UY38770A - Combinación de cebadores para un ensayo de pcr múltiple para soja transgénica, un kit y su uso - Google Patents

Combinación de cebadores para un ensayo de pcr múltiple para soja transgénica, un kit y su uso

Info

Publication number
UY38770A
UY38770A UY0001038770A UY38770A UY38770A UY 38770 A UY38770 A UY 38770A UY 0001038770 A UY0001038770 A UY 0001038770A UY 38770 A UY38770 A UY 38770A UY 38770 A UY38770 A UY 38770A
Authority
UY
Uruguay
Prior art keywords
primer combination
kit
epsps
gat
transgenic
Prior art date
Application number
UY0001038770A
Other languages
English (en)
Inventor
Lijuan Qiu
Jing Wen
Yong Guo
Original Assignee
Inst Of Crop Sciences Chinese Academy Of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Of Crop Sciences Chinese Academy Of Agricultural Sciences filed Critical Inst Of Crop Sciences Chinese Academy Of Agricultural Sciences
Publication of UY38770A publication Critical patent/UY38770A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención describe una combinación de cebadores para el ensayo de PCR múltiple para soja transgénica, un kit y su uso. En particular, la combinación de cebadores incluye cebadores de referencia internos GmActin11 F y GmActin11 R, cebadores de genes extraños G2-EPSPS F, G2-EPSPS R, GAT F y GAT R, y cebadores de secuencia flanqueante ZH10P1, GAT-2, G2- EPSPS-2 y ZH10P2. Se describe un kit que incluye la combinación de cebadores anterior. Los métodos de aplicación incluyen un sistema de reacción detallado y condiciones de PCR. La presente invención es económica, rápida y fácil de operar, con una sensibilidad del 0,5 %. La presente invención cumple con los requisitos de la UE para la identificación de productos transgénicos y puede usarse para la detección de la soja transgénica ZH10-6 y las líneas derivadas de esta.
UY0001038770A 2019-07-03 2020-06-29 Combinación de cebadores para un ensayo de pcr múltiple para soja transgénica, un kit y su uso UY38770A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910596303.7A CN110343779A (zh) 2019-07-03 2019-07-03 多重pcr检测转基因大豆的引物组合、试剂盒及应用

Publications (1)

Publication Number Publication Date
UY38770A true UY38770A (es) 2021-02-26

Family

ID=68178123

Family Applications (1)

Application Number Title Priority Date Filing Date
UY0001038770A UY38770A (es) 2019-07-03 2020-06-29 Combinación de cebadores para un ensayo de pcr múltiple para soja transgénica, un kit y su uso

Country Status (2)

Country Link
CN (1) CN110343779A (es)
UY (1) UY38770A (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112029892A (zh) * 2020-09-16 2020-12-04 天津市农业科学院 一种快速鉴定转基因耐除草剂大豆zh10-6转化体特异性的方法
CN112176087A (zh) * 2020-09-30 2021-01-05 中国农业科学院生物技术研究所 转基因大豆中黄6106的rpa检测引物与探针组合、试剂盒及检测方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090011938A1 (en) * 2000-10-30 2009-01-08 Pioneer Hi--Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
CN103614413B (zh) * 2013-11-21 2015-08-12 中国农业科学院作物科学研究所 表面活性剂与超声波协同提高大豆遗传转化效率的方法及其应用
CN106350532A (zh) * 2016-08-28 2017-01-25 浙江大学 一种抗草甘膦融合基因、编码蛋白及其应用
CN106995841B (zh) * 2017-03-20 2020-10-09 苏州大学 一种转基因大豆检测用多重pcr试剂盒及检测方法

Also Published As

Publication number Publication date
CN110343779A (zh) 2019-10-18

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