WO1984002193A1 - Analyse immunologique a support chromogene - Google Patents
Analyse immunologique a support chromogene Download PDFInfo
- Publication number
- WO1984002193A1 WO1984002193A1 PCT/US1983/001887 US8301887W WO8402193A1 WO 1984002193 A1 WO1984002193 A1 WO 1984002193A1 US 8301887 W US8301887 W US 8301887W WO 8402193 A1 WO8402193 A1 WO 8402193A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- support
- analyte
- immunoassay
- antibody
- enzyme
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/521—Single-layer analytical elements
- G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/528—Atypical element structures, e.g. gloves, rods, tampons, toilet paper
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
Definitions
- U.S. Patent 4.200,690 issued April 29. 1980 to Root et al., discloses an immunoassay for an intestinal parasite in which the test result is measured by the color which forms on a support.
- the chromogenic substance used is poorly soluble in the reaction solvent or is preferentially entrapped in the filter matrix in the colored state. Again, precipitation on or preferential binding of the chromogen to the support is largely fortuitous.
- the assay of this invention can also be performed on filter such as a starch coated glass fiber filter.
- filter such as a starch coated glass fiber filter.
- the patient sample can be applied directly to the filter to which antibodies have been previously attached.
- the antibody utilized in the enzyme-labeled antibody conjugate can be the same as that used to coat the support or it can be an antibody directed to different antigenic determinant on the analyte.
- the assay of this invention utilizes a nonimmunochemical capture of the analyte. This capture can occur through the physical forces described above or can be mediated by, for example, lectins on the support.
- the lectins can act in at least two different functions.
- a lectin can capture all of the microorganisms from a biological sample for which the lectin has specificity.
- the antianalyte antibody-enzyme conjugate acts to identify the microorganism sought.
- the lectin itself can serve to capture selectively the microorganism of interest (see footnote 2 above). The microorganism so immobilized on the active support can then be detected by the immunoassay of this invention.
- the conjugation of the enzyme label to an antibody can be carried out by known methods and in such a way as to minimize both the loss of immunoreactivity of the antibody and the loss of activity of the label. Attachment can be direct or indirect, via a spacer arm. Usually, it is necessary to purify the conjugate extensively before use in the assay in order to remove any unreacted label, unlabeled antibody, and nonimmunoreactive labeled antibody. This can be accomplished by a combination of gel filtration and affinity chromatography steps.
- the conjugate can be an enzyme-labeled anti-analyte antibody or an enzyme-labeled antibody which is an antibody to an anti-analyte antibody.
- the product itself is a colored substance; for example, chromogenic substrates such as para-nitrophenyl phosphate and ortho-nitrophenyl galactoside are known for many common enzymes. Chromogen/substrate pairs are also known, for exmple, peroxides and ortho-phenylenediamine for horseradish peroxidase.
- the product can be less visible or substantially colorless but can become more visible or colored upon interaction with the support.
- the support can be a starch-coated material which can complex with iodine generated as the product of an enzyme-catalyzed reaction to yield a characteristic blue color on the support.
- the nonlinearity of color intensity observed was due to the prozone effect, that is, only a single dilution of antibody was utilized in the assay.
- the control for this assay was performed as above but without HSV antigen.
- Swabs Composed of polysaccharide fibers such as cotton, flax, hemp, jute, and rayon
- They are washed with water and then air dried. They are placed in 5 M potassium phosphate buffer. pH 11.9 (25 mL buffer per 1 g of cotton fiber), to which 2 mL of a cyanogen bromide solution (100 mg/mL) is added in small aliquots with gentle stirring over a 2-minute period at 5-10°C. The temperature is maintained for an additional 8 minutes at 5-10°C after which the swabs are washed thoroughly with water and allowed to air dry.
Abstract
Analyse immunologique utilisant un support actif pouvant concentrer un signal détectable résultant de l'interaction du produit d'une réaction entre un complexe immun étiqueté par une enzyme et les substrats de l'enzyme avec le support. Le complexe immun est soit formé soit recueilli sur le support actif pendant l'analyse. L'analyse est utile pour la détection et la mesure d'analytes dans des échantillons biologiques.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK375984A DK375984D0 (da) | 1982-12-03 | 1984-08-02 | Chromogen immunanalyse paa understoetning |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US44643482A | 1982-12-03 | 1982-12-03 | |
US44643382A | 1982-12-03 | 1982-12-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1984002193A1 true WO1984002193A1 (fr) | 1984-06-07 |
Family
ID=27034622
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1983/001887 WO1984002193A1 (fr) | 1982-12-03 | 1983-12-02 | Analyse immunologique a support chromogene |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP0126772A1 (fr) |
GR (1) | GR79120B (fr) |
WO (1) | WO1984002193A1 (fr) |
Cited By (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0191640A2 (fr) * | 1985-02-14 | 1986-08-20 | Abbott Laboratories | Bande d'essai immunochimique concentrante |
EP0212599A2 (fr) * | 1985-08-28 | 1987-03-04 | Miles Inc. | Elément analytique multicouche pour des essais immunologiques ayant une couche pour concentrer le signal détectable |
EP0231010A2 (fr) * | 1986-01-27 | 1987-08-05 | INCSTAR Corporation | Procédé pour un essai immuno-enzymique en phase solide et essai d'hybridisation des acides nucléiques et dessin d'un bâtonnet d'échantillon et substrat chromogène stabilisé |
FR2599845A1 (fr) * | 1986-06-09 | 1987-12-11 | Liotta Lance A | Dispositif pour la determination de la presence d'antigenes dans un echantillon et procede pour sa mise en oeuvre |
WO1989001162A1 (fr) * | 1987-07-28 | 1989-02-09 | Biotechnology Australia Pty. Ltd. | Procedes de detection |
EP0369361A2 (fr) * | 1988-11-17 | 1990-05-23 | Becton, Dickinson and Company | Essai immunologique sur une surface solide prébloquée |
WO1990009593A1 (fr) * | 1989-02-09 | 1990-08-23 | Eastman Kodak Company | Procede et kit de determination d'antigene viral de l'herpes par liaison directe a des particules polymeres |
US4959307A (en) * | 1986-09-05 | 1990-09-25 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
WO1990011523A2 (fr) * | 1989-03-29 | 1990-10-04 | E.I. Du Pont De Nemours And Company | Depôt de rapport par catalysation |
US4963468A (en) * | 1986-09-05 | 1990-10-16 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
EP0402993A2 (fr) * | 1989-06-14 | 1990-12-19 | Johnson & Johnson Clinical Diagnostics, Inc. | Trousse d'essai diagnostique et procédé pour la détermination de l'antigène chlamydia utilisant une membrane ayant des groupes hydroxyles à la surface |
US5030558A (en) * | 1986-11-07 | 1991-07-09 | Syntex (U.S.A.) Inc. | Qualitative immunochromatographic method and device |
US5085988A (en) * | 1986-09-05 | 1992-02-04 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
WO1992021772A1 (fr) * | 1991-05-30 | 1992-12-10 | Abbott Laboratories | Analyses avec capture d'ions faisant appel a un element de fixation associe a du carboxymethylamylose |
US5196306A (en) * | 1989-03-29 | 1993-03-23 | E. I. Du Pont De Nemours And Company | Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system |
US5260194A (en) * | 1986-09-05 | 1993-11-09 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
US5260193A (en) * | 1986-09-05 | 1993-11-09 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
US5459080A (en) * | 1988-01-29 | 1995-10-17 | Abbott Laboratories | Ion-capture assays using a specific binding member conjugated to carboxymethylamylose |
US5459078A (en) * | 1988-01-29 | 1995-10-17 | Abbott Laboratories | Methods and reagents for performing ion-capture digoxin assays |
US5866322A (en) * | 1988-01-29 | 1999-02-02 | Abbott Laboratories | Method for performing Rubella assay |
US6887377B2 (en) | 2001-02-01 | 2005-05-03 | Sigma-Aldrich Co. | Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications |
US9128082B2 (en) | 2009-03-24 | 2015-09-08 | Biocept, Inc. | Devices and methods of cell capture and analysis |
US9671407B2 (en) | 2009-03-24 | 2017-06-06 | Biocept, Inc. | Devices and methods of cell capture and analysis |
US9739773B1 (en) | 2010-08-13 | 2017-08-22 | David Gordon Bermudes | Compositions and methods for determining successful immunization by one or more vaccines |
US10865235B2 (en) | 2011-07-18 | 2020-12-15 | President And Fellows Of Harvard College | Engineered microbe-targeting molecules and uses thereof |
US11034744B2 (en) | 2013-12-18 | 2021-06-15 | President And Fellows Of Harvard College | CRP capture/detection of gram positive bacteria |
US11059874B2 (en) | 2010-01-19 | 2021-07-13 | President And Fellows Of Harvard College | Engineered opsonin for pathogen detection and treatment |
US11312949B2 (en) | 2013-05-21 | 2022-04-26 | President And Fellows Of Harvard College | Engineered heme-binding compositions and uses thereof |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4071315A (en) * | 1976-06-02 | 1978-01-31 | Guy Chateau | Analytical tape medium |
WO1980002800A1 (fr) * | 1979-06-21 | 1980-12-24 | G Oster | Indicateur specifique d'ovulation imminente |
US4289747A (en) * | 1978-12-26 | 1981-09-15 | E-Y Laboratories, Inc. | Immunological determination using lectin |
US4299916A (en) * | 1979-12-26 | 1981-11-10 | Syva Company | Preferential signal production on a surface in immunoassays |
US4322495A (en) * | 1980-03-11 | 1982-03-30 | Minnesota Mining And Manufacturing Co. | Immunoassay |
US4391904A (en) * | 1979-12-26 | 1983-07-05 | Syva Company | Test strip kits in immunoassays and compositions therein |
US4407943A (en) * | 1976-12-16 | 1983-10-04 | Millipore Corporation | Immobilized antibody or antigen for immunoassay |
-
1983
- 1983-12-02 WO PCT/US1983/001887 patent/WO1984002193A1/fr not_active Application Discontinuation
- 1983-12-02 EP EP19840900222 patent/EP0126772A1/fr not_active Withdrawn
- 1983-12-05 GR GR73155A patent/GR79120B/el unknown
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4071315A (en) * | 1976-06-02 | 1978-01-31 | Guy Chateau | Analytical tape medium |
US4407943A (en) * | 1976-12-16 | 1983-10-04 | Millipore Corporation | Immobilized antibody or antigen for immunoassay |
US4289747A (en) * | 1978-12-26 | 1981-09-15 | E-Y Laboratories, Inc. | Immunological determination using lectin |
WO1980002800A1 (fr) * | 1979-06-21 | 1980-12-24 | G Oster | Indicateur specifique d'ovulation imminente |
US4299916A (en) * | 1979-12-26 | 1981-11-10 | Syva Company | Preferential signal production on a surface in immunoassays |
US4391904A (en) * | 1979-12-26 | 1983-07-05 | Syva Company | Test strip kits in immunoassays and compositions therein |
US4322495A (en) * | 1980-03-11 | 1982-03-30 | Minnesota Mining And Manufacturing Co. | Immunoassay |
Cited By (51)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0191640A3 (en) * | 1985-02-14 | 1987-08-19 | Syntex (U.S.A.) Inc. | Concentrating immunochemical test strip |
US5716778A (en) * | 1985-02-14 | 1998-02-10 | Abbott Laboratories | Concentrating immunochemical test device and method |
EP0191640A2 (fr) * | 1985-02-14 | 1986-08-20 | Abbott Laboratories | Bande d'essai immunochimique concentrante |
EP0422699A3 (en) * | 1985-02-14 | 1991-05-08 | Syntex (U.S.A.) Inc. | Methods for binding a compound on bibulous material |
EP0422699A2 (fr) * | 1985-02-14 | 1991-04-17 | Abbott Laboratories | Méthodes pour la fixation d'un composé à matière absorbante |
EP0212599A2 (fr) * | 1985-08-28 | 1987-03-04 | Miles Inc. | Elément analytique multicouche pour des essais immunologiques ayant une couche pour concentrer le signal détectable |
EP0212599A3 (en) * | 1985-08-28 | 1987-08-19 | Miles Laboratories, Inc. | Multizone analytical element having detectable signal concentrating zone |
EP0231010A3 (fr) * | 1986-01-27 | 1990-10-17 | INCSTAR Corporation | Procédé pour un essai immuno-enzymique en phase solide et essai d'hybridisation des acides nucléiques et dessin d'un bâtonnet d'échantillon et substrat chromogène stabilisé |
EP0231010A2 (fr) * | 1986-01-27 | 1987-08-05 | INCSTAR Corporation | Procédé pour un essai immuno-enzymique en phase solide et essai d'hybridisation des acides nucléiques et dessin d'un bâtonnet d'échantillon et substrat chromogène stabilisé |
FR2599845A1 (fr) * | 1986-06-09 | 1987-12-11 | Liotta Lance A | Dispositif pour la determination de la presence d'antigenes dans un echantillon et procede pour sa mise en oeuvre |
BE1001736A4 (nl) * | 1986-06-09 | 1990-02-20 | Liotta Lance A | Gelaagde immunoassay waarbij antilichamen worden gebruikt die gebonden zijn aan transportdeeltjes, voor het bepalen van de aanwezigheid van een antigeen. |
US5260193A (en) * | 1986-09-05 | 1993-11-09 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
US5085988A (en) * | 1986-09-05 | 1992-02-04 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
US5260194A (en) * | 1986-09-05 | 1993-11-09 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
US4963468A (en) * | 1986-09-05 | 1990-10-16 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
US4959307A (en) * | 1986-09-05 | 1990-09-25 | Syntex (U.S.A.) Inc. | Immunoseparating strip |
US5030558A (en) * | 1986-11-07 | 1991-07-09 | Syntex (U.S.A.) Inc. | Qualitative immunochromatographic method and device |
WO1989001162A1 (fr) * | 1987-07-28 | 1989-02-09 | Biotechnology Australia Pty. Ltd. | Procedes de detection |
US5866322A (en) * | 1988-01-29 | 1999-02-02 | Abbott Laboratories | Method for performing Rubella assay |
US5459080A (en) * | 1988-01-29 | 1995-10-17 | Abbott Laboratories | Ion-capture assays using a specific binding member conjugated to carboxymethylamylose |
US5459078A (en) * | 1988-01-29 | 1995-10-17 | Abbott Laboratories | Methods and reagents for performing ion-capture digoxin assays |
EP0369361A3 (fr) * | 1988-11-17 | 1991-06-05 | Becton, Dickinson and Company | Essai immunologique sur une surface solide prébloquée |
US6235464B1 (en) | 1988-11-17 | 2001-05-22 | Glenn L. Henderson | Immunoassay on a preblocked solid surface |
EP0369361A2 (fr) * | 1988-11-17 | 1990-05-23 | Becton, Dickinson and Company | Essai immunologique sur une surface solide prébloquée |
WO1990009593A1 (fr) * | 1989-02-09 | 1990-08-23 | Eastman Kodak Company | Procede et kit de determination d'antigene viral de l'herpes par liaison directe a des particules polymeres |
US5731158A (en) * | 1989-03-29 | 1998-03-24 | E. I. Du Pont De Nemours And Company | Catalyzed reporter deposition |
WO1990011523A3 (fr) * | 1989-03-29 | 1991-02-21 | Du Pont | Depôt de rapport par catalysation |
US5196306A (en) * | 1989-03-29 | 1993-03-23 | E. I. Du Pont De Nemours And Company | Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system |
WO1990011523A2 (fr) * | 1989-03-29 | 1990-10-04 | E.I. Du Pont De Nemours And Company | Depôt de rapport par catalysation |
US5583001A (en) * | 1989-03-29 | 1996-12-10 | E. I. Du Pont De Nemours And Company | Method for detection or quantitation of an analyte using an analyte dependent enzyme activation system |
EP0402993A3 (fr) * | 1989-06-14 | 1992-03-18 | Johnson & Johnson Clinical Diagnostics, Inc. | Trousse d'essai diagnostique et procédé pour la détermination de l'antigène chlamydia utilisant une membrane ayant des groupes hydroxyles à la surface |
EP0402993A2 (fr) * | 1989-06-14 | 1990-12-19 | Johnson & Johnson Clinical Diagnostics, Inc. | Trousse d'essai diagnostique et procédé pour la détermination de l'antigène chlamydia utilisant une membrane ayant des groupes hydroxyles à la surface |
WO1992021772A1 (fr) * | 1991-05-30 | 1992-12-10 | Abbott Laboratories | Analyses avec capture d'ions faisant appel a un element de fixation associe a du carboxymethylamylose |
US6887377B2 (en) | 2001-02-01 | 2005-05-03 | Sigma-Aldrich Co. | Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications |
US7163633B2 (en) | 2001-02-01 | 2007-01-16 | Sigma-Aldrich Co. | Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications |
US7438806B2 (en) | 2001-02-01 | 2008-10-21 | Sigma-Aldrich Co. | Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications |
EP2411808B1 (fr) * | 2009-03-24 | 2015-11-11 | Biocept, Inc. | Dispositifs et procédés de capture et d'analyse de cellules |
US9671407B2 (en) | 2009-03-24 | 2017-06-06 | Biocept, Inc. | Devices and methods of cell capture and analysis |
US10527611B2 (en) | 2009-03-24 | 2020-01-07 | Biocept, Inc. | Devices and methods of cell capture and analysis |
US9128082B2 (en) | 2009-03-24 | 2015-09-08 | Biocept, Inc. | Devices and methods of cell capture and analysis |
US11719692B2 (en) | 2009-03-24 | 2023-08-08 | Biocept, Inc. | Devices and methods of cell capture and analysis |
US11203623B2 (en) | 2010-01-19 | 2021-12-21 | President And Fellows Of Harvard College | Engineered opsonin for pathogen detection and treatment |
US11059874B2 (en) | 2010-01-19 | 2021-07-13 | President And Fellows Of Harvard College | Engineered opsonin for pathogen detection and treatment |
US11059873B2 (en) | 2010-01-19 | 2021-07-13 | President And Fellows Of Harvard College | Engineered opsonin for pathogen detection and treatment |
US9739773B1 (en) | 2010-08-13 | 2017-08-22 | David Gordon Bermudes | Compositions and methods for determining successful immunization by one or more vaccines |
US10865235B2 (en) | 2011-07-18 | 2020-12-15 | President And Fellows Of Harvard College | Engineered microbe-targeting molecules and uses thereof |
US11795212B2 (en) | 2011-07-18 | 2023-10-24 | President And Fellows Of Harvard College | Engineered microbe-targeting molecules and uses thereof |
US11312949B2 (en) | 2013-05-21 | 2022-04-26 | President And Fellows Of Harvard College | Engineered heme-binding compositions and uses thereof |
US11939608B2 (en) | 2013-05-21 | 2024-03-26 | President And Fellows Of Harvard College | Engineered heme-binding compositions and uses thereof |
US11034744B2 (en) | 2013-12-18 | 2021-06-15 | President And Fellows Of Harvard College | CRP capture/detection of gram positive bacteria |
US11718651B2 (en) | 2013-12-18 | 2023-08-08 | President And Fellows Of Harvard College | CRP capture/detection of gram positive bacteria |
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GR79120B (fr) | 1984-10-02 |
EP0126772A1 (fr) | 1984-12-05 |
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