US20210317499A1 - Afucosylated antibodies and manufacture thereof - Google Patents

Afucosylated antibodies and manufacture thereof Download PDF

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Publication number
US20210317499A1
US20210317499A1 US17/270,987 US201817270987A US2021317499A1 US 20210317499 A1 US20210317499 A1 US 20210317499A1 US 201817270987 A US201817270987 A US 201817270987A US 2021317499 A1 US2021317499 A1 US 2021317499A1
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United States
Prior art keywords
antibody
afucosylated
cells
cell
modified enzyme
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Pending
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US17/270,987
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English (en)
Inventor
Wen-Jiun Peng
Hui-Jung Chen
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United Biopharma Inc
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United Biopharma Inc
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Assigned to United Biopharma Inc. reassignment United Biopharma Inc. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: CHEN, HUI-JUNG, PENG, WEN-JIUN
Publication of US20210317499A1 publication Critical patent/US20210317499A1/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01271GDP-L-fucose synthase (1.1.1.271)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01068Glycoprotein 6-alpha-L-fucosyltransferase (2.4.1.68), i.e. FUT8
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/01Hydro-lyases (4.2.1)
    • C12Y402/01047GDP-mannose 4,6-dehydratase (4.2.1.47), i.e. GMD
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/70Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/732Antibody-dependent cellular cytotoxicity [ADCC]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/70Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/734Complement-dependent cytotoxicity [CDC]
US17/270,987 2018-08-29 2018-08-29 Afucosylated antibodies and manufacture thereof Pending US20210317499A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2018/102995 WO2020042015A1 (en) 2018-08-29 2018-08-29 Afucosylated antibodies and manufacture thereof

Publications (1)

Publication Number Publication Date
US20210317499A1 true US20210317499A1 (en) 2021-10-14

Family

ID=69642839

Family Applications (1)

Application Number Title Priority Date Filing Date
US17/270,987 Pending US20210317499A1 (en) 2018-08-29 2018-08-29 Afucosylated antibodies and manufacture thereof

Country Status (11)

Country Link
US (1) US20210317499A1 (zh)
EP (1) EP3818149A4 (zh)
JP (1) JP7216806B2 (zh)
KR (1) KR102596303B1 (zh)
CN (1) CN113166728A (zh)
AU (1) AU2018438767B9 (zh)
BR (1) BR112021003765A2 (zh)
CA (1) CA3110254C (zh)
MY (1) MY197429A (zh)
SG (1) SG11202101099PA (zh)
WO (1) WO2020042015A1 (zh)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7292377B2 (ja) * 2018-08-29 2023-06-16 ユナイテッド バイオファーマ インコーポレイテッド アフコシル化抗体およびその製造法
AR126089A1 (es) 2021-06-07 2023-09-13 Amgen Inc Uso de fucosidasa para controlar el nivel de afucosilación de proteínas glucosiladas
CN113684322B (zh) * 2021-09-09 2024-01-23 上海药明生物技术有限公司 一种降低抗体类蛋白高聚甘露糖型水平的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2785941C (en) * 2000-10-06 2017-01-10 Kyowa Hakko Kirin Co., Ltd. Antibody composition-producing cell
JPWO2003085107A1 (ja) * 2002-04-09 2005-08-11 協和醗酵工業株式会社 ゲノムが改変された細胞
EP1888638A2 (en) * 2005-06-03 2008-02-20 Genentech, Inc. Method of producing antibodies with modified fucosylation level
WO2008090958A1 (ja) * 2007-01-24 2008-07-31 Kyowa Hakko Kirin Co., Ltd. ドメイン交換された遺伝子組換え抗体組成物
KR101441437B1 (ko) * 2009-06-02 2014-09-25 리제너론 파마슈티칼스 인코포레이티드 푸코실화-결핍 세포
US8469819B2 (en) * 2009-06-04 2013-06-25 Michael Parker McMain Game apparatus and game control method for controlling and representing magical ability and power of a player character in an action power control program
EP2543727B1 (en) * 2010-03-02 2016-08-31 Kyowa Hakko Kirin Co., Ltd. Modified antibody composition
ES2687771T3 (es) * 2011-03-06 2018-10-29 Merck Serono Sa Líneas de células con bajo nivel de fucosa y sus usos
IL217216A0 (en) * 2011-12-27 2012-07-31 Merck Serono Sa Low fucose cell lins and uses thereof
WO2017079165A1 (en) * 2015-11-02 2017-05-11 Genentech, Inc. Methods of making fucosylated and afucosylated forms of a protein
CN106167525B (zh) * 2016-04-01 2019-03-19 北京康明百奥新药研发有限公司 筛选超低岩藻糖细胞系的方法和应用
CN106701823A (zh) * 2017-01-18 2017-05-24 上海交通大学 生产无岩藻糖单克隆抗体的cho细胞系建立及其应用
CN107881160A (zh) * 2017-08-11 2018-04-06 百奥泰生物科技(广州)有限公司 一种由基因组被编辑的cho宿主细胞产生的具有独特糖谱的重组抗体及其制备方法

Also Published As

Publication number Publication date
AU2018438767B9 (en) 2023-10-05
KR102596303B1 (ko) 2023-10-30
EP3818149A4 (en) 2022-03-23
KR20210047316A (ko) 2021-04-29
CN113166728A (zh) 2021-07-23
JP7216806B2 (ja) 2023-02-01
JP2021534801A (ja) 2021-12-16
WO2020042015A1 (en) 2020-03-05
BR112021003765A2 (pt) 2021-05-25
AU2018438767A1 (en) 2021-04-01
MY197429A (en) 2023-06-16
SG11202101099PA (en) 2021-03-30
CA3110254A1 (en) 2020-03-05
CA3110254C (en) 2023-08-29
AU2018438767B1 (zh) 2023-07-13
EP3818149A1 (en) 2021-05-12

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