US20220040180A1 - Kits and methods for treating cancers - Google Patents

Kits and methods for treating cancers Download PDF

Info

Publication number
US20220040180A1
US20220040180A1 US17/275,290 US201917275290A US2022040180A1 US 20220040180 A1 US20220040180 A1 US 20220040180A1 US 201917275290 A US201917275290 A US 201917275290A US 2022040180 A1 US2022040180 A1 US 2022040180A1
Authority
US
United States
Prior art keywords
crystalline form
compound
kit
cancer
chemical formula
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US17/275,290
Other languages
English (en)
Inventor
Guru Reddy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spectrum Pharmaceuticals Inc
Original Assignee
Spectrum Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spectrum Pharmaceuticals Inc filed Critical Spectrum Pharmaceuticals Inc
Priority to US17/275,290 priority Critical patent/US20220040180A1/en
Assigned to SPECTRUM PHARMACEUTICALS reassignment SPECTRUM PHARMACEUTICALS ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: REDDY, GURU
Publication of US20220040180A1 publication Critical patent/US20220040180A1/en
Assigned to SLR INVESTMENT CORP., AS COLLATERAL AGENT reassignment SLR INVESTMENT CORP., AS COLLATERAL AGENT SECURITY INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: ALLOS THERAPEUTICS, INC., SPECTRUM PHARMACEUTICALS, INC., TALON THERAPEUTICS, INC.
Assigned to TALON THERAPEUTICS, INC., ALLOS THERAPEUTICS, INC., SPECTRUM PHARMACEUTICALS, INC. reassignment TALON THERAPEUTICS, INC. TERMINATION AND RELEASE OF INTELLECTUAL PROPERTY SECURITY AGREEMENT RECORDED AT REEL 061179/FRAME 0202 Assignors: SLR INVESTMENT CORP.
Abandoned legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/28—Compounds containing heavy metals
    • A61K31/282—Platinum compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/337—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having four-membered rings, e.g. taxol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A61K31/403—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with carbocyclic rings, e.g. carbazole
    • A61K31/404—Indoles, e.g. pindolol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
    • A61K31/4439—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/475—Quinolines; Isoquinolines having an indole ring, e.g. yohimbine, reserpine, strychnine, vinblastine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/513—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim having oxo groups directly attached to the heterocyclic ring, e.g. cytosine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/517—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with carbocyclic ring systems, e.g. quinazoline, perimidine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A61K31/52—Purines, e.g. adenine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/655—Azo (—N=N—), diazo (=N2), azoxy (>N—O—N< or N(=O)—N<), azido (—N3) or diazoamino (—N=N—N<) compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
    • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
    • A61K31/7068—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
    • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
    • A61K31/7068—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid
    • A61K31/7072—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid having two oxo groups directly attached to the pyrimidine ring, e.g. uridine, uridylic acid, thymidine, zidovudine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
    • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
    • A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/243—Platinum; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/177—Receptors; Cell surface antigens; Cell surface determinants
    • A61K38/179—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/3955—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against proteinaceous materials, e.g. enzymes, hormones, lymphokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/13—Crystalline forms, e.g. polymorphs

Definitions

  • This patent document relates to a kit containing crystalline forms of a quinazoline compound and its hydrochloride salt forms. More particularly, the crystalline forms are derived from 1-(4-(4-(3,4-dichloro-2-fluorophenylamino)-7-methoxyquinazolin-6-yloxy)piperidin-1-yl)prop-2-en-1-one.
  • the compound of chemical formula (1) prepared in the above cited patents is generally prepared in the form of amorphous solid or incomplete crystal which is less well suited for large-scale pharmaceutical processing, and there is no description with regard to the preparation of specific crystalline forms.
  • the compound of chemical formula (1) prepared by the above cited patents has a disadvantage in that the solubility in water is very low.
  • the compound of formula (I) prepared by the cited patents unavailable in the form of uniform crystals, meeting physicochemical stability standards required for pharmaceuticals can be troublesome.
  • An aspect of this patent document provides a kit for treating cancer containing a crystalline form derived from a compound of Chemical formula (1):
  • a dihydrate (2H 2 O) of the compound of Chemical Formula (1) and the crystalline form has an X-ray powder diffraction (XRPD) pattern comprising peaks at diffraction angle 2 ⁇ values of 9.4° ⁇ 0.2°, 13.0° ⁇ 0.2° and 18.5° ⁇ 0.2° when irradiated with a Cu-K ⁇ light source (polymorph-a);
  • XRPD X-ray powder diffraction
  • an anhydrous monohydrochloride of the compound of Chemical Formula (1) having an XRPD pattern comprising peaks at diffraction angle 2 values ⁇ of 9.5° ⁇ 0.2°, 23.0° ⁇ 0.2°, 23.2° ⁇ 0.2° and 23.5° ⁇ 0.2° when irradiated with a Cu-K ⁇ light source (polymorph-e).
  • the chemical purity of the crystalline form is greater than 85%, 90%, 95% or 99%.
  • the crystalline form is as described in (a), and wherein the crystalline form has a 13 C solid state nuclear magnetic resonance (ssNMR) spectrum comprising peaks at the following chemical shifts: 147.7 ⁇ 0.5, 156.2 ⁇ 0.5 and 165.4 ⁇ 0.5 ppm.
  • ssNMR 13 C solid state nuclear magnetic resonance
  • the crystalline form is as described in (b), and wherein the crystalline form has a 13 C ssNMR spectrum comprising peaks at the following chemical shifts: 54.3 ⁇ 0.5, 127.3 ⁇ 0.5, 146.9 ⁇ 0.5 and 156.7 ⁇ 0.5 ppm.
  • the crystalline form is as described in (c), and wherein the crystalline form has a 13 C ssNMR spectrum comprising peaks at the following chemical shifts: 129.2 ⁇ 0.5, 153.1 ⁇ 0.5, 156.7 ⁇ 0.5 and 165.2 ⁇ 0.5 ppm.
  • the crystalline form is as described in (d), and wherein the crystalline form has a 13 C ssNMR spectrum comprising peaks at the following chemical shifts: 145.8 ⁇ 0.5, 157.8 ⁇ 0.5 and 164.5 ⁇ 0.5 ppm.
  • the crystalline form is as described in (e), and wherein the crystalline form has a 13 C ssNMR spectrum comprising peaks at the following chemical shifts: 146.9 ⁇ 0.5, 158.7 ⁇ 0.5 and 163.0 ⁇ 0.5 ppm.
  • the kit also includes at least one cytotoxic agent and/or at least one molecularly targeted agent, as the active ingredient, wherein the at least one cytotoxic agent is selected from the group consisting of taxanes, base analogs, platinum-based antineoplastic drugs and vinca alkaloids, and wherein the at least one molecularly targeted agent is selected from the group consisting of at least one epidermal growth factor receptor (EGFR) family inhibitor.
  • EGFR epidermal growth factor receptor
  • the kit includes the EGFR family inhibitor is an anti-EGFR family antibody.
  • the anti-EGFR family antibody selected from the group consisting of trastuzumab, T-DM1, margetuximab cetuximab, matuzumab, panitumumab, necitumumab, and pertuzumab.
  • the kit includes the taxane.
  • the taxane is selected from the group consisting of paclitaxel, docetaxel and cabazitaxel.
  • the kit includes the base analog.
  • the base analog is selected from the group consisting of 5-fluorouracil, 6-mercaptopurine, capecitabine, gemcitabine, pemetrexed, methotrexate, cladribine, cytarabine, doxifludine, floxuridine, fludarabine and decarbazine.
  • the kit includes the platinum-based antineoplastic drug.
  • the platinum-based antineoplastic drug is selected from the group consisting of cisplatin, carboplatin, dicycloplatin, eptaplatin, lobaplatin, miriplatin, nedaplatin, oxaliplatin, picoplatin, and satraplatin.
  • the kit includes the vinca alkaloid.
  • the vinca alkaloid is selected from the group consisting of vinblastine, vincristine, vinflunine, vinorelbine, vincaminol, vinburnine,incidine and vindesine.
  • the kit includes the EGFR family inhibitor.
  • the mTOR inhibitor selected from the group consisting of zotarolimus, umirolimus, temsirolimus, sirolimus, sirolimus NanoCrystal, sirolimus TransDerm, sirolimus-PNP, everolimus, biolimus A9, ridaforolimus, rapamycin, TCD-10023, DE-109, MS-R001, MS-R002, MS-R003, Perceiva, XL-765, quinacrine, PKI-587, PF-04691502, GDC-0980, dactolisib, CC-223, PWT-33597, P-7170, LY-3023414, INK-128, GDC-0084, DS-7423, DS-3078, CC-115, CBLC-137, AZD-2014, X-480, X-414, EC-0371, VS-5584, PQR-
  • the kit includes the cytotoxic agent.
  • cytotoxic agent is selected from the group consisting of paclitaxel, cisplatin, 5-fluorouracil, vinorelbine and any combinations thereof.
  • the kit includes the molecularly targeted agent.
  • the molecularly targeted agent is selected from the group consisting of cetuximab, trastuzumab, T-DM1 and any combinations thereof.
  • Another aspect of this patent document provides a method of treating a neoplasm in a subject comprising administering to a subject in need thereof a crystalline form described herein.
  • the method further includes administering to the subject at least one cytotoxic agent and/or at least one molecularly targeted agent, as the active ingredient, wherein the at least one cytotoxic agent is selected from the group consisting of taxanes, base analogs, platinum-based antineoplastic drugs and vinca alkaloids, and wherein the at least one molecularly targeted agent is selected from the group consisting of at least one epidermal growth factor receptor (EGFR) family inhibitor.
  • EGFR epidermal growth factor receptor
  • FIGS. 1A, 1B, 1C, 1D and 1E show the X-ray powder diffraction (XRPD) spectrums of the compound of chemical formula (1) and its crystalline hydrochloride salt form according to the example:
  • FIG. 1A shows the XRPD for the crystalline form prepared in Example 1:
  • FIG. 1B shows XRPD for the crystalline form prepared in Example 2;
  • FIG. 1C shows XRPD for the crystalline form prepared in Example 3;
  • FIG. 1D shows XRPD for the crystalline form prepared in Example 4;
  • FIG. 1E shows XRPD for the crystalline form prepared in Example 5.
  • FIGS. 1F and 1G show the XRPD spectrums of the compound of chemical formula (1) and its amorphous hydrochloride salt form according to the comparative example; FIG. 1F shows XRPD for the amorphous form prepared in Example 6; and FIG. 1G shows XRPD for the compound of chemical formula (1) prepared in Reference Example.
  • FIGS. 2A, 2B, 2C, 2D and 2E show the solid state nuclear magnetic resonance (ssNMR) spectrums of the compound of chemical formula (1) and its crystalline hydrochloride salt form according to the example;
  • FIG. 2A shows the ssNMR for the crystalline form prepared in Example 1;
  • FIG. 2B shows ssNMR for the crystalline form prepared in Example 2;
  • FIG. 2C shows ssNMR for the crystalline form prepared in Example 3:
  • FIG. 2D shows ssNMR for the crystalline form prepared in Example 4;
  • FIG. 2E shows ssNMR for the crystalline form prepared in Example 5.
  • FIGS. 2F and 2G show the ssNMR spectrums of the compound of chemical formula (1) and its amorphous hydrochloride salt form according to the comparative example;
  • FIG. 2F shows DVS for the amorphous form prepared in Example 6;
  • FIG. 2G shows ssNMR for the compound of chemical formula (1) prepared in Reference Example.
  • FIGS. 3A, 3B, 3C, 3D and 3E show the Differential Scanning Calorimetry (DSC) graphs of the compound of chemical formula (1) and its crystalline hydrochloride salt form according to the example;
  • FIG. 3A shows the DSC for the crystalline form prepared in Example 1;
  • FIG. 3B shows DSC for the crystalline form prepared in Example 2;
  • FIG. 3C shows DSC for the crystalline form prepared in Example 3;
  • FIG. 3D shows DSC for the crystalline form prepared in Example 4;
  • FIG. 3E shows DSC for the crystalline form prepared in Example 5.
  • FIGS. 4A, 4B, 4C, 4D and 4E show the dynamic vapor sorption (DVS) graphs of the compound of chemical formula (1) and its crystalline hydrochloride salt form according to the example;
  • FIG. 4A shows the DVS for the crystalline form prepared in Example 1;
  • FIG. 4B shows DVS for the crystalline form prepared in Example 2;
  • FIG. shows DVS for the crystalline form prepared in Example 3;
  • FIG. 4D shows DVS for the crystalline form prepared in Example 4;
  • FIG. 4E shows DVS for the crystalline form prepared in Example 5.
  • FIGS. 4F and 4G show the DVS graphs of the compound of chemical formula (1) and its amorphous hydrochloride salt form according to the comparative example;
  • FIG. 4F shows ssNMR for the amorphous form prepared in Example 6;
  • FIG. 4G shows DVS for the compound of chemical formula (1) prepared in Reference Example.
  • the term “about” as used herein means within 5%, preferably between 1% and 2% of a given value or range.
  • the expression “about 10%” refers to 9.5% to 10.5%, preferably 9.8% to 10.2%.
  • the expression “about 100° C.” refers to 95° C. to 105° C., preferably 98° C. to 102° C.
  • the term “chemical purity” refers to the weight % that is the specified chemical entity, including specified polymorph form.
  • a crystalline dihydrate (2H 2 O) of the compound of Formula (1) is characterized as having greater than 95% chemical purity, that means that greater than 95% by weight of the substance is the crystalline dihydrate (2H 2 O) of the compound of Formula (1) and less than 5% by weight of any other compound including other anhydrous forms and/or polymorphs.
  • a particular monohydrochloride monohydrate crystalline form (1HCl.1H 2 O) of the compound of Formula (1) is characterized as having greater than 95% chemical purity
  • this particular crystalline form is more than 95% by weight among all forms (including for example crystalline or non-crystalline form, salt form or salt-free form, hydrate or anhydrous form) of the compound of Formula (1) in the same composition or kit.
  • the term “derived” in this context refers to forming a desirable crystalline form (e.g. an hydrous form, a hydrate form, or a pharmaceutically acceptable salt) of the compound of Formula (1) without changing the chemical structure of the compound.
  • the term “poziotinib” as used herein refers to any of the crystalline forms of the compound of formula (1).
  • the peak value of the diffraction angle (28) at the X-ray powder diffraction (XRPD) spectrum reported in this patent document has preferably the experimental error of ⁇ 0.5%, more preferably ⁇ 0.2% which is typically observable in the art.
  • the chemical shift in the solid state nuclear magnetic resonance spectrum reported in this patent document will be preferably interpreted as within ⁇ 0.5 ppm, more preferably as within ⁇ 0.2%.
  • the compound of chemical formula (1) above may be prepared according to the general procedure described in Korean Patent No. 1,013,319 and U.S. Pat. No. 8,003,658, all of which are incorporated herein by reference in their entirety.
  • the compound of chemical formula (1) described in the above documents is a poorly soluble compound which is amorphous and has a solubility in water of less than 1.0 ⁇ g/mL.
  • salts aids in the solubilization of the water-insoluble drug substance.
  • these salts must have various physicochemical properties such as reproducibility of the preparation of specific crystalline form, high crystallinity, stability of crystalline form, chemical stability, and non-hygroscopicity, etc. which are pharmacologically required.
  • various salts of the compound of chemical formula (1) were prepared using various acids and solvents according to various conditions and procedures, and their physicochemical properties were evaluated.
  • the crystalline forms of the various forms of the hydrochloride salt of the compound of chemical formula (1) are the most excellent in terms of various physicochemical properties such as reproducibility of the preparation of specific crystalline form, high crystallinity, stability of crystalline form, chemical stability, and non-hygroscopicity, etc. which are pharmacologically required.
  • the salt of the compound of chemical formula (1) may be prepared in crystalline form, amorphous form or a mixture thereof, but it is preferable that the salt is in crystalline form.
  • the crystalline hydrochloride salt form of the compound of chemical formula (1) is preferable in that it has excellent stability and physicochemical properties that are easy to formulate.
  • the compound of chemical formula (1) may be in the form of various crystalline forms, for example, its crystalline dihydrate (2H 2 O) form and crystalline anhydrous form.
  • the compound of chemical formula (1) may be in the form of various crystalline hydrochloride salts, for example, crystalline monohydrochloride monohydrate (1HCl.1H 2 O) form and their crystalline anhydrous monohydrochloride salt (1HCl) form.
  • the crystalline anhydrous monohydrochloride salt form was the most excellent in solubility in water, and as a result of examining in Test Example 2, it may be advantageous in terms of non-hygroscopicity and stability, and thus may be desirable as a useful active ingredient in pharmaceutical compositions.
  • this patent document provides the crystalline dihydrate (2H 2 O) form of the compound of chemical formula (1).
  • the above crystalline form may have chemical shifts (ppm ⁇ 0.5 ppm) of 147.7, 156.2, and 165.4 ppm in the 13 C CP/MAS TOSS solid state nuclear magnetic resonance (cross polarization/magic angle spinning total suppression of sidebands solid state nuclear magnetic resonance, ssNMR) spectrum.
  • the above crystalline form may have a moisture content (theoretical moisture content of 6.83%) of about 7.5%, a condensation temperature of about 117-122° C. and a melting point of about 190-195° C.
  • the above crystalline form can be measured to have hygroscopic degree of about 2% to 5% in the relative humidity range of 0-90%, as measured by the DVS.
  • the crystalline anhydrous Form I of the compound of chemical formula (1) has the XRPD spectrum including peaks at the diffraction angle (2 ⁇ 0.2°) of 6.0, 10.6, 10.9, 12.1, 16.0, 17.5, 18.3, 19.2, 20.3, 22.7, 23.7 and 26.3° when irradiated with a Cu-K ⁇ light source. These peaks may be peaks with relative intensities of about 10% to 20% or more.
  • the above crystalline form may have chemical shifts (ppm ⁇ 0.5 ppm) of 54.3, 127.3, 146.9 and 156.7 ppm in the 13 C CP/MAS TOSS solid state nuclear magnetic resonance (ssNMR) spectrum.
  • the above crystalline form may have an endothermic peak of the lowest point at about 191° C. when running from a starting point of about 186° C., as measured by the DSC (10° C./min).
  • the above crystalline form may have a moisture content of about 0.3% and a melting point of about 183-185° C.
  • the above crystalline form may have an endothermic peak of the lowest point at about 185° C. when running from a starting point of about 181° C., as measured by the DSC (10° C./min).
  • the above crystalline form can be measured to have very low hygroscopic degree in a relative humidity range of 0-90%, as measured by the DVS.
  • the above crystalline form may have chemical shifts (ppm ⁇ 0.5 ppm) of 145.8, 157.8 and 164.5 ppm in the 13 C CP/MAS TOSS solid state nuclear magnetic resonance (ssNMR) spectrum.
  • the above crystalline form may have an endothermic peak of the lowest point at about 151° C. and an endothermic peak at about 178° C. when running from a starting point of about 127° C., as measured by the DSC (10° C./min).
  • the above crystalline form may have a moisture content of about 3.2% (theoretical moisture content 3.30%) and a melting point of about 187-193° C.
  • the crystalline anhydrous monohydrochloride salt (1HCl) form of the compound of chemical formula (1) may have the XRPD spectrum including peaks at the diffraction angle (2 ⁇ 0.2°) of 9.5, 12.3, 13.0, 13.5, 14.2, 21.4, 23.0, 23.2, 23.5, 27.2 and 27.5° when irradiated with a Cu-K ⁇ light source. These peaks may be peaks with relative intensities of about 10% to 20% or more.
  • the above crystalline form may have an endothermic peak of the lowest point at about 230° C. when running from a starting point of about 201° C., as measured by the DSC (10° C./min).
  • the above crystalline form may have a moisture content of about 0.1% and a melting point of about 238-243° C.
  • a process for the preparation crystalline hydrochloride salt forms (hydrate or anhydrous) of the compound of chemical formula (1) is also provided.
  • the process involves:
  • crystalline forms (hydrate or anhydrous) of the compound of chemical formula (1) according to this patent document and its crystalline hydrochloride salt forms (hydrate or anhydrous) do not require particular storage conditions and can be stably maintained for a long period of time. Capable of meeting the physicochemical properties required for pharmaceuticals including excellent solubility in water, they are readily usable in the manufacture of pharmaceutical compositions containing these as active ingredients.
  • the crystalline forms (hydrate or anhydrous) of the compound of chemical formula (1) according to this patent document have a high chemical purity.
  • the chemical purity is greater than about 75%, greater than about 80%, greater than about 85%, greater than about 90%, greater than about 95%, or greater than about 99%.
  • the compound of chemical formula (1) has an antiproliferative activity such as anticancer activity and has an activity of selectively and effectively inhibiting the growth and drug resistance of cancer cells induced by tyrosine kinase or its variants.
  • the crystalline form of the compound of chemical formula (1) and its hydrochloride salt can be used to produce a pharmaceutical composition for the treatment or prevention of various solid cancers, such as cancers or tumors, particularly lung cancer, breast cancer, etc. caused by tyrosine kinase or its variants.
  • the dosage of the crystalline form of the compound of chemical formula (1) and hydrochloride salts thereof may vary depending on the subject to be treated, the severity of the disease or condition, the rate of administration and the judgment of the prescribing physician, but they can be usually administered to an individual as an active ingredient in an amount of 1 to 2,000 mg per kg of body weight 70 kg of free base of the compound of chemical formula (1), preferably 5 to 1,000 mg based on the compound of chemical formula (1), usually on a schedule of one to four times a day, or on/off schedule, via an oral or parenteral route.
  • dosage less than the above-mentioned ranges may be more suitable, dosage more than the above-mentioned ranges may be also used without causing harmful side effects, and in the case of the higher dosage, it is administered in divided doses several times per day.
  • composition according to this patent document may be formulated according to the conventional method and may be prepared in various oral dosage forms such as tablets, pills, powders, capsules, syrups, emulsions or micro-emulsions, etc. or in parenteral dosage forms such as intramuscular, intravenous or subcutaneous administration.
  • examples of the carrier include water, saline solution, aqueous glucose solution, aqueous pseudosugar solution, alcohols, glycols, ethers (for example, polyethylene glycol 400), oils, fatty acids, fatty acid esters, glycerides, surfactants, suspending agents, emulsifiers and the like.
  • the pharmaceutical composition further includes a non-metallic salt lubricant selected from the group consisting of glyceryl behenate, glyceryl palmitostearate, glyceryl monostearate, glyceryl trimyristate, glyceryl tristearate, sucrose fatty acid ester, palmitic acid, palmitoyl alcohol, stearic acid, stearyl alcohol, fumaric acid, polyethyleneglycol 4000, polyethyleneglycol 6000, polytetrafluoroethylene, starch, talc, hydrogenated castor oil, mineral oil, hydrogenated vegetable oil, silicon dioxide, and any combination thereof.
  • a non-metallic salt lubricant selected from the group consisting of glyceryl behenate, glyceryl palmitostearate, glyceryl monostearate, glyceryl trimyristate, glyceryl tristearate, sucrose fatty acid ester, palmitic acid, palmitoyl alcohol,
  • the pharmaceutical composition further includes a metallic salt lubricant.
  • a metallic salt lubricant include magnesium stearate, magnesium silicate, stearic acid, and calcium stearate.
  • a related aspect of the invention includes a kit for treating cancer including the crystalline form described herein or a pharmaceutical composition thereof.
  • the kit or the pharmaceutical composition can contain an additional cytotoxic agent or a molecularly targeted agent.
  • more than about 50%, more than about 60%, more than about 70%, more than about 80%, more than about 90%, more than about 95%, or more than about 99% by weight of the compound of Formula (1) in the kit or pharmaceutical composition is in the form of a crystalline form described herein (polymorph-a, polymorph-b, polymorph-c, polymorph-d, or polymorph-e).
  • more than about 50%, more than about 60%, more than about 70%, more than about 80%, more than about 90%, more than about 95%, or more than about 99% by weight of the compound of Formula (1) in the kit or composition are in the form of two or more of crystalline forms selected from polymorph-a, polymorph-b, polymorph-c, polymorph-d, and polymorph-e.
  • the crystalline form described herein or a pharmaceutical composition thereof and the additional cytotoxic agent can be administered sequentially or simultaneously, depending on the specific conditions of the subject.
  • the kit will contain poziotinib and the other constituent agent(s) or pharmaceutically acceptable salts or solvates thereof, in separate pharmaceutical compositions.
  • the combination kit can comprise poziotinib and the other constituent drug(s) or pharmaceutically acceptable salts or solvates thereof in separate pharmaceutical compositions in a single package or in separate pharmaceutical compositions in separate packages.
  • a cytotoxic agent refers to an agent that has a cytotoxic effect on a cell.
  • a cytotoxic effect refers to the depletion, elimination and/or the killing of a target cells (i.e., tumor cells).
  • the cytotoxic agent may be at least one selected from the group consisting of an antimetabolite, a mitotic inhibitor, alkylating agent, a platinum-based antineoplastic drug, an mTOR inhibitor, a VEGF inhibitor, an aromatase inhibitor and a CDK4/6 inhibitor.
  • the kit or combination of agents may include at least two cytotoxic agents.
  • the combination may include at least 2, at least 3, or at least 4 selected from the group consisting of an antimetabolite, a mitotic inhibitor, alkylating agent, a platinum-based antineoplastic drug, an mTOR inhibitor, a VEGF inhibitor, an aromatase inhibitor, a CDK4/6 inhibitor and all of them.
  • the antimetabolite may be a drug that inhibits DNA synthesis in cells by suppressing formation of purines or pyrimidines, which are bases of a nucleotide.
  • the antimetabolite may be selected from the group consisting of Capecitabine, 5-Fluorouracil, Gemcitabine, Pemetrexed, Methotrexate, 6-Mercaptopurine, Cladribine, Cytarabine, Doxifludine, Floxuridine, Fludarabine, Hydroxycarbamide, decarbazine, hydroxyurea, and asparaginase.
  • the mitotic inhibitor may be a microtubule-destabilizing agent, a microtubule-stabilizing agent, or a combination thereof.
  • the mitotic inhibitor may be taxane, vinca alkaloid, epothilone, or a combination thereof.
  • the mitotic inhibitor may be selected from BT-062, HMN-214, eribulin mesylate, vindesine, EC-1069, EC-1456, EC-531, vintafolide, 2-methoxyestradiol, GTx-230, trastuzumab emtansine, crolibulin, D1302A-maytansinoid conjugates IMGN-529, lorvotuzumab mertansine, SAR-3419, SAR-566658, IMP-03138, topotecan/vincristine combinations, BPH-8, fosbretabulin tromethamine, estramustine phosphate sodium, vincristine, vinflunine, vinorelbine, RX-21101, cabazitaxel, STA-9584, vinblastine, epothilone A, patupilone, ixabepilone, Epothilone D, paclitaxel, docetaxel, DJ
  • an “alkylating agent” is a substance that adds one or more alkyl groups (CnHm, where n and m are integers) to a nucleic acid.
  • an alkylating agent is selected from the group consisting of nitrogen mustards, nitrosoureas, alkyl sulfonates, triazines, ethylenimines, and combinations thereof.
  • nitrogen mustards include mechlorethamine, chlorambucil, cyclophosphamide, bendamustine, ifosfamide, melphalan, melphalan flufenamide, and pharmaceutically acceptable salts thereof.
  • Non-limiting examples of nitrosoureas include streptozocin, carmustine, lomustine, and pharmaceutically acceptable salts thereof.
  • Non-limiting examples of alkyl sulfonates include busulfan and pharmaceutically acceptable salts thereof.
  • Non-limiting examples of triazines include dacarbazine, temozolomide, and pharmaceutically acceptable salts thereof.
  • Non-limiting examples of ethylenimines include thiotepa, altretamine, and pharmaceutically acceptable salts thereof.
  • Other alkylating agents include ProLindac, Ac-225 BC-8, ALF-2111, trofosfamide, MDX-1203, thioureidobutyronitrile, mitobronitol, mitolactol, nimustine, glufosfamide, HuMax-TAC and PBD ADC combinations, BP-C1, treosulfan, nifurtimox, improsulfan tosilate, ranimustine, ND-01, HH-1, 22P1 G cells and ifosfamide combinations, estramustine phosphate, prednimustine, lurbinectedin, trabectedin, altreatamine, SGN-CD33A, fotemustine, nedaplatin, heptaplatin, apaziquone, SG-2000, TLK-5
  • the platinum-based antineoplastic drug may be for example, Cisplatin, Carboplatin, Dicycloplatin, Eptaplatin, Lobaplatin, Miriplatin, Nedaplatin, Oxaliplatin, Picoplatin, or Satraplatin.
  • mTOR inhibitors as used herein is used for purposes of a material to inhibit the mTOR signaling pathway of the conventional anticancer agents or immunosuppressive agents.
  • the mTOR inhibitor may be rapamycin, temsirolimus, everolimus, ridaforolimus, MLN4924, XL388, GDC-0349, AZD2014, AZD8055, GSK105965, MLN0128 Ridaforlimus and the like.
  • VEGF inhibitor as used herein is any substance that decreases signaling by the VEGF-VEGFR pathway.
  • VEGF inhibitors can be, to name just a few examples, small molecules, peptides, polypeptides, proteins, including more specifically antibodies, including anti-VEGF antibodies, anti-VEGFR antibodies, intrabodies, maxibodies, minibodies, diabodies, Fc fusion proteins such as peptibodies, receptibodies, soluble VEGF receptor proteins and fragments, and a variety of others.
  • Many VEGF inhibitors work by binding to VEGF or to a VEGF receptor. Others work more indirectly by binding to factors that bind to VEGF or to a VEGF receptor or to other components of the VEGF signaling pathway.
  • VEGF inhibitors act by altering regulatory posttranslational modifications that modulate VEGF pathway signaling.
  • VEGF inhibitors in accordance with the invention also may act through more indirect mechanisms. Whatever the mechanism involved, as used herein, a VEGF inhibitor decreases the effective activity of the VEGF signaling pathway in a given circumstance over what it would be in the same circumstance in the absence of the inhibitor.
  • Non-limiting examples of VEGF inhibitors include: (a) 4TBPPAPC or a closely related compound described in US2003/0125339 or U.S. Pat. No. 6,995,162 which is herein incorporated by reference in its entirety, particularly in parts disclosing 4TBPPAPC and closely related VEGF inhibitors; (b) AMG 706 or a closely related substituted alkylamine derivative described in US2003/0125339 or US2003/0225106 or U.S. Pat. No.
  • Nexavar® or a closely related substituted omega-carboxyaryl diphenyl urea or derivative thereof described in WO00/42012, WO00/41698, US2005/0038080A1, US2003/0125359A1, US2002/0165394A1, US2001/003447A1, US2001/0016659A1, and US2002/013774A1 which are herein incorporated by reference in their entirety, particularly in parts disclosing these VEGF inhibitors;
  • VEGF inhibitors are the following: (a) 4TBPPAPC, as described in US2003/0125339 or U.S. Pat. No. 6,995,162 which is herein incorporated by reference in its entirety, particularly in parts disclosing 4TBPPAPC; (b) AMG 706, as described in US2003/0125339 or U.S. Pat. No.
  • the VEGF inhibitor is pegaptanib. In one embodiment, the VEGF inhibitor is bevacizumab. In one embodiment, the VEGF inhibitor is ranibizumab. In one embodiment, the VEGF inhibitor is lapatinib. In one embodiment, the VEGF inhibitor is sorafenib. In one embodiment, the VEGF inhibitor is sunitinib. In one embodiment, the VEGF inhibitor is axitinib. In one embodiment, the VEGF inhibitor is pazopanib. In one embodiment, the VEGF inhibitor is aflibercept. In one embodiment, the VEGF inhibitor is Ramucirumab.
  • aromatase inhibitor non-steroidal and steroidal compounds that inhibit the enzyme aromatase thereby preventing the conversion of androgens to estrogens, preferably those which inhibit aromatase activity in vitro with an IC50 value of less than 10 ⁇ 5 M as well as their pharmaceutically acceptable salts.
  • aromatase inhibitors for use in the methods herein described include without limitation anastrozole, letrozole, exemestane, vorozole, formestane, fadrozole, aminoglutethimide, testolactone, 4-hydroxyandrostenedione, 1,4,6-androstatrien-3,17-dione and 4-androstene-3,6,17-trione.
  • cyclin dependent kinase 4/6 inhibitor and “CDK4/6 inhibitor” as used herein refer to a compound that selectively targets, decreases, or inhibits at least one activity of CDK4 and/or CDK6.
  • Non-limiting examples of inhibitors of CDK4/6 include Abemaciclib (LY2835219), palbociclib (PD0332991), LEE-011 (ribociclib), LY2835219 (abemaciclib), G1T28-1, SHR6390, or P276-00, or a derivative of any one of palbociclib, LEE-011, G1T28-1, SHR6390, or P276-00.
  • the CDK4/6 inhibitor may be derived from pyridopyrimidine, pyrrolopyrimidine or indolocarbazole compounds.
  • a “molecularly targeted agent” is a substance that interferes with the function of a single molecule or group of molecules, preferably those that are involved in tumor growth and progression, when administered to a subject.
  • Non-limiting examples of molecularly targeted agent of this patent document include signal transduction inhibitors, modulators of gene expression and other cellular functions, immune system modulators, antibody-drug conjugates (ADCs), and combinations thereof.
  • the molecularly targeted agent may be selected from epidermal growth factor receptor family inhibitors (EGFRi), mammalian target of rapamycin (mTor) inhibitors, immune checkpoint inhibitors, anaplastic lymphoma kinase (ALK) inhibitors, B-cell lymphoma-2 (BCL-2) inhibitors, B-Raf inhibitors, cyclin-dependent kinase inhibitors (CDKi), ERK inhibitors, histone deacetylase inhibitors (HDACi), heat shock protein-90 inhibitors (HSP90i), Janus kinase inhibitors, mitogen activated protein kinase (MAPK) inhibitors, MEK inhibitors, poly ADP ribose polymerase (PARP) inhibitors, phosphoinositide 3-kinase inhibitors (PI3Ki), Ras inhibitors, and combinations thereof.
  • EGFRi epidermal growth factor receptor family inhibitors
  • MTK mammalian target of rapamycin
  • ALK ana
  • the molecularly targeted agent may be selected from ado-trastuzumab emtansine, alemtuzumab, cetuximab, ipilimumab, ofatumumab, panitumumab, pertuzumab, rituximab, tositumomab, 131I-tositumomab, trastuzumab, brentuximab vedotin, denileukin diftitox, ibritumomab tiuxetan, axitinib, bortezomib, bosutinib, cabozantinib, crizotinib, carfilzomib, dasatinib, erlotinib, gefitinib, imatinib mesylate, lapatinib, nilotinib, pazopanib, ponatin
  • the EGFRi may be selected from erlotinib, gefitinib, lapatinib, canetinib, pelitinib, neratinib, (R,E)-N-(7-chloro-1-(1-(4-(dimethylamino)but-2-enoyl)azepan-3-yl)-1H-benzo[d]imidazol-2-yl)-2-methylisonicotinamide, Trastuzumab, T-DM1, Margetuximab, panitumumab, matuzumab, Necitumumab, pertuzumab, nimotuzumab, zalutumumab, Necitumumab, cetuximab, icotinib, afatinib, and pharmaceutically acceptable salt thereof.
  • the molecularly targeted agent may be an anti-EGFR family antibody or a complex including the anti-EGFR family antibody.
  • Another aspect of the invention provides a method of treating a neoplasm in a subject comprising administering to a subject in need thereof the novel crystalline form described herein, the pharmaceutical composition thereof, a combination with one or more agents, or a kit containing the novel crystalline form.
  • Non-limiting examples of the neoplasm that can be treated with this patent document include lung cancer including non-small cell lung cancer, breast cancer, stomach cancer, colon cancer, pancreatic cancer, prostate cancer, myeloma, head and neck cancer, ovarian cancer, esophageal cancer, or metastatic cell carcinoma.
  • the neoplasm associated with overexpression or amplification of at least one gene of HER1, HER2, and HER4 or a mutant thereof may be an abnormal growth of tissue, which if it forms a mass, is commonly referred to as a tumor having overexpression of at least one of HER1, HER2, HER4 and mutant thereof or amplification of at least one gene coding of HER1, HER2, HER4 or mutant thereof.
  • the mutant may be HER1 having exon 19 deletion, T790M substitution, L828R substitution, or combination thereof.
  • overexpression indicates that the protein is expressed at a higher level than normal cells. The expression level can be measured using immunohistochemistry, fluorescence in situ hybridization (FISH), or chromogenic in situ hybridization (CISH).
  • wild type refers to a polypeptide or polynucleotide sequence that occurs in a native population without genetic modification.
  • a “mutant” includes a polypeptide or polynucleotide sequence having at least one modification to an amino acid or nucleic acid compared to the corresponding amino acid or nucleic acid found in a wild type polypeptide or polynucleotide, respectively. Included in the term mutant is Single Nucleotide Polymorphism (SNP) where a single base pair distinction exists in the sequence of a nucleic acid strand compared to the most prevalently found (wild type) nucleic acid strand.
  • SNP Single Nucleotide Polymorphism
  • Neoplasm including cancers that are either wild type or mutant for HER1, HER2, or HER4 or have amplification of HER1, HER2, or HER4 genes or have over expression of HER1, HER2, or HER4 protein are identified by known methods.
  • wild type or mutant HER1, HER2, and HER4 tumor cells can be identified by DNA amplification and sequencing techniques, DNA and RNA detection techniques, including, but not limited to Northern and Southern blot, respectively, and/or various biochip and array technologies or in-situ hybridization. Wild type and mutant polypeptides can be detected by a variety of techniques including, but not limited to immunodiagnostic techniques such as ELISA, Western blot or immunocytochemistry.
  • the cancer to be treated may be associated with EGFR and/or HER2 exon 20 mutations, such as exon 20 insertion mutations.
  • HER2 exon 20 mutations such as exon 20 insertion mutations.
  • the novel crystalline form of the compound of this patent document or its combination with one or more agents can be used for the treatment of NSCLC patients with EGFR exon 20 mutations.
  • the cancer to be treated with the novel crystalline form of the compound of this patent document or its combination with one or more agents is oral cancer, oropharyngeal cancer, nasopharyngeal cancer, respiratory cancer, urogenital cancer, gastrointestinal cancer, central or peripheral nervous system tissue cancer, an endocrine or neuroendocrine cancer or hematopoietic cancer, glioma, sarcoma, carcinoma, lymphoma, melanoma, fibroma, meningioma, brain cancer, oropharyngeal cancer, nasopharyngeal cancer, renal cancer, biliary cancer, pheochromocytoma, pancreatic islet cell cancer, Li-Fraumeni tumors, thyroid cancer, parathyroid cancer, pituitary tumors, adrenal gland tumors, osteogenic sarcoma tumors, multiple neuroendocrine type I and type II tumors, breast cancer, lung cancer, head and neck cancer, prostate cancer, esophageal cancer,
  • treating means: (1) to ameliorate or prevent the condition of one or more of the biological manifestations of the condition, (2) to interfere with (a) one or more points in the biological cascade that leads to or is responsible for the condition or (b) one or more of the biological manifestations of the condition, (3) to alleviate one or more of the symptoms, effects or side effects associated with the condition or treatment thereof, or (4) to slow the progression of the condition or one or more of the biological manifestations of the condition.
  • Prophylactic therapy is also contemplated thereby.
  • prevention is not an absolute term.
  • prevention is understood to refer to the prophylactic administration of a drug to substantially diminish the likelihood or severity of a condition or biological manifestation thereof, or to delay the onset of such condition or biological manifestation thereof.
  • Prophylactic therapy is appropriate, for example, when a subject is considered at high risk for developing cancer, such as when a subject has a strong family history of cancer or when a subject has been exposed to a carcinogen.
  • kits can further comprise a package insert.
  • the insert may contain, for example, instructions for treating a neoplasm associated with overexpression or amplification of certain genes or mutations, including for example, HER1, HER2, or HER4 or a mutant thereof, in a subject.
  • the kit or combination of the novel crystalline form of the compound of Formula (1) of this patent document may include poziotinib and an anti-EGFR family antibody.
  • the anti-EGFR family antibody may be trastuzumab, cetuximab, margetuximab, matuzumab, panitumumab, necitumumab, or pertuzumab.
  • An example of the combination may be poziotinib and trastuzumab; or poziotinib and cetuximab.
  • Poziotinib may be hydrochloride.
  • the combination may further include a cytotoxic agent.
  • the cytotoxic agent may be a mitotic inhibitor.
  • the mitotic inhibitor may be taxane, vinca alkaloid, epothilone, or a combination thereof.
  • the vinca alkaloid may be at least one drug selected from the group consisting of vinblastine, vincristine, vindesine, vincaminol, vinburnine,ieridine and vinorelbine.
  • An example of the combination may include poziotinib; and trastuzumab and vinorelbine.
  • the vinorelbine may be in the form of an injection.
  • the taxane may be paclitaxel or docetaxel.
  • An example of the combination may include poziotinib; and cetuximab and pacliataxel.
  • the paclitaxel may be in the form of an injection.
  • the novel crystalline form of the compound of Formula (1) of this patent document may be administered in an amount of 0.1 mg to 50 mg.
  • Trastuzumab may be administered in an amount of 0.5 mg to 10 mg per kg of a body weight.
  • Cetuximab may be administered in an amount of from 100 mg/m2 to 500 mg/m2 of a surface area of the body.
  • Trastuzumab sold under the brand name HerceptinTM among others, is a monoclonal antibody used to treat breast cancer. Specifically it is used for breast cancer that is HER2 receptor positive. Trastuzumab is given by slow injection into a vein and injection just under the skin.
  • Cetuximab is an epidermal growth factor receptor (EGFR) inhibitor used for the treatment of metastatic colorectal cancer, metastatic non-small cell lung cancer and head and neck cancer.
  • Cetuximab is a chimeric (mouse/human) monoclonal antibody given by intravenous infusion that is distributed under the trade name Erbitux in the U.S. and Canada by the drug company Bristol-Myers Squibb and outside the U.S. and Canada by the drug company Merck KGaA. In Japan, Merck KGaA, Bristol-Myers Squibb and Eli Lilly have a co-distribution.
  • Paclitaxel sold under the brand name Taxol among others, is a chemotherapy medication used to treat a number of types of cancer. This includes ovarian cancer, breast cancer, lung cancer, Kaposi sarcoma, cervical cancer, and pancreatic cancer. It is given by injection into a vein.
  • the kit/combination may include the novel crystalline form of the compound of Formula (1) of this patent document and a mitotic inhibitor.
  • the mitotic inhibitor may be selected from BT-062, HMN-214, eribulin mesylate, vindesine, EC-1069, EC-1456, EC-531, vintafolide, 2-methoxyestradiol, GTx-230, trastuzumab emtansine, crolibulin, D1302A-maytansinoid conjugates, IMGN-529, lorvotuzumab mertansine, SAR-3419, SAR-566658, IMP-03138, topotecan/vincristine combinations, BPH-8, fosbretabulin tromethamine, estramustine phosphate sodium, vincristine, vinflunine, vinorelbine, RX-21101, cabazitaxel, STA-9584, vinblastine, epothilone
  • Poziotinib may be administered in an amount of 0.1 mg to 50 mg.
  • vinorelbine may be administered in an amount of 0.5 mg/m2 to 50 mg/m2 of a surface area of the body.
  • paclitaxel may be administered in an amount of from 100 mg/m2 to 300 mg/m2 of a surface area of the body.
  • Vinorelbine sold under the brand name Navelbine among others, is a chemotherapy medication used to treat a number of types of cancer. This includes breast cancer and non-small cell lung cancer. It is given by injection into a vein or by mouth. Vinorelbine is in the vinca alkaloid family of medications. It is believed to work by disrupting the normal function of microtubules and thereby stopping cell division.
  • the combination may include the novel crystalline form of the compound of Formula (1) of this patent document and an mTOR inhibitor.
  • the mTOR inhibitor may be selected from zotarolimus, umirolimus, temsirolimus, sirolimus, sirolimus NanoCrystal, sirolimus TransDerm, sirolimus-PNP, everolimus, biolimus A9, ridaforolimus, rapamycin, TCD-10023, DE-109, MS-R001, MS-R002, MS-R003, Perceiva, XL-765, quinacrine, PKI-587, PF-04691502, GDC-0980, dactolisib, CC-223, PWT-33597, P-7170, LY-3023414, INK-128, GDC-0084, DS-7423, DS-3078, CC-115, CBLC-137, AZD-2014, X-480, X-414, EC-0371, VS-
  • novel crystalline form of the compound of Formula (1) of this patent document may be administered in an amount of 0.1 mg to 50 mg. Also, rapamycin may be administered in an amount of 0.5 mg/m2 to 10 mg/m2 of a surface area of the body.
  • the kit/combination may include the novel crystalline form of the compound of Formula (1) of this patent document and a platinum-based antineoplastic drug.
  • the platinum-based antineoplastic drug may be selected from the group consisting of cisplatin, carboplatin, dicycloplatin, eptaplatin, lobaplatin, miriplatin, nedaplatin, oxaliplatin, picoplatin, and satraplatin.
  • An example of the combination may include poziotinib and cisplatin.
  • the cisplatin may be in the form of an injection.
  • Cisplatin is a chemotherapy medication used to treat a number of cancers. This includes testicular cancer, ovarian cancer, cervical cancer, breast cancer, bladder cancer, head and neck cancer, esophageal cancer, lung cancer, mesothelioma, brain tumors and neuroblastoma. It is used by injection into a vein. Cisplatin is in the platinum-based antineoplastic family of medications. It works in part by binding to, and inhibiting DNA replication.
  • this patent document also provides for a method of treating a human patient at risk of developing a condition associated with overexpression or amplification of HER1, HER2, or HER4 or a mutant of HER1, HER2, or HER4 by first determining the predisposition of the such human patient individual to HER1, HER2 or HER4 mutation and then administering therapeutically effective combinations of agents in a kit as described herein.
  • compositions or agents in a kit may be presented in unit dose forms containing a predetermined amount of active ingredient per unit dose.
  • the amount of active ingredient per dose will depend on the condition being treated, the route of administration and the age, weight and condition of the patient.
  • Preferred unit dosage formulations are those containing a daily dose or sub-dose, or an appropriate fraction thereof, of an active ingredient.
  • such pharmaceutical formulations may be prepared by any of the methods well known in the pharmacy art.
  • Solid or liquid pharmaceutical carriers are employed.
  • Solid carriers include, starch, lactose, calcium sulfate dihydrate, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, and stearic acid.
  • Liquid carriers include syrup, peanut oil, olive oil, saline, and water.
  • the carrier may include a prolonged release material, such as glyceryl monostearate or glyceryl distearate, alone or with a wax.
  • the amount of solid carrier varies widely but, suitably, may be from about 25 mg to about 1 g per dosage unit.
  • the preparation will suitably be in the form of a syrup, elixir, emulsion, soft gelatin capsule, sterile injectable liquid such as an ampoule, or an aqueous or nonaqueous liquid suspension.
  • the active drug component can be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like.
  • an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like.
  • Powders are prepared by comminuting the compound to a suitable fine size and mixing with a similarly comminuted pharmaceutical carrier such as an edible carbohydrate, as, for example, starch or mannitol. Flavoring, preservative, dispersing and coloring agent can also be present.
  • formulations may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavoring agents.
  • X-ray Powder Diffraction (XRPD) spectroscopy was conducted on the D8 Advance (Bruker ASX, Germany) analyzer from 3°2 ⁇ to 40°2 ⁇ for the sample.
  • XRPD X-ray Powder Diffraction
  • DSC Differential Scanning Calorimeter
  • the solid state nuclear magnetic resonance (ssNMR) analysis was conducted at room temperature using a Bruker Avance II 500 MHz Solid NMR system (Bruker, Germany) analyzer after placing 100 mg of sample in the 4 mm sample tube.
  • the analysis conditions of the 13 C NMR spectra ( 13 C CP/MAS TOSS ssNMR) are as follows.
  • Ion Chromatograph (IC) analysis was conducted on the Thermo Fisher Scientific ICS-2500 series IC Systems (Thermo Fisher Scientific, USA) analyzer for the purpose of content analysis of hydrochloric acid in the hydrochloride salt.
  • the analysis conditions of the IC are as follows.
  • Moisture measurement was conducted using the 795KFT Titrino (Metrohm, Switzerland) Karl-Fischer moisture analyzer.
  • the compound of Reference Example showed a broad peak in the spectrum of ssNMR, which is a typical peak pattern of amorphous structure.
  • the compound of Reference Example did not exhibit any specific endotherm exotherm curve, as measured by the DSC (10° C./min).
  • the compound of Reference Example exhibited a tendency to continuously absorb moisture of 1-3% at a relative humidity range of 10-90%, as measured by the DVS.
  • the moisture content of the amorphous form was 2.1%, as measured by the Karl-Fischer moisture analyzer, and no characteristic melting point was observed.
  • FIGS. 1A, 2A, 3A and 4A The analysis results of XRPD, ssNMR, DSC and DVS for the crystalline form prepared in Example 1 are shown in FIGS. 1A, 2A, 3A and 4A , respectively.
  • Peaks with a relative intensity (I/I o ) of 15% or more of the crystalline form in the XRPD spectrum are summarized in Table 1 below.
  • peaks at the diffraction angle (2 ⁇ 0.2°) of 9.4, 11.4, 13.0, 16.1, 18.5, 19.3, 24.9 and 26.3° appeared.
  • Peaks of the chemical shift (ppm ⁇ 0.5 ppm) in the 13 C CP/MAS TOSS ssNMR spectrum of the crystalline form are summarized in Table 2 below.
  • the above crystalline form showed an endothermic peak of the lowest point at about 100.7° C. when running from a starting point of about 79° C. as measured by the DSC (10° C./min) and an endothermic peak of the lowest point at about 190.8° C. when running from a starting point of about 186.5° C.
  • the endothermic peak at about 100.7° C., as measured by the DSC means the dehydration point of the crystalline hydrate form of crystalline Form I of the compound of chemical formula (1), and the endothermic peak at about 190.8° C. means the melting point.
  • the crystalline form exhibited a moisture content (theoretical moisture content of 6.83%) of about 7.5% in the Karl-Fischer moisture analyzer and showed a condensation temperature of about 117-122° C. and a melting point of about 190-195° C.
  • Peaks of the chemical shift (ppm ⁇ 0.5 ppm) in the 13 C CP/MAS TOSS solid state nuclear magnetic resonance (ssNMR) spectrum of the crystalline form are summarized in Table 4 below.
  • the above crystalline form exhibited an endothermic peak of the lowest point at about 190.8° C. when running from a starting point of about 185.8° C., as measured by the DSC (10° C./min), and the endothermic peak at about 190.8° C. means the melting point.
  • the hygroscopic degree of the crystalline form was a level of about 0.3-0.5% in the relative humidity range of 10%-50%, as measured by the DVS, which is very low, and the hygroscopic degree in the range of 50-90% was measured to be a level of about 3%.
  • Peaks with a relative intensity (I/I o ) of 15% or more of the crystalline form in the XRPD spectrum are summarized in Table 5 below. In the case of a peak of I/I o ⁇ 30% or more, peaks at the diffraction angle (2 ⁇ 0.2°) of 4.9, 5.9, 11.8, 18.8 and 19.9° appeared.
  • Peaks of the chemical shift (ppm ⁇ 0.5 ppm) in the 13 C CP/MAS TOSS solid state nuclear magnetic resonance (ssNMR) spectrum of the crystalline form are summarized in Table 6 below.
  • the above crystalline form exhibited an endothermic peak of the lowest point at about 184.8° C. when running from a starting point of about 181.3° C. as measured by the DSC (10° C./min), and the endothermic peak at about 184.8° C. means the melting point.
  • the crystalline form exhibited a moisture content of about 0.3%, as measured by the Karl-Fischer moisture analyzer and showed a melting point of about 183-185° C.
  • the hygroscopic degree of the crystalline form was a level of about 0.7% in the relative humidity range of 10%-90%, as measured by the DVS, which is very low.
  • the crystalline form was sufficiently stable at the long-term storage conditions (e.g., 25° C. and relative humidity of 60%), accelerated conditions (e.g., 40° C. and relative humidity of 75%), and harsh conditions (e.g., 60° C.).
  • Peaks of the chemical shift (ppm ⁇ 0.5 ppm) in the 13 C CP/MAS TOSS solid state nuclear magnetic resonance (ssNMR) spectrum of the crystalline form are summarized in Table 8 below.
  • the crystalline form exhibited a moisture content of about 3.2% as measured by the Karl-Fischer moisture analyzer and showed a melting point of about 187-193° C.
  • the hygroscopic degree of the crystalline form was a level of about 0.4% in the relative humidity range of 10%-90%, as measured by the DVS, which is very low. It can be expected that the crystalline form absorbs moisture in long-term storage conditions (e.g., temperature of 25° C. and relative humidity of 60%) and accelerated conditions (e.g., temperature of 40° C. and relative humidity of 75%) to maintain the crystalline form of monohydrate.
  • long-term storage conditions e.g., temperature of 25° C. and relative humidity of 60%
  • accelerated conditions e.g., temperature of 40° C. and relative humidity of 75%
  • Peaks with a relative intensity (I/I o ) of 15% or more of the crystalline form in the XRPD spectrum are summarized in Table 9 below.
  • peaks at the diffraction angle (2 ⁇ 0.2°) of 9.5, 12.3, 13.0, 13.5, 14.2, 21.4, 23.0, 23.2, 23.5, 27.2 and 27.5° appeared.
  • Peaks of the chemical shift (ppm ⁇ 0.5 ppm) in the 13 C CP/MAS TOSS solid state nuclear magnetic resonance (ssNMR) spectrum of the crystalline form are summarized in Table 10 below.
  • the above crystalline form exhibited an endothermic peak of the lowest point at about 230.1° C. when running from a starting point of about 200.7° C., as measured by the DSC (10° C./min).
  • the endothermic peak at about 230.1° C. means the melting point.
  • the crystalline form exhibited a moisture content of about 0.1% in the Karl-Fischer moisture analyzer and showed a melting point of about 238-243° C.
  • the hygroscopic degree of the crystalline form was at a level of about 0.35% in the relative humidity range of 10%-90%, as measured by the DVS, which is very low.
  • the crystalline form did not absorb moisture in long-term storage conditions (e.g., temperature of 25° C. and relative humidity of 60%) and accelerated conditions (e.g., temperature of 40° C. and relative humidity of 75%) to maintain the crystalline form anhydrous.
  • the amorphous form did not show any diffraction value in the XRPD spectrum.
  • the amorphous form exhibited a very high hygroscopic degree at the relative humidity range of 10-90%, as measured by the DVS. Through this, it is expected to be unstable by absorbing moisture under long-term storage conditions (25° C. temperature and relative humidity of 60%) and accelerated conditions (40° C. temperature and relative humidity of 75%). Actually, a moisture absorption of 7-9% was confirmed under the 25° C., 60% relative humidity condition and the 40° C., 75% relative humidity condition.
  • the moisture content of the amorphous form was 1.2%, as measured by the Karl-Fischer moisture analyzer, and no characteristic melting point was observed.
  • Test Example 1 Comparative Test of Solubility of Amorphous Form and Crystalline Polymorphs of the Hydrochloride Salt
  • each of the polymorphs and amorphous form of the hydrochloride salt of the compound of chemical formula (1) prepared in Examples 4 to 6 was used to prepare samples under the following conditions according to non-ionized water and acidity (pH). Thereafter, each solution was analyzed by high performance liquid chromatography (HPLC) according to the measurement conditions of the content of the compound of chemical formula (1) to measure the dissolved amount (LOD: 0.1 ⁇ g/mL) based on the compound of chemical formula (1). The results calculated from the measured values are shown in Table 11 below.
  • the solubility of the hydrochloride salt of the compound of chemical formula (1) was remarkably higher than that of the compound of chemical formula (1) (less than 1.0 ⁇ g/mL), and the crystalline anhydrous form of the hydrochloride salt among crystalline polymorphs showed the highest solubility in water.
  • Test Example 2 Comparative Test of Stability of Amorphous Form and Crystalline Polymorphs of the Hydrochloride Salt
  • each of samples of the polymorphs and amorphous form of the hydrochloride salt of the compound of chemical formula (1) prepared in Examples 4 to 6 was left for 4 weeks under long term conditions (e.g., temperature of 25 ⁇ 2° C. and relative humidity of 60 ⁇ 5%) and accelerated conditions (e.g., temperature of 40° C. and relative humidity of 75%).
  • Each sample was analyzed by high performance liquid chromatography (HPLC) according to the purity measurement conditions of the compound of chemical formula (1). The purity measurement values (%) are shown in Table 12 below.
  • the crystalline hydrochloride salt form of the compound of chemical formula (1) was stable compared to the amorphous hydrochloride salt form of the compound of chemical formula (1), and in particular, the crystalline anhydrous form of the hydrochloride salt of the compound of chemical formula (1) showed the best results.
  • the crystalline anhydrous hydrochloride salt form of the compound of chemical formula (1) is expected to be the most advantageous in terms of the pharmaceutical composition when considering various physicochemical properties such as solubility, purity, stability, hygroscopicity, and melting point, etc.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Inorganic Chemistry (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
US17/275,290 2018-09-14 2019-09-12 Kits and methods for treating cancers Abandoned US20220040180A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US17/275,290 US20220040180A1 (en) 2018-09-14 2019-09-12 Kits and methods for treating cancers

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862731500P 2018-09-14 2018-09-14
US17/275,290 US20220040180A1 (en) 2018-09-14 2019-09-12 Kits and methods for treating cancers
PCT/US2019/050843 WO2020056156A1 (en) 2018-09-14 2019-09-12 Kits and methods for treating cancers

Publications (1)

Publication Number Publication Date
US20220040180A1 true US20220040180A1 (en) 2022-02-10

Family

ID=69776732

Family Applications (2)

Application Number Title Priority Date Filing Date
US17/275,382 Active 2039-11-12 US11883402B2 (en) 2018-09-14 2019-09-12 Crystalline forms of a quinazoline compound and its hydrochloride salts
US17/275,290 Abandoned US20220040180A1 (en) 2018-09-14 2019-09-12 Kits and methods for treating cancers

Family Applications Before (1)

Application Number Title Priority Date Filing Date
US17/275,382 Active 2039-11-12 US11883402B2 (en) 2018-09-14 2019-09-12 Crystalline forms of a quinazoline compound and its hydrochloride salts

Country Status (16)

Country Link
US (2) US11883402B2 (zh)
EP (2) EP3849964A4 (zh)
JP (2) JP2022500456A (zh)
KR (2) KR20210046818A (zh)
CN (2) CN112770758A (zh)
AR (2) AR116406A1 (zh)
AU (2) AU2019337640A1 (zh)
BR (2) BR112021004546A2 (zh)
CA (2) CA3110369A1 (zh)
IL (2) IL281167A (zh)
MX (2) MX2021002876A (zh)
PH (2) PH12021550432A1 (zh)
SG (2) SG11202102006YA (zh)
TW (2) TW202023562A (zh)
UY (2) UY38374A (zh)
WO (2) WO2020053816A1 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11883402B2 (en) 2018-09-14 2024-01-30 Hanmi Pharmaceutical Co., Ltd. Crystalline forms of a quinazoline compound and its hydrochloride salts
CN114642728A (zh) * 2021-06-02 2022-06-21 德琪(浙江)医药科技有限公司 mTOR抑制剂的制药用途及其药物组合物

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1596869B1 (en) * 2003-01-21 2014-06-04 New Form Pharmaceuticals Inc. Novel cocrystallization
KR100735639B1 (ko) * 2004-12-29 2007-07-04 한미약품 주식회사 암세포 성장 억제 효과를 갖는 퀴나졸린 유도체 및 이의제조방법
MX2007010399A (es) * 2005-02-26 2007-09-25 Astrazeneca Ab Derivados de quinazolina en la forma de inhibidores de cinasa de tirosina.
AU2007266535A1 (en) * 2006-05-31 2007-12-06 Abbott Laboratories Thiazole compounds as cannabinoid receptor ligands and uses thereof
WO2008012105A1 (en) 2006-07-28 2008-01-31 Synthon B.V. Crystalline erlotinib
EP2170844B1 (en) * 2007-02-21 2016-05-04 Natco Pharma Limited Novel polymorphs of erlotinib hydrochloride and method of preparation
TWI377944B (en) * 2007-06-05 2012-12-01 Hanmi Holdings Co Ltd Novel amide derivative for inhibiting the growth of cancer cells
KR101217526B1 (ko) * 2010-06-11 2013-01-02 한미사이언스 주식회사 아마이드 유도체 또는 이의 약학적으로 허용 가능한 염을 포함하는 약제학적 조성물
KR101317809B1 (ko) * 2011-06-07 2013-10-16 한미약품 주식회사 암세포의 성장을 억제하는 아마이드 유도체 및 비금속염 활택제를 포함하는 약학 조성물
KR101272613B1 (ko) * 2011-10-05 2013-06-10 한미사이언스 주식회사 1-(4-(4-(3,4-디클로로-2-플루오로페닐아미노)-7-메톡시퀴나졸린-6-일옥시)피페리딘-1-일)프로프-2-엔-1-온 염산염의 제조 방법 및 이에 사용되는 중간체
PE20152000A1 (es) * 2013-03-06 2016-01-22 Astrazeneca Ab Inhibidores quinazolinicos de formas mutadas activantes del receptor del factor de crecimiento epidermico
CN106068262B (zh) 2014-04-11 2019-10-29 海思科医药集团股份有限公司 喹唑啉衍生物及其制备方法和在医药上的应用
AU2016343517B2 (en) * 2015-10-19 2020-04-30 Sunshine Lake Pharma Co., Ltd. A salt of EGFR inhibitor, crystalline form and uses thereof
EP3528798A4 (en) * 2016-10-19 2020-10-21 United States Government as Represented by The Department of Veterans Affairs COMPOSITIONS AND METHODS OF TREATMENT OF CANCER
CA3044432A1 (en) 2016-11-17 2018-05-24 Board Of Regents, The University Of Texas System Compounds with anti-tumor activity against cancer cells bearing egfr or her2 exon 20 mutations
US11883402B2 (en) 2018-09-14 2024-01-30 Hanmi Pharmaceutical Co., Ltd. Crystalline forms of a quinazoline compound and its hydrochloride salts

Also Published As

Publication number Publication date
PH12021550456A1 (en) 2021-12-06
KR20210061371A (ko) 2021-05-27
CN112823153A (zh) 2021-05-18
PH12021550432A1 (en) 2021-11-29
SG11202101712XA (en) 2021-04-29
EP3849557A4 (en) 2022-06-08
JP2022500456A (ja) 2022-01-04
TW202027750A (zh) 2020-08-01
US20220056011A1 (en) 2022-02-24
WO2020056156A1 (en) 2020-03-19
AR116406A1 (es) 2021-05-05
EP3849964A1 (en) 2021-07-21
AU2019337640A1 (en) 2021-03-11
CA3111062A1 (en) 2020-03-19
EP3849964A4 (en) 2022-06-08
AR116669A1 (es) 2021-06-02
KR20210046818A (ko) 2021-04-28
EP3849557A1 (en) 2021-07-21
UY38375A (es) 2020-04-30
SG11202102006YA (en) 2021-04-29
MX2021002875A (es) 2021-07-15
AU2019340598A1 (en) 2021-03-18
BR112021004527A2 (pt) 2021-06-08
TW202023562A (zh) 2020-07-01
IL281168A (en) 2021-04-29
CN112770758A (zh) 2021-05-07
MX2021002876A (es) 2021-06-04
UY38374A (es) 2020-04-30
CA3110369A1 (en) 2020-03-19
WO2020053816A1 (en) 2020-03-19
BR112021004546A2 (pt) 2021-06-08
US11883402B2 (en) 2024-01-30
IL281167A (en) 2021-04-29
JP2022500416A (ja) 2022-01-04

Similar Documents

Publication Publication Date Title
US20220169638A1 (en) 6-pyrimidin-isoindole derivative as erk1/2 inhibitor
AU2022228084B2 (en) CRYSTALLINE FORMS OF C21H22Cl2N4O2
JP7291839B2 (ja) 固体形態のｃｄｋ４阻害薬
US11883402B2 (en) Crystalline forms of a quinazoline compound and its hydrochloride salts
KR20240005899A (ko) 암 치료를 위한 병용요법
EP3125901B1 (en) Derivatives of cephalosporin for treating cancer
US20180333415A1 (en) Therapeutic methods
JP7180977B2 (ja) 結晶性Ｃ２１Ｈ２２Ｃｌ２Ｎ４Ｏ２マロン酸塩
JP7289978B1 (ja) がん治療のための併用療法

Legal Events

Date Code Title Description
AS Assignment

Owner name: SPECTRUM PHARMACEUTICALS, CALIFORNIA

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:REDDY, GURU;REEL/FRAME:056331/0643

Effective date: 20210521

STPP Information on status: patent application and granting procedure in general

Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

AS Assignment

Owner name: SLR INVESTMENT CORP., AS COLLATERAL AGENT, NEW YORK

Free format text: SECURITY INTEREST;ASSIGNORS:SPECTRUM PHARMACEUTICALS, INC.;ALLOS THERAPEUTICS, INC.;TALON THERAPEUTICS, INC.;REEL/FRAME:061179/0202

Effective date: 20220921

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION

AS Assignment

Owner name: TALON THERAPEUTICS, INC., CALIFORNIA

Free format text: TERMINATION AND RELEASE OF INTELLECTUAL PROPERTY SECURITY AGREEMENT RECORDED AT REEL 061179/FRAME 0202;ASSIGNOR:SLR INVESTMENT CORP.;REEL/FRAME:064463/0709

Effective date: 20230731

Owner name: ALLOS THERAPEUTICS, INC., COLORADO

Free format text: TERMINATION AND RELEASE OF INTELLECTUAL PROPERTY SECURITY AGREEMENT RECORDED AT REEL 061179/FRAME 0202;ASSIGNOR:SLR INVESTMENT CORP.;REEL/FRAME:064463/0709

Effective date: 20230731

Owner name: SPECTRUM PHARMACEUTICALS, INC., MASSACHUSETTS

Free format text: TERMINATION AND RELEASE OF INTELLECTUAL PROPERTY SECURITY AGREEMENT RECORDED AT REEL 061179/FRAME 0202;ASSIGNOR:SLR INVESTMENT CORP.;REEL/FRAME:064463/0709

Effective date: 20230731