WO2006024737A3 - QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL - Google Patents

QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL Download PDF

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Publication number
WO2006024737A3
WO2006024737A3 PCT/FR2005/001904 FR2005001904W WO2006024737A3 WO 2006024737 A3 WO2006024737 A3 WO 2006024737A3 FR 2005001904 W FR2005001904 W FR 2005001904W WO 2006024737 A3 WO2006024737 A3 WO 2006024737A3
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WO
WIPO (PCT)
Prior art keywords
quantification
rna
real
time
proteins
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Application number
PCT/FR2005/001904
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English (en)
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WO2006024737A2 (fr
Inventor
Charlotte Behr
Philippe Boeuf
Original Assignee
Centre Nat Rech Scient
Pasteur Institut
Charlotte Behr
Philippe Boeuf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Centre Nat Rech Scient, Pasteur Institut, Charlotte Behr, Philippe Boeuf filed Critical Centre Nat Rech Scient
Publication of WO2006024737A2 publication Critical patent/WO2006024737A2/fr
Publication of WO2006024737A3 publication Critical patent/WO2006024737A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne le domaine de la quantification des ARN dans un échantillon biologique, en particulier la quantification des ARN messagers codant pour des cytokines. La présente invention permet une quantification absolue des taux d'ARN messagers de cytokines humaines présents dans un échantillon biologique, par une technique de RT- PCR en temps réel reposant sur l'utilisation d'amorces hautement spécifiques, la détection des amplifications par incorporation d'un intercalant fluorescent, et la quantification absolue grâce à des standards ARN internes (gènes de ménage) et externes (gamme standard ARN de référence). La présente invention concerne la quantification simultanée d'ARN différents, dans les mêmes conditions expérimentales.
PCT/FR2005/001904 2004-08-04 2005-07-22 QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL WO2006024737A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0408645A FR2874026B1 (fr) 2004-08-04 2004-08-04 QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL
FR0408645 2004-08-04

Publications (2)

Publication Number Publication Date
WO2006024737A2 WO2006024737A2 (fr) 2006-03-09
WO2006024737A3 true WO2006024737A3 (fr) 2006-08-31

Family

ID=34950315

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2005/001904 WO2006024737A2 (fr) 2004-08-04 2005-07-22 QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL

Country Status (2)

Country Link
FR (1) FR2874026B1 (fr)
WO (1) WO2006024737A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112080569B (zh) * 2020-09-30 2022-12-02 北京银丰鼎诚生物工程技术有限公司 用于检测体内移植人源细胞的特异性引物、试剂盒及方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996039536A1 (fr) * 1995-06-06 1996-12-12 T Cell Diagnostics, Inc. PROCEDE CHIMIQUE UNIVERSEL DE QUANTIFICATION DE L'EXPRESSION DE L'ARNm UTILISANT UN DOSAGE IMMUNOENZYMATIQUE
WO1999054510A2 (fr) * 1998-04-23 1999-10-28 Genentech, Inc. Analyse quantitative d'expression de gene
WO2000020629A2 (fr) * 1998-10-05 2000-04-13 Connaught Laboratories Limited Quantification d'arn
WO2003060119A2 (fr) * 2002-01-18 2003-07-24 Universite Libre De Bruxelles Methode permettant de determiner les taux d'acide nucleique in vivo
FR2842211A1 (fr) * 2002-07-09 2004-01-16 Inst Necker Procede et moyens pour l'analyse quantitative du nombre des molecules d'arnm codant pour des genes differents dans des cellules

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996039536A1 (fr) * 1995-06-06 1996-12-12 T Cell Diagnostics, Inc. PROCEDE CHIMIQUE UNIVERSEL DE QUANTIFICATION DE L'EXPRESSION DE L'ARNm UTILISANT UN DOSAGE IMMUNOENZYMATIQUE
WO1999054510A2 (fr) * 1998-04-23 1999-10-28 Genentech, Inc. Analyse quantitative d'expression de gene
WO2000020629A2 (fr) * 1998-10-05 2000-04-13 Connaught Laboratories Limited Quantification d'arn
WO2003060119A2 (fr) * 2002-01-18 2003-07-24 Universite Libre De Bruxelles Methode permettant de determiner les taux d'acide nucleique in vivo
FR2842211A1 (fr) * 2002-07-09 2004-01-16 Inst Necker Procede et moyens pour l'analyse quantitative du nombre des molecules d'arnm codant pour des genes differents dans des cellules

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOEUF PHILIPPE ET AL: "CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use.", BMC IMMUNOLOGY [ELECTRONIC RESOURCE]. 2005, vol. 6, no. 1, 2005, pages 5, XP009067336, ISSN: 1471-2172 *
STORDEUR PATRICK ET AL: "Cytokine mRNA quantification by real-time PCR", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 259, no. 1-2, 1 January 2002 (2002-01-01), pages 55 - 64, XP002322464, ISSN: 0022-1759 *

Also Published As

Publication number Publication date
FR2874026A1 (fr) 2006-02-10
WO2006024737A2 (fr) 2006-03-09
FR2874026B1 (fr) 2006-11-17

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