US3645853A - Diagnostic composition and method for the detection of nitrate reduction - Google Patents

Diagnostic composition and method for the detection of nitrate reduction Download PDF

Info

Publication number
US3645853A
US3645853A US836089A US3645853DA US3645853A US 3645853 A US3645853 A US 3645853A US 836089 A US836089 A US 836089A US 3645853D A US3645853D A US 3645853DA US 3645853 A US3645853 A US 3645853A
Authority
US
United States
Prior art keywords
zone
nitrate
reagent
diagnostic composition
zones
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US836089A
Other languages
English (en)
Inventor
Donald P Kronish
William D Young Jr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Warner Lambert Co LLC
Original Assignee
Warner Lambert Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Warner Lambert Co LLC filed Critical Warner Lambert Co LLC
Application granted granted Critical
Publication of US3645853A publication Critical patent/US3645853A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/12—Nitrate to nitrite reducing bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/805—Test papers

Definitions

  • ABSTRACT A diagnostic composition and method for detecting the reduction of nitrate to nitrite by micro-organisms which comprises a carrier material impregnated in specific zones with a nitratecontaining medium, a barrier composition, and two stable re- 1 1 Claims, 1 Drawing Figure RAIENIEDfEazswsn AREA l l4mm ITAREAIZT AREA 5 l4 mm AREA 4 l6 mm AREAS l6.5 mm
  • the taxonomic value of the nitrate reduction test for the identification and differentiation of micro-organisms which are pathogenic to humans is well known.
  • the Enteriobacteriaceae family react positively to the nitrate reduction test. Certain bacteria reduce nitrate to nitrite only while others continue the reduction and transform nitrite compounds into free nitrogen or ammonia.
  • nutrient medium containing potassium nitrate (nitrite free) is inoculated with a pure culture of the strain under examination and incubated at 37 C. for 24 hours.
  • the medium is tested for the presence of nitrites by adding 0.1 ml. of a mixture of test reagents prepared from 0.08% w/v sulfanilic acid in 5 N acetic acid; and 0.5% w/v a-naphthylamine I-aminonaphthalene) in 5 N acetic acid. These reagents are mixed together immediately before use.
  • Another object of this invention is to provide a diagnostic composition in which all the required nutrients and reagents for said differentiation test are provided in a premeasured, stable, easy-to-use form.
  • a rapid, stable diagnostic composition and method for the identification and differentiation of micro-organisms which reduce nitrate to nitrite is provided in the form of an impregnated carrier material.
  • the use of the composition of this invention makes it possible to enable one to differentiate between micro-organisms, which do or do not reduce nitrate in as little as about 2 hours since it is sensitive to the detection of as little as l microgram of nitrite.
  • the diagnostic composition provided is stable for at least 12 months at room temperature.
  • the diagnostic composition of this invention is prepared by impregnating certain specified areas of a bibulous carrier material with a solution of a nitrate-containing medium (hereinafter designated Zone A Nitrate Medium) and stable reagent solutions (hereinafter designated Zone C and Zone D Reagents), wherein the nitrate medium is separated from the reagent zone area by a hydrophobic barrier composition (hereinafter designated Zone B Hydrophobic Barrier).
  • Zone A Nitrate Medium a solution of a nitrate-containing medium
  • Zone C and Zone D Reagents stable reagent solutions
  • An additional barrier zone, an untreated area and a dyed identification zone are optionally present in the preferred diagnostic composition.
  • the impregnated bibulous carrier material is cut into individual strips containing sufficient quantities of all the ingredients necessary for the identification and differentiation of micro-organisms by their ability to reduce nitrate to nitrite.
  • the Zone A nitrate-containing medium can be any composition containing a nontoxic nitrate salt alone, or, preferably in combination with a nutrient medium such as peptone.
  • a preferred medium for example is prepared by forming an aqueous solution of beef extract, peptone and potassium nitrate.
  • a particularly preferred medium of this type marketed as BACTO-NITRATE BROTH (dehydrated) by Difco Laboratories, Detroit, Michigan, contains these components in a ratio of 3 g. of beef extract, to 5 g. of peptone and l g. of potassium nitrate. It has been found that a [00 ml. distilled water solution of from about 10 to 27 g. of dehydrated BACTO-NITRATE BROTH is quite suitable for use in the preparation of the diagnostic composition of this invention.
  • the composition used is one which will prevent the premature leaching of the culture during incubation upward into the reagent zone.
  • the barrier composition must, of course, be chemically and biologically inert in this test system. Any substance which will form a waterproof barrier of this type may be sued. Suitable materials include waxes, lacquers, and plastics, particularly that colorless polymerized methyl methacrylate coating composition marketed by Krylon, Inc., Norristown, Pa. under the trade name KRYLON 150 CRYSTAL CLEAR. The KRYLON material is particularly preferred. It is supplied in a toluene vehicle and may be diluted for ease of application with additional toluene or other diluents, such as ethyl, methyl, or propyl alcohol USP.
  • barrier solution prepared from about 75 to ml. of KRYLON and to which is added 0 to 25 ml. of diluent is suitable.
  • Zone C Reagent contains an aminosubstituted-naphthalene sulfonic acid and an alkali metal salt of sulfanilic acid or, alternately, sulfadiazine or an alkali metal salt of sulfathiazole while Zone D Reagent contains a crystalline acid such as oxalic, malonic or citric acid.
  • the amino-substituted-naphthalene sulfonic acid may, for example, be 5- amino-Z-naphthalene sulfonic acid, 8-amino-2naphthalene sulfonic acid or S-amino-l-naphthalene sulfonic acid, with the S-amino-l-naphthalene sulfonic acid preferred among the three acids.
  • the salt of sulfanilic acid the sodium salt is preferred.
  • the two reagent impregnated zones are positioned adjacent to each other on the individual diagnostic test product, either in a side-by-side relationship on the same side of the carrier material or, preferably, positioned back-to-back with one of the reagent solutions impregnated on one side and the other reagent on the other side of the same area of the carrier material.
  • the two reagent solutions are applied to the bibulous carrier material separately, allowing drying between applications to prevent premature mixing of the reagents prior to wetting during the performance of the test.
  • Zone C Reagent solution is prepared from a 100 ml. distilled water solution of:
  • Zone C Reagent The pH of the final solution of Zone C Reagent is adjusted to from about 7 to 12, preferably from about 9.8 to 10, with a suitable pH modifying agent which will not interfere with the diagnostic test, suchas a sodium, potassium,-or ammonium hydroxide; preferably an 0.1 N sodium hydroxide solution is used.
  • a suitable pH modifying agent which will not interfere with the diagnostic test, suchas a sodium, potassium,-or ammonium hydroxide; preferably an 0.1 N sodium hydroxide solution is used.
  • an interim pH adjustment is conveniently made on an aqueous solution of the amino-substituted-naphthalene sulfonic acid,
  • a pH modifying agent preferably 1 N sodium hydroxide
  • Zone D Reagent solution is prepared from a solution of from about 20 to 65 g., and preferably about 35 to 60 g. in 100 ml. of distilled water of at least one of the crystalline acids such as oxalic, malonic or citric acid.
  • the Zone D Reagent solution described is preferably applied to the reverse side of that portion of the reagent zone on the carrier material which carries the Zone C Reagent solution.
  • one end of the impregnated test strip may contain additional, zones to prevent the contamination of the nitrate medium and reagent zones through handling.
  • additional barrier zone hereinafter designated Zone B hydrophobic barrier
  • a dyed identification zone hereinafter designated Zone E Dye may be present contiguous to barrier Zone B Barrier, or separated from this second barrier zone by an untreated area.
  • Zone l3 hydrophobic barrier composition is also suitable for use as the Zone B hydrophobic barrier.
  • Any suitable dye which will color the bibulous material sufficiently to distinguish the end of the diagnostic test strip which is to be handled from the colorless reagent zones which are to be inserted into the culture under investigation may be used.
  • About 0.025 to 0.3 g. of a dye, dissolved in a suitable solvent and adjusted to a volume of 100 ml. has been found to provide a suitable identification Zone E solution.
  • the preferred dye solution is prepared from brilliant green, a biological stain (Matheson, Coleman and Bell) which is soluble in water.
  • Methyl Green National Aniline.
  • the bibulous materials suitable as the carrier for the diagnostic composition of this invention are those materials which, by means of capillary action, are able to hold liquid. Such materials include filter paper, felt, porous ceramic strips, woven or matted glass fiber and the like. A particularly preferred pater is Eaton-Dikeman No. 623 (70 lbs).
  • a single-diagnostic composition contains:
  • Zone A about 2.8 mg. of nitrate broth prepared from beef extract, peptone and potassium nitrate; Area 2:
  • Zone 8 saturated with a solution of about ml. of a methyl methacrylate resin coating composition and about 15 ml. of ethyl alcohol;
  • Zone C about 0.05 mg. of 5amino-l-naphthalene sulfonic acid and about 0.08 mg. of the sodium salt of sulfanilic acid; Area 3:-
  • Zone D about 3 mg. of citric acid
  • Zone 8 totally saturated with the Zone B solution; Area 5: I
  • Zone E visibly colored by the application of a ml. aqueous solution of 0.1 g. of brilliant green.
  • the diagnostic composition of this invention is stable for at least 12 months at 4 C. and at room temperature.
  • a loopful of the culture to be tested is suspended in 0.3 ml. of saline in a l3Xl00 mm. or similar size test tube.
  • the diagnostic composition test strip of this invention is inserted in the tube in such a manner that the Zone A nitrate medium is immersed in the test suspension.
  • the tube is incubated at from'35 to 37 for about 1 A to 2 hours.
  • the tube is then tipped to wet the reagent zone areas and the development of a pink to red color in the reagent zone in 30 seconds to 10 minutes indicates a positive result, i.e., that nitrate has been reduced to nitrite. No color change or a light buff color indicates a negative test.
  • the diagnostic composition of this invention will detect the presence of 1 microgram of nitrite ion present in 0.3 ml. suspension ofa culture to be tested.
  • the diagnostic composition and procedure of this invention was compared with the results obtained using classical method of Edwards and Ewing (Identification of Enterobacteriaceae, P. R. Edwards and W. H. Ewing, Burgess Pub. Co. 1962, printed 1964). A total of 107 organisms were treated. Results are listed in Table 1 below. 106 of the cultures gave the same reaction with both procedures, a correlation of better than 99 1 Strip-broth weak.
  • EXAMPLE 1 A. Preparation of Zone A Nitrate Medium Solution To 60 ml. of distilled water, add g. of Bacto-Nitrate Broth, dehydrated (Difco). Mix well, and adjust volume to 100 ml. with distilled water. Heat to 100 C., and boil briefly to obtain a clear solution, then cool to C. Use for application to test strip within 2 hours of preparation.
  • Zone B Preparation of Zone B, and Zone B Hydrophobic Barrier Composition Dilute 85 ml. of Krylon 150 Crystal Clear with 15 ml. of 95 percent ethanol, USP.
  • Zone C Preparation of the Zone C reagent Solution, Amino Substituted Naphthalene Sulfonic Acid and Sulfanilic Acid Salt
  • To 50 ml. of distilled water add 1 g. of S-amino-lnaphthalene sulfonic acid. Slowly add, with mixing, 1 N sodium hydroxide solution until a stable pH of 9.5-10 is obtained.
  • Add 1.6 g. of sulfanilic acid, sodium salt mix to dissolve and adjust to a pH of 9.8l0 with 0.1 N NaOH solution. Adjust volume to 100 ml. with distilled water and mix.
  • Zone D Reagent Solution Citric Acid
  • 50 g. citric acid add sufficient distilled water to obtain a final volume of 100 ml. Mix and warm as necessary to dissolve.
  • Zone E Preparation of Zone E, Dye Solution Dissolve 0.1 g. brilliant green, biological stain (Matheson, Coleman & Bell) in distilled water, adjust volume to 100 ml. with distilled water and mix.
  • Zone B hydrophobic Barrier Solution of Example 1 to areas 2 and 4 in amounts sufficient to saturate the paper and allow to dry.
  • Zone D Reagent Solution of Example 1 to the reverse side of area 3 so that no more than one-half the thickness of the paper is wet.
  • the reagent solutions must be applied carefully in order that Zone C reagent solution does not mix with Zone D reagent solution.
  • the nitrate broth is applied to the paper at a rate of 0.70 ml. for each cm. of length with the sulfanilic acid and the S-amino-naphthalene sulfonic acid being applied at a rate of 0.25 ml. and the citric acid solution being applied at a rate of0.3 ml. per 30 cm.
  • EXAMPLE 3 Use of the Diagnostic Composition Method of Use l. Suspend a loopful of culture from an agar medium in 0.3
  • Zone B Preparation of Zone B, and Zone B Hydrophobic Barrier Solution.
  • Methyl Green National Aniline
  • EXAMPLE 5 Apply the Zone A, B, C, D and E solutions, prepared according to Example 4 to the bibulous material carrier as described in Example 2.
  • EXAMPLE 6 follows the procedure of Example 3, using the diagnostic composition test strip prepared according to Examples 4 and 5.
  • a diagnostic composition for the detection of nitrite which comprises a bibulous material containing at least four impregnated zones, wherein:
  • Zone A contains a nitrate-containing medium
  • Zone B contains a dried inert hydrophobic barrier composition, separating Zone A from all succeeding impregnated zones
  • Zone C contains (1) an alkali metal salt of an amino-substitutednaphthalene sulfonic acid of the group consisting of 5- amino-2-naphthalene-sulfonic acid, 8-amino-2- naphthalene-sulfonic acid, and S-aminol naphthalene-sulfonic acid, and
  • Zone D contains at least one crystalline acid of the group consisting of citric acid, oxalic acid and malonic acid; said zones being arranged in an order which will promote the development of color in the reagent Zones C and D to detect any reduction of the nitrate present to nitrite by micro-organisms incubated in the presence of said nitrate-containing medium Zone A.
  • a diagnostic composition for the detection of nitrite which comprises a bibulous material containing at least four impregnated zones, wherein:
  • Zone A contains from about 1.4 to about 4.6 mg. of a nitrate-containing nutrient medium
  • Zone B contains a dried inert hydrophobic barrier composition, separating Zone A from all succeeding impregnated zones;
  • Zone C contains 1 from about 0.005 to about 0.2 mg. of an alkali metal salt of an amino-substituted-naphthalene sulfonic acid of the group consisting of 5-amino-2-naphthalene-sulfonic acid, 8-amino-2-naphthalene-sulfonic acid, and S-amino- 1 -naphthalene-sulfonic acid, and
  • Zone D contains from about 1 to about 4.5 mg. of at least one crystalline acid of the group consisting of citric acid, oxalic acid and malonic acid; said zones being arranged in an order which will promote the development of color in the reagent Zones C and D to detect any reduction of the nitrate present to nitrite by micro-organisms incubated in the presence of said nitrate-containing nutrient medium Zone A.
  • a diagnostic composition for the detection of nitrite which comprises a bibulous material containing at least four reagentimpregnated zones, wherein:
  • Zone A contains from about 2 to about 4 mg. ofa nitratecontaining nutrient medium
  • Zone B contains a sufficient amount of a dried inert hydrophobic barrier composition to saturate Zone B, and separate Zone A from all succeeding impregnated zones, said inert hydrophobic barrier composition comprising an acrylic coating composition;
  • Zone C contains 1. from about 0.01 to about 0.1 mg. of an alkali metal salt of an amino-substituted-naphthalene sulfonic acid of the group consisting of 5-amino-2-naphthalene-sulfonic acid, 8-amino-2-naphthalene-sulfonic acid, and S-amino-l-naphthalene-sulfonic acid, and
  • Zone D contains from about 2 to about 4 mg. of at least one crystalline acid of the group consisting of citric acid, oxalic acid, and malonic acid; said zones being arranged in an order which will promote the development of color in the reagent Zones C and D to detect any reduction of the nitrate present to nitrite by micro-organisms incubated in the presence of said nitrate-containing nutrient medium Zone A.
  • a diagnostic composition for the detection of nitrite which comprises a bibulous material containing at least four reagent impregnated zones, wherein:
  • Zone A contains about 2.8 mg. of a nutrient medium prepared from about 3 mg. of beef extract, about 5 mg. of peptone and about 1 mg. of potassium nitrate;
  • Zone B contains a sufficient amount of a dried inert hydrophobic barrier composition to saturate Zone B, said hydrophobic barrier composition comprising a methyl methacrylate coating composition;
  • Zone C contains 1. about 0.05 mg. of the sodium salt of 5-amino-lnaphthalene sulfonic acid, and 2. about 0.08 mg. ,of the sodium salt of sulfanilic acid;
  • Zone D contains about 3 mg. of citric acid; wherein the zones are positioned on the bibulous material in the sequence A, B, C with Zone D applied to the bibulous material on the reverse side of Zone 0 and wherein Zone A 15 contiguous only to Zone B; Zone B 15 contiguous only to Zone A and Zone C on one side of the bibulous material and contiguous only to Zone A and Zone D on the reverse side of the bibulous material; and Zone C and Zone D are positioned in a back-to-back relationship to each other and are each contiguous only to Zone B and to each other.
  • a process for the identification and differentiation of micro-organisms which reduce nitrate to nitrite which comprises:

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
US836089A 1969-06-24 1969-06-24 Diagnostic composition and method for the detection of nitrate reduction Expired - Lifetime US3645853A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US83608969A 1969-06-24 1969-06-24

Publications (1)

Publication Number Publication Date
US3645853A true US3645853A (en) 1972-02-29

Family

ID=25271215

Family Applications (1)

Application Number Title Priority Date Filing Date
US836089A Expired - Lifetime US3645853A (en) 1969-06-24 1969-06-24 Diagnostic composition and method for the detection of nitrate reduction

Country Status (7)

Country Link
US (1) US3645853A (enExample)
JP (1) JPS5413508B1 (enExample)
CH (1) CH541626A (enExample)
DE (1) DE2030720C3 (enExample)
FR (1) FR2051227A5 (enExample)
GB (1) GB1246080A (enExample)
SE (1) SE380044B (enExample)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2161979A2 (enExample) * 1971-11-30 1973-07-13 Warner Lambert Co
US3957584A (en) * 1974-09-30 1976-05-18 Warner-Lambert Company Detection of beta-galactosidase producing micro-organisms
US4129417A (en) * 1977-10-28 1978-12-12 Miles Laboratories, Inc. Multisystem test means
US4434235A (en) 1981-01-21 1984-02-28 Ben Gurion University Of The Negev Research And Development Authority Method and apparatus for detecting nitrite ions in fluids
US4631255A (en) * 1984-07-23 1986-12-23 Eiken Kagaku Kabushiki Kaisha Composition for assaying for nitrites
CN1100988C (zh) * 1998-08-17 2003-02-05 中国科学院动物研究所 监测害虫对有机磷杀虫剂抗性的试纸及其制备方法
US20040161365A1 (en) * 2001-12-05 2004-08-19 Yeung Siu Yu Test strips having a plurality of reaction zones and methods for using and manufacturing the same
CN100427928C (zh) * 2005-01-17 2008-10-22 浙江大学 硝酸盐、亚硝酸盐快速测定试纸及其应用
WO2012010708A1 (de) 2010-07-23 2012-01-26 Aj Innuscreen Gmbh Verfahren, vorrichtung und testkit für molekularbiologische reaktionen

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3341427A (en) * 1965-01-22 1967-09-12 Warner Lambert Pharmaceutical Diagnostic preparation and process for the detection of acetylmethylcarbinol
US3378346A (en) * 1965-02-05 1968-04-16 Warner Lambert Pharmaceutical Diagnostic preparation for the detection of indole
US3547780A (en) * 1968-03-13 1970-12-15 Frank A Finnerty Jr Simplified accurate method of detecting bacteriuria

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3341427A (en) * 1965-01-22 1967-09-12 Warner Lambert Pharmaceutical Diagnostic preparation and process for the detection of acetylmethylcarbinol
US3378346A (en) * 1965-02-05 1968-04-16 Warner Lambert Pharmaceutical Diagnostic preparation for the detection of indole
US3547780A (en) * 1968-03-13 1970-12-15 Frank A Finnerty Jr Simplified accurate method of detecting bacteriuria

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Welcher, Organic Analyt. Reagents Vol. IV pp. 574 583 (1948). *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2161979A2 (enExample) * 1971-11-30 1973-07-13 Warner Lambert Co
US3957584A (en) * 1974-09-30 1976-05-18 Warner-Lambert Company Detection of beta-galactosidase producing micro-organisms
US4129417A (en) * 1977-10-28 1978-12-12 Miles Laboratories, Inc. Multisystem test means
US4434235A (en) 1981-01-21 1984-02-28 Ben Gurion University Of The Negev Research And Development Authority Method and apparatus for detecting nitrite ions in fluids
US4631255A (en) * 1984-07-23 1986-12-23 Eiken Kagaku Kabushiki Kaisha Composition for assaying for nitrites
CN1100988C (zh) * 1998-08-17 2003-02-05 中国科学院动物研究所 监测害虫对有机磷杀虫剂抗性的试纸及其制备方法
US20040161365A1 (en) * 2001-12-05 2004-08-19 Yeung Siu Yu Test strips having a plurality of reaction zones and methods for using and manufacturing the same
CN100427928C (zh) * 2005-01-17 2008-10-22 浙江大学 硝酸盐、亚硝酸盐快速测定试纸及其应用
WO2012010708A1 (de) 2010-07-23 2012-01-26 Aj Innuscreen Gmbh Verfahren, vorrichtung und testkit für molekularbiologische reaktionen
DE102010038330A1 (de) * 2010-07-23 2012-03-01 Aj Innuscreen Gmbh Verfahren, Vorrichtung und Testkit für Molekularbiologische Reaktionen

Also Published As

Publication number Publication date
SE380044B (enExample) 1975-10-27
DE2030720A1 (de) 1971-01-07
CH541626A (de) 1973-09-15
FR2051227A5 (enExample) 1971-04-02
DE2030720B2 (de) 1978-01-26
JPS5413508B1 (enExample) 1979-05-31
GB1246080A (en) 1971-09-15
DE2030720C3 (de) 1978-09-21

Similar Documents

Publication Publication Date Title
US3814670A (en) Test article for use in microbiology
CA1288322C (en) Detection of microbes in a sample
US4207394A (en) Process and apparatus for analyzing specimens for the presence of microorganisms therein
US3712853A (en) Diagnostic agent for the detection of nitrite and of nitrite-forming bacteria
US3395082A (en) Test composition device and method for detecting urea in aqueous fluids
DE69738256T2 (de) Nachstummedium für den auswählenden nachweis von staphylokokkus
US3645853A (en) Diagnostic composition and method for the detection of nitrate reduction
US3785929A (en) Diagnostic composition for the detection of nitrite
US4390622A (en) Neisseria bacteria species identification and beta lactamase testing methods
US3427225A (en) Test composition,device and method for detecting urea in aqueous fluids
CH615460A5 (enExample)
NZ268405A (en) Atp-adp chemiluminescent testing for microorganisms including a source of a magnesium ion
US4225783A (en) Determination of microbial cells in aqueous samples
Bennett et al. Identification of fusobacteria in a routine diagnostic laboratory
US4532206A (en) β-Streptococcus selective medium
US3359180A (en) Diagnostic preparation for the detection of acetylmethylcarbinol
WO1986000340A1 (en) Processes and materials for carrying out microchemical and microbiological tests
US3616258A (en) Diagnostic product and process for the detection of niacin production by mycobacteria
US3415717A (en) Bacteriuria test papers
US3597321A (en) Diagnostic compostion for the differentiation of staphylococci
CA1060764A (en) E. coli detection broth for clinical use with automated microbial analyzer
GB2138443A (en) Device for identifying microorganisms
CA1215616A (en) Diagnostic test probe
US3216907A (en) Fermentation process for determining microbial sensitivity to antimicrobial agents
US3649461A (en) Paper strip test for citrate utilization