WO1996039536A1 - PROCEDE CHIMIQUE UNIVERSEL DE QUANTIFICATION DE L'EXPRESSION DE L'ARNm UTILISANT UN DOSAGE IMMUNOENZYMATIQUE - Google Patents

PROCEDE CHIMIQUE UNIVERSEL DE QUANTIFICATION DE L'EXPRESSION DE L'ARNm UTILISANT UN DOSAGE IMMUNOENZYMATIQUE Download PDF

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Publication number
WO1996039536A1
WO1996039536A1 PCT/US1996/008142 US9608142W WO9639536A1 WO 1996039536 A1 WO1996039536 A1 WO 1996039536A1 US 9608142 W US9608142 W US 9608142W WO 9639536 A1 WO9639536 A1 WO 9639536A1
Authority
WO
WIPO (PCT)
Prior art keywords
probe
pcr product
primer
mrna
microtiter plate
Prior art date
Application number
PCT/US1996/008142
Other languages
English (en)
Inventor
Constance Emmett
Kimberly A. Foster
Original Assignee
T Cell Diagnostics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by T Cell Diagnostics, Inc. filed Critical T Cell Diagnostics, Inc.
Priority to AU58851/96A priority Critical patent/AU5885196A/en
Publication of WO1996039536A1 publication Critical patent/WO1996039536A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Definitions

  • an assay for the detection of mRNA expression of a selected protein in a biological sample using a universal enzyme-linked immunosorbent assay (ELISA) of probed polymerase chain reaction (PCR) products resulting from reverse transcriptase-PCR (RT-PCR) of the mRNA of interest.
  • biological sample it is meant to include, but is not limited to, whole blood, serum, plasma, urine, synovial fluid, saliva, cerebrospinal fluid, tissue infiltrate, cervical or vaginal exudate, pleural effusion, bronchioalveolar lavage fluid, gastric lavage fluid, small or large bowel contents, and swab specimens from various bodily orifices dispersed in a suitable medium.
  • the mixture of hybridized probe and PCR product, unhybridized probe and PCR products and excess primers are then added to a microtiter plate coated with an agent capable of binding to the molecule on either the PCR product or the probe.
  • the microtiter plate is coated with streptavidin.
  • a detectably labeled antibody capable of binding to any labeled PCR product which is hybridized to the probe and bound to the plate is then added and the antibody is detected.
  • the antibody is detected colorimetrically or fluorometrically.

Abstract

L'invention concerne un système permettant une quantification des niveaux d'expression d'ARNm dans un échantillon biologique contenant un mélange lysant d'un détergent et d'un chaotrope pour extraire l'ARN total de l'échantillon biologique; un mélange de transcriptase inverse, de nucléotides et d'une amorce oligo-dT pour synthétiser l'ADNc à partir de l'ARNm polyadénylé dans l'ARNm total ; un moyen pour marquer une amorce ou une sonde avec une molécule qui se fixe à un agent appliqué en film sur une plaque de microtitrage; une plaque de microtitrage portant un film d'un agent capable de se fixer à ladite molécule; un moyen pour marquer une amorce ou une sonde avec un marqueur détectable; un anticorps qui détecte l'amorce ou la sonde marquée; et un moyen pour détecter l'anticorps. L'invention concerne également des méthodes de quantification de l'expression de l'ARNm dans un échantillon biologique.
PCT/US1996/008142 1995-06-06 1996-05-31 PROCEDE CHIMIQUE UNIVERSEL DE QUANTIFICATION DE L'EXPRESSION DE L'ARNm UTILISANT UN DOSAGE IMMUNOENZYMATIQUE WO1996039536A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU58851/96A AU5885196A (en) 1995-06-06 1996-05-31 Universal chemistry enzyme-linked immunosorbent assay for de tection of mrna expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46790995A 1995-06-06 1995-06-06
US08/467,909 1995-06-06

Publications (1)

Publication Number Publication Date
WO1996039536A1 true WO1996039536A1 (fr) 1996-12-12

Family

ID=23857641

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/008142 WO1996039536A1 (fr) 1995-06-06 1996-05-31 PROCEDE CHIMIQUE UNIVERSEL DE QUANTIFICATION DE L'EXPRESSION DE L'ARNm UTILISANT UN DOSAGE IMMUNOENZYMATIQUE

Country Status (2)

Country Link
AU (1) AU5885196A (fr)
WO (1) WO1996039536A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2874026A1 (fr) * 2004-08-04 2006-02-10 Centre Nat Rech Scient QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL
WO2010012464A1 (fr) * 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Lyse et transcription inverse améliorées pour la quantification d'arnm
US7972778B2 (en) 1997-04-17 2011-07-05 Applied Biosystems, Llc Method for detecting the presence of a single target nucleic acid in a sample
US8110364B2 (en) 2001-06-08 2012-02-07 Xdx, Inc. Methods and compositions for diagnosing or monitoring autoimmune and chronic inflammatory diseases

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANALYTICAL BIOCHEMISTRY, 1988, Volume 169, MATTHEWS et al., "Review, Analytical Strategies for Use of DNA Probes", pages 1-25. *
MANIATIS et al., "Molecular Cloning, A Laboratory Manual", NEW YORK: COLD SPRING HARBOR LABORATORY, 1982, pages 212-216 and 280-281. *
NUCLEIC ACIDS RESEARCH, 1986, Volume 14, Number 12, SYVANEN et al., "Fast Quantification of Nucleic Acid Hybrids by Affinity-Based Hybrid Collection", pages 5037-5048. *
NUCLEIC ACIDS RESEARCH, 1988, Volume 16, Number 23, SYVANEN et al., "Quantification of Polymerase Chain Reaction Products by Affinity-Based Hybrid Collection", pages 11327-11338. *

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8278071B2 (en) 1997-04-17 2012-10-02 Applied Biosystems, Llc Method for detecting the presence of a single target nucleic acid in a sample
US8551698B2 (en) 1997-04-17 2013-10-08 Applied Biosystems, Llc Method of loading sample into a microfluidic device
US9506105B2 (en) 1997-04-17 2016-11-29 Applied Biosystems, Llc Device and method for amplifying target nucleic acid
US8859204B2 (en) 1997-04-17 2014-10-14 Applied Biosystems, Llc Method for detecting the presence of a target nucleic acid sequence in a sample
US8822183B2 (en) 1997-04-17 2014-09-02 Applied Biosystems, Llc Device for amplifying target nucleic acid
US8067159B2 (en) 1997-04-17 2011-11-29 Applied Biosystems, Llc Methods of detecting amplified product
US8563275B2 (en) 1997-04-17 2013-10-22 Applied Biosystems, Llc Method and device for detecting the presence of a single target nucleic acid in a sample
US8257925B2 (en) 1997-04-17 2012-09-04 Applied Biosystems, Llc Method for detecting the presence of a single target nucleic acid in a sample
US7972778B2 (en) 1997-04-17 2011-07-05 Applied Biosystems, Llc Method for detecting the presence of a single target nucleic acid in a sample
US8110364B2 (en) 2001-06-08 2012-02-07 Xdx, Inc. Methods and compositions for diagnosing or monitoring autoimmune and chronic inflammatory diseases
WO2006024737A2 (fr) * 2004-08-04 2006-03-09 Centre National De La Recherche Scientifique QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL
FR2874026A1 (fr) * 2004-08-04 2006-02-10 Centre Nat Rech Scient QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL
WO2006024737A3 (fr) * 2004-08-04 2006-08-31 Centre Nat Rech Scient QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL
US8623602B2 (en) 2008-08-01 2014-01-07 Roche Diagnostics Operations, Inc. Lysis and reverse transcription for MRNA quantification
WO2010012464A1 (fr) * 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Lyse et transcription inverse améliorées pour la quantification d'arnm
US9714448B2 (en) 2008-08-01 2017-07-25 Roche Molecular Systems, Inc. Lysis and reverse transcription for MRNA quantification

Also Published As

Publication number Publication date
AU5885196A (en) 1996-12-24

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