Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
  • G01N33/521—Single-layer analytical elements
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10S435/00—Chemistry: molecular biology and microbiology
  • Y10S435/805—Test papers
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
  • Y10T436/145555—Hetero-N
  • Y10T436/146666—Bile pigment
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/20—Oxygen containing
  • Y10T436/200833—Carbonyl, ether, aldehyde or ketone containing

Definitions

• the present invention is concerned with diagnostic agents for the detection of components in liquids, especially in body fluids, comprising an absorbent carrier impregnated with reagents.
• test strips impregnated with suitable reagents have been in use for a long time for detecting components of liquids. pH indicator papers are used very widely but other reagent papers are also employed. In recent years, test papers have achieved great importance for the detection of glucose, protein, nitrite and the like in body fluids, for example in urine and blood, because they enable the physician to carry out a rapid and simple diagnosis of metabolic disturbances outside the laboratory.
• test reagents include, for example, strongly alkaline, strongly acidic and oxidizing substances and especially reagent mixtures with a high salt content.
• Tests which are especially important for medical diagnosis but which cannot be carried out or cannot be carried out satisfactorily with the use of test strips include, for example, Legals test for ketonic bodies and Ehrlichs test of pyrrole bodies. Legals test requires, for a satisfactory functioning, a high concentration of a strongly alkaline buffer in the reaction solution and Ehrlichs test requires a high concentration of a weakly acidic compound, for example, oxalic acid or potassium bisulfate.
• the present invention provides diagnostic agents comprising an absorbent carrier which consists entirely or preponderantly of polyamides. Only these fleeces or felts based on polyamides provide the final test strips with a sufficient degree of stability, without destroying the reactivity and sensitivity of the test reagents.
• polyamides differ in this manner from other synthetic resins, for example from polyesters and polyvinyl chlorides.
• the difference is extremely surprising because the reagents are also not embedded or incorporated into the polyamide fibers.
• polyamides to be used according to the present invention there can be employed not only those of the nylon type, made from dicarboxylic acids and diamines, but also those of the perlon type, made from w-aminocarboxylic acids.
• polyester fibers As mixture components in mixed felts and fleeces, it is especially preferred to use polyester fibers but fibers of other synthetic resins, for example of polyvinyl chloride and the like, can also be admixed in amounts of up to 50%.
• the felts and fleeces can be produced not only be wet-depositing but also by dry-depositing, the fibers can be oriented or lie at random and they can be connected thermally or by binding agents or they can be needled.
• the felt or fleece selecteddepends essentially upon the nature of the reagents to be used for the impregnation thereof. From the large number of commercially available felts and fleeces the best one can easily be determined by a few simple preliminary experiments.
• the thickness and weight per unit area of the felt or fleece used can be varied. However, very thin or light felts and fleeces take up very little reagent and thus have a poorer reactivity. Thick or voluminous felts and fleeces, on the other hand, take up large amounts of reagents and it is thus more difficult to work them up.
• the most suitable felt or fleece for any particular case can be easily determined by a few simple preliminary experiments.
• the felt or fleece can be impregnated in conventional manner. However, in order to improve wettability, it is sometimes desirable either to add a wetting agent to the impregnation solution or first to impregnate the felt or fleece with a wetting agent.
• the impregnated felt or fleece is then dried in the usual manner. If desired, it can be cut up into narrow strips and used directly or, as still smaller pieces, can either be stuck on to a handle of synthetic resin or, according to German Patent Specification No. 1,546,307, can be sealed between synthetic resin films or, according to German Patent Specification No. P 21 18 455.4, can be sealed between a synthetic resin film and a synthetic resin mesh.
• Ketonic Body Test The materials set out in the following Table 2 were impregnated with Solution I, dried, impregnated with Solution II and again dried.
• Solution l trisodium phosphate dodecahydrate disodium hydrogen phosphate dihydrate glycocoll distilled water
• Solution ll sodium nitroferricyanide dihydrate copolymer (50% solution in ethanol) organic phosphate ester of anionic 38.0 ml 18.5 ml ad 100.0 ml
• Solution 1 tetrasodium ethylenediaminetetraacetate 38.5 g glycocoll 18.7 g distilled water ad 100.0 ml
• Table 3 also shows the mechanical properties and the reactions of the test strips with urine which contains increasing amounts of acetoacetate or of acetone.
• polyester fleece l0 The following Table 4 gives the properties and description of the various carrier materials used in the above Examples:
• fleece (sample) denberg lactam and transverse- M.W. about ly laid, therm- 20,000 ally strengthened 7 polyamide FT 21 14 C. Freue-caprolongitudinally 0.25 80 fleece denberg lactam and transversely M.W. about laid, thermally 20,000 strengthened 8 polyester- Suprotex Kalle terephthalic longitudinally 1.5 300 polyamide acid-ethylene and transversely fleece glycol M.W. laid, thermally (1:1) about 18,000 strengthened,
• Diagnostic device for the detection of components contained in liquids, which diagnostic device comprises an absorbent carrier comprising felt or fleece preponderantly consisting of polyamide fibers, wherein said carrier is impregnated with appropriate reagents.
• said other fiber is at least one member of the group consisting of polyester fibers, polyvinylchloride fibers and cellulose fibers.
• Diagnostic device as claimed in claim 1 wherein the reagents are strongly alkaline phosphate buffer, sodium nitroferricyanide and glycocoll, for carrying out Legals test for ketonic bodies.
• Diagnostic device as claimed in claim 1 wherein the reagents are potassium bisulfate and pdimethylaminobenzaldehyde, for carrying out Ehrlichs test for urobilinogen.
• said absorbent carrier is a mixed felt or fleece also containing polyester fibers.

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Hematology (AREA)
• Immunology (AREA)
• Engineering & Computer Science (AREA)
• Urology & Nephrology (AREA)
• Molecular Biology (AREA)
• Chemical & Material Sciences (AREA)
• Biomedical Technology (AREA)
• Physics & Mathematics (AREA)
• Biochemistry (AREA)
• Cell Biology (AREA)
• Food Science & Technology (AREA)
• Medicinal Chemistry (AREA)
• Biotechnology (AREA)
• Analytical Chemistry (AREA)
• Microbiology (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Pathology (AREA)
• Investigating Or Analysing Biological Materials (AREA)
• Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
• Nonwoven Fabrics (AREA)

Applications Claiming Priority (1)

Publications (1)

Family

ID=5825931

Family Applications (1)

Country Status (15)

Cited By (22)

Families Citing this family (4)

Citations (3)

• 1971
  • 1971-11-24 DE DE2158124A patent/DE2158124C3/de not_active Expired
• 1972
  • 1972-11-13 US US306127A patent/US3897214A/en not_active Expired - Lifetime
  • 1972-11-20 GB GB5352072A patent/GB1369139A/en not_active Expired
  • 1972-11-20 FI FI3263/72A patent/FI53509C/fi active
  • 1972-11-21 IT IT31925/72A patent/IT971111B/it active
  • 1972-11-21 NL NL727215725A patent/NL150231B/xx not_active IP Right Cessation
  • 1972-11-21 FR FR7241265A patent/FR2170397A5/fr not_active Expired
  • 1972-11-21 CH CH1693872A patent/CH572216A5/xx not_active IP Right Cessation
  • 1972-11-21 SE SE7215110A patent/SE401561B/xx unknown
  • 1972-11-22 ES ES408855A patent/ES408855A1/es not_active Expired
  • 1972-11-22 ZA ZA728263A patent/ZA728263B/xx unknown
  • 1972-11-22 JP JP11759372A patent/JPS5337758B2/ja not_active Expired
  • 1972-11-23 CA CA157,341A patent/CA999809A/en not_active Expired
  • 1972-11-23 AT AT999772A patent/AT318820B/de not_active IP Right Cessation
• 1978
  • 1978-02-02 HK HK68/78A patent/HK6878A/xx unknown

Patent Citations (3)

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