WO1991002074A1 - Procede d'obtention de polypeptides dans un systeme de translation exempt de cellules - Google Patents

Procede d'obtention de polypeptides dans un systeme de translation exempt de cellules Download PDF

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Publication number
WO1991002074A1
WO1991002074A1 PCT/SU1990/000144 SU9000144W WO9102074A1 WO 1991002074 A1 WO1991002074 A1 WO 1991002074A1 SU 9000144 W SU9000144 W SU 9000144W WO 9102074 A1 WO9102074 A1 WO 9102074A1
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WO
WIPO (PCT)
Prior art keywords
cell
ατρ
τρanslyatsii
ρηκ
sisτeme
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PCT/SU1990/000144
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English (en)
Russian (ru)
Inventor
Sergei Jurievich Ovodov
July Borisovich Alakhov
Original Assignee
Institut Belka Akademii Nauk Sssr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Institut Belka Akademii Nauk Sssr filed Critical Institut Belka Akademii Nauk Sssr
Publication of WO1991002074A1 publication Critical patent/WO1991002074A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Definitions

  • Polypeptides are widely used in medicine as a biological regulator in biological processes. For example, the known activists of the immune system, poliopeptides-neurotransmitters and transmitters, poliopeptides-mediators are removed. It is notable for the use of polypeptides in the agricultural sector as a means of growth, for example, the area of the rural area. Most of the drugs are also available in the bioelectronics industry, for example, in the form of a film with a pressure absorber.
  • the cartridge-free system accommodates the ⁇ C-8 in the cell for the installation of the “ ⁇ ⁇ ⁇ ⁇ ⁇ " ”system, equipped with an ⁇ -50 ultrafiltration membrane and the device is used for the synthesis of the device.
  • the themes of broadcasting are 10 nm of Calcite, which is 100 mmol of polyleltide per I mmol.
  • alkoxylating agents for example, diethyl pyro- bon, anhydrides of organic acids.
  • the proposed method ensures the synthesis of
  • ⁇ ig. I, 2, 3 show the dependencies of a combination of 20 synthesizable polypeptides per unit of synthesis time.
  • the resulting modifi- cate is added to the free system of broadcasting, which contains processes, initiation, elution, termination, aminoacyl, T-syntheses, amino acids, #
  • the system is placed in the cell for ultrasound, equipped with a user-friendly membrane with a diameter suitable for access to the target product.
  • the temperature sensor is at a standstill and the temperature is required at the same time, while the load is turned on at the same time as the outlet of the selected system.
  • the synthesis of the target product takes place at a constant speed.
  • Dependence on the quantity of the received interleukin per unit; the time of compression is presented in fig. 2.
  • 16 nanometers of the reagent were synthesized, which makes up 800 of the interleukine 2 on the first simulator.
  • the proposed service of 15 patients can be used in medicine, agriculture, agriculture.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On obtient des polypeptides sur des ribosomes dans un système de translation exempt de cellules contenant un ARN matriciel et, à titre de substrats, ATP, GTP ainsi que des acides aminés. Le processus de translation est basé sur l'emploi d'ARN matriciel modifié de 15 à 75 % par un agent d'alkylation, au niveau de 2'-hydroxyles de restes de ribose. Les produits de translation contenus dans le système comprennent des polypeptides, AMP, GDP, de la pyrophosphate ainsi que de la phosphate non organique. La consommation en substrats pendant le fonctionnement du système exempt de cellules, est compensée par l'alimentation de ce dernier en ATP, GTP et acides aminés à titre de substrats, afin de maintenir la concentration initiale en substrats.
PCT/SU1990/000144 1989-07-31 1990-06-05 Procede d'obtention de polypeptides dans un systeme de translation exempt de cellules WO1991002074A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SU4717701 1989-07-31
SU4717701/31 1989-07-31

Publications (1)

Publication Number Publication Date
WO1991002074A1 true WO1991002074A1 (fr) 1991-02-21

Family

ID=21460268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SU1990/000144 WO1991002074A1 (fr) 1989-07-31 1990-06-05 Procede d'obtention de polypeptides dans un systeme de translation exempt de cellules

Country Status (1)

Country Link
WO (1) WO1991002074A1 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988008453A1 (fr) * 1987-04-29 1988-11-03 Institut Belka Akademii Nauk Sssr Procede pour obtenir des polypeptides dans un systeme de translation acellulaire

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988008453A1 (fr) * 1987-04-29 1988-11-03 Institut Belka Akademii Nauk Sssr Procede pour obtenir des polypeptides dans un systeme de translation acellulaire

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BIOPOLIMERY I KLETKA, Vol. 4, No. 3, 1988, Naukova dumka (Kiev), A.P. POTAPOV et al., "Sravnitelnoe izuchenie matrichnoi aktivnosti poli(u) i poli (aT) v beskletochnylch beloksintezi-rujuschikh sistemakh iz escherichia coli i zarodyshei pshenitsy", pages 133-138. *
NUCLEIC ACIDS RESEARCH, Vol. 9, No. 18, 1981, IRL PRESS LIMITED (LONDON), JULIE M. PRATT, "Identification of Gene Products Programmed by Restriction Endonuclease DNA Fragments using an E. Coli in Vitro System", page 4459. *
SCIENCE, Vol. 242, 25 November 1988, A.S. SPIRIN et al., "A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield", pages 1162-1164. *
TRANSKRIPTSIA I TRANLYATSIA. METODY. POD RED. B. KHEIMSA, S. KHIGGINSA, 1987, Mir, (Moscow), pages 216-217. *

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