US20120252109A1 - Reagent cartridge for an assembly for selectively performing a clincial chemical test or an elisa test, use of said reagent cartridge and assembly - Google Patents

Reagent cartridge for an assembly for selectively performing a clincial chemical test or an elisa test, use of said reagent cartridge and assembly Download PDF

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Publication number
US20120252109A1
US20120252109A1 US13/503,276 US201013503276A US2012252109A1 US 20120252109 A1 US20120252109 A1 US 20120252109A1 US 201013503276 A US201013503276 A US 201013503276A US 2012252109 A1 US2012252109 A1 US 2012252109A1
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United States
Prior art keywords
reagent cartridge
reagent
cavity
solid phase
cavities
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Abandoned
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US13/503,276
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English (en)
Inventor
Alfred Janetzko
Wilhelm Sänger
Cyril E. Geacintov
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DRG Instruments GmbH
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DRG Instruments GmbH
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Assigned to DRG INSTRUMENTS GMBH reassignment DRG INSTRUMENTS GMBH ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: JANETZKO, ALFRED, SANGER, WILHELM, GEACINTOV, CYRIL E.
Publication of US20120252109A1 publication Critical patent/US20120252109A1/en
Abandoned legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object

Definitions

  • the invention relates to a reagent cartridge for an assembly for selectively performing a clinical-chemical test or an ELISA test according to the preamble of claim 1 , use of the reagent cartridge according to claims 11 to 15 , and an assembly according to the preamble of claim 16 .
  • Clinical chemical parameters are typically determined in the liquid phase of a sample. At least one reagent is added to the sample that causes a change in optical density. Then the sample is placed in a measuring cell that is horizontally irradiated by an optical sample beam.
  • the reaction devices must be made of properly resistant material to withstand the often aggressive reagents.
  • Immunodiagnostic parameters however are determined based on the ELISA principle using a substance coupled to a solid phase.
  • the reaction temperature typically is room temperature, i.e. 18° C. to 25° C.
  • Preparation and storage of the antigen- or antibody-coupled solid phase is particularly critical for these tests. It is especially important to optimally bind the antigens or antibodies to the solid phase.
  • EP 1 255 115 A2 therefore proposes an analytical insert for one-time use when performing an ELISA in a fully automated analytical device.
  • the insert is a single-piece molded plastic part comprising multiple cavities.
  • the first cavity accommodates the sample, the other cavities contain reagents for performing the ELISA.
  • the housing also comprises two recesses that are interconnected by a slit. Two interconnected wells that were broken out of a microtiter plate coated with the respective antigen or antibody can be inserted into these recesses.
  • Another disadvantage is that the analytical insert can only be used in devices for performing immunodiagnostic analyses. If it is desirable to determine clinical chemical parameters at the same time, the user has to insert another completely differently composed sample into another device. This can hardly be done in smaller doctor's offices, since it requires the purchase of one expensive device for analyzing immunodiagnostic values and the purchase of a second expensive device for analyzing clinical chemical parameters.
  • the reagent cartridge shall also be suitable for determining immunodiagnostic and clinical chemical parameters in one and the same sample.
  • a reagent cartridge for an assembly for selectively performing a clinical chemical test or an ELISA test comprising a housing having at least one cavity that contains a reaction or diluting component and having a recess, wherein a solid phase to which an antigen or antibody can be coupled can be inserted into said recess of the housing.
  • the reagent cartridge comprises three cavities wherein the first cavity is either used as a diluting cavity, or contains diluent solution, or contains an additional reagent for performing a clinical chemical test, wherein the second cavity is either used as a diluting cavity, or contains conjugate for performing an ELISA or a detection reagent for performing a clinical chemical test, wherein the third cavity is either used as a diluting cavity, or contains substrate for performing an ELISA, or a detection reagent for performing a clinical chemical test or is empty, and wherein either all cavities of the reagent cartridge are used as diluting cavities and/or are empty or wherein the cavities of the reagent cartridge contain a detection reagent for performing a clinical chemical test, conjugate for performing an ELISA, or substrate for performing an ELISA, or are empty.
  • the reagent cartridge is a reagent cartridge for performing a clinical chemical test wherein the first cavity is empty, the second cavity contains a first detection reagent and the third cavity contains a second detection reagent for performing a clinical chemical test. It is also conceivable that the reagent cartridge is a reagent cartridge for performing a clinical chemical test wherein two of the cavities are empty and the third cavity contains a detection reagent for performing a clinical chemical test. It is particularly cost-efficient in manufacturing if the solid phase has neither an antigen nor an antibody bound to it and represents a fourth, empty cavity in the reagent cartridge.
  • the reagent cartridge is a reagent cartridge for performing an immunodiagnostic test wherein the first cavity is empty, the second cavity contains conjugate and the third cavity contains substrate for performing an ELISA, and an antigen or antibody is coupled to the solid phase.
  • the reagent cartridge is a reagent cartridge for diluting a sample wherein at least one of the three cavities contains a diluent solution and at least one of the three cavities is used as diluting cavity. It is favorable with respect to cost and manufacturing effort in this case if neither an antigen nor an antibody is bound to the solid phase and that the solid phase is used as a diluting cavity or is empty.
  • the solid phase is made of a different material than the housing.
  • the solid phase can be made of polystyrene. This plastic primarily enhances binding of the desired antigen or antibody to the solid phase. At the same time however, it is not particularly resistant to many reagents needed for performing clinical chemical tests.
  • the housing consists of another material as mentioned above.
  • the housing is made of polypropylene, a much more resistant material. It is also ensured in this way that the antigen or the antibody will be coupled to the solid phase safely and lastingly while the reagents for performing clinical chemical tests can still be well stored in the reagent cartridge.
  • a reagent is pre-filled in at least one of the cavities while the solid phase is either charged with antibody, or is empty, or is used as diluting cavity.
  • the invention also relates to the use of a reagent cartridge according to the invention for analyzing a clinical chemical parameter in a sample, the use of a reagent cartridge according to the invention for analyzing a immunodiagnostic parameter in a sample, the use of a reagent cartridge according to the invention as a diluting device, and the use of a reagent cartridge according to the invention for providing additional reagents.
  • both clinical chemical parameters and immunodiagnostic components of a sample can be determined in one and the same procedure.
  • the method can be performed in one and the same analytical device. It is therefore no longer necessary to purchase different devices for the analysis of clinical chemical parameters and immunodiagnostic parameters, which means huge cost savings especially for smaller doctor's offices.
  • the special advantage is achieved by determining both the clinical chemical parameters and the immunodiagnostic parameters using a measuring cell and by assigning a reagent cartridge according to the invention to each measuring cell. This is different from the conventional method in which only the clinical chemical parameters are measured using a measuring cell while the immunodiagnostic parameters are measured using a microtiter plate.
  • the analytical device that uses the reagent cartridge of the invention needs to comprise only one photometric unit for analyzing both the clinical chemical and the immunodiagnostic parameters. It is also possible to determine several clinical chemical and/or immunodiagnostic parameters of a sample in one and the same analytical device.
  • a physician may therefore proceed as follows to make a diagnosis: First the physician takes a sample of the body fluid to be analyzed from the patient. Then the physician selects reagent cartridges according to the invention depending on the parameters the physician wishes to determine. The physician inserts the reagent cartridges for each of the parameters into the analytical device and starts the method according to the invention. This method will provide the various desired clinical chemical and immunodiagnostic parameters on which the physician can base the diagnosis.
  • the use of the reagent cartridges according to the invention in such a method provides the advantage that a dedicated reagent cartridge is inserted into a holding fixture of the analytical device for each clinical chemical and/or immunodiagnostic parameter to be determined, and the respective reagent cartridge contains the detection reagents required for the respective test in its cavities. It is preferred that all reagent cartridges used for analyzing clinical chemical parameters contain at least one detection reagent and that reagent cartridges used for analyzing immunodiagnostic parameters contain a first cavity with conjugate, a second cavity with substrate, and a solid phase. It is apparent that the reagent cartridge according to the invention facilitates this is in a particularly advantageous manner.
  • Determination of immunodiagnostic parameters may, for example, include the following steps:
  • conjugate and sample can be picked up in two separate steps and pipetted onto the solid phase.
  • the pipetting device can first pick up the conjugate and dispense it onto the solid phase, then the pipetting device picks up the sample and dispenses it onto the solid phase. It is, of course, also conceivable that the sample is picked up first and dispensed onto the solid phase, then the conjugate.
  • the steps a) through g) correspond to the common steps of performing an ELISA.
  • the immunological parameter to be detected e.g. a specific peptide, antibody, or other protein
  • the conjugate however contains a matching enzyme complex that consists of a binding partner and an enzyme coupled to the binding partner.
  • the binding partner also binds to the parameter to be detected.
  • the entire complex of parameter, binding partner, and enzyme is immobilized on the solid phase in this way. Excess complex and sample are removed or washed out in the next step.
  • the substrate solution added then contains a substrate that is specific to the enzyme immobilized in this way on the solid phase. This substrate is reacted with the enzyme, which causes a color change and thus a defined change in optical density at a specific wavelength.
  • the substrate reaction is ended in that the entire substrate solution that now contains reacted and non-reacted substrate is removed from the solid phase and re-pipetted into a measuring cell for analysis.
  • This makes it possible to determine a multitude of different parameters—primarily immunodiagnostic ones but also clinical chemical ones—always using the same assembly of reagent cartridges and measuring cells.
  • the concentration of the respective parameter can be determined in always the same sequence of steps using the measuring cell and a single photometric unit.
  • the immunodiagnostic parameters using the reagent cartridge according to the invention at a temperature between 27° C. and 39° C., preferably at a temperature of 37° C.
  • This special adjustment of the temperature conditions of the ELISA tests makes it possible that clinical chemical and immunodiagnostic parameters can be determined in any sequence one after the other without having to change the temperature of the analytical device use, which would otherwise involve long cooling and heating phases.
  • Another advantage of raising the temperature is that the temperature is significantly above the regular room temperature. It is therefore irrelevant where the analyzer is placed; the temperature at which the test is performed is always kept constant. The reaction kinetics are always the same as well. This makes test results more repeatable. It is also not required to establish a standard curve for each test as would otherwise be common with ELISA.
  • the components provided in the reagent cartridges according to the invention for performing an immunodiagnostic test are therefore tuned to be of particular advantage when performing the test at a temperature of 37° C.
  • the invention further relates to an assembly for selectively performing a clinical chemical or ELISA test comprising an analytical device, at least one reagent cartridge and at least one measuring cell, wherein the reagent cartridge is a reagent cartridge of the invention according to any one of claims 1 to 11 and wherein a measuring cell is assigned to each reagent cartridge, and the reagent cartridge and the measuring cell 30 assigned to it are in a linear assembly in the analytical device.
  • the analytical device comprises a sample receptacle, a holding fixture for the reagent cartridges and for the measuring cells, and a pipetting device, and that the analytical device comprises an optical unit and a washing unit. It is also favorable if sample receptacle and washing unit are separate from the holding fixture and if the holding fixture is a carousel wherein the reagent cartridges and measuring cells are arranged on the radii of the carousel and wherein the measuring cells are arranged on the outer ends of these radii.
  • FIG. 1 shows a reagent cartridge according to the invention.
  • FIG. 2 shows a cross section through the reagent cartridge of FIG. 1 .
  • FIG. 3 shows a top view of the reagent cartridge of FIG. 1 .
  • FIG. 4 a shows the use of the reagent cartridge from FIG. 2 to perform an ELISA.
  • FIG. 4 b shows the use of the reagent cartridge from FIG. 2 to perform a clinical chemical test.
  • FIG. 4 c shows the use of the reagent cartridge from FIG. 2 as a diluent cartridge.
  • FIG. 4 d shows the use of the reagent cartridge from FIG. 2 for providing additional reagents.
  • FIG. 5 shows the use of a reagent cartridge according to the invention in a fully automated device.
  • FIG. 6 shows a diagrammatic view of an assembly for selectively analyzing clinical chemical and immunodiagnostic parameters in which reagent cartridges of the invention are used.
  • the reagent cartridge 10 shown in FIGS. 1 , 2 , and 3 consists of a housing 11 in which three cavities 12 , 13 , 14 are formed.
  • the housing 11 also comprises a recess 15 into which a solid phase 20 can be inserted.
  • the solid phase 20 has the form of a single well from a microtiter plate and is preferably made of polystyrene while the housing 11 and the cavities 12 , 13 , 14 are made of a resistant material matched to the reagents of the clinical chemical test, such as polypropylene.
  • the housing 11 has a main level 16 with a front end 161 and a rear end 162 .
  • the bottom sides of its front and rear ends 161 , 162 rest on the analytical device and thus secure it in its position (see also FIG. 5 ).
  • the upper openings of the cavities 12 , 13 , 14 are located in the upper level 16 .
  • the recess 15 that is to accommodate the solid phase 20 is formed at a deeper level 18 .
  • the solid phase 20 has been inserted into the recess 15 in FIGS. 1 and 2 . It has a peripheral rim 23 with a projection 22 extending on its end. This projection 22 rests on level 18 which prevents it from slipping through the recess 15 .
  • the upper rim 25 of the solid phase 20 is level with the upper rims of the cavities 12 , 13 , and 14 .
  • the solid phase 20 is reliably and safely stored in the housing 11 of the reagent cartridge 10 . This effectively prevents errors that could result when the user inserts the solid phase 20 incorrectly into the housing 11 . It not only makes the use of the cartridge much easier but results in improved reliability of the measuring results obtained using the reagent cartridge 10 .
  • supporting webs 163 can be formed among the cavities 12 , 13 , and 14 that ensure a stable overall structural design of the reagent cartridge 10 .
  • the supporting webs 163 primarily ensure that the reagent cartridge 10 will always be positioned level when inserted into the analytical device and will not bend, e.g. due to influences from the pipetting device.
  • a respective antigen or antibody suitable for the test can be coupled to the inner wall 21 of the bottom 24 and the side walls 26 of the solid phase 20 . If the reagent cartridge 10 is intended for performing a clinical chemical test, for providing additional reagents, or as a diluent cartridge, the solid phase 20 can be used without an antigen or antibody coupled to it.
  • FIGS. 4 a to 4 d show various options according to the invention for filling the reagent cartridge 10 and providing it in filled condition to a user.
  • the first cavity 14 of the reagent cartridge 10 shown in FIG. 4 a is empty and can be used flexibly for various applications.
  • the second cavity 13 contains an enzyme conjugate K and the third cavity contains a substrate solution S.
  • An antigen or antibody A is coupled to the bottom 24 and the side walls 26 of the solid phase 20 .
  • a pipetting device (not covered by the patent) is used to add enzyme conjugate K and the sample that is stored in a separate container outside the device (not shown) to the solid phase 20 . This can be done by either picking up the enzyme conjugate K and the sample P simultaneously or in two separate pipetting steps. If the immunological component to be detected is contained in the sample, it will bind to the antigen or antibody A that is immobilized on the solid phase 20 . The immunological component also bind the enzyme conjugate K. In this way, both the component to be detected and the enzyme contained in the enzyme conjugate K are also immobilized on the solid phase 20 .
  • the reagent cartridge according to the invention 10 is not charged with enzyme conjugate and substrate solution for performing a clinical chemical test but is filled with a first reagent R 1 and, if required for the specific test, with two or more other reagents R 2 , R 3 in the cavities 12 , 13 , and 14 . It is instead conceivable that the solid phase 20 also accommodates a reagent for performing the test.
  • the manifold uses of the reagent cartridge 10 according to the invention pose a particular advantage.
  • different solid phases 20 can be inserted into the housing 11 . They may consist of one of the materials mentioned or of a third material suitable for the respective application.
  • the reagent cartridge 10 of the invention can be used to accommodate additional reagents.
  • the reagent cartridge 10 shown in FIG. 4 c is simply inserted into the analytical device next to the actual reagent cartridge 10 as shown in FIG. 4 b.
  • another reagent cartridge 10 of the invention may be used as diluent cartridge.
  • the cavities 12 , 13 , 14 and the solid phase 20 may be empty in such a cartridge while the diluent solution is added from a container that is provided separately on the analytical device. It is also conceivable that a diluent solution is already pre-filled at defined volumes in the cavities 12 , 13 , 14 serving as diluent cavities.
  • FIG. 5 It can be seen in FIG. 5 how various reagent cartridges 10 according to the invention are used in a fully automated analytical device 40 . These cartridges are inserted into a carousel-shaped holding fixture 42 of the analytical device 40 such that their cavities 12 , 13 , 14 and the solid phase 20 are always level with a measuring cell 30 assigned to the respective reagent cartridge 10 and used for the photometric analysis of the test results.
  • FIG. 6 shows a general view of the analytical device 40 and the assembly 100 .
  • the analytical device 40 consists of a holding fixture 42 , a sample receptacle 41 , a pipetting device 43 , an optical unit 44 , and a washing unit 45 .
  • the holding fixture 42 comprises both cavities 421 for receiving measuring cells 30 and cavities 422 for receiving reagent cartridges 10 according to the invention. It can be seen that there are one cavity 421 for receiving a measuring cell 30 and one cavity for receiving a reagent cartridge 10 on a common radius R of the holding fixture 42 , wherein the cavity 421 for receiving the measuring cell 30 is located on the outer end of the radius R.
  • the sample receptacle 41 comprises cavities 411 as well. A vial or cup containing the sample to be examined can be inserted into these cavities. It can be seen that the sample receptacle 41 is separate from the holding fixture 42 .
  • the pipetting device 43 consists of a pivotable robot arm 431 that is mounted onto a pivoting mechanism 432 .
  • a pipette that can be washed in the washing unit 45 between pipetting steps, if required, is attached to the bottom side of the robot arm 431 .
  • the robot arm 431 can be pivoted among various positions over the sample receptacle 41 and holding fixture 42 that it can target and pick up either reagents from the inserted reagent cartridges 10 or samples from the sample receptacle 41 and add them selectively to another cavity 12 , 13 , 14 of a reagent cartridge 10 , to the solid phase 20 , or into the measuring cell 30 .
  • measuring cells 30 and reagent cartridges 10 have been inserted into some of the cavities 421 , 422 of the holding fixture 42 .
  • the computer control unit 46 may either be an integral part of the analytical device 40 as in the embodiment shown here or an external computer connected to the analytical device 40 .
  • the robot arm 43 then picks up the reagents and samples according to the method described above and pipettes them back and forth until the measuring cell 30 that is assigned to the reagent cartridge 10 for the respective parameter to be determined contains an optically analyzable solution.
  • the holding fixture 42 is pivoted such that the contents of the measuring cell 30 can be analyzed in the optical unit 44 .
  • the optical unit 44 is a photometric unit that determines the optical density OD of the solution in the measuring cell 30 at a specific wavelength defined for the respective test and passes the result on to the computer for output.
  • Conjugate K and sample may of course be picked up in any sequence as described above and added to the solid phase 20 .
  • the solid phase is washed by either flushing washing solution from the washing station 45 into the solid phase or providing another washing facility (not shown).
  • the invention is not limited to one of the embodiments described above but can be modified in manifold ways.
  • the solid phase 20 comprises a round bottom while the cavities 12 , 13 , 14 have a flat bottom 17 in the embodiments shown above.
  • the cavities 12 , 13 , 14 have a flat bottom 17 in the embodiments shown above.
  • another bottom architecture is conceivable.
  • the reagent cartridge 10 is used as a diluent cartridge, it is conceivable that one or two of the cavities 12 , 13 , 14 are filled with diluent solution while the other cavities 12 , 13 , 14 are empty such that the actual diluting process can be performed in them by adding defined volumes of diluent solution and sample.
  • a reagent cartridge for an assembly for selectively performing a clinical chemical test or an ELISA test comprising a housing 11 having at least one cavity 12 , 13 , 14 that contains a reaction or diluting component and comprises a recess 15 , wherein a solid phase 20 is inserted into the recess 15 of the housing 11 to which an antigen or antibody can be coupled
  • the reagent cartridge 10 comprises three cavities 12 , 13 , 14 ,
  • the reagent cartridge 10 is a reagent cartridge for performing a clinical chemical test, it is useful if the first cavity 14 is empty, the second cavity 13 contains a first detection reagent R 1 , and the third cavity 12 contains a second detection reagent R 2 for performing a clinical chemical test. It is also favorable—if the reagent cartridge 10 is a reagent cartridge for performing a clinical chemical test—if two of the cavities 12 , 13 are empty and the third cavity 14 contains a detection reagent Ra, R 3 for performing a clinical chemical test. It is easy to see the advantage that the solid phase 20 has neither an antigen nor an antibody bound to it and represents a fourth, empty cavity of the reagent cartridge 10 .
  • the reagent cartridge 10 is a reagent cartridge for performing an immunodiagnostic test, it is useful that the first cavity 14 is empty, the second cavity 13 contains enzyme conjugate K, and the third cavity 12 contains substrate S for performing an ELISA, wherein an antigen or antibody A is coupled to the solid phase 20 .
  • the reagent cartridge 10 is a reagent cartridge for diluting a sample, it is useful that at least one of the three cavities 12 , 13 , 14 contains a diluent solution and at least one of the three cavities 12 , 13 , 14 is used as diluting cavity. It is favorable in this case if the solid phase 20 is neither bound to an antigen nor to an antibody and that the solid phase 20 is used as diluting cavity or is empty.
  • the solid phase 20 is made of a different material than the housing 11 .
  • the solid phase 20 is made of polystyrene.
  • a reagent cartridge 10 it is favorable for a reagent cartridge 10 according to the invention that a reagent R 1 , R 2 , R 3 , Ra, K, S is pre-filled in at least one of the cavities 12 , 13 , 14 while the solid phase 20 is either charged with antibody A, is empty or is used as diluting device.
  • Another apparent advantage is the use of a reagent cartridge 10 according to the invention for analyzing a clinical chemical parameter in a sample, the use of a reagent cartridge 10 according to the invention for analyzing a immunodiagnostic parameter in a sample, the use of a reagent cartridge 10 according to the invention as a diluting device, and the use of a reagent cartridge 10 according to the invention for providing additional reagents.
  • the invention further relates to an assembly 100 for selectively performing a clinical chemical or an ELISA test comprising an analytical device 40 , at least one reagent cartridge 10 , and at least one measuring cell 30 , wherein the reagent cartridge is a reagent cartridge of the invention and wherein a measuring cell is assigned to each reagent cartridge, and the reagent cartridge and the measuring cell 30 assigned to it are in a linear assembly in the analytical device.
  • an analytical device 40 comprises a sample receptacle 41 , a holding fixture 42 for the reagent cartridges 10 and for the measuring cells 30 , and a pipetting device 43 , and if the analytical device 40 comprises an optical unit 44 and a washing unit 45 .
  • sample receptacle 41 and the washing unit 45 are separate from the holding fixture 42 and if the holding fixture 42 is a carousel wherein the reagent cartridges 10 and measuring cells 30 are arranged on the radii R of the carousel and wherein the measuring cells 30 are arranged on the outer ends of the radii R.

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US13/503,276 2009-10-30 2010-09-17 Reagent cartridge for an assembly for selectively performing a clincial chemical test or an elisa test, use of said reagent cartridge and assembly Abandoned US20120252109A1 (en)

Applications Claiming Priority (3)

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DE102009051428A DE102009051428A1 (de) 2009-10-30 2009-10-30 Reagenzienpatrone für eine Anordnung zur wahlweisen Durchführung eines klinisch-chemischen Tests oder eines ELISA-Tests
DE102009051428.7 2009-10-30
PCT/EP2010/063732 WO2011051053A1 (de) 2009-10-30 2010-09-17 Reagenzienpatrone für eine anordnung zur wahlweisen durchführung eines klinisch-chemischen tests oder eines elisa-tests, verwendung der reagenzienpatrone und anordnung

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EP (1) EP2493617B1 (de)
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WO2024062057A1 (en) * 2022-09-23 2024-03-28 Diesse Diagnostica Senese S.P.A. Apparatus for performing immunometric tests
WO2024062045A1 (en) * 2022-09-23 2024-03-28 Diesse Diagnostica Senese S.P.A. Apparatus for performing immunometric tests

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DE102010037009A1 (de) * 2010-08-16 2012-02-16 Drg Instruments Gmbh Verfahren zur Analyse einer Probe
AU2013202778A1 (en) * 2013-03-14 2014-10-02 Gen-Probe Incorporated Systems, methods, and apparatuses for performing automated reagent-based assays
WO2016130964A1 (en) 2015-02-13 2016-08-18 Abbott Laboratories Decapping and capping apparatus, systems and methods for use in diagnostic analyzers
FR3058734B1 (fr) * 2016-11-15 2020-10-30 Biomerieux Sa Systeme et procede d’extraction pour extraire des micro-organismes contenus dans un echantillon
CN108792258A (zh) * 2018-05-21 2018-11-13 南京申友生物技术有限公司 一种基因检测用试剂盒

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US20140377785A1 (en) 2014-12-25
JP5797200B2 (ja) 2015-10-21
EP2493617B1 (de) 2019-02-20
CN102686317A (zh) 2012-09-19
US9778255B2 (en) 2017-10-03
JP2013509572A (ja) 2013-03-14
EP2493617A1 (de) 2012-09-05
CN102686317B (zh) 2015-12-02
DE102009051428A1 (de) 2011-05-05
RU2554665C2 (ru) 2015-06-27
WO2011051053A1 (de) 2011-05-05

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