US3616265A - Device for making a culture of microorganisms - Google Patents

Device for making a culture of microorganisms Download PDF

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Publication number
US3616265A
US3616265A US848212A US3616265DA US3616265A US 3616265 A US3616265 A US 3616265A US 848212 A US848212 A US 848212A US 3616265D A US3616265D A US 3616265DA US 3616265 A US3616265 A US 3616265A
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US
United States
Prior art keywords
container
elongated member
culture medium
brush
guide member
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US848212A
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English (en)
Inventor
Anthony J Calabrese
Joseph F Pagano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Smith Kline and French Laboratories Ltd
SmithKline Beecham Corp
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Smith Kline and French Laboratories Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smith Kline and French Laboratories Ltd filed Critical Smith Kline and French Laboratories Ltd
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Publication of US3616265A publication Critical patent/US3616265A/en
Assigned to SMITHKLINE BECKMAN CORPORATION reassignment SMITHKLINE BECKMAN CORPORATION CHANGE OF NAME (SEE DOCUMENT FOR DETAILS). EFFECTIVE DATE: 03/04/82 Assignors: SMITHKLINE CORPORATION
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts

Definitions

  • a device for making a culture of micro-organisms has an elongated member which advantageously supports a culture medium on each of two opposite flat faces.
  • a container having an open end is provided for the elongated member.
  • a brush mounted in the container contacts the elongated member intermediate its ends.
  • the open end of the container has removable means to seal it.
  • the elongated member is removed from the container and the end thereof brought into contact with the area of the patient suspected of having micro-organsims.
  • the container has a guide member to guide the elongated member and restrict its movement toward the interior surfaces of the container.
  • the brush forms a part of the guide member and the elongated member has a plug adapted to form a seal with the guide member to permit the withdrawal of the elongated member from the container either independently of the guide member or together with the guide member.
  • PAGANO BM M lllII -IIIlllll !IIIII -IIIIIIIII PATENTEDUCT 2 6 I971 ATTORNEYS DEVICE FOR MAKING A CULTURE OF MICRO- ORGANISMS BACKGROUND OF THE INVENTION
  • Devices for making a culture of microorganisms having a culture medium which can be brought directly into contact with an area of a patients body and then returned to a sealed container for growing micro-organisms picked up are known to the art as is seen, for example, in the disclosure of U.S. Pat. No. 3,368,549, issued Feb. 13, 1968. Such devices have as their intended purpose providing office diagnosis within 24 hours without the necessity for a laboratory.
  • a device for making a culture of micro-organisms has an elongated support member which carries a culture medium supported by said member preferably intermediate the ends thereof.
  • a container having an open end is adapted to contain the elongated member and has a brush mounted therein to brush over the inner end of the elongated member and at least a portion of the culture medium on insertion of the elongated member into the container.
  • the container is transparent and has flat surfaces through which the culture medium is viewed.
  • a seal for the open end of the container is provided and it advantageously forms a part of the elongated member.
  • a hollow guide member guides the elongated member within the container to restrict its movement towards the interior surfaces of the container.
  • the brush forms a part of said guide member and the elongated member has a plug engaging the guide member to seal the container.
  • the guide member is removable.
  • FIG. 1 is an elevational view of a prior art device
  • FIG. 2 is an elevational view of a device in accordance with the invention.
  • FIG. 3 is a vertical section taken on the plane indicated by the line 3-3 in FIG. 2;
  • FIG. 4 is an end of view of the guide member and brush of the device of FIG. 2;
  • FIG. 5 is a side elevation of the structure of FIG. 4;
  • FIG. 6 is a top plan view of the elongated support member of the device of FIG. 2 without the culture medium;
  • FIG. 7 is a top plan view, partially broken away, of the member of FIG. 6 with the culture medium added;
  • FIG. 8 is a top plan view, partially broken away, of an alternative elongated member having culture medium extending about the inner end;
  • FIG. 10 is an exploded perspective view of the elongated member and the guide member and brush illustrating the spreading of the inoculum.
  • Prior Art A typical prior art device is shown in FIG. 1.
  • a glass container tube 2 has a threaded cap 4 and contains a stick 6 having a frame end 8 for the support of a solid culture medium 10. It will be noted that the end portion 12 of the culture medium 10 is in contact with the interior surface of the container 2.
  • a device for making a culture of micro-organisms in accordance with the invention has a transparent container 22 of, for example, glass or a plastic such as an acrylic resin such as methyl methacrylate or a polycarbonate resin.
  • an elongated member 24 has an inner end 25 and a recessed portion 26 in opposed substantially that faces (only one being shown). Each recessed portion is intermediate the ends of the elongated member 24 and has multiple small protrusions 30 to facilitate the retention of a body 32 of a solid culture medium in the recessed portions.
  • the culture medium is preferably flush with the adjoining portions the adjacent face 28.
  • elongated member 24 are guided by opposed grooves 36, 36 in hollow guide member 38 which has a cylindrical portion 40 (FIG. 5) provided with ridges 42 which sealingly engage a cylindrical entrance portion 44 of container 22.
  • Guide member 38 is also provided with opposed flats 46, 46 which engage the flat top 48 and the flat bottom 50 of container 22 (FIGS. 9 and 10).
  • the inner end of guide member 38 has a pair of opposed brush members 52, 52 positioned adjacent grooves 36, 36 so as to engage elongated member 24 as it is inserted into container 22.
  • Each brush member 52 has three spaced projections 54 for engaging the elongated member 24.
  • Guide member 38 has a cylindrical entrance portion 56 which is engaged by a plug portion 58 (FIG. 10) of member 24 having a peripheral ridge 60 to seal closed the end of guide member 38.
  • the outer end of elongated member 24 is in the form of handle 64.
  • the guide member will be made of a resilient plastic such as, for example, polyethylene or polypropylene and the brush member 52, 52 will be integral therewith permitting the formation of the guide member and the brush members in a single forming operation from the same material.
  • the culture medium will be selected depending upon the micro-organisms involved. Suitable solid culture mediums are well known to the an. Reference may be had, for example, to the culture mediums disclosed in US. Pat. No. 3,368,549 the disclosure of which is incorporated herein by reference. Agar culture mediums are preferred since their solidity is readily controlled by varying the percent of Agar. Further typical culture mediums are set forth below:
  • a device for making a culture of micro-organisms comprising: an elongated member, a culture medium supported by said member intermediate the ends thereof, a container for the elongated member having an open end, brush means mounted in the container and positioned for contacting the elongated member intermediate its ends so as to brush over at least a portion of the culture medium on insertion of the elongated member into the container and means to seal the open end of the container.
  • the device of claim 1 in which the culture medium extends to and covers at least a portion of the inner end of the elongated member.
  • sealing means includes a plug on the elongated member.
  • a hollow guide member is mounted in the open end of the container to guide the elongated member and restrict its movement towards the interior surfaces of the container.
  • the device claim 1 in which the container is transparent. 10. The device of claim 9 in which the container has a flat portion opposite the elongated member for viewing the culture medium.

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
US848212A 1969-08-07 1969-08-07 Device for making a culture of microorganisms Expired - Lifetime US3616265A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US84821269A 1969-08-07 1969-08-07

Publications (1)

Publication Number Publication Date
US3616265A true US3616265A (en) 1971-10-26

Family

ID=25302670

Family Applications (1)

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US848212A Expired - Lifetime US3616265A (en) 1969-08-07 1969-08-07 Device for making a culture of microorganisms

Country Status (10)

Country Link
US (1) US3616265A (enrdf_load_stackoverflow)
BE (1) BE752895A (enrdf_load_stackoverflow)
CA (1) CA952836A (enrdf_load_stackoverflow)
CH (1) CH540977A (enrdf_load_stackoverflow)
DE (1) DE2034923C3 (enrdf_load_stackoverflow)
ES (1) ES381794A1 (enrdf_load_stackoverflow)
FR (1) FR2057723A5 (enrdf_load_stackoverflow)
GB (1) GB1246070A (enrdf_load_stackoverflow)
LU (1) LU61458A1 (enrdf_load_stackoverflow)
NL (1) NL172172C (enrdf_load_stackoverflow)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2424384A1 (de) * 1973-05-24 1974-12-19 Smithkline Corp Vorrichtung zur herstellung einer mikroorganismenkultur
US3961696A (en) * 1975-10-20 1976-06-08 Smithkline Corporation Device for making a culture of CO2 requiring organisms
US3966552A (en) * 1975-04-14 1976-06-29 Smithkline Corporation Device for making a culture of micro-organisms
US4073695A (en) * 1976-08-11 1978-02-14 Data Packaging Corporation Leighton tube
US4082614A (en) * 1975-12-10 1978-04-04 Bio-Syn, Inc. Means for conveying pathological specimens safely to places of identification
USD249802S (en) 1976-08-11 1978-10-03 Data Packaging Corporation Tissue culture plate
WO1982000834A1 (en) * 1980-09-10 1982-03-18 L Brown Antibiotic testing vessel
US4558520A (en) * 1983-11-30 1985-12-17 Forde Jr George S Self-wiping universal liquid level gauge
US4859586A (en) * 1985-08-26 1989-08-22 Eliahu Eisenberg Device for cultivating bacteria
US4891859A (en) * 1988-12-14 1990-01-09 Napoleon Tremblay Engine oil dip stick indicator wiper
DE10061655A1 (de) * 2000-12-11 2002-06-20 Vermicon Ag In situ-Hybridisierungs-Anordnung zum spezifischen Nachweis von Mikroorganismen
US20050023672A1 (en) * 2003-07-01 2005-02-03 Affymetrix, Inc. Device and method for immersed array packaging and processing
US20100083774A1 (en) * 2008-10-04 2010-04-08 Eppendorf Ag Forensic pipette
US20100083773A1 (en) * 2008-10-04 2010-04-08 Eppendorf Ag Sample carrier
US20100167415A1 (en) * 2008-12-30 2010-07-01 Eppendorf Ag Aliquotting device
US20100193524A1 (en) * 2009-02-05 2010-08-05 Eppendorf Ag Preserving container

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA955163A (en) * 1971-03-16 1974-09-24 Miles Laboratories, Inc. Testing device for microorganisms
GB2141136B (en) * 1983-06-08 1987-03-04 Basildon Moulding Company Limi Dip slide
IT1237781B (it) * 1989-11-21 1993-06-17 Diesse Diagnostica Dispositivo per la conservazione di campioni e per l'isolamento dei germi in detti campioni contenuti per esami microbiologici.

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2424384A1 (de) * 1973-05-24 1974-12-19 Smithkline Corp Vorrichtung zur herstellung einer mikroorganismenkultur
US3862013A (en) * 1973-05-24 1975-01-21 Smithkline Corp Device for making a culture of micro-organisms
US3966552A (en) * 1975-04-14 1976-06-29 Smithkline Corporation Device for making a culture of micro-organisms
DE2615546A1 (de) * 1975-04-14 1976-10-28 Smithkline Corp Vorrichtung zur herstellung einer kultur von mikroorganismen
US3961696A (en) * 1975-10-20 1976-06-08 Smithkline Corporation Device for making a culture of CO2 requiring organisms
US4082614A (en) * 1975-12-10 1978-04-04 Bio-Syn, Inc. Means for conveying pathological specimens safely to places of identification
US4073695A (en) * 1976-08-11 1978-02-14 Data Packaging Corporation Leighton tube
USD249802S (en) 1976-08-11 1978-10-03 Data Packaging Corporation Tissue culture plate
WO1982000834A1 (en) * 1980-09-10 1982-03-18 L Brown Antibiotic testing vessel
US4326028A (en) * 1980-09-10 1982-04-20 Brown Lewis R Antibiotic testing vessel
US4558520A (en) * 1983-11-30 1985-12-17 Forde Jr George S Self-wiping universal liquid level gauge
US4859586A (en) * 1985-08-26 1989-08-22 Eliahu Eisenberg Device for cultivating bacteria
US4891859A (en) * 1988-12-14 1990-01-09 Napoleon Tremblay Engine oil dip stick indicator wiper
DE10061655A1 (de) * 2000-12-11 2002-06-20 Vermicon Ag In situ-Hybridisierungs-Anordnung zum spezifischen Nachweis von Mikroorganismen
WO2002048398A3 (de) * 2000-12-11 2003-05-15 Vermicon Ag In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen
US20050023672A1 (en) * 2003-07-01 2005-02-03 Affymetrix, Inc. Device and method for immersed array packaging and processing
US20100083774A1 (en) * 2008-10-04 2010-04-08 Eppendorf Ag Forensic pipette
US20100083773A1 (en) * 2008-10-04 2010-04-08 Eppendorf Ag Sample carrier
US20100167415A1 (en) * 2008-12-30 2010-07-01 Eppendorf Ag Aliquotting device
US20100193524A1 (en) * 2009-02-05 2010-08-05 Eppendorf Ag Preserving container

Also Published As

Publication number Publication date
DE2034923B2 (de) 1978-11-16
NL7011646A (enrdf_load_stackoverflow) 1971-02-09
BE752895A (fr) 1971-01-04
GB1246070A (en) 1971-09-15
DE2034923C3 (de) 1979-07-12
NL172172B (nl) 1983-02-16
FR2057723A5 (enrdf_load_stackoverflow) 1971-05-21
CH540977A (de) 1973-08-31
ES381794A1 (es) 1972-12-01
DE2034923A1 (de) 1971-02-18
NL172172C (nl) 1983-07-18
CA952836A (en) 1974-08-13
LU61458A1 (enrdf_load_stackoverflow) 1970-10-21

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Owner name: SMITHKLINE BECKMAN CORPORATION, PENNSYLVANIA

Free format text: CHANGE OF NAME;ASSIGNOR:SMITHKLINE CORPORATION;REEL/FRAME:004080/0769

Effective date: 19820304

Owner name: SMITHKLINE BECKMAN CORPORATION

Free format text: CHANGE OF NAME;ASSIGNOR:SMITHKLINE CORPORATION;REEL/FRAME:004080/0769

Effective date: 19820304