US3532605A - Container for biological cultures - Google Patents

Container for biological cultures Download PDF

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Publication number
US3532605A
US3532605A US706317A US3532605DA US3532605A US 3532605 A US3532605 A US 3532605A US 706317 A US706317 A US 706317A US 3532605D A US3532605D A US 3532605DA US 3532605 A US3532605 A US 3532605A
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United States
Prior art keywords
container
wall
mesh
agar
medium
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US706317A
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English (en)
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Jose Vinas Riera
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Individual
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Individual
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices

Definitions

  • ABSTRACT OF THE DISCLOSURE Container for biological cultures having a flat side for receiving solid culture media, a mesh inside for holding the media against said flat side wall while the container is in its upright position and a means for immobilizing said mesh in that position.
  • the present invention refers to a container for biological cultures, especially for blood cultures, designed with a view t improving the formation and examination of bacterial cultures, having a Hat side for receiving the solid culture medium, which in the turned position of the container is covered by the liquid medium, a mesh at least partially embedded in said solid medium and means for immobilising said mesh so that in the upright position of the container the solid culture medium layer does not break away in block.
  • the new type of container which is characterized by the fact that the liquid and solid media of the culture remain at the ready, the former on the bottom of the body of the container and the latter on a larger side wall of the same body, the solid culture medium being composed of a layer of agar-agar which is held against said wall by a mesh of inert material wholly or partially incorporated into the mass of the solid culture medium and giving said medium sufficient rigidity to prevent it from breaking away from the wall or from cracking.
  • One object of the invention is to make the wall which has to support the solid medium somewhat wider than the opposite wall to prevent the medium from breaking away in block.
  • the mesh is wider than the wall to which it is applied, so that, by being slightly curved and under tension, it ensures that the solid medium will remain adhered to the wall.
  • the body of the container has some ridges on the anks of the wall opposite the one to which the solid medium is applied, said ridges having internal grooves into which penetrate and on which rest the ends of the mesh.
  • the means for immobilising the agar-agar is based on the fact that the inert mesh, produced from thermoplastic material, is subjected to a prior thermosetting operation to give it such a shape that, once introduced into the body of the container, it tends to open out and press the agar-agar against the wall of the ask set aside for supporting the solid medi-
  • the edges which vertically ank the wall of the container to which the agar-agar is applied are arranged with a bevel to provide tangential support for the mesh in order to close the dead space that would be left between the solid medium and the curve o fthe mesh at the two corners.
  • the side wall of the body of the container to which the agar-agar is applied has a transverse ridge at the top in order to complete the delimitation of area of spread of this material when it is introduced in a liquid state.
  • the wall on which the agar-agar is applied is made with its faces in perfect mutual parallelism in order to avoid optical aberrrations which render diicult direct, clear observation of the cultures on the agar-agar against the light.
  • FIG. 1 represents a container according to the simplest version of the invention, seen in elevation.
  • FIG 2 is a view of the previous gure seen in cross section taken across the line II-IL
  • FIG. 3 is a view of the container of the previous figure taken across the line III-III.
  • FIG. 4 is a view of the container of FIG. 1, seen in section across the line IV-IV.
  • FIGS. 5 to 8 are similar views to those of FIG. 2 and relate to containers equipped with elements for retaining the solid culture.
  • FIG. 9 is an elevation view across a smaller plane of a container with its neck in an offset position with reference to the centre line.
  • FIG. 10 is a view of the container of the previous figure according to a section across line X-X. f
  • FIG. 11 is a view of the container of FIG. 9 in section taken across a line XI-XI.
  • FIG. 12 is an elevation view across a smaller plane of a container similar to that of FIG. 9, equipped with additional elements for retaining the solid culture.
  • FIG. 13 is a view of the container of the previous figure taken across a line XIII-XIII.
  • FIG. 14 isa view of the container of FIG. 12 taken across along a line XIV-XIV.
  • FIG. 15 is a view of the container of FIG. 12 taken across a line XV-XV.
  • FIG. 16 shows a container similar to that of FIG. 12 giving a diagrammatical view of the limit positions for a rod introduced into said container in order to reach the surface of the culture in the solid medium.
  • a simple container 1 consists of a body with a rectangular base and vertical walls, two larger ones 2, 2', and two smaller ones 3, and a neck 4 suitable for the introduction of the media which compose the culture and to receive a stopper element (not shown).
  • the liquid medium 5 of the culture is to be found in the bottom of the container 1, whilst the solid medium 6, formed by a layer of agar-agar, adheres to one of the larger walls 2, after having been introduced in liquid state with the container being maintained in a horizontal position, with the larger wall 2 placed in the lower position and the larger wall 2 in the upper position, until the agar-agar has acquired a suicient degree of solidity for its stability.
  • the body of the container 1a has a trapezoidal section in which one of the larger walls 2a is wider than the other wall 2a, with the smaller walls 3 being divergent, thus preventing movement of the mesh 7a towards wall 2a.
  • a vertical projection 8 is provided on each smaller wall 3b of the container 1b just beside the larger wall 2b which bears the solid culture 6.
  • the container 1 is provided with a mesh 9 placed by wall 2c which prolongs its width to form some flanks 9a which reach as far as the opposite larger wall 2'c, on which they rest.
  • the container 1 is provided with a mesh 10 of thermoplastic material, larger than the one previously mentioned, which is previously given a curvilinear form so that it is retained inside the container by its tendency to expand against the walls.
  • the mesh 7, 9, 10 may be wholly or partially incorporated in the solid medium, including in this last variation the application of the mesh over the solid medium.
  • the container 1e (FIGS. 12 to l5), according to a preferred embodiment of the invention, has bevelled corners 11 on the vertical edges of the larger face 2e bearing the solid medium, whereby the retaining action of a mesh 9e is accentuated in view of the fact that it is applied tangentially at the edge and reduces the dead space, whereas vertical projections 8a are provided opposite the bevelled corners 11, adapted to receive the ends of the mesh 9e. Also according to the said preferred embodiment of FIGS.
  • an interior ridge 12 is transversally provided inside said container at the top edge of the corresponding larger face 2e which ridge serves to contain the above mentioned material when it enters the container in melted state.
  • FIG. 16 shows a container 1f which indicates by dotted lines the limits of action of a rod 13 of platinum or other material, in order to be able to withdraw samples from the lsolid culture 6.
  • these containers solve the difiiculties expressed at the beginning and facilitate the preparation of cultures, especially haemocultures, by allowing the agar-agar to be spread out over a larger surface than in normal containers, since said surface comprises one whole larger wall of the container; this solid culture medium is stronger since it has a larger adhering surface per unit volume and because of the rigidity conferred to it by the mesh, thereby it is not so open to cracking since the inertia forces are less and since it is held in place in better conditions than in ordinary systems.
  • a container for biological cultures havin-g a substantially polygonal cross-section and an upper neck, and having a stable upright position and a stable turned position substantially at right angles to said upright position
  • the improvement comprising a iiat wall adapted to receive a 'melted solid culture meduim in the turned position of the container, a mesh of inert material inside said container for holding said solid culture medium against said flat wall while the container is in its upright position, said mesh positioned with its greater surface facing said at 'wall and so as to be at least partially incorporated into the mass of the solid culture medium upon addition of said medium, and means for immobilising said mesh when the container is in its upright position.
  • a container for biological cultures in which the lateral dimension of the mesh is greater than that of the Wall adapted to receive the solid culture medium, the side ends of the mesh exercising pressure on the walls of the container, said side ends being the means for immobilising the mesh.
  • a container for biological cultures according to claim 1 in which the Wall adapted to receive the solid medium forms, two sharp angles with they walls adjacent thereto, said adjacent -walls bein-g the means for immobilising the mesh.
  • a container for biological cultures according to claim 1, in which the means for immobilising the mesh for retaining the solid culture medium layer comprises salient ridges provided in the body of the container along two parallel edges of the wall opposite the wall adapted to receive the solid medium, said ridges defining internal furrows adapted to receive the edges of the mesh.
  • a container for biological cultures in which the vertical edges of the Wall of the body of the container to which the solid culture medium layer is applied, are bevelled so as to supply tangential support to the mesh in order to reduce or eliminatethe dead space that would be left in each corner between the medium and the mesh.
  • a container for biological cultures according to claim 1 in which the container has an upper, assymmetrical neck with respect to the body, said neck being offset away from the larger wall which receives the solid culture medium layer in order to facilitate the penetration of a device suitable for reaching the whole of Ithe area of the aforementioned solid culture medium.

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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
US706317A 1967-03-02 1968-02-19 Container for biological cultures Expired - Lifetime US3532605A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES337440A ES337440A1 (es) 1967-03-02 1967-03-02 Recipiente para cultivos biologicos.

Publications (1)

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US3532605A true US3532605A (en) 1970-10-06

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US706317A Expired - Lifetime US3532605A (en) 1967-03-02 1968-02-19 Container for biological cultures

Country Status (5)

Country Link
US (1) US3532605A (de)
DE (2) DE6608387U (de)
ES (1) ES337440A1 (de)
FR (1) FR1559214A (de)
GB (1) GB1170896A (de)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2444081A1 (fr) * 1978-12-14 1980-07-11 Pasteur Institut Methode de culture de microorganismes
US4504547A (en) * 1977-08-08 1985-03-12 Institut Pasteur Process for producing supports for cell cultures and supports so obtained
WO1988000235A1 (en) * 1986-06-24 1988-01-14 Bartal Arie H Apparatus for enhancing cell growth, preservation and transport
WO1990002793A1 (en) * 1988-09-01 1990-03-22 Alena Rogalsky Container for a biological culture
US4952510A (en) * 1987-01-02 1990-08-28 Bio-North, Inc. Apparatus for detecting and culturing microorganisms using a biphasic culture vessel
US5151366A (en) * 1991-05-24 1992-09-29 Invitro Scientific Products, Inc. Cell culture flask
US5272084A (en) * 1991-12-18 1993-12-21 Corning Incorporated Cell culture vessels having interior ridges and method for cultivating cells in same
US5857584A (en) * 1998-01-14 1999-01-12 Taggart; Terry O. Drinking glass liner
EP1400250A1 (de) * 2002-09-18 2004-03-24 InfraServ GmbH & Co. Höchst KG Sterilkolben und dessen Verwendung
US6818438B2 (en) 2002-10-04 2004-11-16 Becton, Dickinson And Company Culture flask
US6987019B1 (en) 2004-09-03 2006-01-17 Vitaly Rogalsky Device for growing cells

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2202158A1 (en) * 1972-10-09 1974-05-03 Inst Pasteur Lille Flask for growing blood analysis cultures - which prevents detachment of gelose layer from flask wall and offers better viewing of cultures
DK377983A (da) * 1982-08-25 1984-02-26 Hana Biolog Inc Trefaset mycoplasmatales kulturapparat og fremgangsmaade
US4912058A (en) * 1989-04-27 1990-03-27 Becton, Dickinson And Company Roller bottle

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2992974A (en) * 1960-04-04 1961-07-18 Allan S Belcove Biological testing device

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2992974A (en) * 1960-04-04 1961-07-18 Allan S Belcove Biological testing device

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4504547A (en) * 1977-08-08 1985-03-12 Institut Pasteur Process for producing supports for cell cultures and supports so obtained
US4537790A (en) * 1977-08-08 1985-08-27 Institut Pasteur Process for growing cell cultures of diploid cells on cell supports
FR2444081A1 (fr) * 1978-12-14 1980-07-11 Pasteur Institut Methode de culture de microorganismes
WO1988000235A1 (en) * 1986-06-24 1988-01-14 Bartal Arie H Apparatus for enhancing cell growth, preservation and transport
US4734373A (en) * 1986-06-24 1988-03-29 Bartal Arie H Apparatus for enhancing cell growth, preservation and transport
US4952510A (en) * 1987-01-02 1990-08-28 Bio-North, Inc. Apparatus for detecting and culturing microorganisms using a biphasic culture vessel
WO1990002793A1 (en) * 1988-09-01 1990-03-22 Alena Rogalsky Container for a biological culture
US5084393A (en) * 1988-09-01 1992-01-28 Alena Rogalsky Container for a biological culture
US5151366A (en) * 1991-05-24 1992-09-29 Invitro Scientific Products, Inc. Cell culture flask
US5272084A (en) * 1991-12-18 1993-12-21 Corning Incorporated Cell culture vessels having interior ridges and method for cultivating cells in same
US5857584A (en) * 1998-01-14 1999-01-12 Taggart; Terry O. Drinking glass liner
EP1400250A1 (de) * 2002-09-18 2004-03-24 InfraServ GmbH & Co. Höchst KG Sterilkolben und dessen Verwendung
US6818438B2 (en) 2002-10-04 2004-11-16 Becton, Dickinson And Company Culture flask
US6987019B1 (en) 2004-09-03 2006-01-17 Vitaly Rogalsky Device for growing cells

Also Published As

Publication number Publication date
DE1667889A1 (de) 1971-07-22
DE6608387U (de) 1971-08-05
ES337440A1 (es) 1968-02-16
FR1559214A (de) 1969-03-07
GB1170896A (en) 1969-11-19

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