US20140161728A1 - Composition for detecting biofilms on viable tissues - Google Patents
Composition for detecting biofilms on viable tissues Download PDFInfo
- Publication number
- US20140161728A1 US20140161728A1 US13/990,755 US201113990755A US2014161728A1 US 20140161728 A1 US20140161728 A1 US 20140161728A1 US 201113990755 A US201113990755 A US 201113990755A US 2014161728 A1 US2014161728 A1 US 2014161728A1
- Authority
- US
- United States
- Prior art keywords
- composition
- biofilm
- staining
- derivatives
- biofilms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 71
- 238000010186 staining Methods 0.000 claims abstract description 58
- 239000003795 chemical substances by application Substances 0.000 claims description 46
- 238000001514 detection method Methods 0.000 claims description 17
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 14
- -1 phenanthraline Chemical compound 0.000 claims description 12
- 230000003287 optical effect Effects 0.000 claims description 8
- 239000004411 aluminium Substances 0.000 claims description 6
- 229910052782 aluminium Inorganic materials 0.000 claims description 6
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 6
- 239000004094 surface-active agent Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 5
- XJGFWWJLMVZSIG-UHFFFAOYSA-N 9-aminoacridine Chemical compound C1=CC=C2C(N)=C(C=CC=C3)C3=NC2=C1 XJGFWWJLMVZSIG-UHFFFAOYSA-N 0.000 claims description 4
- 241000195940 Bryophyta Species 0.000 claims description 4
- RZSYLLSAWYUBPE-UHFFFAOYSA-L Fast green FCF Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC(O)=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 RZSYLLSAWYUBPE-UHFFFAOYSA-L 0.000 claims description 4
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical compound C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 4
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 claims description 4
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 4
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 claims description 4
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical compound C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 claims description 4
- DZBUGLKDJFMEHC-UHFFFAOYSA-N acridine Chemical compound C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 claims description 4
- 229960001441 aminoacridine Drugs 0.000 claims description 4
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 claims description 4
- IINNWAYUJNWZRM-UHFFFAOYSA-L erythrosin B Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(I)C(=O)C(I)=C2OC2=C(I)C([O-])=C(I)C=C21 IINNWAYUJNWZRM-UHFFFAOYSA-L 0.000 claims description 4
- 229940011411 erythrosine Drugs 0.000 claims description 4
- 239000004174 erythrosine Substances 0.000 claims description 4
- 235000012732 erythrosine Nutrition 0.000 claims description 4
- 238000002189 fluorescence spectrum Methods 0.000 claims description 4
- 238000005187 foaming Methods 0.000 claims description 4
- 239000003906 humectant Substances 0.000 claims description 4
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 4
- 235000011929 mousse Nutrition 0.000 claims description 4
- BBEAQIROQSPTKN-UHFFFAOYSA-N pyrene Chemical compound C1=CC=C2C=CC3=CC=CC4=CC=C1C2=C43 BBEAQIROQSPTKN-UHFFFAOYSA-N 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 239000004088 foaming agent Substances 0.000 claims description 3
- 229940113601 irrigation solution Drugs 0.000 claims description 3
- TUFFYSFVSYUHPA-UHFFFAOYSA-M rhodamine 123 Chemical compound [Cl-].COC(=O)C1=CC=CC=C1C1=C(C=CC(N)=C2)C2=[O+]C2=C1C=CC(N)=C2 TUFFYSFVSYUHPA-UHFFFAOYSA-M 0.000 claims description 3
- 229940043267 rhodamine b Drugs 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- OYINILBBZAQBEV-UWJYYQICSA-N (17s,18s)-18-(2-carboxyethyl)-20-(carboxymethyl)-12-ethenyl-7-ethyl-3,8,13,17-tetramethyl-17,18,22,23-tetrahydroporphyrin-2-carboxylic acid Chemical compound N1C2=C(C)C(C=C)=C1C=C(N1)C(C)=C(CC)C1=CC(C(C)=C1C(O)=O)=NC1=C(CC(O)=O)C([C@@H](CCC(O)=O)[C@@H]1C)=NC1=C2 OYINILBBZAQBEV-UWJYYQICSA-N 0.000 claims description 2
- 125000004804 1-methylmethylene group Chemical group [H]C([H])([H])C([H])([*:2])[*:1] 0.000 claims description 2
- UTXPWBKKZFLMPZ-UHFFFAOYSA-N 2-aminoacridine Chemical compound C1=CC=CC2=CC3=CC(N)=CC=C3N=C21 UTXPWBKKZFLMPZ-UHFFFAOYSA-N 0.000 claims description 2
- MHIITNFQDPFSES-UHFFFAOYSA-N 25,26,27,28-tetrazahexacyclo[16.6.1.13,6.18,11.113,16.019,24]octacosa-1(25),2,4,6,8(27),9,11,13,15,17,19,21,23-tridecaene Chemical class N1C(C=C2C3=CC=CC=C3C(C=C3NC(=C4)C=C3)=N2)=CC=C1C=C1C=CC4=N1 MHIITNFQDPFSES-UHFFFAOYSA-N 0.000 claims description 2
- MGADZUXDNSDTHW-UHFFFAOYSA-N 2H-pyran Chemical compound C1OC=CC=C1 MGADZUXDNSDTHW-UHFFFAOYSA-N 0.000 claims description 2
- BCHZICNRHXRCHY-UHFFFAOYSA-N 2h-oxazine Chemical compound N1OC=CC=C1 BCHZICNRHXRCHY-UHFFFAOYSA-N 0.000 claims description 2
- KKAJSJJFBSOMGS-UHFFFAOYSA-N 3,6-diamino-10-methylacridinium chloride Chemical compound [Cl-].C1=C(N)C=C2[N+](C)=C(C=C(N)C=C3)C3=CC2=C1 KKAJSJJFBSOMGS-UHFFFAOYSA-N 0.000 claims description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims description 2
- KFKRXESVMDBTNQ-UHFFFAOYSA-N 3-[18-(2-carboxylatoethyl)-8,13-bis(1-hydroxyethyl)-3,7,12,17-tetramethyl-22,23-dihydroporphyrin-21,24-diium-2-yl]propanoate Chemical class N1C2=C(C)C(C(C)O)=C1C=C(N1)C(C)=C(C(O)C)C1=CC(C(C)=C1CCC(O)=O)=NC1=CC(C(CCC(O)=O)=C1C)=NC1=C2 KFKRXESVMDBTNQ-UHFFFAOYSA-N 0.000 claims description 2
- IPJDHSYCSQAODE-UHFFFAOYSA-N 5-chloromethylfluorescein diacetate Chemical compound O1C(=O)C2=CC(CCl)=CC=C2C21C1=CC=C(OC(C)=O)C=C1OC1=CC(OC(=O)C)=CC=C21 IPJDHSYCSQAODE-UHFFFAOYSA-N 0.000 claims description 2
- XYJODUBPWNZLML-UHFFFAOYSA-N 5-ethyl-6-phenyl-6h-phenanthridine-3,8-diamine Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2N(CC)C1C1=CC=CC=C1 XYJODUBPWNZLML-UHFFFAOYSA-N 0.000 claims description 2
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 claims description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 claims description 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims description 2
- SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 claims description 2
- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 claims description 2
- 229920002683 Glycosaminoglycan Polymers 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- YAPJXKKTHXODSV-LSCFUAHRSA-N [(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-4-hydroxy-2-[[hydroxy-[hydroxy(phosphonooxy)phosphoryl]oxyphosphoryl]oxymethyl]oxolan-3-yl] 2-(methylamino)benzoate Chemical compound CNC1=CC=CC=C1C(=O)O[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(N)=C3N=C2)O[C@@H]1COP(O)(=O)OP(O)(=O)OP(O)(O)=O YAPJXKKTHXODSV-LSCFUAHRSA-N 0.000 claims description 2
- JRMSLDWZFJZLAS-UHFFFAOYSA-M [7-(dimethylamino)-1,9-dimethylphenothiazin-3-ylidene]-dimethylazanium;chloride Chemical compound [Cl-].CC1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC(C)=C3N=C21 JRMSLDWZFJZLAS-UHFFFAOYSA-M 0.000 claims description 2
- 229940023020 acriflavine Drugs 0.000 claims description 2
- 150000003835 adenosine derivatives Chemical class 0.000 claims description 2
- 229960001456 adenosine triphosphate Drugs 0.000 claims description 2
- VYXSBFYARXAAKO-WTKGSRSZSA-N chembl402140 Chemical compound Cl.C1=2C=C(C)C(NCC)=CC=2OC2=C\C(=N/CC)C(C)=CC2=C1C1=CC=CC=C1C(=O)OCC VYXSBFYARXAAKO-WTKGSRSZSA-N 0.000 claims description 2
- 239000006071 cream Substances 0.000 claims description 2
- 239000013078 crystal Substances 0.000 claims description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 claims description 2
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthrene Natural products C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 claims description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 claims description 2
- FRIPRWYKBIOZJU-UHFFFAOYSA-N fluorone Chemical compound C1=CC=C2OC3=CC(=O)C=CC3=CC2=C1 FRIPRWYKBIOZJU-UHFFFAOYSA-N 0.000 claims description 2
- YFHXZQPUBCBNIP-UHFFFAOYSA-N fura-2 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=3OC(=CC=3C=2)C=2OC(=CN=2)C(O)=O)N(CC(O)=O)CC(O)=O)=C1 YFHXZQPUBCBNIP-UHFFFAOYSA-N 0.000 claims description 2
- PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- IOOMXAQUNPWDLL-UHFFFAOYSA-M lissamine rhodamine anion Chemical compound C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=C(S([O-])(=O)=O)C=C1S([O-])(=O)=O IOOMXAQUNPWDLL-UHFFFAOYSA-M 0.000 claims description 2
- XJCPMUIIBDVFDM-UHFFFAOYSA-M nile blue A Chemical compound [Cl-].C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4[O+]=C3C=C(N)C2=C1 XJCPMUIIBDVFDM-UHFFFAOYSA-M 0.000 claims description 2
- VOFUROIFQGPCGE-UHFFFAOYSA-N nile red Chemical compound C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=CC(=O)C2=C1 VOFUROIFQGPCGE-UHFFFAOYSA-N 0.000 claims description 2
- 239000002674 ointment Substances 0.000 claims description 2
- 150000004893 oxazines Chemical class 0.000 claims description 2
- 239000004177 patent blue V Substances 0.000 claims description 2
- 235000012736 patent blue V Nutrition 0.000 claims description 2
- 150000005053 phenanthridines Chemical class 0.000 claims description 2
- IEQIEDJGQAUEQZ-UHFFFAOYSA-N phthalocyanine Chemical compound N1C(N=C2C3=CC=CC=C3C(N=C3C4=CC=CC=C4C(=N4)N3)=N2)=C(C=CC=C2)C2=C1N=C1C2=CC=CC=C2C4=N1 IEQIEDJGQAUEQZ-UHFFFAOYSA-N 0.000 claims description 2
- NUSQOFAKCBLANB-UHFFFAOYSA-N phthalocyanine tetrasulfonic acid Chemical compound C12=CC(S(=O)(=O)O)=CC=C2C(N=C2NC(C3=CC=C(C=C32)S(O)(=O)=O)=N2)=NC1=NC([C]1C=CC(=CC1=1)S(O)(=O)=O)=NC=1N=C1[C]3C=CC(S(O)(=O)=O)=CC3=C2N1 NUSQOFAKCBLANB-UHFFFAOYSA-N 0.000 claims description 2
- 150000004033 porphyrin derivatives Chemical class 0.000 claims description 2
- 150000004032 porphyrins Chemical class 0.000 claims description 2
- 239000003755 preservative agent Substances 0.000 claims description 2
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims description 2
- LISFMEBWQUVKPJ-UHFFFAOYSA-N quinolin-2-ol Chemical compound C1=CC=C2NC(=O)C=CC2=C1 LISFMEBWQUVKPJ-UHFFFAOYSA-N 0.000 claims description 2
- 125000003698 tetramethyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 claims description 2
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical class [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims description 2
- 229930192474 thiophene Natural products 0.000 claims description 2
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims 1
- 238000002844 melting Methods 0.000 claims 1
- 230000008018 melting Effects 0.000 claims 1
- DHAHKSQXIXFZJB-UHFFFAOYSA-O patent blue V Chemical compound C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=C(O)C=1)S(O)(=O)=O)S(O)(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1 DHAHKSQXIXFZJB-UHFFFAOYSA-O 0.000 claims 1
- 230000002335 preservative effect Effects 0.000 claims 1
- 150000003732 xanthenes Chemical class 0.000 claims 1
- 206010052428 Wound Diseases 0.000 description 38
- 208000027418 Wounds and injury Diseases 0.000 description 38
- 210000001519 tissue Anatomy 0.000 description 27
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 18
- 229960000686 benzalkonium chloride Drugs 0.000 description 11
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 11
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 10
- 239000000975 dye Substances 0.000 description 10
- 229960001484 edetic acid Drugs 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000009472 formulation Methods 0.000 description 8
- 244000005700 microbiome Species 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 8
- 239000000499 gel Substances 0.000 description 7
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 6
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 6
- 238000001228 spectrum Methods 0.000 description 6
- 238000000862 absorption spectrum Methods 0.000 description 5
- 230000001154 acute effect Effects 0.000 description 5
- 229960001950 benzethonium chloride Drugs 0.000 description 5
- UREZNYTWGJKWBI-UHFFFAOYSA-M benzethonium chloride Chemical compound [Cl-].C1=CC(C(C)(C)CC(C)(C)C)=CC=C1OCCOCC[N+](C)(C)CC1=CC=CC=C1 UREZNYTWGJKWBI-UHFFFAOYSA-M 0.000 description 5
- 230000001684 chronic effect Effects 0.000 description 5
- 238000005286 illumination Methods 0.000 description 5
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- DLRVVLDZNNYCBX-UHFFFAOYSA-N Polydextrose Polymers OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(O)O1 DLRVVLDZNNYCBX-UHFFFAOYSA-N 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 229960005150 glycerol Drugs 0.000 description 4
- 235000011187 glycerol Nutrition 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 210000001015 abdomen Anatomy 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- 210000000416 exudates and transudate Anatomy 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 235000015277 pork Nutrition 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 238000012800 visualization Methods 0.000 description 3
- 230000029663 wound healing Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 229920001100 Polydextrose Polymers 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- LWZFANDGMFTDAV-BURFUSLBSA-N [(2r)-2-[(2r,3r,4s)-3,4-dihydroxyoxolan-2-yl]-2-hydroxyethyl] dodecanoate Chemical compound CCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O LWZFANDGMFTDAV-BURFUSLBSA-N 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 238000007373 indentation Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000001338 necrotic effect Effects 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 239000001259 polydextrose Substances 0.000 description 2
- 235000013856 polydextrose Nutrition 0.000 description 2
- 229940035035 polydextrose Drugs 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 2
- 210000004915 pus Anatomy 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 235000011067 sorbitan monolaureate Nutrition 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 229960002920 sorbitol Drugs 0.000 description 2
- 235000010356 sorbitol Nutrition 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- KFDNQUWMBLVQNB-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;sodium Chemical compound [Na].[Na].[Na].[Na].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KFDNQUWMBLVQNB-UHFFFAOYSA-N 0.000 description 1
- JCTYXESWNZITDY-UHFFFAOYSA-N 4-hexadecylmorpholine Chemical compound CCCCCCCCCCCCCCCCN1CCOCC1 JCTYXESWNZITDY-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 239000004251 Ammonium lactate Substances 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- RTMBGDBBDQKNNZ-UHFFFAOYSA-L C.I. Acid Blue 3 Chemical compound [Ca+2].C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=C(O)C=1)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1.C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=C(O)C=1)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1 RTMBGDBBDQKNNZ-UHFFFAOYSA-L 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- XMSXQFUHVRWGNA-UHFFFAOYSA-N Decamethylcyclopentasiloxane Chemical compound C[Si]1(C)O[Si](C)(C)O[Si](C)(C)O[Si](C)(C)O[Si](C)(C)O1 XMSXQFUHVRWGNA-UHFFFAOYSA-N 0.000 description 1
- 208000002064 Dental Plaque Diseases 0.000 description 1
- 208000008960 Diabetic foot Diseases 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- TZXKOCQBRNJULO-UHFFFAOYSA-N Ferriprox Chemical compound CC1=C(O)C(=O)C=CN1C TZXKOCQBRNJULO-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000005230 Leg Ulcer Diseases 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- UBQYURCVBFRUQT-UHFFFAOYSA-N N-benzoyl-Ferrioxamine B Chemical compound CC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCN UBQYURCVBFRUQT-UHFFFAOYSA-N 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 208000004210 Pressure Ulcer Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 125000005211 alkyl trimethyl ammonium group Chemical group 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 229940059265 ammonium lactate Drugs 0.000 description 1
- 235000019286 ammonium lactate Nutrition 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003214 anti-biofilm Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 125000006615 aromatic heterocyclic group Chemical group 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- RZOBLYBZQXQGFY-HSHFZTNMSA-N azanium;(2r)-2-hydroxypropanoate Chemical compound [NH4+].C[C@@H](O)C([O-])=O RZOBLYBZQXQGFY-HSHFZTNMSA-N 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229960004106 citric acid Drugs 0.000 description 1
- 229940086555 cyclomethicone Drugs 0.000 description 1
- 229960001489 deferasirox Drugs 0.000 description 1
- FMSOAWSKCWYLBB-VBGLAJCLSA-N deferasirox Chemical compound C1=CC(C(=O)O)=CC=C1N(N\C(N\1)=C\2C(C=CC=C/2)=O)C/1=C\1C(=O)C=CC=C/1 FMSOAWSKCWYLBB-VBGLAJCLSA-N 0.000 description 1
- 229960003266 deferiprone Drugs 0.000 description 1
- 229960000958 deferoxamine Drugs 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- IQDGSYLLQPDQDV-UHFFFAOYSA-N dimethylazanium;chloride Chemical compound Cl.CNC IQDGSYLLQPDQDV-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- WSDISUOETYTPRL-UHFFFAOYSA-N dmdm hydantoin Chemical compound CC1(C)N(CO)C(=O)N(CO)C1=O WSDISUOETYTPRL-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 229960001617 ethyl hydroxybenzoate Drugs 0.000 description 1
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical group CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 229920000912 exopolymer Polymers 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 239000001046 green dye Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 239000001863 hydroxypropyl cellulose Substances 0.000 description 1
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000000845 maltitol Substances 0.000 description 1
- VQHSOMBJVWLPSR-WUJBLJFYSA-N maltitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-WUJBLJFYSA-N 0.000 description 1
- 235000010449 maltitol Nutrition 0.000 description 1
- 229940035436 maltitol Drugs 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 229960001855 mannitol Drugs 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920000193 polymethacrylate Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 229920002689 polyvinyl acetate Polymers 0.000 description 1
- 239000011118 polyvinyl acetate Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 description 1
- 229960003415 propylparaben Drugs 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 125000001834 xanthenyl group Chemical class C1=CC=CC=2OC3=CC=CC=C3C(C12)* 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
- A61K49/0041—Xanthene dyes, used in vivo, e.g. administered to a mice, e.g. rhodamines, rose Bengal
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Definitions
- This invention relates to a composition which can be applied to viable tissues such as chronic wounds (e.g. leg ulcers, pressure ulcers, diabetic foot ulcers), acute wounds (e.g. cuts, abrasions, burns), skin and bone for the detection of microbial biofilms as an early warning indicator for tissue at risk of infection. More particularly the invention relates to a composition capable of making biofilms on viable tissues observable while at the same time avoiding wound and skin irritation and retardation of healing. A further embodiment of the invention relates to a kit for use in the detection of biofilms on viable tissues.
- viable tissues such as chronic wounds (e.g. leg ulcers, pressure ulcers, diabetic foot ulcers), acute wounds (e.g. cuts, abrasions, burns), skin and bone for the detection of microbial biofilms as an early warning indicator for tissue at risk of infection.
- viable tissues such as chronic wounds (e.g. leg ulcers, pressure ulcers, diabetic foot ulcers), acute wounds (e.g
- Viable tissues are often colonised by a variety of microorganisms, some of which may cause infection. It is becoming increasingly accepted that chronic and acute wound healing is impaired by the presence of microorganisms colonising the wound. Compelling evidence is emerging that these microorganisms may exist in wounds primarily in the form of biofilms. During colonisation, bacteria and other microorganisms such as yeasts and fungi, attach firmly to tissue and form biofilm via the secretion of an extracellular matrix of polymeric substances. This mode of growth imparts a degree of protection to microorganisms within the biofilm in the form of physical protection from topical and systemic antimicrobial agents due to the surrounding matrix.
- Biofilm microorganisms within biofilms have altered phenotypes and genotypes compared to their free-swimming, planktonic counterparts.
- Biofilm microorganisms are known to be metabolically less active, and as such this may also provide a degree of resistance to traditional antimicrobial approaches such as antibiotics, which are known to work against metabolically-active bacteria.
- antibiotics which are known to work against metabolically-active bacteria.
- the presence of biofilms in wounds also impedes the host immune system in the inflammatory microbial clearance, and the granulation and re-epithelialisation phases, of the normal wound healing process.
- biofilm detection method or device would also allow researchers to develop effective anti-biofilm strategies and effective wound healing protocols of care, and in clinical practice, it would guide selection of appropriate wound dressings and aid the monitoring of the effectiveness of a treatment protocol given by the wound care practitioner.
- Biofilms are typically comprised of bacteria encased within an exopolymeric substance (or matrix) that consists of long-chain polysaccharides with complex linkages such as 1,3- or 1,4- ⁇ -linked hexose linkages (examples of some common biofilm polysaccharides are teichoic acid, ketal-linked pryruvates N-acetylglucosamine, and the uronic acids: D-guluronic, D-galacturonic and mannuronic acids), protein (of which some may play a structural role), DNA (extracellular, some of which may have a structural role), lipids, metal ions (Ca 2+ , Mg 2+ , Fe 3+ , etc.) and water. Biofilms may also be associated with devitalised host tissues such as slough and necrotic tissue.
- Biofilm extracellular polymeric substances are also referred to as biofilm extracellular matrix or bacterial-derived tissue. Whilst biofilm is mainly water by weight and bacteria may only comprise 10-20% of the volume of biofilm, EPS constitutes the majority of the biomass/dry weight of biofilm.
- Biofilms such as those found on teeth in the form of dental plaque, are often easy to visualise with the naked eye due to their thickness, colour and the nature of the substrate on which they form. Biofilm visualisation in, for example, a chronic or acute wound is not straightforward due to the colours present in the wound and the contents of the wound. Chronic and acute wounds are usually complex in terms of containing dead or devitalised tissue (slough), exudate, pus, blood, medicaments, dressing components, in addition to bacteria and biofilm. As such it may be difficult to detect the presence of a biofilm in a wound as the visualisation of wound biofilms by the naked eye is difficult. There is thus a need for a means to aid detection of the biofilm for instance by a composition which is able to preferentially stain wound biofilms so that they can be visualised. Once visualised, the biofilm can be treated appropriately.
- a first aspect of the invention provides a staining composition for use in making biofilm on viable tissue detectable wherein the composition preferentially stains the biofilm and comprises a coloured or fluorescent staining agent in solution in a quantity effective to stain said biofilm and render it detectable.
- the biofilm is made detectable by the naked eye or in conjunction with illumination and/or optical devices.
- the staining agent is a dye which will selectively bind to EPS/bacteria and not to host viable tissue.
- the staining agent should not significantly stain non-biofilm components in, for example, a chronic or acute wound, such as wound tissue, surrounding skin, slough (dead or devitalized tissue), exudate, blood, pus, inflammatory cells (neutrophils, macrophages), cells involved in the healing process (fibroblasts, endothelial cells, keratinocytes), or medicaments or dressing components that may remain in the wound.
- Such a staining agent would preferably be a molecule of the required size and structure; for example, a low molecular weight, compact, planar molecule that is capable of diffusion through EPS and bacterial cell membranes.
- the staining agent may have anionic groups to give colour and possibly fluorescent properties; and may have cationic groups for charge interactions with negatively-charged biofilm EPS polysaccharides and bacterial cell walls. These charged groups should preferably be permanent charges so that they are not affected by the pH of applied formulations or the viable tissue/biofilm environment.
- Such a staining agent is preferably of a suitable colour or brightness that it may be possible to observe the stained biofilm directly with the naked eye.
- some colours of stain might be difficult to observe.
- hues of black, brown, red, yellow, etc. Whilst blue or green dyes may be considered preferable here, they may appear dark or black if located adjacent to or on brown, red or yellow-coloured tissue. Rather than relying solely on the observer being able to detect the stain with the naked eye, it is preferable to use a stain that can fluoresce so that it is more readily detectable.
- Many classes of compounds that are potential staining agents are also fluorescent, in that they are capable of absorbing photons of light of certain wavelengths and becoming electronically excited, emitting this light energy in the form of fluorescence. It is possible to detect such fluorescence using light filters that are tailored to the fluorescence emission spectra of relevant molecules so that the observer only views a narrow spectral band that corresponds to the wavelengths of fluorescence. For example, lenses with specific optical filters, such as those used in laser safety, could be used in conjunction with an appropriately-specific light source to detect fluorescence, thereby allowing detection of biofilm on viable tissues. This method of biofilm detection may have advantages over detection using the naked eye in that fluorescent detection is possible even for very thin biofilms, which may be only a few layers of microbial cells thick and does not rely solely on the stain itself being visible.
- the staining agent selectively binds to the biofilm rather than the host viable tissue.
- the staining agent can be used simply to detect the biofilm by revealing the presence and location the biofilm.
- the staining agent becomes bound or adsorbed to extracellular biofilm matrix molecules as well as being bound or adsorbed to and/or taken up by the biofilm bacteria cells rather than the tissue of the wound.
- the staining of the biofilm by the staining agent reveals the presence of biofilm by making it detectable to the naked eye.
- the stained biofilm can be made to fluoresce, for instance by illumination with a light source. The fluorescence can make the stained biofilm more visible.
- the light source is selected to emit light of an appropriate wavelength such that the staining agent absorbs light energy in the form of photons to excite the staining agent and cause it to fluoresce.
- the observation of fluorescence may be enhanced using appropriate optical filters which exclude non-fluorescent wavelengths for the staining agent, for instance in the form of optically-filtered lenses.
- compositions according to a first aspect of the invention comprise one or more staining agents capable of preferentially staining biofilms.
- the staining agent is a dye, the biofilm-staining dye absorbing maximally in the visible region, more preferably 380 nm to 720 nm and even more preferably 500 nm to 600 nm, as this makes a wide range of light sources suitable for use with the composition of the invention or for inclusion in the kit of the invention.
- the staining agent produces sufficient fluorescence for detection, such that a wavelength range suitable for excitation and a distinct and higher wavelength range suitable for fluorescence emission detection can be delivered from illumination devices and detected using optically-filtered lens devices.
- Staining agents suitable for use in compositions of the invention are most preferably selected from those based upon organic chemical compounds, more likely to be those containing aromatic ring structures, for example benzene, or extended conjugation, for example in porphyrin or pheophorbides, more likely those with fused polycyclic hydrocarbons, for example naphthalene, anthracene, phenanthraline and pyrene, and most likely those also containing heterocyclic aromatic structures where the heterocyclic atom or atoms are oxygen, nitrogen or sulphur, or a mixture thereof, for example furan, thiophene, pyrrole, pyran, pyridine and oxazine.
- aromatic ring structures for example benzene, or extended conjugation, for example in porphyrin or pheophorbides
- fused polycyclic hydrocarbons for example naphthalene, anthracene, phenanthraline and pyrene
- staining agents may also display the properties of fluorescence. Most appropriate are those that absorb and emit light of wavelengths in the visible electromagnetic spectrum (380 nm-720 nm). Such agents are likely to have chemical structures that contain an extended region of conjugation, for example fluorone and its derivatives (alternatively known as xanthenes and rhodamines) such as eosin, erythrosine, Rose Bengal, fluorescein-5-isothiocyanate, 5-chloromethyl fluorescein, 6-carboxy fluorescein, 2,7-Bis-(carboxy ethyl)-5(6)-carboxy fluorescein; Rhodamine B, Rhodamine 6G, Rhodamine 123, Rhodamine iodoacetamide, Sulphorhodamine B, Sulphorhodamine 101, tetramethyl rhodamines or Texas Red.
- fluorone and its derivatives such as eosin, erythrosine
- cyanine such as 3,3′-dihexadecylindocarbocyanines, 3,3′-dipropyloxadicrbocyanine, aluminium phthalocyanine disulphonate, aluminium tetrasulphophthalocyanine, aluminium phthalocyanines, zinc phthalocyanines, napthalocyanines, or the mucopolysaccharide stain Alcian Blue.
- Acridine and its derivatives such as Acriflavine, Aminacrine, 2-aminoacridine or 9-aminoacridine.
- appropriate agents may come from the classes of oxazine derivatives such as Nile Blue, Nile Red, Fura Red or Fura-2; quinolone and its derivatives; adenosine derivatives such as 2′3′-O-(2,4,6-trinotro-cyclohexadienylidine)adenosine 5′-triphosphate or 3′-O-(N-methylanthraniloyl)adenosine 5′-triphosphate; triarylmethanes such as Patent Blue V, Crystal Violet, Brilliant Blue or Fast Green; 5 phenothiaziniums such as Methylene Blue or Toluidine Blue O and its derivatives such as 1-methyl methylene blue or 1,9-dimethyl methylene blue; phenanthridine derivatives such as ethidium or hydroethidine; pheophorbide derivatives such as sodium pheophorbide; and porphyrin derivatives such as chlorin e6, benzoporphyrin derivatives, por
- the staining agent is preferably included in the composition at a level of from 0.0001% to 1% by weight, more preferably 0.0025% to 0.025% by weight, even more preferably 0.0025% to 0.01% by weight.
- compositions of the present invention may be in a form that lightly adheres to tissues and may be readily rinsed away after a short duration to aid visualisation of the stained biofilm.
- a viscous fluid for instance a water- or glycerol-based gel (in the form of a gel applicator, spray or sheet), foaming mousse, cream or ointment would give intimate contact with a wound bed.
- a thin, soluble, cast film or a lyophilized, dissolving wafer could be used to provide intimate contact with a wound bed.
- the formulation preferably should be easily rinsed away from viable tissues using a standard irrigant such as saline, for a few seconds.
- the composition may also comprise a viscosifier such as a cellulose derivative such hydroxyethyl cellulose (HEC), carboxymethyl cellulose or hydroxypropyl cellulose; gums; sugar/alcohol derivatives such as glycerol, sorbitol, maltitol, mannitol, maltodextrin or polydextrose; natural polymers such as gelatin, pectin, chitosan or alginate; synthetic polymers such as carbopol, polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol, polyacrylate, polymethacrylate, polyethylene glycol or poloxamers.
- a viscosifier such as a cellulose derivative such hydroxyethyl cellulose (HEC), carboxymethyl cellulose or hydroxypropyl cellulose; gums; sugar/alcohol derivatives such as glycerol, sorbitol, maltitol, mannitol, maltodextrin or
- composition of the invention may be in gel form and may also comprise a humectant such as propylene glycol (PG), glycerol, polyethylene glycol, polydextrose, sorbitol, triethanlolamine, cyclomethicone, ammonium lactate or glycerol ester.
- a humectant such as propylene glycol (PG), glycerol, polyethylene glycol, polydextrose, sorbitol, triethanlolamine, cyclomethicone, ammonium lactate or glycerol ester.
- PG propylene glycol
- glycerol polyethylene glycol
- polydextrose polydextrose
- sorbitol triethanlolamine
- cyclomethicone triethanlolamine
- ammonium lactate or glycerol ester.
- the composition comprises from 5% to 15% by weight of a humectant and most preferably PG.
- the composition of the invention comprises excipients to optimise binding of the stain to biofilm.
- the composition of the invention may also comprise a metal chelating agent such as tetra sodium ethylene diamine tetraacetic acid (EDTA), citric acid, deferasirox, deferiprone, deferoxamine, deferazoxane, ethylene glycol tetraacetic acid, gluonic acid, nitrilotriacetic acid or trisodium citrate at a level of 0.1 to 2.0% by weight, or an agent to assist penetration of the staining agent into the biofilm such as a surfactant for example Tween 80, Span 20, or coamidopropylbetaine (CAPD) and in particular a cationic quaternary ammonium surfactant such as dialkyl dimethyl ammonium chloride, alkyl pyridinium chloride, benzalkonium chloride (BaCl), benzethonium chloride (BeCl),
- a surfactant may also act as a foaming agent.
- the surfactants BeCl, Tween 80, Span 20, CAPD, sodium C 14-16 olefin sulfonate or Softisan 649 can act as foaming agents to give the formulation characteristics of a foaming mousse.
- composition of the invention has a pH in the range of from 5 to 7 and most preferably around 5.5.
- the composition of the invention is in the form of a viscous fluid and comprises a viscosifier such as HEC, a humectant such as PG, a metal chelator such as EDTA, a surfactant such as BeCl and water and in particular 2.0% w/v hydroxyethylcellulose, 10.0% w/v propylene glycol, 0.5% EDTA, 0.5% BeCl and approximately 87% v/v sterile, distilled water.
- the compositions of the present invention could be in the form of a solution applied to the wound from a syringe, sachet, spray bottle, aerosol bottle, non-propellant pump bottle, brush or a gel sheet, film or dissolving wafer.
- the formulation could be terminally sterilized by autoclaving or gamma irradiation.
- the formulation could be a preserved solution containing, for example preservatives such as DMDM hydantoin or parabens such as methyl-, ethyl- or propyl-paraben.
- composition of the present invention will be used primarily on viable tissues which show signs of clinical infection (inflammation, malodour, purulent exudate, hypoxia, etc.), may be at risk of infection, appear to have slough (host-derived tissue) or biofilm (bacteria-derived tissue) present, or are generally recalcitrant.
- the composition could also be used at dressing change, in order to detect biofilm, and also to monitor the efficacy of the treatment regime and direct future treatment via the reduction in detected biofilm.
- a further aspect of the present invention relates to a kit of parts for use in the detection of biofilms on viable tissue, the kit comprising:
- kit further comprises optical filters to enable the specific detection of fluorescence in the form of spectacles or integrated optical filters.
- the light source may be a white light source such as tungsten, halogen or pulsed xenon lamp that is passed through a “short pass” filter.
- the light source is a monochromatic or narrow spectrum source that does not require filtering or attenuation such as a light emitting diode with an output that closely correlates with the spectral characteristics of the staining agent.
- the light source is preferably small, portable, hand-held and generates no or negligible heat.
- the light source may be multiple use or fully disposable.
- the kit further comprises spectacles, or the light source contains an integrated lens, for use in detecting the biofilm wherein the spectacles or lens contain a filter to exclude all wavelengths of light below the absorption maxima (Abs max ) of the staining agent.
- the spectacles or lens filter is efficient at transmitting wavelengths of light corresponding to the fluorescence emission spectra of the staining agent.
- the spectacles can be multiple use or fully disposable (in the same way that an integrated lens in the light source could be).
- the kit further comprises a wound irrigation solution for rinsing the viable tissue before and/or after illumination with the light source.
- a composition according to the invention is applied to the whole wound or desired regions in order to achieve a thin but consistent layer of composition for instance 0.1 to 0.5 cm in thickness.
- the composition is left in place for 0.1 to 15 minutes, more preferably 0.5 to 2 minutes.
- the formulation Prior to any illumination, the formulation can be left in place or more preferably excess formulation is rinsed away from the wound using a suitable wound irrigation solution. Rinsing the excess formulation away can enable the staining to show where the biofilm is in the wound so that these areas can be treated for example by curette.
- the wound is then inspected for presence of preferentially stained biofilm.
- This may be done with the naked eye or the wound can be illuminated with a light source at a distance in the range of 1 to 50 cm.
- the light source is at a distance of 5 to 20 cm.
- the light source spectral output is selected to correspond to the fluorescence emission spectra of the staining agent; for example, for Rose Bengal, which has a fluorescence maxima of 575 nm, a suitable light source might emit wavelengths of 550-600 nm. This causes the agent in the stained biofilm to fluoresce.
- the user wears spectacles to observe the wound or observes the wound through an integrated lens in the light source.
- the spectacles or lens have a light filtration system which excludes wavelengths of light below the fluorescence emission range of the staining agent and allows any fluorescence to be seen remarkably clearly and specifically and thus, detection of any biofilm present.
- treatment should take place at subsequent dressing changes.
- the wound can be further inspected for presence and reduction of biofilm with the naked eye or with the light source and spectacles or integrated lens.
- the wound can then be dressed with an appropriate primary and secondary dressing.
- FIG. 3 Rose Bengal with EDTA and BaCl (RBEB) stained (A-C) and control (D-E) samples of meat containing mixed S. aureus and P. aeruginosa biofilms.
- RBEB Rose Bengal with EDTA and BaCl
- FIG. 4 S. aureous biofilm age vs dye concentration.
- FIG. 5 P. aeruginosa biofilm age vs Rose Bengal concentration.
- a constant depth biofilm fermenter (CDFF) was used to culture 4-day mixed biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. Briefly, biofilms were formed in recesses in 15 PTFE pans around the rim of a rotating steel turntable onto which inoculum or sterile growth media was allowed to steadily drip. A scraper bar distributed bacterial inoculum or media over the pans as the turntable rotated, maintaining the biofilms at a steady depth. Each pan contained 5 removable plugs, 4 mm in diameter which were recessed to a depth of 300 ⁇ m.
- biofilm-containing pans were removed from the CDFF, rinsed once by dipping in sterile saline for 5 seconds, then incubated in 10 ml volumes of the following potential biofilm stains at 100 ⁇ M concentration in deionised water unless stated, in the dark for two minutes: Erythrosine; Rose Bengal; Fast Green; Rose Bengal with 2% w/v EDTA and 1% w/v benzalkonium chloride (BaCl) (RBEB); Rose Bengal with 2% w/v tetra sodium EDTA and 1% w/v benzalkonium chloride in a 2% w/v hydroxyethyl cellulose and 10% w/v propylene glycol gel (Gel-RBEB).
- FIG. 1 shows that Fast Green appeared to be the most effective stain of biofilms grown in the CDFF, closely followed by Rose Bengal with EDTA and BaCl in liquid form. The addition of the EDTA and BaCl excipients appeared to enhance the uptake of Rose Bengal into the biofilms.
- a Centre for Disease Control (CDC) biofilm reactor was used to culture 48hour mixed biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. Briefly, biofilms were formed on coupons on reactor rods which were held within the reactor vessel containing a continuously mixed culture of S. aureus and P. aeruginosa at 35° C.
- Biofilm-containing coupons were removed from the rods, rinsed once by dipping in sterile saline for 5 seconds, then incubated in 10 ml volumes of the following biofilm stains at 100 ⁇ M concentration in deionised water, in the dark for two minutes: Erythrosine; Rose Bengal; Alcian Blue; Rhodamine B; Rhodamine 123; Rose Bengal with 2% w/v EDTA and 1% w/v BaCl (RBEB). Following another rinse in saline, nine coupons for each stain were each added to 2 ml volumes of 2% SDS for stomaching at ‘high’ setting for 1 minute then incubated overnight in the dark at 37° C., for digestion.
- FIG. 2 a shows how in terms of absolute absorption or ‘brightness’, RBEB was the most efficient biofilm stain, followed by the carbohydrate stain, Alcian Blue.
- the EDTA and/or BaCl appeared to enhance the uptake of Rose Bengal by more than 60%.
- Alcian Blue appeared to be the most effective biofilm stain.
- a pork belly biofilm model was used to further assess biofilm stains.
- Pieces of pork belly were cut using a 20 mm bore and a 6 mm borer was used to create indentations in the centre of the samples. Samples were then sterilized by gamma irradiation. Samples were inoculated with 10 ⁇ l volumes of a ⁇ 1 ⁇ 10 7 cfu/ml mixed suspension of S. aureus and P. aeruginosa then incubated at 35° C. in Parafilm-sealed Petri dishes for 72-96 hours.
- a membrane filter biofilm model was used to study the effect of Rose Bengal concentration on the efficacy of biofilm staining Briefly, a 5 ⁇ l volume of a 5 ⁇ 10 5 cfu/ml suspension of S. aureus or P. aeruginosa was added to the centre of sterile membrane filter discs (pore size 0.2 ⁇ m; Anodisc, Whatman) which were placed onto 7 ml volumes of sterile Tryptic Soya Broth in lidded 6-well plates. Samples were incubated for 4 hours (immature biofilms), and 24 and 48 hours (mature biofilms), and excess planktonic cells were rinsed from the filters.
- the biofilms on the filters were stained by pipetting 2 ml volumes of Rose Bengal (60 ⁇ M or 300 ⁇ M) or saline (negative control) for 30 seconds, followed by rinsing.
- Rose Bengal was recovered from the biofilm samples by stomaching and overnight digestion in 2% sodium dodecyl sulphate, centrifugation, then measuring of absorption spectra in a UV-vis spectrophotometer.
- Rose Bengal uptake per sample was determined by comparison of absorption values with a standard curve of Rose Bengal in 2% SDS. Tables 1 and 2 show that mature S. aureus and P. aeruginosa biofilms took up Rose Bengal in a dye concentration- and biofilm age-dependent manner.
- FIGS. 4 and 5 show how mature, 48-hour biofilms of S. aureus and P. aeruginosa took up more Rose Bengal than 24-hour biofilms, and also that 300 ⁇ M Rose Bengal resulted in significantly more staining of the biofilms than 60 ⁇ M Rose Bengal.
- This simple method for quantifying the uptake of Rose Bengal by biofilms utilises the absorption spectra of the dye. Using the absorption spectra to quantify the dye is similar to detecting the fluorescence using a light source with optical filters. Uptake could then alternatively be observed qualitatively using the naked eye or by observing the fluorescence emission of the Rose Bengal in conjunction with a light source and optical filter.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
- This invention relates to a composition which can be applied to viable tissues such as chronic wounds (e.g. leg ulcers, pressure ulcers, diabetic foot ulcers), acute wounds (e.g. cuts, abrasions, burns), skin and bone for the detection of microbial biofilms as an early warning indicator for tissue at risk of infection. More particularly the invention relates to a composition capable of making biofilms on viable tissues observable while at the same time avoiding wound and skin irritation and retardation of healing. A further embodiment of the invention relates to a kit for use in the detection of biofilms on viable tissues.
- Viable tissues are often colonised by a variety of microorganisms, some of which may cause infection. It is becoming increasingly accepted that chronic and acute wound healing is impaired by the presence of microorganisms colonising the wound. Compelling evidence is emerging that these microorganisms may exist in wounds primarily in the form of biofilms. During colonisation, bacteria and other microorganisms such as yeasts and fungi, attach firmly to tissue and form biofilm via the secretion of an extracellular matrix of polymeric substances. This mode of growth imparts a degree of protection to microorganisms within the biofilm in the form of physical protection from topical and systemic antimicrobial agents due to the surrounding matrix. It is also thought that microorganisms within biofilms have altered phenotypes and genotypes compared to their free-swimming, planktonic counterparts. Biofilm microorganisms are known to be metabolically less active, and as such this may also provide a degree of resistance to traditional antimicrobial approaches such as antibiotics, which are known to work against metabolically-active bacteria. Furthermore, the presence of biofilms in wounds also impedes the host immune system in the inflammatory microbial clearance, and the granulation and re-epithelialisation phases, of the normal wound healing process.
- Consequently, there is a need to develop methods or devices to rapidly detect the presence of biofilm in viable tissues before and after selected treatment protocols. This would help researchers to understand if microorganisms live in the biofilm state on skin and in wounds, and to allow them to follow maturation or clearance of such biofilm communities. A biofilm detection method or device would also allow researchers to develop effective anti-biofilm strategies and effective wound healing protocols of care, and in clinical practice, it would guide selection of appropriate wound dressings and aid the monitoring of the effectiveness of a treatment protocol given by the wound care practitioner.
- Biofilms are typically comprised of bacteria encased within an exopolymeric substance (or matrix) that consists of long-chain polysaccharides with complex linkages such as 1,3- or 1,4-β-linked hexose linkages (examples of some common biofilm polysaccharides are teichoic acid, ketal-linked pryruvates N-acetylglucosamine, and the uronic acids: D-guluronic, D-galacturonic and mannuronic acids), protein (of which some may play a structural role), DNA (extracellular, some of which may have a structural role), lipids, metal ions (Ca2+, Mg2+, Fe3+, etc.) and water. Biofilms may also be associated with devitalised host tissues such as slough and necrotic tissue.
- Biofilm extracellular polymeric substances (EPS) are also referred to as biofilm extracellular matrix or bacterial-derived tissue. Whilst biofilm is mainly water by weight and bacteria may only comprise 10-20% of the volume of biofilm, EPS constitutes the majority of the biomass/dry weight of biofilm.
- Biofilms such as those found on teeth in the form of dental plaque, are often easy to visualise with the naked eye due to their thickness, colour and the nature of the substrate on which they form. Biofilm visualisation in, for example, a chronic or acute wound is not straightforward due to the colours present in the wound and the contents of the wound. Chronic and acute wounds are usually complex in terms of containing dead or devitalised tissue (slough), exudate, pus, blood, medicaments, dressing components, in addition to bacteria and biofilm. As such it may be difficult to detect the presence of a biofilm in a wound as the visualisation of wound biofilms by the naked eye is difficult. There is thus a need for a means to aid detection of the biofilm for instance by a composition which is able to preferentially stain wound biofilms so that they can be visualised. Once visualised, the biofilm can be treated appropriately.
- Surprisingly we have found that it is possible to preferentially stain biofilms by the use of a composition comprising a stain which allows the detection of biofilm.
- Accordingly a first aspect of the invention provides a staining composition for use in making biofilm on viable tissue detectable wherein the composition preferentially stains the biofilm and comprises a coloured or fluorescent staining agent in solution in a quantity effective to stain said biofilm and render it detectable.
- Preferably the biofilm is made detectable by the naked eye or in conjunction with illumination and/or optical devices.
- Preferably the staining agent is a dye which will selectively bind to EPS/bacteria and not to host viable tissue. The staining agent should not significantly stain non-biofilm components in, for example, a chronic or acute wound, such as wound tissue, surrounding skin, slough (dead or devitalized tissue), exudate, blood, pus, inflammatory cells (neutrophils, macrophages), cells involved in the healing process (fibroblasts, endothelial cells, keratinocytes), or medicaments or dressing components that may remain in the wound. Such a staining agent would preferably be a molecule of the required size and structure; for example, a low molecular weight, compact, planar molecule that is capable of diffusion through EPS and bacterial cell membranes. The staining agent may have anionic groups to give colour and possibly fluorescent properties; and may have cationic groups for charge interactions with negatively-charged biofilm EPS polysaccharides and bacterial cell walls. These charged groups should preferably be permanent charges so that they are not affected by the pH of applied formulations or the viable tissue/biofilm environment.
- Such a staining agent is preferably of a suitable colour or brightness that it may be possible to observe the stained biofilm directly with the naked eye. However, due to the complex and highly pigmented nature of wounds some colours of stain might be difficult to observe. For example, in necrotic wounds, sloughy wounds, or bleeding wounds there may be hues of black, brown, red, yellow, etc. Whilst blue or green dyes may be considered preferable here, they may appear dark or black if located adjacent to or on brown, red or yellow-coloured tissue. Rather than relying solely on the observer being able to detect the stain with the naked eye, it is preferable to use a stain that can fluoresce so that it is more readily detectable. Many classes of compounds that are potential staining agents are also fluorescent, in that they are capable of absorbing photons of light of certain wavelengths and becoming electronically excited, emitting this light energy in the form of fluorescence. It is possible to detect such fluorescence using light filters that are tailored to the fluorescence emission spectra of relevant molecules so that the observer only views a narrow spectral band that corresponds to the wavelengths of fluorescence. For example, lenses with specific optical filters, such as those used in laser safety, could be used in conjunction with an appropriately-specific light source to detect fluorescence, thereby allowing detection of biofilm on viable tissues. This method of biofilm detection may have advantages over detection using the naked eye in that fluorescent detection is possible even for very thin biofilms, which may be only a few layers of microbial cells thick and does not rely solely on the stain itself being visible.
- By preferentially staining it is meant that the staining agent selectively binds to the biofilm rather than the host viable tissue. In this way, the staining agent can be used simply to detect the biofilm by revealing the presence and location the biofilm. The staining agent becomes bound or adsorbed to extracellular biofilm matrix molecules as well as being bound or adsorbed to and/or taken up by the biofilm bacteria cells rather than the tissue of the wound. Preferably the staining of the biofilm by the staining agent reveals the presence of biofilm by making it detectable to the naked eye. For some staining agents the stained biofilm can be made to fluoresce, for instance by illumination with a light source. The fluorescence can make the stained biofilm more visible. The light source is selected to emit light of an appropriate wavelength such that the staining agent absorbs light energy in the form of photons to excite the staining agent and cause it to fluoresce. The observation of fluorescence may be enhanced using appropriate optical filters which exclude non-fluorescent wavelengths for the staining agent, for instance in the form of optically-filtered lenses.
- The compositions according to a first aspect of the invention comprise one or more staining agents capable of preferentially staining biofilms. Preferably the staining agent is a dye, the biofilm-staining dye absorbing maximally in the visible region, more preferably 380 nm to 720 nm and even more preferably 500 nm to 600 nm, as this makes a wide range of light sources suitable for use with the composition of the invention or for inclusion in the kit of the invention.
- Preferably the staining agent produces sufficient fluorescence for detection, such that a wavelength range suitable for excitation and a distinct and higher wavelength range suitable for fluorescence emission detection can be delivered from illumination devices and detected using optically-filtered lens devices.
- Staining agents suitable for use in compositions of the invention are most preferably selected from those based upon organic chemical compounds, more likely to be those containing aromatic ring structures, for example benzene, or extended conjugation, for example in porphyrin or pheophorbides, more likely those with fused polycyclic hydrocarbons, for example naphthalene, anthracene, phenanthraline and pyrene, and most likely those also containing heterocyclic aromatic structures where the heterocyclic atom or atoms are oxygen, nitrogen or sulphur, or a mixture thereof, for example furan, thiophene, pyrrole, pyran, pyridine and oxazine.
- These staining agents may also display the properties of fluorescence. Most appropriate are those that absorb and emit light of wavelengths in the visible electromagnetic spectrum (380 nm-720 nm). Such agents are likely to have chemical structures that contain an extended region of conjugation, for example fluorone and its derivatives (alternatively known as xanthenes and rhodamines) such as eosin, erythrosine, Rose Bengal, fluorescein-5-isothiocyanate, 5-chloromethyl fluorescein, 6-carboxy fluorescein, 2,7-Bis-(carboxy ethyl)-5(6)-carboxy fluorescein; Rhodamine B, Rhodamine 6G, Rhodamine 123, Rhodamine iodoacetamide, Sulphorhodamine B, Sulphorhodamine 101, tetramethyl rhodamines or Texas Red. Derivatives of cyanine such as 3,3′-dihexadecylindocarbocyanines, 3,3′-dipropyloxadicrbocyanine, aluminium phthalocyanine disulphonate, aluminium tetrasulphophthalocyanine, aluminium phthalocyanines, zinc phthalocyanines, napthalocyanines, or the mucopolysaccharide stain Alcian Blue. Acridine and its derivatives such as Acriflavine, Aminacrine, 2-aminoacridine or 9-aminoacridine. Finally, appropriate agents may come from the classes of oxazine derivatives such as Nile Blue, Nile Red, Fura Red or Fura-2; quinolone and its derivatives; adenosine derivatives such as 2′3′-O-(2,4,6-trinotro-cyclohexadienylidine)adenosine 5′-triphosphate or 3′-O-(N-methylanthraniloyl)adenosine 5′-triphosphate; triarylmethanes such as Patent Blue V, Crystal Violet, Brilliant Blue or Fast Green; 5 phenothiaziniums such as Methylene Blue or Toluidine Blue O and its derivatives such as 1-methyl methylene blue or 1,9-dimethyl methylene blue; phenanthridine derivatives such as ethidium or hydroethidine; pheophorbide derivatives such as sodium pheophorbide; and porphyrin derivatives such as chlorin e6, benzoporphyrin derivatives, porphines, 10 meso-tetra porphines, hematoporphyrins or protoporphyrins.
- The staining agent is preferably included in the composition at a level of from 0.0001% to 1% by weight, more preferably 0.0025% to 0.025% by weight, even more preferably 0.0025% to 0.01% by weight.
- The compositions of the present invention may be in a form that lightly adheres to tissues and may be readily rinsed away after a short duration to aid visualisation of the stained biofilm. A viscous fluid, for instance a water- or glycerol-based gel (in the form of a gel applicator, spray or sheet), foaming mousse, cream or ointment would give intimate contact with a wound bed. Alternatively, a thin, soluble, cast film or a lyophilized, dissolving wafer could be used to provide intimate contact with a wound bed. In any such delivery system the formulation preferably should be easily rinsed away from viable tissues using a standard irrigant such as saline, for a few seconds.
- In gel form, the composition may also comprise a viscosifier such as a cellulose derivative such hydroxyethyl cellulose (HEC), carboxymethyl cellulose or hydroxypropyl cellulose; gums; sugar/alcohol derivatives such as glycerol, sorbitol, maltitol, mannitol, maltodextrin or polydextrose; natural polymers such as gelatin, pectin, chitosan or alginate; synthetic polymers such as carbopol, polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol, polyacrylate, polymethacrylate, polyethylene glycol or poloxamers. Preferably the composition in gel form may comprise from 1 to 5% by weight of a viscosifier and most preferably HEC.
- The composition of the invention may be in gel form and may also comprise a humectant such as propylene glycol (PG), glycerol, polyethylene glycol, polydextrose, sorbitol, triethanlolamine, cyclomethicone, ammonium lactate or glycerol ester. Preferably the composition comprises from 5% to 15% by weight of a humectant and most preferably PG.
- Preferably the composition of the invention comprises excipients to optimise binding of the stain to biofilm. For instance, the composition of the invention may also comprise a metal chelating agent such as tetra sodium ethylene diamine tetraacetic acid (EDTA), citric acid, deferasirox, deferiprone, deferoxamine, deferazoxane, ethylene glycol tetraacetic acid, gluonic acid, nitrilotriacetic acid or trisodium citrate at a level of 0.1 to 2.0% by weight, or an agent to assist penetration of the staining agent into the biofilm such as a surfactant for example Tween 80, Span 20, or coamidopropylbetaine (CAPD) and in particular a cationic quaternary ammonium surfactant such as dialkyl dimethyl ammonium chloride, alkyl pyridinium chloride, benzalkonium chloride (BaCl), benzethonium chloride (BeCl), disodium cocamphodiacetate, cetyl morpholinium ethosulphate or an alkyl trimethyl ammonium chloride at a level of 0.1 to 1.0% by weight. The addition of a surfactant may also act as a foaming agent. For example, the surfactants BeCl, Tween 80, Span 20, CAPD, sodium C14-16 olefin sulfonate or Softisan 649 can act as foaming agents to give the formulation characteristics of a foaming mousse.
- Preferably the composition of the invention has a pH in the range of from 5 to 7 and most preferably around 5.5.
- Preferably the composition of the invention is in the form of a viscous fluid and comprises a viscosifier such as HEC, a humectant such as PG, a metal chelator such as EDTA, a surfactant such as BeCl and water and in particular 2.0% w/v hydroxyethylcellulose, 10.0% w/v propylene glycol, 0.5% EDTA, 0.5% BeCl and approximately 87% v/v sterile, distilled water. Alternatively, the compositions of the present invention could be in the form of a solution applied to the wound from a syringe, sachet, spray bottle, aerosol bottle, non-propellant pump bottle, brush or a gel sheet, film or dissolving wafer.
- The formulation could be terminally sterilized by autoclaving or gamma irradiation. Alternatively, the formulation could be a preserved solution containing, for example preservatives such as DMDM hydantoin or parabens such as methyl-, ethyl- or propyl-paraben.
- The composition of the present invention will be used primarily on viable tissues which show signs of clinical infection (inflammation, malodour, purulent exudate, hypoxia, etc.), may be at risk of infection, appear to have slough (host-derived tissue) or biofilm (bacteria-derived tissue) present, or are generally recalcitrant. The composition could also be used at dressing change, in order to detect biofilm, and also to monitor the efficacy of the treatment regime and direct future treatment via the reduction in detected biofilm.
- A further aspect of the present invention relates to a kit of parts for use in the detection of biofilms on viable tissue, the kit comprising:
-
- a composition comprising a staining agent which preferentially stains biofilms and
- a light source capable of causing the staining agent to fluoresce.
- Preferably the kit further comprises optical filters to enable the specific detection of fluorescence in the form of spectacles or integrated optical filters.
- The light source may be a white light source such as tungsten, halogen or pulsed xenon lamp that is passed through a “short pass” filter. Preferably the light source is a monochromatic or narrow spectrum source that does not require filtering or attenuation such as a light emitting diode with an output that closely correlates with the spectral characteristics of the staining agent. As such the light source is preferably small, portable, hand-held and generates no or negligible heat. The light source may be multiple use or fully disposable.
- Preferably the kit further comprises spectacles, or the light source contains an integrated lens, for use in detecting the biofilm wherein the spectacles or lens contain a filter to exclude all wavelengths of light below the absorption maxima (Absmax) of the staining agent. In this way the user is assisted in detecting the fluorescence of the staining agent present in the biofilm. More preferably the spectacles or lens filter is efficient at transmitting wavelengths of light corresponding to the fluorescence emission spectra of the staining agent. The spectacles can be multiple use or fully disposable (in the same way that an integrated lens in the light source could be).
- Preferably the kit further comprises a wound irrigation solution for rinsing the viable tissue before and/or after illumination with the light source.
- In an example of typical use, a composition according to the invention is applied to the whole wound or desired regions in order to achieve a thin but consistent layer of composition for instance 0.1 to 0.5 cm in thickness. The composition is left in place for 0.1 to 15 minutes, more preferably 0.5 to 2 minutes. Prior to any illumination, the formulation can be left in place or more preferably excess formulation is rinsed away from the wound using a suitable wound irrigation solution. Rinsing the excess formulation away can enable the staining to show where the biofilm is in the wound so that these areas can be treated for example by curette.
- The wound is then inspected for presence of preferentially stained biofilm. This may be done with the naked eye or the wound can be illuminated with a light source at a distance in the range of 1 to 50 cm. Preferably the light source is at a distance of 5 to 20 cm. The light source spectral output is selected to correspond to the fluorescence emission spectra of the staining agent; for example, for Rose Bengal, which has a fluorescence maxima of 575 nm, a suitable light source might emit wavelengths of 550-600 nm. This causes the agent in the stained biofilm to fluoresce. Preferably the user wears spectacles to observe the wound or observes the wound through an integrated lens in the light source. The spectacles or lens have a light filtration system which excludes wavelengths of light below the fluorescence emission range of the staining agent and allows any fluorescence to be seen remarkably clearly and specifically and thus, detection of any biofilm present.
- Typically, treatment should take place at subsequent dressing changes. The wound can be further inspected for presence and reduction of biofilm with the naked eye or with the light source and spectacles or integrated lens. The wound can then be dressed with an appropriate primary and secondary dressing.
- The following is a brief description of the figures and tables:
-
FIG. 1 . Initial screening of selected dyes as biofilm stains using biofilms grown in the CDFF (n=6). -
FIG. 2 a. Screening of selected dyes as biofilm stains using biofilms grown in the CDC biofilm reactor (n=9). Absorption data. -
FIG. 2 b. Screening of selected dyes as biofilm stains using biofilms grown in the CDC reactor (n=9). Concentration data. -
FIG. 3 . Rose Bengal with EDTA and BaCl (RBEB) stained (A-C) and control (D-E) samples of meat containing mixed S. aureus and P. aeruginosa biofilms. -
FIG. 4 . S. aureous biofilm age vs dye concentration. -
FIG. 5 . P. aeruginosa biofilm age vs Rose Bengal concentration. - The following examples are illustrative of the present invention.
- A constant depth biofilm fermenter (CDFF) was used to culture 4-day mixed biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. Briefly, biofilms were formed in recesses in 15 PTFE pans around the rim of a rotating steel turntable onto which inoculum or sterile growth media was allowed to steadily drip. A scraper bar distributed bacterial inoculum or media over the pans as the turntable rotated, maintaining the biofilms at a steady depth. Each pan contained 5 removable plugs, 4 mm in diameter which were recessed to a depth of 300 μm. The resulting biofilms that grew in the plug recesses were reproducible in terms of appearance and microbiological composition and could be removed from the CDFF through a sampling port using sterile instruments. In duplicate, biofilm-containing pans were removed from the CDFF, rinsed once by dipping in sterile saline for 5 seconds, then incubated in 10 ml volumes of the following potential biofilm stains at 100 μM concentration in deionised water unless stated, in the dark for two minutes: Erythrosine; Rose Bengal; Fast Green; Rose Bengal with 2% w/v EDTA and 1% w/v benzalkonium chloride (BaCl) (RBEB); Rose Bengal with 2% w/v tetra sodium EDTA and 1% w/v benzalkonium chloride in a 2% w/v hydroxyethyl cellulose and 10% w/v propylene glycol gel (Gel-RBEB). Following another rinse in saline, six plugs for each stain were incubated overnight at 37° C. in 6 ml volumes of 2% sodium dodecyl sulphate (SDS) in water for digestion. Samples were then spun at 13,000 rpm for 10 minutes to separate the supernatant from digested cell debris. Absorption spectra of the aspired supernatants were then measured in a spectrophotometer and these spectra compared to spectra of known concentrations (100 μM) of each stain in 2% SDS.
-
FIG. 1 shows that Fast Green appeared to be the most effective stain of biofilms grown in the CDFF, closely followed by Rose Bengal with EDTA and BaCl in liquid form. The addition of the EDTA and BaCl excipients appeared to enhance the uptake of Rose Bengal into the biofilms. - A Centre for Disease Control (CDC) biofilm reactor was used to culture 48hour mixed biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. Briefly, biofilms were formed on coupons on reactor rods which were held within the reactor vessel containing a continuously mixed culture of S. aureus and P. aeruginosa at 35° C. Biofilm-containing coupons were removed from the rods, rinsed once by dipping in sterile saline for 5 seconds, then incubated in 10 ml volumes of the following biofilm stains at 100 μM concentration in deionised water, in the dark for two minutes: Erythrosine; Rose Bengal; Alcian Blue; Rhodamine B;
Rhodamine 123; Rose Bengal with 2% w/v EDTA and 1% w/v BaCl (RBEB). Following another rinse in saline, nine coupons for each stain were each added to 2 ml volumes of 2% SDS for stomaching at ‘high’ setting for 1 minute then incubated overnight in the dark at 37° C., for digestion. Samples were then spun at 13,000 rpm for 5 minutes to separate the supernatant from digested cell debris. Absorption spectra of the aspired supernatants were then measured in a spectrophotometer and these spectra compared to spectra of known concentrations (100 μM) of each stain in 2% SDS. -
FIG. 2 a shows how in terms of absolute absorption or ‘brightness’, RBEB was the most efficient biofilm stain, followed by the carbohydrate stain, Alcian Blue. The EDTA and/or BaCl appeared to enhance the uptake of Rose Bengal by more than 60%. When the same data was expressed as a ratio of measured absorption: absorption at 100 1.1M (i.e. concentration), Alcian Blue appeared to be the most effective biofilm stain. - A pork belly biofilm model was used to further assess biofilm stains. Pieces of pork belly were cut using a 20 mm bore and a 6 mm borer was used to create indentations in the centre of the samples. Samples were then sterilized by gamma irradiation. Samples were inoculated with 10 μl volumes of a ˜1×107 cfu/ml mixed suspension of S. aureus and P. aeruginosa then incubated at 35° C. in Parafilm-sealed Petri dishes for 72-96 hours. Samples that appeared to have visible biofilms in the central bore hole only were then stained by dipping the samples into 10 ml volumes of 100 μM Rose Bengal with 2% w/v EDTA and 1% w/v BaCl (RBEB) for two minutes with rinsing in saline before and after staining Control samples were not stained and therefore only dipped in saline for 2 minutes. Samples were then photographed, examples of which are shown in
FIG. 3 . The three samples (AtoC) shown that were stained with RBEB clearly demonstrate the selective uptake of the stain by the biofilms which were contained within the indentations. The three control samples show that without this staining it is difficult to ascertain if and where biofilm is present in the samples. - A membrane filter biofilm model was used to study the effect of Rose Bengal concentration on the efficacy of biofilm staining Briefly, a 5 μl volume of a 5×105 cfu/ml suspension of S. aureus or P. aeruginosa was added to the centre of sterile membrane filter discs (pore size 0.2 μm; Anodisc, Whatman) which were placed onto 7 ml volumes of sterile Tryptic Soya Broth in lidded 6-well plates. Samples were incubated for 4 hours (immature biofilms), and 24 and 48 hours (mature biofilms), and excess planktonic cells were rinsed from the filters. The biofilms on the filters were stained by pipetting 2 ml volumes of Rose Bengal (60 μM or 300 μM) or saline (negative control) for 30 seconds, followed by rinsing. Rose Bengal was recovered from the biofilm samples by stomaching and overnight digestion in 2% sodium dodecyl sulphate, centrifugation, then measuring of absorption spectra in a UV-vis spectrophotometer. Rose Bengal uptake per sample was determined by comparison of absorption values with a standard curve of Rose Bengal in 2% SDS. Tables 1 and 2 show that mature S. aureus and P. aeruginosa biofilms took up Rose Bengal in a dye concentration- and biofilm age-dependent manner. Only at the highest concentrations of 300 μM did the immature, 4-hour biofilms appear to be stained, although this was likely due to some staining of the filters themselves.
FIGS. 4 and 5 show how mature, 48-hour biofilms of S. aureus and P. aeruginosa took up more Rose Bengal than 24-hour biofilms, and also that 300 μM Rose Bengal resulted in significantly more staining of the biofilms than 60 μM Rose Bengal. This simple method for quantifying the uptake of Rose Bengal by biofilms utilises the absorption spectra of the dye. Using the absorption spectra to quantify the dye is similar to detecting the fluorescence using a light source with optical filters. Uptake could then alternatively be observed qualitatively using the naked eye or by observing the fluorescence emission of the Rose Bengal in conjunction with a light source and optical filter. -
TABLE 1 Age (h) [RB] (uM) 1 2 3 Avg S.D. 4 0 0 0 0 0 0 4 60 0 0 0 0 0 4 300 1.59 1.45 1.64 1.56 0.10 24 0 0 0 0 0 0 24 60 1.66 1.72 2.17 1.85 0.28 24 300 4.82 4.55 3.95 4.44 0.45 48 0 0 0 0 0 0 48 60 1.22 1.12 1.37 1.24 0.12 48 300 6.26 5.18 5.71 5.72 0.54 -
TABLE 2 Age (h) [RB] (uM) 1 2 3 Avg S.D. 4 0′ 0 0 0 0 0 4 60 0 0 0 0 0 4 300 1.52 1.76 1.88 1.72 0.18 24 0 0 0 0 0 0 24 60 2.04 1.76 2.02 1.94 0.15 24 300 5.0.5 5.84 4.41 5.10 0.72 48 0 0 0 0 0 0 48 60 3.15 1.66 2.37 2.39 0.74 48 300 6.20 9.01 6.69 7.30 1.50
Claims (21)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB1020236.4A GB201020236D0 (en) | 2010-11-30 | 2010-11-30 | A composition for detecting biofilms on viable tissues |
GB1020236.4 | 2010-11-30 | ||
PCT/GB2011/001665 WO2012072980A1 (en) | 2010-11-30 | 2011-11-30 | A composition for detecting biofilms on viable tissues |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2011/001665 A-371-Of-International WO2012072980A1 (en) | 2010-11-30 | 2011-11-30 | A composition for detecting biofilms on viable tissues |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US16/295,774 Continuation US11135315B2 (en) | 2010-11-30 | 2019-03-07 | Composition for detecting biofilms on viable tissues |
Publications (1)
Publication Number | Publication Date |
---|---|
US20140161728A1 true US20140161728A1 (en) | 2014-06-12 |
Family
ID=43500822
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US13/990,755 Abandoned US20140161728A1 (en) | 2010-11-30 | 2011-11-30 | Composition for detecting biofilms on viable tissues |
US16/295,774 Active US11135315B2 (en) | 2010-11-30 | 2019-03-07 | Composition for detecting biofilms on viable tissues |
US17/398,560 Abandoned US20210369874A1 (en) | 2010-11-30 | 2021-08-10 | Composition for detecting biofilms on viable tissues |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US16/295,774 Active US11135315B2 (en) | 2010-11-30 | 2019-03-07 | Composition for detecting biofilms on viable tissues |
US17/398,560 Abandoned US20210369874A1 (en) | 2010-11-30 | 2021-08-10 | Composition for detecting biofilms on viable tissues |
Country Status (20)
Country | Link |
---|---|
US (3) | US20140161728A1 (en) |
EP (1) | EP2646566B1 (en) |
JP (3) | JP2014500495A (en) |
CN (2) | CN108330157A (en) |
AU (2) | AU2011334682B2 (en) |
BR (1) | BR112013013662A2 (en) |
CA (1) | CA2819303C (en) |
DK (1) | DK2646566T3 (en) |
ES (1) | ES2609612T3 (en) |
GB (1) | GB201020236D0 (en) |
HK (1) | HK1258842A1 (en) |
HU (1) | HUE032884T2 (en) |
LT (1) | LT2646566T (en) |
MX (1) | MX344623B (en) |
NZ (1) | NZ612573A (en) |
PL (1) | PL2646566T3 (en) |
PT (1) | PT2646566T (en) |
RU (1) | RU2650803C2 (en) |
SI (1) | SI2646566T1 (en) |
WO (1) | WO2012072980A1 (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20170315120A1 (en) * | 2016-05-02 | 2017-11-02 | James M. Rynerson | Methods and devices for detecting biofilms on the eyelid margins |
WO2018157368A1 (en) * | 2017-03-03 | 2018-09-07 | The Procter & Gamble Company | Calcium fluorescent probes to assess oral care composition efficacy in biofilm |
US11135315B2 (en) | 2010-11-30 | 2021-10-05 | Convatec Technologies Inc. | Composition for detecting biofilms on viable tissues |
US11452291B2 (en) | 2007-05-14 | 2022-09-27 | The Research Foundation for the State University | Induction of a physiological dispersion response in bacterial cells in a biofilm |
US11541105B2 (en) | 2018-06-01 | 2023-01-03 | The Research Foundation For The State University Of New York | Compositions and methods for disrupting biofilm formation and maintenance |
US11572578B2 (en) * | 2018-07-31 | 2023-02-07 | Saraya Co., Ltd. | Reagent kit for detecting biofilm and method for detecting biofilm |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0525504D0 (en) | 2005-12-14 | 2006-01-25 | Bristol Myers Squibb Co | Antimicrobial composition |
US20130281913A1 (en) | 2012-04-20 | 2013-10-24 | Klox Technologies Inc. | Biophotonic compositions and methods for providing biophotonic treatment |
US11116841B2 (en) | 2012-04-20 | 2021-09-14 | Klox Technologies Inc. | Biophotonic compositions, kits and methods |
SE536793C2 (en) * | 2012-07-02 | 2014-08-19 | Richter Life Science Dev Ab | Carbohydrate detection |
CA2895896A1 (en) | 2012-12-20 | 2014-06-26 | Convatec Technologies Inc. | Processing of chemically modified cellulosic fibres |
US20140276354A1 (en) | 2013-03-14 | 2014-09-18 | Klox Technologies Inc. | Biophotonic materials and uses thereof |
CA2916337C (en) | 2013-07-03 | 2022-03-22 | Klox Technologies Inc. | Biophotonic compositions comprising a chromophore and a gelling agent for treating wounds |
RU2553431C2 (en) * | 2013-07-09 | 2015-06-10 | Государственное бюджетное учреждение здравоохранения города Москвы Научно-исследовательский институт скорой помощи имени Н.В. Склифосовского Департамента здравоохранения г. Москвы | Test system for human interferon activity testing |
US10465038B2 (en) * | 2013-12-26 | 2019-11-05 | Daicel Corporation | Curable composition for lens, lens and optical device |
CN103694732A (en) * | 2013-12-31 | 2014-04-02 | 江苏省原子医学研究所 | Hematoxylin staining solution and HE (hematoxylin eosin) staining solution containing hematoxylin staining solution |
WO2015149177A1 (en) | 2014-04-01 | 2015-10-08 | Klox Technologies Inc. | Tissue filler compositions and methods of use |
ES2856841T3 (en) | 2014-10-31 | 2021-09-28 | Klox Tech Inc | Photoactivatable fibers and fabric media |
KR101688212B1 (en) * | 2014-12-15 | 2016-12-20 | 한국화학연구원 | Process for preparing photonic crystal polymer substrate for florescence signal amplication |
US10416153B2 (en) | 2015-04-24 | 2019-09-17 | Nanyang Technological University | Chemical fluorescent probes for detecting biofilms |
KR20170099739A (en) | 2016-02-23 | 2017-09-01 | 노을 주식회사 | Contact-type staining-assist patch, manufacturing method thereof and staining method using the patch |
US10371610B2 (en) | 2016-02-23 | 2019-08-06 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
DE102016113166B4 (en) * | 2016-07-18 | 2022-02-24 | Joanneum Research Forschungsgesellschaft Mbh | Method for detecting a biofilm and means for detecting a biofilm |
JP7511585B2 (en) | 2019-06-03 | 2024-07-05 | コンバテック リミティド | Methods and devices for destroying and containing pathogens - Patents.com |
RU2718910C1 (en) * | 2020-01-28 | 2020-04-15 | Общество с ограниченной ответственностью «БФР лабораториз» | Indicator for detecting biological films of bacteria on medical instruments (versions) |
US20230078234A1 (en) * | 2020-02-26 | 2023-03-16 | Meiji Pharmaceutical University | Method for evaluating biofilm formation, and invertebrate for use in evaluating biofilm formation |
IT202200000689A1 (en) * | 2022-01-18 | 2023-07-18 | Marco Balato | USE OF DYES IN THE SELECTIVE IDENTIFICATION OF INFECTED SITES IN BIOFILM-RELATED INFECTIONS |
WO2024024648A1 (en) * | 2022-07-29 | 2024-02-01 | パナソニックIpマネジメント株式会社 | Organic pigment composition, and spray agent comprising same |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6555508B1 (en) * | 2000-02-17 | 2003-04-29 | Leonard Paul | Liquid foaming soap compositions |
US20080226724A1 (en) * | 2007-01-19 | 2008-09-18 | Genentech, Inc. | Prevention of hydrogel viscosity loss |
WO2010070292A1 (en) * | 2008-12-20 | 2010-06-24 | Convatec Technologies Inc | A composition for use on skin and wound |
Family Cites Families (745)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2396514A (en) | 1943-03-20 | 1946-03-12 | Ludwig Jekels | Sterilizing materials and methods for making the same |
GB735462A (en) | 1951-12-20 | 1955-08-24 | Dow Chemical Co | Improvements in or relating to iodine-containing ethylenediaminetetraacetic acid compounds and methods of making same |
US2785106A (en) | 1952-08-16 | 1957-03-12 | Ions Exchange And Chemical Cor | Process for making antiseptic article |
US3092552A (en) | 1958-05-19 | 1963-06-04 | Albert C Nolte | Oligodynamic silver compositions and uses |
US3061469A (en) | 1959-11-05 | 1962-10-30 | Givaudan Corp | Antibacterial textile fabrics |
US3246624A (en) | 1965-02-05 | 1966-04-19 | Novo Ind Corp | Pressure indicating device |
JPS5738564B2 (en) * | 1974-09-27 | 1982-08-16 | ||
US4258056A (en) | 1978-12-18 | 1981-03-24 | Economics Laboratory, Inc. | Control of mastitis and compositions therefor |
JPS6015641B2 (en) | 1981-03-18 | 1985-04-20 | 花王株式会社 | Method for producing fibrous carboxymethyl cellulose |
EP0124536A4 (en) | 1982-11-05 | 1985-06-06 | Baxter Travenol Lab | Antimicrobial compositions. |
US4655758A (en) | 1982-12-16 | 1987-04-07 | Johnson & Johnson Products, Inc. | Microbial polysaccharide articles and methods of production |
US4889654A (en) | 1984-07-31 | 1989-12-26 | Rio Linda Chemical Company, Inc. | Aqueous foam disinfectant containing chlorine dixoide and preparation and use thereof |
US4612337A (en) | 1985-05-30 | 1986-09-16 | The Trustees Of Columbia University In The City Of New York | Method for preparing infection-resistant materials |
US4728323A (en) | 1986-07-24 | 1988-03-01 | Minnesota Mining And Manufacturing Company | Antimicrobial wound dressings |
FR2604900B1 (en) | 1986-10-08 | 1989-01-13 | Blomet Marie Claude | PHYSIOLOGICAL SOLUTION FOR THE WASHING OF THE PARTS OF THE HUMAN BODY WHICH HAVE BEEN IN CONTACT WITH A FLUORHYDRIC ACID AND CONCENTRATED FOR ITS PREPARATION |
GB8709498D0 (en) | 1987-04-22 | 1987-05-28 | Bay M | Wound dressing |
US4829129A (en) | 1987-05-29 | 1989-05-09 | International Dioxcide, Inc. | Reaction product of polymer with chlorine dioxide |
GB2220881B (en) | 1988-04-28 | 1992-07-08 | Toyo Boseki | Improvements in or relating to superabsorbent materials |
US4906100A (en) | 1988-05-13 | 1990-03-06 | University Of Cincinnati | Method of detecting adriamycin (doxorubicin) or daunomycin in the environment |
RU2092180C1 (en) | 1988-06-22 | 1997-10-10 | Эпплаид Микробиолоджи Инк. | Antibacterial composition |
US4973848A (en) | 1989-07-28 | 1990-11-27 | J. Mccaughan | Laser apparatus for concurrent analysis and treatment |
US5340924A (en) | 1990-01-18 | 1994-08-23 | Morinaga Milk Industry Co., Ltd. | Method for heat treatment of lactoferrin without losing physiological activities thereof |
US6066469A (en) | 1990-03-08 | 2000-05-23 | Ferro Dynamics, Inc. | Cloning, expression, and uses of human lactoferrin |
JPH04282311A (en) | 1991-03-08 | 1992-10-07 | Koike Kagaku Kk | Aerosol-type foamable wound-disinfectant |
WO1992018098A1 (en) | 1991-04-10 | 1992-10-29 | Capelli Christopher C | Antimicrobial compositions useful for medical applications |
US5662913A (en) | 1991-04-10 | 1997-09-02 | Capelli; Christopher C. | Antimicrobial compositions useful for medical applications |
GB9126193D0 (en) | 1991-12-10 | 1992-02-12 | Courtaulds Plc | Cellulosic fibres |
US5407656A (en) | 1992-03-04 | 1995-04-18 | Arco Research Co., Inc. | Method and compositions for the production of chlorine dioxide |
DE69311706T2 (en) | 1992-03-20 | 1997-12-18 | Unilever Nv | IMPROVEMENT IN OR WITH REGARD TO CLEANING AGENTS |
US5762620A (en) | 1992-04-02 | 1998-06-09 | Ndm Acquisition Corp. | Wound dressing containing a partially dehydrated hydrogel |
HUT70688A (en) | 1992-07-22 | 1995-10-30 | Unilever Nv | Process for combating of surface bound micro-organisms |
EP0665733B1 (en) | 1992-10-21 | 2003-05-07 | Gynetech Laboratories, Inc. | Vaginal sponge delivery system |
GB9301258D0 (en) | 1993-01-22 | 1993-03-17 | Courtaulds Plc | Use of absorbent fibre |
WO1994016746A1 (en) | 1993-01-22 | 1994-08-04 | Courtaulds Plc | Wound dressings |
US5848995A (en) | 1993-04-09 | 1998-12-15 | Walder; Anthony J. | Anti-infective medical article and method for its preparation |
US5567495A (en) | 1993-08-06 | 1996-10-22 | The Trustees Of Columbia University In The City Of New York | Infection resistant medical devices |
US5744155A (en) | 1993-08-13 | 1998-04-28 | Friedman; Doron | Bioadhesive emulsion preparations for enhanced drug delivery |
JP2746527B2 (en) * | 1993-09-14 | 1998-05-06 | 鐘紡株式会社 | Slow-dyeing hair dye composition and method for producing the same |
US5670169A (en) * | 1993-12-20 | 1997-09-23 | E.R. Squibb & Sons, Inc. | Wound hydrating gel with novel preservative system and low cytotoxicity |
JP3121503B2 (en) | 1994-10-18 | 2001-01-09 | レンゴー株式会社 | Antibacterial agent |
JP3297969B2 (en) | 1994-12-26 | 2002-07-02 | ライオン株式会社 | Eye drops |
US5616347A (en) | 1995-02-14 | 1997-04-01 | Alliger; Howard | Chlorine dioxide skin medicating compositions for preventing irritation |
SE506869C2 (en) | 1995-06-30 | 1998-02-23 | Inst Polymerutveckling Ab | Method of lamination by photo-grafting, laminates made according to the method and its use |
CA2225808C (en) | 1995-06-30 | 2002-12-17 | Christopher C. Capelli | Silver-based pharmaceutical compositions |
DE19609143C1 (en) | 1996-03-08 | 1997-11-13 | Rhodia Ag Rhone Poulenc | Melt-blown fleece, process for its production and its uses |
US5820918A (en) | 1996-07-11 | 1998-10-13 | Hercules Incorporated | Medical devices containing in-situ generated medical compounds |
EP0920252A4 (en) | 1996-08-16 | 2004-08-18 | Christopher C Capelli | Silver-based antimicrobial compositions |
US20030104018A1 (en) | 1996-12-31 | 2003-06-05 | Griscom Bettle | Skin product having micro-spheres, and processes for the production thereof |
EP0925396B1 (en) | 1997-04-11 | 2003-05-02 | Bristol-Myers Squibb Company | Knitted wound dressings |
KR20010013377A (en) | 1997-06-04 | 2001-02-26 | 데이비드 엠 모이어 | Mild, leave-on antimicrobial compositions |
US5993864A (en) | 1997-07-11 | 1999-11-30 | Kross; Robert D. | Chlorine dioxide chelate compositions and method of use |
US5860947A (en) | 1997-08-20 | 1999-01-19 | Stamler; Keith D. | Wound irrigation device and method |
US6605751B1 (en) | 1997-11-14 | 2003-08-12 | Acrymed | Silver-containing compositions, devices and methods for making |
US6753063B1 (en) | 1997-11-19 | 2004-06-22 | The Procter & Gamble Company | Personal cleansing wipe articles having superior softness |
IL123437A0 (en) | 1998-02-24 | 1998-09-24 | Shalev Pinchas | Apparatus and method for photothermal destruction of oral bacteria |
JP2002519153A (en) | 1998-07-01 | 2002-07-02 | アコーディス スペシャリティー ファイバーズ リミティド | Manufacture of wound dressings, their manufacture and materials suitable for their use |
US6413556B1 (en) | 1999-01-08 | 2002-07-02 | Sky High, Llc | Aqueous anti-apoptotic compositions |
DE19909214A1 (en) | 1999-03-03 | 2000-09-07 | Basf Ag | Water-absorbent, foam-like, crosslinked polymers with improved distribution effect, process for their preparation and their use |
GB9905663D0 (en) | 1999-03-12 | 1999-05-05 | Bristol Myers Squibb Co | Iodine preparation composition |
ES2270824T3 (en) | 1999-04-02 | 2007-04-16 | Kci Licensing, Inc. | CLOSURE SYSTEM ASSISTED BY VACUUM WITH HEATING AND COOLING PROVISION. |
US6172040B1 (en) | 1999-05-28 | 2001-01-09 | A. Satyanarayan Naidu | Immobilized lactoferrin antimicrobial agents and the use thereof |
EP1216319B1 (en) | 1999-09-27 | 2004-04-07 | BHK Holding Ltd. | Absorptive fabric |
EP1216065B1 (en) | 1999-10-01 | 2008-10-29 | Acrymed | Silver-containing compositions, devices and methods for making |
US6592890B1 (en) | 1999-10-20 | 2003-07-15 | Oxibio, Inc. | Conveyance of anti-infective activity to wound dressings |
AU2001239835A1 (en) | 2000-02-26 | 2001-09-03 | Advanced Photodynamic Technologies, Inc. | Photodynamic cellular and acellular organism eradication utilizing a photosensitive material and surfactant |
GB0011202D0 (en) | 2000-05-09 | 2000-06-28 | Kci Licensing Inc | Abdominal wound dressing |
US8283135B2 (en) | 2000-06-30 | 2012-10-09 | The Procter & Gamble Company | Oral care compositions containing combinations of anti-bacterial and host-response modulating agents |
CA2386985A1 (en) | 2000-08-11 | 2002-02-21 | The Lawson Health Research Institute | Compositions and methods for inhibiting islet dysfunction and autoimmune disorders |
US20020091074A1 (en) | 2000-09-20 | 2002-07-11 | Wooley Richard E. | Medical compositions, dressings and methods for treating microbial infections of skin lesions |
US20070003508A1 (en) | 2005-07-01 | 2007-01-04 | Wooley Richard E | Methods and compositions for promoting wound healing |
GB2370226A (en) | 2000-09-21 | 2002-06-26 | Acordis Speciality Fibres Ltd | Wound dressing |
BRPI0115730B8 (en) | 2000-11-29 | 2021-07-27 | Bristol Myers Squibb Co | method of preparing a material comprising a hydrophilic, amphoteric or anionic polymer that has antimicrobial activity |
US7700819B2 (en) | 2001-02-16 | 2010-04-20 | Kci Licensing, Inc. | Biocompatible wound dressing |
US20020172709A1 (en) | 2001-03-30 | 2002-11-21 | Brian Nielsen | Medical dressing comprising an antimicrobial silver compound and a method for enhancing wound healing |
JP4517101B2 (en) * | 2001-08-21 | 2010-08-04 | 学校法人日本大学 | Plaque staining composition |
AU2002334063B2 (en) | 2001-09-12 | 2008-08-14 | Acordis Speciality Fibres Limited | Antibacterial wound dressing |
GB2382527A (en) | 2001-12-03 | 2003-06-04 | Acordis Speciality Fibres Ltd | Wound dressings |
US8541472B2 (en) | 2001-12-05 | 2013-09-24 | Aseptica, Inc. | Antiseptic compositions, methods and systems |
JP4165754B2 (en) | 2001-12-05 | 2008-10-15 | タイコ・ヘルスケアー・グループ・エルピー | Antimicrobial system and method |
CZ14554U1 (en) | 2002-02-11 | 2004-07-26 | Unimed Pharma Spol. S R. O. | Eyewater based on iodide anion |
US6468965B1 (en) | 2002-04-22 | 2002-10-22 | Paul Cutler | Pharmaceutical composition for oral administration of a mixture of chelating agents |
US20040033916A1 (en) | 2002-06-28 | 2004-02-19 | Kuzmin Vladimir Semenovich | Disinfecting composition |
GB0215023D0 (en) | 2002-06-28 | 2002-08-07 | Bristol Myers Squibb Co | Wound dressing |
GB2393120A (en) | 2002-09-18 | 2004-03-24 | Johnson & Johnson Medical Ltd | Compositions for wound treatment |
CN1688322B (en) | 2002-09-25 | 2012-08-15 | 埃德温·奥德尔·迈纳 | Antiseptic solutions containing silver chelated with polypectate and EDTA |
DE10393374D2 (en) | 2002-10-14 | 2005-09-01 | Arconia Gmbh Malans | composition |
GB0224986D0 (en) | 2002-10-28 | 2002-12-04 | Smith & Nephew | Apparatus |
WO2004056346A1 (en) | 2002-12-19 | 2004-07-08 | University Of Georgia Research Foundation, Inc. | Methods and compositions for wound management |
CN1771061B (en) | 2003-01-09 | 2010-05-12 | 聚合物器官股份有限公司 | Biomedical foams |
US7629000B2 (en) | 2003-05-13 | 2009-12-08 | E.I. Du Pont De Nemours And Company | Method for making antimicrobial polyester-containing articles with improved wash durability and articles made thereby |
JP2007501683A (en) * | 2003-05-22 | 2007-02-01 | エラン ファーマ インターナショナル リミテッド | Sterilization of nanoparticle active substance dispersions by gamma irradiation |
CN102743785A (en) | 2003-08-14 | 2012-10-24 | 美利肯公司 | Silver-containing wound care device, composition therefor, and method of producing |
US20050202066A1 (en) | 2003-08-28 | 2005-09-15 | Arata Andrew B. | Silver dihydrogen citrate compositions comprising a second antimicrobial agent |
US20050059731A1 (en) | 2003-09-16 | 2005-03-17 | Ceramoptec Industries, Inc. | Erythrosin-based antimicrobial photodynamic therapy compound and its use |
GB0325129D0 (en) | 2003-10-28 | 2003-12-03 | Smith & Nephew | Apparatus in situ |
FI117056B (en) * | 2003-11-06 | 2006-05-31 | Kemira Oyj | Procedure for monitoring the presence of biofilm forming microorganisms in the paper industry |
US7033511B2 (en) | 2004-01-20 | 2006-04-25 | A-Dec, Inc. | Sustained water treatment in dental equipment |
JP2005210997A (en) * | 2004-01-30 | 2005-08-11 | Nichirei Corp | Stain of biofilm |
WO2005079582A1 (en) | 2004-02-24 | 2005-09-01 | Campina B.V. | Antimicrobial lactoferrin compositions for surfaces, cavities, and foodstuff |
US7909805B2 (en) | 2004-04-05 | 2011-03-22 | Bluesky Medical Group Incorporated | Flexible reduced pressure treatment appliance |
US20070254349A1 (en) | 2004-04-16 | 2007-11-01 | Helbo Photodynamic Systems Gmbh & Co.Kg | Preparation for the Photodynamic Control of Micro-Organisms and Use Thereof |
US10413644B2 (en) | 2004-04-27 | 2019-09-17 | Smith & Nephew Plc | Wound treatment apparatus and method |
GB0409446D0 (en) | 2004-04-28 | 2004-06-02 | Smith & Nephew | Apparatus |
RU2007101243A (en) | 2004-06-12 | 2008-07-20 | Сигнум Байосайенсиз | COMPOSITIONS AND METHODS FOR LOCAL APPLICATION FOR CONDITIONS RELATED TO EPITEL |
GB2415372A (en) | 2004-06-23 | 2005-12-28 | Destiny Pharma Ltd | Non photodynamical or sonodynamical antimicrobial use of porphyrins and azaporphyrins containing at least one cationic-nitrogen-containing substituent |
ATE410535T1 (en) | 2004-07-09 | 2008-10-15 | Johnson & Johnson Gmbh | COSMETIC AND/OR DERMATOLOGICAL ABSORBENT PERSONAL CARE ARTICLE HAVING AT LEAST ONE ABSORBENT LAYER |
CA2573744A1 (en) | 2004-07-22 | 2006-03-02 | Ondine International Ltd. | Sonophotodynamic therapy for dental applications |
EP1781098B1 (en) | 2004-07-30 | 2015-10-07 | Avent, Inc. | Antimicrobial devices and compositions |
US20060115440A1 (en) | 2004-09-07 | 2006-06-01 | Arata Andrew B | Silver dihydrogen citrate compositions |
US20060051430A1 (en) | 2004-09-07 | 2006-03-09 | Arata Andrew B | Silver dihydrogen citrate compositions |
US9028852B2 (en) | 2004-09-07 | 2015-05-12 | 3M Innovative Properties Company | Cationic antiseptic compositions and methods of use |
GB0424833D0 (en) | 2004-11-10 | 2004-12-15 | Photocure Asa | Method |
CA2593482A1 (en) | 2005-01-14 | 2006-07-20 | Neurogen Corporation | Heteroaryl substituted quinolin-4-ylamine analogues |
US20060234959A1 (en) | 2005-04-14 | 2006-10-19 | Advanced Photodynamic Technologies, Inc. | Photodynamic therapy utilizing multiple duty cycle light modulation |
FR2884422B1 (en) | 2005-04-18 | 2008-04-11 | Roquette Freres | ANTI-INFLAMMATORY COMPOSITION OF INTESTINES COMPRISING BRANCHED MALTODEXTRINS |
US9162005B2 (en) | 2005-04-25 | 2015-10-20 | Arch Biosurgery, Inc. | Compositions for prevention of adhesions and other barrier applications |
US20060254988A1 (en) | 2005-05-10 | 2006-11-16 | Confirm Monitoring Systems, Inc. | Methods and compositions for the treatment of water |
EP2351828B1 (en) * | 2005-05-24 | 2017-10-18 | NewSouth Innovations Pty Limited | Methods and compositions for regulating biofilm development |
GB0513552D0 (en) | 2005-07-01 | 2005-08-10 | Bristol Myers Squibb Co | Bandage |
DK1922044T3 (en) | 2005-09-07 | 2015-05-26 | Smith & Nephew Inc | CLOSED wound dressing |
AU2006287460A1 (en) | 2005-09-07 | 2007-03-15 | Tyco Healthcare Group Lp | Wound dressing with vacuum reservoir |
GB0525504D0 (en) | 2005-12-14 | 2006-01-25 | Bristol Myers Squibb Co | Antimicrobial composition |
JP2007167266A (en) * | 2005-12-21 | 2007-07-05 | Lion Corp | Dental plaque detection system and method |
US20070166399A1 (en) | 2006-01-13 | 2007-07-19 | 3M Innovative Properties Company | Silver-containing antimicrobial articles and methods of manufacture |
BRPI0706847A2 (en) | 2006-01-23 | 2011-04-12 | Kci Licensing Inc | wound treatment system, wound treatment system for surgically cleaning and healing a wound site, ultrasonic wound treatment system for treating a wound site, method for treating a wound, multipurpose wound bandage and multipurpose wound dressing bandage |
BRPI0706893B8 (en) | 2006-02-06 | 2021-06-22 | Kci Licensing Inc | rotary reduced pressure adapter to connect a reduced pressure source to a porous pad. |
CA2646195C (en) | 2006-04-11 | 2014-07-08 | Tyco Healthcare Group Lp | Wound dressings with anti-microbial and chelating agents |
US7779625B2 (en) | 2006-05-11 | 2010-08-24 | Kalypto Medical, Inc. | Device and method for wound therapy |
GB2439928A (en) | 2006-07-13 | 2008-01-16 | Ethicon Inc | Hydrogel wound dressings exhibiting reduced fiber losses |
JP4826382B2 (en) | 2006-08-08 | 2011-11-30 | アルケア株式会社 | Antibacterial cellulose fiber and textile product for skin contact |
US7858106B2 (en) | 2006-09-21 | 2010-12-28 | Alcare Co., Ltd. | Antimicrobial fiber and its production method, and antimicrobial fiber product comprising the antimicrobial fiber, its production method and regeneration method |
CA2776011C (en) | 2006-10-13 | 2017-01-10 | Kci Licensing, Inc. | Reduced pressure delivery system having a manually-activated pump for providing treatment to low-severity wounds |
JP4806330B2 (en) * | 2006-10-30 | 2011-11-02 | シスメックス株式会社 | Reticulocyte and platelet measuring reagent, reticulocyte and platelet measuring reagent kit, and reticulocyte and platelet measuring method |
US9034352B2 (en) | 2006-11-14 | 2015-05-19 | Rohm And Haas Company | Microbicide combinations containing silver |
FR2916356B1 (en) | 2007-05-25 | 2009-08-28 | Urgo Soc Par Actions Simplifie | NOVEL AGENT FOR RELOCATING ACTIVE INGREDIENTS IN DRESSINGS CONTAINING AT LEAST ONE FATTY BODY |
GB0710846D0 (en) | 2007-06-06 | 2007-07-18 | Bristol Myers Squibb Co | A wound dressing |
WO2009020612A1 (en) | 2007-08-06 | 2009-02-12 | Stb Lifesaving Technologies, Inc. | Methods and dressing for sealing internal injuries |
GB2452720A (en) | 2007-09-11 | 2009-03-18 | Ethicon Inc | Wound dressing with an antimicrobial absorbent layer and an apertured cover sheet |
JP5511670B2 (en) | 2007-10-11 | 2014-06-04 | スピレイカー・インコーポレイテッド | Sutured incision negative pressure wound treatment apparatus and method of use |
US8808259B2 (en) | 2007-11-21 | 2014-08-19 | T.J. Smith & Nephew Limited | Suction device and dressing |
EP3360519B1 (en) | 2007-11-21 | 2020-11-18 | Smith & Nephew plc | Wound dressing |
EP2231734B1 (en) | 2007-12-12 | 2013-11-06 | 3M Innovative Properties Company | Methods of making an article and articles |
US8152785B2 (en) | 2008-03-13 | 2012-04-10 | Tyco Healthcare Group Lp | Vacuum port for vacuum wound therapy |
US20090234306A1 (en) | 2008-03-13 | 2009-09-17 | Tyco Healthcare Group Lp | Vacuum wound therapy wound dressing with variable performance zones |
KR100868574B1 (en) * | 2008-03-17 | 2008-11-13 | 한국광해관리공단 | Assessing biofilm formation onto natural soil particles |
GB0805162D0 (en) | 2008-03-19 | 2008-04-23 | Bristol Myers Squibb Co | Antibacterial wound dressing |
US20110112492A1 (en) | 2008-04-04 | 2011-05-12 | Vivek Bharti | Wound dressing with micropump |
US20100015245A1 (en) | 2008-04-24 | 2010-01-21 | Joe Harrison | Combination of Copper Cations with Peroxides or Quaternary Ammonium Compounds for the Treatment of Biofilms |
GB0808376D0 (en) | 2008-05-08 | 2008-06-18 | Bristol Myers Squibb Co | Wound dressing |
ES2715633T3 (en) | 2008-05-20 | 2019-06-05 | Univ Health Network | Device and method for imaging and fluorescence monitoring |
US20110130712A1 (en) | 2008-05-21 | 2011-06-02 | Moris Topaz | Wound healing device |
ITAR20080022A1 (en) | 2008-05-26 | 2009-11-27 | Daniele Guidi | DRAINAGE DEVICE, IN PARTICULAR FOR ASPIRATION IN CASE OF SUCTION THERAPIES, FISTULAS, SURGICAL WOUND DEFICIENCIES, DECUBITUS INJURIES, TRAUMAS AND SIMILAR INJURIES. |
EP2666488B1 (en) | 2008-05-30 | 2015-10-07 | KCI Licensing, Inc. | Anisotropic drapes |
US20200113741A1 (en) | 2008-05-30 | 2020-04-16 | Kci Licensing, Inc. | Dressing with tissue viewing capability |
KR101170266B1 (en) | 2008-05-30 | 2012-08-01 | 케이씨아이 라이센싱 인코포레이티드 | Reduced-pressure, linear wound closing bolsters and systems |
US8460698B2 (en) | 2008-08-01 | 2013-06-11 | Milliken & Company | Composite article suitable for use as a wound dressing |
EP2309961B1 (en) | 2008-08-08 | 2017-12-06 | Smith & Nephew, Inc. | Wound dressing of continuous fibers |
GB2463523B (en) | 2008-09-17 | 2013-05-01 | Medtrade Products Ltd | Wound care device |
GB0904582D0 (en) | 2008-09-24 | 2009-04-29 | Lumina Adhesives | Switchable adhesives |
GB0817796D0 (en) | 2008-09-29 | 2008-11-05 | Convatec Inc | wound dressing |
AU2009299453B2 (en) | 2008-10-02 | 2014-10-02 | L.R.R.& D. Ltd. | Interface layer wound dressing |
RU2011114000A (en) | 2008-11-07 | 2012-12-20 | КейСиАй ЛАЙСЕНЗИНГ, ИНК. | BANDS AND SYSTEMS OF Wounds THERAPY WITH REDUCED PRESSURE |
JP5778035B2 (en) | 2008-11-14 | 2015-09-16 | ケーシーアイ ライセンシング インコーポレイテッド | Fluid sac, system, and method for accumulating fluid from a tissue site |
JP5620917B2 (en) | 2008-11-18 | 2014-11-05 | ケーシーアイライセンシング インコーポレイテッド | Composite manifold for decompression |
EP2821095B1 (en) | 2008-11-25 | 2017-08-09 | KCI Licensing, Inc. | Device for delivery of reduced pressure to body surfaces |
US9221963B2 (en) | 2008-11-27 | 2015-12-29 | Speciality Fibres And Materials Ltd. | Absorbent material |
JP5513776B2 (en) | 2008-12-01 | 2014-06-04 | 花王株式会社 | Biofilm remover composition |
WO2010080907A1 (en) | 2009-01-07 | 2010-07-15 | Spiracur Inc. | Reduced pressure therapy of the sacral region |
US8162907B2 (en) | 2009-01-20 | 2012-04-24 | Tyco Healthcare Group Lp | Method and apparatus for bridging from a dressing in negative pressure wound therapy |
GB0902368D0 (en) | 2009-02-13 | 2009-04-01 | Smith & Nephew | Wound packing |
US20190298578A1 (en) | 2009-03-26 | 2019-10-03 | Stephen Shulman | Vented emergency wound dressings with anti-thrombogenic layers |
US10792404B2 (en) | 2009-04-10 | 2020-10-06 | Kci Licensing, Inc. | Methods and devices for applying closed incision negative pressure wound therapy |
GB2470040A (en) | 2009-05-06 | 2010-11-10 | Systagenix Wound Man Ip Co Bv | Wound dressing material comprising N-acetyl cysteine |
US20100310845A1 (en) | 2009-06-03 | 2010-12-09 | Eric Bryan Bond | Fluid permeable structured fibrous web |
AU2010262058B2 (en) | 2009-06-16 | 2013-08-29 | Baxter Healthcare S.A. | Hemostatic sponge |
CN104095709B (en) | 2009-06-16 | 2017-06-13 | 3M创新有限公司 | Conformable Medical with self-supporting substrate |
US20100324516A1 (en) | 2009-06-18 | 2010-12-23 | Tyco Healthcare Group Lp | Apparatus for Vacuum Bridging and/or Exudate Collection |
US8469936B2 (en) | 2009-07-15 | 2013-06-25 | Kci Licensing, Inc. | Reduced-pressure dressings, systems, and methods employing desolidifying barrier layers |
US20110066123A1 (en) | 2009-09-15 | 2011-03-17 | Aidan Marcus Tout | Medical dressings, systems, and methods employing sealants |
GB2474694B (en) | 2009-10-23 | 2011-11-02 | Innovia Films Ltd | Biodegradable composites |
CN102686249B (en) | 2009-11-09 | 2014-06-25 | 3M创新有限公司 | Medical articles and methods of making using immiscible material |
WO2011057175A1 (en) | 2009-11-09 | 2011-05-12 | 3M Innovative Properties Company | Medical articles and methods of making using miscible composition |
GB0919659D0 (en) | 2009-11-10 | 2009-12-23 | Convatec Technologies Inc | A component for a wound dressing |
CN102770165B (en) | 2009-12-22 | 2016-07-06 | 史密夫和内修有限公司 | Equipment for negative pressure wound therapy |
EP2525955B1 (en) | 2010-01-20 | 2019-06-12 | KCI Licensing, Inc. | Foam wound inserts with regions of higher and lower densities, wound dressings, and methods |
US8791315B2 (en) | 2010-02-26 | 2014-07-29 | Smith & Nephew, Inc. | Systems and methods for using negative pressure wound therapy to manage open abdominal wounds |
US10709883B2 (en) | 2010-03-04 | 2020-07-14 | Donald Spector | Bandage with microneedles for antimicrobial delivery and fluid absorption from a wound |
US8721606B2 (en) | 2010-03-11 | 2014-05-13 | Kci Licensing, Inc. | Dressings, systems, and methods for treating a tissue site |
US8469935B2 (en) | 2010-03-11 | 2013-06-25 | Kci Licensing, Inc. | Abdominal treatment systems, delivery devices, and methods |
US8430867B2 (en) | 2010-03-12 | 2013-04-30 | Kci Licensing, Inc. | Reduced-pressure dressing connection pads, systems, and methods |
US8814842B2 (en) | 2010-03-16 | 2014-08-26 | Kci Licensing, Inc. | Delivery-and-fluid-storage bridges for use with reduced-pressure systems |
US9358158B2 (en) | 2010-03-16 | 2016-06-07 | Kci Licensing, Inc. | Patterned neo-epithelialization dressings, systems, and methods |
US20110237994A1 (en) | 2010-03-25 | 2011-09-29 | Combat Medical Systems, Llc | Void-filling wound dressing |
WO2011121394A1 (en) | 2010-03-31 | 2011-10-06 | Pharmaplast Sae | A wound care dressing, a method and a production line for manufacturing the wound care dressing |
US8632512B2 (en) | 2010-04-09 | 2014-01-21 | Kci Licensing, Inc. | Apparatuses, methods, and compositions for the treatment and prophylaxis of chronic wounds |
WO2011130246A2 (en) | 2010-04-13 | 2011-10-20 | Kci Licensing, Inc. | Compositions with reactive ingredients, and wound dressings, apparatuses, and methods |
US20190381222A9 (en) | 2010-04-16 | 2019-12-19 | Kci Licensing, Inc. | Reduced-Pressure Sources, Systems, And Methods Employing A Polymeric, Porous, Hydrophobic Material |
US8604265B2 (en) | 2010-04-16 | 2013-12-10 | Kci Licensing, Inc. | Dressings and methods for treating a tissue site on a patient |
GB201006986D0 (en) | 2010-04-27 | 2010-06-09 | Smith & Nephew | Wound dressing |
GB201008347D0 (en) | 2010-05-19 | 2010-07-07 | Smith & Nephew | Wound protection |
USRE48117E1 (en) | 2010-05-07 | 2020-07-28 | Smith & Nephew, Inc. | Apparatuses and methods for negative pressure wound therapy |
US10639404B2 (en) | 2010-06-03 | 2020-05-05 | Wound Healing Technologies, Llc | Wound dressing |
EP2582404B1 (en) | 2010-06-17 | 2020-08-19 | Covalon Technologies Inc. | Antimicrobial silicone-based wound dressings |
US8795257B2 (en) | 2010-07-19 | 2014-08-05 | Kci Licensing, Inc. | Systems and methods for electrically detecting the presence of exudate in dressings |
US8795246B2 (en) | 2010-08-10 | 2014-08-05 | Spiracur Inc. | Alarm system |
GB201015656D0 (en) | 2010-09-20 | 2010-10-27 | Smith & Nephew | Pressure control apparatus |
EP2635696B1 (en) | 2010-11-01 | 2017-11-29 | Becton Dickinson and Company | Gardnerella vaginalis assay |
WO2012069794A1 (en) | 2010-11-25 | 2012-05-31 | Smith & Nephew Plc | Composition i-ii and products and uses thereof |
GB201020236D0 (en) | 2010-11-30 | 2011-01-12 | Convatec Technologies Inc | A composition for detecting biofilms on viable tissues |
US8613733B2 (en) | 2010-12-15 | 2013-12-24 | Kci Licensing, Inc. | Foam dressing with integral porous film |
GB2488749A (en) | 2011-01-31 | 2012-09-12 | Systagenix Wound Man Ip Co Bv | Laminated silicone coated wound dressing |
EP3932327A1 (en) | 2011-02-04 | 2022-01-05 | University Of Massachusetts | Negative pressure wound closure device |
US9107990B2 (en) | 2011-02-14 | 2015-08-18 | Kci Licensing, Inc. | Reduced-pressure dressings, systems, and methods for use with linear wounds |
GB201105829D0 (en) | 2011-04-06 | 2011-05-18 | Convatec Technologies Inc | Antimicrobial compositions |
WO2012142002A1 (en) | 2011-04-12 | 2012-10-18 | Kci Licensing, Inc. | Evaporative fluid pouch and systems for use with body fluids |
GB201106491D0 (en) | 2011-04-15 | 2011-06-01 | Systagenix Wound Man Ip Co Bv | Patterened silicone coating |
US9382290B2 (en) | 2011-04-29 | 2016-07-05 | Kci Licensing, Inc. | Aptamer-modified polymeric materials for the binding of factors in a wound environment |
GB201108229D0 (en) | 2011-05-17 | 2011-06-29 | Smith & Nephew | Tissue healing |
US8708981B2 (en) | 2011-05-26 | 2014-04-29 | Kci Licensing, Inc. | Systems and methods of stimulation and activation of fluids for use with instillation therapy |
US9681993B2 (en) | 2011-06-07 | 2017-06-20 | Kci Licensing, Inc. | Solutions for bridging and pressure concentration reduction at wound sites |
EP3159020B1 (en) | 2011-06-07 | 2019-11-06 | Smith & Nephew plc | Wound contacting members |
WO2013016240A1 (en) | 2011-07-26 | 2013-01-31 | Kci Licensing, Inc. | Systems and methods for treating a tissue site with reduced pressure involving a reduced-pressure interface having a cutting element |
EP3622976B1 (en) | 2011-07-26 | 2023-11-01 | Smith & Nephew plc | Systems and methods for controlling operation of a reduced pressure therapy system |
GB201113515D0 (en) | 2011-08-04 | 2011-09-21 | Convatec Technologies Inc | A dressing |
EP2750723B1 (en) | 2011-08-30 | 2020-06-24 | Avery Dennison Corporation | Silicone absorbent adhesive layer |
DE102011081818A1 (en) | 2011-08-30 | 2013-02-28 | Beiersdorf Ag | Active skin coatings |
CA2844924C (en) | 2011-08-31 | 2019-12-03 | Kci Licensing, Inc. | Inline storage pouches for use with body fluids |
DE202011108806U1 (en) | 2011-09-02 | 2012-09-03 | BLüCHER GMBH | Wound dressing with permeable layer |
PL2567682T5 (en) | 2011-09-09 | 2018-06-29 | Paul Hartmann Ag | Abdominal wound dressing with application aid |
CN104023741B (en) | 2011-09-12 | 2017-11-17 | 普罗蒂吉生物医药有限责任公司 | Composition and dressing for treatment of wounds |
DK2572737T3 (en) | 2011-09-26 | 2016-06-27 | Bsn Medical Gmbh | improved wound dressing |
WO2013064831A1 (en) | 2011-11-01 | 2013-05-10 | Brightwake Limited | Wound dressings, and yarn useful therein |
US9393354B2 (en) | 2011-11-01 | 2016-07-19 | J&M Shuler Medical, Inc. | Mechanical wound therapy for sub-atmospheric wound care system |
CN103889378B (en) | 2011-11-15 | 2016-08-17 | 凯希特许有限公司 | There is Medical coating part, system and the method for the vapor transmission of heat enhancing |
US9132040B2 (en) | 2011-11-17 | 2015-09-15 | Ethicon, Inc. | Dressing device |
CA2855972C (en) | 2011-11-18 | 2019-08-27 | Kci Licensing, Inc. | Tissue treatment systems and methods having a porous substrate with a compressed region and an expanded region |
CN103917254B (en) | 2011-11-21 | 2016-08-24 | 凯希特许有限公司 | For identifying that wound filler is retained in the system of the part of tissue site, device and method |
US10940047B2 (en) | 2011-12-16 | 2021-03-09 | Kci Licensing, Inc. | Sealing systems and methods employing a hybrid switchable drape |
AU2013208271B2 (en) | 2012-01-10 | 2017-04-13 | Kci Licensing, Inc. | Systems and methods for delivering fluid to a wound therapy dressing |
EP2806908B1 (en) | 2012-01-25 | 2020-03-18 | The University of Akron | Fluorinated polymerizable hydrogels for wound dressings and methods of making same |
GB201201751D0 (en) | 2012-02-01 | 2012-03-14 | Haemostatix Ltd | Haemostatic wound dressing |
AU2013215067A1 (en) | 2012-02-02 | 2014-06-12 | Kci Licensing, Inc. | Foam structure wound inserts for directional granulation |
CA3019704C (en) | 2012-02-21 | 2020-06-16 | Kci Licensing, Inc. | A multi-orientation canister for use with a reduced pressure treatment system |
US10470936B2 (en) | 2012-02-29 | 2019-11-12 | Hollister Incorporated | Buffered adhesive compositions for skin-adhering medical products |
AU2013226154B2 (en) | 2012-02-29 | 2015-07-09 | Hollister Incorporated | Buffered adhesive compositions for skin-adhering products and methods of making same |
EP2636417B1 (en) | 2012-03-05 | 2017-04-26 | Lohmann & Rauscher GmbH | Wound treatment assembly and covering device for same |
AU2013234034B2 (en) | 2012-03-12 | 2017-03-30 | Smith & Nephew Plc | Reduced pressure apparatus and methods |
US10576037B2 (en) | 2012-03-14 | 2020-03-03 | MAM Holdings of West Florida, L.L.C. | Compositions comprising placental collagen for use in wound healing |
US9427505B2 (en) | 2012-05-15 | 2016-08-30 | Smith & Nephew Plc | Negative pressure wound therapy apparatus |
EP2852419B1 (en) | 2012-05-22 | 2019-11-20 | Smith & Nephew plc | Wound closure device |
HUE047600T2 (en) | 2012-05-23 | 2020-04-28 | Smith & Nephew | Apparatuses for negative pressure wound therapy |
GB201209745D0 (en) | 2012-05-31 | 2012-07-18 | Convatec Technologies Inc | Wound dressing |
JP6230166B2 (en) | 2012-06-03 | 2017-11-15 | ダニエル・エデュアード・クレイナー | Intraluminal negative pressure therapy device |
CA2868854A1 (en) | 2012-06-28 | 2014-01-03 | Kci Licensing, Inc. | Wound connection pad with rfid and integrated strain gauge pressure sensor |
MX357725B (en) | 2012-07-16 | 2018-07-19 | Univ Massachusetts | Negative pressure wound closure device. |
US10434210B2 (en) | 2012-07-19 | 2019-10-08 | Innovotech, Inc. | Antimicrobial silver iodate |
WO2014022400A1 (en) | 2012-07-30 | 2014-02-06 | Kci Licensing, Inc. | Reduced-pressure absorbent dressing, system for treating a tissue site, and method of manufacturing the dressing |
WO2014020440A1 (en) | 2012-08-01 | 2014-02-06 | Smith & Nephew Plc | Wound dressing |
WO2014028212A2 (en) | 2012-08-13 | 2014-02-20 | Kci Licensing, Inc. | Intelligent therapy system with evaporation management |
AU2013309002B2 (en) | 2012-08-28 | 2016-05-26 | 3M Innovative Properties Company | Chlorhexidine gluconate compositions, resin systems and articles |
EP2890412B1 (en) | 2012-08-31 | 2020-10-07 | Stryker European Holdings I, LLC | Hemostatic foam |
US20150202354A1 (en) | 2012-09-04 | 2015-07-23 | Integrated Healing Technolgies, LLC | Wound Dressing |
EP3260144B1 (en) | 2012-09-12 | 2020-01-29 | KCI Licensing, Inc. | Systems for collecting exudates in reduced-pressure therapy |
EP3693035B1 (en) | 2012-09-20 | 2024-02-07 | Lohmann & Rauscher GmbH | Vacuum treatment array |
GB201216928D0 (en) | 2012-09-21 | 2012-11-07 | I2R Medical Ltd | Portable medical device system |
US9877875B2 (en) | 2012-10-09 | 2018-01-30 | Parasol Medical LLC | Antimicrobial hydrogel formulation |
US9572968B2 (en) | 2012-10-11 | 2017-02-21 | Hanuman Pelican, Inc. | Compressive oxygen diffusive wound dressings |
JP6183831B2 (en) | 2012-10-23 | 2017-08-23 | 義之 小山 | Hydrogel forming material |
EP2912087A1 (en) | 2012-10-24 | 2015-09-02 | KCI Licensing, Inc. | Amine-functionalized polymeric compositions for medical devices |
EP3611205A1 (en) | 2012-10-24 | 2020-02-19 | KCI Licensing, Inc. | Sulfhydryl-functionalized polymeric compositions for medical devices |
CA2895896A1 (en) | 2012-12-20 | 2014-06-26 | Convatec Technologies Inc. | Processing of chemically modified cellulosic fibres |
CN104884009A (en) | 2012-12-21 | 2015-09-02 | 3M创新有限公司 | Medical dressing comprising a flap |
JP6474731B2 (en) | 2013-01-03 | 2019-02-27 | ケーシーアイ ライセンシング インコーポレイテッド | Hygroscopic seal |
GB201309369D0 (en) | 2013-05-24 | 2013-07-10 | Smith & Nephew | Moisture indicating system |
EP3607977A1 (en) | 2013-01-16 | 2020-02-12 | KCI Licensing, Inc. | Ion exchange enhanced absorbent systems |
US20140256925A1 (en) | 2013-03-05 | 2014-09-11 | The Penn State Research Foundation | Composite materials |
WO2014163733A2 (en) | 2013-03-13 | 2014-10-09 | Kci Licensing, Inc. | Expandable fluid collection canister |
WO2014158529A1 (en) | 2013-03-14 | 2014-10-02 | Kci Licensing, Inc. | A fluid collection canister with integrated moisture trap |
EP2968704B1 (en) | 2013-03-14 | 2020-07-15 | KCI Licensing, Inc. | Negative pressure therapy with dynamic profile capability |
EP2968703B1 (en) | 2013-03-14 | 2019-04-10 | KCI Licensing, Inc. | Micro-porous conduit |
EP3804742A1 (en) | 2013-03-15 | 2021-04-14 | 3M Innovative Properties Company | Wound healing compositions |
US11452698B2 (en) | 2013-03-15 | 2022-09-27 | Smith & Nephew, Inc. | Dissolvable gel-forming film for delivery of active agents |
US10492956B2 (en) | 2013-03-15 | 2019-12-03 | Kci Licensing, Inc. | Topical vacuum-press surgical incisional dressings, surgical adjuncts, hybrids and composites |
CA2907220A1 (en) | 2013-03-15 | 2014-10-16 | Euromed Inc. | Adhesive composition comprising silica |
CA2902392C (en) | 2013-03-15 | 2023-08-01 | Smith & Nephew Plc | Wound dressing for negative pressure wound therapy |
US20160038347A1 (en) | 2013-03-15 | 2016-02-11 | Stb, Ltd. | Compositions having cylindrical volume, methods, and applicators for sealing injuries |
US20160120706A1 (en) | 2013-03-15 | 2016-05-05 | Smith & Nephew Plc | Wound dressing sealant and use thereof |
GB2512841B (en) | 2013-04-08 | 2020-07-15 | Brightwake Ltd | Absorbent wound dressings |
HUE048583T2 (en) | 2013-04-08 | 2020-09-28 | Univ Yeditepe | Polymer based hydrogel |
WO2014169250A1 (en) | 2013-04-11 | 2014-10-16 | President And Fellows Of Harvard College | Prefabricated alginate-drug bandages |
US10687983B2 (en) | 2013-04-17 | 2020-06-23 | Mölnlycke Health Care Ab | Wound pad |
US10016380B2 (en) | 2013-05-01 | 2018-07-10 | Lanny Leo Johnson | Antimicrobials and methods of use thereof |
US9884087B1 (en) | 2013-05-03 | 2018-02-06 | Chan Soon-Shiong Nanthealth Foundation | Compositions and methods of improved wound healing |
AU2014266943B2 (en) | 2013-05-10 | 2018-03-01 | Smith & Nephew Plc | Fluidic connector for irrigation and aspiration of wounds |
GB201308770D0 (en) | 2013-05-15 | 2013-06-26 | Convatec Technologies Inc | Wound Dressing Comprising an Antimicrobial Composition |
WO2014190038A2 (en) | 2013-05-22 | 2014-11-27 | The Penn State Research Foundation | Wound dressings and applications thereof |
US10772767B2 (en) | 2013-06-28 | 2020-09-15 | 3M Innovative Properties Company | Fibrin-coated wound dressing |
US9993577B2 (en) | 2013-07-01 | 2018-06-12 | Trustees Of Boston University | Dissolvable hydrogel compositions for wound management and methods of use |
US10765774B2 (en) | 2013-07-09 | 2020-09-08 | Ethicon, Inc. | Hemostatic pad assembly kit and method |
KR20160040242A (en) | 2013-08-05 | 2016-04-12 | 쓰리엠 이노베이티브 프로퍼티즈 컴파니 | A support device with a contained cushioning element |
BR112016002822B1 (en) | 2013-08-12 | 2022-07-12 | Bsn Medical Gmbh | ARTICLE FOR PLAN WOUND TREATMENT AND ITS USE |
US20160193244A1 (en) | 2013-08-13 | 2016-07-07 | Seikagaku Corporation | Drug containing cationized chitosan |
EP3578209B1 (en) | 2013-08-26 | 2023-12-20 | 3M Innovative Properties Company | Dressing interface with moisture controlling feature and sealing function |
GB2518199A (en) | 2013-09-13 | 2015-03-18 | Xiros Ltd | Method of producing a swellable polymer fibre |
US10342891B2 (en) | 2013-09-19 | 2019-07-09 | Medline Industries, Inc. | Wound dressing containing saccharide and collagen |
WO2015051018A2 (en) | 2013-10-02 | 2015-04-09 | Kci Licensing, Inc. | Disposable reduced-pressure therapy system with mechanical feedback |
CA2926370C (en) | 2013-10-10 | 2018-06-26 | F. Hoffmann-La Roche Ag | Carrier system for an object worn on the body and method of production |
SG11201603058UA (en) | 2013-10-18 | 2016-05-30 | Agency Science Tech & Res | Nanoparticle-containing hydrogels |
CN106170275B (en) | 2013-10-21 | 2021-05-07 | 史密夫和内修有限公司 | Negative pressure wound closure device |
GB201318842D0 (en) | 2013-10-24 | 2013-12-11 | First Water Ltd | Flexible hydrogel wound dressings |
WO2015065742A1 (en) | 2013-10-28 | 2015-05-07 | Kci Licensing, Inc. | Hybrid sealing tape |
EP3656362A1 (en) | 2013-10-30 | 2020-05-27 | KCI Licensing, Inc. | Condensate absorbing and dissipating system related application |
MX351120B (en) | 2013-11-07 | 2017-10-03 | Bsn Medical Gmbh | Medical dressing. |
GB2522178B (en) | 2013-11-12 | 2018-07-18 | First Water Ltd | Multilayer composition |
WO2015075406A1 (en) | 2013-11-19 | 2015-05-28 | Lipopeptide Ab | New treatment of chronic ulcers |
SG10201811454WA (en) | 2013-12-12 | 2019-01-30 | Innovation Technologies Inc | Materials and methods for controlling infections |
CN105873549B (en) | 2013-12-31 | 2017-10-27 | 3M创新有限公司 | Conformal drape covering dressing |
WO2015112810A1 (en) | 2014-01-24 | 2015-07-30 | Avent, Inc. | Traumatic wound dressing system with conformal cover |
EP3939554A1 (en) | 2014-02-11 | 2022-01-19 | 3M Innovative Properties Company | Devices for applying closed incision negative pressure wound therapy |
DE102014202578A1 (en) | 2014-02-12 | 2015-08-13 | Aesculap Ag | Medical product and process for its preparation |
AU2015218302B2 (en) | 2014-02-14 | 2019-11-07 | Atomic Medical Innovations, Inc. | Systems and methods for tissue healing |
US10610623B2 (en) | 2014-02-14 | 2020-04-07 | Kci Licensing, Inc. | Systems and methods for increasing absorbent capacity of a dressing |
WO2015130471A1 (en) | 2014-02-28 | 2015-09-03 | Kci Licensing, Inc. | Hybrid drape having a gel-coated perforated mesh |
GB201404021D0 (en) | 2014-03-05 | 2014-04-23 | Lumina Adhesives Ab | Low cytotoxity switchable adhesive compositions, medical dressings and skin coverings, and methods of treatment using same |
US20150367019A1 (en) | 2014-03-12 | 2015-12-24 | Stb, Ltd. | Hemostatic compositions and methods |
CA2939277C (en) | 2014-03-21 | 2022-07-26 | Bridget DONAVAN | Wound management system and methods of using |
IN2014DE00838A (en) | 2014-03-24 | 2015-10-02 | Datt Mediproducts Ltd | |
GB2524510B (en) | 2014-03-25 | 2020-02-19 | Brightwake Ltd | Wound dressing impregnated with honey |
AU2014393037B2 (en) | 2014-04-30 | 2019-02-28 | Matoke Holdings Limited | Antimicrobial compositions |
US10406266B2 (en) | 2014-05-02 | 2019-09-10 | Kci Licensing, Inc. | Fluid storage devices, systems, and methods |
WO2015172111A1 (en) | 2014-05-09 | 2015-11-12 | Kci Licensing, Inc. | Disruptive dressing for use with negative pressure and fluid instillation |
CN111134959B (en) | 2014-05-09 | 2022-08-16 | 3M创新知识产权公司 | Dressing with a shrink layer for a linear tissue site |
US10398610B2 (en) | 2014-05-13 | 2019-09-03 | The Procter & Gamble Company | Absorbent article with dual core |
GB2526267B (en) | 2014-05-14 | 2020-10-28 | Brightwake Ltd | Dressing for surgical drain |
CN106535842B (en) | 2014-06-05 | 2020-07-17 | 凯希特许有限公司 | Dressing with fluid acquisition and distribution features |
KR101743274B1 (en) | 2014-06-12 | 2017-06-02 | 주식회사 엘지화학 | Super absorbent polymer |
JP6586431B2 (en) | 2014-06-18 | 2019-10-02 | スミス アンド ネフュー ピーエルシーSmith & Nephew Public Limited Company | Wound dressing and method of treatment |
ES2790800T3 (en) | 2014-06-18 | 2020-10-29 | Toray Industries | Laminate and manufacturing procedure |
WO2016006457A1 (en) | 2014-07-07 | 2016-01-14 | 株式会社村田製作所 | Negative-pressure closure therapy device |
WO2016007692A1 (en) | 2014-07-09 | 2016-01-14 | Lubrizol Advanced Materials, Inc. | Hydrogel compositions |
CA2954467A1 (en) | 2014-07-10 | 2016-01-28 | Smith & Nephew Plc | Improvements in and relating to devices |
CN107075165A (en) | 2014-07-24 | 2017-08-18 | 亚瑟罗凯尔公司 | Many sugarwhips of elasticity and application thereof |
US20200289723A1 (en) | 2014-07-31 | 2020-09-17 | Smith & Nephew, Inc. | Reduced pressure therapy apparatus construction and control |
EP3659638A1 (en) | 2014-07-31 | 2020-06-03 | Smith & Nephew, Inc. | Systems and methods for applying reduced pressure therapy |
US20170209615A1 (en) | 2014-08-04 | 2017-07-27 | Hospital Sant Joan De Deu | System for the immediate release of active agents |
US9770369B2 (en) | 2014-08-08 | 2017-09-26 | Neogenix, Llc | Wound care devices, apparatus, and treatment methods |
US10583042B2 (en) | 2014-08-08 | 2020-03-10 | Neogenix, Llc | Wound care devices, apparatus, and treatment methods |
CA2955060A1 (en) | 2014-08-11 | 2016-02-18 | Kci Licensing, Inc. | Protease modulating wound interface layer for use with negative pressure wound therapy |
WO2016040489A1 (en) | 2014-09-09 | 2016-03-17 | Shaoyi Jiang | Functionalized zwitterionic and mixed charge polymers, related hydrogels, and methds for their use |
EP2995287A1 (en) | 2014-09-11 | 2016-03-16 | Mölnlycke Health Care AB | Medical dressing |
EP2995324A1 (en) | 2014-09-11 | 2016-03-16 | Mölnlycke Health Care AB | Medical dressing |
JP6793117B2 (en) | 2014-10-01 | 2020-12-02 | スリーエム イノベイティブ プロパティズ カンパニー | Porous devices, kits, and methods for debridement |
EP3446665B1 (en) | 2014-10-06 | 2021-12-15 | 3M Innovative Properties Company | Ion exchange absorbent systems, apparatuses |
EP3204057B1 (en) | 2014-10-06 | 2019-12-04 | GATT Technologies B.V. | Tissue-adhesive porous haemostatic product |
US9855364B2 (en) | 2014-10-15 | 2018-01-02 | Allison Coomber | Wound dressing materials incorporating anthocyanins derived from fruit or vegetable sources |
EP3011978B1 (en) | 2014-10-24 | 2017-05-10 | Sefar AG | Wound dressing material and method for producing the same |
US10485893B2 (en) | 2014-11-13 | 2019-11-26 | Sarasota Medical Products, Inc. | Antimicrobial hydrocolloid dressing containing sequestered peroxide and preparation thereof |
EP3023083A1 (en) | 2014-11-20 | 2016-05-25 | Mölnlycke Health Care AB | Wound dressings |
WO2016086088A2 (en) | 2014-11-25 | 2016-06-02 | Northwestern University | Wound healing through sirt1 overexpression |
JP2017536402A (en) | 2014-12-04 | 2017-12-07 | スリーエム イノベイティブ プロパティズ カンパニー | Antibacterial composition comprising bioglass |
CN107206119B (en) | 2014-12-09 | 2021-01-29 | 实体科学公司 | Medical device coating with biocompatible layer |
TW201622668A (en) | 2014-12-16 | 2016-07-01 | 準訊生醫股份有限公司 | Long-term effective patch structure |
WO2016100098A1 (en) | 2014-12-17 | 2016-06-23 | Kci Licensing, Inc. | Dressing with offloading capability |
CN107107598B (en) | 2014-12-18 | 2019-11-22 | 3M创新有限公司 | The method for handling bonded laminate patch |
EP3234052B1 (en) | 2014-12-19 | 2020-09-16 | 3M Innovative Properties Company | Adhesive article comprising a poly(meth)acrylate-based primer layer and methods of making same |
AU2015370586B2 (en) | 2014-12-22 | 2020-07-16 | Smith & Nephew Plc | Negative pressure wound therapy apparatus and methods |
US10828403B2 (en) | 2014-12-29 | 2020-11-10 | Smith & Nephew Plc | Negative pressure wound therapy apparatus and methods for operating the apparatus |
US10549016B2 (en) | 2014-12-30 | 2020-02-04 | Smith & Nephew, Inc. | Blockage detection in reduced pressure therapy |
WO2016109418A1 (en) | 2014-12-30 | 2016-07-07 | 3M Innovative Properties Company | Wound dressing with multiple adhesive layers |
US10660851B2 (en) | 2015-01-02 | 2020-05-26 | Rxos Medical | Polyfunctional radical scavenger hydrogel formulation |
KR101949455B1 (en) | 2015-01-07 | 2019-02-18 | 주식회사 엘지화학 | Superabsorbent Polymers with Improved Anticaking Property And Method Of Preparing The Same |
GB201500430D0 (en) | 2015-01-12 | 2015-02-25 | Univ Birmingham | Dressing |
US10806819B2 (en) | 2015-01-15 | 2020-10-20 | Marshall University Research Corporation | Wound coverings comprising vitamin d and related methods |
GB201501334D0 (en) | 2015-01-27 | 2015-03-11 | Medtrade Products Ltd | Composition for a wound dressing |
GB201501330D0 (en) | 2015-01-27 | 2015-03-11 | Medtrade Products Ltd | Composition for a wound dressing |
GB201501333D0 (en) | 2015-01-27 | 2015-03-11 | Medtrade Products Ltd | Composition for a wound dressing |
US10500235B2 (en) | 2015-01-29 | 2019-12-10 | San Melix Laboratories, Inc. | Wound healing compositions comprising buckwheat honey and methylglyoxal and methods of use |
US20200093756A1 (en) | 2015-01-29 | 2020-03-26 | Sanmelix Laboratories, Inc. | Buckwheat honey and povidone-iodine wound-healing dressing |
KR101841469B1 (en) | 2015-01-30 | 2018-03-23 | (주)메디팁 | Method for manufacturing wound covering material using biopolymer and wound covering material using biopolymer manufactured by the same |
WO2016126444A1 (en) | 2015-02-02 | 2016-08-11 | Kci Licensing, Inc. | Customizable closed tissue site dressing for improved postoperative removal |
US11207458B2 (en) | 2015-02-02 | 2021-12-28 | Kci Licensing, Inc. | Pressure-operated switch |
US10512707B2 (en) | 2015-02-02 | 2019-12-24 | University Of Southern California | System for sutureless closure of scleral perforations and other ocular tissue discontinuities |
CA2975197A1 (en) | 2015-02-03 | 2016-08-11 | Matoke Holdings Limited | Antimicrobial fibers and compositions |
GB201501965D0 (en) | 2015-02-05 | 2015-03-25 | Lumina Adhesives Ab | Polyurethane based switchable adhesives |
US10485892B2 (en) | 2015-03-10 | 2019-11-26 | Covalon Technologies Inc. | Method for local reduction of microbial skin flora |
EP3265137B1 (en) | 2015-03-10 | 2020-06-17 | Covalon Technologies Inc. | Method for local reduction of microbial skin flora |
WO2016145237A1 (en) | 2015-03-11 | 2016-09-15 | Yu Fu-Shin X | Composition and methods to promote wound healing |
GB201506236D0 (en) | 2015-04-13 | 2015-05-27 | Jellagen Pty Ltd | Modified collagen, methods of manufacture thereof |
EP3085344B1 (en) | 2015-04-21 | 2019-12-04 | Mölnlycke Health Care AB | A wound pad and a self-adhesive member comprising a wound pad |
CN107847633B (en) | 2015-04-23 | 2021-06-29 | 佛罗里达大学研究基金会公司 | Bilayer device for enhanced healing |
ES2769811T3 (en) | 2015-04-27 | 2020-06-29 | Smith & Nephew | Reduced pressure devices |
WO2016179575A1 (en) | 2015-05-07 | 2016-11-10 | Kci Liensing, Inc. | A controlled release iodine structure for use with wound care |
US10507259B2 (en) | 2015-05-08 | 2019-12-17 | First Quality Retail Services, Llc | Flexible absorbent pad |
US20190298580A1 (en) | 2015-05-08 | 2019-10-03 | Kci Licensing, Inc. | Low-acuity dressing with integral pump |
US11246975B2 (en) | 2015-05-08 | 2022-02-15 | Kci Licensing, Inc. | Low acuity dressing with integral pump |
EP3294158B1 (en) | 2015-05-08 | 2020-05-06 | KCI Licensing, Inc. | Wound debridement by irrigation with ultrasonically activated microbubbles |
EP3093031A1 (en) | 2015-05-11 | 2016-11-16 | 3M Innovative Properties Company | Wound care system |
EP3092987A1 (en) | 2015-05-11 | 2016-11-16 | 3M Innovative Properties Company | System for treatment of wounds using serum |
WO2016184918A1 (en) | 2015-05-18 | 2016-11-24 | Smith & Nephew Plc | Heat-assisted pumping systems for use in negative pressure wound therapy |
US10076594B2 (en) | 2015-05-18 | 2018-09-18 | Smith & Nephew Plc | Fluidic connector for negative pressure wound therapy |
WO2016187666A1 (en) | 2015-05-26 | 2016-12-01 | Monash University | Antibacterial bismuth complexes |
KR101889677B1 (en) | 2015-06-12 | 2018-08-17 | 제이에프이미네라르 가부시키가이샤 | Therapeutic agent for skin wound or rough skin |
US11559421B2 (en) | 2015-06-25 | 2023-01-24 | Hill-Rom Services, Inc. | Protective dressing with reusable phase-change material cooling insert |
EP3313469B1 (en) | 2015-06-29 | 2019-01-02 | KCI Licensing, Inc. | Apparatus for negative-pressure therapy and irrigation |
WO2017011204A1 (en) | 2015-07-14 | 2017-01-19 | Kci Licensing, Inc. | Medical dressing interface devices, systems, and methods |
EP3117806B1 (en) | 2015-07-16 | 2020-06-10 | Lohmann & Rauscher GmbH | Wound treatment assembly |
EP3708193B1 (en) | 2015-07-24 | 2023-02-22 | Mölnlycke Health Care AB | Absorbent antimicrobial wound dressings |
US10583228B2 (en) | 2015-07-28 | 2020-03-10 | J&M Shuler Medical, Inc. | Sub-atmospheric wound therapy systems and methods |
US10682257B2 (en) | 2015-07-29 | 2020-06-16 | Evophancie Biotech Ltd | Biological fiber composite dressing |
WO2017023623A1 (en) | 2015-07-31 | 2017-02-09 | Wu, Karl | Composition for use in promoting wound healing |
EP3334449B1 (en) | 2015-08-10 | 2021-09-29 | The Medical Research, Infrastructure, And Health Services Fund Of The Tel Aviv Medical Center | Methods and pharmaceutical compositions for improving wound healing using cd24 |
KR101787192B1 (en) | 2015-08-12 | 2017-10-18 | 주식회사 제네웰 | Antimicrbacterial dressing material and method for preparing thereof |
EP3135304A1 (en) | 2015-08-26 | 2017-03-01 | Mölnlycke Health Care AB | Foamed silicone in wound care |
JP6875378B2 (en) | 2015-08-31 | 2021-05-26 | スリーエム イノベイティブ プロパティズ カンパニー | Negative pressure wound therapy dressing containing (meth) acrylate pressure-sensitive adhesive with enhanced adhesion to wet surfaces |
US10617608B2 (en) | 2015-09-25 | 2020-04-14 | Lotte Fine Chemical Co., Ltd. | Composition for hydrogel sheet, hydrogel sheet manufactured therefrom, and method for manufacturing same |
CN108137841B (en) | 2015-09-30 | 2021-07-06 | 3M创新有限公司 | Hydrogel compositions bonded to polymeric substrates |
GB2543307B (en) | 2015-10-14 | 2020-12-09 | Selentus Science Ltd | Haemostatic device |
AU2016344202B2 (en) | 2015-10-30 | 2020-10-29 | Advanced Dressings, LLC | Tissue treatment device and method |
EP3373984B1 (en) | 2015-11-13 | 2020-12-23 | 3M Innovative Properties Company | Anti-microbial articles and methods of using same |
GB2544342B (en) | 2015-11-13 | 2020-06-03 | First Water Ltd | Compositions for application to wounds |
WO2017087173A1 (en) | 2015-11-18 | 2017-05-26 | Kci Licensing, Inc. | Medical drapes and methods for reducing trauma on removal |
WO2017087163A1 (en) | 2015-11-20 | 2017-05-26 | Kci Licensing, Inc. | Medical system with flexible fluid storage bridge |
GB201520990D0 (en) | 2015-11-27 | 2016-01-13 | Edixomed Ltd | Dressing system |
FR3044893B1 (en) | 2015-12-09 | 2018-05-18 | Emile Droche | DRESSING FOR SKIN CARE IN A WET MEDIUM |
US10575991B2 (en) | 2015-12-15 | 2020-03-03 | University Of Massachusetts | Negative pressure wound closure devices and methods |
EP3397219B1 (en) | 2015-12-30 | 2020-10-21 | Smith & Nephew plc | Absorbent negative pressure wound therapy dressing |
EP3187204B1 (en) | 2015-12-30 | 2020-07-15 | Paul Hartmann AG | Methods and devices for controlling negative pressure wound therapy |
CA3009878A1 (en) | 2015-12-30 | 2017-07-06 | Smith & Nephew Plc | Negative pressure wound therapy apparatus |
WO2017119996A1 (en) | 2016-01-06 | 2017-07-13 | Kci Liecensing, Inc. | System and methods for the treatment of wounds with dressing having closed cells |
US10426809B2 (en) | 2016-01-12 | 2019-10-01 | Council Of Scientific & Industrial Research | Nanobiocomposite formulation for wound healing and a process for the preparation thereof |
DE102016000569B3 (en) | 2016-01-20 | 2017-06-22 | Lohmann & Rauscher Gmbh | Method for producing a film tube |
US10918770B2 (en) | 2016-02-12 | 2021-02-16 | Corning Incorporated | Vacuum assisted wound closure assembly and methods of irradiating a wound using the same |
FR3047901B1 (en) | 2016-02-22 | 2018-02-23 | Universite Pierre Et Marie Curie (Paris 6) | BIOMATERIAL COMPOSITIONS WITH CONTROLLED RELEASE OF ACTIVE INGREDIENTS |
US20190060127A1 (en) | 2016-03-01 | 2019-02-28 | Kci Licensing, Inc. | Drape For Use With Medical Therapy Systems |
KR101958014B1 (en) | 2016-03-14 | 2019-03-13 | 주식회사 엘지화학 | Preparation method of super absorbent polymer |
FR3048885A1 (en) | 2016-03-17 | 2017-09-22 | Bluestar Silicones France | SILICONE ADHESIVE GEL WITH SKIN |
EP3429643A1 (en) | 2016-03-18 | 2019-01-23 | KCI USA, Inc. | Antimicrobial wound dressing |
KR101959547B1 (en) | 2016-03-25 | 2019-03-18 | 주식회사 엘지화학 | Preparation method for super absorbent polymer |
US20200023102A1 (en) | 2016-04-05 | 2020-01-23 | Patrick Kenneth Powell | Wound therapy system |
GB201608099D0 (en) | 2016-05-09 | 2016-06-22 | Convatec Technologies Inc | Negative pressure wound dressing |
EP3246050A1 (en) | 2016-05-17 | 2017-11-22 | BSN medical GmbH | Wound or skin patch |
EP4194017A1 (en) | 2016-05-31 | 2023-06-14 | Octapharma AG | Plasma-based films and methods for making and using the same |
US20200282100A1 (en) | 2016-06-01 | 2020-09-10 | 3-D Matrix, Ltd. | Hemostatic Dressings with Self-Assembling Peptide Hydrogels |
KR20190028467A (en) | 2016-07-08 | 2019-03-18 | 컨바텍 테크놀러지스 인크 | Body fluid collecting device |
TWI673056B (en) | 2016-07-22 | 2019-10-01 | 大江生醫股份有限公司 | Bacterium-containing hydrogel and method of making the same |
US10076552B2 (en) | 2016-08-09 | 2018-09-18 | DATT MEDIPRODUCTS LIMITED and DATT LIFE SCIENCE PVT. LTD. | Multifunctional formulation comprised of natural ingredients and method of preparation/manufacturing thereof |
WO2018031761A1 (en) | 2016-08-10 | 2018-02-15 | Argentum Medical, Llc | Antimicrobial hydrogel dressings |
DE102016114819A1 (en) | 2016-08-10 | 2018-02-15 | Paul Hartmann Ag | Absorbent body for endoluminal negative pressure therapy |
ES2833899T3 (en) | 2016-08-10 | 2021-06-16 | Adv Med Solutions Ltd | Wound dressing |
US20180056087A1 (en) | 2016-08-26 | 2018-03-01 | Adolfo Ribeiro | Wearable Micro-LED Healing Bandage |
US11111362B2 (en) | 2016-09-26 | 2021-09-07 | Becton, Dickinson And Company | Breathable films with microbial barrier properties |
US10590184B2 (en) | 2016-09-26 | 2020-03-17 | National Yang-Ming University | Process for a preparation of the modified porcine plasma fibronectin for enhance wound healing |
IT201600096247A1 (en) | 2016-09-26 | 2018-03-26 | Emodial S R L | Polyurethane-based bandage and hydrogel comprising chlorhexidine |
US10293080B2 (en) | 2016-10-05 | 2019-05-21 | The Arizona Board Of Regents On Behalf Of Northern Arizona University | Ionic liquids that sterilize and prevent biofilm formation in skin wound healing devices |
EP3522941A4 (en) | 2016-10-05 | 2020-06-17 | 3M Innovative Properties Company | Fibrinogen composition, method and wound articles |
GB2555584B (en) | 2016-10-28 | 2020-05-27 | Smith & Nephew | Multi-layered wound dressing and method of manufacture |
US20190298249A1 (en) | 2016-11-02 | 2019-10-03 | Conopco, Inc., D/B/A Unilever | Malodour sampling method |
CA3042673A1 (en) | 2016-11-02 | 2018-05-11 | Smith & Nephew Inc. | Wound closure devices |
WO2018089563A1 (en) | 2016-11-11 | 2018-05-17 | 3M Innovative Properties Company | Trimmable conformable wound dressing |
US11590255B2 (en) | 2016-11-11 | 2023-02-28 | Avery Dennison Corporation | Rubber-based soft gel skin adhesives |
WO2018094061A1 (en) | 2016-11-18 | 2018-05-24 | Kci Licensing, Inc. | Medical system and dressing for use under compression |
US10426874B2 (en) | 2016-12-02 | 2019-10-01 | Apex Medical Corp. | Wound management assembly and negative pressure wound therapy system |
WO2018102322A1 (en) | 2016-12-02 | 2018-06-07 | 3M Innovative Properties Company | Muscle or joint support article with bump |
US11446185B2 (en) | 2016-12-02 | 2022-09-20 | 3M Innovative Properties Company | Muscle or joint support article |
US10500104B2 (en) | 2016-12-06 | 2019-12-10 | Novomer, Inc. | Biodegradable sanitary articles with higher biobased content |
EP3551195A4 (en) | 2016-12-06 | 2020-07-15 | Sami Shamoon College Of Engineering (R.A) | Topical antimicrobial formulations containing monovalent copper ions and systems for generating monovalent copper ions |
WO2018107130A1 (en) | 2016-12-09 | 2018-06-14 | Sanvio,Inc. | Composition for treating wounds and other dermatological conditions |
US11806217B2 (en) | 2016-12-12 | 2023-11-07 | Smith & Nephew Plc | Wound dressing |
DK3335696T3 (en) | 2016-12-15 | 2020-03-16 | Upm Kymmene Corp | Process for drying cell-free tissue extract in a hydrogel comprising nanofibrillar cellulose and a dried hydrogel comprising nanofibrillar cellulose and cell-free tissue extract |
EP3335740B1 (en) | 2016-12-15 | 2024-07-24 | UPM-Kymmene Corporation | Medical hydrogel |
DK3335695T3 (en) | 2016-12-15 | 2020-04-20 | Upm Kymmene Corp | Process for freeze-drying hydrogel comprising nanofibrillar cellulose, freeze-dried medical hydrogel comprising nanofibrillar cellulose and hydrogel comprising nanofibrillar cellulose |
EP3338813B1 (en) | 2016-12-20 | 2020-01-29 | BSN Medical GmbH | Multi-layer wound care product with perforated release layer |
CA3048014A1 (en) | 2016-12-22 | 2018-06-28 | Applied Tissue Technologies Llc | Devices and methods for wound treatment |
EP3401354B1 (en) | 2016-12-22 | 2020-04-08 | LG Chem, Ltd. | Super absorbent polymer manufacturing method and super absorbent polymer |
CN110099653A (en) | 2016-12-23 | 2019-08-06 | 卡尔冈碳素公司 | Active carbon composite wound dressing |
DE102016125579A1 (en) | 2016-12-23 | 2018-06-28 | Paul Hartmann Ag | Hydrous hydrogel composition comprising elemental silver particles |
US20200114040A1 (en) | 2016-12-28 | 2020-04-16 | Kci Usa, Inc. | Antimicrobial wound dressings |
WO2018124303A1 (en) | 2016-12-29 | 2018-07-05 | アルケア株式会社 | Foam body and foam body composition |
CN110267630B (en) | 2017-01-09 | 2021-12-28 | 3M创新知识产权公司 | Wound dressing layer for improved fluid removal |
TWI621453B (en) | 2017-01-13 | 2018-04-21 | 廈門聖慈醫療器材有限公司 | Suction disc |
KR102039701B1 (en) | 2017-01-19 | 2019-11-26 | 주식회사 리포바이오메드 | A pad for treating and alleviating the skin diseases associated with the exudation of plasma protein comprising atopic disease |
EP3570798A1 (en) | 2017-01-23 | 2019-11-27 | Medela Holding AG | Porous wound insert for use in negative pressure therapy |
US20190365948A1 (en) | 2017-01-27 | 2019-12-05 | Aziyo Biologics, Inc. | Lyophilized placental composite sheet and uses thereof |
CN110248631B (en) | 2017-02-06 | 2021-12-10 | 巴斯夫欧洲公司 | Fluid-absorbent article |
WO2018150267A2 (en) | 2017-02-15 | 2018-08-23 | Smith & Nephew Pte. Limited | Negative pressure wound therapy apparatuses and methods for using the same |
WO2018149835A1 (en) | 2017-02-16 | 2018-08-23 | Covestro Deutschland Ag | Method for producing an adhesive-free wound contact composite material |
WO2018156730A1 (en) | 2017-02-22 | 2018-08-30 | Cornell University | Mechanical vacuum dressing for mechanically managing, protecting and suctioning small incisional wounds |
WO2018158250A1 (en) | 2017-02-28 | 2018-09-07 | T.J.Smith And Nephew,Limited | Multiple dressing negative pressure wound therapy system |
WO2018161036A1 (en) | 2017-03-03 | 2018-09-07 | Loma Linda University Health | Compositions and methods for promoting hemostasis |
CA3055664A1 (en) | 2017-03-08 | 2018-09-13 | Smith & Nephew Plc | Negative pressure wound therapy device control in presence of fault condition |
EP3592212B1 (en) | 2017-03-09 | 2024-08-07 | Smith & Nephew plc | Wound dressing |
WO2018163093A1 (en) | 2017-03-09 | 2018-09-13 | Secretary, Department Of Biotechnology | A wound dressing for combined negative pressure and fluid delivery system |
EP3378450A1 (en) | 2017-03-22 | 2018-09-26 | Mölnlycke Health Care AB | Method for manufacturing a wound dressing and a wound dressing |
JP7150744B2 (en) | 2017-03-29 | 2022-10-11 | スリーエム イノベイティブ プロパティズ カンパニー | Hydrogel composition bound to a polymer matrix |
US11452809B2 (en) | 2017-04-04 | 2022-09-27 | Kcl Licensing, Inc. | Apparatuses, systems, and methods for the treatment of a tissue site with negative pressure and oxygen |
BR112019020766B1 (en) | 2017-04-04 | 2022-11-29 | Anti-Plasmin Technologies, Llc | COMPOSITION TO IMPROVE A NON-SURGICAL MEDICAL TREATMENT |
KR101852718B1 (en) | 2017-04-04 | 2018-05-18 | 주식회사 제네웰 | Kit for pain reduction of incision site after surgical operation |
GB201800057D0 (en) | 2018-01-03 | 2018-02-14 | Smith & Nephew Inc | Component Positioning And stress Relief For Sensor Enabled Wound Dressings |
CN108721677B (en) | 2017-04-17 | 2021-11-19 | 广西美丽肤医疗器械有限公司 | Composite material |
WO2018195101A1 (en) | 2017-04-19 | 2018-10-25 | Smith & Nephew, Inc. | Negative pressure wound therapy canisters |
DE102017003826A1 (en) | 2017-04-20 | 2018-10-25 | Lohmann & Rauscher Gmbh | Wound treatment arrangement for the negative pressure therapy |
US20200085625A1 (en) | 2017-05-04 | 2020-03-19 | Klox Technologies Inc. | Absorbent biophotonic devices and systems for wound healing |
MY198078A (en) | 2017-05-10 | 2023-07-31 | Toray Industries | Medical device |
JP7069219B2 (en) | 2017-05-10 | 2022-05-17 | メンリッケ・ヘルス・ケア・アーベー | Composite form in wound healing |
CN110870021A (en) | 2017-05-15 | 2020-03-06 | 史密夫及内修公开有限公司 | Negative pressure wound therapy system using Euler video amplification |
US11850126B2 (en) | 2017-05-16 | 2023-12-26 | 3M Innovative Properties Company | Absorbent negative-pressure dressing system for use with post-surgical breast wounds |
EP3634506A4 (en) | 2017-05-17 | 2021-03-10 | UVIC Industry Partnerships Inc. | Wound covering for wound monitoring and therapeutic agent delivery |
WO2018213534A1 (en) | 2017-05-19 | 2018-11-22 | Kci Usa, Inc. | Dressings for filtering wound fluids |
EP3630029A1 (en) | 2017-05-22 | 2020-04-08 | KCI USA, Inc. | Extensible dressings |
WO2018217621A1 (en) | 2017-05-22 | 2018-11-29 | Kci Usa, Inc. | Elastically deformable wound dressings |
US20200197227A1 (en) | 2017-05-22 | 2020-06-25 | Kci Usa, Inc. | Post-operative surgical wound dressing |
EP3409248B1 (en) | 2017-06-01 | 2019-11-06 | Absorbest AB | Wound dressing |
CA3065521A1 (en) | 2017-06-07 | 2018-12-13 | Kci Licensing, Inc. | Multi-layer wound filler for extended wear time |
BR112019025041A2 (en) | 2017-06-07 | 2020-06-16 | Kci Licensing, Inc | DRESSING FOR USE WITH NEGATIVE PRESSURE TREATMENT, DRESSING KIT FOR USE WITH NEGATIVE PRESSURE TREATMENT AND METHOD FOR TREATING A FABRIC SITE WITH NEGATIVE PRESSURE |
WO2018226624A1 (en) | 2017-06-07 | 2018-12-13 | Kci Licensing, Inc. | Composite dressings for improved granulation and reduced maceration with negative-pressure treatment |
CA3065529A1 (en) | 2017-06-07 | 2018-12-13 | Kci Licensing, Inc. | Composite dressings for improved granulation reduced maceration with negative-pressure treatment |
RU2019142320A (en) | 2017-06-07 | 2021-07-09 | Кейсиай ЛАЙСЕНСИНГ, ИНК. | Peelable and In-Place Negative Pressure Therapy Dressing |
CA3065379A1 (en) | 2017-06-07 | 2018-12-13 | Kci Licensing, Inc. | Systems, apparatuses, and methods for negative-pressure treatment with reduced tissue in-growth |
EP3634337B1 (en) | 2017-06-07 | 2023-05-24 | 3M Innovative Properties Company | Methods for manufacturing and assembling dual material tissue interface for negative-pressure therapy |
WO2018226592A1 (en) | 2017-06-07 | 2018-12-13 | Kci Usa, Inc. | Wound dressing with odor absorption and increased moisture vapor transmission |
WO2018226705A1 (en) | 2017-06-07 | 2018-12-13 | Kci Licensing, Inc. | Composite dressings for improved granulation and reduced maceration with negative-pressure treatment |
US20200085629A1 (en) | 2017-06-07 | 2020-03-19 | Kci Licensing, Inc. | Composite dressings with even expansion profiles for treatment of wounds using negative-pressure treatment |
CN110944607A (en) | 2017-06-07 | 2020-03-31 | 凯希特许有限公司 | Method of manufacturing and assembling a bi-material tissue interface for negative pressure therapy |
US11471584B2 (en) | 2017-06-07 | 2022-10-18 | Kci Licensing, Inc. | Composite dressings for improved granulation and reduced maceration with negative-pressure treatment |
KR20200016931A (en) | 2017-06-07 | 2020-02-17 | 케이씨아이 라이센싱 인코포레이티드 | Dressing material that can be detached and attached for sticky exudates and drip infusions |
EP3634521A1 (en) | 2017-06-08 | 2020-04-15 | KCI Licensing, Inc. | Negative-pressure therapy with oxygen |
EP3412319A1 (en) | 2017-06-09 | 2018-12-12 | Mölnlycke Health Care AB | Foam in wound treatment |
EP3634327B1 (en) | 2017-06-09 | 2023-08-30 | 3M Innovative Properties Company | Granulating dressing for low exuding chronic wounds |
WO2018231825A1 (en) | 2017-06-12 | 2018-12-20 | Kci Licensing, Inc. | Foamed and textured sintered polymer wound filler |
JP7179022B2 (en) | 2017-06-13 | 2022-11-28 | スミス アンド ネフュー ピーエルシー | Wound closure device and method of use |
EP3638167B1 (en) | 2017-06-14 | 2023-11-01 | T.J. Smith and Nephew, Limited | Negative pressure wound therapy apparatus |
WO2018231874A1 (en) | 2017-06-14 | 2018-12-20 | Smith & Nephew, Inc. | Control of wound closure and fluid removal management in wound therapy |
CA3063859A1 (en) | 2017-06-14 | 2018-12-20 | Smith & Nephew, Inc. | Fluid removal management and control of wound closure in wound therapy |
EP3641714A1 (en) | 2017-06-19 | 2020-04-29 | KCI USA, Inc. | Wound dressing with saturation indicator |
JP7189159B2 (en) | 2017-06-23 | 2022-12-13 | スミス アンド ネフュー ピーエルシー | Sensor placement for sensor-enabled wound monitoring or therapy |
US20200214898A1 (en) | 2017-06-26 | 2020-07-09 | Kci Usa, Inc. | Absorbent wound dressing that incorporates a novel wound fluid indicating system |
US10751212B2 (en) | 2017-06-26 | 2020-08-25 | Maryam Raza | Multilayer dressing device and method for preventing and treating pressure ulcers and chronic wounds |
DE102017006025A1 (en) | 2017-06-27 | 2018-12-27 | Carl Freudenberg Kg | Hydrogel-forming multicomponent fiber |
US11554051B2 (en) | 2017-06-30 | 2023-01-17 | T.J. Smith And Nephew, Limited | Negative pressure wound therapy apparatus |
US20200121510A1 (en) | 2017-07-07 | 2020-04-23 | Smith & Nephew Plc | Wound therapy system and dressing for delivering oxygen to a wound |
FR3068975B1 (en) | 2017-07-12 | 2020-07-17 | Urgo Recherche Innovation Et Developpement | COMPOSITION FOR INTERFACE DRESSING |
FR3068974B1 (en) | 2017-07-12 | 2019-08-02 | Urgo Recherche Innovation Et Developpement | DRESSING FOR THE CONTROLLED AND PROLONGED DELIVERY OF ASSETS |
GB201711181D0 (en) | 2017-07-12 | 2017-08-23 | Smith & Nephew | Polymer foam material, device and use |
GB201711179D0 (en) | 2017-07-12 | 2017-08-23 | Smith & Nephew | Wound care materials, devices and uses |
GB201711183D0 (en) | 2017-07-12 | 2017-08-23 | Smith & Nephew | Antimicrobial or wound care materials, devices and uses |
CN110621354B (en) | 2017-07-21 | 2023-03-10 | 矿物快速护理有限公司 | Novel wound dressing for hemostasis |
GB201804502D0 (en) | 2018-03-21 | 2018-05-02 | Smith & Nephew | Biocompatible encapsulation and component stress relief for sensor enabled negative pressure wound therapy dressings |
CN110997019A (en) | 2017-07-26 | 2020-04-10 | 优瑞株式会社 | Wound dressing comprising hyaluronic acid-calcium and polylysine and method for producing same |
GB201712165D0 (en) | 2017-07-28 | 2017-09-13 | Smith & Nephew | Wound dressing and method of manufacture |
US10780201B2 (en) | 2017-07-29 | 2020-09-22 | Edward D. Lin | Control apparatus and related methods for wound therapy delivery |
US10729826B2 (en) | 2017-07-29 | 2020-08-04 | Edward D. Lin | Wound cover apparatus and related methods of use |
WO2019027933A1 (en) | 2017-07-31 | 2019-02-07 | Kci Usa, Inc. | Bioresorbable dressing with structural support |
JP2020529246A (en) | 2017-08-02 | 2020-10-08 | ケーシーアイ ライセンシング インコーポレイテッド | Systems and methods for wound wound resection |
WO2019027731A1 (en) | 2017-08-02 | 2019-02-07 | Kci Licensing, Inc. | Multi-layer compartment dressing and negative-pressure treatment method |
EP3664756B1 (en) | 2017-08-07 | 2024-01-24 | Smith & Nephew plc | Wound closure device with protective layer |
DE102017117828A1 (en) | 2017-08-07 | 2019-02-07 | Ivf Hartmann Ag | Bandage, in particular compression bandage |
EP3666300B1 (en) | 2017-08-09 | 2023-02-15 | Toray Industries, Inc. | Medical device and method for manufacturing same |
WO2019030384A2 (en) | 2017-08-10 | 2019-02-14 | Smith & Nephew Plc | Positioning of sensors for sensor enabled wound monitoring or therapy |
US20200197580A1 (en) | 2017-08-22 | 2020-06-25 | Kci Licensing, Inc. | In-line wound fluid sampling systems and methods for use with negative pressure wound therapy |
GB201713511D0 (en) | 2017-08-23 | 2017-10-04 | Scapa Uk Ltd | Wound dressing |
WO2019040656A1 (en) | 2017-08-23 | 2019-02-28 | Cor Medical Ventures LLC | Post-operative surgical site wound treatment and method for device removal |
US11246756B2 (en) | 2017-08-24 | 2022-02-15 | The United States Of America, As Represented By The Secretary Of Agriculture | Healthcare textiles |
GB2565823A (en) | 2017-08-24 | 2019-02-27 | Xiros Ltd | Psyllium based moisture absorbent material |
EP3672655B1 (en) | 2017-08-24 | 2022-01-19 | KCI USA, Inc. | Biomaterial and methods of making and using said biomaterial |
WO2019050855A1 (en) | 2017-09-05 | 2019-03-14 | Kci Licensing, Inc. | Systems and methods for mitigating premature light deactivation of light deactivated adhesive drapes |
JP2020532378A (en) | 2017-09-05 | 2020-11-12 | ケーシーアイ ライセンシング インコーポレイテッド | Systems and methods that use a filtering layer to reduce the premature photoinactivation of photoinactivated adhesive drapes |
CN111093477B (en) | 2017-09-10 | 2023-09-12 | 史密夫及内修公开有限公司 | System and method for inspecting packaging and components in sensor-equipped wound dressings |
AU2018331954B2 (en) | 2017-09-13 | 2024-07-04 | Smith & Nephew Plc | Negative pressure wound treatment apparatuses and methods with integrated electronics |
GB201718070D0 (en) | 2017-11-01 | 2017-12-13 | Smith & Nephew | Negative pressure wound treatment apparatuses and methods with integrated electronics |
WO2019055176A1 (en) | 2017-09-14 | 2019-03-21 | Kci Licensing, Inc. | Oxygen therapy with fluid removal |
EP3681545A4 (en) | 2017-09-15 | 2020-11-11 | Bard Access Systems, Inc. | Antimicrobial dressing with liner for a medical device |
US12004926B2 (en) | 2017-09-18 | 2024-06-11 | Kci Licensing, Inc. | Wound dressings and systems with remote oxygen generation for topical wound therapy and related methods |
US11547611B2 (en) | 2017-09-22 | 2023-01-10 | Kci Licensing, Inc. | Wound dressings and systems with high-flow therapeutic gas sources for topical wound therapy and related methods |
IL254636A0 (en) | 2017-09-24 | 2017-11-30 | Reddress Ltd | Assembly and method for the preparation of a wound dressing |
IL254644B (en) | 2017-09-24 | 2021-06-30 | Reddress Ltd | Wound dressing device, assembly and method |
GB2566951A (en) | 2017-09-27 | 2019-04-03 | Brightwake Ltd | Compositions for wound treatment |
EP3687467B1 (en) | 2017-09-29 | 2022-02-23 | 3M Innovative Properties Company | Dressing exhibiting low tissue ingrowth and negative-pressure treatment method |
US20210393203A1 (en) | 2017-10-09 | 2021-12-23 | 3M Innovative Properties Company | Securement dressing with conformal border |
GB201716986D0 (en) | 2017-10-16 | 2017-11-29 | Matoke Holdings Ltd | Antimicrobial compositions |
ES2911647T3 (en) | 2017-10-20 | 2022-05-20 | Safeguard Medical Tech Limited | Composition |
CA3082020A1 (en) | 2017-10-23 | 2019-05-02 | Kci Licensing, Inc. | Low profile distribution components for wound therapy |
WO2019083607A1 (en) | 2017-10-23 | 2019-05-02 | Kci Licensing, Inc. | High-density evaporative bridge dressing |
AU2018355151B2 (en) | 2017-10-23 | 2024-09-19 | Solventum Intellectual Properties Company | Area management of tissue sites on articulating joints |
WO2019083726A1 (en) | 2017-10-23 | 2019-05-02 | Kci Licensing, Inc. | Wound dressing for use with anti-bacterial material |
US11432967B2 (en) | 2017-10-23 | 2022-09-06 | Kci Licensing, Inc. | Fluid bridge for simultaneous application of negative pressure to multiple tissue sites |
CN111447901A (en) | 2017-10-24 | 2020-07-24 | 凯希特许有限公司 | Debridement wound dressing and system for using same |
CN111278942A (en) | 2017-10-26 | 2020-06-12 | 3M创新有限公司 | Compositions and methods and articles comprising silicone-based binders and cellulose nanocrystals |
US11969317B2 (en) | 2017-10-26 | 2024-04-30 | 3M Innovative Properties Company | Wound dressing with welded elastic structure |
EP3700597B8 (en) | 2017-10-26 | 2023-11-15 | 3M Innovative Properties Company | Manifolding apparatus |
CA3078733C (en) | 2017-10-27 | 2023-02-21 | Kci Licensing, Inc. | Contoured foam dressing shaped for providing negative pressure to incisions in the breast |
KR102566942B1 (en) | 2017-10-27 | 2023-08-14 | 주식회사 엘지화학 | Preparation method of super absorbent polymer |
WO2019089118A1 (en) | 2017-10-30 | 2019-05-09 | Kci Licensing, Inc. | Systems, apparatuses, and methods for negative-pressure treatment with pressure delivery indication |
WO2019089856A1 (en) | 2017-10-31 | 2019-05-09 | InMEDBio, LLC | Absorbent, breathable and pathogen blocking/killing wound care dressing and fabrication thereof |
GB201718014D0 (en) | 2017-11-01 | 2017-12-13 | Smith & Nephew | Dressing for negative pressure wound therapy with filter |
WO2019086332A1 (en) | 2017-11-01 | 2019-05-09 | Smith & Nephew Plc | Negative pressure wound treatment apparatuses and methods with integrated electronics |
GB201718054D0 (en) | 2017-11-01 | 2017-12-13 | Smith & Nephew | Sterilization of integrated negative pressure wound treatment apparatuses and sterilization methods |
IL255404B (en) | 2017-11-02 | 2018-10-31 | Technion Res & Dev Foundation | Hipe-templated zwitterionic hydrogels, process of preparation and uses thereof |
WO2019089522A1 (en) | 2017-11-02 | 2019-05-09 | Systagenix Wound Management, Limited | Wound dressing with humidity colorimeter sensor |
US11771599B2 (en) | 2017-11-03 | 2023-10-03 | Kci Licensing, Inc. | Extended wear-time dressing |
EP3703636A1 (en) | 2017-11-03 | 2020-09-09 | Systagenix Wound Management, Limited | Nutrient-enriched dressing |
GB2568101B (en) | 2017-11-06 | 2022-09-07 | Brightwake Ltd | Antimicrobial dressing |
EP3706689A4 (en) | 2017-11-08 | 2021-12-01 | University Of Massachusetts | Post-operative hybrid dressing to optimize skin-grafting procedures in reconstructive surgery |
US20210177661A1 (en) | 2017-11-09 | 2021-06-17 | Kci Licensing, Inc. | Multi-module dressing and therapy methods |
WO2019094923A1 (en) | 2017-11-13 | 2019-05-16 | Kci Licensing, Inc. | Light-responsive pressure sensitive adhesives for wound dressings |
CN107899061A (en) | 2017-11-13 | 2018-04-13 | 广东泰宝医疗科技股份有限公司 | A kind of alginates wound repair dressing and preparation method thereof |
US11559438B2 (en) | 2017-11-15 | 2023-01-24 | Smith & Nephew Plc | Integrated sensor enabled wound monitoring and/or therapy dressings and systems |
US20200289727A1 (en) | 2017-12-06 | 2020-09-17 | Kci Licensing, Inc. | Wound dressing with negative pressure retaining valve |
US11839527B2 (en) | 2017-12-06 | 2023-12-12 | Cornell University | Manually-operated negative pressure wound therapy (NPWT) bandage with improved pump efficiency, automatic pressure indicator and automatic pressure limiter |
AU2018384069B2 (en) | 2017-12-11 | 2022-12-08 | Animal Ethics Pty Ltd | Wound dressing |
EP3498242A1 (en) | 2017-12-15 | 2019-06-19 | Mölnlycke Health Care AB | Medical dressing |
IL256405A (en) | 2017-12-19 | 2018-01-31 | Omrix Biopharmaceuticals Ltd | Wound dressing and a method for producing the same |
WO2019125962A1 (en) | 2017-12-20 | 2019-06-27 | Kci Licensing, Inc. | Wound dressing for the harvesting of superficial epidermal grafts |
US20200337904A1 (en) | 2017-12-20 | 2020-10-29 | Systagenix Wound Management, Limited | Dressing including dehydrated placental tissue for wound healing |
DE102017130893A1 (en) | 2017-12-21 | 2019-06-27 | Paul Hartmann Ag | pH regulating wound dressing |
WO2019134169A1 (en) | 2018-01-08 | 2019-07-11 | 万绵水 | Connecting device for wound protection dressing, and wound protection dressing |
US11877912B2 (en) | 2018-01-09 | 2024-01-23 | 3M Innovative Properties Company | Systems and methods for coupling a wearable therapy system to a dressing |
CN112040915B (en) | 2018-03-26 | 2022-04-01 | 帝皇工业有限公司 | Multi-lumen bridge for negative pressure wound therapy system |
US20190298882A1 (en) | 2018-03-27 | 2019-10-03 | Kevin M. Nelson | Hydrogel bandage |
US20210077671A1 (en) | 2018-03-29 | 2021-03-18 | Kci Licensing, Inc. | Wound therapy system with wound volume estimation |
EP3773383A1 (en) | 2018-03-30 | 2021-02-17 | 3M Innovative Properties Company | An absorbent dressing incorporating ph wound condition indication |
GB201805584D0 (en) | 2018-04-05 | 2018-05-23 | Smith & Nephew | Negative pressure wound treatment apparatuses and methods with integrated electronics |
GB2572642B (en) | 2018-04-06 | 2021-03-31 | Pellis Care Ltd | Treatment of diabetic foot ulcers |
US11040127B2 (en) | 2018-04-09 | 2021-06-22 | Kci Licensing, Inc. | Abdominal dressing with mechanism for fascial closure |
US20210379273A1 (en) | 2018-04-10 | 2021-12-09 | Kci Licensing, Inc. | Bridge dressing with fluid management |
BR102018007306A2 (en) | 2018-04-11 | 2019-10-29 | Maria Cristina De Paula Mesquita | specific shape adhesive tape / plate making process / silicone dressing |
WO2019199596A1 (en) | 2018-04-12 | 2019-10-17 | Kci Licensing, Inc. | Cutting template for a negative pressure wound therapy drape |
WO2019199849A1 (en) | 2018-04-13 | 2019-10-17 | Kci Licensing, Inc. | Dressing bolster with area pressure indicator |
WO2019200035A1 (en) | 2018-04-13 | 2019-10-17 | Kci Licensing, Inc. | Npwt system with selectively controllable airflow |
WO2019199798A1 (en) | 2018-04-13 | 2019-10-17 | Kci Licensing, Inc. | Compression strain and negative pressure delivery indicator for a wound dressing |
JP7346443B2 (en) | 2018-04-13 | 2023-09-19 | ケーシーアイ ライセンシング インコーポレイテッド | Method for dynamically measuring load and patient limb movement in negative pressure closed incision dressings |
EP3787705B1 (en) | 2018-05-03 | 2023-07-19 | KCI Licensing, Inc. | Negative pressure wound therapy system with detection of full absorbant dressing |
JP2021522967A (en) | 2018-05-04 | 2021-09-02 | ドライ シー エルエルシー | Liquid detection article and its manufacturing method |
KR20210008351A (en) | 2018-05-08 | 2021-01-21 | 피디아 파마슈티치 에스.피.에이. | Wound dressing to treat damaged skin |
EP3569261B1 (en) | 2018-05-14 | 2024-04-03 | Paul Hartmann AG | Functional wound dressing |
EP3569210B1 (en) | 2018-05-15 | 2022-04-27 | The Procter & Gamble Company | Disposable absorbent articles |
US10898606B2 (en) | 2018-05-15 | 2021-01-26 | Legacy Research and Development Group, LLC | Self-fusing low density silicone |
US11253400B2 (en) | 2018-05-16 | 2022-02-22 | Midwest Training and Development Services, LLC | Negative pressure wound apposition dressing system |
US20190351095A1 (en) | 2018-05-21 | 2019-11-21 | Milliken & Company | Wound care device having fluid transfer and adhesive properties |
US20190351094A1 (en) | 2018-05-21 | 2019-11-21 | Milliken & Company | Wound care device having fluid transfer and adhesive properties |
FR3082123B1 (en) | 2018-06-07 | 2020-10-16 | Urgo Rech Innovation Et Developpement | CELLULARIZED DRESSING AND ITS MANUFACTURING PROCESS |
NL2021186B1 (en) | 2018-06-26 | 2020-01-06 | Icap Holding B V | Intelligent cap for skin tissue treatment |
TWI693929B (en) | 2018-06-27 | 2020-05-21 | 南六企業股份有限公司 | Antibacterial wound dressing |
WO2020005536A1 (en) | 2018-06-27 | 2020-01-02 | Kci Licensing, Inc. | Wound dressing for wound volume estimation |
US11701264B2 (en) | 2018-06-27 | 2023-07-18 | Kci Licensing, Inc. | Wound therapy system with wound volume estimation using geometric approximation |
US11896462B2 (en) | 2018-06-28 | 2024-02-13 | 3M Innovative Properties Company | Highly conformable wound dressing |
WO2020005577A1 (en) | 2018-06-28 | 2020-01-02 | Kci Licensing, Inc. | Distributed negative pressure wound therapy system incorporating an absorbent dressing and piezo-electric pump |
WO2020003067A2 (en) | 2018-06-28 | 2020-01-02 | Systagenix Wound Management, Limited | Multilayer absorbent dressing construction |
US20200000643A1 (en) | 2018-06-28 | 2020-01-02 | Kci Licensing, Inc. | Long-Duration, Deep Wound Filler With Means To Prevent Granulation In-Growth |
WO2020005344A1 (en) | 2018-06-28 | 2020-01-02 | Kci Licensing, Inc. | Release liner with edge protection |
US20200000640A1 (en) | 2018-06-29 | 2020-01-02 | Milliken & Company | Multi-Layer Wound Care Device Having Absorption and Fluid Transfer Properties |
US20210178012A1 (en) | 2018-07-04 | 2021-06-17 | Coloplast A/S | Foam wound dressing comprising an antiseptic |
EP3820417A1 (en) | 2018-07-12 | 2021-05-19 | KCI Licensing, Inc. | Abdominal dressing with user selection of fascial closure force profile |
GB201811449D0 (en) | 2018-07-12 | 2018-08-29 | Smith & Nephew | Apparatuses and methods for negative pressure wound therapy |
WO2020014310A1 (en) | 2018-07-13 | 2020-01-16 | Kci Licensing, Inc. | Advanced wound dressing with compression and increased total fluid handling |
WO2020018300A1 (en) | 2018-07-16 | 2020-01-23 | Kci Licensing, Inc. | Fluid instillation apparatus for use with negative-pressure system incorporating wireless therapy monitoring |
US11383019B2 (en) | 2018-07-18 | 2022-07-12 | Kci Licensing, Inc. | Wound view dressing and customization kit |
WO2020026061A1 (en) | 2018-07-30 | 2020-02-06 | 3M Innovative Properties Company | Antimicrobial foam articles and method of making the same |
US11534343B2 (en) | 2018-07-31 | 2022-12-27 | Kci Licensing, Inc. | Devices and methods for preventing localized pressure points in distribution components for tissue therapy |
US20200038252A1 (en) | 2018-07-31 | 2020-02-06 | Joseph Spiro | Tri-layered wound dressing and method therefor |
JP2021531899A (en) | 2018-08-01 | 2021-11-25 | ケーシーアイ ライセンシング インコーポレイテッド | Soft tissue treatment using negative pressure |
WO2020026144A1 (en) | 2018-08-01 | 2020-02-06 | Systagenix Wound Management, Limited | Dressing packaging with controlled hydration of fluid-activated dressing |
US20200038251A1 (en) | 2018-08-03 | 2020-02-06 | Kci Licensing, Inc. | Flexible and conformable wound dressing with enhanced fluid absorption capability |
US11253401B2 (en) | 2018-08-03 | 2022-02-22 | Kci Licensing, Inc. | Wound therapy system with wound volume estimation |
US20210161725A1 (en) | 2018-08-10 | 2021-06-03 | Kci Licensing, Inc. | Wound dressing system for management of fluids in a wound and methods for manufacturing same |
US20200046876A1 (en) | 2018-08-13 | 2020-02-13 | Chuang Sheng Medicine Equipment Co. Ltd. | Hydrogel surgical dressing product having a multi-dimensional flexible hydrophilic structure-linkage composite |
WO2020036785A1 (en) | 2018-08-13 | 2020-02-20 | Kci Licensing, Inc. | Disruptive dressing for use with negative pressure and fluid instillation |
WO2020035811A1 (en) | 2018-08-17 | 2020-02-20 | 3M Innovative Properties Company | Wound dressing system |
US11938236B2 (en) | 2018-08-17 | 2024-03-26 | Seoul Viosys Co., Ltd. | Medical dressing |
WO2020040917A1 (en) | 2018-08-21 | 2020-02-27 | Kci Licensing, Inc. | Dressing and system with improved total fluid handling |
EP3840794B1 (en) | 2018-08-21 | 2023-10-11 | 3M Innovative Properties Company | System for utilizing pressure decay to determine available fluid capacity in a negative pressure dressing |
US11395918B2 (en) | 2018-08-24 | 2022-07-26 | United States Government As Represented By The Department Of Veterans Affairs | Devices, and systems for remotely monitoring and treating wounds or wound infections |
US11278454B2 (en) | 2018-08-24 | 2022-03-22 | Kci Licensing, Inc. | Methods of managing moisture when using a low profile wound connection conduit |
WO2020043665A1 (en) | 2018-08-27 | 2020-03-05 | Claudia Eder | Antiseptic gel |
AU2019331721B2 (en) | 2018-08-27 | 2022-04-21 | Advamedica Inc. | Composite dressings, manufacturing methods and applications thereof |
US11752039B2 (en) | 2018-08-28 | 2023-09-12 | Systagenix Wound Management, Limited | Dressings for reduced tissue ingrowth |
US11007083B2 (en) | 2018-08-28 | 2021-05-18 | Aatru Medical, LLC | Dressing |
WO2020046589A1 (en) | 2018-08-30 | 2020-03-05 | Kci Licensing, Inc. | Electro-mechanical pump for negative-pressure treatment |
WO2020047255A1 (en) | 2018-08-31 | 2020-03-05 | Kci Licensing, Inc. | Cooling dressing for improved comfort |
CN112584802B (en) | 2018-09-04 | 2023-03-17 | 凯希特许有限公司 | Wound treatment device and kit |
CN113164286B (en) | 2018-09-04 | 2023-08-22 | 罗曼-劳氏股份有限公司 | wound cleaning device |
US11471335B2 (en) | 2018-09-05 | 2022-10-18 | University Of South Carolina | Gel-within-gel wound dressing |
WO2020051273A1 (en) | 2018-09-05 | 2020-03-12 | Kci Licensing, Inc. | Systems and methods for scheduling and controlling wound therapy |
US20220111138A1 (en) | 2018-09-12 | 2022-04-14 | Kci Licensing, Inc. | Systems, apparatuses, and methods for negative-pressure treatment with reduced tissue in-growth |
CN112912117A (en) | 2018-09-12 | 2021-06-04 | 凯希特许有限公司 | Wound treatment system using instillation therapy and dynamic pressure control |
WO2020055945A1 (en) | 2018-09-12 | 2020-03-19 | Kci Licensing, Inc. | Negative pressure wound therapy systems and methods to indicate total fluid handling |
CN112912042A (en) | 2018-09-14 | 2021-06-04 | 凯希特许有限公司 | Differential collapse wound dressing |
EP3852828B1 (en) | 2018-09-17 | 2024-04-10 | Solventum Intellectual Properties Company | Fluid ingress protection for npwt device |
WO2020060918A1 (en) | 2018-09-17 | 2020-03-26 | Kci Licensing, Inc. | Absorbent negative pressure dressing |
US11305050B2 (en) | 2018-09-19 | 2022-04-19 | Deroyal Industries, Inc. | Connector with valve for negative pressure wound therapy system |
EP3852705A1 (en) | 2018-09-20 | 2021-07-28 | KCI Licensing, Inc. | Super-absorbent, low trauma, advanced wound dressing |
US20220047771A1 (en) | 2018-09-25 | 2022-02-17 | Systagenix Wound Management, Limited | Wound dressing compositions and uses thereof |
DE102018007692A1 (en) | 2018-09-30 | 2020-04-02 | Alexander Folwarzny | Wound dressing |
US11432965B2 (en) | 2018-10-05 | 2022-09-06 | Deborah Kantor | Medical bandage for the head, a limb or a stump |
EP3632476A1 (en) | 2018-10-05 | 2020-04-08 | John J. Ryan (Sealing Products) Limited | Wound contact surface and method of manufacture |
US11266538B2 (en) | 2018-10-07 | 2022-03-08 | Michael David Francis | Adhesive wound dressing |
EP3636233B1 (en) | 2018-10-12 | 2023-06-07 | IVF Hartmann AG | Wet wound dressing having an adhesive edge |
EP3866871A1 (en) | 2018-10-15 | 2021-08-25 | KCI Licensing, Inc. | Micro balloon-on-tube wound filler |
FR3087126A1 (en) | 2018-10-16 | 2020-04-17 | Jean Francois Van Cleef | COMPOSITE WOUND MOLDING PROTECTION DEVICE |
US20200121509A1 (en) | 2018-10-17 | 2020-04-23 | Kci Licensing, Inc. | Peel and place dressing having a closed-cell contact layer |
EP3866741A1 (en) | 2018-10-17 | 2021-08-25 | KCI Licensing, Inc. | Systems, apparatuses, and methods for negative-pressure treatment with reduce tissue in-growth |
GB2611267B (en) | 2018-10-18 | 2023-06-28 | Smith & Nephew | Tissue treatment device |
GB201817052D0 (en) | 2018-10-19 | 2018-12-05 | Smith & Nephew | Tissue treatment device |
US11839528B2 (en) | 2018-10-22 | 2023-12-12 | Medline Industries, Lp | Drypad with rapid absorption and liquid removal |
US20200129339A1 (en) | 2018-10-24 | 2020-04-30 | Hydrofera, Llc | Sterilization of medical devices with enhanced antimicrobial properties |
EP3643330A1 (en) | 2018-10-24 | 2020-04-29 | Paul Hartmann AG | Ph-triggered buffered wound dressing |
EP3643331A1 (en) | 2018-10-24 | 2020-04-29 | Paul Hartmann AG | Ph-triggered therapeutic wound dressing |
EP3643328A1 (en) | 2018-10-24 | 2020-04-29 | Paul Hartmann AG | Ph-triggered diagnostic wound dressing |
WO2020092598A1 (en) | 2018-10-30 | 2020-05-07 | Kci Licensing, Inc. | Ease of use dressing with integrated pouch and release liner |
MX2021005161A (en) | 2018-11-02 | 2021-10-13 | Covalon Tech Inc | Foam compositions, foam matrices and methods. |
JP2022506830A (en) | 2018-11-08 | 2022-01-17 | ケーシーアイ ライセンシング インコーポレイテッド | Dressing with a protruding layer that allows cleaning of wound bed macrodeformations |
WO2020097529A1 (en) | 2018-11-08 | 2020-05-14 | Kci Licensing, Inc. | Wound dressing with semi-rigid support to increase disruption using perforated dressing and negative pressure wound therapy |
US20200146899A1 (en) | 2018-11-09 | 2020-05-14 | Kci Licensing, Inc. | Hybrid adhesive tissue cover |
CN113507907B (en) | 2018-11-13 | 2023-04-21 | 3M创新知识产权公司 | Thin dispensing member for wound treatment |
GB2579211A (en) | 2018-11-23 | 2020-06-17 | Brightwake Ltd | Medical tube |
GB2579368B (en) | 2018-11-29 | 2022-11-09 | Nexa Medical Ltd | Wound-dressing conditioning device |
US20200179300A1 (en) | 2018-12-10 | 2020-06-11 | Joseph Urban | Topical Formulation Cures and Heals a Variety of Skin Conditions Including Ulcers, Decubitus Ulcers, Cancer, Abrasions and other Conditions and also accelerates the curing and healing of those Conditions |
GB2579800B (en) | 2018-12-13 | 2021-11-03 | Adv Med Solutions Ltd | Resilient wound dressing |
GB2579790B (en) | 2018-12-13 | 2022-10-05 | Adv Med Solutions Ltd | Ribbon wound dressing |
WO2020124038A1 (en) | 2018-12-13 | 2020-06-18 | University Of Massachusetts | Negative pressure wound closure devices and methods |
HUE064633T2 (en) | 2018-12-21 | 2024-04-28 | Hartmann Paul Ag | Superabsorbent wound dressing with silicone wound contact layer |
EP3669838A1 (en) | 2018-12-21 | 2020-06-24 | Paul Hartmann S.A. | Array of absorbent dressings for the treatment of wounds |
EP3669844B1 (en) | 2018-12-21 | 2024-07-03 | Paul Hartmann AG | Superabsorbent wound dressing with silicone wound contact layer |
EP3902882A1 (en) | 2018-12-27 | 2021-11-03 | 3M Innovative Properties Company | Hot melt processable (meth)acrylate-based medical adhesives |
GB201900015D0 (en) | 2019-01-02 | 2019-02-13 | Smith & Nephew | Negative pressure wound therapy apparatus |
WO2020142284A1 (en) | 2019-01-03 | 2020-07-09 | Kci Licensing, Inc. | Superabsorbent laminate dressing |
GB201900407D0 (en) | 2019-01-11 | 2019-02-27 | Smith & Nephew | Method of manufacturing a component for a wound dressing |
CN109481731B (en) | 2019-01-23 | 2020-03-27 | 中南大学 | Nano oxide/kaolin composite hemostatic and antibacterial material, hemostatic and healing-promoting dressing and preparation method thereof |
WO2020154175A1 (en) | 2019-01-24 | 2020-07-30 | Kci Licensing, Inc. | Variable density dressing |
JP2022517867A (en) | 2019-01-28 | 2022-03-10 | ハイプロテック・インコーポレイテッド | Antibacterial composition with anticoagulant, immunomodulatory, and tissue regenerative properties |
JP7367034B2 (en) | 2019-01-28 | 2023-10-23 | ケーシーアイ ライセンシング インコーポレイテッド | Tearable dressing structure |
WO2020159823A1 (en) | 2019-01-29 | 2020-08-06 | Kci Licensing, Inc. | Removable and replaceable dressing interface for a negative-pressure therapy system |
EP3917472A1 (en) | 2019-01-29 | 2021-12-08 | KCI Licensing, Inc. | Absorbent dressing with indicator and mechanical decoupling of expansion forces |
WO2020159752A1 (en) | 2019-02-01 | 2020-08-06 | Kci Licensing, Inc. | Drape strip having selectable adhesive |
CN113365588B (en) | 2019-02-01 | 2022-08-30 | 凯希特许有限公司 | Partially transparent wound dressing |
EP3917476A1 (en) | 2019-02-01 | 2021-12-08 | KCI Licensing, Inc. | Abdominal negative pressure therapy dressing with remote wound sensing capability |
TWM578166U (en) | 2019-02-01 | 2019-05-21 | 嬌朋生技股份有限公司 | Negative pressure wound dressing |
CN113168315A (en) | 2019-02-01 | 2021-07-23 | 惠普发展公司,有限责任合伙企业 | Upgrading based on analysis from multiple sources |
WO2020161086A1 (en) | 2019-02-04 | 2020-08-13 | T.J.Smith And Nephew,Limited | Wound contact layer and dressing for iodine delivery |
WO2020162953A1 (en) | 2019-02-06 | 2020-08-13 | Kci Licensing, Inc. | Wound therapy system with internal alternating orifice |
CN113382700A (en) | 2019-02-07 | 2021-09-10 | 凯希特许有限公司 | Contoured foam dressing shaped to provide negative pressure to an incision in a shoulder |
IT201900002767A1 (en) | 2019-02-26 | 2020-08-26 | Univ Bologna Alma Mater Studiorum | MEDICAL DEVICE FOR THE TREATMENT OF SKIN DISEASES AND RELATIVE METHOD OF IMPLEMENTATION |
US11529504B2 (en) | 2019-02-27 | 2022-12-20 | Jeffrey Wayne Hegg | Dynamic gas-flow wound dressing assembly and method for enhancing the effect of generated gas flow across a wound |
CN113507909A (en) | 2019-02-27 | 2021-10-15 | 希丝塔杰尼斯创伤护理有限公司 | Antimicrobial dressings, dressing components, and methods |
EP3930770A1 (en) | 2019-02-28 | 2022-01-05 | Speed Care Mineral GmbH | Paste for marking textile fabrics and/or other products incapable of x-ray contrast |
US20220145036A1 (en) | 2019-02-28 | 2022-05-12 | Covestro Intellectual Property Gmbh & Co. Kg | Thermoplastic foam prepared from two special polyurethane dispersions |
-
2010
- 2010-11-30 GB GBGB1020236.4A patent/GB201020236D0/en not_active Ceased
-
2011
- 2011-11-30 CA CA2819303A patent/CA2819303C/en active Active
- 2011-11-30 NZ NZ612573A patent/NZ612573A/en not_active IP Right Cessation
- 2011-11-30 RU RU2013129866A patent/RU2650803C2/en active
- 2011-11-30 PT PT118059054T patent/PT2646566T/en unknown
- 2011-11-30 EP EP11805905.4A patent/EP2646566B1/en active Active
- 2011-11-30 CN CN201810229737.9A patent/CN108330157A/en active Pending
- 2011-11-30 DK DK11805905.4T patent/DK2646566T3/en active
- 2011-11-30 AU AU2011334682A patent/AU2011334682B2/en not_active Ceased
- 2011-11-30 ES ES11805905.4T patent/ES2609612T3/en active Active
- 2011-11-30 SI SI201131071A patent/SI2646566T1/en unknown
- 2011-11-30 CN CN2011800663752A patent/CN103328651A/en active Pending
- 2011-11-30 US US13/990,755 patent/US20140161728A1/en not_active Abandoned
- 2011-11-30 BR BR112013013662A patent/BR112013013662A2/en not_active Application Discontinuation
- 2011-11-30 PL PL11805905T patent/PL2646566T3/en unknown
- 2011-11-30 HU HUE11805905A patent/HUE032884T2/en unknown
- 2011-11-30 JP JP2013541415A patent/JP2014500495A/en active Pending
- 2011-11-30 MX MX2013006090A patent/MX344623B/en active IP Right Grant
- 2011-11-30 LT LTEP11805905.4T patent/LT2646566T/en unknown
- 2011-11-30 WO PCT/GB2011/001665 patent/WO2012072980A1/en active Application Filing
-
2016
- 2016-10-14 JP JP2016202835A patent/JP2017062238A/en active Pending
-
2017
- 2017-02-17 AU AU2017201084A patent/AU2017201084B2/en not_active Ceased
-
2018
- 2018-07-06 JP JP2018128781A patent/JP2018189655A/en active Pending
-
2019
- 2019-01-25 HK HK19101310.0A patent/HK1258842A1/en unknown
- 2019-03-07 US US16/295,774 patent/US11135315B2/en active Active
-
2021
- 2021-08-10 US US17/398,560 patent/US20210369874A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6555508B1 (en) * | 2000-02-17 | 2003-04-29 | Leonard Paul | Liquid foaming soap compositions |
US20080226724A1 (en) * | 2007-01-19 | 2008-09-18 | Genentech, Inc. | Prevention of hydrogel viscosity loss |
WO2010070292A1 (en) * | 2008-12-20 | 2010-06-24 | Convatec Technologies Inc | A composition for use on skin and wound |
Non-Patent Citations (1)
Title |
---|
Capinera et al. Insecticidal activity of photoactive dyes to American and migratory grasshoppers (Orthoptera: Acrididae). 2000 J. Econ. Entomol. 93: 662-666. * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11452291B2 (en) | 2007-05-14 | 2022-09-27 | The Research Foundation for the State University | Induction of a physiological dispersion response in bacterial cells in a biofilm |
US11135315B2 (en) | 2010-11-30 | 2021-10-05 | Convatec Technologies Inc. | Composition for detecting biofilms on viable tissues |
US20170315120A1 (en) * | 2016-05-02 | 2017-11-02 | James M. Rynerson | Methods and devices for detecting biofilms on the eyelid margins |
WO2018157368A1 (en) * | 2017-03-03 | 2018-09-07 | The Procter & Gamble Company | Calcium fluorescent probes to assess oral care composition efficacy in biofilm |
US11541105B2 (en) | 2018-06-01 | 2023-01-03 | The Research Foundation For The State University Of New York | Compositions and methods for disrupting biofilm formation and maintenance |
US11572578B2 (en) * | 2018-07-31 | 2023-02-07 | Saraya Co., Ltd. | Reagent kit for detecting biofilm and method for detecting biofilm |
Also Published As
Publication number | Publication date |
---|---|
CA2819303A1 (en) | 2012-06-07 |
AU2017201084A1 (en) | 2017-03-09 |
HUE032884T2 (en) | 2017-11-28 |
CN108330157A (en) | 2018-07-27 |
BR112013013662A2 (en) | 2016-09-06 |
PL2646566T3 (en) | 2017-06-30 |
WO2012072980A1 (en) | 2012-06-07 |
EP2646566B1 (en) | 2016-10-19 |
PT2646566T (en) | 2017-01-26 |
MX344623B (en) | 2017-01-03 |
CA2819303C (en) | 2022-04-19 |
RU2650803C2 (en) | 2018-04-17 |
AU2011334682B2 (en) | 2016-11-17 |
LT2646566T (en) | 2017-01-25 |
JP2014500495A (en) | 2014-01-09 |
AU2017201084B2 (en) | 2019-04-18 |
HK1258842A1 (en) | 2019-11-22 |
ES2609612T3 (en) | 2017-04-21 |
DK2646566T3 (en) | 2017-01-23 |
JP2017062238A (en) | 2017-03-30 |
EP2646566A1 (en) | 2013-10-09 |
AU2011334682A1 (en) | 2013-07-18 |
US20210369874A1 (en) | 2021-12-02 |
CN103328651A (en) | 2013-09-25 |
SI2646566T1 (en) | 2017-02-28 |
RU2013129866A (en) | 2015-01-10 |
GB201020236D0 (en) | 2011-01-12 |
MX2013006090A (en) | 2013-07-15 |
NZ612573A (en) | 2015-05-29 |
JP2018189655A (en) | 2018-11-29 |
US11135315B2 (en) | 2021-10-05 |
US20190216953A1 (en) | 2019-07-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11135315B2 (en) | Composition for detecting biofilms on viable tissues | |
JP6263465B2 (en) | Composition for use in skin and wounds | |
Cassidy et al. | Drug delivery strategies for photodynamic antimicrobial chemotherapy: from benchtop to clinical practice | |
CN102300587A (en) | Combination Of An Oxidant And A Photoactivator For The Healing Of Wounds | |
Tedesco et al. | Antimicrobial photodynamic therapy (APDT) action based on nanostructured photosensitizers | |
Li et al. | Antimicrobial photodynamic therapy against oral biofilm: influencing factors, mechanisms, and combined actions with other strategies | |
Shiotsu-Ogura et al. | Antimicrobial photodynamic therapy using a plaque disclosing solution on Streptococcus mutans | |
He et al. | Toluidine blue O-induced photoinactivation inhibit the biofilm formation of methicillin-resistant Staphylococcus aureus | |
US20190029994A1 (en) | Biophotonic compositions for the treatment of otitis externa | |
Koker et al. | Real‐time visualization of photochemically induced fluorescence of 8‐halogenated quinolones: Lomefloxacin, clinafloxacin and Bay3118 in live human HaCaT keratinocytes | |
Alves et al. | Effects on Virulence Factors and Mechanisms Involved in Antimicrobial Sonophotodynamic Therapy Mediated by Curcumin | |
Omar | Killing of organisms responsible for wound infections using a light-activated antimicrobial agent | |
小椋有香子 et al. | Antimicrobial photodynamic therapy using a plaque disclosing solution on Streptococcus mutans |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: CONVATEC TECHNOLOGIES INC., NEVADA Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BOWLER, PHILLIP GODFREY;METCALF, DANIEL GARY;PARSONS, DAVID;AND OTHERS;SIGNING DATES FROM 20130607 TO 20130723;REEL/FRAME:035130/0388 |
|
AS | Assignment |
Owner name: WILMINGTON TRUST (LONDON) LIMITED, AS COLLATERAL AGENT, UNITED KINGDOM Free format text: SECURITY AGREEMENT;ASSIGNORS:CONVATEC INC.;CONVATEC TECHNOLOGIES INC.;180 MEDICAL, INC.;AND OTHERS;REEL/FRAME:040552/0227 Effective date: 20161031 Owner name: WILMINGTON TRUST (LONDON) LIMITED, AS COLLATERAL A Free format text: SECURITY AGREEMENT;ASSIGNORS:CONVATEC INC.;CONVATEC TECHNOLOGIES INC.;180 MEDICAL, INC.;AND OTHERS;REEL/FRAME:040552/0227 Effective date: 20161031 |
|
STCV | Information on status: appeal procedure |
Free format text: NOTICE OF APPEAL FILED |
|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |
|
AS | Assignment |
Owner name: CONVATEC TECHNOLOGIES INC., NEVADA Free format text: RELEASE BY SECURED PARTY;ASSIGNOR:WILMINGTON TRUST (LONDON) LIMITED;REEL/FRAME:050831/0001 Effective date: 20191024 |