WO1995001987A1 - Procede de synthese d'acides nucleiques sur support solide et comoposes utiles notamment comme support solide dans ledit procede - Google Patents
Procede de synthese d'acides nucleiques sur support solide et comoposes utiles notamment comme support solide dans ledit procede Download PDFInfo
- Publication number
- WO1995001987A1 WO1995001987A1 PCT/FR1994/000842 FR9400842W WO9501987A1 WO 1995001987 A1 WO1995001987 A1 WO 1995001987A1 FR 9400842 W FR9400842 W FR 9400842W WO 9501987 A1 WO9501987 A1 WO 9501987A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- group
- solid support
- nucleotide
- synthesis
- support
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Definitions
- the present invention relates to a process for the synthesis of nucleic acids on a solid support.
- the present invention also relates to a solid support which is useful, in particular in biotechnology and particularly in the process for the synthesis of nucleic acids according to the invention.
- the present invention finally relates to a process for the preparation of said solid support.
- the synthesis of nucleic acids on a solid support is particularly used in automated syntheses of DNA or RNA oligonucleotides.
- nucleic acids means deoxyribonucleic acids or ribonucleic acids, or, more generally, polynucleotides or oligonucleotides where the bases, inter-nucleotide phosphate bonds, or even the ribose rings of the bases, can be modified. chemically in a known manner. They may in particular be oligonucleotides of ⁇ or ⁇ homogeneities, oligonucleotides of internucleotide link of the phosphorothioate or methyl phosphonate type. or even oligothionucleotides.
- the first step in a process for synthesizing nucleic acids, on a solid support consists in attaching the first nucleoside of the desired sequence to a solid support, traditionally made up of glass beads with controlled porosity (CPG) or, more generally, of a functionalized mineral or organic polymer.
- CPG controlled porosity
- the techniques currently used involve the use of eight different reagents as solid supports, consisting of a functionalized inorganic or organic polymer, linked to a nucleoside A, T, C, G, or U, depending on whether the sequence to be prepared comprises as first deoxyribo- or ribo nucleotide A, T, C, G, or U.
- the manufacturers also supply reactors where one of these nucleosides has been previously attached to the support. Depending on whether the sequence begins with A, T, C, G, or U, the appropriate reactor is therefore chosen. The elongation of this first nucleoside is then done in the direction 3 '->5', or 5 '->3', thanks to coupling reagents.
- a synthesis cycle that is to say the coupling between two nucleotides, comprises at least three steps: (1) deprotection of the OH function in 5 ′ or 3 ′ of a first nucleotide, in particular detritylation, (2 ) activation of said OH function, 5 'or 3' of this first nucleotide and condensation with the 3 ′ or 5 ′ end respectively of a second nucleotide, and finally (3) oxidation of the phosphite group of the internucleotide link obtained in phosphate.
- the synthesis of the oligonucleotide is preferably done in the 3 '-> 5' direction.
- the starting material is a nucleoside protected in 5 OH and attached to the support by the 3 ′ end of the deoxyribose or ribose cycle.
- the nucleotides which are subsequently added are in the form of a 5 'protected derivative and whose 3' hydroxyl has a substituted phosphite or phosphate group.
- the phosphoramidite method described in particular in EP 61746 and US 4,458,066, is today one of the methods of choice, because it allows coupling yields to be achieved high (greater than 98%).
- the 3 'hydroxyl therefore has a phosphoramidite group (see FIG. 1).
- the phosphoramidite group makes the phosphorus atom more susceptible to attack by a primary hydroxyl function, like that in 5 ′ of the nucleosides or detritylated growing chains. (e) s.
- the deprotected 5 'hydroxyl function becomes sufficiently nucleophilic to react with the phosphoramidite group of the second nucleotide.
- Solid phase DNA and RNA syntheses show great homologies.
- the monomers and the supports are different but the instrumentation and the reagents are identical.
- oligonucleotides obtained at the end of the synthesis cycles must be detached from the support and the protective functions must be eliminated.
- the cleavage of the support, the deprotection of the bases and the elimination of the group linked to phosphorus are carried out simultaneously in an ammonia solution.
- ethanol makes it possible to dissolve the 2'O-silyl-oligoribonucleotides and to minimize desilylation, the native RNA not being stable under basic conditions.
- the ammonia / ethanol solution containing the oligoribonucleotide which has passed into the liquid phase is then separated from the glass support and evaporated.
- TBAF tetrabutylammonium fluoride
- TEAA triethyl ammonium acetate
- the solid support must react selectively with the functionalized 3 ′ end of the nucleotide, in particular of the phosphoramidite, H-phosphonate, phosphotriester, phosphodiester, phosphite type, or with any other monomeric reagent according to the synthesis method used;
- the support-oligonucleotide bond must be capable of being hydrolyzed at the end of the synthesis under the conditions of the deprotection step of the oligonucleotide, and
- the covalent link between the support and the oligonucleotide must be such that, when it is dropped, the oligonucleotide released is of the native type, that is to say that the 3 'terminal hydroxyl function is free or does not carry a residue from synthesis.
- These supports can consist of organic polymers such as polystyrene (Nucleic A. Res. 1980, volume 8), polyacrylamide acryloylmorpholide, polydimethylacrylamide polymerized on kieselbow (Nucleic Ac. Res. 9 (7) 1691 (1980)).
- these supports have significant flaws: they are not universal and can only be used in oligonucleotide synthesis after prior preparation of their corresponding nucleoside derivatives, for example, CPG-A, CPG-G, CPG- T, CPG-C or CPG-dA, CPG- dG, CPG-dU, CPG-dC; the preparation of these derivatives also involves a prior preparation of 3'-p-nitrophenyl-succinate-nucleoside which requires more time and significant expense of reagent.
- the supports currently used are linked to the first ribonucleoside or deoxyribonucleoside of the sequence to be synthesized, as mentioned above.
- the operator must therefore choose from supports generally responding to a formula as follows:
- - A is a hydrogen atom (deoxyribonucleoside) or an optionally protected hydroxyl group (ribonucleoside),
- - B is a purine or pirimidic base whose exocyclic amide function is possibly protected.
- These protective agents generally benzoyl or isobutyryl, also help in their solubilization in the organic solvents used during the synthesis,
- C is the usual transient protective group for the terminal 5 ′ function, in general of the trityl type such as dimethoxytrityl,
- - P is the solid support constituted by an inorganic or organic polymer linked directly to the 3 'end, optionally substituted by a divalent hydrocarbon radical linked by an ester link 3' to the nucleoside.
- An object of the present invention is to provide a method for synthesizing oligonucleotides in solid phase, more particularly a method in automatic synthesis, in which a so-called “universal” support is used.
- the term “universal support” is understood here to mean a solid support which can be used whatever the first nucleotide of the RNA or the DNA to be synthesized, whatever the type of monomer reagent used during the synthesis, that is to say whatever the type of substitution on the phosphate group in 3 ′, or in 5 ′ depending on whether the synthesis is carried out in the 5 ′ -> 3 ′ or 3 ′ -> direction 5 '.
- Another object of the present invention is to be able to use this "universal support" in a process involving the same reaction conditions as automated solid phase syntheses.
- an object of the present invention is that the monomer reagent used to hook the first nucleotide on the solid support is a monomer reagent identical to the monomer reagent used to hook the other nucleotides of the sequence during the synthesis, in particular as regards 5 'and 3' protection.
- Another object is also that the solid support conforms to the four characteristics mentioned above.
- the first nucleotide which is introduced comprises a phosphate group in 3 ′ or 5 ′ which must be able, after cleavage between the support and the oligonucleotide under the usual deprotection conditions in basic medium, at the end of synthesis, release a 3 'or 5' OH end, as the case may be.
- the group can be coupled to a 3 ′ or 5 ′ end protected from the monomer reactants, under the same conditions as the 3 ′ or 5 ′ end of the terminal nucleotide of the chain already synthesized is coupled with the 5 ′ end respectively or 3 'of the next monomer reagent to be hung, and
- the "universality" of the supports in solid phase according to the present invention is obtained, thanks to a functionalization of the mineral or organic polymer with a hydrocarbon radical comprising groups of the glycol type in which an OH group and a nucleophilic group are in the vicinal position. , that is to say on two adjacent carbons, at the end of the hydrocarbon radical, these two carbons possibly being optionally substituted by inert groups.
- inert group is understood here to mean a group which does not react under the conditions encountered during the various stages of the synthesis on solid support of nucleic acids according to the invention.
- the present invention therefore relates to a process for the preparation of nucleic acids by synthesis on a solid support, characterized in that a mineral or organic polymer linked by a hydrocarbon radical divalent to an epoxide group or a group of glycol type, the latter consisting of two adjacent saturated carbons on which are respectively substituted an OH group and a nucleophilic group.
- the first nucleotide is attached to the solid support under the same conditions and with the same monomer reagent as for the condensation of the second nucleotide with the first nucleotide linked to the support, which can be the conventional monomer conditions and reagents used during the synthesis of nucleic acids on a solid support, said first nucleotide corresponding to the first nucleotide of the sequence of said nucleic acid.
- the method of the invention comprises the following steps:
- step 2 2) oxidation or sulfurization of the phosphite-type intemucleotide link obtained in step 1) into a phosphate link respectively. 3) deprotection of the 5'-0 or 3'-0 end of the product obtained in step 2);
- the method can include the following steps of:
- step 4) condensation of the 5 'OH or 3' OH group of the product obtained in step 3) with the phosphate, phosphorothioate or phosphite group optionally substituted in the 3 'or 5' position of a nucleotide monomer reagent protected in 5'-0 or 3'-O respectively, using said coupling agent, under the same conditions as step 1);
- the method according to the invention comprises a final step of detaching the nucleic acid from the support and removing the protective groups from the bases and, where appropriate, from the 2'-O positions of the nucleic acid.
- the solid support is already linked to a first nucleoside corresponding to the first nucleotide of the sequence to be prepared, before the start of the synthesis cycles, said support generally comprises a protection 5 'or 3' of said nucleoside.
- the synthesis cycle begins with a deprotection step in an acid medium, in general a detrylylation with TFA, DCA or TCA in dichloromethane.
- the process can also start with a deprotection step and it is then possible to use as initial solid support a support according to the invention comprising an epoxy group.
- the method according to the invention then comprises a prior step of opening said epoxide group from said solid support, in an anhydrous and acid medium, under the usual conditions for deprotection of the OH groups in 5 ′ or 3 ′ to give said group of the glycol type of the solid support.
- the subject of the present invention is also compounds of the following formulas and their use as a solid support in a process for the synthesis of nucleic acids according to the invention:
- R 1 , R ' 1 , R " 1 , R 2 and R' 2 represents a mineral or organic polymer - P or a hydrocarbon radical substituted by a mineral or organic polymer
- the others represent H, or an inert group such as an alkyl group optionally substituted, in particular by one or more halogen (s),
- - Nu is a nucleophilic group such as NH 2 , -O-Alk, -NHAlk, -N (Alk) 2 ,
- Nu is -N (Alk) 2 , -NHAc, -O-Ac, -SAc and a halogen.
- said solid support uses one of the formulas:
- R 1 and R 2 or R ' 1 and R' 2 together form a cycle, in particular a heterocycle, on which the polymer is substituted.
- said solid support consists of a compound (I), (Ia), (Ib), (II), (IIa), (IIb) or (I ') and (I'b) according to the invention.
- said nucleotide monomer reagent corresponds to the formula:
- - A represents H or an optionally protected hydroxyl group
- - B is a purine or pyrimidine base whose exocyclic amine function is optionally protected
- - C is a conventional protecting group for the 5'-OH function
- R 3 represents H and R 4 represents a negatively charged oxygen atom
- R 3 is an oxygen atom and R 4 represents either an oxygen atom or an oxygen atom carrying a protective group, and
- R 3 is an oxygen atom carrying a protective group and R 4 is either a halogen or a disubstituted amino group.
- R 3 is an oxygen atom and R 4 is an oxygen atom, it is then the so-called phosphodiesters method, when R 4 is an oxygen atom carrying a protective group, this is then the so-called phosphotriester method.
- R 3 is a hydrogen atom and R 4 is a hydrogen atom and R 4 is a negatively charged oxygen atom, this is then the so-called H-phosphosphonate method
- - When x is equal to O, R 3 is an oxygen atom carrying a protective group and R 4 is either a halogen, it is then the so-called phosphite method and, when R 4 is a leaving group of the disubstituted amino type, this is then the so-called phosphoramidite method.
- the support reagents of formula I, I ′ and II according to the present invention react with the usual monomer reagents III, under the usual conditions of condensation in an acid medium in the methods of synthesis of nucleic acids on solid support, according to the following scheme :
- D represents an oligonucleotide
- the other parameters have the values given above.
- the polymer can be found in R 2 , that is to say substituted on the phosphate ring or in R 1 .
- polymer mention is made of materials consisting of glass microbeads or microfibers, in particular porous, silica, metal oxides, or organic polymers, in particular cellulose, or optionally substituted polystyrene.
- the polymer is an inorganic polymer based on glass or silica, in particular a silica gel.
- the compounds of formulas (I), (V) and (II) can be prepared by methods known to those skilled in the art and using available reagents.
- the compounds of formula (I), (I ') or (II) can, for example, be prepared from a polymer functionalized with a COOH group or
- R 1 P - R -.
- X - R can just as easily be substituted for R ' 1 .
- the compounds of formulas (I ') and (II) can also be prepared according to this same type of reaction from P-NH 2 and a compound where X - R is substituted for R 1 , R' 1 or R " 1 in said formulas.
- the compounds of formulas (I ') can also be prepared from P- R 1 - NH 2 and
- the solid support When the solid support is represented by formula (I), it can also be prepared by an opening reaction of the epoxide ring of formula in an anhydrous, acidic or basic medium according to a substitution mechanism respectively SN 1 or SN 2 , in the presence of HNu in the medium where Nu represents said nucleophilic group.
- the solid support When the solid support is represented by formula (II) with P included in R 1 , it can be prepared from a polymer functionalized by a carboxyl function (this type of polymer is commercially available) according to the following scheme:
- the Si - OH groups thereof can be reacted with compounds
- R ' is such that P- Si - R' - represents R 1 under conditions known to those skilled in the art, for example at 50 ° C as illustrated in Example 1, where the compound (I) is obtained by treating the surface of the solid phase with 10% glycidiloxypropyl-timethoxysilane in a solution of acetonitrile or with another reagent containing an epoxide, followed by an opening of the epoxide cycle under controlled conditions.
- CPG solid support
- the support is compatible with all the synthesizers; - the deprotection step in a DNA or RNA synthesis process is carried out under the same conditions as for a conventional support; there is no additional step for the user of the universal support in a DNA or RNA synthesis process;
- the support can be used for the manufacture of oligonucleotides modified at the 3 'terminal end by directly using, in the first cycle, the monomers corresponding to the nature of the desired modification;
- the universal support makes it possible to design a mult ' .reactor synthesis system considerably simplified by the independence of each reactor vis-à-vis the sequence to be synthesized.
- P- is a material consisting of microbeads or microfibers of glass, silica, metal oxides, cellulose, or organic polymers such as polystyrene, and in which:
- k is an integer which can vary from 1 to 20
- 1 is an integer that can vary from 0 to 1
- n is an integer that can vary from 0 to 1
- n is an integer that can vary from 0 to 100
- X represents -H, -N (Alk) 2 , -NHAcyl, -OAcyl, -SAcyl, -Hal,
- y represents -H, -or, -O-, -NHAlk, -S-,
- a synthesis cycle corresponding to the addition of a nucleotide, also comprises three stages: unblocking, coupling and oxidation.
- the deblocking step or detritylation
- the 5 'hydroxyl terminal of the oligonucleotide in the course of synthesis protected by the Dmtr group is deprotected under the action of trichloraeetic acid (TCA).
- TCA trichloraeetic acid
- the posphoramidite grouping of the monomer reagent is activated by tetrazole and reacts with the free terminal 5 'hydroxyl to form an intemucleotide bond of phosphite type.
- the unstable (trivalent) phosphite is then oxidized to phosphotriester (pentavalent) in the presence of water and iodine.
- This fourth step of "capping" consists of acetylation of the hydroxyls in 5 'with acetic anhydride and N-methylimidazole.
- reagents used in the different stages are as follows:
- R 3 - (CH 2 ) 2 - C ⁇ N
- Diagram 1 represents the decryptylation.
- the number of oxy groups is defined, after the opening of the epoxide ring thanks to the reaction of dimethoxytrityl chloride in pyridine followed by the spectrophotometric measurement of absorption of the trityl cation in the mixture of perchloric acid and ethanol at 495 nm . A capacity of 50-100 micromoles is obtained for 1 g of support.
- the reactor is filled with 1 mg of support, obtained in Example 1, and the oligonucleotide d (ATGC) is synthesized by the standard method with phosphoramidites recalled above with a first step in the conditions of decryptylation which opens the epoxy cycle.
- ATGC oligonucleotide d
- Poligo-CPG is heated for one hour at 100 ° C. in 30 microliters of concentrated aqueous ammonia solution.
- the oligonucleotide is released, the last nucleotide of which is protected in 5 'below abbreviated ON-trityl using HPLC in a column with reversed phase. About 90% of ON-trityl oligonucleotide is obtained.
- Example 2 The synthesis of Example 2 was carried out with a synthesis of (AGTC) by the H-Phosphonates method.
- the activating agent used is either adamantoyl chloride or pivaloyl chloride,
- RNA oligoribonucleotides
- a membrane is treated in the form of a fiberglass disc (0 4.7 cm, 1 g, f. WATMAN) ® as in Example 1.
- a support with a capacity of 20 ⁇ moles of oxy groups is obtained for 1 g of support.
- Example 4 From the disc obtained in Example 4, a disc (0 4 mm, 1 mg) is cut and the synthesis, processing and deraching of the oligonucleotides d (AGTC) is carried out as in Example 3.
- a support with a capacity of 50-100 ⁇ mol of oxy groups is obtained for 1 g of support.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Saccharide Compounds (AREA)
- Catalysts (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Steroid Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Description
Claims
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002166765A CA2166765A1 (fr) | 1993-07-09 | 1994-07-07 | Procede de synthese d'acides nucleiques sur support solide et composes utiles notamment comme support solide dans ledit procede |
AU72309/94A AU696421B2 (en) | 1993-07-09 | 1994-07-07 | Process for the synthesis of nucleic acids on a solid support and compounds which are useful in particular as solid supports in the said process |
KR1019960700059A KR960703937A (ko) | 1993-07-09 | 1994-07-07 | 고체 지지체상에서 핵산을 합성하는 방법 및 이 방법에서 고체 지지체로서 유용한 화합물(process for solid support nucleic acid synthesis and compounds useful as solid supports in said process) |
EP94921699A EP0707592B1 (fr) | 1993-07-09 | 1994-07-07 | Procede de synthese d'acides nucleiques sur support solide et comoposes utiles notamment comme support solide dans ledit procede |
JP7503866A JPH08512306A (ja) | 1993-07-09 | 1994-07-07 | 固体の支持体上で核酸を合成する方法および特に前記方法における固体の支持体として有用である化合物 |
DE69405396T DE69405396T2 (de) | 1993-07-09 | 1994-07-07 | Verfahren zur synthese von nukleinsäuren auf einem festträger und verbindungen verwendbar als festträger in diesem verfahren |
GR970403065T GR3025420T3 (en) | 1993-07-09 | 1997-11-19 | Process for solid support nucleic acid synthesis and compounds useful as solid supports in said process |
HK98101580A HK1002731A1 (en) | 1993-07-09 | 1998-02-27 | Process for solid support nucleic acid synthesis and compounds useful as solid supports in said process |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR93/08498 | 1993-07-09 | ||
FR9308498A FR2707296B1 (fr) | 1993-07-09 | 1993-07-09 | Procédé de synthèse d'acides nucléiques sur support solide et composés utiles notamment comme support solide dans ledit procédé. |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08591466 A-371-Of-International | 1996-01-11 | ||
US09/076,956 Division US20020103365A1 (en) | 1993-07-09 | 1998-05-13 | Process for the synthesis of nucleic acids on a solid support and compounds which are useful in particular as solid supports in the said process |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1995001987A1 true WO1995001987A1 (fr) | 1995-01-19 |
Family
ID=9449129
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FR1994/000842 WO1995001987A1 (fr) | 1993-07-09 | 1994-07-07 | Procede de synthese d'acides nucleiques sur support solide et comoposes utiles notamment comme support solide dans ledit procede |
Country Status (14)
Country | Link |
---|---|
EP (1) | EP0707592B1 (fr) |
JP (1) | JPH08512306A (fr) |
KR (1) | KR960703937A (fr) |
AT (1) | ATE157668T1 (fr) |
AU (1) | AU696421B2 (fr) |
CA (1) | CA2166765A1 (fr) |
DE (1) | DE69405396T2 (fr) |
DK (1) | DK0707592T3 (fr) |
ES (1) | ES2109005T3 (fr) |
FR (1) | FR2707296B1 (fr) |
GR (1) | GR3025420T3 (fr) |
HK (1) | HK1002731A1 (fr) |
TW (1) | TW397839B (fr) |
WO (1) | WO1995001987A1 (fr) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997040458A2 (fr) * | 1996-04-22 | 1997-10-30 | Beckman Instruments, Inc. | Supports solides universels et leurs procedes d'utilisation |
EP1006121A1 (fr) * | 1998-12-02 | 2000-06-07 | Council of Scientific and Industrial Research | Support polymère universel pour la synthèse des oligonucléotides |
WO2000053578A1 (fr) * | 1999-03-11 | 2000-09-14 | Kuraray Co., Ltd. | Derives de la vitamine d et leur procede de preparation |
US6590092B1 (en) | 1998-05-19 | 2003-07-08 | Nam Q. Ngo | Process for preparing a “universal support” and the reagents used for generating such support |
US6623703B1 (en) * | 1993-12-16 | 2003-09-23 | Proligo, Llc | Process for preparing polynucleotides on a solid support and apparatus permitting its implementation |
US6921669B2 (en) * | 2000-05-16 | 2005-07-26 | Micronas Holding Gmbh | Linker system for activating surfaces for bioconjugation |
US7553958B2 (en) | 2001-03-14 | 2009-06-30 | The Regents Of The University Of Michigan | Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports |
US10781175B2 (en) | 2016-07-15 | 2020-09-22 | Am Chemicals Llc | Solid supports and phosphoramidite building blocks for oligonucleotide conjugates |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU779411B2 (en) | 1999-02-12 | 2005-01-20 | Serono Genetics Institute S.A. | Biallelic markers derived from genomic regions carrying genes involved in arachidonic acid metabolism |
EP1925672A1 (fr) | 2001-06-22 | 2008-05-28 | Syngeta Participations AG | Polynucléotides et polypeptides sensibles au stress abiotique |
US7858297B2 (en) | 2001-12-18 | 2010-12-28 | Centre National De La Recherche Scientifique Cnrs | Chemokine-binding protein and methods of use |
US7572886B2 (en) | 2001-12-18 | 2009-08-11 | Centre National De La Recherche Scientifique | Death associated proteins, and THAP1 and PAR4 pathways in apoptosis control |
EP1485408A2 (fr) | 2002-03-13 | 2004-12-15 | Bayer CropScience S.A. | Mutagenese de champignons aspergillus et de genes essentiels a leur croissance |
FR2900415B1 (fr) | 2006-04-28 | 2010-08-13 | Agronomique Inst Nat Rech | Systeme genetique pour le controle du developpement du type floral d'une plante dicotyledone, et mise en oeuvre dans les procedes de detection et de selection |
FR2954349A1 (fr) | 2009-12-22 | 2011-06-24 | Agronomique Inst Nat Rech | Sulfatase modifiant selectivement les glycosaminoglycanes |
US10865445B2 (en) | 2010-08-18 | 2020-12-15 | Fred Hutchinson Cancer Research Center | Methods for alleviating facioscapulohumeral dystrophy (FSHD) by N siRNA molecule inhibiting the expression of DUX4-FL |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1985001051A1 (fr) * | 1983-09-02 | 1985-03-14 | Molecular Biosystems, Inc. | Systeme de support polymere d'oligonucleotides |
WO1986007361A1 (fr) * | 1985-06-13 | 1986-12-18 | Amgen | Procede de derivation de polynucleotides |
WO1991000868A1 (fr) * | 1989-07-07 | 1991-01-24 | National Research Development Corporation | Polynucleotides immobilises |
WO1991008307A1 (fr) * | 1989-12-04 | 1991-06-13 | Microprobe Corporation | Capture amelioree d'acide nucleique cible faisant appel a des oligonucleotides attaches de façon covalente a des polymeres |
WO1992007882A1 (fr) * | 1990-10-26 | 1992-05-14 | Genta Incorporated | Reactifs polymeres organiques non aromatiques pour la synthese en phase solide d'oligomeres |
WO1992007864A1 (fr) * | 1990-10-26 | 1992-05-14 | Genta Incorporated | Procede ameliore de synthese d'oligomeres |
-
1993
- 1993-07-09 FR FR9308498A patent/FR2707296B1/fr not_active Expired - Fee Related
-
1994
- 1994-07-07 DE DE69405396T patent/DE69405396T2/de not_active Expired - Lifetime
- 1994-07-07 EP EP94921699A patent/EP0707592B1/fr not_active Expired - Lifetime
- 1994-07-07 JP JP7503866A patent/JPH08512306A/ja active Pending
- 1994-07-07 ES ES94921699T patent/ES2109005T3/es not_active Expired - Lifetime
- 1994-07-07 AU AU72309/94A patent/AU696421B2/en not_active Expired
- 1994-07-07 AT AT94921699T patent/ATE157668T1/de not_active IP Right Cessation
- 1994-07-07 CA CA002166765A patent/CA2166765A1/fr not_active Abandoned
- 1994-07-07 DK DK94921699.8T patent/DK0707592T3/da active
- 1994-07-07 WO PCT/FR1994/000842 patent/WO1995001987A1/fr active IP Right Grant
- 1994-07-07 KR KR1019960700059A patent/KR960703937A/ko not_active Application Discontinuation
- 1994-11-18 TW TW083110699A patent/TW397839B/zh not_active IP Right Cessation
-
1997
- 1997-11-19 GR GR970403065T patent/GR3025420T3/el unknown
-
1998
- 1998-02-27 HK HK98101580A patent/HK1002731A1/xx not_active IP Right Cessation
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1985001051A1 (fr) * | 1983-09-02 | 1985-03-14 | Molecular Biosystems, Inc. | Systeme de support polymere d'oligonucleotides |
WO1986007361A1 (fr) * | 1985-06-13 | 1986-12-18 | Amgen | Procede de derivation de polynucleotides |
WO1991000868A1 (fr) * | 1989-07-07 | 1991-01-24 | National Research Development Corporation | Polynucleotides immobilises |
WO1991008307A1 (fr) * | 1989-12-04 | 1991-06-13 | Microprobe Corporation | Capture amelioree d'acide nucleique cible faisant appel a des oligonucleotides attaches de façon covalente a des polymeres |
WO1992007882A1 (fr) * | 1990-10-26 | 1992-05-14 | Genta Incorporated | Reactifs polymeres organiques non aromatiques pour la synthese en phase solide d'oligomeres |
WO1992007864A1 (fr) * | 1990-10-26 | 1992-05-14 | Genta Incorporated | Procede ameliore de synthese d'oligomeres |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6623703B1 (en) * | 1993-12-16 | 2003-09-23 | Proligo, Llc | Process for preparing polynucleotides on a solid support and apparatus permitting its implementation |
US6825339B2 (en) | 1993-12-16 | 2004-11-30 | Proligo, Llc | Apparatus for preparing polynucleotides on a solid support |
WO1997040458A2 (fr) * | 1996-04-22 | 1997-10-30 | Beckman Instruments, Inc. | Supports solides universels et leurs procedes d'utilisation |
WO1997040458A3 (fr) * | 1996-04-22 | 1997-12-04 | Beckman Instruments Inc | Supports solides universels et leurs procedes d'utilisation |
US6590092B1 (en) | 1998-05-19 | 2003-07-08 | Nam Q. Ngo | Process for preparing a “universal support” and the reagents used for generating such support |
EP1006121A1 (fr) * | 1998-12-02 | 2000-06-07 | Council of Scientific and Industrial Research | Support polymère universel pour la synthèse des oligonucléotides |
WO2000053578A1 (fr) * | 1999-03-11 | 2000-09-14 | Kuraray Co., Ltd. | Derives de la vitamine d et leur procede de preparation |
US6921669B2 (en) * | 2000-05-16 | 2005-07-26 | Micronas Holding Gmbh | Linker system for activating surfaces for bioconjugation |
US7553958B2 (en) | 2001-03-14 | 2009-06-30 | The Regents Of The University Of Michigan | Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports |
EP2048149A3 (fr) * | 2001-03-14 | 2009-07-01 | The Regents of the University of Michigan | Liens et agents de co-couplage pour optimiser la synthèse d'oligonucléotides et leur purification sur un support solide |
US7807807B2 (en) | 2001-03-14 | 2010-10-05 | The Regents Of The University Of Michigan | Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports |
US8053187B2 (en) | 2001-03-14 | 2011-11-08 | The Regents Of The University Of Michigan | Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports |
US8461317B2 (en) | 2001-03-14 | 2013-06-11 | The Regents Of The University Of Michigan | Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports |
US9303055B2 (en) | 2001-03-14 | 2016-04-05 | The Regents Of The University Of Michigan | Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports |
US10781175B2 (en) | 2016-07-15 | 2020-09-22 | Am Chemicals Llc | Solid supports and phosphoramidite building blocks for oligonucleotide conjugates |
US11447451B2 (en) | 2016-07-15 | 2022-09-20 | Am Chemicals Llc | Solid supports and phosphoramidite building blocks for oligonucleotide conjugates |
Also Published As
Publication number | Publication date |
---|---|
DE69405396D1 (de) | 1997-10-09 |
FR2707296B1 (fr) | 1995-09-29 |
AU696421B2 (en) | 1998-09-10 |
DK0707592T3 (da) | 1998-03-30 |
ES2109005T3 (es) | 1998-01-01 |
EP0707592B1 (fr) | 1997-09-03 |
DE69405396T2 (de) | 1998-03-26 |
HK1002731A1 (en) | 1998-09-11 |
EP0707592A1 (fr) | 1996-04-24 |
JPH08512306A (ja) | 1996-12-24 |
ATE157668T1 (de) | 1997-09-15 |
TW397839B (en) | 2000-07-11 |
CA2166765A1 (fr) | 1995-01-19 |
FR2707296A1 (fr) | 1995-01-13 |
GR3025420T3 (en) | 1998-02-27 |
KR960703937A (ko) | 1996-08-31 |
AU7230994A (en) | 1995-02-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0707592B1 (fr) | Procede de synthese d'acides nucleiques sur support solide et comoposes utiles notamment comme support solide dans ledit procede | |
CA1273589A (fr) | Supports, leur preparation et les intermediaires obtenus, leur application a la synthese d'oligonucleotides relies aux supports ainsi obtenus | |
EP0206913B1 (fr) | Nouveaux supports, la préparation de ces supports, les intermédiaires nouveaux obtenus, leur application à la synthèse d'oligonucléotides et les nouveaux nécléosides et oligonucléotides reliés aux supports ainsi obtenus | |
US6166198A (en) | Methods for oligonucleotide synthesis | |
WO1998008858A1 (fr) | Reactifs ameliores et procede de synthese d'oligonucleotides contenant des liaisons internucleosides phosphorodithioate | |
WO1993016095A1 (fr) | Oligothionucleotides | |
JPS6250479B2 (fr) | ||
JP2000500158A (ja) | ユニバーサルな固体支持体およびその使用方法 | |
WO1988004301A1 (fr) | OLIGONUCLEOTIDES alpha | |
EP0607337B1 (fr) | Procede de synthese d'acide ribonucleique (arn) utilisant un nouveau reactif de deprotection | |
JP2000506849A (ja) | オリゴヌクレオチド類似体 | |
RU2111971C1 (ru) | Модифицированные олигодезоксирибонуклеотиды, композиция на их основе и промежуточные соединения | |
JP2004508379A (ja) | オリゴヌクレオチド合成用のシントン | |
WO1997029116A1 (fr) | Phosphoramidites de dinucleotides contenant du soufre | |
EP1474430B1 (fr) | Metallocenes bifonctionnalises, utilisation pour le marquage de molecules biologiques | |
WO1990003381A1 (fr) | Procede de synthese d'oligoribonucleotides alpha et composes utiles dans le procede | |
CN112368292B (zh) | 一种亚磷酰胺类化合物、其制备方法及应用 | |
EP0819134B1 (fr) | Oligonucleotides a liaison covalente transversale, procede de preparation et synthon utile dans le procede | |
US20020103365A1 (en) | Process for the synthesis of nucleic acids on a solid support and compounds which are useful in particular as solid supports in the said process | |
EP0902788B1 (fr) | Derives de nucleosides, et leur utilisation pour la synthese d'oligonucleotides | |
FR2529892A1 (fr) | Nouveau support pour la synthese des polynucleotides, en particulier par la methode triester et procede pour sa preparation | |
US20230203084A1 (en) | Sensitive oligonucleotide synthesis using sulfur-based functions as protecting groups and linkers | |
JPH07165786A (ja) | 5位置換ウリジン類、その製造方法及びその用途 | |
FR2686882A1 (fr) | Oligothionucleotides. | |
FR2612930A1 (fr) | Sondes oligonucleotidiques a |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AU CA JP KR US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2166765 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1994921699 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref document number: 1996 591466 Country of ref document: US Date of ref document: 19960111 Kind code of ref document: A |
|
WWP | Wipo information: published in national office |
Ref document number: 1994921699 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 1994921699 Country of ref document: EP |