Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
  • A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/14—Vegetable proteins
  • A23J3/16—Vegetable proteins from soybean
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
  • A23L2/385—Concentrates of non-alcoholic beverages
  • A23L2/39—Dry compositions
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
  • A23L2/52—Adding ingredients
  • A23L2/66—Proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
  • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
  • A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
  • A23L2/74—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
  • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
  • A23L2/80—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by adsorption
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
  • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
  • A23L33/17—Amino acids, peptides or proteins
  • A23L33/185—Vegetable proteins
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
  • C11B1/00—Production of fats or fatty oils from raw materials
  • C11B1/10—Production of fats or fatty oils from raw materials by extracting
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
  • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
  • A23V2250/00—Food ingredients
  • A23V2250/15—Inorganic Compounds
  • A23V2250/156—Mineral combination
  • A23V2250/1578—Calcium
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
  • A23V2250/00—Food ingredients
  • A23V2250/54—Proteins
  • A23V2250/548—Vegetable protein
  • A23V2250/5488—Soybean protein

Definitions

• the invention relates to the production of soy protein products.
• soy protein product preferably a soy protein isolate, which is completely soluble at low pH values and is capable of providing transparent and heat stable solutions at such low pH values.
• This soy protein product may be used for protein fortification of, in particular, soft drinks and sports drinks, as well as other acidic aqueous systems, without precipitation of protein.
• the soy protein product is produced by extracting a soy protein source with aqueous calcium chloride solution at natural pH, optionally diluting the resulting aqueous soy protein solution, adjusting the pH of the aqueous soy protein solution to a pH of about 1.5 to about 4.4, preferably about 2.0 to about 4.0, to produce an acidified clear soy protein solution, which may be optionally concentrated and/or diafiltered before drying.
• soy protein product of comparable properties may be formed by a procedure involving extraction of the soy protein source with water followed by calcium chloride addition to the extracted protein solution, before or after concentration. Precipitate formed upon addition of the calcium chloride is removed prior to subsequent processing.
• the soy protein product provided herein is soluble at acid pH values to provide transparent and heat stable aqueous solutions thereof.
• the soy protein product may be used for protein fortification of, in particular, soft drinks and sports drinks, without precipitation of protein.
• the calcium salt solution preferably aqueous calcium chloride
• the calcium salt solution may be added to the aqueous protein solution following separation from the soy protein source and prior to concentrating the solution. Following addition of the calcium chloride, the precipitate formed in the step is removed.
• the resulting aqueous soy protein solution may be further processed by the steps of concentration, dilution, pH adjustment, further concentration and drying, as described above.
• the calcium salt solution preferably aqueous calcium chloride
• the calcium salt solution may be added to the aqueous protein solution following separation from the soy protein source and prior to concentrating the solution. Following addition of the calcium chloride, the precipitate formed in the step is removed.
• the resulting aqueous soy protein solution may be further processed by the steps of partial concentration, dilution, pH adjustment, further concentration and drying.
• the calcium salt solution preferably aqueous calcium chloride may be added to the aqueous soy protein solution following separation from the soy protein source and prior to concentrating the solution. Following addition of the calcium chloride, the precipitate formed in the step is removed.
• the resulting aqueous soy protein solution may be optionally diluted, such as by one volume of water, and then adjusted in pH with acid.
• the acidified solution may then be concentrated and optionally diafiltered to provide a clear, low pH solution ready for drying.
• the calcium salt solution preferably aqueous calcium chloride
• the calcium salt solution may be added to partially concentrated soy protein solution and the resulting precipitate removed from the partially concentrated soy protein solution.
• the clarified solution may then be put back on the membrane system for additional concentration prior to the dilution, pH adjustment, further concentration and drying steps described above.
• the partially concentrated soy protein solution, clarified after calcium chloride treatment may be diluted sufficiently to decrease the conductivity, pH adjusted and then concentrated and diafiltered prior to drying.
• soy protein products of lesser purity may be provided having similar properties to the soy protein isolate.
• Such lesser purity products may have a protein concentration of at least about 60% by weight (N ⁇ 6.25) d.b.
• novel soy protein product of the invention can be blended with powdered drinks for the formation of aqueous soft drinks or sports drinks by dissolving the same in water.
• Such blend may be a powdered beverage.
• the soy protein product provided herein may be provided as an aqueous solution thereof having a high degree of clarity at acid pH values and which is heat stable at these pH values.
• an aqueous solution of the soy product provided herein which is heat stable at low pH.
• the aqueous solution may be a beverage, which may be a clear beverage in which the soy protein product is completely soluble and transparent or an opaque beverage in which the soy protein product does not increase the opacity.
• the soy protein product also has excellent solubility at about pH 7 to about pH 8, providing aqueous solutions with excellent clarity and heat stability.
• An aqueous solution of the soy protein product prepared at about pH 7 to about pH 8 may be a beverage.
• soy protein products produced according to the process herein lack the characteristic beany flavour of soy protein isolates and are suitable, not only for protein fortification of acidic media, but may be used in a wide variety of conventional applications of protein isolates, including but not limited to protein fortification of processed foods and beverages, emulsification of oils, as a body former in baked goods and foaming agent in products which entrap gases.
• the soy protein product may be formed into protein fibers, useful in meat analogs, may be used as an egg white substitute or extender in food products where egg white is used as a binder.
• the soy protein product may be used in nutritional supplements. Other uses of the soy protein products are in pet foods, animal feed and in industrial and cosmetic applications and in personal care products.
• the initial step of the process of providing the soy protein product involves solubilizing soy protein from a soy protein source using water.
• the soy protein source may be soybeans or any soy product or by-product derived from the processing of soybeans including but not limited to soy meal, soy flakes, soy grits and soy flour.
• the soy protein source may be used in the full fat form, partially defatted form or fully defatted form. Where the soy protein source contains an appreciable amount of fat, an oil-removal step generally is required during the process.
• the soy protein recovered from the soy protein source may be the protein naturally occurring in soybean or the proteinaceous material may be a protein modified by genetic manipulation but possessing characteristic hydrophobic and polar properties of the natural protein.
• the solubilization of the protein is effected at a temperature of from about 1° C. to about 100° C., preferably about 15 to about 35° C., preferably accompanied by agitation to decrease the solubilization time, which is usually about 1 to about 60 minutes. It is preferred to effect the solubilization to extract substantially as much protein from the soy protein source as is practicable, so as to provide an overall high product yield.
• the extraction of the protein from the soy protein source is carried out in any manner consistent with effecting a continuous extraction of protein from the soy protein source.
• the soy protein source is continuously mixed with water and the mixture is conveyed through a pipe or conduit having a length and at a flow rate for a residence time sufficient to effect the desired extraction in accordance with the parameters described herein.
• the protein solubilization step is effected rapidly, in a time of up to about 10 minutes, preferably to effect solubilization to extract substantially as much protein from the soy protein source as is practicable.
• the solubilization in the continuous procedure is effected at temperatures between about 1° C. and about 100° C., preferably between about 15° C. and about 35° C.
• the extraction is carried out at the natural pH of the soy protein source/water system, generally about 5 to about 7.
• the pH of the extraction may be adjusted to any desired value within the range of about 1.5 to about 11, preferably about 5 to about 7 by the use of any convenient acid, usually hydrochloric acid, or alkali, usually sodium hydroxide, as required.
• concentration of soy protein source in the water during the solubilization step may vary widely. Typical concentration values are about 5 to about 15% w/v.
• the protein extraction step with the water has the additional effect of solubilizing fats which may be present in the soy protein source, which then results in the fats being present in the aqueous phase.
• the protein solution resulting from the extraction step generally has a protein concentration of about 5 to about 50 g/L, preferably about 10 to about 50 g/L.
• the antioxidant may be any convenient antioxidant, such as sodium sulfite or ascorbic acid.
• the quantity of antioxidant employed may vary from about 0.01 to about 1 wt % of the solution, preferably about 0.05 wt %.
• the antioxidant serves to inhibit the oxidation of any phenolics in the protein solution.
• the aqueous phase resulting from the extraction step then may be separated from the residual soy protein source, in any convenient manner, such as by employing a decanter centrifuge, followed by disc centrifugation and/or filtration to remove residual soy protein source material.
• the separated residual soy protein source may be dried for disposal.
• the separated residual soy protein source be processed to recover some residual protein.
• the separated residual soy protein source may be processed by a conventional isoelectric precipitation procedure or any other convenient procedure to recover such residual protein.
• soy protein source contains significant quantities of fat, as described in U.S. Pat. Nos. 5,844,086 and 6,005,076, assigned to the assignee hereof and the disclosures of which are incorporated herein by reference, then the defatting steps described therein may be effected on the separated aqueous protein solution. Alternatively, defatting of the aqueous protein solution may be achieved by any other convenient procedure.
• the aqueous soy protein solution may be treated with an adsorbent, such as powdered activated carbon or granulated activated carbon, to remove colour and/or odour compounds.
• an adsorbent such as powdered activated carbon or granulated activated carbon
• Such adsorbent treatment may be carried out under any convenient conditions, generally at the ambient temperature of the separated aqueous protein solution.
• powdered activated carbon an amount of about 0.025% to about 5% w/v, preferably about 0.05% to about 2% w/v, is employed.
• the adsorbing agent may be removed from the soy protein solution by any convenient means, such as by filtration.
• the aqueous soy protein solution resulting from the extraction step may be pH adjusted to the range of about 5 to about 7, prior to further processing as discussed below.
• pH adjustment may be effected using any convenient acid, such as hydrochloric acid, or alkali, such as sodium hydroxide, as appropriate.
• the protein solution may be clarified by any convenient procedure such as centrifugation or filtration after the pH adjustment and prior to further processing.
• the aqueous soy protein solution is then concentrated to increase the protein concentration thereof while maintaining the ionic strength thereof substantially constant.
• concentration generally is effected to provide a concentrated protein solution having a protein concentration of about 50 to about 400 g/L, preferably about 100 to about 250 g/L.
• the concentration step may be effected in any convenient manner consistent with batch or continuous operation, such as by employing any convenient selective membrane technique, such as ultrafiltration or diafiltration, using membranes, such as hollow-fibre membranes or spiral-wound membranes, with a suitable molecular weight cut-off, such as about 3,000 to about 1,000,000 Daltons, preferably about 5,000 to about 100,000 Daltons, having regard to differing membrane materials and configurations, and, for continuous operation, dimensioned to permit the desired degree of concentration as the aqueous protein solution passes through the membranes.
• any convenient selective membrane technique such as ultrafiltration or diafiltration
• membranes such as hollow-fibre membranes or spiral-wound membranes
• a suitable molecular weight cut-off such as about 3,000 to about 1,000,000 Daltons, preferably about 5,000 to about 100,000 Daltons, having regard to differing membrane materials and configurations, and, for continuous operation, dimensioned to permit the desired degree of concentration as the aqueous protein solution passes through the membranes.
• the low molecular weight species include low molecular weight materials extracted from the source material, such as carbohydrates, pigments, low molecular weight proteins and anti-nutritional factors, such as trypsin inhibitors, which are themselves low molecular weight proteins.
• the molecular weight cut-off of the membrane is usually chosen to ensure retention of a significant proportion of the protein in the solution, while permitting contaminants to pass through having regard to the different membrane materials and configurations.
• the protein solution may be subjected to a diafiltration step, before or after complete concentration, using water or an aqueous salt solution of the same conductivity and pH as the protein solution.
• a diafiltration may be effected using from about 2 to about 40 volumes of diafiltration solution, preferably about 5 to about 25 volumes of diafiltration solution.
• further quantities of contaminants are removed from the aqueous soy protein solution by passage through the membrane with the permeate.
• the diafiltration operation may be effected until no significant further quantities of contaminants or visible colour are present in the permeate.
• Such diafiltration may be effected using the same membrane as for the concentration step.
• the diafiltration step may be effected using a separate membrane with a different molecular weight cut-off, such as a membrane having a molecular weight cut-off in the range of about 3,000 to about 1,000,000 Daltons, preferably about 5,000 to about 100,000 Daltons, having regard to different membrane materials and configuration.
• a separate membrane with a different molecular weight cut-off such as a membrane having a molecular weight cut-off in the range of about 3,000 to about 1,000,000 Daltons, preferably about 5,000 to about 100,000 Daltons, having regard to different membrane materials and configuration.
• An antioxidant may be present in the diafiltration medium during at least part of the diafiltration step.
• the antioxidant may be any convenient antioxidant, such as sodium sulfite or ascorbic acid.
• the quantity of antioxidant employed in the diafiltration medium depends on the materials employed and may vary from about 0.01 to about 1 wt %, preferably about 0.05 wt %.
• the antioxidant serves to inhibit the oxidation of any phenolics present in the soy protein solution.
• the concentration step and the optional diafiltration step may be effected at any convenient temperature, generally about 2° to about 60° C., preferably about 20° to about 35° C., and for the period of time to effect the desired degree of concentration and diafiltration.
• the temperature and other conditions used to some degree depend upon the membrane equipment used to effect the membrane processing the desired protein concentration of the solution and the efficiency of removal of contaminants to the permeate.
• the concentration and/or diafiltration steps may be operated in a manner favorable for removal of trypsin inhibitors in the permeate along with the other contaminants. Removal of the trypsin inhibitors is promoted by using a membrane of larger pore size, such as about 30,000 to about 1,000,000 Da, operating the membrane at elevated temperatures, such as about 30 to about 60° C. and employing greater volumes of diafiltration medium, such as about 20 to about 40 volumes.
• a reduction in trypsin inhibitor activity may be achieved by exposing soy materials to reducing agents that disrupt or rearrange the disulfide bonds of the inhibitors.
• Suitable reducing agents include sodium sulfite, cysteine and N-acetylcysteine.
• the addition of such reducing agents may be effected at various stages of the overall process.
• the reducing agent may be added with the soy protein source material in the extraction step, may be added to the clarified aqueous soy protein solution following removal of residual soy protein source material, may be added to the concentrated protein solution before or after diafiltration, may be added to the acidified, concentrated protein solution before or after diafiltration, or may be dry blended with the dried soy protein product.
• the addition of the reducing agent may be combined with the membrane processing steps, as described above or a heat treatment step as described below.
• this can be achieved by utilizing a concentration and diafiltration membrane with a smaller pore size, operating the membrane at lower temperatures, employing fewer volumes of diafiltration medium and not employing a reducing agent.
• the concentrated and optionally diafiltered protein solution may be subject to a further defatting operation, if required, as described in U.S. Pat. Nos. 5,844,086 and 6,005,076.
• defatting of the concentrated and optionally diafiltered protein solution may be achieved by any other convenient procedure.
• the concentrated and optionally diafiltered aqueous protein solution may be treated with an adsorbent, such as powdered activated carbon or granulated activated carbon, to remove colour and/or odour compounds.
• an adsorbent such as powdered activated carbon or granulated activated carbon
• Such adsorbent treatment may be carried out under any convenient conditions, generally at the ambient temperature of the concentrated protein solution.
• powdered activated carbon an amount of about 0.025% to about 5% w/v, preferably about 0.05% to about 2% w/v, is employed.
• the adsorbent may be removed from the soy protein solution by any convenient means, such as by filtration.
• the concentrated and optionally diafiltered soy protein solution may be subjected to pasteurization to reduce the microbial load. Such pasteurization may be effected under any desired pasteurization conditions. Generally, the concentrated and optionally diafiltered soy protein solution is heated to a temperature of about 55° to about 70° C., preferably about 60° to about 65° C., for about 30 seconds to about 60 minutes, preferably about 10 minutes to about 15 minutes. The pasteurized concentrated soy protein solution then may be cooled for further processing as described below, preferably to a temperature of about 20° to about 35° C.
• a calcium salt usually calcium chloride solution
• a calcium salt is added to the resulting solution. This addition causes the formation of a precipitate containing primarily phytate.
• Sufficient calcium chloride is added to provide a solution having a conductivity generally of about 5 to about 30 mS, preferably of about 15 to about 25 mS.
• calcium salt is usually effected using calcium chloride solution
• solutions of other calcium salts may be used.
• the calcium salt may be added in dry form.
• other alkaline earth metal salts may be used.
• the addition of the calcium salt may be effected at a temperature of about 2° C. to about 70° C., preferably about 20° C. to about 35° C.
• the precipitated material is removed from the protein solution by any convenient means, such as by centrifugation or filtration.
• the concentrated protein solution from the phytate precipitation is then diluted by mixing the retentate with water having a volume required to achieve the degree of dilution desired.
• the concentrated protein solution generally is diluted about 2 to about 20 fold, preferably about 10 to about 15 fold.
• the water with which the concentrated protein solution is mixed has a temperature of about 2° to about 90° C., preferably about 10° to about 50° C., more preferably about 20° to about 30° C.
• Dilution of the concentrated protein solution results in the formation of a protein precipitate. Acidification of the diluted solution re-solubilizes the protein and results in a transparent solution further processed as detailed below. Alternatively, the precipitate may be collected and dried by any convenient means.
• the diluted retentate then is adjusted in pH to about 1.5 to about 4.4, preferably about 2.0 to about 4.0, by the addition of any suitable acid, such as hydrochloric acid or phosphoric acid, to result in a clear aqueous soy protein solution.
• the diluted and acidified protein solution may optionally be polished by any convenient means such as filtration.
• the acidified clear soy protein solution may be subjected to a heat treatment to inactivate heat labile anti-nutritional factors, such as trypsin inhibitors, present in such solution as a result of extraction from the soy protein source material during the extraction step.
• a heating step also provides the additional benefit of reducing the microbial load.
• the protein solution is heated to a temperature of about 70° C. to about 160° C., preferably about 80° C. to about 120° C., more preferably about 85° C. to about 95° C., for about 10 seconds to about 60 minutes, preferably about 30 seconds to about 5 minutes.
• the heat treated acidified soy protein solution then may be cooled for further processing as described below, preferably to a temperature of about 2° C. to about 60° C., preferably about 20° C. to about 35° C.
• the acidified clear soy protein solution is concentrated to increase the protein concentration thereof while maintaining the ionic strength thereof substantially constant.
• concentration generally is effected to provide a concentrated protein solution having a protein concentration of about 50 to about 300 g/L, preferably about 100 to about 200 g/L.
• the concentration step may be effected in any convenient manner consistent with batch or continuous operation, such as by employing any convenient selective membrane technique, such as ultrafiltration or diafiltration, using membranes, such as hollow-fibre membranes or spiral-wound membranes, with a suitable molecular weight cut-off, such as about 3,000 to about 1,000,000 Daltons, preferably about 5,000 to about 100,000 Daltons, having regard to differing membrane materials and configurations, and, for continuous operation, dimensioned to permit the desired degree of concentration as the aqueous protein solution passes through the membranes.
• any convenient selective membrane technique such as ultrafiltration or diafiltration
• membranes such as hollow-fibre membranes or spiral-wound membranes
• a suitable molecular weight cut-off such as about 3,000 to about 1,000,000 Daltons, preferably about 5,000 to about 100,000 Daltons, having regard to differing membrane materials and configurations, and, for continuous operation, dimensioned to permit the desired degree of concentration as the aqueous protein solution passes through the membranes.
• the low molecular weight species include not only the ionic species of the food grade salt but also low molecular weight materials extracted from the source material, such as carbohydrates, pigments, low molecular weight proteins and anti-nutritional factors.
• the molecular weight cut-off of the membrane is usually chosen to ensure retention of a significant proportion of the protein in the solution, while permitting contaminants to pass through having regard to the different membrane materials and configurations.
• the protein solution may be subjected to a diafiltration step, before or after complete concentration, using water or a dilute saline solution.
• the diafiltration solution may be at its natural pH or at a pH equal to the protein solution being diafiltered or at any pH value in between.
• Such diafiltration may be effected using from about 2 to about 40 volumes of diafiltration solution, preferably about 5 to about 25 volumes of diafiltration solution. In the diafiltration operation, further quantities of contaminants are removed from the clear aqueous soy protein solution by passage through the membrane with the permeate.
• the diafiltration operation may be effected until no significant further quantities of contaminants or visible colour are present in the permeate or until the retentate has been sufficiently purified so as, when dried, to provide a dry isolate with a protein content of at least about 90 wt % on a dry basis.
• Such diafiltration may be effected using the same membrane as for the concentration step.
• the diafiltration step may be effected using a separate membrane with a different molecular weight cut-off, such as a membrane having a molecular weight cut-off in the range of about 3,000 to about 1,000,000 Daltons, preferably about 5,000 to about 100,000 Daltons, having regard to different membrane materials and configuration.
• An antioxidant may be present in the diafiltration medium during at least part of the diafiltration step.
• the antioxidant may be any convenient antioxidant, such as sodium sulfite or ascorbic acid.
• the quantity of antioxidant employed in the diafiltration medium depends on the materials employed and may vary from about 0.01 to about 1 wt %, preferably about 0.05 wt %.
• the antioxidant serves to inhibit the oxidation of any phenolics present in the soy protein solution.
• the concentration step and the optional diafiltration step may be effected at any convenient temperature, generally about 2° C. to about 60° C., preferably about 20° C. to about 35° C., and for the period of time to effect the desired degree of concentration and diafiltration.
• the temperature and other conditions used to some degree depend upon the membrane equipment used to effect the membrane processing the desired protein concentration of the solution and the efficiency of the removal of the contaminants to the permeate.
• trypsin inhibitors in soy There are two main trypsin inhibitors in soy, namely the Kunitz inhibitor, which is a heat-labile molecule with a molecular weight of approximately 21,000 Daltons, and the Bowman-Birk inhibitor, a more heat-stable molecule with a molecular weight of about 8,000 Daltons.
• Kunitz inhibitor which is a heat-labile molecule with a molecular weight of approximately 21,000 Daltons
• Bowman-Birk inhibitor a more heat-stable molecule with a molecular weight of about 8,000 Daltons.
• the level of trypsin inhibitor activity in the final soy protein product can be controlled by manipulation of various process variables.
• heat treatment of the acidified clear soy protein solution may be used to inactivate heat-labile trypsin inhibitors.
• the partially concentrated or fully concentrated acidified clear soy protein solution may also be heat treated to inactivate heat labile trypsin inhibitors.
• Acidifying and membrane processing the diluted protein solution at a lower pH may reduce the trypsin inhibitor activity relative to processing the solution at higher pH (3.0 to 4.4).
• the protein solution is concentrated and diafiltered at the low end of the pH range, it may be desired to raise the pH of the retentate prior to drying.
• the pH of the concentrated and diafiltered protein solution may be raised to the desired value, for example pH 3, by the addition of any convenient food grade alkali such as sodium hydroxide.
• the concentration and/or diafiltration steps may be operated in a manner favorable for removal of trypsin inhibitors in the permeate along with the other contaminants. Removal of the trypsin inhibitors is promoted by using a membrane of larger pore size, such as about 30,000 to about 1,000,000 Da, operating the membrane at elevated temperatures, such as about 30 to about 60° C. and employing greater volumes of diafiltration medium, such as about 20 to about 40 volumes.
• a reduction in trypsin inhibitor activity may be achieved by exposing soy materials to reducing agents that disrupt or rearrange the disulfide bonds of the inhibitors.
• Suitable reducing agents include sodium sulfite, cysteine and N-acetylcysteine.
• this can be achieved by utilizing a concentration and diafiltration membrane with a smaller pore size, operating the membrane at lower temperatures, employing fewer volumes of diafiltration medium and not employing a reducing agent.
• the concentrated and optionally diafiltered aqueous acidified protein solution may be treated with an adsorbent, such as powdered activated carbon or granulated activated carbon, to remove colour and/or odour compounds.
• an adsorbent such as powdered activated carbon or granulated activated carbon
• Such adsorbent treatment may be carried out under any convenient conditions, generally at the ambient temperature of the concentrated protein solution.
• powdered activated carbon an amount of about 0.025% to about 5% w/v, preferably about 0.05% to about 2% w/v, is employed.
• the adsorbent may be removed from the soy protein solution by any convenient means, such as by filtration.
• the concentrated and optionally diafiltered acidified clear aqueous soy protein solution may be dried by any convenient technique, such as spray drying or freeze drying.
• the pasteurization step described above may be effected on the soy protein solution prior to drying.
• the dry soy protein product has a protein content, in excess of about 60 wt % protein.
• the dry soy protein product is an isolate containing at least about 90 wt % protein, more preferably at least about 100 wt % (N ⁇ 6.25).
• the soy protein product is low in phytic acid content, generally less than about 1.5% by weight.
• the soy protein product produced herein is soluble in an acidic aqueous environment, making the product ideal for incorporation into beverages, both carbonated and uncarbonated, to provide protein fortification thereto.
• beverages have a wide range of acidic pH values, ranging from about 2.5 to about 5.
• the soy protein products provided herein may be added to such beverages in any convenient quantity to provide protein fortification to such beverages, for example, to supply at least about 5 g of soy protein per serving.
• the added soy protein product dissolves in the beverage and does not impair the clarity of the beverage, even after thermal processing.
• the soy protein product may be blended with dried beverage prior to reconstitution of the beverage by dissolution in water. In some cases, modification of the normal formulation may be necessary where components present in the beverage may adversely affect the ability of the composition to remain dissolved in the beverage.
• This Example illustrates the preparation of a dried soy protein isolate in accordance with one embodiment of the invention.
• the 293 L of filtered protein solution was reduced in volume to 28 kg by concentration on a PVDF membrane having a molecular weight cutoff of 5,000 daltons.
• the concentrated protein solution was then diafiltered with 140 L of reverse osmosis purified water.
• the resulting diafiltered, concentrated protein solution had a protein content of 7.35% by weight and represented a yield of 83.5 wt % of the initial filtered protein solution.
• the diafiltered, concentrated protein solution was then dried to yield a product found to have a protein content of 101.96% (N ⁇ 6.25) d.b.
• the product was termed S005-A14-09A S800.
• a 3.2% w/v protein solution of S005-A14-09A S800 in water was prepared and the colour and clarity assessed using a HunterLab ColorQuest XE instrument operated in transmission mode. The pH was also measured with a pH meter.
• This Example contains an evaluation of the heat stability in water of the soy protein isolate produced by the method of Example 1 (S800).
• a 2% w/v protein solution of S005-A14-09A S800 in water was produced and the pH adjusted to 3.
• the clarity of this solution was assessed by haze measurement with the HunterLab.
• the solution was then heated to 95° C., held at this temperature for 30 seconds and then immediately cooled to room temperature in an ice bath. The clarity of the heat treated solution was then measured again.
• This Example contains an evaluation of the solubility in water of the soy protein isolate produced by the method of Example 1 (S800). Solubility was tested based on protein solubility (termed protein method, a modified version of the procedure of Mon et al., J. Food Sci. 50:1715-1718) and total product solubility (termed pellet method).
• the samples were made up to 50 ml total volume with RO water, yielding a 1% w/v protein dispersion.
• the protein content of the dispersions was measured using a Leco FP528 Nitrogen Determinator. Aliquots (20 ml) of the dispersions were then transferred to pre-weighed centrifuge tubes that had been dried overnight in a 100° C. oven then cooled in a desiccator and the tubes capped. The samples were centrifuged at 7800 g for 10 minutes, which sedimented insoluble material and yielded a clear supernatant.
• the protein content of the supernatant was measured by Leco analysis and then the supernatant and the tube lids were discarded and the pellet material dried overnight in an oven set at 100° C. The next morning the tubes were transferred to a desiccator and allowed to cool. The weight of dry pellet material was recorded. The dry weight of the initial protein powder was calculated by multiplying the weight of powder used by a factor of ((100 ⁇ moisture content of the powder (%))/100). Solubility of the product was then calculated two different ways:
• Solubility (protein method) (%) (% protein in supernatant/% protein in initial dispersion) ⁇ 100 1)
• This Example contains an evaluation of the clarity in water of the soy protein isolate produced by the method of Example 1 (S800).
• the clarity of the 1% w/v protein solutions prepared as described in Example 3 was assessed by measuring the absorbance at 600 nm, with a lower absorbance score indicating greater clarity. Analysis of the samples on a HunterLab ColorQuest XE instrument in transmission mode also provided a percentage haze reading, another measure of clarity.
• This Example contains an evaluation of the solubility in a soft drink (Sprite) and sports drink (Orange Gatorade) of the soy protein isolate produced by the method of Example 1 (S800).
• the solubility was determined with the protein added to the beverages with no pH correction and again with the pH of the protein fortified beverages adjusted to the level of the original beverages.
• Solubility (%) (% protein in supernatantl% protein in initial dispersion) ⁇ 100
• Solubility (%) (% protein in supernatant/% protein in initial dispersion) ⁇ 100
• This Example contains an evaluation of the clarity in a soft drink and sports drink of the soy protein isolate produced by the method of Example 1 (S800).
• Example 5 The clarity of the 2% w/v protein dispersions prepared in soft drink (Sprite) and sports drink (Orange Gatorade) in Example 5 were assessed using the methods described in Example 4. For the absorbance measurements at 600 nm, the spectrophotometer was blanked with the appropriate beverage before the measurement was performed.
• This Example contains an evaluation of the clarity and heat stability in water at pH 7 to 8 of the soy protein isolate produced by the method of Example 1 (S800).
• a 2% w/v protein solution of S005-A14-09A S800 in water was produced and the pH adjusted to 7, 7.5 or 8 with diluted sodium hydroxide.
• the clarity of these solutions was assessed by haze measurement with the HunterLab. The solution was then heated to 95° C. then allowed to cool to room temperature. The clarity of the heat treated solution was then measured again.
• the present invention provides a novel procedure for forming a soy protein product that is soluble in acidic media and forms heat stable and transparent solutions therein. Modifications are possible within the scope of this invention.

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