting fungicides (e.g. flusilazole and miconazole) and thiol group inactivating fungicides. 5 WO 94/24271 (Cornell Res. Fdn. Inc), published 27.06.1994 further discloses inhibition of germination, replication or growth by use of chitin and b-l,3-glucan containing fungus.
  • the antifungal composition contains endo chitinase, chitin-1,4-b- chitobiosidase, N-acetyl-b-glucosidase isolated from Trichoderma o harzianum strain Pl .
  • saccharides as stabilising agents of proteins, especially enzymes, is well known.
  • DE 2038103 (Henkel and Cie) 0 published august 1972 discloses the use of monosaccharide and disaccharide alcohols as enzyme stabilising agents in dishwashing compositions.
  • JP 760903 (Meito Sangyo) published 03.09.1976 discloses monosaccharides and oligosaccharides as enzyme stabilisers.
  • 25 Saccharides as growth enhancer of plants is disclosed in JP 1117807 (Mitsui & Co) published 10.05.1987. Here, glucose and fructose is sprayed directly on the leaves of plants with a growth promoting effect. No biocidal effect is disclosed.
  • the invention relates to a fungicidal composition
  • a fungicidal composition comprising a saccharide in combination with a biocidally active preparation in admixture with a fungicidally acceptable diluent or carrier.
  • composition according to the invention may further comprise an enzymatic composition, being either an enzyme, an enzyme preparation, or an enzyme complex.
  • the invention furthermore relates to the use of saccharides for enhancing the fungicidal effect of a fungicide.
  • the invention also relates to a method of controlling fungi by application of a composition of the invention.
  • Figure 1t Visualises the evaluation system ranking plants from 0 to 9 depending on the damage from the pest.
  • the illustrated pest is Phytopthora infestans on potato plants.
  • Figure 2 Illustrates four tomato plants inoculated with Phytopthora infestans: From left to right. Number one is treated with 100 ppm Mancozeb and 1% Celluclast ® , number two is treated with 100 ppm Mancozeb, the third is untreated, and the fourth is treated with 1 % Celluclast ® .
  • Celluclast ® was maintained after autoclavation. In order to examine this phenomenon, tests were conducted with the additives in the Celluclast formulation: Saccharides and salts.
  • Saccharides and salts were conducted with the additives in the Celluclast formulation: Saccharides and salts.
  • the present invention discloses for the first time the use of saccharides as enhancers of biocides to obtain improved pest control.
  • the present invention comprises a 5 composition of a saccharide in combination with a biocidally active preparation in admixture with a biologically acceptable diluent or carrier.
  • Said saccharide comprises monosaccharides, disaccharides, polyols, or polypeptides, and salt or acids of said groups.
  • Said o monosaccharides comprises ribose, arabinose, xylose, glucose, fructose, mannose, or galactose
  • said disaccharides comprises sucrose, lactose, maltose, or cellobiose
  • said polyols comprises sorbitol, mannitol, galactitol, ribitol, allitol, or xylitol
  • said polysaccharides comprises cellulose, pectin, algin, inulin, 5 amylose, or dextrose and said acids comprises gluconic acid, mannonic acid, or arabinonic acid.
  • the concentration of the saccharide in the composition of the present invention as applied to plants is preferably within the range of 0.001 to 70 vol.%. 20 It may be 0.001%, 0.01%, 0.05%, 0.1%, 0.3%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 9.5%, 10.5%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 25 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%,
  • the amount of saccharide can vary widely and be, for example, from 5 to 90 percent of the volume.
  • a facilitating effect 30 of the interaction between a lytic enzyme complex and a biocidally active compound or composition is often obtained by adding a saccharide. This facilitating effect allows application of both enzyme and the other biocide in considerably lower dosages than the ones usually applied, while obtaining the same 35 or improved control effect of the pest.
  • the enzymatic composition comprises either an enzyme, an enzyme preparation, or an enzyme complex. Said enzymatic composition may further comprise a mixture of enzymes or enzyme activities .
  • said enzyme preparation comprises one or more enzymes selected from the group comprising cellulases or hemicellulases . Said enzyme preparation further being a microbial, e.g. bacterial or fungal origin.
  • said enzyme comprises Celluclast ® or other cellulytic compositions containing saccharides.
  • said enzyme preparation comprises enzymes that are substantially composed of one enzyme activity (monocomponent enzymes) .
  • said monocomponent enzyme comprises Xylanase I, Xylanase II and Endoglucanase III.
  • the concentration of said enzyme lies in the range from 0.001 to 5 w/w, preferably in the range from 0.01 to 2% w/w, more preferably in the range from 0.05 to 1.5%w/w, even more preferably between 0.1 to 1.0%w/w.
  • the invention is applicable to fungi, and the invention thus also provides a method of combating fungi at a locus infested or liable to be infested therewith, which comprises applying to the locus a fungicidally active preparation of the invention, and in a preferred embodiment of the invention said locus is subjected sequentially or in a mixture to the saccharide preparation of this invention as well as said other biocide.
  • the invention provides a composition comprising a selected saccharide and a fungicide in admixture with an acceptable diluent or carrier.
  • composition of the invention in addition to said fungicidally active compounds may also contain other active ingredients, e.g. pesticides, such as further fungi ⁇ cides, herbicides, nematocides, rodenticides, bactericides, molluscicides or acaricides, or plant nutrients or fertilisers.
  • pesticides such as further fungi ⁇ cides, herbicides, nematocides, rodenticides, bactericides, molluscicides or acaricides, or plant nutrients or fertilisers.
  • Examples of synthetic fungicides which can be combined with the saccharide preparation of the invention either alone or in combination with an enzyme include the acetamides, examples of which are the common names cy ⁇ noxanil and ofurace; the acylalanines, examples of which are the common names benalaxyl and etalaxyl; the amines, of which examples are the common names 2-aminobutane and diphenylamine; the anilides, examples of which are the common names benodanil and flutalonil; the antibiotics, of which examples are the common names blasticidin-S and polyoxins; the benzimidazoles, of which examples are the common names benomyl and thiabendazole; the carbamates, of which examples are the common names diethofencarb and propamocarb; the conazoles, of which examples are the common names prochloraz and propiconazole; the dicarboximides, of which examples are the common names iprodione and vinclo
  • the synthetic fungicide may preferably be chosen from the group comprising anilides, alanines, carbamates, azoles, dicarboximides, phthalimides, amides and organophosphorous derivatives, more preferably from the group comprising carbamates, azoles, and phtalimides.
  • Said fungicide could be mancozeb, aneb, chlorothalonil, or propicinazol.
  • Examples of synthetic herbicides which can be combined with the saccharide preparation of the invention in combination with an enzyme include the acetamides, of which examples are the common names acetochlor and diphenamid; the amides, of which examples are the common names propyzamide and tebutam; the anilides, of which examples are the common names bromobutide and mefenacet; the aryloxylakanoic acids, of which examples are the common names dichlorprop and mecoprop; the auxins, of which examples are the common names benazolin and picloram; the bipyridyls, of which examples are the common names diquat and paraquat; the bridged diphenyls, of which examples are the common names bifenox and diclofop; the carbamates, of which examples are the common names asulam and carbetamide; the chloroacetanilides, of which examples are the common names acetochlor and pretilachlor,* the dinitroanilines, of which
  • the synthetic herbicides may preferably be chosen from the groups comprising phosphinic acids, phenoxy derivatives and sulfonylureas. Said herbicide could be glyphosate, fluazifop -p- butyl, and tribenuronmethyl.
  • Examples of synthetic nematocides which can be combined with the saccharide preparation of the invention in combination with an enzyme include the carbamates, of which examples are the common names aldicarb and carbofuran; the organochlorine derivatives, of which examples are the common names chloropicrin and DCIP; the organophosphorous derivatives, of which examples are the common names cadusafos and phorate; and miscellaneous compounds, including dazomet, fosthiazate, methyl isothiocyanate, and oxamyl .
  • Examples of synthetic rodenticides which can be combined with the saccharide preparation of the invention in combination with an enzyme include the coumarins, examples of which are the common names brodifacoum and warfarin; the indandiones, of which examples are the common names diphacinone and pindone; the inorganics, examples of which are the common names hydrogen cyanide and zinc phosphide; the secosteroids, of which examples are the common names calciferol and cholecalciferol; and miscellaneous compounds including bisthiosemi, bromethalin, chloralose, chlorophacinone, difethialone, flouroacteamide, flupropadine, scilliroside, sodium fluoroacetate, and strychnine.
  • Examples of synthetic bactericides which can be combined with the saccharide preparation of the invention in combination with an enzyme include the antibiotics, of which examples are the common names kasugamycin and streptomycin; the quinolines, examples of which are the common names 8-hydroxyquinoline sulfate and oxolonic acid; and miscellaneous compounds including, bronopol, copper hydroxide, dichlorophen, formaldehyde, nickel dimethyldithiocarbamate, nitrapyrin, octhilinone, probenazole and tecloftalam.
  • antibiotics of which examples are the common names kasugamycin and streptomycin
  • the quinolines examples of which are the common names 8-hydroxyquinoline sulfate and oxolonic acid
  • miscellaneous compounds including, bronopol, copper hydroxide, dichlorophen, formaldehyde, nickel dimethyldithiocarbamate, nitrapyrin, octhilinone,
  • Examples of synthetic molluscicides which can be combined with the saccharide preparation of the invention in combination with an enzyme include the carbamates, of which examples are the common names methiocarb and thiodicarb; and miscellaneous compounds including, fentin acetate, metaldehyde, and niclosamide.
  • Examples of synthetic acaricides which can be combined with the saccharide preparation of the invention in combination with an enzyme include the carbamates, of which examples are the common names aldicarb and methiocarb; the bridged dipenhyls, of which examples are the common names chlorobenzilate and dicofol; the carbamoyloximes, of which examples are the common names aldoxycarb and oxamyl; the dinintrophenyls, of which examples are the common names dinocap and DNOC; the organochlorine, of which examples are the common names dienochlor and tetradifon; the organophosphourous derivatives, of which examples are the common names malathion and parathion; the organotins, of which examples are the common names azocyclotin and SSI-121; the phtalimides, of which examples are the common names dialifos and phosmet; the pyrimidines, of which examples are the common names diazinon and
  • the concentration of the biocide lies in the range from 0.001 to70% w/w.
  • the diluent or carrier in the compositions of the invention can be a solid or a liquid optionally in association with a surface-active agent, for example a dispersing agent, emulsifying agent or wetting agent.
  • Suitable surface-active agents include anionic compounds such as a carboxylate, for example a metal carboxylate of a long chain fatty acid; an N-acylsarcosinate; mono- and di-esters of phosphoric acid with fatty alcohol ethoxylates or salts of such esters; fatty alcohol sulphates such as sodium dodecyl sulphate, sodium octadecyl sulphate or sodium cetyl sulphate; ethoxylated fatty alcohol sulphates; ethoxylated alkylphenol sulphates; lignin sulphonates; petroleum sulphonates; alkyl-aryl sulphonates such as alkyl-benzene sulphonates or lower alkyl
  • butyl-naphthalene sulphonate salts of sulphonated naphthalene-formaldehyde condensates; salts of sulphonated phenol-formaldehyde condensates; or more complex sulphonates such as the amide sulphonates, e.g. the sulphonated condensation product of oleic acid and N-methyl taurine or the dialkyl sulphosuccinates, e.g. the sodium sulphonate of dioctyl succinate .
  • amide sulphonates e.g. the sulphonated condensation product of oleic acid and N-methyl taurine or the dialkyl sulphosuccinates, e.g. the sodium sulphonate of dioctyl succinate .
  • Nonionic agents include condensation products of fatty acid esters, fatty alcohols, fatty acid amides or fatty-alkyl- or alkenyl-substituted phenols with ethylene oxide, fatty esters of polyhydric alcohol ethers, e.g. sorbitan fatty acid esters, condensation products of such esters with ethylene oxide, e.g. polyoxyethylene sorbitan fatty acid esters, block copolymers of ethylene oxide and propylene oxide, acetylenic glycols such as 2,4, 7, 9-tetraethyl-5-decyn-4, 7-diol, or ethoxylated acetylenic glycols.
  • a cationic surface-active agent examples include, for instance, an aliphatic mono-, di-, or polyamine as an acetate, naphthenate or oleate; an oxygen-containing amine such as an amine oxide or polyoxyethylene alkylamine; an amide-linked amine prepared by the condensation of a carboxylic acid with a di- or polyamine; or a quaternary ammonium salt.
  • the diluent or carrier in the composition of the invention may be a solid or a liquid conventionally used for the purpose.
  • solid carriers bentonite diatomaceous earth, apatite, gypsum, talc, pyrophyllite, vermiculite, ground shells, and clay may be mentioned.
  • composition of the invention can be in any form known in the art for the formulation of pesticides, for example, an emulsifiable concentrate, a concentrated emulsion, a multiple emulsion, an aqueous emulsion, a solution, a dispersion, a sus ⁇ pension concentrate, a release formulation (including a slow release formulation) , a seed dressing, a granular formulation, a water soluble powder, a wettable powder, a dusting powder, a dispersible powder, an alginate, a xanthan gum and/or an aerosol.
  • it can be in a suitable form for direct application or as a concentrate or primary composition which requires dilution with a suitable quantity of water or other diluent before application.
  • An emulsifiable concentrate comprises the active ingredient and the saccharide of the invention dissolved in a water- immiscible solvent which is formed into an emulsion with water in the presence of an emulsifying agent .
  • Another suitable concentrate is a flowable suspension concentrate which is formed by grinding the active ingredient and with water or other liquid, a wetting agent and a suspending agent.
  • a dusting powder comprises the active ingredient and intimately mixed and ground with a solid pulverulent diluent, for example, kaolin.
  • a granular solid comprises the active ingredient and associated with similar diluents to those which may be employed in dusting powders, but the mixture is granulated by known methods. Alternatively it comprises the active ingredient and absorbed or adsorbed on a pre-granular diluent for example, Fuller's earth, attapulgite or limestone grit.
  • Wettable powders, granules or grains usually comprise the active ingredient and in admixture with a suitable surfactant and an inert powder diluent such as china clay.
  • concentration of the active compounds of the invention described herein in the compositions of the invention may vary within a wide range depending on the type of formulation and the field of application.
  • the concentration of the saccharide in the compositions of the present invention when used in combination with a conventional biocide, as applied to pests is preferably within the range from 0.001 to 10 per cent by weight, especially 0.03 to 5 per cent by weight.
  • the amount of saccharide can vary widely and can be, for example, in the range from about 5 to about 90 per cent by weight of the composition.
  • the preparation or the compositions of the invention with saccharide can be applied directly to the pest by, for example, spraying or dusting.
  • the saccharide in combination with a conventional fungicide can also be applied to seeds or habitat.
  • the preparation can be applied directly to the soil before, at or after drilling so that the presence of active ingredient in the soil can control the growth of pests which may attack or influence seeds or seedlings.
  • the saccharide in admixture with the conventional biocide can be applied in any manner which allows it to be intimately mixed with the soil, such as by spraying, by broadcasting a solid form of granules, or by applying the active ingredient and at the same time as drilling by inserting it in the same drill as the seeds.
  • the invention is further illustrated with reference to the following examples which are not intended to be in any way limiting to the scope of the invention as claimed.
  • Detached potato leaves are placed with the dorsal side upwards in a non-covering formation on nets in plastic containers, containing filter paper moistened with sterile water.
  • Sporangia from infected leaves are washed with 5°C cold phosphate-calcium buffer (DS-buffer, half strength) , filtered through 15 mm silk gauze, and rinsed several times with cold DS- buffer to remove bacteria.
  • the sporangia left on the silk gauze are transferred to a clean beaker, and the concentration is determined and adjusted to 0.5 - 1.0 x IO 5 spores ml "1 .
  • Each tomato plant is sprayed with 6-8 ml spore suspension (all leaves on both dorsal and ventral sides) , ensuring that the plants are covered homogeneously.
  • the exact volume used on each plant is calculated by measuring the remaining spore suspension.
  • the pots are placed in sealed plexi glass boxes in trays containing 0.5 1 tap water, to ensure a high level of humidity, under the following conditions: Light: 10,000 lux (minus the first 24 hours) 16 hours/day, temperature: 18°C day and 15°C night.
  • the leaves of the barley plants are fixed onto a horizontal net, ensuring a homogenous infection of all leaves, and all pots are placed in trays at the bottom of the inoculation tower.
  • a pressure of 0.5 bar for 30 seconds the conidia are dispersed from the top of the tower. After 20 minutes, the exact inoculum pressure is determined by spore count on object glasses placed 5 different places in the bottom of the tower.
  • the pots are placed in growth compartments with the following conditions: Light: minimum 5000 lux 16 hours/day, temperature: 20°C day/night .
  • the wheat plants are sprayed with 2-4 ml spore suspension, ensuring that the plants are uniformly covered.
  • the exact volume used on each plant is calculated by measuring the remaining spore suspension.
  • the pots are kept dark in trays in metal containers with M- 1 liter water added, under the following conditions: Temperature: 17°C day, 15°C night. After 24 hours, the trays are moved to a growth compartment under the following conditions: Light: minimum 5000 lux 16 hours/day, temperature: 20°C day/night . Assessment
  • Puccinia recondi ta 9-12 days after inoculation, depending on disease severity (criterion - inoculated untreated plants totally infected) , by the following scale:
  • Celluclast ® composition Enzymatic proteins within the groups cellulases and hemicellulases and other proteins. Additives for stabilization are Sorbitol and salt.
  • results from the tests are expressed as mean values and ranked in SNK-groupings.
  • - N is the number of plants.
  • the concentration of the fungicide (F) Mancozeb is 100 ppm if nothing else is mentioned.
  • Sorbitol concentration of Sorbitol is 0.3 vol% pr mix. if nothing else is mentioned.
  • SSP Sorbitol, Sodium Chloride and Potassium Sorbate.
  • the cellulytic activity may be determined in endo-cellulase units (ECU) by measuring the ability of the enzyme to reduce the viscosity of a solution of carboxymethyl cellulose (CMC) .
  • ECU endo-cellulase units
  • the ECU assay quantifies the amount of catalytic activity present in the sample by measuring the ability of the sample to reduce the viscosity of a solution of carboxy-methylcellulose (CMC) .
  • the assay is carried out at 40°C; pH 7.5; 0.IM phosphate buffer; time 30 min; using a relative enzyme standard for reducing the viscosity of the CMC Hercules 7 LFD substrate; enzyme concentration approx. 0.15 ECU/ml.
  • the arch standard is defined to 8200 ECU/g.
  • the cellulytic activity may be measured in endo-glucanase units (EGU) , determined at pH 6.0 with carboxymethyl cellulose
  • a substrate solution is prepared, containing 34.0 g/1 CMC
  • EGU is defined as the amount of enzyme that reduces the viscosity to one half under these conditions.
  • the amount of enzyme sample should be adjusted to provide 0.01-0.02 EGU/ml in the reaction mixture.
  • the arch standard is defined as 880 EGU/g. NCU :
  • the cellulytic activity is determined with carboxymethyl cellulose (CMC) as substrate.
  • CMC carboxymethyl cellulose
  • NCU Novo Cellulase Unit
  • One Novo Cellulase Unit (NCU) is defined as the amount of enzyme which, under standard conditions (i.e. at pH 4.80; 0.1 M acetate buffer; 10 g/1 Hercules CMC type 7 LFD as substrate; an incubation temp, of 40.0°C; an incubation time of 20 min; and an enzyme concentration of approximately 0.041 NCU/ml) forms an amount of reducing carbohydrates equivalent to 1 mmol glucose per minute.
  • the xylanolytic activity can be expressed in FXU-units, determined at pH 6.0 with remazol-xylan (4-0-methyl-D-glucurono- D-xylan dyed with Remazol Brilliant Blue R, Fluka) as substrate.
  • a xylanase sample is incubated with the remazol-xylan substrate.
  • the background of non-degraded dyed substrate is precipitated by ethanol .
  • the remaining blue colour in the supernatant (as determined spectrophotometrically at 585 nm) is proportional to the xylanase activity, and the xylanase units are then determined relatively to an enzyme standard at standard reaction conditions, i.e. at 50.0°C, pH 6.0, and 30 minutes reaction time.
  • NCU activity of 0.5 % non-formulated Celluclast ® 22.0 NCU/g.
  • Table 4 shows that an increase in Sorbitol content enhanced the activity of the fungicide. At a concentration of 5%, there were no indications that this was maximum for enhancing activity.
  • Sorbitol was unique at enhancing pesticides
  • other polyols as well as mono- and di-saccharides were examined for enhancing activity on fungicides.
  • the tests were performed with the saccharides at a number of moles equal to that of Sorbitol: 1.6468 mmol.
  • Celluclast ® (implicit Sorbitol) is able to enhance the effect of different types of fungicides: Table 13 demonstrates the same for
  • Propiconazole (trade name Tilt 250 EC) (active against Erysiphe graminis) and Puccinia recondi ta ) . It has also been demonstrated that the enhancing capability is not limited to certain fungi (examples included are Oomycetes
  • the enhancing capability is not limited to certain plants (examples included are mono ⁇ cotyledons such as barley and wheat, and dicotyledons such as potato and tomato) .

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
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  • Engineering & Computer Science (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne l'utilisation de saccharides en qualité d'activateurs d'agents fongicides, ainsi que des compositions comprenant ces saccharides combinés à des agents fongicides, et des procédés de lutte contre des agents pathogènes par application de ces compositions.
PCT/DK1996/000440 1995-10-13 1996-10-14 Preparations fongicides a base d'hydrates de carbone WO1997014310A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU72794/96A AU7279496A (en) 1995-10-13 1996-10-14 Fungicidal carbohydrate preparations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK1160/95 1995-10-13
DK116095 1995-10-13

Publications (1)

Publication Number Publication Date
WO1997014310A1 true WO1997014310A1 (fr) 1997-04-24

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2777423A1 (fr) * 1998-04-16 1999-10-22 Rhone Poulenc Agrochimie Nouvelle utilisation de composes antifongiques et/ou antibacteriens et/ou antiviraux
WO1999053761A1 (fr) * 1998-04-16 1999-10-28 Aventis Cropscience S.A. Nouvelle utilisation de composes antifongiques et/ou antibacteriens et/ou antiviraux
WO2000024255A1 (fr) * 1998-10-26 2000-05-04 Cognis Deutschland Gmbh Utilisation de melanges de matieres valorisables pour stimuler la croissance et ameliorer la sante de plantes
EP1023836A1 (fr) * 1999-01-26 2000-08-02 Unilever N.V. Compositions et procédés pour inhiber la croissance des champignons
US6280725B1 (en) 2000-01-18 2001-08-28 Lipton, Division Of Conopco, Inc. Compositions and methods for inhibiting the growth of fungi
US6734170B2 (en) * 1999-05-17 2004-05-11 Aesgan, Inc. Cellular uptake of bioactive agents
USRE39485E1 (en) 1993-03-30 2007-02-06 Aesgen, Inc. Oral Glutamine to reduce stomatitis
EP1849364A1 (fr) * 2006-04-26 2007-10-31 Basf Aktiengesellschaft Composition comprenant un glucane ou un dérivé de glucane et un pesticide, et procédés d'amélioration de la santé des plantes
US7709529B2 (en) 2002-08-01 2010-05-04 Eisai Inc. Treatment of cancer with glutamine
JP2013523604A (ja) * 2010-03-26 2013-06-17 エムピーティー マスタード プロダクツ アンド テクノロジーズ インコーポレーティッド 植物材料と糖とを含む有害生物の制御のための組成物、および作製方法

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EP0197622A1 (fr) * 1985-01-18 1986-10-15 Kao Corporation Augmentation de l'activité biocide
EP0272002A1 (fr) * 1986-11-19 1988-06-22 Genencor International, Inc. Enzymes comme adjuvants chimiques agricoles
WO1991013552A1 (fr) * 1990-03-06 1991-09-19 David Tate Compositions fongicides destinees a etre appliquees a des plantes
EP0557946A1 (fr) * 1992-02-26 1993-09-01 The Green Cross Corporation Composition contenant l'isothiocyanate allylique et son utilisation
WO1993022912A1 (fr) * 1992-05-12 1993-11-25 Church & Dwight Company Compositions fongicides
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US5395530A (en) * 1993-06-17 1995-03-07 Nalco Chemical Company Treatment of fresh waters to destroy filamentous bacteria
WO1995022253A1 (fr) * 1994-02-17 1995-08-24 Abbott Laboratories Adjuvant de pesticide
DE19506095A1 (de) * 1994-03-04 1995-09-21 Bayer Agrochem Kk Schädlingsbekämpfungsmittel in Pastenform

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE39485E1 (en) 1993-03-30 2007-02-06 Aesgen, Inc. Oral Glutamine to reduce stomatitis
US6770303B1 (en) 1998-04-16 2004-08-03 Bayer Cropscience S.A. Use of antifungal and/or antibacterial and/or antiviral compounds
WO1999053761A1 (fr) * 1998-04-16 1999-10-28 Aventis Cropscience S.A. Nouvelle utilisation de composes antifongiques et/ou antibacteriens et/ou antiviraux
FR2777423A1 (fr) * 1998-04-16 1999-10-22 Rhone Poulenc Agrochimie Nouvelle utilisation de composes antifongiques et/ou antibacteriens et/ou antiviraux
WO2000024255A1 (fr) * 1998-10-26 2000-05-04 Cognis Deutschland Gmbh Utilisation de melanges de matieres valorisables pour stimuler la croissance et ameliorer la sante de plantes
EP1023836A1 (fr) * 1999-01-26 2000-08-02 Unilever N.V. Compositions et procédés pour inhiber la croissance des champignons
US6734170B2 (en) * 1999-05-17 2004-05-11 Aesgan, Inc. Cellular uptake of bioactive agents
US7041651B2 (en) 1999-05-17 2006-05-09 Aesgen, Inc. Cellular uptake of bioactive agents
US6280725B1 (en) 2000-01-18 2001-08-28 Lipton, Division Of Conopco, Inc. Compositions and methods for inhibiting the growth of fungi
US7709529B2 (en) 2002-08-01 2010-05-04 Eisai Inc. Treatment of cancer with glutamine
EP1849364A1 (fr) * 2006-04-26 2007-10-31 Basf Aktiengesellschaft Composition comprenant un glucane ou un dérivé de glucane et un pesticide, et procédés d'amélioration de la santé des plantes
WO2007122264A2 (fr) * 2006-04-26 2007-11-01 Basf Se Composition et procédé permettant d'améliorer la santé des plantes
WO2007122264A3 (fr) * 2006-04-26 2008-10-23 Basf Se Composition et procédé permettant d'améliorer la santé des plantes
JP2013523604A (ja) * 2010-03-26 2013-06-17 エムピーティー マスタード プロダクツ アンド テクノロジーズ インコーポレーティッド 植物材料と糖とを含む有害生物の制御のための組成物、および作製方法

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