US20170251677A1 - CONCENTRATED EXTRACT OF ALGAE, PRODUCTION METHOD THEREOF AND USE OF SAME lN AGRICULTURE - Google Patents

CONCENTRATED EXTRACT OF ALGAE, PRODUCTION METHOD THEREOF AND USE OF SAME lN AGRICULTURE Download PDF

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US20170251677A1
US20170251677A1 US15/510,538 US201515510538A US2017251677A1 US 20170251677 A1 US20170251677 A1 US 20170251677A1 US 201515510538 A US201515510538 A US 201515510538A US 2017251677 A1 US2017251677 A1 US 2017251677A1
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extract
plant
seedling
roots
planting
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Paul Hery
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Laboratoires Goemar SA
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Laboratoires Goemar SA
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/03—Algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06—Coating or dressing seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

Definitions

  • the present invention relates to a concentrated algal extract having a percentage dry matter of between 6 and 100%, the preparation process thereof and also the uses thereof alone or combined with another nutritive, biostimulating or plant-protecting technology, especially to promote plant emergence, growth, development and reproduction and to prevent birds from eating the seeds.
  • the invention also targets a process for applying the concentrated algal extract of the present invention, and also a seed coated with said extract.
  • Yield largely depends on the good development of the plant and consequently on its growth, as well as its good health.
  • Plant growth is directly linked to the absorption and assimilation of mineral elements as well as the flows of hormones governing the elongation and differentiation of plant tissues. Consequently, nutrients and hormones play an essential role in plant growth. Owing to their inability to move, plants have developed active and activatable mechanisms enabling them to make the best possible use of the available resources to promote their growth. Fertilizers are used in order to increase this growth.
  • Algae have been used for a number of years as organic enriching agent on various soil types and various market garden and cereal crops. Algal extracts are used as biostimulant, frequently sprayed onto the leaves of the plants. Numerous beneficial effects linked to the use of algal extracts in agriculture have been observed and include, especially, an increase in the yield, in the nutrient supply, in resistance to frost and to stress conditions, an improved period of preservation of fruits and a reduction in attacks by fungi and insects.
  • Algal extracts are also known to enable better seed germination, especially by promoting plant emergence. Indeed, as soon as they emerge, seedlings are sensitive to pathogenic microorganisms such as fungal species such as rhizoctonia root rot, pythium, or bacteria which cause a disease known as damping off.
  • pathogenic microorganisms such as fungal species such as rhizoctonia root rot, pythium, or bacteria which cause a disease known as damping off.
  • the young shoots which are attacked end up lying on their side, as if the base of the stem has been pinched. Air humidity and low temperatures promote the appearance of damping off.
  • step c) optionally diafiltration or electrodialysis of the permeate obtained at the end of step c);
  • step e) optionally reducing, to the powder thereof, the permeate obtained at the end of step c) or the retentate obtained at the end of step d).
  • an algal extract in which the compounds of the intracellular fluid of the alga are preserved, had excellent biostimulating activity when it was concentrated to achieve a certain percentage dry matter.
  • the concentrated algal extract of the invention is more effective than a diluted extract in stimulating plant emergence and promoting plant growth.
  • the concentrated algal extract is used to coat seeds, the latter are less frequently attacked by birds than uncoated seeds.
  • a subject of the present invention is a concentrated algal extract having a percentage dry matter of between 6 and 100%, preferably between 9 and 70%, more preferentially between 12 and 50%, and containing 0 to 5%, in particular 1 to 4%, more particularly 2 to 3% by weight of alginate and/or of cellulose relative to the weight of said extract.
  • a second subject of the present invention is a process for preparing a concentrated algal extract according to the invention, comprising the following steps:
  • Another subject of the present invention is a process for promoting the emergence, growth, development and/or reproduction of a plant, characterized in that the extract according to the invention, or prepared according to the process of the invention, is applied by infiltration into the soil, sprayed onto the leaves of the plant, applied to a seed before sowing, or applied to the roots of a seedling, especially by dip treatment, before the planting thereof.
  • Yet another subject of the present invention is the use of an extract according to the invention, or prepared according to the process of the invention, to promote plant growth.
  • Another subject of the present invention is the use of an extract according to the invention, or prepared according to the process of the invention, to stimulate plant emergence.
  • a sixth subject of the present invention is the use of an extract according to the invention, or prepared according to the process of the invention, as bird repellent.
  • Another subject of the invention is a seed coated with an extract according to the invention, or prepared according to the process of the invention.
  • the concentrated algal extract of the present invention is especially an aqueous extract or a dry extract, having a percentage dry matter of between 6 and 100%, preferably between 9 and 70%, more preferentially between 12 and 50%.
  • m dry is the weight of matter remaining after drying; and m hydrated is the weight of the algal extract before drying.
  • the concentrated algal extract of the present invention does not contain alginate and/or cellulose.
  • the algal extract according to the invention comprises algal intracellular fluid, obtained after rupturing the algal cell walls and removing them by flocculation.
  • the concentrated algal extract of the present invention contains 0 to 5%, in particular 1 to 4%, more particularly 2 to 3% by weight of alginate and/or of cellulose relative to the weight of said extract.
  • the alga from which the concentrated extract of the present invention is obtained may especially be chosen from Ascophyllum nodosum, Fucus vesiculosus, Laminaria digitata, Laminaria hyperborea, Laminaria saccharina, Eklonia maxima, Sargassum spp. and mixtures thereof.
  • said alga is Ascophyllum nodosum.
  • the concentrated algal extract of the present invention may especially have a pH of between 1.5 and 9, preferably between 2 and 7, more preferentially between 2.5 and 3.
  • the concentrated algal extract of the present invention comprises algal intracellular fluid. It may especially comprise one or more compounds chosen from polysaccharides, such as, especially, laminarin and fucans; free and conjugated sugars; polyphenols; mannitol; growth hormones; lipids; proteins; amino acids; vitamins; betaines; sterols; glucuronic acid and mineral salts.
  • the concentrated algal extract of the present invention comprises laminarin; fucans; lipids; proteins and polyphenols.
  • the concentrated algal extract of the present invention may be formulated in a composition comprising said extract and at least one compound chosen from a gelling agent, a thickener, a plant-protection product, a second plant and/or algal extract, minerals, a surfactant, an oil, a preservative, and mixtures thereof.
  • the concentrated algal extract of the present invention may especially be obtained by the process described below.
  • the process for preparing a concentrated algal extract according to the present invention comprises the following steps:
  • the process of the present invention does not contain a step of hydrolysis, in the presence of a reducing agent, for example at a temperature of between 50 and 100° C., for a period of between 4 and 8 h.
  • a reducing agent for example at a temperature of between 50 and 100° C., for a period of between 4 and 8 h.
  • the grinding step especially makes it possible to rupture the algal cell walls, thereby releasing the algal intracellular fluid.
  • the grinding step is preferentially performed on fresh algae, that is to say algae which were harvested within the last 24 h.
  • the step of homogenizing the ground algal material may especially be carried out by adding water at an amount of from 1 to 2 l of water per kg of ground algal material.
  • the flocculation and filtration step especially makes it possible to remove the residues of the algal cell walls in order to recover the algal intracellular fluid.
  • the residues of the algal cell walls may especially precipitate by flocculation, by adding an acid, especially hydrochloric acid, to the ground algal material.
  • the acid is added so as to obtain a pH of between 1.5 and 4, preferably between 2 and 3.5, more preferentially between 2.5 and 3.
  • the acidified mixture is then filtered in order to remove the flocs of alginate and of cellulose which are larger than 40 ⁇ m.
  • the pH of the filtrate may optionally be adjusted by adding an acid or a base so as to reach a pH of between 1.5 and 9, preferably between 2 and 7, more preferentially between 2.5 and 3.
  • the step of concentrating the filtrate may be performed in various ways, such as especially reverse osmosis, water evaporation, algae enrichment or centrifugation.
  • Reverse osmosis is a membrane separation technique based on a process of selectivity by diffusion. Two algal extracts of different concentrations are placed on either side of a water-permeable membrane. When pressure is applied to the most concentrated algal extract, a portion of the water contained in the most concentrated extract migrates towards the least concentrated extract.
  • the water may be evaporated especially by heating the algal extract under reduced pressure, for example using a rotary evaporator.
  • the heating may be carried out between 40 and 90° C., preferably between 60 and 80° C.
  • the algae enrichment may especially be carried out by replacing, in the step of homogenizing the ground algal material of the process according to the invention, the water with an algal suspension obtained by the process according to the invention.
  • the centrifugation may especially be carried out by subjecting the algal extract to centrifugal force in a machine rotating at high speed.
  • the step of concentrating the filtrate is preferably carried out by water evaporation.
  • the concentrated algal extract according to the present invention is advantageously used in agriculture, where it has numerous applications depending on the type of plant to which it is applied and on the period of the plant cycle during which it is used.
  • the concentrated algal extract of the present invention may especially be used alone or combined with a nutritive, biostimulating or plant-protecting composition.
  • the plants on which the concentrated algal extract of the present invention may be used are agronomically useful plants, for example vegetable, fruit and cereal crops and ornamental plants.
  • the agronomically useful plants are angiosperms chosen from the group comprising Apiaceae, Asteraceae, Brassicaceae, Chenopodiaceae, Convolvulaceae, Cucurbitaceae, Fabaceae, Gramineae, Liliaceae, Polygonaceae, Rosaceae, Solanaceae, Poaceae and Vitaceae .
  • the concentrated algal extract of the present invention is used on tomatoes, rice, soya, fruit trees, vines, wheat or corn.
  • the concentrated algal extract of the present invention when used to promote growth of the plant, said extract may especially be applied by foliar spraying or by application to the soil or root infiltration.
  • the applications may be carried out throughout the growth of the plant, once or several times per week. Very beneficial results have been demonstrated on tomato seedlings and corn seedlings.
  • said extract may especially be applied to the seeds before they are planted, or to the young seedlings just after they emerge.
  • the seeds may especially be coated with the concentrated algal extract of the present invention.
  • the coating may for example be carried out by wetting the seeds with said extract and letting them dry, preferably at ambient temperature.
  • the application may be carried out at an amount of from 10 to 120 g, preferably 20 to 110 g, more preferentially 30 to 105 g of concentrated algal extract per 100 kg of seeds. Very beneficial results have been demonstrated on corn seeds.
  • the coated seed which is a subject of the present invention may especially be prepared as described in the previous paragraph. Coating the seed with the concentrated algal extract of the present invention enables quicker emergence of the plant and makes it more resistant to damping off, compared to uncoated seeds. Moreover, the coated seeds according to the present invention are much less frequently attacked by birds, such as especially crows, pigeons, skylarks, partridges and pheasants, compared to uncoated seeds.
  • FIG. 1 is a schematic representation of the number of corn seedlings obtained 3 weeks after sewing uncoated corn seeds, corn seeds coated with an algal extract of the present invention or corn seeds coated with a conventional fungicide as described in example 4.
  • FIG. 2 is a schematic representation of the number of corn seedlings obtained 3 weeks after sewing uncoated corn seeds, corn seeds coated with an algal extract of the present invention or corn seeds coated with a conventional fungicide as described in example 5.
  • An extract of Ascophyllum nodosum algae having a percentage dry matter of 5.1% is concentrated by water evaporation by heating the extract at 50° C. in a rotary evaporator under (water-pump) vacuum until the percentage dry matter reaches 13%.
  • the tomato seedlings are thus treated with comparable amounts of each extract. After 5 weeks of treatment, the tomato seedlings treated with the concentrated algal extract of the present invention (example 1) are taller and bushier than the seedlings treated with the non-concentrated algal extract (GOACTIV).
  • the concentrated algal extract of the present invention is therefore more effective at promoting the growth of tomato seedlings than a more diluted extract.
  • coated corn seeds prepared in example 3 are sown on 20 m 2 plots.
  • a control is carried out with untreated corn seeds.
  • a comparative test is carried out with corn seeds coated with a conventional fungicide comprising 25 g/l of fludioxonil and 9.7 g/l of metalaxyl-M.
  • the field was subjected to a particularly aggressive attack by crows.
  • the birds attack the seeds buried in the soil, which leads to a reduction in the number of healthy corn seedlings relative to the number of seeds planted.
  • the concentrated algal extract of the present invention therefore has a bird-repelling effect.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Forests & Forestry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Seaweed (AREA)
  • Catching Or Destruction (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cultivation Of Plants (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
US15/510,538 2014-09-11 2015-09-11 CONCENTRATED EXTRACT OF ALGAE, PRODUCTION METHOD THEREOF AND USE OF SAME lN AGRICULTURE Pending US20170251677A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR14/58561 2014-09-11
FR1458561A FR3025699B1 (fr) 2014-09-11 2014-09-11 Extrait d'algue concentre, procede de preparation et ses utilisations en agriculture
PCT/FR2015/052439 WO2016038320A1 (fr) 2014-09-11 2015-09-11 Extrait d'algues concentré, procédé de préparation et ses utilisations en agriculture

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US17/490,664 Division US20220015375A1 (en) 2014-09-11 2021-09-30 Concentrated extract of algae, production method thereof and use of same in agriculture
US17/490,234 Division US20220015374A1 (en) 2014-09-11 2021-09-30 Concentrated extract of algae, production method thereof and use of same in agriculture
US17/490,794 Division US20220015376A1 (en) 2014-09-11 2021-09-30 Concentrated extract of algae, production method thereof and use of same in agriculture

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US17/490,794 Pending US20220015376A1 (en) 2014-09-11 2021-09-30 Concentrated extract of algae, production method thereof and use of same in agriculture
US17/490,664 Pending US20220015375A1 (en) 2014-09-11 2021-09-30 Concentrated extract of algae, production method thereof and use of same in agriculture
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US17/490,234 Pending US20220015374A1 (en) 2014-09-11 2021-09-30 Concentrated extract of algae, production method thereof and use of same in agriculture

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US (4) US20170251677A1 (ru)
EP (1) EP3190895A1 (ru)
JP (2) JP6997621B2 (ru)
KR (1) KR102433919B1 (ru)
CN (2) CN113243394A (ru)
AR (1) AR101842A1 (ru)
AU (1) AU2015314041B2 (ru)
BR (1) BR112017004908A2 (ru)
CA (1) CA2960440A1 (ru)
CL (1) CL2017000562A1 (ru)
CO (1) CO2017002360A2 (ru)
DE (1) DE15775762T1 (ru)
DO (1) DOP2017000062A (ru)
EA (1) EA033192B1 (ru)
EC (1) ECSP17014987A (ru)
ES (1) ES2936296T1 (ru)
FR (1) FR3025699B1 (ru)
MA (1) MA40560A (ru)
MD (1) MD20170044A2 (ru)
MY (1) MY193956A (ru)
NI (1) NI201700028A (ru)
NZ (1) NZ729879A (ru)
PE (1) PE20171377A1 (ru)
PH (1) PH12017500446A1 (ru)
TN (1) TN2017000081A1 (ru)
UA (1) UA123896C2 (ru)
WO (1) WO2016038320A1 (ru)

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WO2021069866A1 (en) 2019-10-07 2021-04-15 UPL Corporation Limited Concentrated algal extract
WO2021140327A1 (en) 2020-01-08 2021-07-15 UPL Corporation Limited Seaweed extract composition for agriculture
CN114793530A (zh) * 2022-04-13 2022-07-29 江苏海洋大学 一种利用海藻提取液促进决明子种子萌发及生长的方法
WO2022269523A1 (en) * 2021-06-24 2022-12-29 Pi Industries Ltd. A combination of flubendiamide and seaweed extract

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FR3025699B1 (fr) * 2014-09-11 2017-11-03 Laboratoires Goemar Extrait d'algue concentre, procede de preparation et ses utilisations en agriculture
AU2019305154A1 (en) * 2018-07-18 2021-03-11 Valagro S.P.A. Composition and uses thereof in agriculture
CN109251086B (zh) * 2018-10-18 2021-12-10 中国农业大学 一种苜蓿抗旱型种衣剂及其应用
FR3097544B1 (fr) * 2019-06-18 2022-04-15 Algaia Composition et méthode pour améliorer la croissance de plantes
WO2021193971A1 (ja) * 2020-03-26 2021-09-30 節三 田中 植物の特性を増強する方法
JP6864304B1 (ja) * 2020-09-15 2021-04-28 節三 田中 植物の特性を増強する方法
CN111943774B (zh) * 2020-07-14 2023-03-21 湖北绿道农业发展有限公司 功能生物有机肥及其制备方法
CN112314632A (zh) * 2020-07-28 2021-02-05 珠海红塔仁恒包装股份有限公司 一种长效抑菌的抑菌剂及其白卡纸和制备方法
CN112471177A (zh) * 2020-11-26 2021-03-12 安徽金敦福农业科技有限公司 一种绿色农作物控旺产品制备方法及其使用方法

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Publication number Priority date Publication date Assignee Title
WO2021069866A1 (en) 2019-10-07 2021-04-15 UPL Corporation Limited Concentrated algal extract
CN114867352A (zh) * 2019-10-07 2022-08-05 Upl毛里求斯有限公司 浓缩藻类提取物
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CN114793530A (zh) * 2022-04-13 2022-07-29 江苏海洋大学 一种利用海藻提取液促进决明子种子萌发及生长的方法

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BR112017004908A2 (pt) 2018-04-10
DOP2017000062A (es) 2017-05-15
US20220015374A1 (en) 2022-01-20
AU2015314041A1 (en) 2017-03-30
CL2017000562A1 (es) 2017-10-06
US20220015376A1 (en) 2022-01-20
EA201790589A1 (ru) 2017-06-30
NI201700028A (es) 2017-05-02
PH12017500446B1 (en) 2017-07-31
PH12017500446A1 (en) 2017-07-31
CN113243394A (zh) 2021-08-13
KR20170058368A (ko) 2017-05-26
MY193956A (en) 2022-11-03
AU2015314041B2 (en) 2019-05-23
MD20170044A2 (ru) 2017-10-31
TN2017000081A1 (fr) 2018-07-04
EA033192B1 (ru) 2019-09-30
MA40560A (fr) 2016-03-17
JP2017533258A (ja) 2017-11-09
CN106714559A (zh) 2017-05-24
FR3025699A1 (fr) 2016-03-18
UA123896C2 (uk) 2021-06-23
JP6997621B2 (ja) 2022-01-17
NZ729879A (en) 2022-12-23
CA2960440A1 (fr) 2016-03-17
DE15775762T1 (de) 2023-05-04
US20220015375A1 (en) 2022-01-20
ES2936296T1 (es) 2023-03-15
ECSP17014987A (es) 2017-06-30
FR3025699B1 (fr) 2017-11-03
KR102433919B1 (ko) 2022-08-17
PE20171377A1 (es) 2017-09-15
JP2020099332A (ja) 2020-07-02
EP3190895A1 (fr) 2017-07-19
AR101842A1 (es) 2017-01-18
CO2017002360A2 (es) 2017-06-09

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