WO1987006596A2 - Process for affinity chromatographic separation of enzymes from aqueous solutions with the aid of solid carriers and specific ligands bound thereto - Google Patents

Process for affinity chromatographic separation of enzymes from aqueous solutions with the aid of solid carriers and specific ligands bound thereto Download PDF

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Publication number
WO1987006596A2
WO1987006596A2 PCT/EP1987/000214 EP8700214W WO8706596A2 WO 1987006596 A2 WO1987006596 A2 WO 1987006596A2 EP 8700214 W EP8700214 W EP 8700214W WO 8706596 A2 WO8706596 A2 WO 8706596A2
Authority
WO
WIPO (PCT)
Prior art keywords
aid
enzymes
aqueous solutions
solid carriers
chromatographic separation
Prior art date
Application number
PCT/EP1987/000214
Other languages
German (de)
French (fr)
Other versions
WO1987006596A3 (en
Inventor
Hans-Peter Call
Detlev MÜLLER-SCHULTE
Carl-Christian Emeis
Original Assignee
Call Hans Peter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Call Hans Peter filed Critical Call Hans Peter
Priority to JP50380987A priority Critical patent/JPH01500836A/en
Priority to KR1019870701205A priority patent/KR880701253A/en
Publication of WO1987006596A2 publication Critical patent/WO1987006596A2/en
Priority to DK668587A priority patent/DK668587A/en
Publication of WO1987006596A3 publication Critical patent/WO1987006596A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F291/00—Macromolecular compounds obtained by polymerising monomers on to macromolecular compounds according to more than one of the groups C08F251/00 - C08F289/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F2/00—Processes of polymerisation
    • C08F2/46—Polymerisation initiated by wave energy or particle radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/093—Polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01108—Lactase (3.2.1.108)

Abstract

Grafting of vinyl monomers with functional attachable side groups with the aid of gamma rays or other radical-producing processes on an insoluble polymer substrate. Production of polymer carriers for biomolecules.
PCT/EP1987/000214 1986-04-21 1987-04-21 Process for affinity chromatographic separation of enzymes from aqueous solutions with the aid of solid carriers and specific ligands bound thereto WO1987006596A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP50380987A JPH01500836A (en) 1987-04-21 1987-04-21 Affinity chromatography method for separating enzymes from aqueous solutions using a solid support and specific ligands attached to it
KR1019870701205A KR880701253A (en) 1986-04-21 1987-04-21 Affinity chromatographic separation of enzymes from aqueous solutions by solid carriers and special ligands bound thereto
DK668587A DK668587A (en) 1987-04-21 1987-12-18 PROCEDURE FOR Separating Enzymes from Aqueous Solution

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19863613407 DE3613407A1 (en) 1986-04-21 1986-04-21 METHOD FOR THE AFFINITY CHROMATOGRAPHIC SEPARATION OF PEPTIDES (ENZYMES) FROM AQUEOUS SOLUTIONS WITH THE AID OF SOLID CARRIERS AND TIED SPECIFIC LIGANDS

Publications (2)

Publication Number Publication Date
WO1987006596A2 true WO1987006596A2 (en) 1987-11-05
WO1987006596A3 WO1987006596A3 (en) 1988-04-07

Family

ID=6299151

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1987/000214 WO1987006596A2 (en) 1986-04-21 1987-04-21 Process for affinity chromatographic separation of enzymes from aqueous solutions with the aid of solid carriers and specific ligands bound thereto

Country Status (5)

Country Link
EP (1) EP0282496A1 (en)
KR (1) KR880701253A (en)
AU (1) AU7545587A (en)
DE (1) DE3613407A1 (en)
WO (1) WO1987006596A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19617731B4 (en) * 1995-07-29 2014-02-06 Johannes Schumacher Method and device for determining the activity of enzymes and concentration of inhibitors in liquids
US5935846A (en) 1995-06-20 1999-08-10 Schumacher; Johannes Device for determining the activity of enzymes in liquids

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1567220A (en) * 1967-03-02 1969-05-16
DE2639129A1 (en) * 1976-08-31 1978-03-02 Biotechnolog Forschung Gmbh Enzymes sepn. from cells after solubilisation - by partition in multiphase systems contg. polymers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1567220A (en) * 1967-03-02 1969-05-16
DE2639129A1 (en) * 1976-08-31 1978-03-02 Biotechnolog Forschung Gmbh Enzymes sepn. from cells after solubilisation - by partition in multiphase systems contg. polymers

Also Published As

Publication number Publication date
EP0282496A1 (en) 1988-09-21
KR880701253A (en) 1988-07-26
WO1987006596A3 (en) 1988-04-07
DE3613407A1 (en) 1987-10-22
DE3613407C2 (en) 1992-05-21
AU7545587A (en) 1987-11-24

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