US3853472A - Diagnostic test strip for the detection of components of body fluids - Google Patents

Diagnostic test strip for the detection of components of body fluids Download PDF

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Publication number
US3853472A
US3853472A US00376248A US37624873A US3853472A US 3853472 A US3853472 A US 3853472A US 00376248 A US00376248 A US 00376248A US 37624873 A US37624873 A US 37624873A US 3853472 A US3853472 A US 3853472A
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US
United States
Prior art keywords
test strip
quinoline
compound
test
benzo
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Expired - Lifetime
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US00376248A
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English (en)
Inventor
W Rittersdorf
H Rey
W Guthlein
P Rieckmann
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Roche Diagnostics GmbH
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Boehringer Mannheim GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/904—Oxidation - reduction indicators

Definitions

  • Wolk Assistant Examiner-Arnold Turk Attorney, Agent, or FirmBurgess, 'Dinklage & Sprung ABSTRACT Test strips are provided for detecting even small amounts of blood or other peroxidatively active substances in body fluids; the strips comprising a carrier containing a hydroperoxide, a chromogen, and as an activator, a compound of the formula i'cdc wherein R is a hydrogen atom or a methyl radical and benzene and/or pyridine rings are fused on at least one of the positions indicated with c, (d,e), f and g, provided that two adjacent rings must not simultaneously each contain a cyclic nitrogen atom, and wherein the.
  • aromatic compounds (1) apart from the positions indicated by H and R, can be substituted by lower alkyl radicals which together can also form a hydroaromatic ring.
  • hemorrhages are caused, for example, by tumors, ulcers .and inflammations of the corresponding organsQFurthermore, free hemoglobin can also occur in the urine and plasma due to the influence of certain hemolytic toxins in the blood.
  • Bloodand hemoglobin are peroxidateactive, i.e., they liberate oxygen from hydroperoxides and transfer it to certain acceptors. Other peroxidateactive, substances occur in leukocytes and bacteria. The detection of these substances is important for the diagnosis of diseases and infections of the kidney and urinary tract.
  • Myoglobin which is also perioxidateactive, is found in the urine, for example, after a cardiac infarct. Furthermore, blood occurs especially frequently in the urine when calculi are present in the bladder or kidneys.
  • Rapid tests are usually absorbent carriers, preferably papers, which have'been impregnated with all of the reagents necessary for the detection reaction. After simply dipping them into a body fluid, they show a color reaction. Because of the great importance which rapid tests have recently achieved, they have been developed in various ways for the detection of blood in body flu.- ids.
  • German Pat. No. 1,242,905 describes test papers which, as activating additives, contain certain quinoline derivatives, preferably quinine. lt is certainly asserted that the sensitivity can be considerably increased with the mentioned additives but, acccording to our own investigations, it is practically impossible also to detect leukocytes and bacteria with the test strips improved in this manner.
  • the instant invention provides test strips for the detection of peroxidate-active substances with a hitherto unachievably high sensitivity.
  • test 5 strips comprising as the activator, an aromatic compound of the general formula:
  • R is a hydrogen atom or a methyl radical and benzene and/or pyridine rings are fused on at least one of the positions indicated with c, (d,e), f and 3, provided that two adjacent rings must not simultaneously each contain a cyclic nitrogen atom, and wherein the aromatic compounds (1), apart from the positions indi cated by H and R can be substituted by lower alkyl radicals which together can also form a hydroaromatic ring.
  • R is a hydrogen atom.
  • R is a hydrogen atom.
  • alkyl radicals there are to be understood alkyl radicals containing up to four carbon atoms and, when two alkyl radicals together form a hydroaromatic ring, 5- and 6- membered rings are preferred.
  • the compounds of general formula (I) do not increase the sensitivity of peroxidases of vegetable origin, for example horseradish peroxidase, but act specifically on peroxidate-active substances of human and animal origin. It is thus possible selectively to detect leukocytes, blood and blood components and bacteria in feces or in vomit in the presence of vegetable peroxidases. In this case, myoglobin is detected with about the same degree of sensitivity as hemoglobin.
  • the sensitivity of the detection reaction is increased by some of the compounds of general formula (l) to such an extent that even in urine it is possible to detect individual erythrocytes which are visible on the test paper as colored dots.
  • a test paper with which it is still possible clearly to detect 5 erythrocytes per mm urine. This corresponds to a blood dilution of l:l,000,000.
  • the sensitivity can be further modified by alkyl substitution; thus, for example, by means of a methyl substituent in a a-position to a cyclic nitrogen atom, the activity is somewhat reduced, whereas otherwise it usually leads to an increase of the activation.
  • the sensitivity limit of a test strip which contains a weak activator for example 2, 8- dimethyl-l,7-phenanthroline, is about erythrocytes/mm urine.
  • the activation agents according to the present invention can be used in amounts of about 0.05-1.0 percent, preferably of 0.2-0.5 percent, per 100 ml. impregnation solution.
  • an organic hydroperoxide an oxidation indicator (chromogen), a buffer and a surface-active agent, as well as, if desired, a phosphoramide, for example phosphoric acid trimorpholide, for stabilization, as well as other conventional adjuvants.
  • hydroperoxides there can be used, for example, cumol hydroperoxide or 2,5-dimethyl-hexane-2,5- dihydroperoxide, and as indicators those of the benzidine series, for example, o-tolidine, or those of the heterocyclic azines series, for example bis-(N-ethylquinol-2-one)-azine or (N-methylbenzthiazol-2-0ne)- (l-ethyl-3-phenyl-5-methyltriazol-2-one)-azine (see German Pat. No. 1,648,840).
  • benzidine series for example, o-tolidine
  • heterocyclic azines series for example bis-(N-ethylquinol-2-one)-azine or (N-methylbenzthiazol-2-0ne)- (l-ethyl-3-phenyl-5-methyltriazol-2-one)-azine (see German Pat. No. 1,648,840).
  • the indicators can be used in amounts of from 0.05-5 g., preferably of 0.2-l .0 g., per ml. of impregnation solution.
  • buffer there can be used, for example, a citrate, phosphate, phthalate or succinate buffer, the pH value and capacity being so chosen that, after dipping the test strip into a body fluid, a pH value of 4-7, preferably of 5-6, is obtained thereon.
  • test strips due to the relatively large amounts of water-soluble substances present therein, could tend to bleed, it is of practical importance to add a thickening agent to the formulation, for example methyl cellulose and, in particular, gelatine, preferably in an amount of about 0.5-5 g. per I00 ml.
  • a thickening agent for example methyl cellulose and, in particular, gelatine, preferably in an amount of about 0.5-5 g. per I00 ml.
  • wetting agent there is preferably used a longchained organic sulphate or sulphonate, for example sodium dodecyl-benzene sulphonate, dioctyl sodium sulphosuccinate or sodium lauryl sulphate, which, as is known, stabilizes radical cations, such asoxidized otolidine.
  • the wetting agents can be added to the impregnation solution in amounts of 0.5 to 5 percent, preferably of l 3 percent.
  • absorbent carriers for example filter paper, cellulose or synthetic resin fleeces
  • solutions of the reagents in readily volatile solvents This is preferably carried out in two separate steps. First, impregnation is carried out with a solution which contains a hydroperoxide, wetting agent, buffer and optionally a thickening agent. Thereafter, impregnation is carried out with a solution of an indicator and of an activator of general formula (I).
  • test strips according to the present invention are,
  • Solution 1 1.2M citrate buffer, pH 5.25 35.0 ml. ethylenediamine-tetraacetic acid, disodium salt 0.l g. dioctyl sodium sulphosuccinate 2.0 g. 2,5-dimethylhexane-2,S-dihydroperoxide (about 70%) 1.6 g. phosphoric acid trimorpholide l2.7 g. ethanol 30.0 ml. distilled water ad 100.0 ml.
  • a white test paper is obtained which, upon dipping into a blood-containing urine, becomes green colored after about 5 to seconds. If the'erythrocytes are intact, then the paper is green flecked. lf hemolysis has taken place or if free hemoglobin is present in the urine, then the paper becomes uniformly green colored.
  • the sensitivity is about 5 erythrocytes/mm or the corresponding amount of hemoglobin. A smaller number of intact erythrocytes can, under certain circumstances, still bring about individual green dots on the test paper. The sensitivity with regard to myoglobin corresponds to that for hemoglobin.
  • Leukocytes and bacteria are also detected when intact, by flecking, or when lysed, by a uniform coloration.
  • test paper obtained is about ten times less sensitive than the test papers according to Examples 1 and 2 (about 50-100 erythrocytes/mm in 3060 seconds).
  • Test papers of similar sensitivity are obtained when, instead of 3-methylbenzo [f] quinoline, there is used an equimolar amount of one of the'following activators: 1,3-dimethylbenzo [f] quinoline; 2,4-dimethylbenzo [g] quinoline; 6-methylphenanthridine; 3,8-dimethyl- 4,7-phenanthroline; 2,8-dimethyl-l,7-phenanthroline; or 2,7-dimethyll ,o-anthrazoline.
  • Test strip for the detection of peroxidatively-active substances in body fluids, comprising a carrier containing a hydroperoxide, at least one chromogen and, as an activator, a compound of the formula wherein R is hydrogen or methyl; and benzene and/or pyridine ririgs are fused on at least one of the positions indicated with c, (d,e), f and g, provided that two adjacent rings must not simultaneously contain a cyclic nitrogen atom, and wherein said compound can be substituted, other than in the positions indicated by H and R by lower alkyl radicals, which radicals together can also form a hydroaromatic ring.
  • Test strip as claimed in claim 1, wherein the carrier is an absorbent material impregnated with the reagents.
  • Test strip as claimed in claim 1, wherein the carrier is a water-stable film with the reagents incorporated therein.
  • Test strip as claimed in claim 1, wherein the reagent solution used for the production thereof contains 0.2-0.5 percent of activator per 100 ml. of solution.
  • Test strip as claimed in claim 1, wherein the reagent solution used for the production thereof contains 0.05-5 g. of chromogen per 100 ml. of solution.
  • Test strip as claimed in claim 1, wherein the reagent solution used for the production thereof contains a buffer and or a complex former, a thickener or a wetting agent.
  • Method of detecting small amounts of blood in a body fluid comprises contacting a test sample of the body fluid with a test strip as claimed in .claim 1 and observing the color formation thereon as an indication of the absence or presence of blood in said saple.
  • Method of detecting small amounts of peroxidatively active substances in a body fluid comprises contacting a test sample of the body fluid with a test strip as claimed in claim 1 and observing the color formation thereon as an indication of the absence or presence of peroxidatively active substances in said sample.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Engineering & Computer Science (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
US00376248A 1972-07-18 1973-07-03 Diagnostic test strip for the detection of components of body fluids Expired - Lifetime US3853472A (en)

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Application Number Priority Date Filing Date Title
DE2235152A DE2235152C2 (de) 1972-07-18 1972-07-18 Diagnostisches Mittel zum Nachweis von Blut und anderen peroxidatisch wirksamen Substanzen in Körperflüssigkeiten

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JP (1) JPS583680B2 (enrdf_load_stackoverflow)
AR (1) AR195135A1 (enrdf_load_stackoverflow)
AT (1) AT326276B (enrdf_load_stackoverflow)
AU (1) AU468582B2 (enrdf_load_stackoverflow)
BE (1) BE864154Q (enrdf_load_stackoverflow)
CA (1) CA988011A (enrdf_load_stackoverflow)
CH (1) CH579275A5 (enrdf_load_stackoverflow)
DD (1) DD105065A5 (enrdf_load_stackoverflow)
DE (1) DE2235152C2 (enrdf_load_stackoverflow)
FI (1) FI53511C (enrdf_load_stackoverflow)
FR (1) FR2198643A5 (enrdf_load_stackoverflow)
GB (1) GB1390900A (enrdf_load_stackoverflow)
HK (1) HK20578A (enrdf_load_stackoverflow)
HU (1) HU166368B (enrdf_load_stackoverflow)
IT (1) IT994949B (enrdf_load_stackoverflow)
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Cited By (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3966414A (en) * 1974-01-16 1976-06-29 Bio-Medical Sciences, Inc. Time temperature indicators
US3975161A (en) * 1975-02-14 1976-08-17 Lachema, Narodni Podnik Biological diagnostic test strip
US3986833A (en) * 1975-09-08 1976-10-19 Miles Laboratories, Inc. Test composition, device, and method for the detection of peroxidatively active substances
US4148611A (en) * 1978-06-28 1979-04-10 Miles Laboratories, Inc. Test composition, device and method for the detection of peroxidatively active substances
US4175923A (en) * 1978-06-26 1979-11-27 Friend William G Method and apparatus for occult blood testing in the home
EP0014929A1 (de) * 1979-02-14 1980-09-03 Roche Diagnostics GmbH Diagnostisches Mittel, Verfahren zu dessen Herstellung sowie dessen Verwendung zum Nachweis von Leukozyten in Körperflüssigkeiten
US4251222A (en) * 1979-12-17 1981-02-17 Miles Laboratories, Inc. Sensitizers for peroxidative activity tests
US4251223A (en) * 1979-12-17 1981-02-17 Miles Laboratories, Inc. Sensitizers for peroxidative activity tests
US4278439A (en) * 1979-12-17 1981-07-14 Miles Laboratories, Inc. Sensitizers for peroxidative activity tests
US4339243A (en) * 1979-11-13 1982-07-13 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4339242A (en) * 1979-11-13 1982-07-13 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340395A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340393A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340394A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340392A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4380585A (en) * 1979-11-13 1983-04-19 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4388271A (en) * 1980-01-10 1983-06-14 Rohm Gmbh Rapid diagnostic agents
US4673654A (en) * 1985-10-31 1987-06-16 Warner-Lambert Company Composition for determining peroxidase-like activity of hemoglobin
US4676950A (en) * 1984-02-03 1987-06-30 Foster Research Corporation Indicator and test device for detecting occult blood
US4755472A (en) * 1986-01-16 1988-07-05 Miles Inc. Stable composition for the determination of peroxidatively active substances
US4849342A (en) * 1985-01-31 1989-07-18 Savyon Diagnostics Limited Method for carrying out enzyme assays
US4956300A (en) * 1982-01-05 1990-09-11 Helena Laboratories Corporation Aid for determining the presence of occult blood, method of making the aid, and method of using the aid
US5081040A (en) * 1987-06-29 1992-01-14 Helena Laboratories Corporation Composition and kit for testing for occult blood in human and animal excretions, fluids, or tissue matrixes
US5196167A (en) * 1989-04-04 1993-03-23 Helena Laboratories Corporation Fecal occult blood test product with positive and negative controls
US5217874A (en) * 1989-04-04 1993-06-08 Helena Laboratories Corporation Fecal occult blood test product with positive and negative controls
US5273888A (en) * 1984-01-16 1993-12-28 Helena Laboratories Corporation Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals
US5369013A (en) * 1987-06-22 1994-11-29 Yissum Research Development Company Of The Hebrew University Of Jerusalem Method, reagent mixture and kit for determining the presence of bacterial or somatic cells in urine
US5426032A (en) * 1986-08-13 1995-06-20 Lifescan, Inc. No-wipe whole blood glucose test strip
WO1997035030A1 (en) * 1996-03-22 1997-09-25 Stc Technologies, Inc. A solution-based assay for peroxidatively-active substances in bodily fluids
US5702913A (en) * 1983-12-21 1997-12-30 Helena Laboratories Corporation Chromgen-reagent test system
US5827677A (en) * 1996-02-01 1998-10-27 Universal Lavel Method and device for specifically detecting myoglobin using a non-discriminating peroxidase sensitive assay
US5885789A (en) * 1997-03-20 1999-03-23 Stc Technologies Incorporated Solution-based assay for peroxidatively-active substances in bodily fluids
US6458326B1 (en) 1999-11-24 2002-10-01 Home Diagnostics, Inc. Protective test strip platform
US6525330B2 (en) 2001-02-28 2003-02-25 Home Diagnostics, Inc. Method of strip insertion detection
US6541266B2 (en) 2001-02-28 2003-04-01 Home Diagnostics, Inc. Method for determining concentration of an analyte in a test strip
US6562625B2 (en) 2001-02-28 2003-05-13 Home Diagnostics, Inc. Distinguishing test types through spectral analysis
US7846383B2 (en) 2006-12-15 2010-12-07 Kimberly-Clark Worldwide, Inc. Lateral flow assay device and absorbent article containing same
US8012761B2 (en) 2006-12-14 2011-09-06 Kimberly-Clark Worldwide, Inc. Detection of formaldehyde in urine samples

Families Citing this family (7)

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DE2436598C2 (de) * 1974-07-30 1983-04-07 Boehringer Mannheim Gmbh, 6800 Mannheim Stabiler Teststreifen zum Nachweis von Inhaltsstoffen in Flüssigkeiten
DE2716060C3 (de) * 1977-04-09 1980-06-12 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisierte Schnelldiagnostica mit Oxydationsindikatoren
DE2926271A1 (de) * 1979-06-29 1981-01-08 Behringwerke Ag Mittel zum nachweis peroxidatisch wirksamer substanzen
CA1143634A (en) * 1980-06-02 1983-03-29 Alan E. Burkhardt Interference-resistant test device for determining a peroxidately active substance in a test sample and method for preparing it
US4672029A (en) * 1984-12-06 1987-06-09 Eastman Kodak Company Color-forming couplers and their use in the analytical determination of hydrogen peroxide or other analytes
EP0196743A3 (en) * 1985-01-31 1988-10-19 Savyon Diagnostics Ltd. Stable chemical compositions containing chromogenic materials and peroxides, and method for obtaining them
DE19500768C2 (de) * 1994-01-17 2003-11-20 Aventis Res & Tech Gmbh & Co Phenanthren-Derivate und ihre Verwendung in flüssigkristallinen Mischungen

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US3290117A (en) * 1963-06-24 1966-12-06 Miles Lab Occult blood diagnostic composition with color enhancing agent
US3712853A (en) * 1969-08-14 1973-01-23 Boehringer Mannheim Gmbh Diagnostic agent for the detection of nitrite and of nitrite-forming bacteria

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US3290117A (en) * 1963-06-24 1966-12-06 Miles Lab Occult blood diagnostic composition with color enhancing agent
US3712853A (en) * 1969-08-14 1973-01-23 Boehringer Mannheim Gmbh Diagnostic agent for the detection of nitrite and of nitrite-forming bacteria

Cited By (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3966414A (en) * 1974-01-16 1976-06-29 Bio-Medical Sciences, Inc. Time temperature indicators
US3975161A (en) * 1975-02-14 1976-08-17 Lachema, Narodni Podnik Biological diagnostic test strip
US3986833A (en) * 1975-09-08 1976-10-19 Miles Laboratories, Inc. Test composition, device, and method for the detection of peroxidatively active substances
JPS5233594A (en) * 1975-09-08 1977-03-14 Miles Lab Testing constitutes* instruments and methods for detection of peroxidation active substances
US4175923A (en) * 1978-06-26 1979-11-27 Friend William G Method and apparatus for occult blood testing in the home
US4148611A (en) * 1978-06-28 1979-04-10 Miles Laboratories, Inc. Test composition, device and method for the detection of peroxidatively active substances
US4299917A (en) * 1979-02-14 1981-11-10 Boehringer Manneheim Gmbh Diagnostic agents for the detection of leukocytes in body fluids
EP0014929A1 (de) * 1979-02-14 1980-09-03 Roche Diagnostics GmbH Diagnostisches Mittel, Verfahren zu dessen Herstellung sowie dessen Verwendung zum Nachweis von Leukozyten in Körperflüssigkeiten
US4339243A (en) * 1979-11-13 1982-07-13 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4380585A (en) * 1979-11-13 1983-04-19 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340392A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340394A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340393A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4339242A (en) * 1979-11-13 1982-07-13 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340395A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4251223A (en) * 1979-12-17 1981-02-17 Miles Laboratories, Inc. Sensitizers for peroxidative activity tests
US4278439A (en) * 1979-12-17 1981-07-14 Miles Laboratories, Inc. Sensitizers for peroxidative activity tests
EP0030682A1 (en) * 1979-12-17 1981-06-24 Miles Laboratories, Inc. Test composition for peroxidatively active substances, test device containing the composition, process for preparing same and method for the determination of peroxidatively active substances
US4251222A (en) * 1979-12-17 1981-02-17 Miles Laboratories, Inc. Sensitizers for peroxidative activity tests
US4388271A (en) * 1980-01-10 1983-06-14 Rohm Gmbh Rapid diagnostic agents
US4956300A (en) * 1982-01-05 1990-09-11 Helena Laboratories Corporation Aid for determining the presence of occult blood, method of making the aid, and method of using the aid
US5702913A (en) * 1983-12-21 1997-12-30 Helena Laboratories Corporation Chromgen-reagent test system
US5273888A (en) * 1984-01-16 1993-12-28 Helena Laboratories Corporation Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals
US4676950A (en) * 1984-02-03 1987-06-30 Foster Research Corporation Indicator and test device for detecting occult blood
US4849342A (en) * 1985-01-31 1989-07-18 Savyon Diagnostics Limited Method for carrying out enzyme assays
US4673654A (en) * 1985-10-31 1987-06-16 Warner-Lambert Company Composition for determining peroxidase-like activity of hemoglobin
US4755472A (en) * 1986-01-16 1988-07-05 Miles Inc. Stable composition for the determination of peroxidatively active substances
US6268162B1 (en) 1986-08-13 2001-07-31 Lifescan, Inc. Reflectance measurement of analyte concentration with automatic initiation of timing
US6858401B2 (en) 1986-08-13 2005-02-22 Lifescan, Inc. Minimum procedure system for the determination of analytes
US6887426B2 (en) 1986-08-13 2005-05-03 Roger Phillips Reagents test strip adapted for receiving an unmeasured sample while in use in an apparatus
US5426032A (en) * 1986-08-13 1995-06-20 Lifescan, Inc. No-wipe whole blood glucose test strip
US5563042A (en) * 1986-08-13 1996-10-08 Lifescan, Inc. Whole blood glucose test strip
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FR2198643A5 (enrdf_load_stackoverflow) 1974-03-29
AR195135A1 (es) 1973-09-10
DE2235152A1 (enrdf_load_stackoverflow) 1974-01-10
SE388693B (sv) 1976-10-11
DE2235152B1 (de) 1974-01-10
ATA628773A (de) 1975-02-15
HU166368B (enrdf_load_stackoverflow) 1975-03-28
AU5826373A (en) 1975-01-23
BE864154Q (fr) 1978-08-21
NL7309779A (enrdf_load_stackoverflow) 1974-01-22
JPS4954094A (enrdf_load_stackoverflow) 1974-05-25
JPS583680B2 (ja) 1983-01-22
CH579275A5 (enrdf_load_stackoverflow) 1976-08-31
NL152085B (nl) 1977-01-17
AT326276B (de) 1975-12-10
CA988011A (en) 1976-04-27
DE2235152C2 (de) 1975-07-10
FI53511C (fi) 1978-05-10
ZA734810B (en) 1974-07-31
HK20578A (en) 1978-04-20
DD105065A5 (enrdf_load_stackoverflow) 1974-04-05
IT994949B (it) 1975-10-20
AU468582B2 (en) 1976-01-15
FI53511B (enrdf_load_stackoverflow) 1978-01-31
GB1390900A (en) 1975-04-16

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