Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/06—Lysis of microorganisms
  • C12N1/066—Lysis of microorganisms by physical methods
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
  • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
  • B02C19/00—Other disintegrating devices or methods
  • B02C19/18—Use of auxiliary physical effects, e.g. ultrasonics, irradiation, for disintegrating

Definitions

• the present invention relates to an ultrasonic crushing device, and more particularly to a cell crushing device used to break down cell membranes of cells and to separate organelles in the cells.
• the present invention relates to an ultrasonic crushing apparatus having a special feature in a container for storing a solution of cells to be broken.
• the vibrator is excited by the output of the oscillator, and the ultrasonic vibration is applied to the red blood cells in the container via the horn, causing a cavity phenomenon and the like. It is a device that crushes cell membranes such as red blood cells. This The conventional device fills a container with a large amount of crushed cells, etc., and gives them a more uniform ultrasonic wave than the horn. Although it is possible to descend, on a glare surface, in the case of the conventional type, the horn remains inserted in the container, so it is necessary to keep the container open and an unsealed groove Because of its construction, when treating a toxic body, the ultrasonic treatment may cause the body to be aerosolized and scattered in many parts. ⁇ It's not something that researchers like.
• the container may be contaminated by external bacteria and microorganisms.
• the resulting organelles cannot be maintained in a pure state, which may hinder the intended research.
• the water contained in the breached cells is easily ii efficient due to the non-sealed groove structure as described above, it is easy to cause a change in In many cases, no results can be obtained.
• biochemical analysis of the internal fluid of red blood cells and white blood cells should be avoided as much as possible. The one who has not responded well to the request.
• the fluid generated by cell destruction that is, the organelles? Use a centrifuge to separate the cloudy night (split into solid organelles, but since this is a valley with an unsealed groove, it is dispensed into a container And then centrifuged.
• O PI Temperature has a great relationship with ⁇ -dispersion of ultrasonic vibrations and changes in the sand itself, such as cells, and proper control of the liquid temperature is required in the disposal operation.
• the liquid is cooled from the outside of the container and the liquid temperature is measured, and the control of the liquid is difficult and difficult to manage. There was.
• the dispersibility of ultrasonic vibrations is good when the liquid temperature is fi: good, and most of the torn objects are stored at a temperature of ⁇ . If the temperature is significantly higher than this, thermal changes will occur and the desired breakdown result will not be obtained, so in these cases the night temperature is always cooled.
• the conventional type cools from the outside of the container, so it could not be cooled as intended o
• the conventional type is suitable for assaulting the Ito-type destructible body, as described above, at the time of I-ji, but on the other hand, it is a single destruction ⁇ It is suitable for breaking ⁇ efficiently.
• the present invention has been made in view of these points, and its purpose is to make it possible to wave the cell and to keep the cell in a sealed state. You need to This not only enables researchers and workers to be safe, but also protects the organelles resulting from the rupture from contamination by external bacteria and microorganisms. The goal is to minimize changes in the cells during the rupture. Ultrasonic vibration is also used to enable the night temperature of the seeding tank to be directly buried. As a result, it is easy to embed the liquid temperature, which has a great influence on fe dissemination of ultrasonic vibration, etc. In order to efficiently break down the cells. and
• the present invention is capable of accommodating the lysing of blast cells.
• the vessel is immersed in a vertical manner at night in the row, and ultrasonic waves are oscillated from the bottom of the row to crush the cells in the vessel.
• ⁇ ⁇ Has a bottomed cylindrical shape
• the liquid temperature of the room where the ultrasonic vibration is sowed can be directly buried, the night temperature can be easily controlled, and the ultrasonic vibration and dissemination can be improved. You can do what you want. In addition, a small number of cells can be efficiently destroyed. Also, it is a metal tube, and
• the jab was inserted into or returned from the exit
• FIG. 1 is a longitudinal side view of the apparatus, ⁇ 2 figures sectional view of the container of the row of ⁇ 1, FIG. 3 is a second ⁇ volume ⁇ of It is sectional drawing.
• the present invention relates to a container A provided in such an ultrasonic sonication device, and first, a second example will be described.
• Vessel A is a bottomed cylindrical metallic Chi-menu Bed 6, a key Ya Tsu Bed 8 you seal the opening ⁇ 7 metallic Chi-menu Bed 6 is supported in the center of the key Ya class tap 8 Consisting of nine gold mist sticks.
• the metallic tube 6 is made of stainless steel, and the top of the opening 7 is bent outward and the flange 10 is bent.
• the cap 8 is a metal tube.
• small-diameter portion 11 is because we Shi was inserted into the open ⁇ part in 7 of the probe 6, Ri some Ri good large-diameter portion I 2 sit on the open mouth portion 7 outside of the full run-di-10, top Symbol small-diameter portion 11 It has a 0 ring wound around it.
• the I port 7 of the metal tube 6 is formed by both the 0 ring around the small diameter portion and the large diameter portion I 2 ′ sitting on the flange 10. And sealed. Further, the gold cap 9 is attached to the cap 8 as follows. In other words, the gold hole 9 was left in the center hole of the cap 8, and the nut was attached to the female screw I 4 formed at the top of the metal wedge 9. It is supported by hanging nuts.
• the cap 8 is removed, and the metal tube 6 contains the solution T containing the cells to be broken.
• the cap 8 is worn. That is, the small-diameter portion 11 is inserted into the opening 7 by a person, and the large-diameter portion I 2 is placed on the flange 10.
• the 0 ring 13 is in close contact with the inner peripheral surface of the gold tube 6, and the bottom surface of the large section I 2 is seated on the flange 10.
• the inside of the metal tube 6 is sealed.
• the metal rod 9 is supported with its tip IS hanging downward with a distance of 1 ⁇ kana ⁇ from the bottom of the metal rod 9 to the cell.
• the tip W is located in the solution in which is dissolved.
• the other breakdown action is the length, thickness, and overall shape of the container ⁇ such that the container ⁇ has a 'natural vibration' that resonates with the ultrasonic vibration.
• all the j $ of container A resonates, that is, the metal tube 6 , the cap 8 and the captive 9
• the ultrasonic vibration is applied to the cells by the tip IS of the metal element 9, and a cavitation phenomenon occurs in the solution in which the cells are dissolved, and this cavitation occurs.
• an instantaneous depressing force and a compressive force are alternately applied to a liquid, thereby causing the cell membrane of each cell to be broken. It is.
• the breakdown was performed using a device with an ultrasonic frequency of 29 KHZ, a power of 110 W, a power supply of 50 y 60 EZ 100 V, and a liquid temperature in the 'tff of 6 TC. .
• the liquid W in the bath 4 can be directly cooled.
• vessel A itself is small and can set diplomacy in ft
• the cell suspension thus obtained is centrifuged as it is.
• a female screw 23 is formed on the outer peripheral surface of the opening 22.
• the inner portion 26 is a portion that can be attached to the sales opening 22, and other
• a male screw 28 is formed on the surface of the outer portion 27.
• cap 2 male screw 28 is fitted with a metal tube.

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• Health & Medical Sciences (AREA)
• Engineering & Computer Science (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Genetics & Genomics (AREA)
• Organic Chemistry (AREA)
• Biotechnology (AREA)
• Zoology (AREA)
• Wood Science & Technology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Medicinal Chemistry (AREA)
• General Health & Medical Sciences (AREA)
• Microbiology (AREA)
• Virology (AREA)
• Tropical Medicine & Parasitology (AREA)
• Biochemistry (AREA)
• General Engineering & Computer Science (AREA)
• Biomedical Technology (AREA)
• Mycology (AREA)
• Toxicology (AREA)
• Food Science & Technology (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)
• Investigating Or Analysing Biological Materials (AREA)
• Disintegrating Or Milling (AREA)
• Micro-Organisms Or Cultivation Processes Thereof (AREA)
• Sampling And Sample Adjustment (AREA)

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ID=16154913

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Cited By (3)

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• 1982
  • 1982-12-06 JP JP1982184536U patent/JPS5987669U/ja active Granted
• 1983
  • 1983-12-05 US US06/695,455 patent/US4697751A/en not_active Expired - Fee Related
  • 1983-12-05 WO PCT/JP1983/000427 patent/WO1984002350A1/ja unknown

Patent Citations (1)

Non-Patent Citations (1)

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