WO1987006596A2 - Process for affinity chromatographic separation of enzymes from aqueous solutions with the aid of solid carriers and specific ligands bound thereto - Google Patents

Process for affinity chromatographic separation of enzymes from aqueous solutions with the aid of solid carriers and specific ligands bound thereto Download PDF

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Publication number
WO1987006596A2
WO1987006596A2 PCT/EP1987/000214 EP8700214W WO8706596A2 WO 1987006596 A2 WO1987006596 A2 WO 1987006596A2 EP 8700214 W EP8700214 W EP 8700214W WO 8706596 A2 WO8706596 A2 WO 8706596A2
Authority
WO
WIPO (PCT)
Prior art keywords
aid
enzymes
aqueous solutions
solid carriers
chromatographic separation
Prior art date
Application number
PCT/EP1987/000214
Other languages
German (de)
English (en)
French (fr)
Other versions
WO1987006596A3 (fr
Inventor
Hans-Peter Call
Detlev MÜLLER-SCHULTE
Carl-Christian Emeis
Original Assignee
Call Hans Peter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Call Hans Peter filed Critical Call Hans Peter
Priority to JP62503809A priority Critical patent/JPH01500836A/ja
Priority to KR1019870701205A priority patent/KR880701253A/ko
Publication of WO1987006596A2 publication Critical patent/WO1987006596A2/de
Priority to DK668587A priority patent/DK668587A/da
Publication of WO1987006596A3 publication Critical patent/WO1987006596A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F291/00—Macromolecular compounds obtained by polymerising monomers on to macromolecular compounds according to more than one of the groups C08F251/00 - C08F289/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F2/00—Processes of polymerisation
    • C08F2/46—Polymerisation initiated by wave energy or particle radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/093—Polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01108—Lactase (3.2.1.108)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Graft Or Block Polymers (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
PCT/EP1987/000214 1986-04-21 1987-04-21 Process for affinity chromatographic separation of enzymes from aqueous solutions with the aid of solid carriers and specific ligands bound thereto WO1987006596A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP62503809A JPH01500836A (ja) 1987-04-21 1987-04-21 固体担体およびそれに結合した特定のリガンドによる、水溶液から酵素を分離するアフィニティークロマトグラフ法
KR1019870701205A KR880701253A (ko) 1986-04-21 1987-04-21 고체담체 및 그에 결합된 특수 배위자에 의해서 수용액으로부터 효소를 친화력 크로마토그래피적 분리방법
DK668587A DK668587A (da) 1987-04-21 1987-12-18 Fremgangsmaade til at skille enzymer fra en vandig oploesning

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3613407.4 1986-04-21
DE19863613407 DE3613407A1 (de) 1986-04-21 1986-04-21 Verfahren zur affinitaetschromatographischen trennung von peptiden (enzymen) aus waessrigen loesungen mit hilfe fester traeger und daran gebundener spezifischer liganden

Publications (2)

Publication Number Publication Date
WO1987006596A2 true WO1987006596A2 (en) 1987-11-05
WO1987006596A3 WO1987006596A3 (fr) 1988-04-07

Family

ID=6299151

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1987/000214 WO1987006596A2 (en) 1986-04-21 1987-04-21 Process for affinity chromatographic separation of enzymes from aqueous solutions with the aid of solid carriers and specific ligands bound thereto

Country Status (5)

Country Link
EP (1) EP0282496A1 (enrdf_load_html_response)
KR (1) KR880701253A (enrdf_load_html_response)
AU (1) AU7545587A (enrdf_load_html_response)
DE (1) DE3613407A1 (enrdf_load_html_response)
WO (1) WO1987006596A2 (enrdf_load_html_response)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19617731B4 (de) * 1995-07-29 2014-02-06 Johannes Schumacher Verfahren und Vorrichtung zur Bestimmung der Aktivität von Enzymen und Konzentration von Inhibitoren in Flüssigkeiten
EP0776374B1 (de) 1995-06-20 2009-12-16 Papst Licensing GmbH & Co. KG Verfahren und vorrichtung zur bestimmung der aktivität von enzymen in flüssigkeiten, beziehungsweise der konzentration und/oder aktivität von inhibitoren in flüssigkeiten

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1213873A (en) * 1967-03-02 1970-11-25 Japan Atomic Energy Res Inst Powder polyethylene adsorbent
DE2639129C3 (de) * 1976-08-31 1979-10-04 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig Verfahren zur Abtrennung von Enzymen

Also Published As

Publication number Publication date
EP0282496A1 (de) 1988-09-21
KR880701253A (ko) 1988-07-26
AU7545587A (en) 1987-11-24
DE3613407A1 (de) 1987-10-22
WO1987006596A3 (fr) 1988-04-07
DE3613407C2 (enrdf_load_html_response) 1992-05-21

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