US10695347B2 - Pyrimidine derivative and use thereof - Google Patents

Pyrimidine derivative and use thereof Download PDF

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US10695347B2
US10695347B2 US15/773,826 US201615773826A US10695347B2 US 10695347 B2 US10695347 B2 US 10695347B2 US 201615773826 A US201615773826 A US 201615773826A US 10695347 B2 US10695347 B2 US 10695347B2
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compound
mmol
added
mixture
reaction
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US20180338973A1 (en
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Xuehai Wang
Yong Xu
Xijun Sheng
Xiaolin Zhang
Hangui Xia
Zhongwen Yang
Yang Yue
Lu HUANG
Qiang Xiao
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HUMANWELL HEALTHCARE (GROUP) Co Ltd
Hubei Bio-Pharmaceutical Industrial Technological Institute Inc
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Hubei Bio-Pharmaceutical Industrial Technological Institute Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5375—1,4-Oxazines, e.g. morpholine
    • A61K31/5377—1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/46—Two or more oxygen, sulphur or nitrogen atoms
    • C07D239/48—Two nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings
    • C07D409/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing three or more hetero rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/08—Solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/12—Aerosols; Foams
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate

Definitions

  • the present disclosure relates to the biological medicine field, in particular to pyrimidine derivative and use thereof, and more particular to pyrimidine derivative, the method of preparing the same and use in preparation of a medicament.
  • the morbidity of lung cancer increased by 56% from 2001 to 2010, during which one out of five patients newly-diagnosed with cancer is a patient affected with lung cancer.
  • lung cancer still ranks at the top in “cancer spectrum” in 2011 issued by Zhejiang Cancer Hospital.
  • the morbidity of lung cancer is seven times more than that for 30 years ago.
  • the individualized therapy targeting NSCLC mainly aims at the epidermal growth factor receptor (EGFR) mutation and the Anaplastic lymphoma kinase (ALK) fusion gene, both of which have definite molecular targets, corresponding target assay technology and commercial target medicine, with clinical efficacy improved obviously.
  • EGFR epidermal growth factor receptor
  • ALK Anaplastic lymphoma kinase
  • Anaplastic lymphoma kinase is a receptor tyrosine kinase, which is originally found in a subtype of anaplastic large cell lymphoma (ALCL) and thus is denominated as the Anaplastic Lymphoma kinase (Science, 1994, 263, 1281-1284; Oncogene, 1994, 9, 1567-1574).
  • ALK fusion gene is a potent carcinogenic gene that is newly discovered as one of NSCLC-driven gene, with echinoderm microtubule related protein 4 (EML4-ALK) as the most common one.
  • ALK-positive non-small cell lung cancer is a subtype of lung cancer newly discovered in 2007, representing 3% to 5% of total NSCLCs.
  • the secondary resistance is at least due to self-secondary mutation of the ALK kinase, including L1196M, G1269A, S1206Y, G1202R, 1151Tins, L11152R and C1156Y, with L1196M and G1269A detected at highest frequency in clinic. It has been revealed by researchers that the secondary mutation of ALK kinase is found in about one third of Crizotinib-resistant patients and no obvious progress is observed in about 40% ALK-positive patients under continuing therapy with Crizotinib. Moreover, it is also observed from two clinical trials for Crizotinib that the most common side effects are visual impairment, nausea, diarrhea, vomiting, edema and constipation, with an occurring rate above 25%.
  • LDK378 still exhibits 80% response rate in 88 patients with the ALK-positive NSCLCs, who had undergone the Crizotinib therapy, in the early clinical trial, and thus was awarded as “breakthrough” by FDA in March 2013 and approved as Ceritinib (trade name: Zykadia) by FDA in April 2014 to treat patients with ALK-positive NSCLCs who had been resistant to Crizotinib based on inhibition of Crizotinib-resistant mutation by LDK378.
  • LDK378 inhibits the Crizotinib-resistant mutation, including L1196M, G1269A, 1171T and S1206Y, with G1202R and F1174C excluded, indicating certain limitation.
  • the clinical dosage of the Ceritinib is large, about 750 mg, P.O qday.
  • a novel ALK inhibitor having improved resistance and druggability can be developed by modifying structures of candidate compound, therefore improving bioactivity and bioavailability, which is of greatly importance for diseases caused by the ALK mutation in clinic.
  • bioactivity and bioavailability which is of greatly importance for diseases caused by the ALK mutation in clinic.
  • Embodiments of the present disclosure seek to solve at least one of the problems existing in the related art at least to some extent, or to provide a useful commercial alternative at least.
  • the present disclosure provides in embodiments a pharmaceutical compound for treating a cancer.
  • R 1 is selected from 5- or 6-membered cycloalkyl, 5- or 6-membered heterocyclyl, 5- or 6-membered aryl, or 5- or 6-membered heteroaryl, optionally said 5- or 6-membered cycloalkyl, 5- or 6-membered heterocyclyl, 5- or 6-membered aryl and 5- or 6-membered heteroaryl each are independently substituted with one or more substituents chosen from halogen, hydroxyl, cyano, nitro, C 1-8 alkyl, C 2-8 alkenyl, C 2-8 alkynyl, C 3-8 cycloalkyl, 3- to 8-membered heterocyclyl, C 5-10 aryl, 5- to 10-membered heteroaryl, C 1-6 alkoxy, C 3-8 cycloalkoxy, —S(O) p R 6 .
  • R 2 is selected from hydrogen, halogen, C 1-6 alkyl, C 1-6 alkoxy, halogen substituted C 1-6 alkyl, or halogen substituted C 1-6 alkoxy;
  • R 3 is selected from optionally substituted piperazinyl, optionally substituted morpholinyl, optionally substituted piperidyl, optionally substituted cyclohexylaminyl, optionally substituted C 1-2 alkyl or optionally substituted 1,2,3,6-tetrahydropyridyl, wherein a substitution on substituted C 1-2 alkyl is optionally substituted piperidyl;
  • R 4 is hydrogen or C 1-6 alkyl
  • R 5 is selected from hydrogen, chlorine, hydroxyl, cyano, C 1-6 alkyl, C 1-6 alkoxy, or halogen substituted C 1-6 alkoxy.
  • R 1 is selected from 5- or 6-membered heterocyclyl, 5- or 6-membered aryl, or 5- or 6-membered heteroaryl substituted with one or more —S(O) p R 6 .
  • R 2 is selected from hydrogen, halogen, C 1-6 alkyl, C 1-6 alkoxy, or halogen substituted C 1-6 alkoxy.
  • R 3 is selected from optionally substituted piperazinyl, optionally substituted morpholinyl, optionally substituted piperidyl, optionally substituted methyl or optionally substituted 1,2,3,6-tetrahydropyridyl, with a substitution on the optionally substituted methyl being optionally substituted piperidyl, wherein said optionally substituted piperazinyl, optionally substituted morpholinyl, optionally substituted piperidyl, or optionally substituted 1,2,3,6-tetrahydropyridyl is optionally substituted with one or more substituents chosen from C 1-6 alkyl, hydroxyl, C 1-6 alkylamino, C 1-6 alkoxy, oxo ( ⁇ O), C 1-6 acyl, morpholinyl, C 1-6 alkylmorpholinyl, piperazinyl, C 1-6 alkylpiperazinyl, C 1-6 acylpiperazinyl, hydroxyl C 1-6 alkyl
  • R 4 is hydrogen or C 1-4 alkyl.
  • R 5 is chlorine or halogen substituted C 1-6 alkoxy.
  • R 6 is C 1-4 alkyl.
  • p is 2.
  • R 1 is any one of
  • R 2 is selected from hydrogen, chlorine, methyl, methoxy, ethoxy, isopropoxy or difluoromethoxy.
  • R 3 is any one selected from
  • R 1 is
  • R 3 can be chosen from any one selected from
  • R 4 is hydrogen or methyl.
  • R 5 is chlorine or difluoromethoxy.
  • the compound of formula I is any one selected from
  • C 1-6 is chosen from C 1 , C 2 , C 3 , C 4 , C 5 and C 6 ;
  • C 1-8 is chosen from C 1 , C 2 , C 3 , C 4 , C 5 , C 6 , C 7 and C 8 ;
  • C 2-8 is chosen from C 2 , C 3 , C 4 , C 5 , C 6 , C 7 and C 5 ;
  • C 3-8 is chosen from C 3 , C 4 , C 5 , C 6 , C 7 and C 8 ;
  • C 5-10 is chosen from C 5 , C 6 , C 7 , C 8 , C 9 and C 10 .
  • the term “pharmaceutically acceptable” is intended to mean the compounds, materials, compositions and/or dosage forms of the present disclosure are suitable for use in contact with human and animal tissues within a range of reliable medical judgment, without excessive toxicity, irritation, allergic reaction or other problems or complications, thus having a reasonable benefit comparable to their risks.
  • pharmaceutically acceptable salts refer to salts of the compound of the present disclosure prepared by contacting the compound containing one or more designated substituents with a relatively non-toxic acid or base.
  • a relatively non-toxic acid or base For example, when the compound contains relatively acidic functional groups, its base addition salt can be obtained by contacting a neutral form of such compound with an adequate amount of base in a pure solution or a suitable inert solvent. Examples of this pharmaceutically acceptable base addition salt include sodium, potassium, calcium, ammonium, organic amino or magnesium salts or other similar salts.
  • its acid addition salt can be obtained by contacting a neutral form of such compound with an adequate amount of acid in a pure solution or a suitable inert solvent.
  • this pharmaceutically acceptable acid addition salt examples include inorganic acid salts, including, such as hydrochloric acid, hydrobromic acid, nitric acid, carbonic acid, bicarbonate, phosphoric acid, monohydrogen phosphate, dihydrogen phosphate, sulfuric acid, hydrogen sulphate, hydroiodic acid, phosphorous acid and the like; organic acid salts, including, such as acetic acid, propionic acid, isobutyric acid, maleic acid, malonic acid, benzoic acid, succinic acid, suberic acid, fumaric acid, lactic acid, mandelic acid, phthalic acid, benzenesulfonic acid, p-toluenesulfonic acid, citric acid, tartaric acid, methanesulfonic acid and the like; and salts of organic acid, such as salts of amino acid (such as, arginine), glucuronic acid and the like (see, Berge et al., “Pharmaceutical Salts”, Journal of
  • the neutral form of the compound can be regenerated by contacting the salt thereof with a base or acid and then isolating the resulting parent compound which differs with other forms of its salt in some physical properties, such as solubility in polar solvents, using conventional manners.
  • “pharmaceutically acceptable salts” are derivatives of the compound in the present disclosure which was formed by contacting the parent compound with an acid or base.
  • the pharmaceutically acceptable salt include, but are not limited to, inorganic acid or organic acid salts of basic groups (such as amine group), alkali metal or organic salts of acidic groups (such as carboxylic acid) and the like.
  • the pharmaceutically acceptable salts typically are non-toxic, such as the quaternary ammonium salt of the parent compound or salts formed with non-toxic inorganic or organic acids.
  • the non-toxic salts usually include, but are not limited to those derived from inorganic and organic acids which are selected from the group consisting of 2-acetoxybenzoic acid, 2-hydroxyethanesulfonic acid, acetic acid, ascorbic acid, benzenesulfonic acid, benzoic acid, bicarbonate, carbonic acid, citric acid, edetic acid, ethanedisulfonic acid, ethanesulfonic acid, fumaric acid, glucoheptose, gluconic acid, glutamic acid, glycolic acid, hydrobromic acid, hydrochloric acid, hydroiodide, hydroxyl, hydroxynaphthalene, isethionic acid, lactic acid, lactose, dodecylsulfonic acid, maleic acid, malic acid, mandelic acid, methanesulfonic acid, nitric acid, oxalic acid, pamoic acid, pantothenic acid, phenylacetic acid,
  • the pharmaceutically acceptable salts of the present disclosure can be synthesized from the parent compounds containing acidic or basic groups using conventional chemical methods, and typically be prepared by contacting the compound in a free acidic or basic form with a stoichiometric amount of suitable base or acid in water or organic solvents or both, for example, non-aqueous medium such as ether, ethyl acetate, ethanol, isopropanol or acetonitrile.
  • the compound of the present disclosure can also be existed as a prodrug thereof, which is easily to be converted into the present compound by chemical reaction under physiological conditions.
  • Any compounds that can be converted in vivo into the present compounds of formula I to provide bioactivity are within the scope of the present disclosure.
  • a carboxyl-containing compound forms physically-hydrolysable ester, serving as the prodrug which can be hydrolyzed in vivo under physiological conditions to give the present compound of formula I.
  • said prodrug is administered orally, because it is mainly hydrolyzed by digestive enzymes.
  • the prodrug in form of ester can also be administered parenterally, if it is active or is hydrolyzed in blood.
  • the prodrug can be converted to the compound of the present disclosure in vivo by chemical or biochemical methods.
  • the compound of the present disclosure can be existed as a non-solvent or solvate (including hydrate) thereof, generally speaking both of them being comparatively included in the present disclosure.
  • the compound of the present disclosure can also be existed in polycrystalline or amorphous forms.
  • the compound of the present disclosure can contain asymmetric carbon atoms (that is optical center) or double bonds, and all of the racemate, the diastereomer, the geometrical isomer and the individual isomer thereof are included within the scope of the present disclosure.
  • the compound of the present disclosure can contain one or more designated geometrical isomers or stereoisomers, and all of them are contemplated by the applicant, including cis-isomers, trans-isomers, ( ⁇ )-enantiomers, (+)-enantiomers, (R)-enantiomers. (S)-enantiomers, diastereoisomers, (D)-isomers and (L)-isomers, and racemic mixture thereof or other mixture, such as enantiomer or diastereomer enriched mixture, all of the mixtures being within the scope of the present disclosure.
  • Other asymmetric carbon atoms can also be existed in the substituents such as alkyl and the like, and all of the isomers and mixtures thereof are also included within the scope of the present disclosure.
  • Optically active (R)- and/or (S)-isomers may be prepared by using chiral synthons or chiral reagents, or using other conventional techniques, so that a desired enantiomer of the compound in the present disclosure can be prepared by asymmetric synthesis or derivatization with chiral auxiliaries, and specifically including the steps of isolating the resulting enantiomer from the diastereomeric mixture, and then cleaving the auxiliary groups in the enantiomer to provide the desired pure enantiomer.
  • an enantiomer of the compound can be prepared by contacting the compound with an appropriate amount of optically active acids or bases to form a diastereomeric salt, and resolving the diastereoisomer mixture by methods well known in the art such as fractional crystallization or chromatography to separate the enantiomer with the diastereoisomer followed by collection of the separated pure enantiomer.
  • the separation of an enantiomer with a diastereoisomer is usually accomplished by chromatography on chiral stationary phase, and optionally in combination with chemical derivatization method (e.g., carbamate was generated by amine).
  • one or more atoms constituting the present compound may process non-natural proportions of atomic isotopes, and for example, the compound can be labeled with radioisotopes, such as tritium ( 3 H), iodine-125 ( 125 I) or C-14 ( 14 C). All the combinations of isotopes in the compound are included within the scope of the present disclosure, no matter the isotopes are radioactive or not.
  • radioisotopes such as tritium ( 3 H), iodine-125 ( 125 I) or C-14 ( 14 C). All the combinations of isotopes in the compound are included within the scope of the present disclosure, no matter the isotopes are radioactive or not.
  • excipient generally refers to the carrier, diluent and/or medium required to formulate an effective pharmaceutical composition.
  • the term “effective amount” or “therapeutically effective amount” refers to a sufficient amount of a drug or agent that is non-toxic but be capable of achieving the desired effect.
  • the “effective amount” of an active substance in the formulation refers to its amount required to achieve the desired effect when used in association with one or more other active substances.
  • the effective amount varies from person to person, depending on the age and general condition of the receptor and on the designated active substance. The appropriate effective amount in individual cases can be determined by a person skilled in the art in accordance with routine testing.
  • active ingredient refers to a chemical entity which is effective in treating targeted disorders, diseases or conditions.
  • substituted means that at least one hydrogen on the designated atom is replaced with one or more substituents including variants of deuterium and hydrogen, provided that the designated atom's normal valence is not exceeded and the substituted stucture is stable.
  • substituent is ketone group (i.e., ⁇ O)
  • two hydrogens on the atom are replaced, but hydrogens on aromatic group cannot be replaced by ketone group.
  • optionally substituted means that the hydrogens on the designated atom may be substituted or not substituted with one or more substituents unless otherwise specified. The type and number of the substituents are not specifically restricted at the substitution occurrence.
  • any variable e.g., R
  • its definition is independent.
  • the group may be optionally substituted with up to two of R, and R can be different groups at each occurrence.
  • substituents and/or variables are permissible provided that the compound produced is stable.
  • one of the variables is a single bond, it indicated that the two groups to which it is attached are directly connected.
  • L represents a single bond in A-L-Z, it means that A-L-Z is actually A-Z.
  • alkyl In the context of the present disclosure, the terms “alkyl”, “alkane” or “alkyl group” are exchangeable, with the alkyl optionally substituted with one or more substituents described herein. In some embodiments of the present disclosure, the alkyl group contains 1 to 8 carbon atoms. In other embodiments, the alkyl group contains 1 to 6 carbon atoms. In still other embodiments, the alkyl group contains 1 to 4 carbon atoms.
  • alkyl group contains but not limited to methyl (Me, —CH 3 ), ethyl (Et, —CH 2 CH 3 ), n-propyl (n-Pr, —CH 2 CH 3 CH 3 ), isopropyl (i-Pr, —CH(CH 3 ) 2 ), n-butyl (n-Bu, —CH 2 CH 2 CH 2 CH 3 ), isobutyl (i-Bu, —CH 2 CH(CH 3 ) 2 ), sec-butyl (s-Bu, —CH(CH 3 )CH 2 CH 3 ), tert-butyl (t-Bu, —C(CH 3 ) 3 ), n-pentyl (—CH 2 CH 2 CH 2 CH 2 CH 3 ), 2-pentyl (—CH(CH 3 )CH 2 CH 2 CH 3 ), 3-pentyl (—CH(CH 2 CH 3 ) 2 ), 2-methyl-2-butyl (—C(CH 3 ) 2 CH CH 3
  • alkoxy refers to an alkyl group, as previously defined, attached to the rest moiety of molecule via an oxygen atom. Unless otherwise specified, the alkoxy group contains 1 to 6 carbon atoms. Examples of the alkoxy group include but not limited to methoxy (MeO, —OCH 3 ), ethyoxy (EtO, —OCH 2 CH 3 ), propoxy (n-PrO, n-propoxy, —OCH 2 CH 2 CH 3 ), isopropoxy (i-PrO, i-propoxy, —OCH(CH 3 ) 2 ), n-butoxy (n-BuO, n-butoxy, —OCH 2 CH 2 CH 2 CH 3 ), 1-methylpropoxy (s-BuO, s-butoxy, —OCH(CH 3 )CH 2 CH 3 ), 2-methyl-1-propoxy (i-BuO, i-butoxy, —OCH 2 CH(CH 3 ) 2 ), tert-butoxy (
  • cycloalkyl refers to monovalent or multivalent saturated monocyclic, bicyclic, or tricyclic systems containing 3 to 8 carbon atoms.
  • the cycloalkyl group can be optionally substituted with one or more substituents described herein.
  • the cycloalkyl contains 3 to 8 carbon atoms.
  • Examples of the cycloalkyl group include but not limited to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl and so on.
  • halogen refers to fluorine (F), chlorine (Cl), bromine (Br) or iodine (I).
  • alkylamino includes “N-alkylamino” and “N,N-dialkylamino”, in which the amino group each is independently substituted with one or two alkyl groups, as previously defined.
  • An appropriate alkylamino group can be monoalkylamino or dialkylamino.
  • alkylamino examples include but not limited to N-methylamino, N-ethylamino, isopropylamino, propylamino, tert-butylamino, n-butylamino, I-methylpropylamino, n-pentylamino, n-hexylamino, N,N-dimethylamino, N,N-diethylamino and so on.
  • C 1-6 acyl refers to R—C( ⁇ O)—, in which R is C 1-6 alkyl, as previously defined.
  • alkylmorpholinyl refers to morpholinyl substituted with one or more alkyl groups.
  • C 1-6 acylpiperazinyl refers to piperazinyl substituted with one or more C 1-6 acyl groups.
  • hydroxyl C 1-6 alkylpiperazinyl refers to C 1-6 alkyl substituted piperazinyl which is substituted with one or more hydroxyl groups.
  • alkylaminopiperidyl refers to amino substituted piperidyl which is substituted with one or more alkyl groups.
  • reaction solvent used in each reaction step of the present disclosure is not particularly limited, and any solvent that can dissolve a starting material to some extent and does not inhibit the reaction is included.
  • similar modifications, alternatives, or equivalents of the solvents, solvent combinations, and proportions thereof described herein also belong to the scope of the present disclosure.
  • the present disclosure provides in embodiments a method of preparing a compound represented in formula I.
  • a general method of synthesizing such compounds includes synthesizing a parent pyrimidine compound and then connecting to a nitrogen-containing compound via a carbon chain.
  • the present disclosure provides a method of preparing a compound represented in formula I.
  • Said method includes the following steps: substitution reaction between the compound of formula 5A and the compound of formula 4A; and then conversion to a corresponding compound of formula I in accordance with different substitutions or a protective group used, thus giving the compound of formula I,
  • R 1 is selected from 5- or 6-membered cycloalkyl, 5- or 6-membered heterocyclyl, 5- or 6-membered aryl, or 5- or 6-membered heteroaryl, and optionally said 5- or 6-membered cycloalkyl, 5- or 6-membered heterocyclyl, 5- or 6-membered aryl and 5- or 6-membered heteroaryl each are independently substituted with one or more substituents chosen from halogen, hydroxyl, cyano, nitro, C 1-8 alkyl, C 2-8 alkenyl, C 2-8 alkynyl, C 3-8 cycloalkyl, 3- to 8-membered heterocyclyl, C 5-10 aryl, 5- to 10-membered heteroaryl, C 1-6 alkoxy, C 3-8 cycloalkoxy, —S(O) p R 6 , —C(O)R 6 , —C(O)OR 6 , —NR 7 R 8 or —C(O)NR
  • R 2 is selected from hydrogen, halogen, C 1-6 alkyl, C 1-6 alkoxy, halogen substituted C 1-6 alkyl, or halogen substituted C 1-6 alkoxy;
  • R 3 is selected from optionally substituted piperazinyl, optionally substituted morpholinyl, optionally substituted piperidyl, optionally substituted cyclohexylaminyl, optionally substituted C 1-2 alkyl, or optionally substituted 1,2,3,6-tetrahydropyridyl, with a substitution on substituted C 1-2 alkyl being optionally substituted piperidyl;
  • R 4 is hydrogen or C 1-6 alkyl
  • R 5 is selected from hydrogen, chlorine, hydroxyl, cyano, C 1-6 alkyl, C 1-6 alkoxy, or halogen substituted C 1-6 alkoxy.
  • R 1 is selected from 5- or 6-membered heterocyclyl, 5- or 6-membered aryl or 5- or 6-membered heteroaryl substituted with one or more —S(O) p R 6 .
  • R 2 is selected from hydrogen, halogen, C 1-6 alkyl, C 1-6 alkoxy, or halogen substituted C 1-6 alkoxy.
  • R 3 is selected from optionally substituted piperazinyl, optionally substituted morpholinyl, optionally substituted piperidyl, optionally substituted methyl or optionally substituted 1,2,3,6-tetrahydropyridyl, with a substitution on the optionally substituted methyl being optionally substituted piperidyl, in which said optionally substituted piperazinyl, optionally substituted morpholinyl, optionally substituted piperidyl, or optionally substituted 1,2,3,6-tetrahydropyridyl is optionally substituted with one or more substituents chosen from C 1-6 alkyl, hydroxyl, C 1-6 alkylamino, C 1-6 alkoxy, oxo ( ⁇ O), C 1-6 acyl, morpholinyl, C 1-6 alkylmorpholinyl, piperazinyl, C 1-6 alkylpiperazinyl, C 1-6 acylpiperazinyl, hydroxyl C 1-6 alkyl C 1-6 alkyl,
  • R 4 is hydrogen or C 1-6 alkyl.
  • R 5 is chlorine or halogen substituted C 1-6 alkoxy.
  • R 6 is C 1-4 alkyl.
  • p is 2.
  • R 1 is any one of
  • R 2 is selected from hydrogen, chlorine, methyl, methoxy, ethoxy, isopropoxy or difluoromethoxy.
  • R 3 is any one selected from
  • R 3 is any one of
  • R 4 is hydrogen or methyl.
  • R 5 is chlorine or difluoromethoxy.
  • the present compound of formula I is synthesized by a scheme below:
  • R 1 , R 2 , R 3 , R 4 and R 5 are defined as described in the present disclosure.
  • the compound of formula I can be synthesized by a displacement reaction between the compound of formula II and the compound of formula III, which is carried out by contacting such two compounds in an organic solvent in the presence of p-toluenesulfonic acid.
  • the organic solvent can be n-butanol, which provides a suitable reaction condition for the compound of formula II and the compound of formula III, thereby contributing to improving yield of a target product and reaction efficiency, with side reaction reduced.
  • the compound of formula I is synthesized by a scheme below:
  • the compound of formula I-23 is synthesized by a scheme below:
  • the compound of formula I-90 is synthesized by a scheme below:
  • the compound of formula I-89 is synthesized by a scheme below
  • the compound of formula I can be synthesized rapidly and effectively by the method of the present disclosure, resulting in a target product with high yield and purity.
  • the method is useful for industrialization owing to accessible raw materials, simple operation and post-treatment, and is environment-friendly as well.
  • the present disclosure provides in embodiments a pharmaceutical composition, including a therapeutically effective amount of the compound descried in the first aspect.
  • composition used herein includes one or more compounds of the present disclosure, a physiological/pharmaceutically acceptable salt or a prodrug thereof in combination with other chemical components such as a physiological/pharmaceutically acceptable carrier or excipient.
  • the pharmaceutical composition is useful in administering to a subject, increasing absorption of active ingredients and improving their biological activities.
  • the pharmaceutical composition of the present disclosure includes the compound of formula I described above.
  • the pharmaceutical composition is for inhibiting kinase, such as anaplastic lymphoma kinase, treating or preventing a cancer and/or suppressing proliferation of cancer cells.
  • the cancer is lung cancer or anaplastic large cell non-Hodgkin lymphoma, for example the lung cancer is non-small cell lung cancer.
  • the pharmaceutical composition further includes a second therapeutic agent, in which said second therapeutic agent is different from the compound described above and said second therapeutic agent is useful in inhibiting kinase, such as anaplastic lymphoma kinase, treating or preventing a cancer or suppressing proliferation of cancer cells.
  • the second therapeutic agent is administered in combination with the compound described above, such that the pharmaceutical composition is more useful in inhibiting kinase, such as anaplastic lymphoma kinase, treating or preventing a cancer or suppressing proliferation of cancer cells.
  • the pharmaceutical composition further includes a pharmaceutically acceptable carrier, excipient, diluent, auxiliary, vehicle, or combination thereof.
  • the pharmaceutical composition is in the form of tablets, capsules, injections, powder-injections, powders, syrups, solutions, suspensions or aerosols, thereby improving applicability of the pharmaceutical composition significantly.
  • the pharmaceutical composition described in the above embodiments can be existed in a suitable solid or liquid carrier or a diluent, or a suitable disinfector used for injection or instillation.
  • the various dosage forms of the present pharmaceutical composition can be prepared via general methods in the medical art.
  • the present compound or the present pharmaceutical composition can be applied clinically to mammal, including human and animals via various administration routes, such as mouth, nose, skin, lung or gastrointestinal tract and so on. No matter how the compound or the pharmaceutical composition is administered, the best individual dosage depends on particular treatments. In general, the dosage starts from low dose and increases gradually until a most suitable dosage is achieved. For example, the compound or the pharmaceutical composition is administered orally.
  • the present disclosure provides in embodiments use of the compound in the first aspect, the compound prepared by the method in the second aspect or the pharmaceutical composition in the third aspect in preparation a medicament.
  • the medicament is for inhibiting kinase, such as anaplastic lymphoma kinase, treating or preventing a cancer or suppressing proliferation of cancer cells.
  • kinase such as anaplastic lymphoma kinase
  • the present compound of formula I inhibits anaplastic lymphoma kinase (ALK), particularly ALKL1196M kinase significantly, and suppresses proliferation of Karpas299 cells potently.
  • ALK anaplastic lymphoma kinase
  • the present compound of formula I also suppresses proliferation of Ba/F3 EML4-ALK cells potently.
  • the present compounds compared to positive control compounds Crizotinib and Ceritinib (LDK378), the present compounds outperform in water solubility according to Kinetic solubility tests.
  • the present compound exhibits outstanding metabolic stability according to metabolic stability tests in vitro, thereby supporting its preclinical study greatly.
  • the present compound has better membrane permeability over control compounds in a Caco-2 experiment, thus the present compound is easier to be absorbed in intestinal tracts with higher bioavailability than the control compounds.
  • the present compound can be used as an ALK inhibitor in preparation of an antitumor medicament for inhibiting anaplastic lymphoma kinase.
  • the present compound of the formula I is for use in preparation of a medicament for treating a cancer, in which the cancer is lung cancer or anaplastic large cell non-Hodgkin lymphoma, for example, the cancer is non-small cell lung cancer.
  • the medicament prepared according to embodiments of the present disclosure can be used as an ALK inhibitor in treating one or more tumors related to activity of anaplastic lymphoma kinase (ALK) efficiently, said tumor including but not limited to lung cancer.
  • ALK anaplastic lymphoma kinase
  • the pyrimidine derivative disclosed herein represented in formula I, as an ALK inhibitor, can have a promising clinical and medical application.
  • the present disclosure provides in embodiments that the compound or the pharmaceutical composition described above is for use in inhibiting kinase, such as anaplastic lymphoma kinase, treating or preventing a cancer or suppressing proliferation of cancer cells.
  • kinase such as anaplastic lymphoma kinase
  • the present disclosure provides in embodiments a method of inhibiting kinase, such as anaplastic lymphoma kinase, treating or preventing a cancer or suppressing proliferation of cancer cells.
  • said method includes administering to a subject a therapeutically effective amount of the compound or the pharmaceutical composition described above.
  • the present disclosure provides in embodiments a method of treating a tumor related to activity of anaplastic lymphoma kinase (ALK).
  • said method includes administering to a subject a therapeutically effective amount of the compound or the pharmaceutical composition described above.
  • the present disclosure provides in examples a compound of formula I or a pharmaceutically acceptable salt, a hydrate, a solvate, a metabolite or a prodrug thereof, a method of preparing the same, an intermediate or a pharmaceutical composition including the same, or use of the compound or the pharmaceutical composition in preparation of a medicament.
  • Zinc powder (1.6 g) was added in portions, and the mixture was stirred at room temperature for 1 hour. After TLC indicated the reaction was completed, the reaction mixture was filtered and concentrated under reduced pressure to give a crude product, which was further isolated by column chromatography to obtain a compound 4A-1 (600 mg, yield 82.3%).
  • Zinc powder (1.6 g) was added in portions, and the mixture was stirred at room temperature for 1 hour. After TLC indicated the reaction was completed, the reaction mixture was filtered and concentrated under reduced pressure to give a crude product, which was further isolated by column chromatography to obtain a compound 4A-2 (420 mg, yield 88.97%).
  • Zinc powder (1.6 g) was added in portions, and the mixture was stirred at room temperature for 1 hour. After TLC indicated the reaction was completed, the reaction mixture was filtered and concentrated under reduced pressure to give a crude product, which was further isolated by column chromatography to obtain a compound 4A-3 (406 mg, yield 80.8%).
  • Zinc powder (1.6 g) was added in portions, and the mixture was stirred at room temperature for 1 hour. After TLC indicated the reaction was completed, the reaction mixture was filtered and concentrated under reduced pressure to give a crude product, which was further isolated by column chromatography to obtain a compound 4A-4 (536 mg, yield 80.98%).
  • Zinc powder (1.6 g) was added in portions, and the mixture was stirred at room temperature for 1 hour. After TLC indicated the reaction was completed, the reaction mixture was filtered and concentrated under reduced pressure to give a crude product, which was further isolated by column chromatography to obtain a compound 4A-5 (450 mg, yield 78.7%).
  • Zinc powder (1.6 g) was added in portions, and the mixture was stirred at room temperature for 1 hour. After TLC indicated the reaction was completed, the reaction mixture was filtered and concentrated under reduced pressure to give a crude product, which was further isolated by column chromatography to obtain a compound 4A-6 (411 mg, yield 80.4%).
  • Zinc powder (1.6 g) was added in portions, and the mixture was stirred at room temperature for 1 hour. After TLC indicated the reaction was completed, the reaction mixture was filtered and concentrated under reduced pressure to give a crude product, which was further isolated by column chromatography to obtain a compound 4A-7 (566 mg, yield 81.8%).
  • Zinc powder (1.6 g) was added in portions, and the mixture was stirred at room temperature for 1 hour. After TLC indicated the reaction was completed, the reaction mixture was filtered and concentrated under reduced pressure to give a crude product, which was further isolated by column chromatography to obtain a compound 4A-8 (650 mg, yield 85.4%).
  • Compound I-110 was synthesized with the method similar to that of Example 36.
  • Step 2.2 Further dilute said compound dilution by 3-fold in series, thus obtain 10 concentrations.
  • Step 2.3 Add 100 ⁇ l of 100% DMSO to two empty wells for no compound control and no enzyme control in the same 96-well plate. Mark the plate as source plate.
  • A1 of the 96-well plate is transferred to A1 and A2 of the 384-well plate.
  • A2 of the 96-well plate is transferred to A3 and A4 of the 384-well plate, and so on.
  • A means the IC 50 value of compound is below 1 nM
  • B means the IC 50 value of the compound is 1 nM to 10 nM
  • C means the IC 50 value of the compound is above 10 nM.
  • a means the IC 50 value of the compound is below 1 nM
  • b means the IC 50 value of the compound is 1 nM to 10 nM
  • c means the IC 50 value of the compound is above 10 nM.
  • the living cells in culture are able to be quantified by determining the ATP level.
  • a living-cell testing kit to quantify the living cells, which utilizes UltraGlow luciferase to generate a stable glow signal in the presence of ATP which is produced by metabolism of living cells, such as respiration.
  • a reagent CellTiter-GloTM was added into cell culture media, and then the luminescent intensity was determined to measure proliferation of cells, as the luminescent intensity is in direct proportion to the ATP level which correlates positively to the number of living cells.
  • the test plate was tested with the Envision (PE).
  • Cell media RPMI-1640 medium, fetal calf serum (FCS), antibiotics (Penicillin-Streptomycin)
  • Assay reagent Cell Viability Assay Kit CellTiter-Glo
  • Karpas 299 cells were plated into a black 384-well plate, with 2500 cells in 45 ⁇ l cell suspension per well. The plate was placed in a carbon dioxide incubator overnight.
  • Each compound to be tested was dissolved in DMSO at 10 mM as the stock solution, which was left in a nitrogen-gas tank for storage.
  • Step 3.3 Mixing the compound with the test cells
  • Table 2 shows that the present compounds suppress proliferation of Karpas299 cells. It should be noted that in the results of Table 2, “D” means the IC 50 value of the compound is below 50 nM; the “E” means IC 50 value of the compound is 50 nM to 100 nM; and “F” means the IC 50 value of the compound is above 50 nM.
  • Cell media RPMI-1640 medium, fetal calf serum (FCS), antibiotics (Penicillin-Streptomycin), IL-3, puromycin
  • the living cells in medium are able to be quantified by determining the ATP level.
  • a cell viability assay kit to quantify the living cells, which utilizes UltraGlow luciferase to generate a stable glow signal in the presence of ATP which is produced by metabolism of living cells, such as respiration.
  • a reagent CellTiter-GloTM was added into cell culture media, and then the luminescent intensity was determined to measure proliferation of cells, as the luminescent intensity is in direct proportion to the ATP level which correlates positively to the number of living cells.
  • the test plate was assayed by the Envision (PE).
  • Ba/F3 EML4-ALK cells were planted into a 384-well plate, with almost 300 cells per well. The plate was placed in a carbon dioxide incubator overnight.
  • Step 3.3 Mixing the compound to be tested with the test cells
  • the compounds to be tested at individual working concentrations were transferred into corresponding wells in the 384-well plate in the step 3.1, with 5 ⁇ M as the initial working concentration, and then the 384-well plate was incubated in the carbon dioxide incubator for 3 days.
  • Table 3 shows that the present compounds suppress proliferation of the Ba/F3 EML4-ALK cells.
  • a quantified compound was dissolved with 100% DMSO at a final concentration of 10 mM, 10 ⁇ L of the test compound and a control compound (10 mM in DMSO) each were added into individual wells (490 ⁇ L buffer/well) of a 96-well plate. Afterwards, the 96-well plate was oscillated for 2 minutes, and incubated in the oscillator at a temperature of 22 ⁇ 2° C. for 24 hours. 200 ⁇ L of the test solution was transferred to a MultiScreen filter plate which is a polycarbonate membrane, and filtered with a millipore vacuum manifold to give the filtrate, which was further measured with the HPLC-UV detector. Three concentrations of UV-standards and the test compound were detected simultaneously in duplicate, and then the average concentration of the test compound was calculated via a standard curve.
  • Clearance rate of compound in phase I reaction was evaluated in metabolic stability assay in vitro, and based on the clearance rate its intrinsic clearance rate in hepatocyte in vivo was predicted.
  • the metabolic stabilities of the present compounds in liver microsomes of human and rat were evaluated in metabolic stability assay in vitro.
  • the controls are crizotinib and Ceritinib LDK378.
  • Buffer medium 100 nM Potassium phosphate buffer, PH 7.4; 10 mM MgCl 2
  • Stop buffer A solution of 100 ng/mL Tolbutamide and 100 ng/mL Labetalol (as internal standards) in cooled cyanomethane (CAN)
  • step 1.1 50 ⁇ L of the intermediate solution obtained in step 1.1 was diluted with 450 ⁇ L of Potassium phosphate buffer (100 mM) (Conc.: 10 ⁇ M, 4.5% methanol).
  • liver microsomal solution (0.625 mg/mL) was added into individual wells obtained in the step 2.1 with the liquid platform, with a final concentration of 0.5 mg/mL.
  • each well was added with 10 ⁇ L potassium phosphate buffer (100 mM), and then stilled for 60 minutes, after which 300 ⁇ L CAN stop buffer containing 100 ng/mL Tolbutamide was added, thus obtaining a control sample.
  • each well was added with 10 ⁇ L of the NADPH cofactor solution containing 1 mM NADP, 1 mM magnesium chloride, 6.5 mM Isocitric acid and 1 unit/mL isocitrate dehydrogenase to initiate the metabolic reaction.
  • the NADPH cofactor solution containing 1 mM NADP, 1 mM magnesium chloride, 6.5 mM Isocitric acid and 1 unit/mL isocitrate dehydrogenase to initiate the metabolic reaction.
  • 300 ⁇ L CAN stop buffer was added, thus obtaining experimental results not undergone the metabolic reaction.
  • test samples obtained in the step 2.8 were analyzed with the LC-MS/MS detector.
  • Caco-2 cells which are human colonic cancer cells, are wildly used as a model in vitro to study the absorption rate on small intestine.
  • the monolayer of Caco-2 cell has been generally applied to evaluate the passive diffusion and active transport during the intestinal absorption.
  • the membrane permeability of the compounds was evaluated via Caco-2 cell monolayer model in the presence of GF120918A, a strong inhibitor of efflux transporter including Pglycoprotein (P-gp) and breast cancer resistance protein (BCRP), and the absorption of the compounds on the small intestine was predicted.
  • the controls are crizotinib and Ceritinib (LDK378).

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AU2016349089B2 (en) 2019-06-20
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