WO1997005227A1 - An anionic stabilized enzyme-based clean-in-place system - Google Patents

An anionic stabilized enzyme-based clean-in-place system Download PDF

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Publication number
WO1997005227A1
WO1997005227A1 PCT/US1996/012052 US9612052W WO9705227A1 WO 1997005227 A1 WO1997005227 A1 WO 1997005227A1 US 9612052 W US9612052 W US 9612052W WO 9705227 A1 WO9705227 A1 WO 9705227A1
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WO
WIPO (PCT)
Prior art keywords
enzyme
concentrate
water
weight
part system
Prior art date
Application number
PCT/US1996/012052
Other languages
English (en)
French (fr)
Inventor
Carol A. Rouillard
Original Assignee
Unilever, N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/508,124 external-priority patent/US5571446A/en
Application filed by Unilever, N.V. filed Critical Unilever, N.V.
Priority to BR9610043A priority Critical patent/BR9610043A/pt
Priority to JP9507667A priority patent/JPH11510204A/ja
Priority to DE69605200T priority patent/DE69605200T2/de
Priority to EP96924671A priority patent/EP0842255B1/en
Priority to CA002231460A priority patent/CA2231460C/en
Priority to AU65063/96A priority patent/AU719399B2/en
Publication of WO1997005227A1 publication Critical patent/WO1997005227A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
    • C11D17/04—Detergent materials or soaps characterised by their shape or physical properties combined with or containing other objects
    • C11D17/041—Compositions releasably affixed on a substrate or incorporated into a dispensing means
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
    • C11D7/02—Inorganic compounds
    • C11D7/04—Water-soluble compounds
    • C11D7/06—Hydroxides
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/14—Hard surfaces
    • C11D2111/20—Industrial or commercial equipment, e.g. reactors, tubes or engines

Definitions

  • This invention relates to an enzyme-based cleaning system for use in clean-in-place operations to remove protein based soils.
  • Proteolytic enzymes have been used extensively in alkaline detergent formulations to aid in the removal of protein-based stains which tend to adhere to textile surfaces.
  • the most common type of formulation, which employs enzymes of this nature, are solid based detergents.
  • the enzyme in its solid stable form is mixed with alkaline solid detergent formulations containing the usual surfactants, anti-redeposition agents, water hardness control agents, other chelators and the like.
  • Solid enzymes in this type of formulation have very reliable stability over extended periods. Hence, the solid enzyme based detergent products can be packaged and stored for extended periods before use.
  • an enzyme based alkaline detergent is preferably in liquid form.
  • Such liquid forms of detergents are more readily diluted and dispersed in the cleaning formulations. They are particularly useful in cleaning of textiles because they may be applied in concentrated liquid form before the normal cleaning process.
  • Considerable effort and interest has been pursued in formulating enzyme based cleaning systems which are in a liquid form.
  • enzyme activity in liquid based detergents It is well known that cationic and the most common anionic surfactants attack enzymes, breaking them down and rendering them non-active. It is generally understood, however, that nonionic surfactants can be used in conjunction with enzymes and not appreciably affect the activity of the enzyme in a liquid formulation.
  • the presence of water in a liquid enzyme formulation causes degradation of the enzymes by self-digestion which is commonly referred to as autolysis.
  • the presence of oxygen in the liquid formulation can also present a significant problem because oxygen can denature the enzymes.
  • the presence of oxygen is normally controlled by the use of antioxidants.
  • the introduction of antioxidants to the composition can over time cause the pH of the composition to drop well below the normal alkaline pH range in which the enzymes are active. By virtue of the pH dropping, the enzymes become inactive.
  • Enzyme detergent formulations have also become useful in clean-in- place operations where it is desired to remove protein-based deposits on various types of processing equipment such as dairy equipment. Quite often in dairy processing, high temperatures are used which results in the deposit of difficult to remove soils on internal surfaces of processing equipment. Removal is normally accomplished by the use of highly alkaline or highly acidic compositions. Such compositions, although successful in removing deposited materials, are somewhat hazardous to use and must be neutralized before being discarded. Furthermore, the highly alkaline or acidic cleaning compositions are very corrosive and can attack components of the processing equipment. Alternatives have therefore been sought.
  • United States patent 4,212,761 describes a composition which is useful in cleaning processing equipment in dairy production.
  • the enzyme is particularly useful in dissolving milkstone deposits and other dairy deposits on interior surfaces of the processing equipment.
  • the composition is very useful for a clean-in-place process; however, the composition is supplied in solid form and dissolved on site in water before use.
  • Such solid composition consists essentially of a nonionic or anionic detergent, sodium carbonate or sodium bicarbonate and an alkaline protease.
  • the enzyme is stable even in the presence of the anionic detergent material.
  • the nonionic or anionic detergent material is employed solely to act as a detergent to facilitate the cleaning action where it is thought that any suitable nonionic or anionic detergent material may be used.
  • the preferred form of enzyme is a proteolytic enzyme which is capable of breaking down the deposited milk solids, particularly in the form of milkstone. Having to make up the composition on site significantly complicates the administration of the cleaning composition in a clean-in-place operation. Liquid formulations are far superior in this regard since they may be stored in drums and automatically dispensed as needed during the clean-in-place operation. United States patents 4,243,543 and 5,064,561 recognize the advantages of liquid compositions for clean-in-place systems and describe two-part compositions which are kept separate until they are combined and diluted for use in the clean-in-place operation. United States patent 4,243,543 recognizes the significant problem in stabilizing enzymes in an aqueous system.
  • an antioxidant is used to enhance stability of the enzyme in the aqueous system.
  • the enzyme-containing part of the composition comprises the proteolytic enzyme, an anionic and/or nonionic surfactant and the antioxidant with the balance being water. Because of the use of the antioxidant, the aqueous solution is not pH stable. The antioxidant will cause the pH of the solution to drop, thereby rendering the enzyme inactive over time.
  • a buffering amount of a weak base is included to stabilize pH.
  • the buffer may be any of the well-" known compositions capable of stabilizing pH, such as carbonates which have a pKa within the range of about 6 to 12.
  • a water soluble polyol containing from 2 to 6 hydroxyl groups and having a molecular weight of less than 500 is used to achieve a stable composition for storage.
  • the second component for this two-part cleaning system comprises a chelant or sequestering agent for sequestering the alkaline earth metal cations in the plant water used to dilute the two parts when combined during the clean-in-place operation.
  • United States patent 5,064,561 discloses a two-part clean-in-place system which provides for stability of the enzyme in the second concentrated solution by ensuring that the concentrate is substantially absent of free water and the enzyme is combined with a carrier such as alcohols, surfactants, polyols, glycols and mixtures thereof.
  • the first concentrate comprises a hydroxide- based alkaline material, a defoamer, a solubilizer or emulsifier and a water hardness control additive.
  • the defoamer is used to control foaming as caused by the presence of the protease in the second concentrate. It is suggested, however, that the defoamer is optional if a liquid form of the enzyme is used in the second concentrated solution.
  • the second concentrate still requires that the liquid form of the enzyme be absent of any free water as it would apply to both the source of enzyme and carrier.
  • composition in accordance with this invention provides two concentrates containing a minimum of components which surprisingly provide very effective cleaning for clean-in-place operation.
  • the second concentrate now includes water while maintaimng acceptable enzyme activity.
  • a two-part enzyme based cleaning system comprises the first and second liquid concentrates stored in separate containers where the concentrates are used in preparing a dilute use solution.
  • the first concentrate consists of: i) 1.75 to 7.5 percent by weight of a source of alkalinity selected from sources of hydroxide-based alkaline compositions; ii) 1 to 16 percent by weight of a water conditioner selected from the group consisting of polyacrylic acids and polyphosphates; and iii) balance water;
  • the second concentrate consists of: i) 5 to 45 percent by weight of an enzyme stabilizing blend of an alkali salt of a (C 6 - C 12 ) fatty acid and a linear (C 8 - C 18 ) polyoxyaikylene alcohol; ii) an effective amount of a proteolytic enzyme; iii) optionally, an enzyme compatible non-aqueous liquid polyol filler; and iv) balance water.
  • the two-part clean-in-place composition is particularly useful in cleaning of dairy processing equipment particularly as a composition used in a clean-in-place system
  • the composition may also be used in other cleaning operations where enzyme activity is desired, such as in laundry formulations, surface cleaning formulations and the like.
  • surface cleaning include removal from process equipment surfaces of brewing wort, food soils from processed foods, beverage soils (e.g., fruit juices, orange juice, juice drinks and beverages), blood in meat plants, milk based soils commonly found in the dairy industry, such as ice cream, milk, flavored milk, cream, buttermilk and the like and pharmaceutical products.
  • the composition contains a minimum of components, yet surprisingly achieves cleaning activity comparable to the far more complex multi-additive systems of the prior art, such as described in United States patents 4,243,543 and 5,064,561 and other forms of highly alkaline cleaners. It is understood that other components for purposes other than achieving enzymatic attack of proteinaceous materials may be added to the composition.
  • the essential aspect of the invention resides in the provision of a first concentrate which contains only two active ingredients and a second concentrate which contains only two different active ingredients.
  • the active ingredients are solubilized in water to provide the desired liquid concentrates.
  • the selected components of the blend which forms the first ingredient of the second concentrate stabilizes the enzyme and ensures its activity when used with the first concentrate to provide a diluted solution effective for use in a clean-in-place operation.
  • enzyme compatible non-aqueous fillers may be used.
  • enzyme activity can vary greatly based on the "source of the enzyme either extracted from natural sources or isolated from a culture of bacteria which under appropriate conditions manufacture the enzyme.
  • the main objective is to provide in the second concentrate sufficient active enzyme which, when provided in the use dilution, is able to digest the proteinaceous soils and provide the desired cleaning action.
  • a sufficient amount is incorporated in the concentrate to provide the desired cleaning.
  • the second concentrate, which contains the enzyme is sufficiently stabilized by the blend of ingredients that after considerable storage time, sufficient activity remains to effect the desired cleaning. For example, it has been found that storage of the second concentrate at normal storage temperatures for up to three months does not greatly affect the enzyme activity.
  • the first and second concentrates provide a cleaning system which includes fewer ingredients and is therefore more cost effective for use in cleaning operations.
  • the blend of active ingredients which provides for the stability of the proteolytic enzyme is a combination of an alkali salt of a (C 6 - C 12 ) fatty acid and a linear (C 8 - C 18 ) polyoxyalkylenealcohols.
  • the fatty acid is preferably C 8 - C 10 such as octanoic acid, nonanoic acid and decanoic acid.
  • the preferred alkali salts thereof are potassium and sodium.
  • the linear polyoxyaikylene is considered to be a nonionic with C 8 to C 18 carbon atoms in the linear alkyl chain, where the chain terminating in alcohol, is usually either ethoxylated and/or propoxylated.
  • the components of the blend can be readily obtained from a host of suppliers of amonic and nonionic surfactants. This blend of components has been found to be compatible with the selected proteolytic enzyme, such that when stored in water is not attacked by the blend and prevents autolysis in the water so that the enzyme activity is maintained during a normal shelf life expectancy period.
  • the preferred enzyme is an endoproteinase of the serine type.
  • the effective amount of the enzyme in the concentrate is sufficient to provide the desired degree of activity which is usually in excess of 85% of the original activity as previously described.
  • the preferred enzyme is sold under the trademark ESPERASE and may be obtained from Novo Industries of Denmark.
  • the enzyme is prepared by submerged fermentation of a selected microorganism that can be classified as an alkalophilic species of Bacillus. This type of enzyme has a very broad substrate specificity and is capable of hydrolyzing most peptide bonds within a protein molecule.
  • the first concentrate consists of 1.75% to 7.5% by weight of the concentrate of an hydroxide-based alkaline composition.
  • Preferred hydroxides for the alkaline composition are potassium and/or sodium hydroxide.
  • the alkaline composition preferably includes just the hydroxide, but in some use situations, may include other alkalinity enhancers. Although in keeping with a preferred aspect of the invention, additives and enhancers can " be avoided.
  • the water conditioner is preferably from 1 to 16% by weight of the concentrate.
  • the water conditioner is selected from the group of polyphosphates and polyacrylic acids.
  • the polyacrylic acids act as anti ⁇ redeposition agents and have a molecular weight ranging from 3000 to 6000 where the preferred polyacrylate is a homopolymer sold under the trademark ACUSOL 445 by Rohm and Haas Company.
  • Other polyacrylates include copolymers of acrylic acid, maleic acid and other olefins and terpolymers which are a mixture of monomers.
  • the polyacrylate is normally available in a solution, where the solution is 48% polyacrylic acid and the balance water.
  • Other forms of water conditioners include various polyphosphates, such as sodium tripolyphosphate and potassium tripolyphosphate.
  • the order of addition of the concentrates to water for end use is, as would be expected, conducted in a manner to protect the activity of the enzyme. Since the first concentrate has a high pH, it would, as one skilled in the art appreciates, be detrimental to the enzyme activity to combine it directly with the first concentrate before dilution. The high pH in the first concentrate would greatly reduce the activity of the enzyme. Alternatively if the "second concentrate were first diluted and then the first concentrate added to the diluted second concentrate, there is also the possibility of reducing enzyme activity, because the introduced first concentrate of high pH may in localized regions of the diluted second concentrate attack the enzyme and reduce its activity.
  • the preferred order of diluting the concentrates is as follows. The first concentrate is diluted to the desired use solution range.
  • the second concentrate is added to the diluted first concentrate to minimize any possibility of affecting enzyme activity.
  • the rate of addition of the second concentrate to the diluted first concentrate can vary depending upon the manner in which the use solution is formulated; that is either by injection or mixing in a stir tank.
  • Preferred amounts in the first concentrate of the alkaline material is approximately 2.5% and of the polyacrylic acid of about 4%.
  • the preferred amount of the enzyme stabilizing blend is approximately 40% by weight and sufficient enzyme to provide the desired activity level.
  • the use solution provided by diluting the first and second concentrates with water provides from about 0.02 to 1 % by weight of the total weight of the use solution of the first concentrate, and about 0.0002 to 0.05% by weight (2 ppm to 500 ppm) of the second concentrate.
  • the preferred weight range for the first concentrate in the use solution is about 0.04 to 0.6% by weight of solution and about 0.005% to 0.11% by weight (50 ppm to 1100 ppm) of the second concentrate.
  • the use solution is circulated through the equipment in the normal clean-in-place process.
  • the preferred temperature for the use solution to effect optimum enzyme activity is in the range of 50°C to 60°C where the pH is preferably in the range of 8.5 to 10.5.
  • the use solution is considerably less alkaline than the previous use solutions, particularly the highly alkaline cleaning solutions.
  • the second concentrate provides a stable enzyme composition under normal storage conditions.
  • the amount of water in the second concentrate may be well in excess of 30% and may be as high as approximately 65% by weight of water.
  • Such high water levels in the second concentrate permits the use of off-the-shelf supplies for both the blend of anionic detergent and liquid forms of the enzyme.
  • the enzyme composition as commercially obtained from Novo for example, may have considerable quantities of water.
  • a further improvement in this composition is in respect of providing a more dilute second concentrate to facilitate more accurate dispensing of the enzyme into the use solution.
  • the enzyme is stabilized by the blend of the alkali salt of a fatty acid and the linear polyoxyaikylene alcohols. With the enzyme stabilized, the amount of water in the concentrate could be increased to further dilute the concentration of the enzyme in the second concentrate.
  • suitable non-aqueous fillers which are compatible with the enzyme, maintains phase stability and optionally provides anti-freeze properties.
  • non-aqueous fillers are polyols, preferably with 2 to 6 carbon atoms.
  • suitable non-aqueous fillers include propylene glycol, 1,2-propane-diol, butylene glycol, ethylene glycol, hexeleneglycol, erythritol, fructose, glucose, glycerol, lactose, mannitol and sorbitol.
  • the use of the non ⁇ aqueous filler maintains an acceptable ratio of enzyme to water while at the same time providing a more dilute concentration of the enzyme in the second concentrate to facilitate more accurate dosing by dispensing at each opportunity a greater volume of the second concentrate.
  • the non ⁇ aqueous filler By using the non ⁇ aqueous filler, not only is the ratio of enzyme to stabilizing blend and ratio of enzyme to water kept in line, but as well the ratio of the stabilizing blend to water is also kept in range to ensure shelf-life of the more dilute enzyme in the concentrate and reduce the risks of freezing and phase instability.
  • the cleaner according to this invention, has many significant business advantages. From a production standpoint, a very trim formulation in the sense of very few ingredients is provided so that manufacture of the cleaner is greatly facilitated. There is no requirement to add various additives to maintain enzyme stability, other than the unique formulation provided in the form of a single blend which is combined with the enzyme. Production is greatly facilitated in that water is now accommodated in the formulation.
  • the system when cleaning, functions at a considerably reduced pH compared to the well known chlorinated alkaline cleaners. Hence less treatment is required to discharge the cleaning effluent.
  • the cleaner in accordance with this invention, may be used to clean a variety of other pieces of food handling equipment which contain a variety of other forms of proteinaceous soils; for example, juice dispensing systems, ice cream manufacturing equipment, fast food preparation equipment, brewery fermentation and liquid handling equipment, and even equipment which handles high fat, low protein food materials such as cream handling equipment. It is quite surprising that the formulation of this invention is successful in cleaning tank, processing equipment and the like which handles cream. Cream is very high in fat, usually 40% or more but has a very low protein content.
  • a chlorinated alkali material or solvent is required.
  • these cleaners require considerable processing and treatment before release to the environment.
  • the cleaner of this formulation which is low in pH and does not include a solvent works quite effectively in removing cream residues from surfaces of the handling equipment.
  • the cleaning formulation of this invention is commercially quite usable in that a single cleaner can be used for a variety of cleaning tasks in a food processing facility. This greatly reduces costs in overall cleaning management of the food processing equipment as well as providing much greater safety in the handling of the cleaning composition, certainly compared to the far more hazardous cleaners, such as chlorinated alkali.
  • Exemplary compositions for the first and second concentrates are provided in the following Tables 1 and 2. The various concentrates as diluted were used in accordance with the following tests to give the results provided in Table 3.
  • Alkali surfactant 8 0.0 0.0 1.0 1.0 1.0
  • Typical use concentration of Component 2 is 0.005 to
  • the panels were suspended from a rod and hook assembly and were soiled by completely immersing them in homogenized, pasteurized whole milk held at 8° to 12°C for 10 minutes. They were then removed from the milk, rinsed and immediately suspended for a period of 10 minutes in test solution held at 60°C. After cleaning, diey were thoroughly rinsed with cold tap water, followed by a deionized water rinse. The sheeting action of the water was noted at this point. This procedure was repeated 9 more times, for a total of 10 cleaning cycles. A final evaluation was done by soaking the panels in a solution of dye that stains organic soils red. Panels that exhibited complete water sheeting after every cycle and did not retain any red dye were deemed efficacious. A chlorinated alkaline cleaner "INTEREST", available from Diversey Inc., was used as the positive control.
  • Table 3 shows some test results, indicating the combination of components used; test temperature, water hardness level and result.
  • a representative composition for the second concentrate is set out in the following Table 5.
  • the actual amount of water in the composition is 47.5% by weight and the active amount in the anionic blend is 10% by weight.
  • the two-part enzyme-based cleaning system comprising the first and second liquid concentrates may have a range in respect of percent by weight of the active components of each concentrate. Such ranges are exemplified by the above examples.
  • the preferred percent by weight of the source of alkalinity is about 2 to 4%.
  • the preferred percent by weight of the water conditioner is about 4 to 6%.
  • the preferred weight of the enzyme stabilizing blend for the more concentrated solution is 35 to 45 % by weight, whereas when it is desired to have a more diluted concentration of the enzyme, the blend may be in the range of 10 to 20% by weight.
  • the percent by weight of the non-aqueous liquid filler, when more dilute concentrations of the enzyme are desired is normally in the range of 25 to 55% by weight.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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PCT/US1996/012052 1995-07-27 1996-07-22 An anionic stabilized enzyme-based clean-in-place system WO1997005227A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
BR9610043A BR9610043A (pt) 1995-07-27 1996-07-22 Sistema de limpeza de duas partes à base de enzimas
JP9507667A JPH11510204A (ja) 1995-07-27 1996-07-22 アニオン安定化酵素現場洗浄系
DE69605200T DE69605200T2 (de) 1995-07-27 1996-07-22 Cip (cleaning-in-place) system auf basis von mit einem anionischen tensid stabilisiertem enzym
EP96924671A EP0842255B1 (en) 1995-07-27 1996-07-22 An anionic stabilized enzyme-based clean-in-place system
CA002231460A CA2231460C (en) 1995-07-27 1996-07-22 An anionic stabilized enzyme-based clean-in-place system
AU65063/96A AU719399B2 (en) 1995-07-27 1996-07-22 An anionic stabilized enzyme-based clean-in-place system

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/508,124 US5571446A (en) 1995-07-27 1995-07-27 Anionic stabilized enzyme based clean-in-place system
US08/660,530 US5783542A (en) 1995-07-27 1996-06-07 Anionic stabilized enzyme based clean-in-place system
US08/660,530 1996-06-07
US08/508,124 1996-06-07

Publications (1)

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WO1997005227A1 true WO1997005227A1 (en) 1997-02-13

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PCT/US1996/012052 WO1997005227A1 (en) 1995-07-27 1996-07-22 An anionic stabilized enzyme-based clean-in-place system

Country Status (9)

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EP (1) EP0842255B1 (pt)
JP (1) JPH11510204A (pt)
AR (1) AR003018A1 (pt)
AU (1) AU719399B2 (pt)
BR (1) BR9610043A (pt)
CA (1) CA2231460C (pt)
DE (1) DE69605200T2 (pt)
ES (1) ES2140111T3 (pt)
WO (1) WO1997005227A1 (pt)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999005252A1 (de) * 1997-07-22 1999-02-04 Henkel-Ecolab Gmbh & Co. Ohg Verwendung enzymhaltiger lösungen zum reinigen von gär- oder lagertanks
WO2004053042A1 (de) 2002-12-06 2004-06-24 Henkel Kommanditgesellschaft Auf Aktien Mehrkomponenten-flüssigwaschmittel
US7494963B2 (en) 2004-08-11 2009-02-24 Delaval Holding Ab Non-chlorinated concentrated all-in-one acid detergent and method for using the same
US7569532B2 (en) 2000-06-29 2009-08-04 Ecolab Inc. Stable liquid enzyme compositions
US7786063B2 (en) 2003-07-14 2010-08-31 Kao Corporation Detergent composition for CIP comprising a C10-C14 aliphatic hydrocarbon and nonionic surfactant
US7795199B2 (en) 2000-06-29 2010-09-14 Ecolab Inc. Stable antimicrobial compositions including spore, bacteria, fungi, and/or enzyme
US7803604B2 (en) 2000-07-28 2010-09-28 Henkel Ag & Co. Kgaa Amylolytic enzyme extracted from Bacillus sp. A 7-7 (DSM 12368) and washing and cleaning agents containing this novel amylolytic enzyme
US7888104B2 (en) 2000-11-28 2011-02-15 Henkel Ag & Co. Kgaa Cyclodextrin glucanotransferase (CGTase), obtained from<I>Bacillus agaradherens<λ>(DSM 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase

Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
JP2009019130A (ja) * 2007-07-12 2009-01-29 Adeka Corp 洗浄剤組成物及びそれを使用した食器類の洗浄方法
US10010090B2 (en) 2012-05-23 2018-07-03 Normand Lauzon Method for controlling microbiological contamination in a heat exchanger while processing a food product

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Publication number Priority date Publication date Assignee Title
US4243543A (en) * 1979-05-11 1981-01-06 Economics Laboratory, Inc. Stabilized liquid enzyme-containing detergent compositions
EP0104434A2 (de) * 1982-09-02 1984-04-04 Henkel Kommanditgesellschaft auf Aktien Flüssiges, von anorganischen Gerüstsalzen im wesentlichen freies Wasch- und Reinigungsmittel
US5064561A (en) * 1990-05-09 1991-11-12 Diversey Corporation Two-part clean-in-place system
WO1996006910A2 (en) * 1994-08-31 1996-03-07 Ecolab Inc. Improved proteolytic enzyme cleaner

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4243543A (en) * 1979-05-11 1981-01-06 Economics Laboratory, Inc. Stabilized liquid enzyme-containing detergent compositions
EP0104434A2 (de) * 1982-09-02 1984-04-04 Henkel Kommanditgesellschaft auf Aktien Flüssiges, von anorganischen Gerüstsalzen im wesentlichen freies Wasch- und Reinigungsmittel
US5064561A (en) * 1990-05-09 1991-11-12 Diversey Corporation Two-part clean-in-place system
WO1996006910A2 (en) * 1994-08-31 1996-03-07 Ecolab Inc. Improved proteolytic enzyme cleaner

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999005252A1 (de) * 1997-07-22 1999-02-04 Henkel-Ecolab Gmbh & Co. Ohg Verwendung enzymhaltiger lösungen zum reinigen von gär- oder lagertanks
US6564813B1 (en) 1997-07-22 2003-05-20 Ecolab Gmbh & Co. Ohg Use of solutions containing enzymes for cleaning fermentation or storage tanks
US6799585B2 (en) 1997-07-22 2004-10-05 Ecolab Gmbh Co. Ohg Use of solutions containing enzymes for cleaning fermentation or storage tanks
US7569532B2 (en) 2000-06-29 2009-08-04 Ecolab Inc. Stable liquid enzyme compositions
US7795199B2 (en) 2000-06-29 2010-09-14 Ecolab Inc. Stable antimicrobial compositions including spore, bacteria, fungi, and/or enzyme
US7803604B2 (en) 2000-07-28 2010-09-28 Henkel Ag & Co. Kgaa Amylolytic enzyme extracted from Bacillus sp. A 7-7 (DSM 12368) and washing and cleaning agents containing this novel amylolytic enzyme
US7888104B2 (en) 2000-11-28 2011-02-15 Henkel Ag & Co. Kgaa Cyclodextrin glucanotransferase (CGTase), obtained from<I>Bacillus agaradherens<λ>(DSM 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase
WO2004053042A1 (de) 2002-12-06 2004-06-24 Henkel Kommanditgesellschaft Auf Aktien Mehrkomponenten-flüssigwaschmittel
US7786063B2 (en) 2003-07-14 2010-08-31 Kao Corporation Detergent composition for CIP comprising a C10-C14 aliphatic hydrocarbon and nonionic surfactant
US7494963B2 (en) 2004-08-11 2009-02-24 Delaval Holding Ab Non-chlorinated concentrated all-in-one acid detergent and method for using the same
US7501027B2 (en) 2004-08-11 2009-03-10 Delaval Holding Ab Non-chlorinated concentrated all-in-one acid detergent and method for using the same

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AU6506396A (en) 1997-02-26
DE69605200D1 (de) 1999-12-23
BR9610043A (pt) 1999-07-06
AR003018A1 (es) 1998-05-27
EP0842255B1 (en) 1999-11-17
CA2231460C (en) 2005-02-01
AU719399B2 (en) 2000-05-11
EP0842255A1 (en) 1998-05-20
CA2231460A1 (en) 1997-02-13
DE69605200T2 (de) 2000-04-06
ES2140111T3 (es) 2000-02-16
JPH11510204A (ja) 1999-09-07

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