WO1984002193A1 - Chromogenic support immunoassay - Google Patents

Chromogenic support immunoassay Download PDF

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Publication number
WO1984002193A1
WO1984002193A1 PCT/US1983/001887 US8301887W WO8402193A1 WO 1984002193 A1 WO1984002193 A1 WO 1984002193A1 US 8301887 W US8301887 W US 8301887W WO 8402193 A1 WO8402193 A1 WO 8402193A1
Authority
WO
WIPO (PCT)
Prior art keywords
support
analyte
immunoassay
antibody
enzyme
Prior art date
Application number
PCT/US1983/001887
Other languages
English (en)
French (fr)
Inventor
Richard Calvin Ebersole
John William Delouche Eveleigh
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of WO1984002193A1 publication Critical patent/WO1984002193A1/en
Priority to DK375984A priority Critical patent/DK375984A/da

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/528—Atypical element structures, e.g. gloves, rods, tampons, toilet paper
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

Definitions

  • U.S. Patent 4.200,690 issued April 29. 1980 to Root et al., discloses an immunoassay for an intestinal parasite in which the test result is measured by the color which forms on a support.
  • the chromogenic substance used is poorly soluble in the reaction solvent or is preferentially entrapped in the filter matrix in the colored state. Again, precipitation on or preferential binding of the chromogen to the support is largely fortuitous.
  • the assay of this invention can also be performed on filter such as a starch coated glass fiber filter.
  • filter such as a starch coated glass fiber filter.
  • the patient sample can be applied directly to the filter to which antibodies have been previously attached.
  • the antibody utilized in the enzyme-labeled antibody conjugate can be the same as that used to coat the support or it can be an antibody directed to different antigenic determinant on the analyte.
  • the assay of this invention utilizes a nonimmunochemical capture of the analyte. This capture can occur through the physical forces described above or can be mediated by, for example, lectins on the support.
  • the lectins can act in at least two different functions.
  • a lectin can capture all of the microorganisms from a biological sample for which the lectin has specificity.
  • the antianalyte antibody-enzyme conjugate acts to identify the microorganism sought.
  • the lectin itself can serve to capture selectively the microorganism of interest (see footnote 2 above). The microorganism so immobilized on the active support can then be detected by the immunoassay of this invention.
  • the conjugation of the enzyme label to an antibody can be carried out by known methods and in such a way as to minimize both the loss of immunoreactivity of the antibody and the loss of activity of the label. Attachment can be direct or indirect, via a spacer arm. Usually, it is necessary to purify the conjugate extensively before use in the assay in order to remove any unreacted label, unlabeled antibody, and nonimmunoreactive labeled antibody. This can be accomplished by a combination of gel filtration and affinity chromatography steps.
  • the conjugate can be an enzyme-labeled anti-analyte antibody or an enzyme-labeled antibody which is an antibody to an anti-analyte antibody.
  • the product itself is a colored substance; for example, chromogenic substrates such as para-nitrophenyl phosphate and ortho-nitrophenyl galactoside are known for many common enzymes. Chromogen/substrate pairs are also known, for exmple, peroxides and ortho-phenylenediamine for horseradish peroxidase.
  • the product can be less visible or substantially colorless but can become more visible or colored upon interaction with the support.
  • the support can be a starch-coated material which can complex with iodine generated as the product of an enzyme-catalyzed reaction to yield a characteristic blue color on the support.
  • the nonlinearity of color intensity observed was due to the prozone effect, that is, only a single dilution of antibody was utilized in the assay.
  • the control for this assay was performed as above but without HSV antigen.
  • Swabs Composed of polysaccharide fibers such as cotton, flax, hemp, jute, and rayon
  • They are washed with water and then air dried. They are placed in 5 M potassium phosphate buffer. pH 11.9 (25 mL buffer per 1 g of cotton fiber), to which 2 mL of a cyanogen bromide solution (100 mg/mL) is added in small aliquots with gentle stirring over a 2-minute period at 5-10°C. The temperature is maintained for an additional 8 minutes at 5-10°C after which the swabs are washed thoroughly with water and allowed to air dry.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PCT/US1983/001887 1982-12-03 1983-12-02 Chromogenic support immunoassay WO1984002193A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK375984A DK375984A (da) 1982-12-03 1984-08-02 Chromogen immunanalyse paa understoetning

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44643482A 1982-12-03 1982-12-03
US44643382A 1982-12-03 1982-12-03

Publications (1)

Publication Number Publication Date
WO1984002193A1 true WO1984002193A1 (en) 1984-06-07

Family

ID=27034622

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1983/001887 WO1984002193A1 (en) 1982-12-03 1983-12-02 Chromogenic support immunoassay

Country Status (3)

Country Link
EP (1) EP0126772A1 (pm)
GR (1) GR79120B (pm)
WO (1) WO1984002193A1 (pm)

Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0212599A3 (en) * 1985-08-28 1987-08-19 Miles Laboratories, Inc. Multizone analytical element having detectable signal concentrating zone
EP0191640A3 (en) * 1985-02-14 1987-08-19 Syntex (U.S.A.) Inc. Concentrating immunochemical test strip
FR2599845A1 (fr) * 1986-06-09 1987-12-11 Liotta Lance A Dispositif pour la determination de la presence d'antigenes dans un echantillon et procede pour sa mise en oeuvre
WO1989001162A1 (en) * 1987-07-28 1989-02-09 Biotechnology Australia Pty. Ltd. Detection methods
WO1990009593A1 (en) * 1989-02-09 1990-08-23 Eastman Kodak Company Method and kit for determination of herpes simplex viral antigen by direct binding to polymeric particles
US4959307A (en) * 1986-09-05 1990-09-25 Syntex (U.S.A.) Inc. Immunoseparating strip
US4963468A (en) * 1986-09-05 1990-10-16 Syntex (U.S.A.) Inc. Immunoseparating strip
EP0231010A3 (en) * 1986-01-27 1990-10-17 INCSTAR Corporation A method of solid phase enzyme immunoassay and nucleic acid hybridization assay and dip-stick design and stabilized chromogenic substrate
WO1990011523A3 (en) * 1989-03-29 1991-02-21 Du Pont Catalyzed reporter deposition
EP0369361A3 (en) * 1988-11-17 1991-06-05 Becton, Dickinson and Company Immunoassay on a preblocked solid surface
US5030558A (en) * 1986-11-07 1991-07-09 Syntex (U.S.A.) Inc. Qualitative immunochromatographic method and device
US5085988A (en) * 1986-09-05 1992-02-04 Syntex (U.S.A.) Inc. Immunoseparating strip
EP0402993A3 (en) * 1989-06-14 1992-03-18 Johnson & Johnson Clinical Diagnostics, Inc. Diagnostic test kit and method for determination of chlamydial antigen using a membrane having surface hydroxy groups
WO1992021772A1 (en) * 1991-05-30 1992-12-10 Abbott Laboratories Ion-capture assays using a binding member conjugated to carboxymethylamylose
US5196306A (en) * 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
US5260194A (en) * 1986-09-05 1993-11-09 Syntex (U.S.A.) Inc. Immunoseparating strip
US5260193A (en) * 1986-09-05 1993-11-09 Syntex (U.S.A.) Inc. Immunoseparating strip
US5459080A (en) * 1988-01-29 1995-10-17 Abbott Laboratories Ion-capture assays using a specific binding member conjugated to carboxymethylamylose
US5459078A (en) * 1988-01-29 1995-10-17 Abbott Laboratories Methods and reagents for performing ion-capture digoxin assays
US5866322A (en) * 1988-01-29 1999-02-02 Abbott Laboratories Method for performing Rubella assay
US6887377B2 (en) 2001-02-01 2005-05-03 Sigma-Aldrich Co. Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications
US9128082B2 (en) 2009-03-24 2015-09-08 Biocept, Inc. Devices and methods of cell capture and analysis
US9671407B2 (en) 2009-03-24 2017-06-06 Biocept, Inc. Devices and methods of cell capture and analysis
US9739773B1 (en) 2010-08-13 2017-08-22 David Gordon Bermudes Compositions and methods for determining successful immunization by one or more vaccines
US10865235B2 (en) 2011-07-18 2020-12-15 President And Fellows Of Harvard College Engineered microbe-targeting molecules and uses thereof
US11034744B2 (en) 2013-12-18 2021-06-15 President And Fellows Of Harvard College CRP capture/detection of gram positive bacteria
US11059874B2 (en) 2010-01-19 2021-07-13 President And Fellows Of Harvard College Engineered opsonin for pathogen detection and treatment
US11312949B2 (en) 2013-05-21 2022-04-26 President And Fellows Of Harvard College Engineered heme-binding compositions and uses thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4071315A (en) * 1976-06-02 1978-01-31 Guy Chateau Analytical tape medium
WO1980002800A1 (en) * 1979-06-21 1980-12-24 G Oster Specific impending ovulation indicator
US4289747A (en) * 1978-12-26 1981-09-15 E-Y Laboratories, Inc. Immunological determination using lectin
US4299916A (en) * 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays
US4322495A (en) * 1980-03-11 1982-03-30 Minnesota Mining And Manufacturing Co. Immunoassay
US4391904A (en) * 1979-12-26 1983-07-05 Syva Company Test strip kits in immunoassays and compositions therein
US4407943A (en) * 1976-12-16 1983-10-04 Millipore Corporation Immobilized antibody or antigen for immunoassay

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4071315A (en) * 1976-06-02 1978-01-31 Guy Chateau Analytical tape medium
US4407943A (en) * 1976-12-16 1983-10-04 Millipore Corporation Immobilized antibody or antigen for immunoassay
US4289747A (en) * 1978-12-26 1981-09-15 E-Y Laboratories, Inc. Immunological determination using lectin
WO1980002800A1 (en) * 1979-06-21 1980-12-24 G Oster Specific impending ovulation indicator
US4299916A (en) * 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays
US4391904A (en) * 1979-12-26 1983-07-05 Syva Company Test strip kits in immunoassays and compositions therein
US4322495A (en) * 1980-03-11 1982-03-30 Minnesota Mining And Manufacturing Co. Immunoassay

Cited By (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0191640A3 (en) * 1985-02-14 1987-08-19 Syntex (U.S.A.) Inc. Concentrating immunochemical test strip
EP0422699A3 (en) * 1985-02-14 1991-05-08 Syntex (U.S.A.) Inc. Methods for binding a compound on bibulous material
US5716778A (en) * 1985-02-14 1998-02-10 Abbott Laboratories Concentrating immunochemical test device and method
EP0212599A3 (en) * 1985-08-28 1987-08-19 Miles Laboratories, Inc. Multizone analytical element having detectable signal concentrating zone
EP0231010A3 (en) * 1986-01-27 1990-10-17 INCSTAR Corporation A method of solid phase enzyme immunoassay and nucleic acid hybridization assay and dip-stick design and stabilized chromogenic substrate
BE1001736A4 (nl) * 1986-06-09 1990-02-20 Liotta Lance A Gelaagde immunoassay waarbij antilichamen worden gebruikt die gebonden zijn aan transportdeeltjes, voor het bepalen van de aanwezigheid van een antigeen.
FR2599845A1 (fr) * 1986-06-09 1987-12-11 Liotta Lance A Dispositif pour la determination de la presence d'antigenes dans un echantillon et procede pour sa mise en oeuvre
US4959307A (en) * 1986-09-05 1990-09-25 Syntex (U.S.A.) Inc. Immunoseparating strip
US4963468A (en) * 1986-09-05 1990-10-16 Syntex (U.S.A.) Inc. Immunoseparating strip
US5260194A (en) * 1986-09-05 1993-11-09 Syntex (U.S.A.) Inc. Immunoseparating strip
US5085988A (en) * 1986-09-05 1992-02-04 Syntex (U.S.A.) Inc. Immunoseparating strip
US5260193A (en) * 1986-09-05 1993-11-09 Syntex (U.S.A.) Inc. Immunoseparating strip
US5030558A (en) * 1986-11-07 1991-07-09 Syntex (U.S.A.) Inc. Qualitative immunochromatographic method and device
WO1989001162A1 (en) * 1987-07-28 1989-02-09 Biotechnology Australia Pty. Ltd. Detection methods
US5866322A (en) * 1988-01-29 1999-02-02 Abbott Laboratories Method for performing Rubella assay
US5459078A (en) * 1988-01-29 1995-10-17 Abbott Laboratories Methods and reagents for performing ion-capture digoxin assays
US5459080A (en) * 1988-01-29 1995-10-17 Abbott Laboratories Ion-capture assays using a specific binding member conjugated to carboxymethylamylose
EP0369361A3 (en) * 1988-11-17 1991-06-05 Becton, Dickinson and Company Immunoassay on a preblocked solid surface
US6235464B1 (en) 1988-11-17 2001-05-22 Glenn L. Henderson Immunoassay on a preblocked solid surface
WO1990009593A1 (en) * 1989-02-09 1990-08-23 Eastman Kodak Company Method and kit for determination of herpes simplex viral antigen by direct binding to polymeric particles
US5196306A (en) * 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
US5583001A (en) * 1989-03-29 1996-12-10 E. I. Du Pont De Nemours And Company Method for detection or quantitation of an analyte using an analyte dependent enzyme activation system
WO1990011523A3 (en) * 1989-03-29 1991-02-21 Du Pont Catalyzed reporter deposition
US5731158A (en) * 1989-03-29 1998-03-24 E. I. Du Pont De Nemours And Company Catalyzed reporter deposition
EP0402993A3 (en) * 1989-06-14 1992-03-18 Johnson & Johnson Clinical Diagnostics, Inc. Diagnostic test kit and method for determination of chlamydial antigen using a membrane having surface hydroxy groups
WO1992021772A1 (en) * 1991-05-30 1992-12-10 Abbott Laboratories Ion-capture assays using a binding member conjugated to carboxymethylamylose
US6887377B2 (en) 2001-02-01 2005-05-03 Sigma-Aldrich Co. Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications
US7163633B2 (en) 2001-02-01 2007-01-16 Sigma-Aldrich Co. Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications
US7438806B2 (en) 2001-02-01 2008-10-21 Sigma-Aldrich Co. Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications
EP2411808B1 (en) * 2009-03-24 2015-11-11 Biocept, Inc. Devices and methods of cell capture and analysis
US9128082B2 (en) 2009-03-24 2015-09-08 Biocept, Inc. Devices and methods of cell capture and analysis
US9671407B2 (en) 2009-03-24 2017-06-06 Biocept, Inc. Devices and methods of cell capture and analysis
US11719692B2 (en) 2009-03-24 2023-08-08 Biocept, Inc. Devices and methods of cell capture and analysis
US10527611B2 (en) 2009-03-24 2020-01-07 Biocept, Inc. Devices and methods of cell capture and analysis
US11059874B2 (en) 2010-01-19 2021-07-13 President And Fellows Of Harvard College Engineered opsonin for pathogen detection and treatment
US11059873B2 (en) 2010-01-19 2021-07-13 President And Fellows Of Harvard College Engineered opsonin for pathogen detection and treatment
US11203623B2 (en) 2010-01-19 2021-12-21 President And Fellows Of Harvard College Engineered opsonin for pathogen detection and treatment
US9739773B1 (en) 2010-08-13 2017-08-22 David Gordon Bermudes Compositions and methods for determining successful immunization by one or more vaccines
US10865235B2 (en) 2011-07-18 2020-12-15 President And Fellows Of Harvard College Engineered microbe-targeting molecules and uses thereof
US11795212B2 (en) 2011-07-18 2023-10-24 President And Fellows Of Harvard College Engineered microbe-targeting molecules and uses thereof
US11312949B2 (en) 2013-05-21 2022-04-26 President And Fellows Of Harvard College Engineered heme-binding compositions and uses thereof
US11939608B2 (en) 2013-05-21 2024-03-26 President And Fellows Of Harvard College Engineered heme-binding compositions and uses thereof
US11034744B2 (en) 2013-12-18 2021-06-15 President And Fellows Of Harvard College CRP capture/detection of gram positive bacteria
US11718651B2 (en) 2013-12-18 2023-08-08 President And Fellows Of Harvard College CRP capture/detection of gram positive bacteria

Also Published As

Publication number Publication date
GR79120B (pm) 1984-10-02
EP0126772A1 (en) 1984-12-05

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