Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
  • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
  • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
  • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
  • C07D239/46—Two or more oxygen, sulphur or nitrogen atoms
  • C07D239/47—One nitrogen atom and one oxygen or sulfur atom, e.g. cytosine
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
  • A61P31/14—Antivirals for RNA viruses
  • A61P31/18—Antivirals for RNA viruses for HIV
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
  • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
  • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
  • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
  • C07D239/46—Two or more oxygen, sulphur or nitrogen atoms
  • C07D239/56—One oxygen atom and one sulfur atom
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
  • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
  • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
  • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
  • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
  • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
  • C07D409/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings
  • C07D409/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
  • C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
  • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
  • C07D471/04—Ortho-condensed systems

Definitions

• the pyrimidine-thioalkyl and alkylether derivatives of Formula IA are useful in the treatment of individuals who are HIV positive, whether or not they show AIDS symptoms at the present time.
• the pyrimidine-thioalkyl and alkylether derivatives of Formula IB are useful in the preparation of the pyrimidine-thioalkyl and alkylether derivatives of Formula IA.
• R j to R 4 independently of one another, represent hydrogen, lower alkyl, halogen, amino or hydroxy groups
• R 5 represents a free electron pair or a lower alkyl group
• a halogen atom m has the value 0 or 1
• the pyrimidine-thioalkyl group being bonded in the 2-, 3- or 4- position of the pyridine ring, and to therapeutically compatible acid addition salts thereof.
• the compounds allegedly exhibit surprisingly improved bronchosecretolytic and myucolytic activity as well as having been found to show antiphlogistic activity.
• J. Med Chem. 1987, 30, 547-551 describes various 2-[(pyridinylmethyl)thio]-pyrimidine derivatives and the influence thereof on bronchosecretolytic properties in the phenol red screening model of the mouse in comparison to the known drug ambroxol.
• EP 477 778 (Derwent 92-106190/14) describes various benzene, pyridine and pyrimidine derivatives as ACAT enzyme inhibitors, for treating arteriosclerosis, and cerebrovascular disease.
• J. Org. Chem, 1954, 19, 1793-1801 describes pyrimidine derivatives, including 2-benzylmercapto-4-amino-6-pyrimidinol, 2-benzylmercapto-4-amino-6-chloropyrimidine, 2-benzylmercapto-4-amino-6-diethylaminopyrimidine as well as analogs of 6- dimethylaminopurine.
• HIV-1 human immunodeficiency virus type I
• AZT zidovudine
• U.S. Patent 4,724,232 claims a method of treating humans having acquired immunodeficiency syndrome utilizing 3'-azido-3'-deoxy-thymidine (azidothymidine, AZT).
• J. Org. Chem. 1962, 27, 181-185 describes various 2-benzylthio pyrimidine derivatives, including 4-chloro-5-methyl-2-[(phenylmethyl)thio]-pyrimidine, 4-chloro-5-methyl-2-[[(2,4- dichloro-phenyl)methyl] thio] -pyrimidine, 4-chloro-5-methyl-2- [ [(2-chloro-phenyl)methyl] thio] - pyrimidine, and 4-chloro-5-methyl-2-[[(4-chloro-phenyl)methyl]thio]-pyrimidine and their activity as antitumor compounds in screens against SA-180, CA 755, and L-1210 tumor systems.
• J. Med. Chem. 1977, 20, 88-92 describes 2-alkoxy and 2-alkylthio-4-amino pyrimdines, including 2-[(phenylmethyl)thio]4-pyrimidinamine, 2-[[(4-chlorophenyl)methyl]thio]-4- pyrimidinamine, 2-[(3-pyridinylmethyl)thio]4-pyrimidinamine, and 2-(phenylmethoxy)-4- pyrimidinamine, and their activity as inhibitors of deoxycytidine kinase. Collect. Czech. Chem. Comm. 1975, 40, 1078-1088 (CA 83: 114326e) describes
• the compounds corresponding to Formula I may exist in various tautomeric formulas, and are included within the scope of Formula I as well as Formula IA and IB.
• n 0 or 1
• R 1 is selected from the group consisting of -C ⁇ CH,
• R 20 , R 21 , R 22 , R 3 , 24> and R 25 are the same or different and are selected from -H, C j -C 6 alkyl, C C 6 alkenyl, C j -C 6 alkoxy, C j -C 6 alkylthio, -C 3 -C 8 cycloalkyl, -CF 3 , -N0 2 , -halo, -OH, -CN, phenyl, phenylthio, -styryl, -C0 2 (R 31 ), -CON(R 31 )(R 32 ), -CO(R 31 ), -(CH 2 ) n -N(R 31 )(R 32 ), -C(OH)(R 31 )(R 33 ), -(CH 2 ) n N(R 31 )(CO(R 3 3)), -(CH 2 ) n N(R 31 )(CO(R 3 3)), -
• C r C 6 alkyl phenyl optionally substituted with 1, 2, or 3 -halo, C r C 6 alkyl, -,-C 6 alkoxy, -CF 3 , -N0 2 , -OH, -CN), or where R 31 and R 32 taken together with the attached nitrogen to form a ring selected from -pyrrolidinyl, -piperidinyl, -4-morpholinyl, -4-thiomorpholinyl, -4-piperazinyl, -4-(l-C j -C 6 alkyl)piperazinyl, or a member selected from the group consisting of:
• Y is selected from -S-, -S(O)-, -S(0) 2 , or -0-;
• R 4 is selected from the group consisting of -H, -OH, halo or -NR 15 R 16 where R 15 is -H and R 16 is -H, C j -C 6 alkyl, -NH 2 or R 15 and R 16 taken together with the -N form 1-pyrrolidino, 1-morpholino or 1-piperidino;
• R 5 is selected from the group consisting of -H, halo, cyclohexyl, C j -C 4 alkyl or C j -C 3 alkoxy;
• R 6 is selected from the group consisting of -H, -OH or halo (preferably -CI), with the overall provisio that R and R 6 are not both -H; and pharmaceutically acceptable salts, hydrates, N-oxides and solvates thereof.
• An embodiment of the present invention are pyrimidine-thioalkyl and alklyether anti-
• R 4 is selected from the group consisting of -H or -NR 15 R 16 where R 15 is -H and R 16 is -H, C r C 6 alkyl, -NH 2 or R 15 and R 16 taken together with the -N form 1-pyrrolidino, 1-morpholino or 1-piperidino; and R 6 is selected from the group consisting of -H or halo (preferably -CI).
• An embodiment of the present invention are compounds of Formula I (as well as Formula IA and IB) where Y is -O-.
• a preferred embodiment of the present invention are compounds of Formula I (as well as Formula IA and IB) where s is 0 and Y is selected from the group consisting of -S-, -S(O)- or -S(0) 2 ; more preferably Y is -S-.
• R 4 is preferably -NH 2 .
• m is preferably 0.
• R 6 is preferably halo, more preferably -CI.
• R 1 is preferably selected from the group consisting of phenyl optionally substituted with one, 2 or 3 C r C 4 alkyl, C j -C 3 alkoxy, halo, C C 3 alkylthio, trifluoromethyl,
• Novel pyrimidine-thioalkyl and alkylether anti-AIDS compounds of Formula IA include compounds where R 4 is selected from the group consisting of -H or -NR 15 R 16 where R 15 is -H and R 16 is
• R 1 is selected from the group consisting of phenyl optionally substituted with one, 2 or 3 C j -C 4 alkyl, C j -C 3 alkoxy, halo, C j -C 3 alkylthio, trifluoromethyl, C 2 -C 6 dialkylamino, or nitro; naphthyl optionally substituted with one or 2 C r C 4 alkyl, C r C 3 alkoxy, halo, trifluoromethyl, C 2 -C 6 dialkylamino, C j -C j alkylthio or nitro; -C ⁇ CH; or a member selected from the group consisting of:
• Preferred novel pyrimidine-thioalkyl and alkylether anti-AIDS compounds of Formula IA include compounds where
• R 4 is selected from the group consisting of -H or -NR 15 R 16 where R 15 is -H and R 16 is -H, -NH 2 or R 15 and R 16 taken together with the -N form 1-pyrrolidino, 1-morpholino or 1-piperidino; and
• R 1 is phenyl optionally substituted with one, 2 or 3 C r C 4 alkyl, C r C 3 alkoxy, halo, C j -C 3 alkylthio, trifluoromethyl, C 2 -C 6 dialkylamino, or nitro; other than 4-amino-6-chloro-2-(benzylthio)-pyrimidine, 4-amino-6-hydroxy-2-(benzylthio)-pyrimidine,
• IA include compounds where
• R 4 is selected from the group consisting of -H or -NR 15 R 16 where R 15 is -H and R 16 is -H, -NH 2 or R 15 and R j6 taken together with the -N form 1-pyrrolidino, 1-morpholino or 1-piperidino.
• the pyrimidine-thioalkyl compounds of Formula I are generally and most often prepared by contacting a 2-mercaptopyrimidine with an appropriate halide.
• the pyrimidine-thioalkyl and alkylether compounds of Formula I include the compounds of EXAMPLES 1-167. Preferred are the novel anti-AIDS compounds of EXAMPLES 36, 43, 59, 64, 114 and 132 as well as the intermediate compounds of EXAMPLES 4, 9-17, 26, 27, 32, 74, 76, 82 and 110. Preferred prior art compounds useful as anti-AIDS compounds are the compounds of EXAMPLES 55, and 71. Compound 132 is a particularly preferred anti-AIDS compound of the subject invention.
• the pyrimidine-thioalkyl and alkylether compounds of Formula I form acid addition salts, some of the variable substituents are acids and as such form base addition salts when reacted with bases of sufficient strength.
• the pharmaceutically acceptable salts include both inorganic and organic bases.
• the preferred pharmaceutically acceptable salts include salts of the following bases, for example, hydroxide, ammonia, tromethamine (THAM), 2-amino-2- (hydroxymethyl)-l,3-propanediol.
• Suitable cations include, for example, sodium, potassium, calcium and magnesium.
• the pyrimidine-thioalkyl and alkylether anti-AIDS compounds of Formula IA are useful as inhibitors of viral reverse transcriptase, an enzyme necessary for human immunodeficiency virus replication and therefore would be useful in the treatment of such diseases as AIDS.
• human retrovirus indicates human immunodeficiency virus type I, or strains thereof apparent to one skilled in the art, which belong to the same viral families and which create similar physiological effects in humans as various human retro viruses.
• Patients to be treated would include those individuals (1) infected with one or more than one strain of a human retrovirus as determined by the presence of either measurable viral antibody or antigen in the serum and (2) having either a symptomatic AIDS defining infection such as (a) disseminated histoplasmosis, (b) isopsoriasis, (c) bronchial and pulmonary candidiasis including pneumocystic pneumonia (d) non-Hodgkin's lymphoma or (e) Kaposi's sarcoma and being less than sixty years old; or having an absolute CD4 lymphocyte count of less than 200/mm 3 in the peripheral blood.
• a symptomatic AIDS defining infection such as (a) disseminated histoplasmosis, (b) isopsoriasis, (c
• the compounds of Formula IA can be given orally.
• Suitable dosage forms include tablets, capsules, suspensions, solutions and elixirs.
• An effective amount is from about 0.1 to about 500 mg/kg/day.
• a typical unit dose for a 70 kg human would be from about 10 mg to about 2000 mg, preferably about 100 mg to about 1000 mg taken one to six times per day.
• the exact dosage and frequency of administration depends on the particular compound of Formula IA used, the particular condition being treated, the severity of the condition being treated, the age, weight, general physical condition of the particular patient, other medication the individual may be taking as is well known to those skilled in the art and can be more accurately determined by measuring the blood level or concentration of the compounds of Formula IA in the patient's blood and/or the patient's response to the particular condition being treated.
• ARC HIV-related complex
• AIDS patients would typically be treated with higher oral doses (about 1 to about 500 mg/kg/day).
• the pyrimidine-thioalkyl and alkylether anti-AIDS compounds of Formula IA of this invention can be used in conjunction with other antiviral agents such as AZT, ddl, ddC, with non-nucleoside anti-AIDS agents such as those disclosed in International Publication No. WO91/09849, published July 11, 1991, and International Publication No. WO93/01181, published January 21, 1993, and with protease inhibitors.
• the utility of the pyrimidine-thioalkyl and alkylether anti-AIDS compounds of Formula IA of this invention can be determined by their ability to inhibit viral reverse transcriptase, an enzyme essential for human immunodeficiency virus replication.
• This enzyme has charac ⁇ teristics which differentiate it from other known cellular polymerases and it is a unique enzyme which is not found in uninfected cells.
• Viral reverse transcriptase is found in extracts from bacterial clones prepared according to the procedure described in AIDS Virus Reverse Transcriptase defined by high level expression in Escherichia coli, EMBO J. 6:3133-3137
• P236L viral reverse transcriptase is obtained by PNAS 90: 4713-4717 (1993). Inhibition of this enzyme is determined in a cell free assay which measures the level of radioactive precur ⁇ sors incorporated into DNA. Extracts prepared according to the procedure of Science, 1125- 1129 (1981) are incubated in a mixture of inhibitor, 20 mM dithiothreitol, 60 mM sodium chlor- ide, 0.05% NP-40, 10 mM magnesium chloride, 50 mM Tris pH 8.3, 10 ⁇ M [ 35 S]-labeled deoxynuleoside-5'-triphosphate, 10 ⁇ g/ml RNA template (poly rC or poly rG) and 5 ⁇ g/ml DNA primer (oligo dG or oligo dT) for 30 minutes at 37°C.
• IC 50 means the concentration, in ⁇ M of drug, required to inhibit the reverse transcriptase activity (P236L) to the extent of 50%) of various assay(s) are combined and reported as % inhibition and or IC 50 (calculated) in Table I.
• variable substituents contained in parentheses are bonded to the atom immediately to the left of the variable substituent enclosed in parenthesis.
• each of the consecutive variable substituents is bonded to the immediately preceding atom to the left which is not enclosed in parentheses.
• both R j and R are bonded to the preceding carbon atom.
• Chemical formulas or portions thereof drawn in a linear fashion represent atoms in a linear chain.
• the symbol "-" in general represents a bond between two atoms in the chain.
• CH 3 -0-CH 2 -CH(R j )-CH 3 represents a 2-substituted-l-methoxypropane compound.
• the symbol " ⁇ " represents a triple bond, e.g., HC ⁇ C-CH(R i )-CH 2 -CH 3 .
• Chemical formulas of cyclic (ring) compounds or molecular fragments can be represented in a linear fashion.
• the cyclic molecular fragment, 4-(ethyl)-l-piperazinyl can be represented by -N*-(CH 2 ) 2 -N(C 2 H 5 )-CH 2 -C*H 2 .
• a rigid cyclic (ring) structure for any compounds herein defines an orientation with respect to the plane of the ring for substituents attached to each carbon atom of the rigid cyclic compound.
• the two substituents may be in either an axial or equatorial position relative to the ring and may change between axial/equatorial.
• the position of the two substituents relative to the ring and each other remains fixed. While either substituent at times may lie in the plane of the ring (equatorial) rather than above or below the plane (axial), one substituent is always above the other.
• a substituent (X j ) which is "below” another substituent (X 2 ) will be identified as being in the alpha ( ⁇ ) configuration and is identified by a broken, dashed or dotted line attachment to the carbon atom, i.e., by the symbol " " or "!.
• the corresponding substituent attached “above” (X 2 ) the other (X j ) is identified as being in the beta ( ⁇ ) configuration and is indicated by an unbroken line attachment to the carbon atom.
• variable substituent when a variable substituent is bivalent, the valences may be taken together or separately or both in the definition of the variable.
• R j is defined to consist of two monovalent variable substituents
• the convention used to define the bivalent variable is of the form " ⁇ -R j ;: ⁇ -R j _ k " or some variant thereof.
• both ⁇ -R j _; and ⁇ -R j _ k are attached to the carbon atom to give -C( ⁇ -R j _ : X ⁇ -R j _ k )-.
• the two monovalent variable substituents are ⁇ -R 6 _ 1 : ⁇ -R 6 _ 2 , •••• ⁇ -R 6 -9 : ⁇ - R 6- ⁇ ⁇ ' etc > g ivin ⁇ -C( ⁇ -R 6 . 1 )( ⁇ -R 6 . 2 )- -C( ⁇ -R 6 .
• bivalent variable may be defined as two separate monovalent variable substituents
• two separate monovalent variable substituents may be defined to be taken together to form a bivalent variable.
• R- and R j may be defined to be taken together to form (1) a second bond between C- and C 2 or (2) a bivalent group such as oxa (-0-) and the formula thereby describes an epoxide.
• the carbon atom content of variable substituents is indicated in one of two ways.
• the first method uses a prefix to the entire name of the variable such as "C j -C 4 ", where both "1" and "4" are integers representing the minimum and maximum number of carbon atoms in the variable.
• the prefix is separated from the variable by a space.
• C r C 4 alkyl represents alkyl of 1 through 4 carbon atoms, (including isomeric forms thereof unless an express indication to the contrary is given). Whenever this single prefix is given, the prefix indicates the entire carbon atom content of the variable being defined.
• C 2 -C 4 alkoxycarbonyl describes a group CH 3 -(CH 2 ) n -0-CO- where n is zero, one or two.
• the carbon atom content of only each portion of the definition is indicated separately by enclosing the "C j -C j " designation in parentheses and placing it immediately (no intervening space) before the portion of the definition being defined.
• this optional conven ⁇ tion (C j -C 3 )alkoxycarbonyl has the same meaning as C 2 -C 4 alkoxycarbonyl because the "C j -C 3 " refers only to the carbon atom content of the alkoxy group.
• C 2 -C 6 alkoxyalkyl and (C I -C 3 )alkoxy(C 1 -C 3 )alkyl define alkoxyalkyl groups containing from 2 to 6 carbon atoms, the two definitions differ since the former definition allows either the alkoxy or alkyl portion alone to contain 4 or 5 carbon atoms while the latter definition limits either of these groups to 3 carbon atoms.
• Chromatography refers to medium pressure chromatography on silica gel.
• THF refers to tetrahydrofuran.
• Saline refers to an aqueous saturated sodium chloride solution.
• NMR nuclear (proton) magnetic resonance spectroscopy, chemical shifts are reported in ppm ( ⁇ ) downfield from tetramethylsilane.
• IR refers to infrared spectroscopy.
• - ⁇ refers to phenyl (C 6 H 5 ).
• MS refers to mass spectrometry expressed as m/e or mass/charge unit.
• [M + H] + refers to the positive ion of a parent plus a hydrogen atom.
• El refers to electron impact.
• CI refers to chemical ionization.
• FAB refers to fast atom bombardment.
• Ether refers to diethyl ether.
• Halo refers to a halogen atom (-C1, -Br, -F or -I).
• compositions and/or substances which are acceptable to the patient from a pharmacological toxicological point of view and to the manufacturing pharmaceutical chemist from a physical/chemical point of view regarding composition, formulation, stability, patient acceptance and bioavailability.
• Pyridinyl refers to the pyridyl radical as defined by IUPAC nomenclature. For example,
• HIV refers to HIV-1.
• Treatment refers to inhibition of the HIV virus and will differ depending on the infected individual.
• the pyrimidine-thioalkyl derivatives of Formula IA will delay, or prevent, the onset of symptoms.
• the pyrimidine-thioalkyl derivatives of Formula IA will delay, or prevent, the onset of "full blown AIDS”.
• the pyrimidine-thioalkyl and alkylether derivatives of Formula IA will extend survival time of these individuals.
• Ammo-6-hydroxy-2-mercaptopyrimidine monohydrate (1.61 g, 10.0 mmol) is suspended in 50% ethanol (10 ml), then treated with solid sodium hydroxide (440 mg, 11.0 mmol) and stirred until the solid dissolved.
• 2,6-Difluoro-benzyl bromide (2.17 g, 10.5 mmol) is added and the reaction heated to reflux for 1.5 hrs. After cooling to 22°C, the solid is collected, washed with water, then air dried. The title compound is recrystallized from ethanol, mp 245-246°C.
• ExVCpd #28 4-amino-2-(6,7-difluoro-2-naphthylmethylthio)-6-hydroxypyrimidine 281 -283(d)
• Ex./Cpd #29 4-ammo-2-(2-quinolinylmemylthio)-6-hydroxypyrimidine
• ExVCpd #41 4-a ⁇ u ⁇ o-6-cMoro-2-(3-fluorophenylmemylthio)-pyrimidine 97-99
• ExVCpd #42 4-ammo-6-chloro-2-(3-chlorophenylmethylthio)-pyrimidine 103-105
• ExVCpd #56 4-amino-6-chloro-2-[2-(3-ethoxy)pyridylmethylthio] -pyrimidine 151.5-154
• ExVCpd #57 4-amino-6-chloro-2-(3-pyridylmethylthio)-pyrimidine 159-161
• ExVCpd #60 4-amino-6-chloro-2-(6,7-difluoro-2-naphthylmethylthio)-pyrimidine 125-127
• ExVCpd #61 4-amino-6-chloro-2-(2-quinolinylmethylthio)-pyrimidine 150-152
• ExVCpd #62 4-amino-6-chloro-2-(6-chloro-5-piperonylmethylthio)-pyrimidine 157-159
• ExVCpd #64 4-amino-6-chloro-2-(E-styrylmethylthio)-pyrimidine 117-120
• ExVCpd #65 4-cUoro-2-(2-naphthylmethylthio)-pyrimidine 76-78
• ExVCpd #86 4-mo hoUno-6-chloro-2-(benzyl ⁇ hio)-pyrimidine 119-120
• ExVCpd #87 4-propylarmno-6-cUoro-2-(benzyltWo)-pyrimidine 67-68
• ExVCpd #90 4-amino-5-methyl-6-chloro-2-(2-naphthylmethylthio)-pyrimidine 156
• ExVCpd #91 4-ammo-5-fluoro-6-chloro-2-(2-naphthylmethylthio)-pyrimidine 160
• ExVCpd #105 4-amino-6-chloro-2-(2-naphthylmethyloxy)-pyrimidine 130-131
• ExVCpd #107 4-ammo-6-cMoro-2-(3-bromophenylmemyloxy)-pyrimidine 96-98
• Example 110 Preparation of 4-amino-6-chloro-2-thio-pyrimidine (Cpd #110) 4-ammo-6-cMoro-2-(4-memoxyphenylme ylthio)-pyrimidine (11.0 g, 39.15 mmol; Cpd #93) and trifluoroacetic acid (84 ml) are heated to reflux for 20 hours, then the excess solvent is removed in vacuo. The sample is triturated with chloroform then stirred with ether and filtered. The solid is washed with ether then air dried, mp >320°C.
• ExVCpd #113 4-amino-6-chloro-2-[2-(6-methyl)pyridylmethylthio] -pyrimidine 156-15
• ExVCpd #114 4-amino-6-chloro-2-[2-(4-methyl)pyridylmethylthio]-pyrimidine 192-19
• ExVCpd #115 4-amino-6-chloro-2-[2-(4-emoxy)pyridylmemyltWo]-pyrimidine 181-18
• ExVCpd #116 4-amino-6-chloro-2- [2-(4-thiophenyl)pyridylmethylthio] -pyrimidine 136-13
• ExVCpd #117 4-amino-6-chloro-2-[2-(3-methyl)pyridylmethylthio]-pyrimidine 148-14
• ExVCpd #118 4-ammo-6-cMoro-2-[2-(5-memyl)pyridylmethyltiiio]-pyrimidine 191-19
• ExVCpd #120 4-amino-6-chloro-2-[2-(4-methoxy-6-methyl)-pyridylmethylthio]- pyrimidine 171-17
• ExVCpd #121 4-ammo-6-cUoro-2-[2-(4,6-dimemyl)pyridylmethylthio]-pyrimidine 160-1
• ExVCpd #122 4-amino-6-chloro-2-[2-(4-ethyl)pyridylmethylthio]-pyrimidine 173-1
• ExVCpd #123 4-amino-6-cUoro-2-[2-(4-memoxy)pyridylmemyltMo]-pyrimidine 191-1
• ExVCpd #124 4-am o-6-chloro-2-[2-(4-(2-memylpropyl))pyridylmemylthio]-pyrimidine 156-1
• ExVCpd #125 4-amino-6-chloro-2-[2-(6-chloro-4-methyl)pyridylmethylthio]-pyrimidine 171-1
• ExVCpd #126 4-am o-6-cMoro-2-[2-(4-isopropoxy)pyridylmethylthio]-pyrimidine 168-1
• ExVCpd #127 4-am o-6-cUoro-2-[2-(4,6-dimethyl)pyrimidinylmethylthio] -pyrimidine 180-1
• ExVCpd #130 4-ammo-6-chloro-2-[4-(6-memyl)pyrimidinylmethylthio]-pyrimidine 165-1
• ExVCpd #131 4-amino-6-chloro-2-[2-(4-propyl)pyridylmethylthio]-pyrimidine 161-1
• ExVCpd #133 4-amino-6-cWoro-2-[2-(5-phenyl)pyridylmethylthio]-pyrimidine 1
• ExVCpd #134 4-ammo-6-chloro-2-[2-(4-ethyl)pyridylmemylthio]-pyrimidine 1
• ExVCpd #135 4-amino-6-chloro-2-[2-(4-( ⁇ -hydroxy, ⁇ -methyl)ethyl) pyridyl-methylthio] -pyrimidine 140- 14
• ExVCpd # 138 4-amino-6-chloro-2-[2-(4-cyclopentyl)pyridylmethylthio]-pyrimidine 138-1
• ExVCpd #140 4-amino-6-chloro-2-[2-(4,5-dimethyl)pyridylmethylthio]-pyrimidine 210-2
• ExVCpd #142 4-amino-6-chloro-2-t4-(2,6-dimethyl)pyrimidinylmethylthio]-pyrimidine 132-1
• ExVCpd #143 4-ammo-6-chloro-2-[2-(4-pyrroUdino)pyridylmethylthio]-pyrimidine 20
• ExVCpd #144 4-Amino-6-cWoro-2-[(5-chlorothiophen-2-ylmethyl)thio]pyrimidine 100-1
• ExVCpd #145 4-ammo-6-cWoro-2-[2-(4-(2-butyl))pyridylmemylthio]-pyrimidine 115-1
• ExVCpd #146 4-amino-6-cUoro-2-[2-(4-dimemylam o)pyridylmemyltWo]-pyrimidine 207-2
• ExVCpd #148 4-Amino-6-chloro-2-[(furan-3-ylmethyl)thio]pyrimidine 83-
• ExVCpd #149 4-ammo-6-chloro-5-fluoro-2-[2-(4-chloro)pyridylmethylthio]pyrimidine 1
• ExVCpd #151 4-amino-6-chloro-2-[2-(4-(3-pentyl))pyridylmethylthio]-pyrimidine 144-1
• ExVCpd #152 4-amino-6-chloro-2-[2-(4-acetyl)pyridylmethylthio] -pyrimidine
• ExVCpd #154 4-amino-6-chloro-2-[2-(6-dimethylamino-4-methyl)pyridylmethylthio]- pyrimidine 166-1
• ExVCpd #155 4-ammo-6-cUoro-2-[(lH-inden-3-ylmemyl)thio]pyrimidine NMR: (CDC1 3 ) 7.47, 7.26, 6.54, 6.15, 4.99, 4.34, 3.37
• ExVCpd #158 4-Amino-6-chloro-2-[(benzothiophen-2-ylmethyl)thio]pyrimidine 155-15
• ExVCpd #159 4-Amino-6-chloro-2-[(2 /-l-benzopyran-3-ylmethyl)thio]pyrimidine 110-11
• Example #163 Preparation of 4-amino-6-chloro-2-[2-(4-carboxamido)- pyridylmethylthio] -pyrimidine (Cpd# 163)
• Lithium aluminum hydride (12 mg, 0.32 mmol) is suspended in THF (1 ml) and cooled to 0°C. The slurry is then treated with a solution of 4-amino-6-chloro-2- [2-(4-carbomethoxy)pyridylmethylthio]-pyrimidine (100 mg, 0.32 mmol) in THF (0.5 ml). The solution is allowed to warm to room temperature and stirred for 1 hour. The reaction is quenched wit water (1 drop), 1 N NaOH (1 drop), and water (3 drops) and diluted with ethyl acetate. The reaction is dried with MgS0 4 and concentrated in vacuo. The resultant solid is triturated with ethyl acetate, mp 117-118 °C.
• ExVCpd #166 4-amino-5-bromo-6-chloro-2-[2-(4-isopropyl)pyridylmethylthio]-pyrimidine 146-14
• ExVCpd #168 4-Amino-6-cMoro-2-[(2,3-dmydrobenzofuran-5-ylmethyl)thio]pyrimidine 153
• ExVCpd #169 4-Amino-6-chloro-2-[(5-phenylisoxazol-3-ylmethyl)thio]pyrimidine 217-21
• ExVCpd #170 4-Am o-6-cWoro-2-[(2,3-dihydrobenzofuran-2-ylme yl)tMo]pyrimidine 105-1
• ExVCpd #171 4-Amino-6-chloro-2-[[(3,4-dihydro-l-na ⁇ hthalen-2-yl)methyl]thio]- pyrimidine 104-1
• ExVCpd #174 4-Ammo-6-chloro-2-[(5-methylisoxazol-3-ylmethyl)thio]pyrirnidine 177-1
• ExVCpd #175 4-Armno-6-chloro-2-[(5-methylpyrazin-2-ylmethyl)thio]pyrimidine 154-15
• ExVCpd #176 4-Amino-6-cWoro-2-[(l-memylimidazol-2-ylmethyl)thio]pyrimidine 178-18
• ExVCpd #177 4-Amino-6-chloro-2-[(3-methylpyrazin-2-ylmethyl)thio]pyrimidine 162-16
• ExVCpd #178 4-Amino-6-chloro-2-[(quinolin-6-ylmethyl)thio]pyrin ⁇ idine 186-188 (
• ExVCpd #182 4-Amino-6-chloro-2-[(isoquinolin-3-ylmethyl)thio]pyrimidine >210
• ExVCpd #183 4-Aj]iino-6-chloro-2-[(quinolin-5-ylmethyl)thio]pyrimidine 190 (
• ExVCpd #184 4-Amino-6-chloro-2-[(quinolin-7-ylmethyl)thio]pyrimidine 195 (
• ExVCpd #186 4-Amino-6-chloro-2-[(piperon-5-ylmethyl)thio]pyrimidine 148-15
• ExVCpd #187 4-Amino-6-chloro-2-[[(3,4-dihydro-l-naphthalenyl)methyl]thio]pyrimidine 127-13
• ExVCpd #188 4-am o-6-chloro-2[2-(5-carbomethyoxy)pyridylmethylthio]pyrimidine 200
• ExVCpd #189 4-amino-6-chloro-2[2-(4-cyclohexyl)pyridylmethylthio]pyrimidine 134
• Example IC50 Example IC50
• Example IC50 Example IC50
• Example IC50 Example IC50
• Example IC50 Example IC50
• Example IC50 Example IC50

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• 1994
  • 1994-11-09 WO PCT/US1994/012713 patent/WO1995013267A1/en not_active Application Discontinuation
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