US20090028961A1 - Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms - Google Patents

Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms Download PDF

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Publication number
US20090028961A1
US20090028961A1 US11/913,094 US91309406A US2009028961A1 US 20090028961 A1 US20090028961 A1 US 20090028961A1 US 91309406 A US91309406 A US 91309406A US 2009028961 A1 US2009028961 A1 US 2009028961A1
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mixture
buffer system
decontamination solution
microorganisms
proteins
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Thomas Lisowsky
Karlheinz Esser
Richard Lisowsky
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Multibind Biotec GmbH
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Multibind Biotec GmbH
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Publication of US20090028961A1 publication Critical patent/US20090028961A1/en
Priority to US13/867,089 priority Critical patent/US20130243885A1/en
Priority to US14/936,713 priority patent/US20160081348A1/en
Priority to US15/722,153 priority patent/US10980239B2/en
Abandoned legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
    • A01N31/14—Ethers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
    • A01N43/76—1,3-Oxazoles; Hydrogenated 1,3-oxazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
    • A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02—Local antiseptics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • the invention concerns a decontamination solution for the treatment of surfaces that are contaminated by unwanted proteins, nucleic acid molecules or microorganisms.
  • the invention further concerns the use of said decontamination solution and a suitable buffer system.
  • PCR polymerase chain reaction
  • An object of the here described invention is therefore to overcome the disadvantages of the prior art and to develop new methods and solutions that do not use aggressive chemicals or highly oxidizing agents and that in addition completely decontaminate the treated substrates.
  • this object is achieved by a decontamination solution comprising a synergistic mixture of
  • the mixture has a pH value ranging between pH 3 and 7, preferably between pH 4 and 6.
  • the solution according to the invention is stable over a long period of time and allows for very efficient degradation of nucleic acids.
  • the skin-compatibility of the solution according to the invention is optimal in the range between pH 4 and 6.
  • the vitamins or their respective salts or acidic derivates contained in the solution according to the invention are one or several compounds and/or their related salts selected from the group of the water-soluble vitamins with the properties of antioxidants, like preferably vitamin C, riboflavine and niacin.
  • they are used in concentrations of 1 mM to 1000 mM in relation to the total volume of the solution, in particular in a concentration of 10 mM to 100 mM.
  • the surface-active substances contained in the solution according to the invention may be, for example, anionic, non-ionic, amphoteric or cationic inert tensides or suitable mixtures thereof or thereunder.
  • alkylethersulfate, alkyl- and/or arylsulfonate, alkylsulfate, amphotensides, betaines, alkylamidoalkylamines, alkyl substituted amino acids, alkyl substituted imino acids, acylated amino acids, and amphotenside combinations can be used.
  • inert tensides are suitable. Inert means, that they do not influence the synergistic solution and the experimental outcome.
  • anionic and non-ionic tensides are preferred. They are preferably used in concentrations of 0.1% to 10% (weight), in relation to the total volume of the solution, in particular in concentrations of 0.2% to 0.5% (weight).
  • the decontamination solutions according to the invention may comprise additional common inert adjuvants and additives like for example suitable buffer substances for adjusting a specific pH value, like, for example, Tris (Tris(hydroxymethyl)-aminomethan), MES (2(Morpholino)ethansulfonic acid), HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethansulfonic acid, and/or MOPS (3-(N-Morpholino)propansulfonic acid).
  • suitable buffer substances for adjusting a specific pH value like, for example, Tris (Tris(hydroxymethyl)-aminomethan), MES (2(Morpholino)ethansulfonic acid), HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethansulfonic acid, and/or MOPS (3-(N-Morpholino)propansulfonic acid).
  • the efficient action of the new three component system is even more surprising as it is proven that the different isolated substances alone do not exhibit a special degradation effect and also the mixtures of the components outside the range of the invention are not effective or do only show an unsatisfactory effect. Only the combination of vitamins with metal ions and detergents, preferably in an appropriate mixture, results in a synergistic effect and in a rapid and massive degradation of the biomolecules. In particular, by keeping the correct preferred concentrations, an efficient activity of the decontamination solution according to the invention is ensured.
  • decontamination is achieved by spraying or rubbing the inventive solutions onto contaminated surfaces or by immersion.
  • a residence time of 0.5 to 2 minutes at room temperature or slightly higher temperatures is normally sufficient for complete denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acids and microorganisms from surfaces.
  • the applied methods are however variable and can be adjusted to the different tasks.
  • Another purpose of this invention is the use of the inventive decontamination solutions for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms from surfaces.
  • the new and advantageous buffer system with carbonate and derivatives of succinic acid is especially suitable for the decontamination solution of the present invention.
  • the different mixtures with pH values between pH 2 and 8.5 each provide clear solutions of a light yellow to light brown color that are stable over longer periods of time and that also show in particular in the pH range of pH 4.5 to pH 6 a very efficient degradation of DNA molecules as demonstrated in comparison to the strong mineralic 0.5 M phosphoric acid of pH 1.5, as shown in FIG. 5 .
  • the solution comprising the buffer system may be advantageously adjusted to a pH value ranging from pH 2 to 8.5, in particular from 3 to 7, preferably from 4 to 6. Thereby it is ensured that the further components dissolved in the solution are not impaired and additionally a skin-compatible solution can be prepared.
  • the invention also concerns a method for adjusting the pH value of a solution which comprises a mixture of
  • the mixture can be adjusted by the buffer system according to the invention to a pH value ranging from 2 to 8.5, in particular from 3 to 7, preferably from 4 to 6.
  • FIG. 1 to 5 show the efficient degradation of DNA molecules by the three component system in comparison with known other solutions of prior art.
  • FIG. 6 demonstrates the blockage of PCR amplification of DNA molecules after the treatment with the new three component system.
  • FIG. 7 shows the standard test with RNaseA for enzyme inactivation with the new three component system.
  • FIG. 8 shows the efficient degradation of genomic DNA and extra chromosomal genetic material inside microorganisms by the new three component system.
  • Table 1 shows a test for the anti-microbial action of the new three component system.
  • Table 2 shows the preferred basic composition and the preferred mixtures for the three component system containing detergents, vitamins and metal ions.
  • FIGS. 1 to 5 show the efficient degradation of DNA molecules by the new three component system in comparison with known other solutions.
  • Identical aliquots of DNA plasmids (YEp351) were treated for 2 minutes with the listed solutions. Afterwards the DNA samples were denatured and the single-stranded DNA molecules were separated by gel electrophoresis on an agarose gel (1%). After staining with ethidium bromide the listed pictures were produced.
  • the control shows intact plasmid DNA after treatment with sterile water. Introduction of nicks into the DNA strand results in a reduction of the size and molecular weight of the respective DNA molecules. This effect can be identified in the gel by comparison with the control and the molecular weight marker.
  • FIG. 1 shows the comparison of nucleic acid degradation by vitamins and metal ions alone and by the three component system containing vitamins, metal ions and detergents.
  • M Marker DNA 1 kb ladder
  • C Control: DNA+5 ⁇ l sterile H 2 O
  • 1 5 mM FeCl 3
  • 2 1 mM FAD
  • 3 1 mM FAD+1 mM FeCl 3
  • 4 100 mM NAD
  • 5 100 mM NAD+5 mM FeCl 3
  • 6 100 mM thiamin
  • 7 100 mM thiamin+5 mM FeCl 3
  • 8 100 mM vitamin C
  • 9 100 mM vitamin C+5 mM FeCl 3
  • 10 100 mM Na-ascorbate
  • 11 100 mM Na-ascorbate+5 mM FeCl 3
  • 12 100 mM ascorbic acid+5 mM FeCl 3 ). All samples contained 0.
  • FIG. 2 shows the test for nucleic acid degradation by mixtures containing vitamin C, metal ions and detergents.
  • L 1 Kb Ladder; M: Marker DNA Lambda EcoRI/HindIII; 1: 10 mM vitamin C; 2: 100 mM vitamin C; 3: 10 mM FeCl 3 ; 4: 100 mM vitamin C+10 mM FeCl 3 ; 5: 10 mM ZnCl 2 ; 6: 100 mM vitamin C+10 mM ZnCl 2 ; 7: DNA-OFFTM; C: Control: 5 ⁇ l sterile H 2 O). All samples contained 0.2% Triton X-100 and 0.2% Tween 20.
  • FIG. 3 shows the comparison of nucleic acid degradation by mixtures containing ascorbic acid or Na-ascorbate.
  • M Marker DNA Lambda EcoRI/HindIII
  • C Control: 5 ⁇ l sterile H 2 O
  • 1 DNA-OFFTM
  • 2 100 mM HAc+10 mM FeCl 3 30 0.2% Triton X-100
  • 3 100 mM ascorbic acid+0.2% TritonX-100
  • 4 100 mM ascorbic acid+10 mM FeSO 4 +0.2% TritonX-100
  • 5 100 mM ascorbic acid+ZnCl 2 +0.2% TritonX-100
  • 6 100 mM Na-ascorbate+0.2% TritonX-100
  • 7 100 mM Na-ascorbate+10 mM FeCl 3 +0.2% Triton X-100
  • 8 100 mM Na-ascorbate+ZnCl 2 +0.2% Triton X
  • FIG. 4 shows the comparison of nucleic acid degradation by mixtures containing mineralic acids, ascorbic acid or Na-ascorbate (pH 6 to 8.5).
  • M Marker DNA Lambda EcoRI/HindIII C: Control: DNA+5 ⁇ l sterile H 2 O; 1: RNase-OFFTM (sample 1); 2: RNase-OFFTM (sample 2); 3: DNA-OFFTM (sample 1); 4: DNA-OFFTM (sample 2); 5: 0.5 M H 3 PO 4 ; 6: 0.5 M HNO 3 ; 7: 100 mM ascorbic acid+10 mM FeCl 3 ; 8: 10 mM ascorbic acid+10 mM FeCl 3 ; 9: 100 mM Na-ascorbate+10 mM FeCl 3 ; 10: mixture like in 9 only with pH 6; 11: mixture like in 9 only with pH 7.1; 12: mixture like in 9 only with pH 8; 13: mixture like in 9 only with pH
  • FIG. 5 shows an example for the new buffer system containing Na-carbonate and malic (hydroxy-succinic) acid in comparison with mineralic acid.
  • the basic mixture contains 50 mM ascorbic acid, 5 mM FeCl 3 and 0.2% Triton X-100 and 0.2% Tween 20.
  • M Marker DNA 1 kb Ladder
  • C Control: DNA+5 ⁇ l sterile H 2 O; 1: mixture with pH 10; 2: mixture with pH 9; 3: mixture with pH 7; 4: mixture with pH 6; 5: mixture with pH 4; 6: 0.5 M H 3 PO 4 (pH 1.5) with 0.2% Triton X-100 and 0.2% Tween 20.
  • FIG. 6 shows the blockage of PCR amplification for DNA molecules after treatment with the new three component system (mixture A: 100 mM vitamin C+10 mM FeCl 3 +0.2% Triton X-100 and 0.2% Tween 20).
  • Mixture A 100 mM vitamin C+10 mM FeCl 3 +0.2% Triton X-100 and 0.2% Tween 20.
  • Different amounts (0.1 to 1 ng) of a DNA sample were dried in PCR tubes.
  • PCR tubes containing the dried DNA were treated for 20 seconds with the listed solutions. Subsequently the tubes were washed two-times with 100 ⁇ l of sterile, distilled water. Finally the tubes were filled with a 50 ⁇ l PCR reaction mixture and the PCR reaction was performed.
  • the PCR reaction mixture contained pair of primers for the amplification of the control DNA (scIMP2 gene of yeast) and the test DNA (scPCP1 gene of yeast).
  • the control DNA (1 ng) indicates a successful PCR reaction.
  • a band of the test DNA demonstrates that intact DNA molecules for this gene are still present. In case of complete removal or blockage of the test DNA there shall not be any amplified DNA band in the gel.
  • DNA was stained with ethidium bromide after gel electrophoresis in a 1% agarose gel and the gel was photographed.
  • a positive control the conventional DNA-OFFTM was used.
  • Sterile water (H 2 O) served as a negative control.
  • FIG. 7 shows the test of prior art for RNaseA enzyme inactivation in comparison with the new three component system.
  • Identical aliquots of 10 ⁇ g RNaseA were dried in Eppendorf tubes. Afterwards each tube was treated with 1 ml of the listed solutions, vortexed for 20 seconds and finally incubated for 5 minutes at room temperature. Subsequently each tube was washed two-times with 1 ml of sterile water. Afterwards 5 ⁇ g of total RNA from E. coli were added into the tubes and incubated for 30 minutes at 37° C. Subsequently total RNA samples were mixed with formamide/bromphenol blue buffer and denatured at 95° C. for 5 minutes.
  • RNA sample was loaded onto an agarose gel (1.2%) and separated by gel electrophoresis. After staining with ethidium bromide the documented picture was taken. The untreated controls represent intact total RNA. In the presence of active RNaseA these RNA molecules are degraded. In case of successful, complete inactivation of RNaseA the total RNA molecules will also remain intact.
  • C Control of total RNA
  • 1 RNase-OFF (A); 2: RNase-OFF (B); 3: ascorbic acid mixture with 100 mM vitamin C+10 mM FeCl 3 +0.5% SDS; 4: empty lane; 5: H 2 O; 6: 10 ⁇ g RNase A; 7: empty lane; C: Control of total RNA
  • FIG. 8 shows the efficient degradation of genomic DNA and extra-chromosomal genetic material inside microorganisms by the new three component system.
  • a recombinant Escherichia coli strain with an extra-chromosomal plasmid (YEp351) was grown over night in LB, amp medium. Aliquots of 5 ⁇ l from the E. coli suspension were treated with 5 ⁇ l lysozyme solution (1 mg/ml) for 5 minutes and subsequently incubated with 5 ⁇ l of the listed solutions (1-5) for additional 5 minutes. After addition of bromphenol blue the samples were loaded into the gel slots and separated by electrophoresis of the DNA molecules. Only for sample number 5 with the three component system a massive degradation of the DNA molecules is identifiable.
  • Table 1 shows the test for antimicrobial action of the new three component system.
  • Freshly grown cultures of the listed microorganisms were adjusted to a cell number of 10 in a 50 ⁇ l volume and mixed in a ratio of 1:1 with 50 ⁇ l of water, 70% ethanol or the three component system (100 mM ascorbic acid, 10 mM FeCl 3 and 0.5% SDS). After an incubation time of 2 minutes the 100 ⁇ l samples containing the microorganisms were plated onto the respective growth media. After an incubation period of 1-3 days at 28° C. ( Saccharomyces cerevisiae and Candida parapsilosis ) or 37° C. ( Escherichia coli and Bacillus subtilis ) the number of grown colonies was determined. In test samples with sterile water all microorganisms survived. Test samples with 70% ethanol or the three component system did not show any living cell colony, indicating that under these conditions all microorganisms were killed.
  • Table 2 summarizes the preferred composition of the solution with the three component system comprising surface-active substances, vitamins and metal ions for removal of DNA molecules from surfaces and equipments.
  • composition of solutions containing the three component system comprising surface-active substances, vitamins and metal ions for the removal of DNA molecules from surfaces and equipment.
  • composition of the solutions pH range: 2.0 to 8.5 vitamins: 1 mM to 100 mM metal ions: 1 mM to 50 mM detergents: 0.1% to 5%

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US11/913,094 2005-04-30 2006-05-02 Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms Abandoned US20090028961A1 (en)

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US13/867,089 US20130243885A1 (en) 2005-04-30 2013-04-21 Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms
US14/936,713 US20160081348A1 (en) 2005-04-30 2015-11-10 Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms
US15/722,153 US10980239B2 (en) 2005-04-30 2017-10-02 Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms

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DE102005020327A DE102005020327A1 (de) 2005-04-30 2005-04-30 Dekontaminationslösungen und deren Verwendung zur Denaturierung, Modifikation, Degradation, Solubilisierung und Entfernung von Proteinen, Nukleinsäuremolekülen und Mikroorganismen von Oberflächen
DE102005020327.2 2005-04-30
PCT/DE2006/000758 WO2006116983A2 (fr) 2005-04-30 2006-05-02 Solutions de decontamination et leur utilisation pour denaturer, modifier, degrader, rendre solubles et eliminer des proteines, molecules d'acide nucleique et micro-organismes

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US13/867,089 Abandoned US20130243885A1 (en) 2005-04-30 2013-04-21 Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms
US14/936,713 Abandoned US20160081348A1 (en) 2005-04-30 2015-11-10 Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms
US15/722,153 Active US10980239B2 (en) 2005-04-30 2017-10-02 Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms

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US14/936,713 Abandoned US20160081348A1 (en) 2005-04-30 2015-11-10 Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms
US15/722,153 Active US10980239B2 (en) 2005-04-30 2017-10-02 Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms

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EP (1) EP1881763B1 (fr)
JP (1) JP4918084B2 (fr)
AT (1) ATE504208T1 (fr)
CA (1) CA2604223C (fr)
DE (2) DE102005020327A1 (fr)
ES (1) ES2364186T3 (fr)
NO (1) NO338200B1 (fr)
WO (1) WO2006116983A2 (fr)

Cited By (6)

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Publication number Priority date Publication date Assignee Title
US20100119566A1 (en) * 2007-06-28 2010-05-13 Bode Chemie Gmbh Use of a synergistic compositions as a therapeutic agent or disinfectant
US8470755B1 (en) 2012-03-23 2013-06-25 The Procter & Gamble Company Liquid cleaning and disinfecting compositions comprising a zinc inorganic salt
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US9795133B2 (en) 2011-01-26 2017-10-24 Multibind Biotec Gmbh Synergistic disinfecting compositions with essential oils
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US8557265B2 (en) * 2007-06-28 2013-10-15 Bode Chemie Gmbh Use of a synergistic composition as a therapeutic agent of disinfectant
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US9795133B2 (en) 2011-01-26 2017-10-24 Multibind Biotec Gmbh Synergistic disinfecting compositions with essential oils
US8470755B1 (en) 2012-03-23 2013-06-25 The Procter & Gamble Company Liquid cleaning and disinfecting compositions comprising a zinc inorganic salt
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WO2006116983A3 (fr) 2007-06-21
CA2604223A1 (fr) 2006-11-09
ATE504208T1 (de) 2011-04-15
US20160081348A1 (en) 2016-03-24
EP1881763B1 (fr) 2011-04-06
DE502006009255D1 (de) 2011-05-19
JP2008540332A (ja) 2008-11-20
ES2364186T3 (es) 2011-08-26
DE102005020327A1 (de) 2006-11-09
NO20076045L (no) 2007-11-23
EP1881763A2 (fr) 2008-01-30
CA2604223C (fr) 2013-04-09
US10980239B2 (en) 2021-04-20
NO338200B1 (no) 2016-08-01

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