UY25133A1 - Tolerancia a las microtoxinas tricotecenicas en vegetales a traves de la modificacion del gen de la proteina ribosomica l3. - Google Patents

Tolerancia a las microtoxinas tricotecenicas en vegetales a traves de la modificacion del gen de la proteina ribosomica l3.

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Publication number
UY25133A1
UY25133A1 UY25133A UY25133A UY25133A1 UY 25133 A1 UY25133 A1 UY 25133A1 UY 25133 A UY25133 A UY 25133A UY 25133 A UY25133 A UY 25133A UY 25133 A1 UY25133 A1 UY 25133A1
Authority
UY
Uruguay
Prior art keywords
gene
trichothecenes
trichothecene
mycotoxins
fusarium
Prior art date
Application number
UY25133A
Other languages
English (en)
Inventor
J Harris Linda
C Gleddie Stephen
A Simmonds John
Original Assignee
Her Majesty In Right Of Canada As Represented By The Mini Of Agriculture And Agri Food Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Her Majesty In Right Of Canada As Represented By The Mini Of Agriculture And Agri Food Canada filed Critical Her Majesty In Right Of Canada As Represented By The Mini Of Agriculture And Agri Food Canada
Publication of UY25133A1 publication Critical patent/UY25133A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

Un gen modificado, cuya forma de tipo salvaje codifica a una proteína ribosomal l3 y donde un huésped transformado por dicho gen modificado tiene mayor resistencia a las microtoxinas tricotecénicas, con la previsión de que dicho gen no es de la Saccharomyces cerevisiae. El Fusarim graminearum es un fitopatógeno que ataca a una gran variedad de especies vegetales, incluyendo al maíz (putrefacción de la mazorca y del tallo), la cebada y el trigo (añublo de las espigas). Las epidemias de Fusarium provocan millones de dólares en pérdidas en las cosechas de cultivos. La infección por Fusarim graminearum en cereales reduce tanto el rendimiento en granos como su calidad. Las micotoxinas son producidas por muchas especies de hongos Fusarium y, por ende, los granos se contaminan con estas micotoxinas, tales como los tricotecenos. El principal tricotecenos producido por el F. Gramineum, así como por el F. culmorum, es el deoxinivalenol (que se abrevia como DON y es tambíen comocido como vomitoxina). Los tricotecenos son potentes inhibidores de la síntesis proteica y son bastante tóxico para los seres humanos y para el ganado. Se ha identificado un gen de levadura que es resistente al tricoteceno, la tricodermina. Se ha preparado un gen vegetal correspondiente, el que se ha utilizado para transformar plantas y sería apropiado para transformar animales. Estas plantas transformadas tienen una mayor tolerancia a los tricotecenos y podrían ser más resistentes a la infección por Fusarium. Potencialmente,los animales transformados podrían tener mayor tolerancia a las micotoxinas tricotecenicas. Este gen modificado podría también utilizarse como marcador selectivo en experimentos de transformación.
UY25133A 1997-08-12 1998-08-11 Tolerancia a las microtoxinas tricotecenicas en vegetales a traves de la modificacion del gen de la proteina ribosomica l3. UY25133A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/909,828 US6060646A (en) 1997-08-12 1997-08-12 Tolerance of trichothecene mycotoxins in plants and animals through the modification of the peptidyl transferase gene

Publications (1)

Publication Number Publication Date
UY25133A1 true UY25133A1 (es) 1998-09-14

Family

ID=25427893

Family Applications (1)

Application Number Title Priority Date Filing Date
UY25133A UY25133A1 (es) 1997-08-12 1998-08-11 Tolerancia a las microtoxinas tricotecenicas en vegetales a traves de la modificacion del gen de la proteina ribosomica l3.

Country Status (9)

Country Link
US (1) US6060646A (es)
EP (1) EP1003867A1 (es)
CN (1) CN1268974A (es)
AR (1) AR016788A1 (es)
AU (1) AU754959B2 (es)
BR (1) BR9815603A (es)
CA (1) CA2300681C (es)
UY (1) UY25133A1 (es)
WO (1) WO1999009173A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6855872B2 (en) * 1997-08-12 2005-02-15 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Tolerance of trichothecene mycotoxins in plants through the modification of the ribosomal protein L3 gene
AU2401800A (en) * 1998-12-31 2000-07-31 Rutgers, The State University Virus-resistant transgenic plants expressing l3
TR200102573T2 (tr) * 1999-03-31 2002-04-22 Syngenta Participations Ag Transgenik bitki ve metotları.
US6346655B1 (en) * 1999-03-31 2002-02-12 Syngenta Participations Ag Trichothecne-Resistant transgenic plants
AT408442B (de) * 1999-07-19 2001-11-26 Innovationsagentur Gmbh Mutiertes ribosomales protein l3
CN1301747A (zh) * 1999-12-27 2001-07-04 上海博德基因开发有限公司 一种新的多肽——核糖体s19e蛋白10和编码这种多肽的多核苷酸
US20060005271A1 (en) * 2003-12-12 2006-01-05 Rutgers, The State University Transgenic plants expressing L3 delta proteins are resistant to trichothecene fungal toxins
CN1318427C (zh) * 2005-03-21 2007-05-30 中国海洋大学 氢化木霉醇类化合物及其制备方法和用途

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816406A (en) * 1986-07-24 1989-03-28 The United States Of America, As Represented By The Secretary Of Agriculture Inhibition of trichothecene toxins by ancymidol
DE4108746A1 (de) * 1991-03-18 1992-09-24 Lindner Wolfgang Dekontamination und detoxifikation von getreide, welches mit trochothecen-mykotoxinen belastet ist
DE69332148T2 (de) * 1992-12-07 2003-03-27 Biogemma Paris Verwendung einer dna sequenz, kodierende für ein protein mit der fäligkeit oxalsäure abzubauen, zur genselektion
US5589611A (en) * 1992-12-15 1996-12-31 Pioneer Hi-Bred International, Inc. Disease resistance gene from maize and its use for disease resistance as a selectable marker and as a gene identification probe
US5792931A (en) * 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
EP0800345B1 (en) * 1994-12-30 2002-04-03 Proguard, Inc. Toxic metabolite modulation in consumable products

Also Published As

Publication number Publication date
CA2300681A1 (en) 1999-02-25
AU754959B2 (en) 2002-11-28
BR9815603A (pt) 2001-01-16
US6060646A (en) 2000-05-09
CA2300681C (en) 2007-07-03
EP1003867A1 (en) 2000-05-31
AU8526398A (en) 1999-03-08
AR016788A1 (es) 2001-08-01
CN1268974A (zh) 2000-10-04
WO1999009173A1 (en) 1999-02-25

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Effective date: 20180811