US3868219A - Determination of HCG glucose and galactose in body fluids with chromogenic indicators - Google Patents

Determination of HCG glucose and galactose in body fluids with chromogenic indicators Download PDF

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Publication number
US3868219A
US3868219A US365393A US36539373A US3868219A US 3868219 A US3868219 A US 3868219A US 365393 A US365393 A US 365393A US 36539373 A US36539373 A US 36539373A US 3868219 A US3868219 A US 3868219A
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United States
Prior art keywords
determination
test
paper
detection
human chorionic
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Expired - Lifetime
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US365393A
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English (en)
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Bernard Hurenkamp
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Akzona Inc
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Akzona Inc
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/81—Tube, bottle, or dipstick
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones
    • Y10S436/818—Human chorionic gonadotropin

Definitions

  • ABSTRACT [30] F i A li ti P i it D t Process for the determination of a component of a re- June 14, 1972 Netherlands 7208092 ache System which determination is based the oxidation of a chromogenic compound and in which 52 08. c1. 23/230 B, 23/253 TP, 162/78, h?" use is made test Paper emahhng 424/12 the remammg components of the reaction system. [51] Int.
  • the invention relates to a process for the determination of a component of a reaction system, which determination is based upon the oxidation of a chromogenic compound and in which determination use is made of a test paper provided with the remaining components of the reaction system, by bringing said test paper into contact with the test fluid, in which the first
  • the disturbing compound can also be removed from the system in which the determination has to be carried out by adding a suitable absorbent, such as active carbon or bentonite, or ion exchanging material.
  • glucose in urine by means of a test paper impregnated with glucoseoxidase, peroxidase and a chromogenic compound, the test results are seriously disturbed by the presence of reducing components, such as uric acid and ascorbic acid (vitamin C).
  • reducing components such as uric acid and ascorbic acid (vitamin C).
  • said treated material proves to inactivate the disturbing compounds being present in the test fluid in a very selective way.
  • the said treated material in, for example, the determination of glucose in urine, that contains uric acid and ascorbic acid as disturbing compounds, by means of glucoseoxidase, perioxidase and an oxidizable chromogenic compound, for example o-tolidine or the reduced form of 2,-dichloro-phenolindophenol, the said disturbing compounds do not have any detrimental effect on the result of the determination.
  • the said material which consists essentially of cellulose or cellulose-like material may be treated with any suitable oxidizing agent, that does not completely destruct it.
  • any suitable oxidizing agent that does not completely destruct it.
  • good results are obtained with perhalogenic acids, persalts and hypochlorites, for example, periodic acid, potassium permanganate and sodium hypochlorite.
  • a convenient and also cheap treatment-method proved to be the use of commercial bleaching liquor or the concentrated form thereof.
  • the treatment is, of course, followed by a careful removal of any excess of the oxidizing agent from the treated material obtained. Additionally the said treated material may be dried, if necessary.
  • the treated material may be used separately or in combination with a test paper any may also be the testpaper itself.
  • the treated material lends itself particularly to application on or in the test paper used for the determination of a component of a reaction system, for example, consisting of a perioxide, peroxidase and an oxidizable chromogenic compound, or consisting of a hydrogen peroxide-supplying oxido-reductase, a suitable substrate for it, a peroxidase and an oxidizable chromogenic compound.
  • the test paper contains all components of the reaction system except, of course, the component to be determined.
  • the test paper might contain one or more auxiliaries, for example a buffering substance, if required.
  • the components of the reaction system can either be used as free substances or bound to a suitable carrier, the bound form can be obtained in various ways, for example, by adsorption or by a covalent bond to the carrier.
  • the latter form is applied preferably, since it prevents the relative component from being easily rinsed from the test paper during the determination and, moreover, it offers the opportunity to determine the quantity of carrier material indirectly, if desired.
  • the test paper for determination of peroxidase may contain hydrogen peroxide that is bound to a phosphate buffer, while peroxidase itself may also be bound to a suitable carrier, for example, to human chorionic gonadotrophin (HCG).
  • HCG human chorionic gonadotrophin
  • EXAMPLE III Strips of Whatman 3 MM-paper 15 X 6 cm) were immersed, for about 16 hours, in a solution consisting of 200 ml of sodium hypochlorite g of active chlorine per 100 ml) and 200 ml of 4 N sodium hydroxide. After being washed with water until negative reaction on chlorine, the strips were dried.
  • EXAMPLE V One hundred grams of cellulose powder were treated with 500 ml of the solution from example IV for 5 hours and then washed with water until negative reaction on chlorine and neutral reaction of the washingwater. Finally the cellulose powder was air dried.
  • EXAMPLE Vl a A strip of filter paper, width 6 cm, prepared in the way as described in example IV, was washed twice in a phosphate buffer solution (consisting of 13.6 g of potassium dihydrophosphate dissolved in 800 ml of water, to which enough 4 N sodium hydroxide solution had been added to obtain a pH of 6.8, after which the volume had been completed with water to 1,000 ml) and then dried. The buffer solution was kept.
  • the prepared paper was cut into strips of 60 X 7 mm, and that crosswise, so that in each strip there was a DCPlP-zone and a pH-H-zone.
  • HCG human chorionic gonadotrophin
  • EXAMPLE Vll To 1 ml of the sample of urine mentioned in example VI (f) 50 mg of cellulose powder, pre-treated as described in example V, were added. The mixture was shaken for 1 minute, after which 0.1 ml of this fluid was tested for the presence of HCG in accordance with example Vl, but using a test paper that had not been oxidized. A marked blue colouring was formed.
  • test described in example Vl can also be performed with a test paper provided with all reagents required. This is done as follows:
  • the globulin solution is decanted and the paper finally treated with a saturated solution of B-naphtol in borate buffer of pH 8.6. This latter treatment is performed at room temperature.
  • the paper is finally washed with an 0.1 M phosphate buffer of pH 6.8 as mentioned in example V1, and dried. Then this paper is provided with the other reaction components, viz. a stroke of a reduced DCPIP solution, a stroke of urea hydrogen peroxide (starting from a 1.6% solution in ethanol) below the stroke of cellulose sheep anti-(rabbit-y-globulin) and a stroke of HCG- peroxidase conjugate and below the latter a stroke of antiserum (rabbit-anti-HCG).
  • a stroke of a reduced DCPIP solution a stroke of urea hydrogen peroxide (starting from a 1.6% solution in ethanol) below the stroke of cellulose sheep anti-(rabbit-y-globulin) and a stroke of HCG- peroxidase conjug
  • composition of reagents Composition of reagents:
  • Example Xll For the demonstration of bacteriuria advantage was taken of the circumstance that small amounts of glucose, which are always present in urine, are degradated by microorganisms. Thus, if no glucose can be demonstrated this points to the presence of a microorganism. Particularly for the test conditions prevailing here, it is essential that reducing components in the urine to be tested should be absent.
  • the paper pre-treated in a way as described in example II and provided with the required reagents in accordance with examples [X or X can be used successfully.
  • a process for the detection and determination of a member of the group consisting of human chorionic gonadotrophin, glucose, and galactose present in a fluid sample the detection and determination being based upon a visual color change produced by the oxidation of an oxidizable chromogenic compound as a component in a test reaction system including other test reaction components
  • the improvement which comprises inactivating any undesired substances which may be present in said fluid sample by first contacting the sample with a purified cellulose which has been further subjected to treatment with an oxidizing agent, and then contacting the sample with said chromogenic compound.
  • test paper for the detection and determination of human chorionic gonadotrophin in a fluid sample, the detection and determination being based upon a visual color change produced by the oxidation of an oxidizable chromogenic compound in a test reaction system including other test reaction components, said test paper consisting essentially of a purified cellulose which has been further subjected to treatment with an oxidizing agent, and being impregnated with said oxidizable chromogenic compound in a reduced form and with said remaining test reaction components including antibodies against human chorionic gonadotrophin, a conjugate of human chorionic gonadotrophin and peroxidase, insolubilized anti-antibodies, and a substrate for the peroxidase.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Emergency Medicine (AREA)
  • Endocrinology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
US365393A 1972-06-14 1973-05-30 Determination of HCG glucose and galactose in body fluids with chromogenic indicators Expired - Lifetime US3868219A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL7208092A NL7208092A (en, 2012) 1972-06-14 1972-06-14

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US (1) US3868219A (en, 2012)
JP (1) JPS4964494A (en, 2012)
AU (1) AU5664473A (en, 2012)
BE (1) BE800849A (en, 2012)
CA (1) CA1000598A (en, 2012)
CH (1) CH589293A5 (en, 2012)
DE (1) DE2330106C3 (en, 2012)
FR (1) FR2188849A5 (en, 2012)
GB (1) GB1438978A (en, 2012)
HU (1) HU168354B (en, 2012)
IE (1) IE37716B1 (en, 2012)
IT (1) IT985442B (en, 2012)
NL (1) NL7208092A (en, 2012)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4135884A (en) * 1977-08-29 1979-01-23 Shen James T Gamma stick
US4144031A (en) * 1976-04-19 1979-03-13 International Radioimmune Systems, Inc. Cell test for detecting human chorionic gonadotropin
US4168146A (en) * 1975-01-27 1979-09-18 Ab Kabi Immunoassay with test strip having antibodies bound thereto
US4189304A (en) * 1978-10-27 1980-02-19 Miles Laboratories, Inc. Device and method for detecting myoglobin
US4228127A (en) * 1978-10-06 1980-10-14 International Radioimmune Systems, Inc. Kit for detecting human chorionic gonadotropin
US4740468A (en) * 1985-02-14 1988-04-26 Syntex (U.S.A.) Inc. Concentrating immunochemical test device and method
US5654162A (en) * 1982-03-09 1997-08-05 Bio-Metric Systems, Inc. Chemical analysis apparatus and method
US6140136A (en) * 1998-09-18 2000-10-31 Syntron Bioresearch, Inc. Analytical test device and method of use
US6372514B1 (en) 1998-09-18 2002-04-16 Syntron Bioresearch, Inc. Even fluid front for liquid sample on test strip device
US20040220458A1 (en) * 2003-05-02 2004-11-04 Burd John F. Method and instruments for non-invasive analyte measurement
US20050085701A1 (en) * 2003-10-21 2005-04-21 Burd John F. Methods for non-invasive analyte measurement from the conjunctiva
US6968222B2 (en) 2003-05-02 2005-11-22 Oculir, Inc. Methods and device for non-invasive analyte measurement
US20060224057A1 (en) * 2003-10-21 2006-10-05 Oculir, Inc. Methods for non-invasive analyte measurement
US20060258919A1 (en) * 2004-04-14 2006-11-16 Oculir, Inc. Non-Invasive Analyte Measurement Device for Measuring Tears and Other Ocular Elements Using Electromagnetic Radiation and Method of Using the Same
US20080009688A1 (en) * 2004-04-14 2008-01-10 Oculir, Inc. Methods for non-invasive analyte measurement

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925789A (en) * 1986-06-30 1990-05-15 Edberg Stephen C Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3008879A (en) * 1960-03-18 1961-11-14 Miles Lab Diagnostic composition for the quantitative determination of glucose
US3092465A (en) * 1960-03-25 1963-06-04 Miles Lab Diagnostic test device for blood sugar
US3099605A (en) * 1956-11-07 1963-07-30 Miles Lab Diagnostic composition
US3123443A (en) * 1960-04-18 1964-03-03 Composition for diagnosing glucose
US3411863A (en) * 1965-07-16 1968-11-19 Agriculture Usa Process for chemically attaching compounds to aminized cellulose by means of formaldehyde
US3415361A (en) * 1966-12-22 1968-12-10 Miles Lab Test device and container therefor
US3453180A (en) * 1965-08-02 1969-07-01 Miles Lab Test article
US3598704A (en) * 1966-01-05 1971-08-10 Kabi Ab Diagnostic device for various sugars
US3619110A (en) * 1968-10-22 1971-11-09 Ugine Kuhlmann Process of hydrophilization, purification and bleaching of cellulose fibers
US3627698A (en) * 1970-03-20 1971-12-14 Boehringer Mannheim Gmbh Hydroperoxide diagnostic agents containing a chromogen indicator
US3663357A (en) * 1969-11-27 1972-05-16 Pulp Paper Res Inst Bleaching of mechanical cellulosic pulp with ozone in the presence of a peroxygen compound

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3099605A (en) * 1956-11-07 1963-07-30 Miles Lab Diagnostic composition
US3008879A (en) * 1960-03-18 1961-11-14 Miles Lab Diagnostic composition for the quantitative determination of glucose
US3092465A (en) * 1960-03-25 1963-06-04 Miles Lab Diagnostic test device for blood sugar
US3123443A (en) * 1960-04-18 1964-03-03 Composition for diagnosing glucose
US3411863A (en) * 1965-07-16 1968-11-19 Agriculture Usa Process for chemically attaching compounds to aminized cellulose by means of formaldehyde
US3453180A (en) * 1965-08-02 1969-07-01 Miles Lab Test article
US3598704A (en) * 1966-01-05 1971-08-10 Kabi Ab Diagnostic device for various sugars
US3415361A (en) * 1966-12-22 1968-12-10 Miles Lab Test device and container therefor
US3619110A (en) * 1968-10-22 1971-11-09 Ugine Kuhlmann Process of hydrophilization, purification and bleaching of cellulose fibers
US3663357A (en) * 1969-11-27 1972-05-16 Pulp Paper Res Inst Bleaching of mechanical cellulosic pulp with ozone in the presence of a peroxygen compound
US3627698A (en) * 1970-03-20 1971-12-14 Boehringer Mannheim Gmbh Hydroperoxide diagnostic agents containing a chromogen indicator

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4168146A (en) * 1975-01-27 1979-09-18 Ab Kabi Immunoassay with test strip having antibodies bound thereto
US4144031A (en) * 1976-04-19 1979-03-13 International Radioimmune Systems, Inc. Cell test for detecting human chorionic gonadotropin
US4135884A (en) * 1977-08-29 1979-01-23 Shen James T Gamma stick
US4228127A (en) * 1978-10-06 1980-10-14 International Radioimmune Systems, Inc. Kit for detecting human chorionic gonadotropin
US4189304A (en) * 1978-10-27 1980-02-19 Miles Laboratories, Inc. Device and method for detecting myoglobin
US5654162A (en) * 1982-03-09 1997-08-05 Bio-Metric Systems, Inc. Chemical analysis apparatus and method
US6020147A (en) * 1982-03-09 2000-02-01 Surmodics, Inc. Chemical analysis apparatus and method
US4740468A (en) * 1985-02-14 1988-04-26 Syntex (U.S.A.) Inc. Concentrating immunochemical test device and method
US6140136A (en) * 1998-09-18 2000-10-31 Syntron Bioresearch, Inc. Analytical test device and method of use
US6372514B1 (en) 1998-09-18 2002-04-16 Syntron Bioresearch, Inc. Even fluid front for liquid sample on test strip device
US20040220458A1 (en) * 2003-05-02 2004-11-04 Burd John F. Method and instruments for non-invasive analyte measurement
US6958039B2 (en) 2003-05-02 2005-10-25 Oculir, Inc. Method and instruments for non-invasive analyte measurement
US6968222B2 (en) 2003-05-02 2005-11-22 Oculir, Inc. Methods and device for non-invasive analyte measurement
US20050085701A1 (en) * 2003-10-21 2005-04-21 Burd John F. Methods for non-invasive analyte measurement from the conjunctiva
US6975892B2 (en) 2003-10-21 2005-12-13 Oculir, Inc. Methods for non-invasive analyte measurement from the conjunctiva
US20060224057A1 (en) * 2003-10-21 2006-10-05 Oculir, Inc. Methods for non-invasive analyte measurement
US20060258919A1 (en) * 2004-04-14 2006-11-16 Oculir, Inc. Non-Invasive Analyte Measurement Device for Measuring Tears and Other Ocular Elements Using Electromagnetic Radiation and Method of Using the Same
US20080009688A1 (en) * 2004-04-14 2008-01-10 Oculir, Inc. Methods for non-invasive analyte measurement

Also Published As

Publication number Publication date
NL7208092A (en, 2012) 1973-12-18
HU168354B (en, 2012) 1976-04-28
IE37716B1 (en) 1977-09-28
IE37716L (en) 1973-12-14
AU5664473A (en) 1974-12-12
JPS4964494A (en, 2012) 1974-06-21
DE2330106B2 (de) 1978-03-30
FR2188849A5 (en, 2012) 1974-01-18
DE2330106A1 (de) 1974-01-03
BE800849A (fr) 1973-12-13
GB1438978A (en) 1976-06-09
IT985442B (it) 1974-11-30
DE2330106C3 (de) 1978-11-30
CH589293A5 (en, 2012) 1977-06-30
CA1000598A (en) 1976-11-30

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