US3701717A - Substrate-containing test container for the cultivation and inspection of micro-organisms - Google Patents

Substrate-containing test container for the cultivation and inspection of micro-organisms Download PDF

Info

Publication number
US3701717A
US3701717A US13937A US3701717DA US3701717A US 3701717 A US3701717 A US 3701717A US 13937 A US13937 A US 13937A US 3701717D A US3701717D A US 3701717DA US 3701717 A US3701717 A US 3701717A
Authority
US
United States
Prior art keywords
substrate
tube
cultivation
container
organisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US13937A
Inventor
Helmuth Ingvorsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB4412769A external-priority patent/GB1243750A/en
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of US3701717A publication Critical patent/US3701717A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes

Definitions

  • Substrate-containing test container for the cultivation and inspection of micro-organisms comprising a coating of said substrate on the inner surface of the side walls of said container and an insert means for supporting said coating.
  • This invention relates to a substrate-containing test container and particularly a tubular container for the cultivation and inspection of micro-organisms e.g. bacteria or living cells.
  • Petri dishes It is well known to cultivate micro-organisms in flat circular dishes e.g. Petri dishes, on a substrate such as agar.
  • substrate-containing Petri dishes present large surfaces available for cultivation and therefore the micro organisms growing on a substrate in such Petri dishes can be easily inspected. Therefore, such dishes have found widespread use in clinics, hospitals etc., in which they are used for determining by cultivation the amount and type of bacteria contained in biological fluids.
  • the bacteria cultivated in such Petri dishes are normally studied and counted by means of a microscope.
  • substrate-containing Petri dishes are diflicult to pack in such a manner that they can be sent from one place to another for example by normal post without the risk of breaking such containers during transportation.
  • the object of the invention is to provide a simple and inexpensive substrate-containing container presenting a large free surface available for the cultivation of microorganisms.
  • a substrate-containing container having the inner surface of the side walls coated with a substrate layer.
  • both the bottom and the side walls are coated with said substrate layer.
  • the substrate layer may be applied to the inner surface of the container side walls in several Ways.
  • One of the most simple ways consists in introducing a liquid substrate into the container and rotating the container about its longitudinal axis. When the fluid substrate has been uniformly distributed over the inner surface, the container is cooled to solidify the substrate.
  • the application of the substrate layer can also be effected by introducing a liquid substrate in the container and by moving said container in such a path that the fluid substrate is distributed in an even layer on the inner surfaces. The container is then cooled to solidify the substrate.
  • the substrate layer may also be applied by molding by means of a core which is inserted in the container and is removed after the solidification of the substrate.
  • the substrate layer may be applied by spraying a warm fluid substrate onto the inner surfaces of the container so as to build up a uniform layer on said surfaces.
  • the substrate applied may be solidified by directing a cooling medium for example a stream of cold air onto the exterior side of the container.
  • a further method of applying the substrate on the inner surface of a tube consists in extruding said layer directly onto said surface.
  • the adherence between the tube walls and the substrate layer is insufficient to prevent the substrate layer from being released from the side walls and fall down onto the bottom of the tube. Furthermore, since some micro-organisms liquefy the substrate during their growth it has been found diflicult to maintain a uniform substrate layer on the side walls during the cultivation.
  • This insert means may comprise a number of longitudinal ribs which are connected by spaced transverse ribs so as to form rectangular openings. These ribs are preferably located in such a manner that narrow openings are formed between the side walls and the exterior sides of said ribs.
  • the area of said openings is for Example 1 cm.
  • the insert means can be manufactured in one step and consequently no welding operations are required. After the production of the tubes and the insert means the latter are inserted in the tubes and a substrate layer is applied onto the inner surface of the side walls of the tube.
  • the substrate-containing tube is preferably provided with a screw cap having on its interior side an absorbing pad.
  • This absorbing pad serves to absorb residual liquid present in the substrate-containing tube when a liquid sample, for example urine, has been brought into contact with the substrate and subsequently has been removed from the tube.
  • the insert means preferably comprises an inwardly extending collar provided at its upper end. The exterior side of this collar may be firmly attached to the test tube.
  • the insert means preferably also comprises a bottom plate having a diameter which is equal to that of the tube.
  • FIG. 1 shows a longitudinal sectional view of an embodiment of a substrate-containing container according to the invention
  • FIG. 2 shows a side view of a transparent substratecontaining tube accordance to the invention
  • FIG. 3 shows an enlarged cross sectional view of the substrate-containing tube shown in 'FIG. 1
  • I FIG. 4 shows an enlarged View of an insert means according to the invention.
  • a substrate tube e.g. made from glass or a plastic material, comprises a substrate layer 2 applied onto the inner surfaces of the side walls and the bottom of said tube.
  • the substrate tube shown in FIGS. 2 and 3 comprises a plastic tube 3, having a screw cap 4.
  • the crossing ribs 7 and 8 form openings 10 which at the lower end of the substrate tube are almost quadratic and have an area of 1 cm.
  • the ribs 7 and 8 do not contact the inner surface of the side walls and consequently 4 the liquid substrate medium which is introduced into the tube is permitted to flow in the longitudinal direction as well as transverse thereto so as to form upon cooling a uniform substrate layer 11 firmly anchored to said ribs.
  • a test tube for the cultivation and investigation of micro-organisms having a film of a solid substrate on the inner surface of the tube, and an insert means within the tube and supporting the substrate film, said insert means comprising ribs extending longitudinally of the tube and ribs extending transversely thereto and connecting said longitudinally extending ribs, said ribs being disposed inwardly of the inner surface of the tube so as to form narrow spaces between the outer side of the ribs and the inner surface of the tube, said insert means further comprisinga bottom plate and an annular flange, said plate and flange extending slightly outwardly of said ribs thereby serving to center said insert means in the tube.

Landscapes

  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

SUBSTRATE-CONTAINING TEST CONTAINER FOR THE CULTIVATION AND INSPECTION OF MICRO-ORGANISMS COMPRISING A COATING OF SAID SUBSTRATE ON THE INNER SURFACE OF THE SIDE WALLS OF SAID CONTAINER AND AN INSERT MEANS FOR SUPPORTING SAID COATING.

Description

I YH.INGV ORSEN 3,701,717 SUBS'I'RA'TE-CONTAINING TEST CONTAINER FOR THE CULTIVATION AND INSPECTION OF MICRO-ORGANISMS Filed Feb. 25, 1970 INVENTQR BY 7 I I v ATTORNEY United States Patent 3,701,717 SUBSTRATE-CON TAININ G TEST CONTAINER FOR THE CULTIVATION AND INSPECTION OF MICRO-ORGANISMS Helmuth Ingvorsen, 8 Algade, 4000 Roskilde, Denmark Filed Feb. 25, 1970, Ser. No. 13,937 Claims priority, application Great Britain, Feb. 26, 1969, 10,373/ 69; Sept. 5, 1969, 44,127/69 Int. Cl. C12k 1/10 US. Cl. 195-139 1 Claim ABSTRACT OF THE DISCLOSURE Substrate-containing test container for the cultivation and inspection of micro-organisms comprising a coating of said substrate on the inner surface of the side walls of said container and an insert means for supporting said coating.
BACKGROUND OF THE INVENTION This invention relates to a substrate-containing test container and particularly a tubular container for the cultivation and inspection of micro-organisms e.g. bacteria or living cells.
It is well known to cultivate micro-organisms in flat circular dishes e.g. Petri dishes, on a substrate such as agar. Such substrate-containing Petri dishes present large surfaces available for cultivation and therefore the micro organisms growing on a substrate in such Petri dishes can be easily inspected. Therefore, such dishes have found widespread use in clinics, hospitals etc., in which they are used for determining by cultivation the amount and type of bacteria contained in biological fluids. The bacteria cultivated in such Petri dishes are normally studied and counted by means of a microscope.
However, such substrate-containing Petri dishes are diflicult to pack in such a manner that they can be sent from one place to another for example by normal post without the risk of breaking such containers during transportation.
It has been attempted to use substrate-containing glass tubes which can be packed in a more satisfactory manner. However, the surface area available for the cultivation of micro-organisms is very small in such glass tubes compared to that of Petri dishes. In order to increase the free surface area of the substrate it has been proposed to use tubes in which the portion of the glass tube which forms the basis of the substrate layer is flat and is disposed at an acute angle to the longitudinal axis of the tube.
However, such tubes can be manufactured only by using special molds and consequently, they are more expensive than normal glass tubes.
SUMMARY OF THE INVENTION The object of the invention is to provide a simple and inexpensive substrate-containing container presenting a large free surface available for the cultivation of microorganisms.
According to the invention there is provided a substrate-containing container having the inner surface of the side walls coated with a substrate layer.
In a preferred embodiment of the substrate container of the invention both the bottom and the side walls are coated with said substrate layer.
The substrate layer may be applied to the inner surface of the container side walls in several Ways. One of the most simple ways consists in introducing a liquid substrate into the container and rotating the container about its longitudinal axis. When the fluid substrate has been uniformly distributed over the inner surface, the container is cooled to solidify the substrate.
The application of the substrate layer can also be effected by introducing a liquid substrate in the container and by moving said container in such a path that the fluid substrate is distributed in an even layer on the inner surfaces. The container is then cooled to solidify the substrate.
The substrate layer may also be applied by molding by means of a core which is inserted in the container and is removed after the solidification of the substrate.
Furthermore, the substrate layer may be applied by spraying a warm fluid substrate onto the inner surfaces of the container so as to build up a uniform layer on said surfaces. The substrate applied may be solidified by directing a cooling medium for example a stream of cold air onto the exterior side of the container.
A further method of applying the substrate on the inner surface of a tube consists in extruding said layer directly onto said surface.
In some cases and especially when using tubes prepared from plastic materials the adherence between the tube walls and the substrate layer is insufficient to prevent the substrate layer from being released from the side walls and fall down onto the bottom of the tube. Furthermore, since some micro-organisms liquefy the substrate during their growth it has been found diflicult to maintain a uniform substrate layer on the side walls during the cultivation.
It has been attempted to eliminate these problems by using tubes having small protrusions or ribs on the inner surface of the side walls. However, the manufacture of tubes having such protrusions or ribs which serve to improve the bond between the substrate layer and the tube walls has presented serious problems when the tubes are produced by injection moulding. Thus, it is difiicult to remove the mold parts forming said protrusions or ribs from the tubes. Therefore, it has been necessary to manufacture the tubes in two parts and subsequently to weld these two parts together.
Now it has been found that this complicated production of the tubes can be avoided by using a perforated insert means to be inserted in said tubes so as to support the substrate layer applied on the inner surface of the side walls.
This insert means may comprise a number of longitudinal ribs which are connected by spaced transverse ribs so as to form rectangular openings. These ribs are preferably located in such a manner that narrow openings are formed between the side walls and the exterior sides of said ribs. Thus, when applying a substrate onto the inner surface of the tube the liquid substrate flows into said openings and consequently the substrate layer will be firmly anchored to the insert means, when the liquid substrate has been solidified.
In order to facilitate the counting of the microorganisms grown on the substrate the area of said openings is for Example 1 cm.
The insert means can be manufactured in one step and consequently no welding operations are required. After the production of the tubes and the insert means the latter are inserted in the tubes and a substrate layer is applied onto the inner surface of the side walls of the tube.
The substrate-containing tube is preferably provided with a screw cap having on its interior side an absorbing pad. This absorbing pad serves to absorb residual liquid present in the substrate-containing tube when a liquid sample, for example urine, has been brought into contact with the substrate and subsequently has been removed from the tube.
In order to prevent the absorbing pad from being contacted by the liquid substrate when distributing said substrate in a uniform layer onto the inner surface of the side walls, the insert means preferably comprises an inwardly extending collar provided at its upper end. The exterior side of this collar may be firmly attached to the test tube. In order to obtain an exact mounting of the insert means within the tube the insert means preferably also comprises a bottom plate having a diameter which is equal to that of the tube.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 shows a longitudinal sectional view of an embodiment of a substrate-containing container according to the invention,
FIG. 2 shows a side view of a transparent substratecontaining tube accordance to the invention,
FIG. 3 shows an enlarged cross sectional view of the substrate-containing tube shown in 'FIG. 1, and I FIG. 4 shows an enlarged View of an insert means according to the invention.
DESCRIPTION OF THE PREFERRED EMBODIMENTS In FIG. 1 a substrate tube 1, e.g. made from glass or a plastic material, comprises a substrate layer 2 applied onto the inner surfaces of the side walls and the bottom of said tube.
The substrate tube shown in FIGS. 2 and 3 comprises a plastic tube 3, having a screw cap 4. An insert means 5, shown in detail in FIG. 4 as comprising a bottom plate 6, vertical ribs 7, transverse ribs 8 and a collar 9, is mounted within said tube 3.
The crossing ribs 7 and 8 form openings 10 which at the lower end of the substrate tube are almost quadratic and have an area of 1 cm. The ribs 7 and 8 do not contact the inner surface of the side walls and consequently 4 the liquid substrate medium which is introduced into the tube is permitted to flow in the longitudinal direction as well as transverse thereto so as to form upon cooling a uniform substrate layer 11 firmly anchored to said ribs.
The exact position of the insert means 5 Within the tube 3 is controlled by the bottom plate 6 and an annular flange 12.
I claim:
1. A test tube for the cultivation and investigation of micro-organisms having a film of a solid substrate on the inner surface of the tube, and an insert means within the tube and supporting the substrate film, said insert means comprising ribs extending longitudinally of the tube and ribs extending transversely thereto and connecting said longitudinally extending ribs, said ribs being disposed inwardly of the inner surface of the tube so as to form narrow spaces between the outer side of the ribs and the inner surface of the tube, said insert means further comprisinga bottom plate and an annular flange, said plate and flange extending slightly outwardly of said ribs thereby serving to center said insert means in the tube.
References Cited UNITED STATES PATENTS 2,096,866 9/1937 Thompson 23-259 3,449,210 6/1969 Rohde 195139 3,532,605 9/1970 Riera 195--139 2,533,038 12/1950 Brewer et al. 195--l39 A. LOUIS MONAC-ELL, Primary Examiner R. M. ELLIOTT, Assistant Examiner
US13937A 1969-02-26 1970-02-25 Substrate-containing test container for the cultivation and inspection of micro-organisms Expired - Lifetime US3701717A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1037369 1969-02-26
GB4412769A GB1243750A (en) 1969-02-26 1969-09-05 Substrate-containing test container for the cultivation and inspection of micro-organisms

Publications (1)

Publication Number Publication Date
US3701717A true US3701717A (en) 1972-10-31

Family

ID=26247473

Family Applications (1)

Application Number Title Priority Date Filing Date
US13937A Expired - Lifetime US3701717A (en) 1969-02-26 1970-02-25 Substrate-containing test container for the cultivation and inspection of micro-organisms

Country Status (7)

Country Link
US (1) US3701717A (en)
JP (1) JPS502037B1 (en)
CA (1) CA944707A (en)
DE (1) DE2009108A1 (en)
DK (1) DK128357B (en)
FI (1) FI47379C (en)
FR (1) FR2035725A5 (en)

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3849256A (en) * 1972-01-31 1974-11-19 F Linder Substrate-containing test container
US4690896A (en) * 1984-03-26 1987-09-01 Madaus & Co. Device for the determination of micro-organisms
US4840908A (en) * 1988-06-29 1989-06-20 Becton, Dickinson And Company Culture bottle assembly
US4912048A (en) * 1987-12-21 1990-03-27 Difco Laboratories Fluted culture vessel
US5151366A (en) * 1991-05-24 1992-09-29 Invitro Scientific Products, Inc. Cell culture flask
US5272084A (en) * 1991-12-18 1993-12-21 Corning Incorporated Cell culture vessels having interior ridges and method for cultivating cells in same
US20040191895A1 (en) * 2002-09-20 2004-09-30 Whitley Kenneth W. Roller bottle
US11608486B2 (en) 2015-07-02 2023-03-21 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11613727B2 (en) 2010-10-08 2023-03-28 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11634677B2 (en) 2016-06-07 2023-04-25 Terumo Bct, Inc. Coating a bioreactor in a cell expansion system
US11667881B2 (en) 2014-09-26 2023-06-06 Terumo Bct, Inc. Scheduled feed
US11667876B2 (en) 2013-11-16 2023-06-06 Terumo Bct, Inc. Expanding cells in a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11795432B2 (en) 2014-03-25 2023-10-24 Terumo Bct, Inc. Passive replacement of media
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US12043823B2 (en) 2021-03-23 2024-07-23 Terumo Bct, Inc. Cell capture and expansion

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52137444U (en) * 1976-04-13 1977-10-19
DE19607428C2 (en) * 1996-02-28 1999-01-28 Daimler Benz Ag Interior trim part made of plastic

Cited By (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3849256A (en) * 1972-01-31 1974-11-19 F Linder Substrate-containing test container
US4690896A (en) * 1984-03-26 1987-09-01 Madaus & Co. Device for the determination of micro-organisms
US4912048A (en) * 1987-12-21 1990-03-27 Difco Laboratories Fluted culture vessel
US4840908A (en) * 1988-06-29 1989-06-20 Becton, Dickinson And Company Culture bottle assembly
US5151366A (en) * 1991-05-24 1992-09-29 Invitro Scientific Products, Inc. Cell culture flask
US5272084A (en) * 1991-12-18 1993-12-21 Corning Incorporated Cell culture vessels having interior ridges and method for cultivating cells in same
US20040191895A1 (en) * 2002-09-20 2004-09-30 Whitley Kenneth W. Roller bottle
US7449331B2 (en) 2002-09-20 2008-11-11 Whitley Kenneth W Roller bottle
US11746319B2 (en) 2010-10-08 2023-09-05 Terumo Bct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11773363B2 (en) 2010-10-08 2023-10-03 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11613727B2 (en) 2010-10-08 2023-03-28 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11667876B2 (en) 2013-11-16 2023-06-06 Terumo Bct, Inc. Expanding cells in a bioreactor
US11708554B2 (en) 2013-11-16 2023-07-25 Terumo Bct, Inc. Expanding cells in a bioreactor
US11795432B2 (en) 2014-03-25 2023-10-24 Terumo Bct, Inc. Passive replacement of media
US11667881B2 (en) 2014-09-26 2023-06-06 Terumo Bct, Inc. Scheduled feed
US12065637B2 (en) 2014-09-26 2024-08-20 Terumo Bct, Inc. Scheduled feed
US11608486B2 (en) 2015-07-02 2023-03-21 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11634677B2 (en) 2016-06-07 2023-04-25 Terumo Bct, Inc. Coating a bioreactor in a cell expansion system
US11999929B2 (en) 2016-06-07 2024-06-04 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US12077739B2 (en) 2016-06-07 2024-09-03 Terumo Bct, Inc. Coating a bioreactor in a cell expansion system
US11702634B2 (en) 2017-03-31 2023-07-18 Terumo Bct, Inc. Expanding cells in a bioreactor
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US12043823B2 (en) 2021-03-23 2024-07-23 Terumo Bct, Inc. Cell capture and expansion

Also Published As

Publication number Publication date
FI47379B (en) 1973-07-31
JPS502037B1 (en) 1975-01-23
CA944707A (en) 1974-04-02
DK128357B (en) 1974-04-16
FR2035725A5 (en) 1970-12-18
FI47379C (en) 1973-11-12
DE2009108A1 (en) 1970-09-17

Similar Documents

Publication Publication Date Title
US3701717A (en) Substrate-containing test container for the cultivation and inspection of micro-organisms
US5026649A (en) Apparatus for growing tissue cultures in vitro
US4912058A (en) Roller bottle
EP3317394B1 (en) Device for propagating microtissues
US4912037A (en) Container for micro-organism culture media
US4939151A (en) Adherent cell culture flask
US7888111B2 (en) Cell culture vessel, production process thereof and cultured cell
US20200131461A1 (en) Cell culture vessel
US20200181552A1 (en) Handling features for microcavity cell culture vessel
US20010055803A1 (en) Cell cultivating flask and method for using the cell cultivating flask
US3630849A (en) Surface micro-organism contamination assays
JPH0330664A (en) Culture medium package and method
US3740321A (en) Cell propagator
DE2338546C2 (en) Device for the propagation of tissue culture cells
US5583037A (en) Trans-membrane co-culture insert and method for using
US4121976A (en) Culturing bottle and method of making
KR880001480B1 (en) Container of prepared nutrient substrate carrier
US4715911A (en) Method for the production of a plastic container
US4358539A (en) Throw-away subculturing device
DE2236240B2 (en) Device for growing tissue cells and microorganisms
GB2182144A (en) Culture vessel
US4840908A (en) Culture bottle assembly
KR880000455B1 (en) Device for the determination of micro-organisms
IL34569A (en) Apparatus for the growth of living cells on a large scale
JPH01137964A (en) Culture vessel