WO1995022754A1 - Analyseur - Google Patents

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Publication number
WO1995022754A1
WO1995022754A1 PCT/FI1995/000077 FI9500077W WO9522754A1 WO 1995022754 A1 WO1995022754 A1 WO 1995022754A1 FI 9500077 W FI9500077 W FI 9500077W WO 9522754 A1 WO9522754 A1 WO 9522754A1
Authority
WO
WIPO (PCT)
Prior art keywords
light
analysis
prism
well
wells
Prior art date
Application number
PCT/FI1995/000077
Other languages
English (en)
Inventor
Jukka Lekkala
Janusz Sadowski
Harri Joki
Original Assignee
Valtion Teknillinen Tutkimuskeskus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valtion Teknillinen Tutkimuskeskus filed Critical Valtion Teknillinen Tutkimuskeskus
Publication of WO1995022754A1 publication Critical patent/WO1995022754A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons

Definitions

  • the invention relates to a device for carrying out an analysis which device is of the type presented in the preamble of the enclosed claim 1.
  • the drawback with the immunological assay methods is that to generate fluorescence one needs to use specific substances i.e. labels which are capable of generating this phenomenon. Attachment of these substances to the analyte requires an extra step.
  • the purpose of the invention is to present a new kind of device for carrying out the analysis which enables one to utilize the SPR phenomenon without special reagents, yet without changing to any significant degree the structure of the analysis wells and the dosing of the substances to be analyzed into them.
  • the device according to the invention is primarily characterized by what is disclosed in the characterizing portion of the enclosed claim 1.
  • the analysis wells form a multiwell structure with at least one row of wells.
  • the measurement based on the SPR phenomenon is conducted from the direction of the bottom of the structure through a suitable prism structure bordering on the bottom which preferably continues in the longitudinal direction if the row as a structure with a constant perpendicular cross-section.
  • the detectors are also situated on the bottom side of the wells and not on the open side of the wells as in conventional measuring instruments, which utilize a through light beam, like microtiter plates, for example.
  • Fig. 1a-e show examples of various well structures
  • Fig. 2a-c show various alternatives of the measuring principle with the well of the invention
  • Fig. 3 shows a combination of wells
  • Fig. 4a and b show measurements with the combination of wells of Fig. 3,
  • Fig. 5 shows the influence of changes in the concentration to the measured values
  • Fig. 6 shows the influence of biomolecules on the measured values.
  • Figures 1a-1e show an analysis well 1 for immunological assays which includes in a multiwell device of the invention.
  • the result of the immunological reaction taking place in the well can be read optically based on the surface plasmon resonance technique (Surface Plasmon Resonance, SPR) known e.g. from FI patents 85766 and 84940.
  • SPR Surface Plasmon Resonance
  • the well consists of an actual reaction space 2 and a prism 3. Both are made of transparent plastic, e.g polystyrene, by compressing into one unit.
  • a layer 4 of a material capable of generating the SPR phenomenon is attached to the inner surface of the reaction space 2 which also forms one boundary surface of the prism 3 incorporated with the well.
  • This may be a thin film of gold evaporated or sputtered onto the surface.
  • the outer surface of the layer 4 may be formed of a thin additional layer of dielectric material (e.g. polystyrene, glass or diamond) which protects the actual material of the layer 4 and improves attachment of a biomolecule.
  • a biomolecule like antibody, antigen or peptide to attached to the outer surface of the material layer 4, with which the analyte molecule in the sample specifically binds. Coated in this way, the well can be stored in a dry place for a long time.
  • the measurement is performed so that a sample is injected into the well and it is allowed to react with an immobilized molecular layer on the surface of the layer 4.
  • a p-polarized light beam or a luminous beam is incident through the prism 3, which forms the bottom of the well, on the material layer 4 at the boundary surface between the prism 3 and the reaction volume 2.
  • Light is totally reflected back into the prism at the boundary surface at values of the incident angle higher than a specific limiting value of the incident angle provided that the refraction index of the material of the prism 3 is higher than that of the liquid in the reaction space 2.
  • a so-called surface plasmon resonance is observed in the totally reflected light and then the reflected light disappears.
  • the prism 3 forming the bottom of the analysis well 1 can be regarded as a part with a boundary surface 3a, through which the light beam enters the prism, a boundary surface, at which light is totally reflected back into the prism, and a boundary surface 3b, through which the totally reflected light beam leaves the prism.
  • the prism 3 may have a shape of a semicylinder (Fig.1a) or a conventional triangular prism (Fig.1 b) in which case its boundary surfaces 3a and 3b, through which the light beam enters and exits, come closer to each other at the bottom side of the well forming either an apex of a triangle or together a semicircle.
  • the prism may also be a part of aforesaid in which case the outer surface of the bottom of the analysis well is straight being located between the above boundary surfaces 3a, 3b, in other words, it is a kind of truncated prism (Figs. 1c and 1d).
  • Fig.le shows a structure of a prism in which the boundary surfaces 3a, 3b are in the middle of the prism structure coming closer to each other in the direction of the reaction volume 2, in other words, forming a kind of notch in the bottom.
  • the incoming light beam, which is refracted at the boundary surface 3a, is reflected from the outer surface of the prism towards the layer 4 either by virtue of total reflection or by a mirror reflection effected by a reflecting surface 3c attached to the outer surface.
  • the light totally reflected from the boundary surface which borders on the layer 4 is reflected in any of the above ways also from the second outer surface again to the second boundary surface 3b whereat it refracts out of the prism.
  • Figs. 2a-2c show the measuring procedure simplified.
  • the light source 5 is a laser or a light emitting diode (LED). Reflected light can be measured with a photodiode serving as the detector 6 if only changes in the intensity of reflected light at a specific angle (Fig. 2a) are measured.
  • Fig. 2b shows introduction of light with an optical fibre 13 in which case the luminous beam diverging from the tip of the fibre can be made collinear with a collimating optics 7. In an analogical manner, the light from the prism can be led into the detector through an optical fibre 14. If the entire resonance curve is recorded as a function of angle, as shown in Fig.
  • a focusing optics 12 can be used between the light source 5 and the prism 3 and the diverging luminous light beam that has undergone total reflection is measured with an CCD array detector 15 of several detectors.
  • the luminous light beam can be collimated with optics 11 , if necessary, and particularly if the array detector 15 is relatively far from the prism.
  • Figs. 2a-c show a separate optics
  • the optics with the prism 3 in which case the lens structures are located on the surfaces 3a and 3b.
  • the prism and the optics can then be fabricated into a unified structure e.g. by moulding.
  • Figs. 1a-e can be considered to represent various possible well row or strip structures as cross-sections corresponding to the longitudinal direction of the row.
  • the light beam from a photodiode 5 (e.g. a laser diode) is divided e.g. with a holographic element 10 and a collimator associated therewith into collinear light beams incident on each prism 3 of the well plate 9 so that the incident angle is the same in each case, Fig. 4a.
  • Each reflected beam has its own light detector 6 measuring the changes that have taken place in the intensity.
  • the diverging light beam from the photodiode 5 is collimated into a collinear beam by an optics 7.
  • the collinear beams are incident on the entire area of the well plate 9.
  • the light that has undergone total reflection with collinear beams is collected by a two dimensional CCD-detector 15 with adjacent detectors, one for each analysis well 1.
  • a two dimensional CCD-detector 15 with adjacent detectors, one for each analysis well 1.
  • the well plates 9 can also be read, as shown in Fig. 2c, by registering the entire resonance curve as a function of the incident angle for each well 1.
  • the optical setup is then the K ⁇ hler illumination known per se.
  • the light beams can be directed to a row of prisms 3 forming a continuous structure from e.g. a row of several light sources 5.
  • the detectors 6 can be in a row and, when a CCD detector 15 is used, it needs to be only a one-dimensional detector. If it is desired to register the entire resonance curve as a function of the incident angle, a setup according to Fig. 2c can be used having a row of light sources 5 and a two-dimensional detector of several detectors as the CCD detector 15.
  • the SPR cuvette and well plate presented here enables a faster determination because no labelling (additional reagents) is needed.
  • the only additional step possibly needed is the washing of the SPR cuvette before measurement for removing the non-specifically bound material.
  • a simple measuring procedure also enables development of cheap portable measuring instruments e.g. for environmental analytics.
  • the light from the light source must be p-polarized. If the light source itself does not have a polarizer, this can be realized by a separate polarizer placed between the light source and the prism shown under the reference numeral 16 in Figs. 2b, 2c, and 4b.
  • the strip or the well plate multiple sample measurement as well as a positive and a negative control sample can be used for checking the result.
  • the strips or the plates may also have separate control coatings in a sealed space for calibrating the measurement.
  • the well strips and the well plates can be fabricated so that their external dimensions are the same as those of existing strips and plates. Thus new dosing and incubation equipment are not needed.
  • the above structures can be fabricated by spray moulding using plastic material of appropriate optical quality, after which the bottoms of the wells 1 can be coated with a SPR material layer 4 using some known technique.
  • the well plate 9 can be manufactured also by moulding from a single piece thereby forming, in a corresponding fashion, prism structures which are parallel with the well rows and which have a constant cross-section.
  • Fig. 5 shows the influence of a NaCI dilution series 300-80 mM on the intensity of the light obtained in the analysis.
  • concentrations of the liquid in the reaction space which borders on the thin layer of gold, has a clear influence on the light intensity.
  • the measurement has been conducted on the downwards sloping part of the curve representing the intensity as a function of the incident angle in which case an increase in the concentration at a specific constant value of the incident angle effecting the SPR phenomenon causes shift of the curve to the right and, at the same time, an increase in the intensity values of the light that has undergone the SPR phenomenon.
  • Fig. 6 shows the influence of binding of a synthetic peptide and HIV antibody on the intensity values.
  • the influence of the above actions and binding between biomolecules on the intensity values of the SPR measurement can be clearly observed and the figure is a good example how the reaction in the reaction space 2 or in several reaction spaces 2 can be continuously monitored in the device according to the invention.
  • the purpose of use of the invention is primarily the monitoring of the reactions between biomolecules and their qualitative and quantitative analysis, the invention can be applied in all kinds of analyses in which a change in the analyte in the reaction space causes a sufficiently clear change in the light that has gone through the prism.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)

Abstract

Un analyseur se compose d'un tube d'analyse (1) destiné aux échantillons à analyseur, d'une source lumineuse (5) dirigée vers le tube d'analyse et d'un capteur (6) recevant le pinceau lumineux issu du pinceau lumineux dirigé par la source lumineuse vers le tube d'analyse (1). Le fond du volume de réaction (2) du tube d'analyse est revêtu d'une couche (4) d'une substance capable de produire un phénomène de résonance de plasmon de surface ou 'phénomène SPR' (Surface Plasmon Resonance), et éventuellement pourvue d'un revêtement supplémentaire. Le fond du tube d'analyse correspondant aux limites de la couche (4) est transparent à la lumière qui, grâce à ladite substance, produit le phénomène SPR. Ce fond en forme de prisme (3) comporte un dioptre (3a) d'admission de lumière, un dioptre (3b) de réémission de lumière et un dioptre assurant une réflexion totale. La source lumineuse (5) dirige son pinceau lumineux au travers du prisme (3) vers l'espace de réaction (2), le capteur (6) étant en position de réception de la lumière renvoyée par le prisme (3). Les tubes d'analyse (1) forment un assemblage de plusieurs tubes, lequel assemblage est en forme de bande ou de plaquette à microtitrage.
PCT/FI1995/000077 1994-02-16 1995-02-16 Analyseur WO1995022754A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI940737A FI96800C (fi) 1994-02-16 1994-02-16 Laite analyysin suorittamiseksi
FI940737 1994-02-16

Publications (1)

Publication Number Publication Date
WO1995022754A1 true WO1995022754A1 (fr) 1995-08-24

Family

ID=8540132

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI1995/000077 WO1995022754A1 (fr) 1994-02-16 1995-02-16 Analyseur

Country Status (2)

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FI (1) FI96800C (fr)
WO (1) WO1995022754A1 (fr)

Cited By (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040366A1 (fr) * 1996-04-19 1997-10-30 Carl Zeiss Jena Gmbh Procede et dispositif permettant de mettre en evidence des reactions et des interactions d'ordre physique, chimique, biologique ou biochimique
EP0935131A2 (fr) * 1998-02-05 1999-08-11 Fuji Photo Film Co., Ltd. Capteur à résonance de plasmons de surface avec source de lumière laser stabilisée en longueur d'onde
DE19817470A1 (de) * 1998-04-20 1999-10-21 Biotul Bio Instr Gmbh Transducer-Anordnung für einen optischen Sensor basierend auf der Oberflächenplasmonenresonanz
WO1999060382A1 (fr) * 1998-05-20 1999-11-25 Graffinity Pharmaceutical Design Gmbh Capteur de résonance de plasmons superficiels pour la mesure simultanee d'une pluralite d'echantillons sous forme fluide
EP0973023A1 (fr) * 1998-07-14 2000-01-19 Texas Instruments Incorporated Système d'analyse à haut débit de résonance de plasmons de surface
WO2000014514A1 (fr) * 1998-09-08 2000-03-16 Motorola, Inc. Analyseur de biomolecules comprenant une mosaique de detecteurs et un filtre
WO2000022419A1 (fr) * 1998-04-02 2000-04-20 Institut für Physikalische Hochtechnologie e.V. Dispositif pour la spectroscopie par resonance des plasmons de surface
WO2000031515A1 (fr) * 1998-11-20 2000-06-02 Graffinity Pharmaceutical Design Gmbh Systeme de mesure pour selection parallele de detecteurs par resonance plasmonique de surface (spr)
EP1038167A1 (fr) * 1997-12-12 2000-09-27 The Perkin-Elmer Corporation Systeme d'analyse par resonance optique
EP1079226A1 (fr) * 1999-08-24 2001-02-28 Leuze electronic GmbH + Co. Dispositif de dosage immunologique
WO2001059403A1 (fr) * 2000-02-11 2001-08-16 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Procede de determination quantitative et/ou qualitative d'epaisseurs de couche, contenant pour microreactions et plaque de titration
WO2001063257A1 (fr) * 2000-02-22 2001-08-30 Graffinity Pharmaceutical Design Gmbh Detecteur spr et dispositif de detection spr
WO2001063256A1 (fr) * 2000-02-22 2001-08-30 Graffinity Pharmaceutical Design Gmbh Systeme de detection par resonance plasmonique de surface
EP1154259A1 (fr) 2000-05-11 2001-11-14 Fuji Photo Film Co., Ltd. Plaque de mesure
EP1172644A1 (fr) * 2000-07-11 2002-01-16 Suzuki Motor Corporation Guide d'ondes pour SPR et l'utilisation dans un appareil pour la mesure des réactions immunologiques
WO2002039095A1 (fr) * 2000-11-10 2002-05-16 Jandratek Gmbh Detecteur a resonance au plasmon, en particulier pour biocaptage
EP1243916A2 (fr) * 2001-03-22 2002-09-25 Fuji Photo Film Co., Ltd. Appareil et puce de mesure
EP1251345A1 (fr) * 2001-04-12 2002-10-23 Fuji Photo Film Co., Ltd. Senseur de mesure avec le principe de la réflection totale réduite
WO2003014715A1 (fr) * 2001-08-06 2003-02-20 Cambridge Consultants Limited Interferometre de detection par resonance plasmonique de surface
WO2003046198A2 (fr) * 2001-11-28 2003-06-05 Graffinity Pharmaceuticals Ag Procede de selection et d'identification de molecules peptidiques ou proteiques par presentation a la surface de phages
WO2003046526A1 (fr) * 2001-11-28 2003-06-05 Graffinity Pharmaceuticals Ag Support de surface de detecteur spr
EP1371966A1 (fr) * 2002-06-14 2003-12-17 Stiftung Für Diagnostische Forschung Cuvette pour un dispositif de lecteur pour mettre en évidence des substances selon la technique du champ évanescent
WO2004008120A1 (fr) * 2002-07-10 2004-01-22 E2V Technologies (Uk) Limited Agencement de detecteur moleculaire
JP2004020267A (ja) * 2002-06-13 2004-01-22 Fuji Photo Film Co Ltd 測定装置および該測定装置の使用方法
DE19806681B4 (de) * 1998-02-18 2006-07-27 Carl Zeiss Jena Gmbh Mikrotiterplatte
JP2009042164A (ja) * 2007-08-10 2009-02-26 Hitachi Maxell Ltd 赤外線カメラ
WO2011081847A1 (fr) * 2009-12-30 2011-07-07 Maven Technologies, Llc. Test biologique avec un substrat ayant une surface inférieure comprenant des prismes en dents de scie
US7976217B2 (en) 2006-09-15 2011-07-12 Corning Incorporated Screening system and method for analyzing a plurality of biosensors
US8114348B2 (en) 2005-07-20 2012-02-14 Corning Incorporated Label-free high throughput biomolecular screening system and method
EP2761274A4 (fr) * 2011-09-28 2015-06-03 Ge Healthcare Bio Sciences Ab Système de biocapteur par résonance des plasmons de surface
JP2015121552A (ja) * 2006-12-12 2015-07-02 コーニンクレッカ フィリップス エヌ ヴェ ラベル粒子を検出するマイクロエレクトロニクスセンサデバイス
JP2019520549A (ja) * 2016-05-03 2019-07-18 ベンタナ メディカル システムズ， インコーポレイテッド 試薬濃度をモニタリングするシステムおよび方法

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CN104198440B (zh) * 2014-08-29 2016-06-08 西安交通大学 一种便携探入式表面等离子体共振生物传感器及其制备和检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982000359A1 (fr) * 1980-07-24 1982-02-04 Oy Labsystems Ensemble de cuvettes
EP0286195A2 (fr) * 1987-04-10 1988-10-12 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Méthode et appareil pour détecter des concentrations faibles de composés chimiques ou biologiques dans un milieu d'examen en utilisant la résonance par plasmons de surface
DE3830002A1 (de) * 1988-08-31 1990-03-01 Naumann Dieter Vorrichtung fuer eine bewegliche kuevette fuer vergleichende reflektionsspektroskopische messungen
EP0517930A1 (fr) * 1991-06-08 1992-12-16 Hewlett-Packard GmbH Procédé et appareil pour détecter la présence et/ou la concentration de biomolécules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982000359A1 (fr) * 1980-07-24 1982-02-04 Oy Labsystems Ensemble de cuvettes
EP0286195A2 (fr) * 1987-04-10 1988-10-12 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Méthode et appareil pour détecter des concentrations faibles de composés chimiques ou biologiques dans un milieu d'examen en utilisant la résonance par plasmons de surface
DE3830002A1 (de) * 1988-08-31 1990-03-01 Naumann Dieter Vorrichtung fuer eine bewegliche kuevette fuer vergleichende reflektionsspektroskopische messungen
EP0517930A1 (fr) * 1991-06-08 1992-12-16 Hewlett-Packard GmbH Procédé et appareil pour détecter la présence et/ou la concentration de biomolécules

Cited By (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19615366B4 (de) * 1996-04-19 2006-02-09 Carl Zeiss Jena Gmbh Verfahren und Einrichtung zum Nachweis physikalischer, chemischer, biologischer oder biochemischer Reaktionen und Wechselwirkungen
WO1997040366A1 (fr) * 1996-04-19 1997-10-30 Carl Zeiss Jena Gmbh Procede et dispositif permettant de mettre en evidence des reactions et des interactions d'ordre physique, chimique, biologique ou biochimique
US5999262A (en) * 1996-04-19 1999-12-07 Carl Zeiss Jena Gmbh Process and apparatus for detecting structural changes of specimens
EP1038167A4 (fr) * 1997-12-12 2001-08-22 Perkin Elmer Corp Systeme d'analyse par resonance optique
EP1038167A1 (fr) * 1997-12-12 2000-09-27 The Perkin-Elmer Corporation Systeme d'analyse par resonance optique
EP0935131A2 (fr) * 1998-02-05 1999-08-11 Fuji Photo Film Co., Ltd. Capteur à résonance de plasmons de surface avec source de lumière laser stabilisée en longueur d'onde
DE19806681B4 (de) * 1998-02-18 2006-07-27 Carl Zeiss Jena Gmbh Mikrotiterplatte
WO2000022419A1 (fr) * 1998-04-02 2000-04-20 Institut für Physikalische Hochtechnologie e.V. Dispositif pour la spectroscopie par resonance des plasmons de surface
US6570657B1 (en) 1998-04-02 2003-05-27 Institut Fuer Physikalische Hochtechnolgolie E.V. Arrangement for surface plasmon resonance spectroscopy
DE19817470A1 (de) * 1998-04-20 1999-10-21 Biotul Bio Instr Gmbh Transducer-Anordnung für einen optischen Sensor basierend auf der Oberflächenplasmonenresonanz
DE19817470B4 (de) * 1998-04-20 2008-10-30 Hofmann, Andreas Vorrichtung zur Oberflächenplasmonenresonanzmessung
US6373577B1 (en) 1998-05-20 2002-04-16 Graffinity Pharmaceutical Design Gmbh Surface plasmon resonance sensor for the simultaneous measurement of a plurality of samples in fluid form
WO1999060382A1 (fr) * 1998-05-20 1999-11-25 Graffinity Pharmaceutical Design Gmbh Capteur de résonance de plasmons superficiels pour la mesure simultanee d'une pluralite d'echantillons sous forme fluide
US6111652A (en) * 1998-07-14 2000-08-29 Texas Instruments Incorporated High throughput surface plasmon resonance analysis system
EP0973023A1 (fr) * 1998-07-14 2000-01-19 Texas Instruments Incorporated Système d'analyse à haut débit de résonance de plasmons de surface
WO2000014514A1 (fr) * 1998-09-08 2000-03-16 Motorola, Inc. Analyseur de biomolecules comprenant une mosaique de detecteurs et un filtre
WO2000031515A1 (fr) * 1998-11-20 2000-06-02 Graffinity Pharmaceutical Design Gmbh Systeme de mesure pour selection parallele de detecteurs par resonance plasmonique de surface (spr)
DE19955556B4 (de) * 1998-11-20 2004-09-09 Graffinity Pharmaceuticals Ag Meßanordnung zum parallelen Auslesen von SPR-Sensoren
US6441906B2 (en) 1998-11-20 2002-08-27 Graffinity Pharmaceutical Design Gmbh Set-up of measuring instruments for the parallel readout of SPR sensors
US6929943B1 (en) 1999-08-24 2005-08-16 Leuze Electronic Gmbh & Co. Device for analyzing immunoassays
EP1079226A1 (fr) * 1999-08-24 2001-02-28 Leuze electronic GmbH + Co. Dispositif de dosage immunologique
WO2001059403A1 (fr) * 2000-02-11 2001-08-16 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Procede de determination quantitative et/ou qualitative d'epaisseurs de couche, contenant pour microreactions et plaque de titration
US7396684B2 (en) 2000-02-11 2008-07-08 Institut Fur Mikrotechnik Mainz Gmbh Method for quantitatively and/or qualitatively detecting layer thicknesses, a microreaction vessel and titre plate
WO2001063256A1 (fr) * 2000-02-22 2001-08-30 Graffinity Pharmaceutical Design Gmbh Systeme de detection par resonance plasmonique de surface
WO2001063257A1 (fr) * 2000-02-22 2001-08-30 Graffinity Pharmaceutical Design Gmbh Detecteur spr et dispositif de detection spr
US6752963B2 (en) 2000-02-22 2004-06-22 Graffinity Pharmaceutical Design Gmbh SPR sensor system
AU2001246447B2 (en) * 2000-02-22 2005-12-08 Graffinity Pharmaceutical Design Gmbh Spr sensor system
US6795192B2 (en) 2000-02-22 2004-09-21 Graffinity Pharmaceutical Design Gmbh SPR sensor and SPR sensor array
US6597456B2 (en) 2000-05-11 2003-07-22 Fuji Photo Film Co., Ltd. Measuring chip for quantitative analysis of substances
EP1154259A1 (fr) 2000-05-11 2001-11-14 Fuji Photo Film Co., Ltd. Plaque de mesure
US6507402B2 (en) 2000-07-11 2003-01-14 Suzuki Motor Corporation SPR sensor plate and immune reaction measuring instrument using the same
EP1172644A1 (fr) * 2000-07-11 2002-01-16 Suzuki Motor Corporation Guide d'ondes pour SPR et l'utilisation dans un appareil pour la mesure des réactions immunologiques
WO2002039095A1 (fr) * 2000-11-10 2002-05-16 Jandratek Gmbh Detecteur a resonance au plasmon, en particulier pour biocaptage
US6831748B2 (en) * 2000-11-10 2004-12-14 Jandratek Gmbh Plasmon resonance sensor, especially for use in biosensor technology
US7075654B2 (en) 2001-03-22 2006-07-11 Fuji Photo Film Co., Ltd. Measuring apparatus and measuring chip
US7030988B2 (en) 2001-03-22 2006-04-18 Fuji Photo Film Co., Ltd. Measuring apparatus and measuring chip
EP1243916A3 (fr) * 2001-03-22 2004-04-14 Fuji Photo Film Co., Ltd. Appareil et puce de mesure
EP1243916A2 (fr) * 2001-03-22 2002-09-25 Fuji Photo Film Co., Ltd. Appareil et puce de mesure
EP1251345A1 (fr) * 2001-04-12 2002-10-23 Fuji Photo Film Co., Ltd. Senseur de mesure avec le principe de la réflection totale réduite
US7064837B2 (en) 2001-04-12 2006-06-20 Fuji Photo Film Co., Ltd. Measuring sensor utilizing attenuated total reflection and measuring chip assembly
US7084980B2 (en) 2001-08-06 2006-08-01 Cambridge Consultants Limited SPR interferometer
WO2003014715A1 (fr) * 2001-08-06 2003-02-20 Cambridge Consultants Limited Interferometre de detection par resonance plasmonique de surface
WO2003046526A1 (fr) * 2001-11-28 2003-06-05 Graffinity Pharmaceuticals Ag Support de surface de detecteur spr
WO2003046198A2 (fr) * 2001-11-28 2003-06-05 Graffinity Pharmaceuticals Ag Procede de selection et d'identification de molecules peptidiques ou proteiques par presentation a la surface de phages
WO2003046198A3 (fr) * 2001-11-28 2004-03-25 Graffinity Pharmaceuticals Ag Procede de selection et d'identification de molecules peptidiques ou proteiques par presentation a la surface de phages
JP2004020267A (ja) * 2002-06-13 2004-01-22 Fuji Photo Film Co Ltd 測定装置および該測定装置の使用方法
AU2003204706B2 (en) * 2002-06-14 2004-05-20 Leuze Electronic Gmbh + Co. A Cuvette for a Reader Device for Assaying Substances Using the Evanescence Field Method
EP1371966A1 (fr) * 2002-06-14 2003-12-17 Stiftung Für Diagnostische Forschung Cuvette pour un dispositif de lecteur pour mettre en évidence des substances selon la technique du champ évanescent
EP1371967A1 (fr) * 2002-06-14 2003-12-17 Stiftung Für Diagnostische Forschung Cuvette pour un dispositif de lecteur pour mettre en évidence des substances selon la technique du champ évanescent
WO2004008120A1 (fr) * 2002-07-10 2004-01-22 E2V Technologies (Uk) Limited Agencement de detecteur moleculaire
US8114348B2 (en) 2005-07-20 2012-02-14 Corning Incorporated Label-free high throughput biomolecular screening system and method
US7976217B2 (en) 2006-09-15 2011-07-12 Corning Incorporated Screening system and method for analyzing a plurality of biosensors
US8231268B2 (en) 2006-09-15 2012-07-31 Corning Incorporated Screening system and method for analyzing a plurality of biosensors
JP2015121552A (ja) * 2006-12-12 2015-07-02 コーニンクレッカ フィリップス エヌ ヴェ ラベル粒子を検出するマイクロエレクトロニクスセンサデバイス
JP2017021050A (ja) * 2006-12-12 2017-01-26 コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． マイクロエレクトロニクスセンサデバイス、読み取り装置及び検出方法
US11243199B2 (en) 2006-12-12 2022-02-08 Siemens Healthineers Nederland B.V. Carrier for detecting label particles
US11402374B2 (en) 2006-12-12 2022-08-02 Siemens Healthineers Nederland B.V. Method of detecting label particles
JP2009042164A (ja) * 2007-08-10 2009-02-26 Hitachi Maxell Ltd 赤外線カメラ
WO2011081847A1 (fr) * 2009-12-30 2011-07-07 Maven Technologies, Llc. Test biologique avec un substrat ayant une surface inférieure comprenant des prismes en dents de scie
US8355133B2 (en) 2009-12-30 2013-01-15 Maven Technologies, Llc Biological testing with sawtooth-shaped prisms
EP2761274A4 (fr) * 2011-09-28 2015-06-03 Ge Healthcare Bio Sciences Ab Système de biocapteur par résonance des plasmons de surface
US10768108B2 (en) 2011-09-28 2020-09-08 Ge Healthcare Bio-Sciences Ab Surface plasmon resonance biosensor system
JP2019520549A (ja) * 2016-05-03 2019-07-18 ベンタナ メディカル システムズ， インコーポレイテッド 試薬濃度をモニタリングするシステムおよび方法

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FI940737A0 (fi) 1994-02-16
FI96800B (fi) 1996-05-15
FI940737A (fi) 1995-08-17

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