WO1996040242A1 - Vaccine comprising a polysaccharide antigen-carrier protein conjugate and free carrier protein - Google Patents

Vaccine comprising a polysaccharide antigen-carrier protein conjugate and free carrier protein Download PDF

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Publication number
WO1996040242A1
WO1996040242A1 PCT/EP1996/002436 EP9602436W WO9640242A1 WO 1996040242 A1 WO1996040242 A1 WO 1996040242A1 EP 9602436 W EP9602436 W EP 9602436W WO 9640242 A1 WO9640242 A1 WO 9640242A1
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Prior art keywords
vaccine
carrier protein
polysaccharide
antigen
combined vaccine
Prior art date
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Ceased
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PCT/EP1996/002436
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English (en)
French (fr)
Inventor
Moncef Mohamed Slaoui
Pierre Hauser
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GlaxoSmithKline Biologicals SA
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SmithKline Beecham Biologicals SA
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Priority claimed from GBGB9512827.8A external-priority patent/GB9512827D0/en
Priority claimed from GBGB9513443.3A external-priority patent/GB9513443D0/en
Priority claimed from GBGB9525657.4A external-priority patent/GB9525657D0/en
Priority to AU62221/96A priority Critical patent/AU695720B2/en
Priority to CA2221511A priority patent/CA2221511C/en
Priority to BR9608612A priority patent/BR9608612A/pt
Priority to DE69615362T priority patent/DE69615362T3/de
Priority to AT96920790T priority patent/ATE205724T1/de
Priority to JP50014697A priority patent/JP4233113B2/ja
Priority to EP96920790A priority patent/EP0831901B2/en
Priority to NZ311000A priority patent/NZ311000A/en
Priority to PL96323863A priority patent/PL323863A1/xx
Application filed by SmithKline Beecham Biologicals SA filed Critical SmithKline Beecham Biologicals SA
Publication of WO1996040242A1 publication Critical patent/WO1996040242A1/en
Priority to NO975555A priority patent/NO975555L/no
Anticipated expiration legal-status Critical
Priority to US10/949,123 priority patent/US20050129707A1/en
Priority to US11/613,797 priority patent/US20070116714A1/en
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/09—Lactobacillales, e.g. aerococcus, enterococcus, lactobacillus, lactococcus, streptococcus
    • A61K39/092—Streptococcus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0016—Combination vaccines based on diphtheria-tetanus-pertussis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/05—Actinobacteria, e.g. Actinomyces, Streptomyces, Nocardia, Bifidobacterium, Gardnerella, Corynebacterium; Propionibacterium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/08—Clostridium, e.g. Clostridium tetani
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/095—Neisseria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/102—Pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/125—Picornaviridae, e.g. calicivirus
    • A61K39/13—Poliovirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/29—Hepatitis virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55505—Inorganic adjuvants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
    • A61K2039/6031—Proteins
    • A61K2039/6037—Bacterial toxins, e.g. diphteria toxoid [DT], tetanus toxoid [TT]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/70—Multivalent vaccine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32611—Poliovirus
    • C12N2770/32634—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the present invention relates to combination vaccines comprising a conjugated polysaccharide antigen linked to a carrier protein, and wherein the 5 carrier protein is also present as a free antigen in the vaccine composition.
  • the vaccine composition of the present invention relates to a multivalent vaccine, that is a vaccine for the amelioration or treatment of more than one disease states.
  • the present invention also relates to the production and use of such a vaccine in medicine.
  • Vaccines that utilise polysaccharides are known in the art. For example a vaccine for the prevention of Haemophilus influenzae b (Hib) infections are based on the capsular polysaccharide (PRP) conjugated with a carrier protein.
  • PRP capsular polysaccharide
  • the carrier protein is a diphtheria or tetanus toxoid. It has also been suggested to produce a vaccine for the prevention of Streptococcus pneumonia which is based on 5 capsular polysaccharide conjugated to a carrier protein such as tetanus toxoid or diphtheria toxoid. Examples of such conjugate vaccine antigens are disclosed in US 4 365 170 US 4 673 574 EP 208 375 EP 477508 and EP 161 188.
  • One well known combination vaccine provides protection against Diphtheria , tetanus and B. 5 pertussis infections.
  • This vaccine comprises a pertussis component (either killed whole cell B. pertussis or accellular pertussis which typically consists of up to three antigens - PT, FHA and 69kDa) and toxoided diphtheria and tetanus toxin.
  • Such vaccines are often referred to as DTPw (whole cell) or DTPa (accellular).
  • a vaccine comprises just a single polysaccharide (PS) conjugated to a carrier protein
  • PS polysaccharide
  • the protein carrier needs to be present at concentrations (ie PS to protein ratio) such that a good TH cell response to the carrier is induced, and that this TH response can recognise PS specific B cell clones expressing peptides derived from the covalently coupled carrier protein.
  • the prior art monovalent vaccines have a ratio of polysaccharide: protein carrier of 1 :3 (weight:weight).
  • the present inventors have discovered that when the polysaccharide conjugate component is part of a multivalent vaccine comprising a free form of the carrier protein, the immunogenicity of the polysaccharide component is improved by reducing the protein content of the conjugate antigen.
  • the present invention provides a combined vaccine containing a polysaccharide antigen conjugated to a carrier protein and wherein the carrier protein is also present as free antigen characterised in that the ratio of polysaccharide to carrier protein is from approximately 1:0.3 to 1:2 .
  • the polysaccharide conjugate may be prepared by any known coupling technique.
  • the polysaccharide can be coupled via a thioether linkage.
  • This conjugation method relies on activation of the polysaccharide with l-cyano-4- dimethylamino pyridinium tetrafluoroborate (CDAP) to form a cyanate ester.
  • CDAP l-cyano-4- dimethylamino pyridinium tetrafluoroborate
  • the activated polysaccharide may thus be coupled directly or via a spacer group to an amino group on the carrier protein.
  • the cyanate ester is coupled with hexane diamine and the amino-derivatised polysaccharide is conjugated to the carrier protein using heteroligation chemistry involving the formation of the thioether linkage.
  • heteroligation chemistry involving the formation of the thioether linkage.
  • the conjugates can also be prepared by direct reductive amination methods as described in US 4365170 (Jennings) and US 4673574 (Anderson). Other methods are described in EP-0-161-188, EP-208375 and EP-0-477508.
  • a further method involves the coupling of a cyanogen bromide activated polysaccharide derivatised with adipic acid hydrazide (ADH) to the protein carrier by Carbodiimide condensation (Chu C. et al Infect. Immunity, 1983 245 256).
  • the carrier protein is an antigen derived from a Tetanus, or Diphtheria preferably tetanus toxoid or Diphtheria toxoid.
  • the carrier may also be derived from Bordetella.
  • the antigen is non toxic derivative of a toxin from Tetanus or Diphtheria.
  • compositions of the invention contain a suitable adjuvant.
  • MPL (3-deacylated monophosphoryl lipid A, also known as 3D-MPL).
  • 3D-MPL is known from GB 2 220 211 (Ribi) as a mixture of 3 De-O-acylated monophosphoryl lipid A with 4, 5 or 6 acylated chains and is manufactured by Ribi Immunochem, Montana.
  • Ribi Immunochem Montana.
  • a preferred form of MPL is disclosed in International Patent Application 92/116556.
  • QS21 Another adjuvant which may be used in the present invention is known as QS21.
  • QS21 is a Hplc purified non toxic fraction of a saponin from the bark of the South American tree Quillaja saponaria molina and its method of its production is disclosed (as QA21) in US patent No. 5,057,540.
  • Combinations of QS21 and 3 D-MPL are known to produce a synergistically effective vaccine formulation and are described in International Patent application WO 94/00153.
  • Hib component vaccines of the invention will not require any specific carrier and be formulated in an aqueous or other pharmaceutically acceptable buffer. In some cases it may be that the entire vaccine of the present invention will be presented in an oil in water emulsion, or other suitable vehicle, such as for example, liposomes, microspheres or encapsulated antigen particles.
  • the vaccine formulations may contain further antigens.
  • Antigen or antigenic compositions known in the art can be used in the compositions of the invention, including antigen or antigenic compositions derived from HIV-1, (such as gpl20 or gpl60), human or animal herpes viruses, such as gD or derivatives thereof or Immediate Early protein such as ICP27 from HSV1 or HSV2, cytomegalovirus (especially human)(such as gB or derivatives thereof), Varicella Zoster Virus (such as gpl, II or III), or from a hepatitis virus such as hepatitis B virus for example Hepatitis B Surface antigen or a derivative thereof, hepatitis A virus, hepatitis C virus and hepatitis E virus, or from other viral pathogens, such as Respiratory Syncytial virus, human papilloma virus or Influenza virus, or polio virus such as Inactivated Polio Virus (IPV) or derived from
  • Borrelia for example OspA or OspB or derivatives thereof
  • Chlamydia for example P.69
  • PT and FHA from B. pertussis (P)
  • derived from parasites such as plasmodium or Toxoplasma.
  • Particularly preferred vaccines of the invention include, DTPa Hib; and DTPa Hib Hep B; and DTPa Hib Hep B IPV.
  • the Hib component may be formulated with the other antigens just prior to administration.
  • a lyopholised Hib component may be reconstituted prior to use, by mixing with the aqueous formulation of other antigens.
  • the above combinations may optionally include a component which is protective against Hepatitis A.
  • the IPV component may be the Salk inactivated polio vaccine.
  • the Diphtheria, Tetanus and Pertussis vaccine may comprise an accellular product such as Infanrix DTPa (SmithKline Beecham Biologicals) .
  • the component affording protection against Hepatitis A is preferably the product known as 'Havrix' (SmithKline Beecham Biologicals) which is a killed attenuated vaccine derived from the HM-175 strain of HAV [see 'Inactivated Candidate Vaccines for Hepatitis A' by F.E.
  • the Hepatitis B component may comprise the 'S' antigen as in 'Engerix-B'.
  • Haemophilus Influenzae B vaccine or combination vaccine according to the invention is a paediatric vaccine.
  • Vaccine preparation is generally described in Vaccine Design - the subunit and Adjuvant approach edited by Michael F Powell and Mark Newman, Plenum Press. Encapsulation within liposomes is described, for example, by Fullerton, US Patent 4,235,877. Conjugation of proteins to macromolecules is disclosed, for example, by Likhite, US Patent 4,372,945 and by Armor et al., US Patent 4,474,757.
  • the amount of antigen in each vaccine dose is selected as an amount which induces an immunoprotective response without significant, adverse side effects in typical vaccinees. Such amount will vary depending on which specific immunogens are employed. Generally it is expected that each dose will comprise l-1000ug of total immunogen, preferably 2-100ug, most preferably 4-40ug. An optimal amount for a particular vaccine can be ascertained by standard studies involving observation of antibody titres and other responses in subjects. Following an initial vaccination, subjects may receive one or two booster injections at about 4 weeks intervals. The following examples will illustrate the invention. Examples
  • Example la Vaccine comprising DTPa - Hib PRP-TT - and HBsAg.
  • Hib PRP is extracted and purified from inactivated cell cultures.
  • the purified material meets the WHO and US specifications in terms of residual protein, nucleic acid, endotoxin, structural sugars and molecular size distribution.
  • Tetanus toxoid produced by Behringwerke meets also the WHO specifications.
  • the material is further purified by acid pH precipitation and gel filtration chromatography to isolate the monomeric form (150 kDa) of TT.
  • Hib PRP 20 mg were dissolved in 4 ml of 2 M Na Cl.
  • 150 mcl of CDAP l-cyano-4-dime ylamino-pyridinium tetrafluoroborate
  • 300 mcl of triemylamine 0.2 M was added. The activation of the polysaccharide was performed during 2 minutes at 0°C at pH 9.5- 10.
  • the conjugate is purified by gel filtration chromatography using a Sephacryl HR 500 gel filtration column equilibrated with 0.2 M NaCl. The carbohydrate and protein content of the eluted fractions was determined. The conjugate was pooled in and sterile filtered. The PRP/protein ratio and free PS content in the conjugate preparation were determined.
  • DTPa comprising Diphtheria toxoid, Tetanus toxoid, pertussis toxoid, pertactin and FHA
  • DTPa Comprising Diphtheria toxoid, Tetanus toxoid, pertussis toxoid, pertactin and FHA
  • a vaccine is disclosed in International Patent application No WO 93/24148.
  • the Conjugate as described in example 1 was added to the vaccine and mixed prior to injection for immunogenicity studies.
  • the final composition of the vaccine was for one dose:
  • the immunogenicity of Haemophilus Influenzae type B derived polysaccharide :protein (weight: weight) ratios (1:3, 1:2 and 1: 1), using two different coupling chemistries, is compared after a first and second inoculation in young OFA rats.
  • Groups of 10 rats (OFA, 5 weeks of age, female) were immunised 2 times at 24 days interval by the subcutaneous route with the different TT-PRP vaccines given at 2 dosages: 0.5 ⁇ PS/rat (table 2) or 0.05 ⁇ PS/rat (table 3).
  • the HibOOl vaccine was reconstituted with saline or 1 dose of DTPa (lot 119) to have a concentration of lO ⁇ PS/ml.
  • the liquid vaccines were diluted with saline or 1 human dose of DTPa to have lO ⁇ PS/ml.
  • the vaccines were then further diluted in saline to have 2.5 ⁇ PS/ml or 0.25 ⁇ PS/ml.
  • the rats were injected with 200 ⁇ l of each vaccine within one hour after dilution.
  • Groups of 10 rats (OFA, 1 week of age) were immunised three times (SC) at two week intervals with 0.5 ⁇ g of PS combined 1 hour before injection with l/20th a human dose of DTPa or DTPa HepB.
  • the animals were bled 2 weeks after the third dose and the antibodies against pertussis toxoid (PT), FHA, pertactin (69k), Diphtheria toxoid (D) Tetanus toxoid (T) and Hepatitis B surface antigen (HBS) measured by Elisa.
  • the titres expressed as a mid point dilution using a reference are shown in Table 4.

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PCT/EP1996/002436 1995-06-07 1996-06-04 Vaccine comprising a polysaccharide antigen-carrier protein conjugate and free carrier protein Ceased WO1996040242A1 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
EP96920790A EP0831901B2 (en) 1995-06-07 1996-06-04 Vaccine comprising a polysaccharide antigen-carrier protein conjugate and free carrier protein
NZ311000A NZ311000A (en) 1995-06-07 1996-06-04 Vaccine comprising a polysaccharide antigen-carrier protein conjugate and a free carrier protein
PL96323863A PL323863A1 (en) 1995-06-23 1996-06-04 Vaccine containing a conjugate of polysaccharidic antigen with proteinous carrier and free proteinous carrier
CA2221511A CA2221511C (en) 1995-06-07 1996-06-04 Vaccine comprising a polysaccharide antigen-carrier protein conjugate and free carrier protein
AU62221/96A AU695720B2 (en) 1995-06-07 1996-06-04 Vaccine comprising a polysaccharide antigen-carrier protein conjugate and free carrier protein
BR9608612A BR9608612A (pt) 1995-06-07 1996-06-04 Vacina compreendendo um conjugado de antígeno de polissacarídeo - proteína portadora e proteína portadora livre
DE69615362T DE69615362T3 (de) 1995-06-07 1996-06-04 Impfstoff mit einem polysaccharidantigen-trägerprotein-konjugat und einem trägerprotein
AT96920790T ATE205724T1 (de) 1995-06-07 1996-06-04 Vakzine mit einem polysaccharide antigen- trägerprotein konjugat und freien trägerprotein
JP50014697A JP4233113B2 (ja) 1995-06-07 1996-06-04 多糖類抗原−キャリア蛋白接合体および遊離キャリア蛋白を有してなるワクチン
NO975555A NO975555L (no) 1995-06-07 1997-12-02 Vaksine omfattende polysakkaridangiten-proteinbærer-konjugat og fritt bærerprotein
US10/949,123 US20050129707A1 (en) 1995-06-23 2004-09-24 Vaccine comprising a polysaccharide antigen - carrier protein conjugate and free carrier protein
US11/613,797 US20070116714A1 (en) 1995-06-07 2006-12-20 Vaccine Comprising a Polysaccharide Antigen-Carrier Protein Conjugate and Free Carrier Protein

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US47263995A 1995-06-07 1995-06-07
US08/472,639 1995-06-07
GBGB9512827.8A GB9512827D0 (en) 1995-06-23 1995-06-23 Vaccines
GB9512827.8 1995-06-23
GB9513443.3 1995-07-01
GBGB9513443.3A GB9513443D0 (en) 1995-07-01 1995-07-01 Vaccines
GB9525657.4 1995-12-15
GBGB9525657.4A GB9525657D0 (en) 1995-12-15 1995-12-15 Vaccines

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US08973524 A-371-Of-International 1998-01-28
US10/383,326 Continuation US20030157129A1 (en) 1995-06-07 2003-03-07 Vaccine comprising a polysaccharide antigen - carrier protein conjugate and free carrier protein

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WO1996040242A1 true WO1996040242A1 (en) 1996-12-19

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PCT/EP1996/002436 Ceased WO1996040242A1 (en) 1995-06-07 1996-06-04 Vaccine comprising a polysaccharide antigen-carrier protein conjugate and free carrier protein

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EP (2) EP0831901B2 (enExample)
JP (2) JP4233113B2 (enExample)
AT (2) ATE400295T1 (enExample)
AU (1) AU695720B2 (enExample)
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AU695720B2 (en) 1998-08-20
PT1090642E (pt) 2008-09-09
DE69615362D1 (de) 2001-10-25
DE69615362T2 (de) 2002-06-13
CA2221511C (en) 2013-01-08
BR9608612A (pt) 1999-05-04
CZ390297A3 (cs) 1998-05-13
HUP9802199A1 (hu) 1999-01-28
EP1090642A3 (en) 2001-08-22
DE69615362T3 (de) 2006-07-20
HUP9802199A3 (en) 1999-07-28
DK1090642T3 (da) 2008-09-22
JP4233113B2 (ja) 2009-03-04
JP2007186524A (ja) 2007-07-26
CA2221511A1 (en) 1996-12-19
EP0831901B1 (en) 2001-09-19
NO975555D0 (no) 1997-12-02
ES2164251T3 (es) 2002-02-16
ATE205724T1 (de) 2001-10-15
JPH11507026A (ja) 1999-06-22
EP1090642A2 (en) 2001-04-11
EP0831901A1 (en) 1998-04-01
IL122431A0 (en) 1998-06-15
ES2164251T5 (es) 2006-06-01
TR199701547T1 (xx) 1998-03-21
EP1090642B1 (en) 2008-07-09
ES2308962T3 (es) 2008-12-16
MX9709732A (es) 1998-10-31
AU6222196A (en) 1996-12-30
ATE400295T1 (de) 2008-07-15
EP0831901B2 (en) 2005-12-07
NO975555L (no) 1998-01-09
DE69637597D1 (de) 2008-08-21
SI1090642T1 (sl) 2008-12-31
NZ311000A (en) 1999-04-29
JP4653131B2 (ja) 2011-03-16
EP1090642B8 (en) 2010-08-11

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