USRE48510E1 - Phosphorus functional antimicrobial coatings for metal surfaces - Google Patents

Phosphorus functional antimicrobial coatings for metal surfaces Download PDF

Info

Publication number
USRE48510E1
USRE48510E1 US15/938,151 US201415938151A USRE48510E US RE48510 E1 USRE48510 E1 US RE48510E1 US 201415938151 A US201415938151 A US 201415938151A US RE48510 E USRE48510 E US RE48510E
Authority
US
United States
Prior art keywords
compound
formula
nmr
ethyl
phosphonate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
US15/938,151
Other languages
English (en)
Inventor
Lukasz POROSA
Gideon WOLFAARDT
Daniel Foucher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NANO SAFE COATINGS Inc
Original Assignee
NANO SAFE COATINGS Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NANO SAFE COATINGS Inc filed Critical NANO SAFE COATINGS Inc
Priority to US15/938,151 priority Critical patent/USRE48510E1/en
Assigned to NANO SAFE COATINGS INCORPORATED reassignment NANO SAFE COATINGS INCORPORATED ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BIOSHIELD TECHNOLOGIES CANADA LIMITED
Assigned to BIOSHIELD TECHNOLOGIES CANADA LIMITED reassignment BIOSHIELD TECHNOLOGIES CANADA LIMITED ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: WOLFAARDT, Gideon, FOUCHER, DANIEL, POROSA, Lukasz
Application granted granted Critical
Publication of USRE48510E1 publication Critical patent/USRE48510E1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/40—Esters thereof
    • C07F9/4003—Esters thereof the acid moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/4006—Esters of acyclic acids which can have further substituents on alkyl
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/02—Amines; Quaternary ammonium compounds
    • A01N33/12—Quaternary ammonium compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D3/00—Pretreatment of surfaces to which liquids or other fluent materials are to be applied; After-treatment of applied coatings, e.g. intermediate treating of an applied coating preparatory to subsequent applications of liquids or other fluent materials
    • B05D3/02—Pretreatment of surfaces to which liquids or other fluent materials are to be applied; After-treatment of applied coatings, e.g. intermediate treating of an applied coating preparatory to subsequent applications of liquids or other fluent materials by baking
    • B05D3/0254—After-treatment
    • B05D3/0272—After-treatment with ovens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/3804—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)] not used, see subgroups
    • C07F9/3808—Acyclic saturated acids which can have further substituents on alkyl
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/3804—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)] not used, see subgroups
    • C07F9/3808—Acyclic saturated acids which can have further substituents on alkyl
    • C07F9/3817—Acids containing the structure (RX)2P(=X)-alk-N...P (X = O, S, Se)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/3804—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)] not used, see subgroups
    • C07F9/3839—Polyphosphonic acids
    • C07F9/3873—Polyphosphonic acids containing nitrogen substituent, e.g. N.....H or N-hydrocarbon group which can be substituted by halogen or nitro(so), N.....O, N.....S, N.....C(=X)- (X =O, S), N.....N, N...C(=X)...N (X =O, S)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/40—Esters thereof
    • C07F9/4003—Esters thereof the acid moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/4006—Esters of acyclic acids which can have further substituents on alkyl
    • C07F9/4009—Esters containing the structure (RX)2P(=X)-alk-N...P (X = O, S, Se)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
    • C07F9/572—Five-membered rings
    • C07F9/5728—Five-membered rings condensed with carbocyclic rings or carbocyclic ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
    • C07F9/6552—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a six-membered ring
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
    • C09D5/14—Paints containing biocides, e.g. fungicides, insecticides or pesticides
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
    • C09D5/16—Antifouling paints; Underwater paints
    • C09D5/1606—Antifouling paints; Underwater paints characterised by the anti-fouling agent
    • C09D5/1612—Non-macromolecular compounds
    • C09D5/1625—Non-macromolecular compounds organic

Definitions

  • Bacterial infections in hospital environments are spread by two different ways: external contamination or in vivo contamination from implants. Patients can develop external infections through contact with surfaces such as door handles, pens, telephones, health care workers uniforms (“HCWU”), stethoscopes, or sterile packaging that have been colonized by microorganisms. Hospital-acquired infections (“HAI”) from contact with pathogenic microorganisms affect approximately 2 million people and result in more than 100,000 deaths in the U.S.A. each year. Such infections require 10-20 days of additional patient hospitalization, costing the already strained U.S. health-care systems approximately $25,000-30,000 per infection totaling billions of dollars per year.
  • HAI health care workers uniforms
  • IAI Implant associated infections
  • U.S. per year For example, approximately 10-50% of patients with implanted catheters run the risk of developing urinary tract infections (“UTI”) resulting in additional healthcare costs.
  • UTI urinary tract infections
  • HAI's and IAI's can be attributed to decreased antibiotic efficacy against drug-resistant strains of pathogens found in surface biofilms.
  • Biofilm formation involves three phases beginning with the initial reversible adhesion of bacteria on a surface through polysaccharides and adhesion proteins on the bacterial membrane (phase I). Under appropriate conditions, bacteria subsequently firmly attach to a surface (phase II), followed by the secretion of a protective polymeric matrix (biofilm, phase III) in which the bacteria typically show a marked increase in resistance to antibiotics, compared to none-adherent bacteria. As a result, once the infection occurs, it becomes difficult to treat. Thus, strategies that prevent bacterial contamination or destroy adsorbed microorganisms that lead to biofilm formation are actively sought.
  • Phosphonate monolayers for the antimicrobial treatment of surfaces have been shown to be advantageous over self-assembled monolayers (SAMs) of thiols and silanes in terms of durability, long-term stability and surface coverage, especially on titanium and stainless steel.
  • SAMs self-assembled monolayers
  • Thiol-based SAM's lack substrate specificity (mainly reserved for gold surfaces) and long-term stability needed for biomedical applications, (i.e. implants). Over time, the thiol-based SAM's become oxidized to sulfonates, which lack affinity for gold and become displaced from the surface.
  • phosphonate based SAM's are advantageous because they resist hydrolysis under physiological conditions and higher surface coverage can be obtained without harsh acid surface pretreatment (to increase the OH content).
  • Siloxanes are also known to be unstable and are easily hydrolyzed under physiological conditions.
  • U.S. Pat. No. 4,101,654 teaches phosphonate-pendant nitrogen heterocyclic compounds that are quaternized by alkyl halides and their use as corrosion inhibitor compounds.
  • U.S. Pat. No. 4,420,399 teaches phosphonate-quaternary ammonium compounds having a methylene group linking the phosphorus and nitrogen atoms and their use as corrosion inhibitor compounds.
  • U.S. Pat. No. 5,770,586 teaches phosphonate/phosphoric acid-quaternary ammonium compounds for use as dental care ingredients and for bone density treatment.
  • U.S. Pat. No. 5,888,405 teaches methods of inhibiting bacteria from adhering to submerged surfaces using amino-phosphonic acid compounds.
  • PCT Application Publication WO 2007/080291 teaches bisphosphonate-amines and quaternary ammonium compounds, their preparation and attachment to metal and metal-oxide surfaces and testing for antibacterial activity.
  • PCT Application Publication WO 2008/017721 teaches bisphosphonate-amines and quaternary ammonium compounds, their preparation and attachment to silicon and metal surfaces and cell proliferation testing.
  • R 1 and R 2 are independently lower alkyl groups preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably selected from methyl, ethyl, isopropyl or n-propyl groups, even more preferably methyl groups, m is 15, 16, 17, 18 or 19, n is 0, 1, 2, 3, 4, 5 or 6, and X is chloro, bromo or iodo, comprising the steps of (a) reacting a compound of formula (II)
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, with an alkyl halide of formula (III)
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably selected from methyl, ethyl, isopropyl or n-propyl groups, even more preferably methyl groups, and m is 15, 16, 17, 18 or 19, to give a compound of formula (VI)
  • the process may take place neat or in a polar, protic reaction solvent, preferably a lower alkanol selected from methanol, ethanol and isopropanol. The process may be carried out at the refluxing temperature of the reaction solvent. The process is considered complete when the compound of formula (VI) is no longer observable via thin-layer chromatography.
  • the compound of formula (I) may be purified, preferably by chromatography or recrystallization.
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, and Z is chloro, bromo or hydroxy, preferably bromo
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl
  • m is 15, 16, 17, 18 or 19
  • n is 0, 1, 2, 3, 4, 5, or 6, and o is 1, 2 or 3, comprising the steps of (a) reacting, preferably at least two equivalents of compound of formula (IX)
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, per equivalent of a compound of formula (X)
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, n is 0, 1, 2, 3, 4, 5, or 6, o is 1, 2 or 3 and Z is selected from chloro, bromo or hydroxyl, preferably bromo, with p-toluenesulfonyl chloride, trimethyl ammonium chloride, trimethylamine in a polar, aprotic solvent preferably acetonitrile, dimethylformamide or dichloromethane, more preferably dichloromethane, (b) adding a compound R 1 R 2 NH where R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl, in a polar, protic solvent selected from methanol, ethanol or isopropanol optionally in the presence of water, to give a compound of formula (XII)
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl or hydrogen and even more preferably hydrogen
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl groups
  • m is 15, 16, 17, 18 or 19
  • n is 0, 1, 2, 3, 4, 5, or 6
  • Z is selected from chloro or bromo, preferably bromo, comprising the steps of (a) reacting a compound of formula (XV) wherein R is defined as above
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, with a compound of formula (XVI)
  • n is as defined above with at least one equivalent of O ⁇ PH(OR) 2 where R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, in the presence of an alkali metal carbonate, preferably potassium carbonate, methanesulfonyl chloride and an organic amine base, and further reacted with sodium hydride and at least a second equivalent of O ⁇ PH(OR) 2 to give a compound of formula (XIX)
  • R is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, and n is 0, 1, 2, 3, 4, 5 or 6, comprising the steps of reacting a compound of formula (XX)
  • R is methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl
  • R 1 and R 2 are each independently a lower alkyl group preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl groups
  • n is 0, 1, 2, 3, 4, 5 or 6, comprising reacting a compound of formula (XXIII)
  • a bis-phosphonate compound of formula (XXIV) and a process for preparing a compound of formula (XXIV) where R is hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, and n is 0, 1, 2, 3, 4, 5 or 6,
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl or hydrogen and even more preferably hydrogen
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl groups
  • m is 15, 16, 17, 18 or 19
  • Z is selected from chloro or bromo, preferably bromo, comprising the steps of (a) reacting oxalyl chloride with
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, and R 1 and R 2 are as defined above, and (b) reacting the compound of formula (XXVII) with a compound of formula (XIII)
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl or hydrogen and even more preferably hydrogen
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl groups
  • n is 0, 1, 2, 3, 4, 5 or 6
  • p is 0, 1, 2, 3, 4, 5 or 6
  • Z is selected from chloro or bromo, preferably bromo, comprising the steps of (a) reacting a compound of (XXIX)
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, and n and Z are as defined above, in a polar, aprotic solvent to give a compound of formula (XXVIII).
  • the processes may take place neat or in polar, aprotic reaction solvents, for example but not limited to dichloromethane, acetonitrile and dimethylformamide.
  • aprotic reaction solvents for example but not limited to dichloromethane, acetonitrile and dimethylformamide.
  • the processes may take place in a lower alkanol preferably methanol, ethanol and isopropanol.
  • the processes may be carried out at temperatures from about ⁇ 80° C. to about 150° C. The process is considered complete when the starting material is no longer observable via thin-layer chromatography.
  • the final products optionally may be purified, preferably by chromatography or recrystallization.
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl or hydrogen and even more preferably hydrogen
  • m is 15, 16, 17, 18 or 19
  • Z is selected from chloro or bromo, preferably bromo, comprising the steps of: a) alkylating a tetralkyl methylenebisphosphonate (TAMBP) compound, mono-deprotecting TAMBP followed by mono alkylation to lead to alpha (C—H) bisphosphonates, and performing a second deprotonation/alkylation with dialkyl chlorophosphate to provide trisphosphonates; b) Michael addition of dialkyl vinylphosphite to provide beta aminobisphosphonates and further deprotonation and phosphorylation with dialkyl chlorophosphate to provide tetraphosphonates; or c) Lewis acid-mediated Abrzov addition of trialkylphosphite
  • an antimicrobial composition comprising any one of a compound of formulae (I), (VII), (XIV), (XXVI), (XXVIII), (XXXIII), (XXXIV), (XXXV), (XXXVI) and (XXXVII) and a process for treating a surface with an antimicrobial coating comprising the steps of contacting the surface with a composition comprising any one of a compound of formulae (I), (VII), (XIV), (XXVI), (XXVIII), (XXXIII), (XXXIV), (XXXV), (XXXVI) and (XXXVII).
  • a phosphonate antimicrobial coating composition for treating surfaces to give a stable and durable phosphonate antimicrobial coating surface treatment, said composition comprising any one of a compound of formulae (I), (VII), (XIV), (XXVI), (XXVIII), (XXXIII), (XXXIV), (XXXV), (XXXVI) or (XXXVII) in a suitable carrier.
  • said suitable carrier is an environmentally friendly carrier comprising a lower alkanol selected from the group consisting of methanol, ethanol, n-propanol and i-propanol, water or a mixture thereof depending on the solubility of the phosphonate compound in the carrier.
  • the phosphonate antimicrobial coating can be applied onto a given surface preferably by dip coating, painting or with aerosol spraying with an about 1 to an about 20 mM solution of the phosphonate compound for a length of time so as to completely coat the surface.
  • the coating process may be repeated to apply additional layers of the phosphonate antimicrobial coating.
  • the stable and durable phosphonate antimicrobial coatings may be coated onto various material surfaces such as, but not limited to, metal oxides or metal alloys of aluminum, copper, iron, steel, titanium, zirconium and silicon (silica).
  • phosphonate antimicrobial coating strength and stability may be further enhanced by subjecting the uncoated surface to a pretreatment oxidation step known as passivation (Min, S. L., Smiley, K. J. & Gawalt, E. S. J. Am. Chem. Soc. 193-204 (2011)).
  • passivation creates a metal hydroxide layer that provides additional binding sites for the phosphonate compounds of the phosphonate antimicrobial coating to bind to.
  • Passivation can be accomplished known processes in the art such as thermal annealing (subjecting the uncoated surface to temperatures of about 100-140° C.
  • FIG. 1 shows the 1 H NMR of compound (1) of Referential Example 1
  • FIG. 2 shows the 13 C NMR of compound (1) of Referential Example 1
  • FIG. 3 shows the 31 P NMR of compound (1) of Referential Example 1
  • FIG. 4 shows the 1 H NMR of compound (2) of Example 1
  • FIG. 5 shows the 13 C NMR of compound (2) of Example 1
  • FIG. 6 shows the 31 P NMR of compound (2) of Example 1
  • FIG. 7 shows the 1 H NMR of compound (3) of Example 2
  • FIG. 8 shows the 13 C NMR of compound (3) of Example 2
  • FIG. 9 shows the 31 P NMR of compound (3) of Example 2
  • FIG. 10 shows the 1 H NMR of compound (4) of Example 3.
  • FIG. 11 shows the 13 C NMR of compound (4) of Example 3
  • FIG. 12 shows the 31 P NMR of compound (4) of Example 3
  • FIG. 13 shows the 1 H NMR of compound (5) of Example 4.
  • FIG. 14 shows the 13 C NMR of compound (5) of Example 4.
  • FIG. 15 shows the 31 P NMR of compound (5) of Example 4.
  • FIG. 16 shows the 1 H NMR of compound (6) of Example 5
  • FIG. 17 shows the 31 P NMR of compound (6) of Example 5
  • FIG. 18 shows the 1 H NMR of compound (7) of Example 6
  • FIG. 19 shows the 13 C NMR of compound (7) of Example 6
  • FIG. 20 shows the 31 P NMR of compound (7) of Example 6
  • FIG. 21 shows the 1 H NMR of compound (8) of Example 7
  • FIG. 22 shows the 13 C NMR of compound (8) of Example 7
  • FIG. 23 shows the 31 P NMR of compound (8) of Example 7
  • FIG. 24 shows the 1 H NMR of compound (9) of Example 8.
  • FIG. 25 shows the 13 C NMR of compound (9) of Example 8.
  • FIG. 26 shows the 31 P NMR of compound (9) of Example 8.
  • FIG. 27 shows the 1 H NMR of compound (10) of Example 9
  • FIG. 28 shows the 13 C NMR of compound (10) of Example 9
  • FIG. 29 shows the 1 H NMR of compound (11) of Example 10
  • FIG. 30 shows the 13 C NMR of compound (11) of Example 10
  • FIG. 31 shows the 1 H NMR of compound (12) of Example 11
  • FIG. 32 shows the 31 P NMR of compound (12) of Example 11
  • FIG. 33 shows the 1 H NMR of compound (13) of Example 12
  • FIG. 34 shows the 31 P NMR of compound (13) of Example 12
  • FIG. 35 shows the 1 H NMR of compound (14) of Example 13
  • FIG. 36 shows the 13 C NMR of compound (14) of Example 13
  • FIG. 37 shows the 31 P NMR of compound (14) of Example 13
  • FIG. 38 shows the 1 H NMR of compound (15) of Example 14
  • FIG. 39 shows the 13 C NMR of compound (15) of Example 14
  • FIG. 40 shows the 31 P NMR of compound (15) of Example 14
  • FIG. 41 shows the 1 H NMR of compound (16) of Example 15
  • FIG. 42 shows the 13 C NMR of compound (16) of Example 15
  • FIG. 43 shows the 31 P NMR of compound (16) of Example 15
  • FIG. 44 shows the 1 H NMR of compound (17) of Example 16
  • FIG. 45 shows the 13 C NMR of compound (17) of Example 16
  • FIG. 46 shows the 31 P NMR of compound (17) of Example 16
  • FIG. 47 shows the 1 H NMR of compound (18) of Example 17
  • FIG. 48 shows the 13 C NMR of compound (18) of Example 17
  • FIG. 49 shows the 1 H NMR of compound (19) of Example 18
  • FIG. 50 shows the 13 C NMR of compound (19) of Example 18
  • FIG. 51 shows the 1 H NMR of compound (20) of Example 19
  • FIG. 52 shows the 13 C NMR of compound (20) of Example 19
  • FIG. 53 shows the 31 P NMR of compound (20) of Example 19
  • FIG. 54 shows the 1 H NMR of compound (21) of Example 20
  • FIG. 55 shows the 13 C NMR of compound (21) of Example 20
  • FIG. 56 shows the 31 P NMR of compound (21) of Example 20
  • FIG. 57 shows the 1 H NMR of compound (30) of Example 21
  • FIG. 58 shows the 13 C NMR of compound (30) of Example 21
  • FIG. 59 shows the 1 H NMR of compound (31) of Example 22
  • FIG. 60 shows the 13 C NMR of compound (31) of Example 22
  • FIG. 61 shows the 31 P NMR of compound (31) of Example 22
  • FIG. 62 shows the 1 H NMR of compound (32) of Example 23
  • FIG. 63 shows the 13 C NMR of compound (32) of Example 23
  • FIG. 64 shows the 31 P NMR of compound (32) of Example 23
  • the present invention is directed to quaternary ammonium mono- and multidentate-phosphonate compounds, methods for manufacturing the compounds, compositions comprising said compounds and methods for treating surfaces and/or articles with the compounds to provide a durable, antimicrobial-treated article.
  • quaternary ammonium mono-phosphonate refers to quaternary ammonium compounds that have been substituted with a single phosphonate group O ⁇ P(OR) 2 where R is selected from methyl, ethyl, isopropyl, n-butyl or phenyl or hydrogen, preferably ethyl or hydrogen and even more preferably hydrogen, and the phosphonate group can be linked to the quaternary ammonium nitrogen centre by a one, two, three or four carbon atom chain, preferably a saturated chain.
  • quaternary ammonium multidentate-phosphonate refers to quaternary ammonium compound that have been substituted with two or more phosphonate groups O ⁇ P(OR) 2 where R is as above.
  • polar, aprotic solvent means a solvent that has a dipole moment but does not have an acidic hydrogen.
  • Non-limiting examples include acetonitrile, dimethylformamide, dimethylsulfoxide and dichloromethane.
  • polar, protic solvent means a solvent that has a dipole moment and has an acidic hydrogen.
  • Non-limiting examples including lower alkanols, carboxylic acids and water.
  • surface means any metallic or non-metallic article surface that is capable of forming phosphorus-oxygen bonds.
  • Non-limiting examples include steel, stainless steel, titanium, silica glass and clays.
  • neat means without the use of solvents, specifically directed to chemical reactions that do not involve the use of solvents.
  • quaternary ammonium mono- and bis-phosphonate compounds of the present invention can be prepared via one of several processes.
  • R 1 and R 2 are each independently a lower alkyl group preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl groups, m is 15, 16, 17, 18 or 19, n is 0, 1, 2, 3, 4, 5 or 6, and X is chloro, bromo or iodo, can be prepared by a process comprising the steps of (a) reacting a compound of formula (II)
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same and more preferably ethyl, with an alkyl halide of formula (III)
  • n and X are as above and Y is a halogen selected from chloro or bromo, more preferably bromo to give a compound of formula (IV)
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl groups, and m is 15, 16, 17, 18 or 19, to give a compound of formula (VI)
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl or hydrogen and even more preferably hydrogen
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl groups
  • m is 15, 16, 17, 18 or 19
  • n is 0, 1, 2, 3, 4, 5 or 6, o is 1, 2 or 3
  • Z is chloro, bromo or hydroxy, preferably bromo, is prepared by a process comprising the steps of (a) reacting at least two equivalents of compound of formula (IX)
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, with per equivalent of a compound of formula (X)
  • n 0, 1, 2, 3, 4, 5 or 6, and Z is selected from chloro, bromo or hydroxyl, preferably bromo, to give a compound of formula (XI)
  • R 1 and R 2 are independently lower alkyl groups preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, preferably methyl groups, and m is 15, 16, 17, 18 or 19, to give a compound of formula (VII) where R′, R 1 , R 2 , m, n, o and Z are as defined above.
  • the process for preparing the compound of formula (XI) can take place in a polar, aprotic solvent selected from but not limited to acetonitrile or dichloromethane, or neat, preferably neat, at a temperature from about ⁇ 5° C. to about 10° C. then warmed to about 90° C. to about 140° C. for about one hour.
  • the product of formula (XI) can be isolated by extraction and optionally purified, preferably by chromatography.
  • the process alternatively can take place in the presence of microwave radiation at a temperature of about 120° C. to about 140° C., preferably 130° C., for about five minutes.
  • the microwave radiation has a frequency of about 2500 MHz.
  • the process for preparing the compound of formula (VII) from the compound of formula (XI) can take place in a neat mixture of a compound of formula (XI) and a compound of formula (V) where R, R 1 , R 2 and Z are as defined above.
  • the process can take place in the absence of reaction solvent at a temperature of about 90° C. to about 110° C., preferably 100° C., for about one hour, or alternatively, in the presence of microwave radiation at a temperature of about 140° C. to about 160° C., preferably 150° C., for about one to three minutes, preferably about two minutes.
  • the microwave radiation has a frequency of about 2500 MHz.
  • the quaternary ammonium bis-phosphonate compound of formula (VII) is prepared by a process comprising the steps of
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl groups
  • n is 0, 1, 2, 3, 4, 5 or 6, o is 1, 2 or 3
  • Z is selected from chloro, bromo or hydroxyl, preferably bromo, with p-toluenesulfonyl chloride, trimethyl ammonium chloride, an organic amine, preferably triethylamine, in a polar, aprotic solvent selected from but not limited to acetonitrile, dimethylformamide or dichloromethane, preferably dichloromethane, (b) adding a compound R 1 R 2 NH where R 1 and R 2 are defined as above, in a polar, protic solvent selected from but not limited to methanol
  • step (a) can take place at a temperature of about 20° C. to about 30° C.
  • the process of step (b) can take place at a temperature of about 90° C. to about 115° C., preferably 100° C., and for a reaction time of about one hour.
  • the process of step (b) can take place in the presence of microwave radiation at a temperature of about 100° C. to about 120° C., preferably 110° C., for about five minutes.
  • the microwave radiation has a frequency of about 2500 MHz.
  • step (c) can take place neat at a temperature of about 90° C. to about 115° C. for about one hour or, alternatively, in the presence of microwave radiation at a temperature of about 140° C. to about 160° C., preferably 150° C., for about two minutes.
  • the microwave radiation has a frequency of about 2500 MHz.
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl or hydrogen and even more preferably hydrogen
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl groups
  • m is 15, 16, 17, 18 or 19
  • n is 0, 1, 2, 3, 4, 5 or 6
  • Z is selected from chloro or bromo, preferably bromo, is prepared by a process comprising the steps of (a) reacting a compound of formula (XV)
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, with a compound of formula (XVI)
  • n and R are as defined above, which is preferably not isolated and used in the next step, and (b) treating a compound of formula (XVII) with p-toluenesulfonic acid, methanesulfonyl chloride, triethylamine and R 1 R 2 NH where R 1 and R 2 are defined as above, and a compound of formula (XIII)
  • n 0, 1, 2, 3, 4, 5, or 6, with one equivalent of O ⁇ PH(OR) 2 where R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, in the presence of an alkali metal carbonate, preferably potassium carbonate, methanesulfonyl chloride and an organic amine base, preferably triethylamine, and further reacted with sodium hydride and a second equivalent of O ⁇ PH(OR) 2 to give a compound of formula (XIX)
  • step (c) can take place in a polar, aprotic solvent selected from but not limited to acetonitrile, tetrahydrofuran or dioxane, preferably acetonitrile or dioxane, at a temperature of about 25° C. to about 75° C., preferably 60° C.
  • a polar, aprotic solvent selected from but not limited to acetonitrile, tetrahydrofuran or dioxane, preferably acetonitrile or dioxane
  • n 0, 1, 2, 3, 4, 5, or 6 and R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, is prepared by a process comprising the step of reacting a compound of formula (XX)
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, more preferably methyl, is prepared by a process comprising reacting a compound of formula (XXIII)
  • n 0, 1, 2, 3, 4, 5 or 6, and R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, is prepared by a process comprising reacting a compound of formula (XXV)
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl or hydrogen and even more preferably hydrogen
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, preferably methyl groups
  • m is 15, 16, 17, 18 or 19
  • Z is selected from chloro or bromo, preferably bromo, is prepared by the process comprising the steps of (a) reacting oxalyl chloride with
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, and (b) reacting the compound of formula (XXVII) with a compound of formula (XIII) to give a compound of formula (XXVI).
  • step (a) can take place in a polar, aprotic solvent selected from but not limited to acetonitrile or dichloromethane, preferably dichloromethane, and the term chilled means a temperature from about ⁇ 5° C. to about 10° C., rising to about 20° C. to about 30° C. for about one hour.
  • a polar, aprotic solvent selected from but not limited to acetonitrile or dichloromethane, preferably dichloromethane
  • chilled means a temperature from about ⁇ 5° C. to about 10° C., rising to about 20° C. to about 30° C. for about one hour.
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl or hydrogen and even more preferably hydrogen
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, preferably methyl groups
  • m is 15, 16, 17, 18 or 19
  • Z is selected from chloro, bromo, iodo or mesyl, preferably bromo, is prepared by a process comprising the steps of (a) reacting a compound of (XXIX)
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, and n and Z are as defined above, in a polar, aprotic solvent to give a compound of formula (XXVIII).
  • the dansyl group is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, and n and Z are as defined above, in a polar, aprotic solvent to give a compound of formula (XXVIII).
  • UV fluorescing marker is used as a UV fluorescing marker to indicate the presence of the quaternary ammonium mono-phosphate compound after a compound of formula (XXVIII) has been applied to a surface.
  • quaternary ammonium multidentate tri- and tetra-substituted phosphonate compounds of formula (XXXIII), (XXXIV), (XXXV), (XXXVI) and (XXXVII) of formula (XXXIII), (XXXIV), (XXXV), (XXXVI) and (XXXVII)
  • R′ is independently hydrogen, methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl or hydrogen and even more preferably hydrogen
  • R 1 and R 2 are each independently a lower alkyl group, preferably saturated hydrocarbon chains being one, two or three carbon atoms in length, preferably methyl groups
  • m is 15, 16, 17, 18 or 19
  • Z is selected from chloro or bromo, preferably bromo.
  • Multidentate phosphonic acid antimicrobials may generally be prepared by introduction of the bis-, tris- or tetraphosphonate anchor prior to quaternization and dealkylation with trimethylbromosilane (TMBr).
  • TAMBP tetralkyl methylenebisphosphonate
  • monodeprotonation of TAMBP followed by mono alkylation leads to alpha (C—H) bisphosphonates whereas a second deprotonation/alkylation with dialkyl chlorophosphate provides trisphosphonates.
  • a second method for the synthesis of bisphosphonates is through Michael addition of dialkyl vinylphosphite to provide beta aminobisphosphonates. Further deprotonation and phosphorylation with dialkyl chlorophosphate provides tetraphosphonates.
  • the TRIS BOC scaffold containing three reactive groups may be turned into trisphosphonates via established synthetic routes used to prepare monophosphonates.
  • Two examples include the lewis acid-mediated Abrzov addition of trialkylphosphite three times to three reactive bromoacteylTRISBOC and the radical addition of dialkyl phosphite to terminal vinyl groups on TRISBOC.
  • General schemes for producing quaternary ammonium bis-, tris- and tetraphosphonate compounds are as follows:
  • R 1 and R 2 are independently lower alkyl groups, preferably methyl.
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, R 1 and R 2 are independently lower alkyl groups, preferably methyl and m is 15, 16, 17, 18 or 19.
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, R 1 and R 2 are independently lower alkyl groups, preferably methyl and m is 15, 16, 17, 18 or 19.
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, R 1 and R 2 are independently lower alkyl groups, preferably methyl and m is 15, 16, 17, 18 or 19.
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, and m is 15, 16, 17, 18 or 19.
  • R is independently methyl, ethyl, isopropyl, n-butyl or phenyl, preferably the same, more preferably ethyl, R 1 and R 2 are independently lower alkyl groups, preferably methyl and m is 15, 16, 17, 18 or 19.
  • the quaternary ammonium mono- and bis-phosphonate and multidentate tri- and tetra-substituted phosphonate compounds of the present invention may be used to antimicrobially treat hard surfaces.
  • a surface may be an inner surface and/or an outer surface.
  • a phosphonate antimicrobial coating composition for treating surfaces to give a stable and durable phosphonate antimicrobial coating surface treatment comprising any one of a compound of formulae (I), (VII), (XIV), (XXVI), (XXVIII), (XXXIII), (XXIV), (XXXV), (XXXVI) or (XXXVII) in a suitable carrier.
  • said suitable carrier is an environmentally friendly carrier comprising a lower alkanol selected from the group consisting of methanol, ethanol, n-propanol and i-propanol, water or a mixture thereof depending on the solubility of the phosphonate compound in the carrier.
  • the phosphonate antimicrobial coating may be applied onto a given surface preferably by dip coating, painting or with aerosol spraying with an about 1 to an about 20 mM solution of the phosphonate compound for a length of time so as to completely coat the surface. In one embodiment, the coating process may be repeated to apply additional layers of the phosphonate antimicrobial coating.
  • the stable and durable phosphonate antimicrobial coatings may be coated onto various material surfaces such as, but not limited to, metal oxides or metal alloys of aluminum, copper, iron, steel, titanium, zirconium and silicon (silica). Even more preferably, phosphonate antimicrobial coating strength and stability may be further enhanced by subjecting the uncoated surface to a pretreatment oxidation step known as passivation.
  • passivation creates a metal hydroxide layer that provides additional binding sites for the phosphonate compounds of the phosphonate antimicrobial coating to bind to. Passivation is accomplished by known processes in the art such as thermal annealing (subjecting the uncoated surface to temperatures of about 100-140° C. for about 18 hours) or reduced pressure annealing (subjecting the uncoated surface to pressures of about 0.05 to about 0.3 Torr, more preferably 0.1 Torr).
  • MPQ Monophosphonic Acid Quaternary Ammonium Antimicrobials
  • N-(3-(diethoxyphosphoryl)propyl)-N,N-dimethyloctadecan-1-ammonium bromide (0.2768 g, 0.46 mmol) was dissolved in anhydrous DCM (5 mL).
  • TMSBr (0.25 mL, 1.9 mmol, 4.0 eq.) through a rubber septum via syringe and the reaction was stirred at room temperature overnight. Completion of the reaction was followed by 31 P after which the reaction was quenched with EtOH (10 mL) and stirred for 1 h followed by addition of H 2 O (1 mL).
  • ⁇ -Amino Bisphosphonic Acid Antimicrobials ⁇ -ABPQ
  • the amine (1.350 g, 9.78 mmol) was added to the vial containing diethyl phosphite and cooled at 0-5° C. (ice bath). To the chilled, stirred solution was added formalin, dropwise (37%, 2.12 mL, 25.43 mmol, 2.6 eq.) over 10 min while maintaining the reaction temp under 10° C., then warming the mixture to room temperature for 30 min, and finally to 100° C. for 1 h.
  • diethylphosphite (2.86 g, 20.74 mmol, 2.0 eq.). The vial was placed on ice to cool. In a separate beaker, 3-aminopropyl-1-chloride hydrochloride (2.0 g, 11.4 mmol) was treated with NaOH ( ⁇ 12 N, 2 g in 5 mL) and stirred at 0° C. until a yellow oil appeared ( ⁇ 5 min). The mixture was then extracted without solvent, adding the upper yellow layer of the free base 3-aminopropyl-1-chloride to the vial containing diethyl phosphite cooled to 0-5° C.
  • the reaction was diluted with water (1 ⁇ 15 mL) and extracted with DCM (10 mL total), the aqueous layer was re-extracted with EtOAC (15 mL) and the combined organic layers were dried over MgSO 4 , filtered and evaporated to give a yellow oil.
  • the crude material was purified by flash chromatography on silica gel (20 g silica, 1.5 cm i.d) with gradient elution: 100% EtOAc (35 mL) then 5% MeOH:EtOAc (90 mL) to obtain the title compound as a yellow oil.
  • N-(4-Bromobutyl)-phthalimide 5 g, 17.7 mmol, 1.0 eq.
  • triethylphosphite 18.24 mL, 106.3 mmol, 6 eq.
  • the reaction was then cooled to room temperature and excess triethylphosphite was vacuum distilled using a shortpath distillation head attached to a Schlenk line. Once all of the excess triethylphosphite stopped distilling, the title compound was placed under high vacuum ( ⁇ 30 min) until it solidified.
  • Triethyl phosphite (18.77 mL, 109.5 mmol, 2.19 eq.) was then added dropwise with stirring. After 1 hr the mixture was concentrated under reduced pressure. The product was obtained as a yellow oil in 75.5% yield.
  • N-(3-(diethoxyphosphoryl)propyl)-N,N-dimethyloctadecan-1-ammonium bromide (0.35 g, 0.58 mmol) was dissolved in anhydrous DCM (5 mL).
  • TMSBr (0.23 mL, 1.76 mmol, 3.0 eq.) through a rubber septum via syringe and the reaction was stirred at room temperature overnight. Completion of the reaction was followed by 31 P after which the reaction was quenched with EtOH (10 mL) and stirred for 1 h followed by addition of H 2 O (1 mL).
  • Surface Pretreatment/Passivation modifies a metal surface by creating a metal hydroxide layer to provide more binding sites on the surface of the material to which phosphonic acid compounds can bind can be achieved by mechanical means such as sanding, cleaning, or degreasing; chemical means such as treatment with piranha solution (a 3:1 H 2 SO 4 /H 2 O 2 for about 10-30 minutes) or by activation by heating the surface (about 160° C. in air for about 1-2 hours). 2) Coating application by dip coating at room temperature to about 50° C.
  • Titanium foil sanded, rinsed with hot methanol, and stored at 160° C. in air, gives a surface coating of hydroxylated titanium dioxide. Aerosol sprayed (0.75 mM in THF), annealed 18 hrs at 120° C., immersion in (dry THF twice, for 5 min each). ⁇ 5016 Gawalt, Ellen S. 2001; ⁇ Similarly titanium disks were wet-ground (220-4000 grit silicon carbide paper and further polished with OPChem polishing cloths using OP-S colloidal silica suspension) followed by ultrasonication (deionized water to eliminate silica particles). Rinsed (acetone then ultrapure water) and dried for a few minutes in an oven (80° C.). (Lecollinet, G. et al. Self-Assembled Monolayers of Bisphosphonates: Influence of Side Chain Steric Hindrance. Langmuir 25, 7828-7835 (2009))
  • Stainless Steel Mechanically Polished (220, 400, 800, and 1200 grit silicon carbide paper followed by a 1 um diamond suspension). Ultrasonicated (MeOH, 15 min) or (DCM (10 min) then acetone (10 min)) and immersed (boiling MeOH to remove traces of organics and metallic dust), storage (120° C., oven). Dip coated (1 mM, dry tetrahydrofuran (THF)) and reduced pressure annealed (0.1 Torr). (Raman, A., Dubey, M., Gouzman, I. & Gawalt, E. S. Formation of Self-Assembled Monolayers of Alkylphosphonic Acid on the Native Oxide Surface of SS316L.
  • Silicon (100) wafer cleaning by sonication in acetone (15 min). oxidized (3:1, 30% H2O2:98% H2SO4 for 30 min), and rinsed (ultrapure water) and immediately dip coated (25 ⁇ M solution in THF until the solvent evaporated at room temperature). Thermal annealed (140 C 48 hrs). Three cycles of depositions with multiple rinsing and sonication in THF and methanol was used to produce a monolayer film. The films were stored in glass containers filled with nitrogen until they were characterized. (Hanson, E. L., Schwartz, J., Nickel, B., Koch, N. & Danisman, M. F. Bonding Self-Assembled, Compact Organophosphonate Monolayers to the Native Oxide Surface of Silicon. J. Am. Chem. Soc. 125, 16074-16080 (2003))
  • Non thermal annealing titanium samples dip coated (1 mM solution in acetone, 3 hrs) decant solvent and reduced pressure anneal (15 h at 50° C.). The samples were ultrasonically washed with acetone and then air-dried. (Lecollinet, G. et al. Self-Assembled Monolayers of Bisphosphonates: Influence of Side Chain Steric Hindrance. Langmuir 25, 7828-7835 (2009))
  • a one inch by inch TiO 2 square was sanded with 600 grain size sand paper, followed by an ethanol (EtOH) rinse. Samples were stored in a 120° C. oven prior to use. A 10 mM solution of compound (3) in EtOH was aerosol spayed onto the TiO 2 square, allowed to air dry and placed into 120° C. oven overnight to anneal the compound followed by an EtOH rinse. Spaying, annealing and rinsing were repeated two more times.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Materials Engineering (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
US15/938,151 2013-02-19 2014-02-12 Phosphorus functional antimicrobial coatings for metal surfaces Active USRE48510E1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US15/938,151 USRE48510E1 (en) 2013-02-19 2014-02-12 Phosphorus functional antimicrobial coatings for metal surfaces

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361766533P 2013-02-19 2013-02-19
US14/767,052 US9642369B2 (en) 2013-02-19 2014-02-12 Phosphorus functional antimicrobial coatings for metal surfaces
US15/938,151 USRE48510E1 (en) 2013-02-19 2014-02-12 Phosphorus functional antimicrobial coatings for metal surfaces
PCT/CA2014/000104 WO2014127451A1 (fr) 2013-02-19 2014-02-12 Revêtements antimicrobiens fonctionnels phosphorés pour surfaces métalliques

Publications (1)

Publication Number Publication Date
USRE48510E1 true USRE48510E1 (en) 2021-04-13

Family

ID=51390427

Family Applications (4)

Application Number Title Priority Date Filing Date
US15/938,151 Active USRE48510E1 (en) 2013-02-19 2014-02-12 Phosphorus functional antimicrobial coatings for metal surfaces
US14/767,052 Ceased US9642369B2 (en) 2013-02-19 2014-02-12 Phosphorus functional antimicrobial coatings for metal surfaces
US15/235,240 Active 2034-06-08 US10154668B2 (en) 2013-02-19 2016-08-12 Phosphonate functional antimicrobial coatings for metal surfaces
US16/215,955 Active US10327447B2 (en) 2013-02-19 2018-12-11 Phosphonate functional antimicrobial coatings for metal surfaces

Family Applications After (3)

Application Number Title Priority Date Filing Date
US14/767,052 Ceased US9642369B2 (en) 2013-02-19 2014-02-12 Phosphorus functional antimicrobial coatings for metal surfaces
US15/235,240 Active 2034-06-08 US10154668B2 (en) 2013-02-19 2016-08-12 Phosphonate functional antimicrobial coatings for metal surfaces
US16/215,955 Active US10327447B2 (en) 2013-02-19 2018-12-11 Phosphonate functional antimicrobial coatings for metal surfaces

Country Status (9)

Country Link
US (4) USRE48510E1 (fr)
EP (1) EP3105237B1 (fr)
CN (2) CN113150592A (fr)
AP (1) AP2015008631A0 (fr)
BR (1) BR112016018532B1 (fr)
CA (2) CA2923610A1 (fr)
IL (1) IL247209A0 (fr)
MX (1) MX2015010720A (fr)
WO (2) WO2014127451A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2884128C (fr) 2012-12-11 2016-02-09 Nano Safe Coatings Incorporated Revetements antimicrobiens a base d'ammonium quaternaire a terminaison benzophenone durcis par uv pour surfaces
CA2923610A1 (fr) 2013-02-19 2014-08-28 Nano Safe Coatings Incorporated (A Florida Corporation 3 P 14000024914) Revetements antimicrobiens fonctionnels phosphores pour surfaces metalliques
US9476754B2 (en) * 2013-02-28 2016-10-25 Electrolab, Inc. Method and kit for treatment of components utilized in a crude oil service operation
EP3341358B1 (fr) 2015-08-27 2023-03-08 Nano Safe Coatings Incorporated (a Florida Corporation 3 P 14000024914) Préparation d'agents antimicrobiens contenant du sulfonamide et compositions de traitement de substrat contenant des agents antimicrobiens contenant du sulfonamide
CN105887102A (zh) * 2016-04-25 2016-08-24 中国科学院兰州化学物理研究所 磷酸酯功能化的季铵盐缓蚀剂及其制备方法
US10072032B2 (en) * 2016-09-19 2018-09-11 Afton Chemical Corporation AminoBisPhosphonate antiwear additives
CN110312507B (zh) 2016-10-17 2021-11-23 奥索邦德公司 具有低聚或聚合抗微生物剂的表面
US10563042B2 (en) 2016-12-14 2020-02-18 Ecolab Usa Inc. Quaternary cationic polymers
CN107903286B (zh) * 2017-10-17 2020-08-04 山东泰和水处理科技股份有限公司 一种阻燃剂环己胺二甲叉膦酸及其制备方法
CN107827926B (zh) * 2017-10-31 2020-05-22 中国科学院兰州化学物理研究所 小分子功能化的表面活性剂及其制备方法和应用
US11427964B2 (en) 2018-06-12 2022-08-30 Ecolab Usa Inc. Quaternary cationic surfactants and polymers for use as release and coating modifying agents in creping and tissue papers
EP3898733A2 (fr) 2018-12-19 2021-10-27 3M Innovative Properties Company Copolymères zwittérioniques, compositions de revêtement, articles et procédés de revêtement
EP4140304A1 (fr) * 2019-05-31 2023-03-01 Rolex S.A. Composition pour imprégner un substrat, en particulier un bracelet de montre
KR20210031159A (ko) * 2019-09-11 2021-03-19 주식회사 엘지화학 리튬 이차전지용 비수전해액 및 이를 포함하는 리튬 이차전지
CN113336672A (zh) * 2021-05-21 2021-09-03 上海应用技术大学 利用威廉姆森成醚反应合成三臂化含端烯/炔烃化合物的方法
CN114405542B (zh) * 2022-01-28 2024-02-27 万华化学集团股份有限公司 一种负载型磁性纳米金属催化剂及其制备方法和应用

Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2774786A (en) 1953-08-10 1956-12-18 Gen Mills Inc Phosphono-ammonium surface active agents
US3454677A (en) 1966-03-07 1969-07-08 Eastman Kodak Co Inner salts of aminophosphonates and preparation thereof
US3507937A (en) 1966-10-04 1970-04-21 Procter & Gamble Reversed zwitterionic phosphorus compounds
US3925453A (en) 1974-01-25 1975-12-09 Monsanto Co Quaternary aminoalkylene phosphonic acids and method of preparation
US4208401A (en) 1977-08-19 1980-06-17 Colgate-Palmolive Company Quaternary ammonium alkylene diphosphonate anti-calculus agents
US4420399A (en) 1978-04-20 1983-12-13 Petrolite Corporation Quaternary aminomethyl phosphonates as scale inhibitors
EP0279475A2 (fr) 1987-02-17 1988-08-24 Ward Blenkinsop And Company Limited Dérivés de benzophénone
US5753634A (en) 1992-05-29 1998-05-19 The Procter & Gamble Company Quaternary nitrogen containing phosphonate compounds, pharmaceutical compostions, and methods for treating abnormal calcium and phosphate metabolism
CA2318733A1 (fr) 1998-01-27 1999-07-29 International Coatings Limited Peintures antisallissures
WO2001025171A1 (fr) 1999-10-05 2001-04-12 Glaxo Group Limited Constructions chimiques
CA2636052A1 (fr) 2006-02-23 2007-09-07 E.I. Dupont De Nemours And Company Compositions de revetement antimicrobiennes amovibles et procedes d'utilisation de celles-ci
US20080220037A1 (en) 2004-11-22 2008-09-11 Surfactis Technologies Biphosphonic Compounds For Preventing Or Limiting Fixing Of Macromolecules, Microorganisms And Biofilm On Solid, In Particular Metal Or Mineral Surfaces
US7435841B2 (en) * 2004-03-10 2008-10-14 Rhodia Inc. Preparation of halohydrocarbyl phosphonic acid diesters
US20100121075A1 (en) 2007-02-16 2010-05-13 Centre National De La Recherche Scientifique-Cnrs- Alkyl h-phosphonates of n,n'-dialkylimidazoliums and of quaternary ammoniums and uses thereof
EP2282209A1 (fr) 2008-04-30 2011-02-09 Sumitomo Bakelite Company Limited Procédé de marquage de chaînes glucidiques
WO2012160187A1 (fr) * 2011-05-26 2012-11-29 Jado Technologies Gmbh Dérivés d'acide sulfonique, d'acide phosphonique et d'acide carbonique contenant un groupe amino ou ammonium et leur utilisation médicale
CA2858596A1 (fr) 2011-12-14 2013-06-20 Semprus Biosciences Corp. Procede a resistance ionique elevee pour la modification de lentille de contact
WO2014127451A1 (fr) 2013-02-19 2014-08-28 Nano Safe Coatings Incorporated (A Florida Corporation 3 P 14000024914) Revêtements antimicrobiens fonctionnels phosphorés pour surfaces métalliques

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6994890B2 (en) * 2003-10-31 2006-02-07 Resource Development L.L.C. Cleaning and multifunctional coating composition containing an organosilane quaternary compound and hydrogen peroxide
US8361779B2 (en) * 2010-11-24 2013-01-29 Sachem, Inc. Buffer compounds

Patent Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2774786A (en) 1953-08-10 1956-12-18 Gen Mills Inc Phosphono-ammonium surface active agents
US3454677A (en) 1966-03-07 1969-07-08 Eastman Kodak Co Inner salts of aminophosphonates and preparation thereof
US3507937A (en) 1966-10-04 1970-04-21 Procter & Gamble Reversed zwitterionic phosphorus compounds
US3925453A (en) 1974-01-25 1975-12-09 Monsanto Co Quaternary aminoalkylene phosphonic acids and method of preparation
US4208401A (en) 1977-08-19 1980-06-17 Colgate-Palmolive Company Quaternary ammonium alkylene diphosphonate anti-calculus agents
US4420399A (en) 1978-04-20 1983-12-13 Petrolite Corporation Quaternary aminomethyl phosphonates as scale inhibitors
EP0279475A2 (fr) 1987-02-17 1988-08-24 Ward Blenkinsop And Company Limited Dérivés de benzophénone
US5753634A (en) 1992-05-29 1998-05-19 The Procter & Gamble Company Quaternary nitrogen containing phosphonate compounds, pharmaceutical compostions, and methods for treating abnormal calcium and phosphate metabolism
CA2318733A1 (fr) 1998-01-27 1999-07-29 International Coatings Limited Peintures antisallissures
WO2001025171A1 (fr) 1999-10-05 2001-04-12 Glaxo Group Limited Constructions chimiques
US7435841B2 (en) * 2004-03-10 2008-10-14 Rhodia Inc. Preparation of halohydrocarbyl phosphonic acid diesters
US20080220037A1 (en) 2004-11-22 2008-09-11 Surfactis Technologies Biphosphonic Compounds For Preventing Or Limiting Fixing Of Macromolecules, Microorganisms And Biofilm On Solid, In Particular Metal Or Mineral Surfaces
CA2636052A1 (fr) 2006-02-23 2007-09-07 E.I. Dupont De Nemours And Company Compositions de revetement antimicrobiennes amovibles et procedes d'utilisation de celles-ci
US20100121075A1 (en) 2007-02-16 2010-05-13 Centre National De La Recherche Scientifique-Cnrs- Alkyl h-phosphonates of n,n'-dialkylimidazoliums and of quaternary ammoniums and uses thereof
EP2282209A1 (fr) 2008-04-30 2011-02-09 Sumitomo Bakelite Company Limited Procédé de marquage de chaînes glucidiques
WO2012160187A1 (fr) * 2011-05-26 2012-11-29 Jado Technologies Gmbh Dérivés d'acide sulfonique, d'acide phosphonique et d'acide carbonique contenant un groupe amino ou ammonium et leur utilisation médicale
CA2858596A1 (fr) 2011-12-14 2013-06-20 Semprus Biosciences Corp. Procede a resistance ionique elevee pour la modification de lentille de contact
WO2014127451A1 (fr) 2013-02-19 2014-08-28 Nano Safe Coatings Incorporated (A Florida Corporation 3 P 14000024914) Revêtements antimicrobiens fonctionnels phosphorés pour surfaces métalliques

Non-Patent Citations (15)

* Cited by examiner, † Cited by third party
Title
Banerjee, Indrani, et al., "Antifiouling Coatings: Recent Developments in the Design of Surfaces that Prevent Fouling by Proteins, Bacteria, and Marine Organisms", Adv. Mater 2011, 23, 690-718.
Boczula, Dorota, et al., "Structural and vibrational characteristics of amphiphillic phosphonate salts", Journal of Molecular Structure 1007 (2012) 220-226.
BR112016018532 English language published May 8, 2018, Abstract of corresponding document WO2014127451(a1).
Brodersen, Nicolai, et al., "Synthesis of novel amphipilic conjugates with a biological recognition function for developing targeted triggered liposomal delivery systems", Tetrahedron 67 (2011) 7763-7774.
Huang, Xiao-Jun, et al., "Surface Modification of Polyacrylonitrile-Based Membranes by Chemical Reactions to Generate Phospholipid Moieties", Langmuir 2005, 21, 2941-2947.
International Search Report for PCT/CA2014/000104 dated Jun. 12, 2014.
International Search Report for PCT/CA2014/050796 dated Dec. 23, 2014.
Karimi, Ali, et al., "Effect of chain length and electrical charge on properties of ammonium-bearing bisphosphonate-coated superparamagnetic iron oxide nanoparticles formulation and physicochemical studies", J. Nanopart Res (2010) 12:1239-1248.
Kugel, Alex, et al., "Antimicrobial coatings produced by "tethering" biocides to the coating matrix, A comprehensive review", Progress in Organic Coatings 72 (2011) 222-252.
Page, Kristopher, et al., "Antimicrobial surfaces and their potential in reducing the role of the inanimate environment in the incidence of hospital-acquired infections", J. Mater. Chem., 2009, 19, 3819-3831.
Porosa, LM. et al. "Rapid Microwave-Assisted Synthesis of γ-Phosphonic Acid Quaternary Ammonium Antimicrobials for Biomedical Applications", Current Microwave Chemistry, vol. 2, No. 1, 2015, pp. 69-82(14).
Porosa, Lukasz M., et al., "Synthesis, structures and properties of self-assembling quatemary ammonium dansyl fluorescent tags for porous and non-porous surfaces", J. Mater. Chem. B, 2014, 2, 1509-1520.
Portet, David, et al., "Comparative Biodistribution of Thin-Coated Iron Oxide Nanoparticles TCION: Effect of Different Bisphosphonate Coatings", Drug Development Research 54: 173-181 (2001).
Queffelec, Clemence, et al., "Surface Modification Using Phosphonic Acids and Esters", Chem. Rev. 2012, 112, 3777-3807.
Takashi, Jin, "A New Fluorometric Method for the Deection of the Neurotransmitter Acetylcholine in Water Using Dansylcholine Complex with p-Sulfonated Calix[8]arene", Journal of inclusion Phenomena and Macrocyclic Chemistry 45: 196-201, 2003.

Also Published As

Publication number Publication date
BR112016018532B1 (pt) 2021-09-14
IL247209A0 (en) 2016-09-29
US10154668B2 (en) 2018-12-18
US20160374345A1 (en) 2016-12-29
WO2014127451A1 (fr) 2014-08-28
US20160066579A1 (en) 2016-03-10
AP2015008631A0 (en) 2015-08-31
US9642369B2 (en) 2017-05-09
EP3105237A4 (fr) 2017-08-16
MX2015010720A (es) 2016-07-11
EP3105237A1 (fr) 2016-12-21
WO2015120531A1 (fr) 2015-08-20
EP3105237B1 (fr) 2021-10-06
CA2900282A1 (fr) 2014-08-28
US10327447B2 (en) 2019-06-25
US20190116798A1 (en) 2019-04-25
CA2923610A1 (fr) 2014-08-28
CN106232610A (zh) 2016-12-14
CN113150592A (zh) 2021-07-23
BR112016018532A2 (pt) 2018-05-08
CA2900282C (fr) 2016-07-26

Similar Documents

Publication Publication Date Title
USRE48510E1 (en) Phosphorus functional antimicrobial coatings for metal surfaces
US9994721B2 (en) Photoactivatable crosslinker
Queffélec et al. Surface modification using phosphonic acids and esters
US9206205B2 (en) Hydrolysable and polymerizable silanes with adjustable spatial distribution of the functional groups, and use thereof
JP2013523751A5 (fr)
Breucker et al. Phosphonic acid-functionalized polyurethane dispersions with improved adhesion properties
AU2021282689A1 (en) Graftable biocidal linkers and polymers and uses thereof
US20210017400A1 (en) Molecularly Well-defined Antibiofouling and Polyionic Coatings
US8586758B2 (en) Preparation of an inorganic substrate having antimicrobial properties
Porosa et al. Phosphonate functional antimicrobial coatings for metal surfaces
US10889726B2 (en) Polymer having antimicrobial and/or antifouling properties
US9657042B2 (en) Silane compounds and use of same for functionalizing solid supports and immobilizing biological molecules on these supports
JP2013537883A (ja) 表面機能化のための柔軟な基本構造を有する三脚型カテコール誘導体の合成
M Porosa et al. Rapid Microwave-Assisted Synthesis of γ-Phosphonic Acid Quaternary Ammonium Antimicrobials for Biomedical Applications
JP2013540729A (ja) 表面機能化のための柔軟な基本構造を有する三脚型ビスホスホネート誘導体の合成
US20130217916A1 (en) Synthesis of Tripodal Bisphosphonate Derivatives Having an Adamantyl Basic Framework for Functionalizing Surfaces
JP2016210778A (ja) 化合物、表面処理剤、及び表面処理方法

Legal Events

Date Code Title Description
FEPP Fee payment procedure

Free format text: ENTITY STATUS SET TO UNDISCOUNTED (ORIGINAL EVENT CODE: BIG.); ENTITY STATUS OF PATENT OWNER: SMALL ENTITY

FEPP Fee payment procedure

Free format text: ENTITY STATUS SET TO SMALL (ORIGINAL EVENT CODE: SMAL); ENTITY STATUS OF PATENT OWNER: SMALL ENTITY

AS Assignment

Owner name: BIOSHIELD TECHNOLOGIES CANADA LIMITED, CANADA

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:POROSA, LUKASZ;WOLFAARDT, GIDEON;FOUCHER, DANIEL;SIGNING DATES FROM 20130313 TO 20130413;REEL/FRAME:055281/0367

Owner name: NANO SAFE COATINGS INCORPORATED, FLORIDA

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:BIOSHIELD TECHNOLOGIES CANADA LIMITED;REEL/FRAME:055283/0068

Effective date: 20140328