WO1992011375A1 - Genetically engineered modification of potato to form amylose-type starch - Google Patents

Genetically engineered modification of potato to form amylose-type starch Download PDF

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Publication number
WO1992011375A1
WO1992011375A1 PCT/SE1991/000891 SE9100891W WO9211375A1 WO 1992011375 A1 WO1992011375 A1 WO 1992011375A1 SE 9100891 W SE9100891 W SE 9100891W WO 9211375 A1 WO9211375 A1 WO 9211375A1
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WO
WIPO (PCT)
Prior art keywords
potato
starch
gene
amylose
antisense
Prior art date
Application number
PCT/SE1991/000891
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English (en)
French (fr)
Inventor
Per Hofvander
Per T. Persson
Anneli Tallberg
Olle Wikström
Original Assignee
Amylogene Hb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amylogene Hb filed Critical Amylogene Hb
Priority to PL91299927A priority Critical patent/PL169859B1/pl
Publication of WO1992011375A1 publication Critical patent/WO1992011375A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Definitions

  • the present invention relates to genetically engi- neered modification of potato, resulting in the formation of an increasing amount of amylose-type starch as compared to amylopectin-type starch in the potato.
  • the genetically engineered modification implies the insertion of a gene fragment into potato, said gene fragment comprising tran- scription start and a part of the gene coding for the for ⁇ mation of branching enzyme (BE gene) in potato, inserted in the antisense direction, together with a tuber-specific promoter.
  • BE gene branching enzyme
  • starch In the potato tuber, starch is the greatest part of the solid matter. About 1/4 to 1/5 of the starch in potato is amylose, while the remainder of the starch is amylo- pectin. These two components of the starch have different fields of application, and therefore the possibility of producing either pure amylose or pure amylopectin is most interesting.
  • the two starch components can be produced from common starch, which requires a number of process steps and, consequently, is expensive and complicated.
  • BE branching enzyme
  • Branching enzyme (BE) in potato is a monomer protein, i.e. it is a single enzyme form.
  • the molecular weight of potato BE varies between 79 and 103 kD, depending on the purifying process used. There are indications that potato BE should consist of several forms, but presumably several forms are degradation products from the actual protein (Vos-Scheperkeuter, 1989; Blennow & Johansson, 1990).
  • the invention comprises a method of sup ⁇ pressing formation of amylopectin-type starch in potato, whereby the potato tubers form a varyingly increased amount of amylose-type starch.
  • amylose Physical modification of the amylose can be effected by e.g. cylinder-drying or extrusion.
  • Example 5 Chemical derivatisation of amylose Amylose is sludged in water to a concentration of 20-50% by weight. The pH is adjusted to 5.0-12.0, and sodium hypochlorite is added so that the end product obtains the desired viscosity. The reaction temperature is set at 20-45°C. When the reaction is completed, the pH is adjusted to 4-8, whereupon the end product is washed and dried. In this manner, oxidised starch is obtained.
  • Example 6 Physical derivatisation of amylose
  • SEQ ID No. 1 Sequenced molecule: genomic DNA Name: Promoter for the GBSS gene from potato Length of sequence: 629 bp

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PCT/SE1991/000891 1990-12-21 1991-12-20 Genetically engineered modification of potato to form amylose-type starch WO1992011375A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL91299927A PL169859B1 (pl) 1990-12-21 1991-12-20 Sposób hamowania tworzenia sie w ziemniakach skrobi typu amylopektyny PL

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9004095A SE467160B (sv) 1990-12-21 1990-12-21 Genetisk foeraendring av potatis foer bildning av staerkelse av amylostyp
SE9004095-7 1990-12-21

Publications (1)

Publication Number Publication Date
WO1992011375A1 true WO1992011375A1 (en) 1992-07-09

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1991/000891 WO1992011375A1 (en) 1990-12-21 1991-12-20 Genetically engineered modification of potato to form amylose-type starch

Country Status (5)

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EP (1) EP0563201A1 ( )
AU (1) AU9109791A ( )
PL (1) PL169859B1 ( )
SE (1) SE467160B ( )
WO (1) WO1992011375A1 ( )

Cited By (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0584809A2 (en) * 1992-08-28 1994-03-02 National Starch and Chemical Investment Holding Corporation Low amylopectin starch
WO1994011520A2 (en) * 1992-11-09 1994-05-26 Zeneca Limited Novel plants and processes for obtaining them
WO1995007355A1 (en) * 1993-09-09 1995-03-16 Institut Für Genbiologische Forschung Berlin Gmbh Combination of dna sequences which enable the formation of modified starch in plant cells and plants, processes for the production of these plants and the modified starch obtainable therefrom
WO1995026407A1 (en) * 1994-03-25 1995-10-05 National Starch And Chemical Investment Holding Corporation Method for producing altered starch from potato plants
WO1995031553A1 (en) * 1994-05-18 1995-11-23 Institut Für Genbiologische Forschung Berlin Gmbh DNA SEQUENCES CODING FOR ENZYMES CAPABLE OF FACILITATING THE SYNTHESIS OF LINEAR α-1,4 GLUCANS IN PLANTS, FUNGI AND MICROORGANISMS
WO1996034968A2 (en) * 1995-05-05 1996-11-07 National Starch And Chemical Investment Holding Corporation Improvements in or relating to plant starch composition
WO1997004113A2 (en) * 1995-07-14 1997-02-06 Danisco A/S Inhibition of gene expression
WO1997004112A2 (en) * 1995-07-14 1997-02-06 Danisco A/S Inhibition of gene expression
WO1997011188A1 (de) * 1995-09-19 1997-03-27 Planttec Biotechnologie Gmbh Pflanzen, die eine modifizierte stärke synthetisieren, verfahren zu ihrer herstellung sowie modifizierte stärke
WO1998020145A2 (en) * 1996-11-05 1998-05-14 National Starch And Chemical Investment Holding Corporation Improvements in or relating to starch content of plants
WO1998037213A1 (en) * 1997-02-21 1998-08-27 Danisco A/S Antisense intron inhibition of starch branching enzyme expression
US5908975A (en) * 1993-11-09 1999-06-01 E. I. Du Pont De Nemours And Company Accumulation of fructans in plants by targeted expression of bacterial levansucrase
US5998701A (en) * 1997-06-04 1999-12-07 Her Majesty The Queen In Right Of Canada As Represented By The Department Of Agriculture Potatoes having improved quality characteristics and methods for their production
DE19836098A1 (de) * 1998-07-31 2000-02-03 Hoechst Schering Agrevo Gmbh Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke
US6066782A (en) * 1993-09-09 2000-05-23 Hoechst Schering Agrevo Gmbh Combination of DNA sequences which enable the formation of modified starch in plant cells and plants, processes for the production of these plants and the modified starch obtainable therefrom
US6143562A (en) * 1995-04-06 2000-11-07 Seminis Vegetable Seeds Carbon-based process for selection of transgenic plant cells
EP1103617A2 (en) * 1991-02-13 2001-05-30 Aventis CropScience GmbH Plasmids containing DNA-sequences that cause changes in the carbohydrate composition in plants, as well as plant cells and plants containing these plasmids
US6521816B1 (en) 1998-11-09 2003-02-18 Planttec Biotechnologie Gmbh Forschung Und Entwicklung Nucleic acid molecules from rice and their use for the production of modified starch
WO2003054024A1 (de) * 2001-12-21 2003-07-03 Bayer Cropscience Gmbh Quellstärken und verfahren zu deren herstellung
WO2003078629A1 (de) 2002-03-20 2003-09-25 Basf Plant Science Gmbh Konstrukte und verfahren zur regulation der genexpression
WO2004005516A1 (en) * 2002-07-09 2004-01-15 Basf Plant Science Gmbh Use of ahas mutant genes as selection marker in potato transformation
US7012171B2 (en) 1989-12-21 2006-03-14 Advanced Technologies Cambridge Limited Modification of plant metabolism
US7186898B1 (en) 1996-12-19 2007-03-06 Bayer Bioscience Gmbh Nucleic acid molecules from maize and their use for the production of modified starch
EP2267138A2 (en) 1998-04-08 2010-12-29 Commonwealth Scientific and Industrial Research Organization Methods and means for obtaining modified phenotypes
US8598332B1 (en) 1998-04-08 2013-12-03 Bayer Cropscience N.V. Methods and means for obtaining modified phenotypes
WO2013184768A1 (en) 2012-06-05 2013-12-12 University Of Georgia Research Foundation, Inc. Compositions and methods of gene silencing in plants
US9029527B2 (en) 1998-03-20 2015-05-12 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs
EP2980220A1 (en) 2005-09-20 2016-02-03 BASF Plant Science GmbH Improved methods controlling gene expression
US9441239B2 (en) 1998-04-08 2016-09-13 Commonwealth Scientific & Industrial Research Organisation Methods and means for obtaining modified phenotypes
US9708621B2 (en) 1999-08-13 2017-07-18 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US9963698B2 (en) 1998-03-20 2018-05-08 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
CN114703208A (zh) * 2022-01-21 2022-07-05 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) 马铃薯StGAPC基因在提高马铃薯淀粉含量上的用途

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE513209C2 (sv) * 1995-11-29 2000-07-31 Lars Rask Förfarande för produktion av transgena potatisar med ökad eller minskad grad av förgrening av amylopektinstärkelse

Citations (2)

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EP0335451A2 (en) * 1988-03-25 1989-10-04 Vereniging Voor Christelijk Wetenschappelijk Onderwijs Genetically engineered plant cells and plants, as well as recombinant DNA suitable therefor
EP0368506A2 (en) * 1988-11-10 1990-05-16 Zeneca Limited ADP glucose-pyrophosphorylase

Patent Citations (2)

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EP0335451A2 (en) * 1988-03-25 1989-10-04 Vereniging Voor Christelijk Wetenschappelijk Onderwijs Genetically engineered plant cells and plants, as well as recombinant DNA suitable therefor
EP0368506A2 (en) * 1988-11-10 1990-05-16 Zeneca Limited ADP glucose-pyrophosphorylase

Non-Patent Citations (2)

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Title
BLENNOW A., JOHANSSON G.: "ISOLATION OF A Q-ENZYME WITH M, 103000 FROM POTATO TUBERS.", PHYTOCHEMISTRY, PERGAMON PRESS, GB, vol. 30., no. 02., 1 January 1991 (1991-01-01), GB, pages 437 - 444., XP000617730, ISSN: 0031-9422, DOI: 10.1016/0031-9422(91)83700-U *
VOS-SCHEPERKEUTER G. H., ET AL.: "IMMUNOLOGICAL COMPARISON OF THE STARCH BRANCHING ENZYMES FROM POTATO TUBERS AND MAIZE KERNELS.", PLANT PHYSIOLOGY., AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD., US, vol. 90., 1 January 1989 (1989-01-01), US, pages 75 - 84., XP000617628, ISSN: 0032-0889 *

Cited By (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7012171B2 (en) 1989-12-21 2006-03-14 Advanced Technologies Cambridge Limited Modification of plant metabolism
EP1103617A2 (en) * 1991-02-13 2001-05-30 Aventis CropScience GmbH Plasmids containing DNA-sequences that cause changes in the carbohydrate composition in plants, as well as plant cells and plants containing these plasmids
EP1103617A3 (en) * 1991-02-13 2001-11-28 Aventis CropScience GmbH Plasmids containing DNA-sequences that cause changes in the carbohydrate composition in plants, as well as plant cells and plants containing these plasmids
EP0584809B1 (en) * 1992-08-28 1997-12-29 National Starch and Chemical Investment Holding Corporation Low amylopectin starch
EP0584809A2 (en) * 1992-08-28 1994-03-02 National Starch and Chemical Investment Holding Corporation Low amylopectin starch
WO1994011520A2 (en) * 1992-11-09 1994-05-26 Zeneca Limited Novel plants and processes for obtaining them
WO1994011520A3 (en) * 1992-11-09 1994-08-04 Zeneca Ltd Novel plants and processes for obtaining them
WO1995007355A1 (en) * 1993-09-09 1995-03-16 Institut Für Genbiologische Forschung Berlin Gmbh Combination of dna sequences which enable the formation of modified starch in plant cells and plants, processes for the production of these plants and the modified starch obtainable therefrom
US6066782A (en) * 1993-09-09 2000-05-23 Hoechst Schering Agrevo Gmbh Combination of DNA sequences which enable the formation of modified starch in plant cells and plants, processes for the production of these plants and the modified starch obtainable therefrom
US5908975A (en) * 1993-11-09 1999-06-01 E. I. Du Pont De Nemours And Company Accumulation of fructans in plants by targeted expression of bacterial levansucrase
WO1995026407A1 (en) * 1994-03-25 1995-10-05 National Starch And Chemical Investment Holding Corporation Method for producing altered starch from potato plants
US6103893A (en) * 1994-03-25 2000-08-15 National Starch And Chemical Investment Holding Corporation High amylose starch from transgenic potato plants
US6265635B1 (en) 1994-05-18 2001-07-24 Plantec Biotechnologie Gmbh Forschung & Entwicklung DNA sequences coding for enzymes capable of facilitating the synthesis of linear α-1,4 glucans in plants, fungi and microorganisms
US6818421B2 (en) 1994-05-18 2004-11-16 Bayer Bioscience Gmbh DNA sequences coding for enzymes capable of facilitating the synthesis of linear α-1,4 glucans in plants, fungi and microorganisms
WO1995031553A1 (en) * 1994-05-18 1995-11-23 Institut Für Genbiologische Forschung Berlin Gmbh DNA SEQUENCES CODING FOR ENZYMES CAPABLE OF FACILITATING THE SYNTHESIS OF LINEAR α-1,4 GLUCANS IN PLANTS, FUNGI AND MICROORGANISMS
US6143562A (en) * 1995-04-06 2000-11-07 Seminis Vegetable Seeds Carbon-based process for selection of transgenic plant cells
US6825342B1 (en) 1995-05-05 2004-11-30 National Starch And Chemical Investment Holding Corporation Plant starch composition
US6974894B2 (en) 1995-05-05 2005-12-13 National Starch And Chemical Company Plant Starch Composition
WO1996034968A3 (en) * 1995-05-05 1996-12-05 Nat Starch Chem Invest Improvements in or relating to plant starch composition
WO1996034968A2 (en) * 1995-05-05 1996-11-07 National Starch And Chemical Investment Holding Corporation Improvements in or relating to plant starch composition
US6232122B1 (en) 1995-07-14 2001-05-15 Danisco A/S Inhibition of gene expression
WO1997004112A2 (en) * 1995-07-14 1997-02-06 Danisco A/S Inhibition of gene expression
WO1997004113A3 (en) * 1995-07-14 1997-05-15 Danisco Inhibition of gene expression
WO1997004112A3 (en) * 1995-07-14 1997-05-15 Danisco Inhibition of gene expression
AU709798B2 (en) * 1995-07-14 1999-09-09 Dupont Nutrition Biosciences Aps Inhibition of gene expression
US6147279A (en) * 1995-07-14 2000-11-14 Danisco A/S Inhibition of gene expression
WO1997004113A2 (en) * 1995-07-14 1997-02-06 Danisco A/S Inhibition of gene expression
US6207880B1 (en) 1995-09-19 2001-03-27 Planttec Biotechnologie Gmbh Plants which synthesize a modified starch, process for the production thereof and modified starch
US8586722B2 (en) 1995-09-19 2013-11-19 Bayer Cropscience Ag Methods of using tubers having genetically modified potato plant cells
EP1728441A3 (de) * 1995-09-19 2007-03-07 Bayer BioScience GmbH Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zu ihrer Herstellung sowie modifizierte Stärke
AU715944B2 (en) * 1995-09-19 2000-02-10 Bayer Cropscience Aktiengesellschaft Plants which synthesize a modified starch, process for the production thereof and modified starch
US7176190B2 (en) 1995-09-19 2007-02-13 Bayer Bioscience Gmbh Plants which synthesize a modified starch, process for the production thereof and modified starch
EP1702518A1 (de) * 1995-09-19 2006-09-20 Bayer BioScience GmbH Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zu ihrer Herstellung sowie modifizierte Stärke
EP1435205A1 (de) * 1995-09-19 2004-07-07 Bayer BioScience GmbH Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zu ihrer Herstellung sowie modifizierte Stärke
WO1997011188A1 (de) * 1995-09-19 1997-03-27 Planttec Biotechnologie Gmbh Pflanzen, die eine modifizierte stärke synthetisieren, verfahren zu ihrer herstellung sowie modifizierte stärke
US7569744B2 (en) 1995-09-19 2009-08-04 Bayer Bioscience Gmbh Plants which synthesize a modified starch, process for the production thereof and modified starch
US7897760B2 (en) 1995-09-19 2011-03-01 Bayer Bioscience Gmbh Plants which synthesize a modified starch, process for the production thereof and modified starch
US6815581B2 (en) 1995-09-19 2004-11-09 PlantTec Biotechnologies, GmbH Plants which synthesize a modified starch, process for the production thereof and modified starch
WO1998020145A3 (en) * 1996-11-05 1998-07-16 Nat Starch Chem Invest Improvements in or relating to starch content of plants
WO1998020145A2 (en) * 1996-11-05 1998-05-14 National Starch And Chemical Investment Holding Corporation Improvements in or relating to starch content of plants
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US6956148B1 (en) * 1996-11-05 2005-10-18 National Starch And Chemical Investment Holding Corporation Nucleic acids from cassava encoding starch branching enzyme II (SBEII) and their use
US7186898B1 (en) 1996-12-19 2007-03-06 Bayer Bioscience Gmbh Nucleic acid molecules from maize and their use for the production of modified starch
WO1998037213A1 (en) * 1997-02-21 1998-08-27 Danisco A/S Antisense intron inhibition of starch branching enzyme expression
US6483009B1 (en) 1997-02-21 2002-11-19 Danisco A/S Antisense intron inhibition of starch branching enzyme expression
US5998701A (en) * 1997-06-04 1999-12-07 Her Majesty The Queen In Right Of Canada As Represented By The Department Of Agriculture Potatoes having improved quality characteristics and methods for their production
US9029527B2 (en) 1998-03-20 2015-05-12 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs
US9963698B2 (en) 1998-03-20 2018-05-08 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
EP2267138A2 (en) 1998-04-08 2010-12-29 Commonwealth Scientific and Industrial Research Organization Methods and means for obtaining modified phenotypes
US9441239B2 (en) 1998-04-08 2016-09-13 Commonwealth Scientific & Industrial Research Organisation Methods and means for obtaining modified phenotypes
EP3214177A2 (en) 1998-04-08 2017-09-06 Commonwealth Scientific and Industrial Research Organisation Methods and means for obtaining modified phenotypes
US8598332B1 (en) 1998-04-08 2013-12-03 Bayer Cropscience N.V. Methods and means for obtaining modified phenotypes
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US7247769B2 (en) 1998-07-31 2007-07-24 Bayer Cropscience Gmbh Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
US6596928B1 (en) 1998-07-31 2003-07-22 Bayer Cropscience Gmbh Plants synthesizing a modified starch, the generation of the plants, their use, and the modified starch
US7385104B2 (en) 1998-07-31 2008-06-10 Bayer Cropscience Ag Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
DE19836098A1 (de) * 1998-07-31 2000-02-03 Hoechst Schering Agrevo Gmbh Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke
US7449623B2 (en) 1998-11-09 2008-11-11 Bayer Bioscience Gmbh Nucleic acid molecules from rice and their use for the production of modified starch
US6521816B1 (en) 1998-11-09 2003-02-18 Planttec Biotechnologie Gmbh Forschung Und Entwicklung Nucleic acid molecules from rice and their use for the production of modified starch
US9708621B2 (en) 1999-08-13 2017-07-18 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US10190127B2 (en) 1999-08-13 2019-01-29 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
CN100348617C (zh) * 2001-12-21 2007-11-14 拜尔作物科学有限责任公司 预胶凝化淀粉和它们的生产方法
US7045003B2 (en) 2001-12-21 2006-05-16 Bayer Cropscience Gmbh Pregelatinized starches and processes for their production
WO2003054024A1 (de) * 2001-12-21 2003-07-03 Bayer Cropscience Gmbh Quellstärken und verfahren zu deren herstellung
WO2003078629A1 (de) 2002-03-20 2003-09-25 Basf Plant Science Gmbh Konstrukte und verfahren zur regulation der genexpression
WO2004005516A1 (en) * 2002-07-09 2004-01-15 Basf Plant Science Gmbh Use of ahas mutant genes as selection marker in potato transformation
EP2202311A3 (en) * 2002-07-09 2010-07-28 BASF Plant Science GmbH Use of AHAS mutant genes as selection marker in potato transformation
EP2292768A1 (en) 2002-07-09 2011-03-09 BASF Plant Science GmbH Use of AHAS mutant genes as selection marker in potato transformation
EP2202311A2 (en) 2002-07-09 2010-06-30 BASF Plant Science GmbH Use of AHAS mutant genes as selection marker in potato transformation
EP2980220A1 (en) 2005-09-20 2016-02-03 BASF Plant Science GmbH Improved methods controlling gene expression
WO2013184768A1 (en) 2012-06-05 2013-12-12 University Of Georgia Research Foundation, Inc. Compositions and methods of gene silencing in plants
CN114703208A (zh) * 2022-01-21 2022-07-05 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) 马铃薯StGAPC基因在提高马铃薯淀粉含量上的用途

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Publication number Publication date
SE9004095L (sv) 1992-06-01
SE9004095D0 (sv) 1990-12-21
AU9109791A (en) 1992-07-22
PL169859B1 (pl) 1996-09-30
SE467160B (sv) 1992-06-01
EP0563201A1 (en) 1993-10-06

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