US3930736A - Aperture tube with attached thief - Google Patents

Aperture tube with attached thief Download PDF

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Publication number
US3930736A
US3930736A US05/484,581 US48458174A US3930736A US 3930736 A US3930736 A US 3930736A US 48458174 A US48458174 A US 48458174A US 3930736 A US3930736 A US 3930736A
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US
United States
Prior art keywords
thief
conduit
aperture tube
aperture
tube
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US05/484,581
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English (en)
Inventor
Wallace Coulter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Electronics Inc
Original Assignee
Coulter Electronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coulter Electronics Inc filed Critical Coulter Electronics Inc
Priority to US05/484,581 priority Critical patent/US3930736A/en
Priority to JP50080159A priority patent/JPS5125171A/ja
Priority to FR7520499A priority patent/FR2277383A1/fr
Application granted granted Critical
Publication of US3930736A publication Critical patent/US3930736A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle

Definitions

  • an electronic particle counting device operating on the Coulter principle is combined with an optical hemoglobinometer such that at some step in the sequence of events of the operation of the counting device, the operation of the optical hemoglobinometer is started.
  • the same sample suspension that is utilized in the counting device is also utilized in the hemoglobinometer by means of a fluid connection from the counting device to the hemoglobinometer so that there is no need to handle the sample twice.
  • the electronic particle counting device includes an aperture tube such as is commonly known in the art.
  • the fluid connection to the hemoglobinometer is, in the above identified patent, by means of a thief, hereinafter termed a thief conduit.
  • a liquid suspension of particles is placed in a beaker which is brought into contact with the aperture tube and thief conduit.
  • the beaker with remaining liquid suspension is removed and another beaker with a new liquid suspension to be analyzed is brought into contact with the aperture tube and thief conduit so that the analyzing cycle can be repeated.
  • the thief conduit draws suspension from the same height in the suspension as the aperture in the aperture tube, it can be assumed that the particles being counted are substantially the same as those being passed through the optical hemoglobinometer. As a result, the probability of a correct analysis is statistically increased. Much time and effort must be spent by a technician to properly align the aperture tube and thief conduit so that when liquid suspension is brought into contact with the aperture tube and thief conduit, they will be at the same height in the liquid suspension. Furthermore, the apparatus must be regularly checked to ensure continued proper alignment, causing a continued expenditure of effort on unproductive activity.
  • the draw of fluid by the thief conduit can disturb the fluid adjacent the aperture in the aperture tube. if the fluid is disturbed the particle count can be adversely affected.
  • the technician, in setting up the equipment, must exercise great care to ensure that the aperture tube and thief conduit are positioned so as to reduce the possibility of one affecting the operation of the other. Again, there is a continued expenditure of time and effort on what can be considered unproductive activity.
  • an apparatus for use in a particle measuring device where liquid suspensions of particles to be measured are brought into contact with the apparatus.
  • the liquid suspensions can adhere to the apparatus and carry over from one suspension to another causing contamination.
  • the apparatus includes an aperture tube and a thief conduit which are attached to one another and form a unitary structure.
  • This unitary structure has its exterior surface constructed and arranged to minimize carry over from one liquid suspension to another.
  • the aperture tube and thief conduit outer surfaces are attached along a line forming a junction between the two.
  • the exterior surfaces of the aperture tube and thief conduit form a V shaped depression on each side of this junction.
  • the V shaped depression is filled with material so as to form a substantially smooth exterior surface, thus minimizing the possibility of carry over.
  • the thief is elongate and is positioned in the aperture tube.
  • the opposite ends of the thief conduit open through the walls of the aperture tube to the exterior of the aperture tube.
  • FIG. 1 is a section view showing one embodiment of the aperture tube and attached thief of this invention
  • FIG. 2 is a cross section of the aperture tube and attached thief along the lines 1--1 of FIG. 1;
  • FIG. 3 is a section view of another embodiment of the aperture tube and attached thief of this invention.
  • FIGS. 1 and 2 there is shown an aperture tube with attached thief conduit 10.
  • the aperture tube with attached thief conduit 10 includes an elongate cylindrical aperture tube 12 with an aperture 14 formed in the side wall thereof.
  • Aperture tube 12 is of the type well known in the art, reference being made to U.S. Pat. No. 2,656,508 for a more detailed description of the aperture tube itself. It is sufficient to further identify only bottom end 16, which is closed, and open top end 18 of aperture tube 12.
  • a thief conduit 20 has a bore 22 passing therethrough, and an open top and bottom end 24 and 26, respectively. Open end 24 attaches to an optical hemoglobinometer such as is shown in the above noted U.S. Pat. No. 3,743,424.
  • Thief conduit 20 and aperture tube 12 are attached to one another along a line forming a junction and identified generally at 28 in FIG. 2.
  • the side walls of aperture tube 12 and conduit thief 20, on each side of junction 28, form V shaped depressions 30.
  • material 36 is formed in V shaped depressions 30, filling the depressions and resulting in a smooth circular or eliptical external surface.
  • the external surface is constructed and arranged to minimize carry over of liquid suspension from one suspension to another.
  • the external surface area is minimized thus minimizing the contact area with liquid suspension 32 and the carry over which can result from the adhesion to the surface area itself.
  • aperture tube 12, thief conduit 20 and material 36 must be impervious to deterioration when brought into contact with different types of liquid suspension.
  • aperture tube 12, conduit tube 20 and material 36 are glass.
  • Material 36 will be formed by the V shaped depressions as the aperture tube 12 and the conduit 20 are formed resulting in a unitary structure.
  • aperture tube 12 and thief conduit 20 are shown as having a circular configuration in FIG. 2, it is to be understood that they need not be perfectly circular. In fact, it may be found preferable that they not be circular but rather ellipsoidal so that when formed together with material 36 filling the V shaped depressions formed by the junction, the entire outer surface will be circular in shape when viewed from the top end as in FIG. 2. A circular exterior surface will provide the minimum surface area for contact with liquid suspension 32.
  • thief conduit 20 is secured to aperture tube 12 such that bottom end 26 is at substantially the same height in liquid suspension 32 as aperture 14. This allows liquid suspension to be drawn into bore 22 of thief conduit 20 which is substantially the same as the liquid suspension entering aperture 14, so that the particles counted will be substantially the same as those being passed through the optical hemoglobinometer attached to top end 24 of thief conduit 20.
  • thief conduit 20 is secured to aperture tube 12 along a line substantially radially opposite to aperture 14. By positioning thief conduit 20 substantially radially opposite to aperture 14 the fluid drawn into bottom end 26 of thief conduit 20 will not disturb the liquid suspension surrounding aperture 14 so that the particle count, based upon particles passing through aperture 14 will not be detrimentally affected.
  • FIG. 3 another embodiment of the aperture tube and attached thief conduit of this invention is shown generally at 10.
  • An elongate cylindrical aperture tube 12 is again shown having an aperture 14 positioned in the side wall thereof.
  • Aperture tube 12 has a closed bottom end 16 and open top end 18 as shown in FIGS. 1 and 2.
  • a thief conduit 40 having a bore 42 passing therethrough, is positioned in the interior 41 of aperture tube 12.
  • the top end 44 and the bottom end 46 extend through the side wall of aperture tube 12.
  • end 46 of thief conduit 40 terminates at an opening 48 in the side wall of aperture tube 12 with the axis of bore 42 axially aligned with opening 48.
  • Top end 44 of thief conduit 40 passes through the side wall of aperture tube 12 at a point 50 and terminates at a point 52 beyond side wall of aperture tube 50. This allows end 44 to be attached to the optical hemoglobinometer such as shown in the above noted U.S. Pat. No. 3,743,424.
  • connection between thief conduit 40 and opening 48 in the side wall of aperture tube 12 is fluid tight so that liquid suspension entering opening 48 will only pass through bore 42 and will not enter interior 41 of aperture tube 12.
  • the connection between thief conduit 40 and aperture tube 12 at 50 is also fluid tight preventing any liquid suspension in the interior 41 of aperture tube 12 from escaping.
  • aperture tube 12 and thief conduit 40 are glass.
  • the opening 48 in the side wall of aperture tube 12 is substantially radially opposite to aperture 14 and is located at substantially the same height below open end 18 of aperture tube 12 as aperture 14, both for the same reasons noted above with respect to the embodiment shown in FIGS. 1 and 2.
  • the only surface area to which liquid suspension could adhere is the exterior surface of aperture tube 12 itself. Any suspension which adheres to the side walls of thief conduit 40 would not affect or contaminate a subsequent suspension into which aperture tube 12 is placed because the suspension adhering to thief conduit 40 would only contaminate the new suspension after the suspension has passed through aperture 14 and has been counted. Once the counting has occurred, contamination is of no consequence. Furthermore, positioning thief conduit 20 internal to aperture tube 12 minimizes the external surface area and eliminates unusual external contours so as to minimize the possibility of carry over from one liquid suspension to another.
  • an aperture tube with attached thief conduit has been provided for use in a particle measuring device.
  • the unitary structure minimizes external surface area, thus minimizing the possibility of carry over of liquid suspension.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
US05/484,581 1974-07-01 1974-07-01 Aperture tube with attached thief Expired - Lifetime US3930736A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US05/484,581 US3930736A (en) 1974-07-01 1974-07-01 Aperture tube with attached thief
JP50080159A JPS5125171A (en) 1974-07-01 1975-06-30 Ryushisokuteisochokansochi
FR7520499A FR2277383A1 (fr) 1974-07-01 1975-06-30 Tube a ouverture avec pipette attachee

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US05/484,581 US3930736A (en) 1974-07-01 1974-07-01 Aperture tube with attached thief

Publications (1)

Publication Number Publication Date
US3930736A true US3930736A (en) 1976-01-06

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ID=23924739

Family Applications (1)

Application Number Title Priority Date Filing Date
US05/484,581 Expired - Lifetime US3930736A (en) 1974-07-01 1974-07-01 Aperture tube with attached thief

Country Status (3)

Country Link
US (1) US3930736A (Direct)
JP (1) JPS5125171A (Direct)
FR (1) FR2277383A1 (Direct)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4070263A (en) * 1975-09-16 1978-01-24 Degremont Apparatus for the measurement of the mobility of colloids in an electric field
WO1989004961A1 (en) * 1987-11-13 1989-06-01 Techne Corporation Hematology cell counting apparatus
EP0339268A3 (en) * 1988-04-29 1991-02-27 Becton, Dickinson and Company Backflow isolator and capture system
US5094818A (en) * 1989-05-04 1992-03-10 Exact Science, Inc. Self-filling anti-siphon flow system for particle analysis
GB2337598A (en) * 1996-11-20 1999-11-24 Microbial Systems Ltd Particle sizing apparatus
GB2319618B (en) * 1996-11-20 2000-08-23 Microbial Systems Ltd Particle sizing apparatus
US6418802B1 (en) 1996-11-21 2002-07-16 Michael Anthony Wood Particle sizing apparatus and method of use thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3743424A (en) * 1970-11-19 1973-07-03 Coulter Electronics Combined electronic and optical method and apparatus for analyzing liquid samples
US3746977A (en) * 1971-08-23 1973-07-17 Coulter Electronics Aperture tube assembly for a particle study apparatus
US3746976A (en) * 1971-04-07 1973-07-17 Coulter Electronics Self-cleaning aperture tube for coulter study apparatus

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3743424A (en) * 1970-11-19 1973-07-03 Coulter Electronics Combined electronic and optical method and apparatus for analyzing liquid samples
US3746976A (en) * 1971-04-07 1973-07-17 Coulter Electronics Self-cleaning aperture tube for coulter study apparatus
US3746977A (en) * 1971-08-23 1973-07-17 Coulter Electronics Aperture tube assembly for a particle study apparatus

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4070263A (en) * 1975-09-16 1978-01-24 Degremont Apparatus for the measurement of the mobility of colloids in an electric field
WO1989004961A1 (en) * 1987-11-13 1989-06-01 Techne Corporation Hematology cell counting apparatus
EP0339268A3 (en) * 1988-04-29 1991-02-27 Becton, Dickinson and Company Backflow isolator and capture system
US5094818A (en) * 1989-05-04 1992-03-10 Exact Science, Inc. Self-filling anti-siphon flow system for particle analysis
GB2337598A (en) * 1996-11-20 1999-11-24 Microbial Systems Ltd Particle sizing apparatus
GB2337598B (en) * 1996-11-20 2000-08-16 Microbial Systems Ltd Particle sizing apparatus
GB2319618B (en) * 1996-11-20 2000-08-23 Microbial Systems Ltd Particle sizing apparatus
US6389912B1 (en) 1996-11-20 2002-05-21 Michael Anthony Wood Particle sizing apparatus and method of use thereof
US6418802B1 (en) 1996-11-21 2002-07-16 Michael Anthony Wood Particle sizing apparatus and method of use thereof

Also Published As

Publication number Publication date
FR2277383B1 (Direct) 1977-07-22
FR2277383A1 (fr) 1976-01-30
JPS5125171A (en) 1976-03-01

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